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THE™ His Tag Antibody [HRP], MAb, Mouse

Western blot analysis of His-tagged protein using THETM His Tag Antibody [HRP], mAb, Mouse (A00612, 1 µg/ml) The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)

THE™ His Tag Antibody [HRP], mAb, Mouse

THE™ His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
A00612
$410.00

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Overview
Specificity THE™ His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH
Conjugate Horseradish peroxidase (HRP)

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.5 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 6G2A9

Examples
  • THE™ His Tag Antibody [HRP], MAb, Mouse
    • THE™ His Tag Antibody [HRP], MAb, Mouse

    Western blot analysis of His-tagged protein using THETM His Tag Antibody [HRP], mAb, Mouse (A00612, 1 µg/ml) The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)


Background
Target Background GenScript THE™ His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column.This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Synonyms His-tag Antibody [HRP],mAb, Mouse;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.