Catalog Products » Antibodies » THE Elite Antibodies » THE™ DYKDDDDK Tag Antibody, mAb, Mouse
THE™ DYKDDDDK Tag Antibody, MAb, Mouse

Comparison of THETM DYKDDDDK Tag Antibody, mAb, Mouse (A: GenScript, A00187, 1 µg/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 µg/ml) by Western blot.
Lane 1 N-terminal DYKDDDDK-tagged fusion protein
Lane 2 Internal DYKDDDDK-tagged fusion protein
Lane 3 C-terminal DYKDDDDK-tagged fusion protein
Predicted Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
Observed Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

Loading:
Lane 1-3 Multiple Tag (Purified) (GenScript, Cat.No.M0101, 400 ng, 80 ng,16 ng)
Lane 4. 293 cell lysate 10 μl
Primary antibody:
A. 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (GenScript, Cat. No. A00187, Lot No. A213902)
B. 1 μg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2)
Secondary antibody:
IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

Western blot analysis of E.coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates.
1: E.coli lysate containing DYKDDDDK-tagged fusion protein
2: Immunoprecipitates of free E.coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.
3: Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins._x000D_
A00187
$139.00

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Overview
Specificity THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Immunoprecipitation (IP) 1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF) 1 µg/ml
Flow Cytometry 1 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b,κ
Clonality Monoclonal
Clone ID 5A8E5
Note GenScript can offer this product per customer's reuqest including product size, buffer components,etc.

Examples
  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Comparison of THETM DYKDDDDK Tag Antibody, mAb, Mouse (A: GenScript, A00187, 1 µg/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 µg/ml) by Western blot.
    Lane 1 N-terminal DYKDDDDK-tagged fusion protein
    Lane 2 Internal DYKDDDDK-tagged fusion protein
    Lane 3 C-terminal DYKDDDDK-tagged fusion protein
    Predicted Size:
    N-terminal DYKDDDDK-tagged fusion protein 52 kD
    Internal DYKDDDDK-tagged fusion protein 55 kD
    C-terminal DYKDDDDK-tagged fusion protein 55 kD
    Observed Size:
    N-terminal DYKDDDDK-tagged fusion protein 52 kD
    Internal DYKDDDDK-tagged fusion protein 55 kD
    C-terminal DYKDDDDK-tagged fusion protein 55 kD
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Loading:
    Lane 1-3 Multiple Tag (Purified) (GenScript, Cat.No.M0101, 400 ng, 80 ng,16 ng)
    Lane 4. 293 cell lysate 10 μl
    Primary antibody:
    A. 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (GenScript, Cat. No. A00187, Lot No. A213902)
    B. 1 μg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2)
    Secondary antibody:
    IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Western blot analysis of E.coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates.
    1: E.coli lysate containing DYKDDDDK-tagged fusion protein
    2: Immunoprecipitates of free E.coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.
    3: Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    293 cells transfected with C-terminal DYKDDDDK tag protein
    Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
    Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    293 cells transfected with N-terminal DYKDDDDK tag protein
    Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
    Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
    The assay was performed with DYKDDDDK-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.


Background
Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Synonyms Mouse anti DYKDDDDK-tag mAb;Mouse anti flag-tag mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.