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THE™ His Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
1. Positive control containg His fusion protein
2. Negative control – IP with isotype control antibody (A01007)
3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)

THE™ His Tag Antibody, mAb, Mouse

THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
A00186
$180.00

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Description

long description：THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Synonyms

Synonyms: THE™ His Tag Antibody, mAb, Mouse

Note

Selling Note: THE™ His Tag Antibody, mAb, Mouse

Overview
Specificity THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-0.2 µg/ml
Immunoprecipitation (IP) 1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF) 1 µg/ml
Flow Cytometry 1 µg/ml
Time-resolved fluorescence energy transfer (TR-FRET) User-optimized
Surrogate Virus Neutralization Test (sVNT) 10 U/ml - 1,200 U/ml
Other applications User-optimized

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 6G2A9
Note GenScript can offer this product according to your requirement, including product size, buffer components,etc.

Examples
  • THE™ His Tag Antibody, MAb, Mouse
    • THE™ His Tag Antibody, MAb, Mouse

    Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
    1. Positive control containg His fusion protein
    2. Negative control – IP with isotype control antibody (A01007)
    3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)


Background
Target Background Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen.After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity.Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.THE™ His Tag mAb is produced from mice ascites and purified by protein A affinity column.This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins.The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells.THE™ His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.THE™ His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Synonyms THE™ Anti-His mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.