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Trx-tag Antibody, mAb, Mouse

This antibody reacts with the Thioredoxin fusion protein from E.coli and human.Not yet tested in other species.
A00180
$99.00

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Overview
Specificity This antibody reacts with the Thioredoxin fusion protein from E.coli and human.Not yet tested in other species.
Host Species Mouse
Immunogen Purified recombinant Trx-tag fusion protein
Species Reactivity E.coli and human
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.1-1.0 µg/ml
Western Blot 1.0 µg/ml
Western Blot 1:100-1:1,000

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a
Clonality Monoclonal
Clone ID 4C12H10

Background
Target Background Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen.Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Synonyms Mouse Anti-Trx-tag;Anti-Trx-tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.