His tagged protein detection & purification
His-tag is one of the most widely applied tags for recombinant protein expression and purification. It is also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. The three new high quality mAb based assay products will provide convenient, easy, and robust tools for different purposes, including purification and detection of His-tagged fusion-protein for downstream assays, enrichment and screening of His-tagged fusion-protein mutants, and Co-IP & screening of interacting proteins.
His Tag ELISA Detection Kit
GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. This kit also offers a His-tagged protein stock for customer recovery verification assay.
Applications
- Detect His-tagged proteins.
- Screen optimal expression conditions.
- Semi-quantitation with known His-tagged protein as standards.
Example
| Conc. of His Tag Standard | OD450 | |||
|---|---|---|---|---|
| (ng/ml) | (pmol/ml) | Duplicate 1 | Duplicate 2 | Average |
| 0 | 0 | 2.177 | 2.183 | 2.18 |
| 1 | 0.088 | 1.833 | 1.831 | 1.832 |
| 3 | 0.265 | 1.374 | 1.418 | 1.396 |
| 9 | 0.796 | 0.825 | 0.922 | 0.874 |
| 27 | 2.389 | 0.516 | 0.444 | 0.48 |
| 81 | 7.168 | 0.266 | 0.289 | 0.278 |
| 243 | 21.504 | 0.158 | 0.157 | 0.158 |
| 729 | 64.513 | 0.105 | 0.101 | 0.103 |
Typical assay results and standard curve using His Tag ELISA Detection Kit
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00436 |
His Tag ELISA Detection Kit - 1 kit |
$298.00 |
His Tag Antibody Plate
His Tag Antibody Plate is a microtiter plate coated with THE™ Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00440b |
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates |
$179.00 |
|
L00440c |
His Tag Antibody Plate (clear, 8 wells × 12 strips) - 5 plates |
$179.00 |
|
L00440w |
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates |
$179.00 |
Other Tagged Protein Detection & Purification
DYKDDDDK(Flag) Tag Antibody Plate (96-well)
DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THE™ Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00455B |
DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates |
$229.00 |
|
L00455C |
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates |
$229.00 |
|
L00455W |
DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates |
$229.00 |
Related products
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00223-500 |
High Affinity Ni-Charged Resin - 500 ml Resin (Total 1000 ml) |
$2480.00 |
|
L00223-50 |
High Affinity Ni-Charged Resin - 50 ml |
$1200.00 |
|
L00223-25 |
High Affinity Ni-Charged Resin - 25 ml Resin (Total 50 ml) |
$168.00 |
|
L00223-10 |
High Affinity Ni-Charged Resin - 10 ml Resin (Total 20 ml) |
$80.00 |
|
L00295-80 |
Ni-charged MagBeads - 80 ml (20 ml Settled Beads) |
$787.50 |
|
L00295 |
Ni-charged MagBeads - 8 ml (2 ml Settled Beads) |
$98.00 |
|
A00174-40 |
His-tag Antibody, pAb, Rabbit - 40 μg |
$99.00 |
|
A00186-40 |
THE™ His Tag Antibody, mAb, Mouse - 40 μg |
$180.00 |
|
A00186-200 |
THE™ His Tag Antibody, mAb, Mouse - 200 μg(5x40μg) |
$675.00 |
|
A00205-40 |
His-tag antibody [Biotin], pAb, Rabbit - 40 μg |
$99.00 |
|
A00612 |
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 μg |
$410.00 |
|
A00613 |
THE™ His Tag Antibody [Biotin], mAb, Mouse - 100 μg |
$410.00 |
|
A01620 |
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 μg |
$410.00 |
