cas9-enzymes_2022
GenCRISPR™ Nucleases
Complexing a CRISPR nuclease with the guide RNA, commonly referred to as the ribonucleoprotein (RNP) complex, has been shown to be the leading CRISPR gene editing mechanism. RNP complex provides scientists with enhanced gene editing performance, while reducing common challenges observed with other delivery systems, including viral- and plasmid-based. GenCRISPR™ nuclease offerings provide scientists with RNA and DNA editing solutions with proven performance including Cas9, eSpCas9, Cas9-EGFP, D10A Nickase, Cas12a and Cas13a.
| GenCRISPR™ Nucleases | |||
|---|---|---|---|
| Cas9 | Cas12a | Cas13a | |
| Targeted Nucleic Acid | ds DNA | ds or ss DNA | ss RNA |
| Size of the protein | ~1,000 – 1,600 AA | ~1,300 AA | ~1,400 AA |
| Guide | tracrRNA + crRNA | crRNA | crRNA |
| Type of cut | Double-strand, blunt cut | Double-strand, staggered cut | ss RNA |
| Cleavage | Cis-cleavage | Cis-cleavage Trans-cleavage |
Cis-cleavage Trans-cleavage |
| Targeting restriction | GC-rich sequences | AT-rich sequences | - |
| PAM/PFS | 5' - NGG - 3' | 5′-TTTV-3′ upstream | A or U |
| GenScript variants | eSp, eGFP, Nickase, WT | WT | WT |
| Compliance | Research GMP |
RUO | RUO |
Cas9 Nucleases and Nickases
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03701-500 |
GenCRISPR™ Cas9 v1.1 - 500 μg |
$704.00 |
|
Z03701-100 |
GenCRISPR™ Cas9 v1.1 - 100 μg |
$206.80 |
|
Z03701-1 |
GenCRISPR™ Cas9 v1.1 - 1 mg |
$1189.10 |
|
Z03702-1 |
GenCRISPR™ Cas9 v1.2 - 1 mg | - | |
Z03699-1 |
GenCRISPR™ SaCas9 2NLS Nuclease - 1 mg | - | |
Z03699-0.5 |
GenCRISPR™ SaCas9 2NLS Nuclease - 0.5 mg |
$705.00 |
|
Z03699-0.1 |
GenCRISPR™ SaCas9 2NLS Nuclease - 0.1 mg |
$210.00 |
|
Z03386-50 |
GenCrispr Cas9 Nuclease - 50 μg (5×10μg)(0.2mg/ml) |
$111.00 |
|
Z03386-10 |
GenCrispr Cas9 Nuclease - 10 μg (0.2mg/ml) |
$35.00 |
|
Z03385-50 |
GenCrispr Cas9-C-NLS Nuclease - 50 μg (1mg/ml) |
$146.00 |
|
Z03385-100 |
GenCrispr Cas9-C-NLS Nuclease - 100 μg (4mg/ml) |
$188.00 |
|
Z03388-50 |
GenCrispr Cas9-N-NLS Nuclease - 50 μg (1mg/ml) |
$146.00 |
|
Z03388-100 |
GenCrispr Cas9-N-NLS Nuclease - 100 μg (4mg/ml) |
$188.00 |
|
Z03468-500 |
GenCrispr Cas9-N-NLS Nuclease - 500 µg (10mg/ml) |
$640.00 |
|
Z03468-100 |
GenCrispr Cas9-N-NLS Nuclease - 100 µg (10mg/ml) |
$188.00 |
|
Z03468-1 |
GenCrispr Cas9-N-NLS Nuclease - 1 mg (10mg/ml) |
$1081.00 |
|
Z03389-50 |
GenCrispr NLS-Cas9-NLS Nuclease - 50 μg (1mg/ml) |
$146.00 |
|
Z03389-100 |
GenCrispr NLS-Cas9-NLS Nuclease - 100 μg (4mg/ml) |
$188.00 |
|
Z03469-500 |
GenCrispr NLS-Cas9-NLS Nuclease - 500 µg (10mg/ml) |
$640.00 |
|
Z03469-100 |
GenCrispr NLS-Cas9-NLS Nuclease - 100 µg (10mg/ml) |
$188.00 |
|
Z03469-1 |
GenCrispr NLS-Cas9-NLS Nuclease - 1 mg (10mg/ml) |
$1081.00 |
|
Z03390-50 |
GenCrispr NLS-Cas9-D10A Nickase - 50 μg (1mg/ml) |
$219.00 |
|
Z03390-100 |
GenCrispr NLS-Cas9-D10A Nickase - 100 μg (4mg/ml) |
$319.00 |
|
Z03390-10 |
GenCrispr NLS-Cas9-D10A Nickase - 10 µg (1mg/ml) |
$99.00 |
|
Z03393-50 |
GenCrispr NLS-Cas9-EGFP Nuclease - 50 μg (1mg/ml) |
$188.00 |
|
Z03393-100 |
GenCrispr NLS-Cas9-EGFP Nuclease - 100 µg (3mg/ml) |
$241.00 |
|
Z03467-500 |
GenCrispr NLS-Cas9-EGFP Nuclease - 500 μg(10mg/ml) |
$818.00 |
|
Z03467-100 |
GenCrispr NLS-Cas9-EGFP Nuclease - 100 μg(10mg/ml) |
$241.00 |
|
Z03467-1 |
GenCrispr NLS-Cas9-EGFP Nuclease - 1 mg(10mg/ml) |
$1375.00 |
|
Z03470-300 |
GenCrispr eSpCas9-N-NLS Nuclease - 300 µg (4mg/ml) |
$532.00 |
|
Z03470-100 |
GenCrispr eSpCas9-N-NLS Nuclease - 100 µg (4mg/ml) |
$240.00 |
Cas12a Nuclease
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03502-500 |
GenCRISPR™ Cas12a (Cpf1) Nuclease - 500 μg | - | |
Z03502-50 |
GenCRISPR™ Cas12a (Cpf1) Nuclease - 50 μg |
$105.00 |
|
Z03502-100 |
GenCRISPR™ Cas12a (Cpf1) Nuclease - 100 μg |
$169.00 |
|
Z03502-1 |
GenCRISPR™ Cas12a (Cpf1) Nuclease - 1 mg | - |
Cas13a Nuclease
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03486-500 |
GenCRISPR™ Cas13a (C2c2) Nuclease - 500 μg |
$785.00 |
|
Z03486-100 |
GenCRISPR™ Cas13a (C2c2) Nuclease - 100 μg |
$189.00 |
|
Z03486-1 |
GenCRISPR™ Cas13a (C2c2) Nuclease - 1 mg | - |
| Applications (DNA Editing) |
Nuclear localization signal (NLS) |
Biparticle nucleus localization signal (BPNLS) |
Nucleoplasmin nucleus localization signal (Nucleoplasmin NLS) |
|
|---|---|---|---|---|
| Nuclear localization and in vivo gene-editing | Double Strand Break |
|
GenCRISPR™ Cas9 v1.1 nuclease | |
| Single Strand Break |
|
GenCRISPR™ Cas9 v1.2 nuclease | ||
| FACS/Fluorescence |
|
|||
| Double-strand, staggered break |
|
|||
| In vitro target DNA cleavage | GenCRISPR™ Cas9 nuclease | |||
GenCRISPR™ Transfection reagent New!
Related Products
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03396-250 |
GenCrispr T7 Endonuclease I - 250 U (10000 U/ml) |
$55.00 |
|
Z03396-1250 |
GenCrispr T7 Endonuclease I - 1250 U (10000 U/ml) |
$220.00 |
|
L00694-20 |
GenCrispr sgRNA Synthesis Kit - 20 reactions |
$379.00 |
|
L00689-30 |
GenCrispr sgRNA Screening Kit - 30 reactions |
$166.00 |
|
L00688-25 |
GenCrispr Mutation Detection Kit - 25 reactions |
$180.00 |
To test one of our Cas9 enzymes, please Click Here.
Download a simple protocol for gene editing using GenCRISPR™ Cas9 nuclease
How to Order CRISPR/Cas9 products from GenScript
Have questions? GenScript's Ph.D.-level service representatives are available 24 hours a day, Monday through Friday, to assist you.
Enzyme activity validation guarantee
The GenCRISPR™ Gene editing technology has been licensed-in from the Broad Institute. The activity of all GenCRISPR™ Cas9 nucleases has been validated by in-vitro or in-vivo assays. The bioactivity data for all enzymes is included in the protocol document.
