Mammalian CHO Expression ^New

Mammalian CHO Expression Services

Transient expression in mammalian cells is crucial when post-translational modifications and appropriate protein folding are desired for downstream applications. CHO (Chinese Hamster Ovary) cells are the principal mammalian host used for recombinant protein and antibody production. The next generation proprietary CHO transient expression developed by GenScript significantly increases the yield and shortens the production time. This is a cost-effective solution which also enables small scale (microgram) to kilogram level protein production based on the same expression system for the best consistency and minimal surprises during the downstream steps.

Read to learn how CHO expressions can accelerate developing and manufacturing of therapeutic antibodies

Fill in this form to download the Nature advertorial for free

*

*

  

   The customer has downloaded our “Nature Advertorial Campaign for Next Gen CHO” and left his/her contact information below, he/she may interested in the Next Gen CHO services related Cat#: SC2806P, SC2806, SC3806P, SC3806, SC1946. Please contact the customer according to his/her comments or asking if he/she needs more assistance related to this services. Thanks! Next Gen CHO Product page: https://www.genscript.com/transient-expression-overview.html

• 

  Higher Yield

  Reach 2+g/L for transient expression

• 

  Shorter TAT

  Production time includes gene synthesis

• 

  Superior Quality

  Full range QC and characterizations

• 

  Cost-Effectiveness

  Taking advantage of our rich experience

• 

  0 Error Rate

  Unique QR code tracking and automation

• 

  Validated Consistency

  Scale-up in the same CHO cell line

Selection Guide

Start and finish in the same CHO expression system: From microgram to kilogram level production.

	CHO-HT (High Throughput)	CHO-Express	CHO-HP
Requested Protein Amount	Expression Screening or Protein Delivery (Target amount* 0.1-5 mg)	500 mg or less	500 mg to kilograms
Production Time Starting from (Gene Synthesis Included)	2 weeks	3 weeks	6 weeks
Average Yield (Antibody)	300 mg/L	400 mg/L	1.5 g/L
Deliverables	Purity: ≥90% Endotoxin control*: ≤1EU/mg	Purity: ≥95% for antibody; ≥90% for protein Endotoxin level: ≤1EU/mg Conc. ≥1.0mg/mL
Default QC	SDS-PAGE, A280	SDS-PAGE, SEC-HPLC, endotoxin
Additional QC and Characterizations	Endotoxin*, LC-MS* SEC-HPLC	LC-MS
	Affinity test, cross-reactivity, epitope mapping, in vitro and in vivo efficacy, stability test by freeze-thaw cycles, sterility test, and more
Purification	Standard 1-step purification (Optional*: 2- or 3-steps)	Multiple purification steps included
Quotation and Ordering	HT Request Form Request Now	Get A Quote NowRequest Now

Notes: Read More »

• This customized solution accepts all types of proteins, antibody species and isotypes, fragments.
• All incoming projects are subjected to evaluation.
• Additional QC and Characterizations are offered with extra charge upon request.
• Production time include gene synthesis.

High Throughput (CHO-HT) production

• * Target amount (0.1-5 mg) is based on common isotypes of full length IgG, not guaranteed.
• * 2 weeks production time is based on antibody and antibody fragments.
• * Random selection of 5% of samples to go through endotoxin and LC-MS analysis. Testing for all samples can be requested with extra charge.
• * Extra step purification is offered upon request, by default one-step purification based on protein types.
• * 24+ samples required to order High Throughput CHO-HT service.

CHO-Express and CHO-HP production

• Pilot expression evaluation can be requested for CHO-HP only.

Case Studies

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

•  
  Email: protein@genscript.com
•  
  Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
•  
  Fax: 1-732-210-0262