taq_dna_polymerase
Taq DNA Polymerase
GenScript Taq DNA Polymerases are highly thermostable recombinant DNA polymerases and ideally suited for routine PCR reactions. The enzyme has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at 3' end of the extension product. In addition, GenScript Taq DNA Polymerase is the ideal tool for routine PCR of templates 8 kb from lambda DNA.
We also provide PCR Master Mix which is a premixed 2X concentrated solution of Taq DNA Polymerase. The mixture is optimized for consistent and efficient routine PCR, it contains all components for PCR, except DNA template and primers.
Highlights
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Applications
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Ordering Information
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
E00007-50000 |
Taq DNA Polymerase - 50000 U (1000.0U/vial) |
$2970.00 |
|
E00007-1000 |
Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
|
E00008 |
Taq DNA Polymerase without Mg2+ - 1000 U (1000.0U/vial) |
$66.00 |
|
E00012 |
Taq DNA Polymerase, concentrated - 2500 U (2500.0U/vial) |
$164.00 |
|
E00019 |
2X Taq Master Mix - 100 Reactions (2.5 mL) |
$48.00 |
|
E00101 |
Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml) - 1 PK |
$86.00 |
*These products are also aviaiable in larger package sizes or customized requests, please quote
| Storage Condition | Stable at -20°C for one year. Note: The enzyme can be stable at room temperature or even 37°C for seven days without any loss of activity. |
|---|---|
| Concentration | 5 U/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol. |
| Recombinant (Yes/No) | Yes |
| Product Source | E. coli cells |
| Definition of Activity Unit | One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. |
| 10 X Reaction Buffer (with Mg2+) |
500 mM KCl, 100 mM Tris HCl (pH9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. |
| Hazardous | No |
| 5' - 3' Exonuclease | Yes |
| 3' - 5' Exonuclease | No |
| RNases contamination | No |
| Error Rate | NA |
PCR performance, activity, nuclease
Lane |
Taq |
Unit |
|---|---|---|
1 |
Leading
Brand A |
0.1 |
2 |
0.25 |
|
3 |
0.5 |
|
4 |
Leading
Brand B |
0.1 |
5 |
0.25 |
|
6 |
0.5 |
|
7 |
GenScript |
0.1 |
8 |
0.25 |
|
9 |
0.5 |
FAQs »
- What is the recommended amount of Taq DNA Polymerase or Green Taq DNA Polymerase to use?
- How is Taq different from Green Taq?
- Can Taq DNA Polymerase or Green taq be used to amplify GC-rich amplicons?
- Can Taq DNA Polymerase be used in other buffers?
- Which buffer should I use if I want to control the level of magnesium in the reaction? Or does the presence of Mg2+ inhibit PCR?
- How should I set up an amplification reaction using Taq DNA Polymerase?
- What is the recommended concentration of Taq Polymerase to use for a routine PCR reaction?
- What is the upper limit for length of the product when using Taq DNA Polymerase from GenScript?
- What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
- Why is there no product when visualized on an agarose gel?
- If the product sequence doesn't completely match the expected sequence. How can this result be improved?
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
- Where can I find help troubleshooting my PCR?
Answer:
- What is the recommended amount of Taq DNA Polymerase or Green Taq DNA Polymerase to use?
- How is Taq different from Green Taq?
- Can Taq DNA Polymerase or Green taq be used to amplify GC-rich amplicons?
- Can Taq DNA Polymerase be used in other buffers?
- Which buffer should I use if I want to control the level of magnesium in the reaction? Or does the presence of Mg2+ inhibit PCR?
- How should I set up an amplification reaction using Taq DNA Polymerase?
- 0.5 μl Taq DNA Polymerase
- 1 μl 10 mM dNTP
- 1 μl each primer
- 2 μl genomic template (up to 100 ng/μl)
- 5 μl 10 X Taq Buffer (Standard Taq containing Mg2+)
- 39.5 μl sterile or filtered H2O
- Denature at 94°C
- Extend 1 minute/kb
- What is the recommended concentration of Taq Polymerase to use for a routine PCR reaction??
- For up to 3 kb, we recommend 1.25 units per 50 µl reaction.
- For specialized applications, including 3–8 kb amplicons, 2.5 units per 50 µl reaction is recommended.
- What is the upper limit for length of the product when using Taq DNA Polymerase from GenScript?
- What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
- Why is there no product when visualized on an agarose gel?
- How can I improve my results if the product sequence doesn't completely match the expected sequence?
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
- Where can I find help troubleshooting my PCR?
A: .5 U or more.
A: The Green Taq contains Taq polymerase with a special stabilizer that allows it to be stable at room temperature for 2 weeks.
A: No.
A: The Taq DNA polymerase is 100% active in the buffer supplied by GenScript, but also works in competitors' buffers. For your convenience, we sell 10 x Taq buffer Cat. No. B0005.
A: We suggest using Mg-free Taq DNA Polymerase (Cat. No. E00008).
A: Here are the guidelines for 50 μl PCR reaction:
A:
A: Up to λdna 8 kb.
A: The Poly A tail contains sticky ends, and can only do TA cloning.
A: There may be a problem in the PCR program or system, or a problem during the electrophoresis.
A: We suggest to use a high-fidelity DNA Polymerase which can offer the highest fidelity of any commercial thermostable polymerase.
A: The Taq can be used when the PCR reaction started or after degeneration
A: No. The exonuclease will only degrade double stranded DNA that it encounters while extending a DNA fragment. It will degrade a secondary primer if bound to the same strand (e.g. a mutagenesis primer).
A: Please send all your issue via product@genscript.com, or load the protocol of the Taq DNA polymerase direcly.
Datasheets »
Protocol »
MSDS »
Citations »
- Wang X., et al. Salicylic Acid Regulates Plasmodesmata Closure during Innate Immune Responses in Arabidopsis. Plant Cell.2013 Jun;25(6):2315-29.
- Ruth B. Phillips., et al. Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome. Int J Genomics.2013 Mar;261730.
- FrÍas M., et al. The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif in the protein surface. Mol Plant Pathol.2013 Qct;12097.
- Y Acanda., et al. EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine. Plant Cell Rep.2013 Dec.
- Firlej A., et al. A Multi-Approach Study to Delineate Interactions Between Carabid Beetles and Soybean Aphids. Environ Entomol.2013 Feb;42(1):89 - 96.
- Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol.2013 Mar;27(3):511 - 523.
- Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One.2013 Jan;8(1):e50627.
- Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A.2013 Mar;110(10):3943 - 3948.
- Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One.2013 May;6(5):e62394.
- Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb).2013 Jul;111(1):16-22.
- Carol D. von Dohlen., et al. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid (Adelges tsugae)(Hemiptera: Adelgidae) from its native and introduced range. Environ Microbiol.2013 Jul;15(7):2043-62.
- Gandhirajan RK., et al. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation. J Clin Invest.2013 Feb;123(2):887-902.
- Zhou JB., et al. Identification of a novel gene fusion RNF213â€'SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep.2013 Feb;7(2):591-7.
- Jessica Hoppstdter., et al.Glucocorticoid-induced leucine zipper is downregulated in human alveolar macrophages upon Toll-like receptor activation. Eur J Immunol.2012 May;42(5):1282-93.
- Sheng H., et al. Mapping QTL for resistance to eyespot of wheat in Aegilops longissima. Theor Appl Genet.2012 Jul;125(2):355-366.
Following PCR related products are also available in larger package sizes.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
E00043 |
Green Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
|
A01849-250 |
Hot Start Taq Antibody - 250 U (5U/μl) |
$126.00 |
|
A01849-1000 |
Hot Start Taq Antibody - 1000 U (5U/μl) |
$399.00 |
|
L00342 |
High-Stability PCR Kit - 1 kit |
$68.00 |
|
C01689 |
Stabilized dNTP Mix, 10 mM each - 0.5 ml |
$34.00 |
|
D0056 |
dNTP mixture, 10 mM each - 0.5 ml |
$29.00 |
|
C01577-50 |
dATP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01577-250 |
dATP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01577-10 |
dATP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01577-1 |
dATP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01578-50 |
dGTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01578-250 |
dGTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01578-10 |
dGTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01578-1 |
dGTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01579-50 |
dCTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01579-250 |
dCTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01579-10 |
dCTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01579-1 |
dCTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01580-50 |
dTTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01580-250 |
dTTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01580-10 |
dTTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01580-1 |
dTTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01582-50 |
dNTP (10 mM each) ≥99% HPLC - 50 ml (50ml/bottle) |
$828.00 |
|
C01582-250 |
dNTP (10 mM each) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$2875.00 |
|
C01582-10 |
dNTP (10 mM each) ≥99% HPLC - 10 ml (10.0ml/vial) |
$178.00 |
|
C01582-1 |
dNTP (10 mM each) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
