Stable Cell Line Development Services For Recombinant Protein & Antibody Production
Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. At GenScript, we offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript's top quality stable cell line services.
For Assay Development Stable Cell Lines Service, please click here.
GenScript Production Stable Cell Line Service Options
- Host cells: CHO, CHO-K1, Glutamine Synthetase-knockout CHO-K1, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, U-2OS, or customer-specified cell line.
- Genes: Antibodies, GPCRs, ion channels, proteases, kinases, transmembrane proteins and more.
- Customized Service: Choose part or all of the following steps based on your specific needs.
Stable Cell Line Service Details
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Steps
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Gene synthesis & subcloning (optional)
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Transient expression evaluation
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Stable cell line development
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Process development
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Deliverables
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Target gene(s) in pUC57 & vector map
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Expression report
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Top 3 stable clones
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Comprehensive report
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Description
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Guaranteed
Gram Level (SC1709) |
Stable
Bioproduction (SC1503) |
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Features
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Selection system
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Glutamine Synthetase or DHFR
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Guaranteed yield
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-
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Steps
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Gene synthesis & subcloning(optional) |
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V
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Transient expression evaluation
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V
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V
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Stable cell line development
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V
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V
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Process development
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optional
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optional
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Production service
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optional
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optional
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Deliverables
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Target gene(s) in pUC57 & vector map
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V
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V
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Expression Report
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V
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Top 3 stable clones
(5 clones for SC1557) |
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V
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Comprehensive report
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Turnaround time
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weeks
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14-17
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26-29
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Price
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We offer GLP compliant services with our packages.
Flow Chart Of GenScript Stable Transfection & Cell Line Development Service
High quality cell lines start with high quality DNA
Our customers have the option of providing their own DNA for stable cell line generation purposes, however, GenScript recommends gene synthesis to ensure that we use high quality.
Stable transfection
GenScript allows you to choose from either Glutamine Synthetase or DHFR gene expression systems to construct your stable cell line. To start your project, DNA carrying the gene of interest must be introduced into the cell with a stable transfection protocol. Depending on your preference, we will use either plasmid-based stable transfection or lentivirus-based infection as your preferred stable transfection method.
Transient expression evaluation
To ensure that your protein of interest is expressed in the cells, we first characterize your protein expression by ELISA or Western Blot 48 hours after the transfection.
Stable cell line development
Transfected cells are grown in the appropriate selective media to generate stable pools. For packages using a DHFR expression system, we can also subject the stable pools to additional Methotrexate (MTX) amplification and screening, in order to increase expression levels (packages using a Glutamine Synthetase expression system do not require gene amplification). The productivity and quality of the pools is evaluated up to 10 passages for the top 10 clones.
Clone picking
Top 3 clones from each pool are picked and evaluated further, based on their growth rates.
Cell line stability screening
Stability testing is a critical component of characterizing bioproduction cell lines. Stable cell lines in continuous culture suffer from genetic instability as the number of passages increases. Stability test will help you ensure the quality of your production cells over the intended passages.
Final deliverables, QC data and full report
We provide the sequence and construct report (in case of synthesized gene), top 3 stable clones and a comprehensive stable cell line generation report
For any other inquiries, please contact us using the details listed below. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
