Routine PCR Reagents
GenScript's advanced technology has led to the invention of high-quality PCR reagents, including the recombinant form of regular Taq DNA Polymerases, high-stability Taq DNA Polymerases, dNTPs, Master Mixes, and more. They simply provide you with instant access to high throughput PCR automation, offering cost-effective solution and satisfactory results.
Applications
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Taq DNA Polymerase
GenScript's best-selling Taq DNA Polymerase is a cost-effective recombinant enzyme.Taq DNA Ploymerase consists of a single polypeptide with a molecular weight of 94 kDa.Taq is the most common polymerase used for routine PCR.
Ordering Information
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
E00049-250 |
Hot Start Taq DNA Polymerase - 250 U |
$130.00 |
|
E00049-1000 |
Hot Start Taq DNA Polymerase - 1000 U |
$490.00 |
|
E00007-50000 |
Taq DNA Polymerase - 50000 U (1000.0U/vial) |
$2970.00 |
|
E00007-1000 |
Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
|
E00043 |
Green Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
|
E00008 |
Taq DNA Polymerase without Mg2+ - 1000 U (1000.0U/vial) |
$66.00 |
|
E00012 |
Taq DNA Polymerase, concentrated - 2500 U (2500.0U/vial) |
$164.00 |
|
E00101 |
Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml) - 1 PK |
$86.00 |
|
L00342 |
High-Stability PCR Kit - 1 kit |
$68.00 |
|
E00019 |
2X Taq Master Mix - 100 Reactions (2.5 mL) |
$48.00 |
|
E00050-40000 |
M-MuLV Reverse Transcriptase - 40000 U |
$189.00 |
|
E00050-10000 |
M-MuLV Reverse Transcriptase - 10000 U |
$58.00 |
*These products are also available in larger package sizes.
Free samples available.
| Concentration |
5 units/μl
|
|
|---|---|---|
| Recombiant Enzyme |
Yes
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| Format | Supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately | |
| Contaminating nucleases |
No
|
|
| Contaminating RNases |
No
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|
| Contaminating proteases |
No
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Specification of Taq DNA Polymerase
QC Tests
PCR* performance, activity, nuclease
Lane 1-3: Leadig Brand A
Lane 4-6: Leading Brand B
Lane 7-9: GenScriptTaq DNA Polymerase
Examples
A 500 bp lamda DNA fragment was amplified with Taq DNA Polymerase from different brands
FAQs »
- What is the recommended amount of Taq DNA Polymerase or Green Taq DNA Polymerase to use?
- How is Taq different from Green Taq?
- Can Taq DNA Polymerase or Green taq be used to amplify GC-rich amplicons?
- Can Taq DNA Polymerase be used in other buffers?
- Which buffer should I use if I want to control the level of magnesium in the reaction? Or does the presence of Mg2+ inhibit PCR?
- How should I set up an amplification reaction using Taq DNA Polymerase?
- What is the recommended concentration of Taq Polymerase to use for a routine PCR reaction?
- What is the upper limit for length of the product when using Taq DNA Polymerase from GenScript?
- What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
- Why is there no product when visualized on an agarose gel?
- If the product sequence doesn't completely match the expected sequence. How can this result be improved?
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
- Where can I find help troubleshooting my PCR?
- What is the recommended amount of Taq DNA Polymerase or Green Taq DNA Polymerase to use?
- How is Taq different from Green Taq?
- Can Taq DNA Polymerase or Green taq be used to amplify GC-rich amplicons?
- Can Taq DNA Polymerase be used in other buffers?
- Which buffer should I use if I want to control the level of magnesium in the reaction? Or does the presence of Mg2+ inhibit PCR?
- How should I set up an amplification reaction using Taq DNA Polymerase?
- 0.5 μl Taq DNA Polymerase
- 1 μl 10 mM dNTP
- 1 μl each primer
- 2 μl genomic template (up to 100 ng/μl)
- 5 μl 10 X Taq Buffer (Standard Taq containing Mg2+)
- 39.5 μl sterile or filtered H2O
- Denature at 94°C
- Extend 1 minute/kb
- What is the recommended concentration of Taq Polymerase to use for a routine PCR reaction??
- For up to 3 kb, we recommend 1.25 units per 50 µl reaction.
- For specialized applications, including 3–8 kb amplicons, 2.5 units per 50 µl reaction is recommended.
- What is the upper limit for length of the product when using Taq DNA Polymerase from GenScript?
- What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
- Why is there no product when visualized on an agarose gel?
- How can I improve my results if the product sequence doesn't completely match the expected sequence?
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
- Where can I find help troubleshooting my PCR?
A: .5 U or more.
A: The Green Taq contains Taq polymerase with a special stabilizer that allows it to be stable at room temperature for 2 weeks.
A: No.
A: The Taq DNA polymerase is 100% active in the buffer supplied by GenScript, but also works in competitors' buffers. For your convenience, we sell 10 x Taq buffer Cat. No. B0005.
A: We suggest using Mg-free Taq DNA Polymerase (Cat. No. E00008).
A: Here are the guidelines for 50 μl PCR reaction:
A:
A: Up to λdna 8 kb.
A: The Poly A tail contains sticky ends, and can only do TA cloning.
A: There may be a problem in the PCR program or system, or a problem during the electrophoresis.
A: We suggest to use a high-fidelity DNA Polymerase which can offer the highest fidelity of any commercial thermostable polymerase.
A: The Taq can be used when the PCR reaction started or after degeneration.
A: No. The exonuclease will only degrade double stranded DNA that it encounters while extending a DNA fragment. It will degrade a secondary primer if bound to the same strand (e.g. a mutagenesis primer).
A: Please send all your issue via product@genscript.com, or load the protocol of the Taq DNA polymerase direcly.
Citations »
- Wang X., et al. Salicylic Acid Regulates Plasmodesmata Closure during Innate Immune Responses in Arabidopsis. Plant Cell.2013 Jun;25(6):2315-29.
- Ruth B. Phillips., et al. Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome. Int J Genomics.2013 Mar;261730.
- FrÍas M., et al. The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif in the protein surface. Mol Plant Pathol.2013 Qct;12097.
- Y Acanda., et al. EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine. Plant Cell Rep.2013 Dec.
- Firlej A., et al. A Multi-Approach Study to Delineate Interactions Between Carabid Beetles and Soybean Aphids. Environ Entomol.2013 Feb;42(1):89 - 96.
- Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol.2013 Mar;27(3):511 - 523.
- Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One.2013 Jan;8(1):e50627.
- Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A.2013 Mar;110(10):3943 - 3948.
- Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One.2013 May;6(5):e62394.
- Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb).2013 Jul;111(1):16-22.
- Carol D. von Dohlen., et al. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid (Adelges tsugae)(Hemiptera: Adelgidae) from its native and introduced range. Environ Microbiol.2013 Jul;15(7):2043-62.
- Gandhirajan RK., et al. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation. J Clin Invest.2013 Feb;123(2):887-902.
- Zhou JB., et al. Identification of a novel gene fusion RNF213â€'SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep.2013 Feb;7(2):591-7.
- Jessica Hoppstdter., et al.Glucocorticoid-induced leucine zipper is downregulated in human alveolar macrophages upon Toll-like receptor activation. Eur J Immunol.2012 May;42(5):1282-93.
- Sheng H., et al. Mapping QTL for resistance to eyespot of wheat in Aegilops longissima. Theor Appl Genet.2012 Jul;125(2):355-366.
Brochure »
Molecular Biology Products
Simplify your molecular biology workflow with GenScript's molecular biology solutions.
- PCR reagents
- Cloning kits
- Vectors
- dNTPs
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01849-250 |
Hot Start Taq Antibody - 250 U (5U/μl) |
$126.00 |
|
A01849-1000 |
Hot Start Taq Antibody - 1000 U (5U/μl) |
$399.00 |
|
D0056 |
dNTP mixture, 10 mM each - 0.5 ml |
$29.00 |
|
C01689 |
Stabilized dNTP Mix, 10 mM each - 0.5 ml |
$34.00 |
|
C01577-50 |
dATP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01577-250 |
dATP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01577-10 |
dATP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01577-1 |
dATP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01578-50 |
dGTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01578-250 |
dGTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01578-10 |
dGTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01578-1 |
dGTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01579-50 |
dCTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01579-250 |
dCTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01579-10 |
dCTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01579-1 |
dCTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01580-50 |
dTTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01580-250 |
dTTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01580-10 |
dTTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01580-1 |
dTTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01581-50 |
dNTP (25 mM each) ≥99% HPLC - 50 ml (50ml/bottle) |
$1600.00 |
|
C01581-250 |
dNTP (25 mM each) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$7500.00 |
|
C01581-10 |
dNTP (25 mM each) ≥99% HPLC - 10 ml (10.0ml/vial) |
$360.00 |
|
C01581-1 |
dNTP (25 mM each) ≥99% HPLC - 1 ml(1.0ml/vial) |
$45.00 |
|
C01582-50 |
dNTP (10 mM each) ≥99% HPLC - 50 ml (50ml/bottle) |
$828.00 |
|
C01582-250 |
dNTP (10 mM each) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$2875.00 |
|
C01582-10 |
dNTP (10 mM each) ≥99% HPLC - 10 ml (10.0ml/vial) |
$178.00 |
|
C01582-1 |
dNTP (10 mM each) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
