GenScript oligonucleotide codes
GenScript oligonucleotide codes
GenScript provides convenient online instant quotation and ordering system to build your desired oligonucleotides for comprehensive applications. It is an integrated system to easily build almost all type of single-stranded oligos, such as DNA and RNA oligos with modification and labeling (dual and multiple labeling), antisense oligonucleotides (phosphorothioate DNA and RNA, 2'-OMe-RNA, 2'-F-RNA), chimeric oligonucleotides, etc.
To best use our online instant quotation and ordering system, please use GenScript defined codes as following:
1. Backbones
| Backbone | Valid Characters | Validated Code |
|---|---|---|
| DNA | A, C, G, T | (A), (C), (G), (T) |
| Phosphorothioated DNA | A, C, G, T | (A*), (C*), (G*), (T*) |
| RNA | A, C, G, U | (rA), (rC), (rG), (rU) |
| Phosphorothioated RNA | A, C, G, U | (rA*), (rC*), (rG*), (rU*) |
| 2'-OMe-RNA | A, C, G, U | (mA), (mC), (mG), (mU) |
| Phosphorothioated 2'-OMe-RNA | A, C, G, U | (mA*), (mC*), (mG*), (mU*) |
2. Chimeric bases
| Backbone | Chimeric bases | Insert Code |
|---|---|---|
| DNA | A, C, G, T | (dA), (dC), (dG), (dT) |
| Phosphorothioated DNA | A, C, G, T | (dA*), (dC*), (dG*), (dT*) |
| RNA | A, C, G, U | (rA), (rC), (rG), (rU) |
| Phosphorothioated RNA | A, C, G, U | (rA*), (rC*), (rG*), (rU*) |
| 2'-OMe-RNA | A, C, G, U | (mA), (mC), (mG), (mU) |
| Phosphorothioated 2'-OMe-RNA | A, C, G, U | (mA*), (mC*), (mG*), (mU*) |
| 2'-F-RNA | A, C, G, U | (fA), (fC), (fG), (fU) |
3. Standard Mixed DNA Bases:
| Mixed DNA Bases | Insert Code |
|---|---|
| dA+dG+dC+dT | (N) |
| dT+dC+dG | (B) |
| dA+dT+dG | (D) |
| dA+dT+dC | (H) |
| dA+dC+dG | (V) |
| dA+dC | (M) |
| dA+dG | (R) |
| dA+dT | (W) |
| dC+dG | (S) |
| dC+dT | (Y) |
| dT+dG | (K) |
4. Custom Mixed DNA Bases:
| Mixed DNA Bases | Insert Code |
|---|---|
| X1%dA+X2%dC+X3%dG+X4%dT | (X1%AX2%CX3%GX4%T) |
| e.g. 30%dA+30%dC+20%dG+20%dT | (30%A30%C20%G20%T) |
5. 5', internal and 3' modification/labeling:
| Classification | Modification | 5' Mod Code | Internal Mod Code | 3' Mod Code |
|---|---|---|---|---|
Fluorescent labeling | CY3 | // | // | // |
| CY5 | // | // | // | |
| FAM | // | // | // | |
| HEX | // | // | // | |
| 6JOE | // | // | // | |
| ROX | // | // | // | |
| TAMRA | // | // | // | |
| TET | // | // | // | |
Quenchers | DABCYL | - | - | // |
| BHQ 1 | - | - | // | |
| BHQ 2 | - | - | // | |
| ECLIPSE | - | - | // | |
Non-Fluorescent conjugates | Biotin | // | // | // |
| Biotin TEG | // | // | // | |
| Dual Biotin | // | // | - | |
| Biotin dT | // | // | - | |
| PC Biotin | // | // | - | |
| Digoxin | // | // | // | |
Terminal Phosphorylation | Phosphorylation | // | N/A | // |
Linkers | Amino Linker C3 | // | // | // |
| Amino Linker C6 | // | // | // | |
| Amino Linker C7 | // | // | // | |
| Amino Linker C12 | // | // | // | |
| Amino Linker C6 dT | // | // | // | |
| Disulfide Linker C3 | // | // | // | |
| Disulfide Linker C6 | // | // | // | |
Spacers | Spacer C3 | // | // | // |
| Spacer C6 | // | // | // | |
| Spacer 9 | // | // | // | |
| Spacer 18 | // | // | // | |
| dSpacer | // | // | // | |
Modified bases | DeoxyInosine | // | // | // |
| DeoxyUridine | // | // | // | |
| 2'-Fluoro A | // | // | // | |
| 2'-Fluoro C | // | // | // | |
| 2'-Fluoro G | // | // | // | |
| 2'-Fluoro U | // | // | // | |
Electrochemicals | Ferrocene | // | // | // |
