Preclinical Plasmid Preparation Service
GenScript is providing Preclinical Plasmid Preparation service for you to fix the problems existing in animal trials. Adopting relevant features of GMP manufacturing, including process, environment and materials segregation, preclinical plasmid DNA can be used as a raw material for producing viral candidates used in animal trials. Flexible quantity options from microgram level to gram level are available to satisfy your needs.
What can you expect from our service?
✔ Animal-free
✔ Antibiotic-freeLow risk to animal health
✔ High yield
✔ High-efficiency process (3-4 weeks)Save your time and your money
✔ Free of impurities (HCD, HCR)
✔ Higher active ingredient content (supercoil) > 90%Guarantee the consistence and reproducibility of your trials
Good Start! – Time Limited Promotion
Order 2 pieces, get the cheaper one for free!
Application Terms:
- Promotion is valid for domestic (US) and CN customers.
- Promotion is valid for Preclinical Plasmid Preparation service in catalog no. SC2019.
- Promotion is effective only for orders placed on from Apr 1st, 2019 to May 31st, 2019.
- Promotion code PrePlasBOGOF will be auto applied at checkout and may not be applied to orders after they have been confirmed.
- Only one promotion code can be used for each order. This promotion cannot be combined with any other promotion or special pricing agreement.
| Quantity | Deliverable | Turnaround Time (week) | Price |
|---|---|---|---|
| 5 mg |
|
2-3 | Quote |
| 10 mg | |||
| 20 mg | |||
| 50 mg | |||
| 100 mg | |||
| 200 mg | |||
| 500 mg | |||
| Gram level | Quote |
| QC Item | QC Method | Release Standard |
|---|---|---|
| Appearance | Visual inspection | Colorless, clear, free of precipitate or foreign particles |
| A260/A280 | Absorbance ratio | 1.8-2.0 |
| Restriction Digest | AGE | Pass |
| Supercoiled Content | HPLC | > 90% |
| Residual E. coli DNA | AGE | undetectable (gel) |
| Residual E. coli RNA | AGE | undetectable (gel) |
| Sequencing | Sanger | Match the expected |
| Endotoxin | LAL assay | < 10 EU/mg |
Fermentation Process
- High density fermentation
- High yield
Purification Process—2-step chromatography process
1. GF Elution—Efficient removal of RNA
Agarose gel electrophoresis (AGE): obvious decrease of RNA content through GF elution.
- M1: Supercoiled DNA Ladder Marker
- M2: 1 kb DNA Ladder Marker
- 1: Lysate
- 2: Sample after GF elution
- 3: Waste of salt elution
- 4: Waste of salt elution
- 5: Waste of water elution
- R: RNA bands
2. HIC Elution—High content of supercoiled plasmid
- Solvent
- Open circular plasmid
- Supercoiled plasmid
- Dimer plasmid
- Solvent
- Open circular plasmid
- Supercoiled plasmid
- Dimer plasmid
3. AEX Purification—Efficient removal of endotoxin
QC Item QC Method Release Standard Endotoxin LAL assay < 10 EU/mg
| Sample | Time | Area (%) | |
|---|---|---|---|
| GF-Elution | OC-Plasmid | 20.774 | 8.02 |
| SC-Plasmid | 21.723 | 83.17 | |
| dimer-Plasmid | 22.408 | 7.47 |
High Performance Liquid Chromatography (HPLC): supercoiled content reached around 83% after GF elution.
| Sample | Time | Area (%) | |
|---|---|---|---|
| HIC-Elution | OC-Plasmid | 20.800 | 1.62 |
| SC-Plasmid | 21.790 | 94.07 | |
| dimer-Plasmid | 22.533 | 4.31 |
High Performance Liquid Chromatography (HPLC): supercoiled content reached around 95% after HIC elution.
Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
