Endotoxin Detection & Removal System

ToxinEraser Endotoxin Removal System

• High binding capacity at least 2, 000, 000 EU/ml (CV).
• High Recovery Yield: >90% with minimized sample loss.
• High stability and high removal efficiency: Bring down endotoxin level to as low as 0.1 EU/ml^*.

^* With repeat use of ToxinEraser endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

GenScript ToxinEraser is an efficient endototoxin removal tool. The final endotoxin level can be reduced below 1 EU/mg with repeated use of ToxinEraser Advanced Endotoxin Removal resin. These kits can be used to remove endotoxin from proteins, peptides, antibodies, or even DNA samples.

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1. How to run an endotoxin detection assay for medical devices?

   Infuse 15 ml of endotoxin-free water into the lumen of medical device (e.g. infusion apparatus, dialysis tubing, etc.) and shocks five times, seal all the ends and incubate it at 37°C water bath for 2 hours, then transfer the water to an endotoxin-free vial. Detect the endotoxin concentration in the water by using ToxinSensor Chromogenic LAL Endotoxin Assay Kit(Cat. No. L00350), and the total endotoxin value in the water can be determined.

2. Can I use plastic ware when I prepare standard endotoxin solution?

   Endotoxins adhere strongly to glassware and are difficult to remove completely during washing. Standard laboratory autoclaving procedures have little or no effect on endotoxin levels. Heating glassware at 180°C overnight is recommended to destroy any attached endotoxin molecules. We recommend you to only use new plastic ware tubes which are certified to be endotoxin-free when you prepare standard endotoxin solution. GenScript supplies ToxinSensor Endotoxin-free Tubes (Cat. No. M01072) for you to dilute or aliquot samples. Endotoxin adheres to plastic surfaces more strongly than glass surfaces.

3. Is standard endotoxin solution reusable after developed (color formed)?

   No. Standard endotoxin solution should be prepared fresh for each test.

4. How to determine the reliability of the final data?

   Firstly, we recommend you to prepare all materials included in the kit in a laminar flow cabinet and avoid contamination during assay process.
   For ToxinSensor Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350), if the OD value of your sample is in the range of the standards, the final data will be reliable after you obtain a good linearity (R≥0.980).
   For ToxinSensor Gel Clot Endotoxin Assay Kit (Cat. No. L00351), you can obtain the reliable final result through negative and positive controls. If you get a positive result in the negative control, it may indicate that the LAL or LAL reagent water has been contaminated, or if you get a negative result in the positive control, it may indicate that the LAL reagent has lost activity.

5. Can I use 96-well microplate to read the absorbance of each reaction at 545 nm?

   ToxinSensor Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350) is designed to deliver high sensitivity quantitative assay in tubes, and not intended to be used for a 96-well plate assay. However, you can perform the assay in tubes, after color development, transfer 200 µl of the final solution into an endotoxin-free 96-well plate to read the result.

Read more

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