FREM2 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM2
Entrez Gene ID	101101900
Full Name	FRAS1 related extracellular matrix protein 2
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027973602.1	XP_027829403.1	FRAS1-related extracellular matrix protein 2
XM_004012101.3	XP_004012150.1	FRAS1-related extracellular matrix protein 2 isoform X1
XM_012130745.2	XP_011986135.1	FRAS1-related extracellular matrix protein 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv14026	XM_012130745.2 Latest version!	Ovis aries musimon FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm09286	XM_004012101.3 Latest version!	Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm59409	XM_027973602.1 Latest version!	Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM2 ( XM_012130745.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011986135.1 Ovis aries musimon FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv14026
Clone ID Related Accession (Same CDS sequence)	XM_012130745.2
Accession Version	XM_012130745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	FRAS1-related extracellular matrix protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCCCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCCCGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT TGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG CGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCAAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCCTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGTGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGCGG TAACACTATC CAAGGAGTTA CTGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAC 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACC CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCGCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTCCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTAACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACACT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AACGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CCAGTCTGAA TATTCCATTG AAGAGGACAT TGGCGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAAGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TCTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCA TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CGATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTA TGGCGGAGCG GCACTGACCT GTCCCAGGCT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACGT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAGTATT CGATGGCATA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCTG GACCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTGTCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACGTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986135.1
CDS	35..9553
Translation	MRATGTPGVTERTRPAGNPTKSPPTPPTPPGLVLLLLLSLVSRVRAQPPDPGRAVLSPDLAGVAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGAGEVRYSHLGARSPSRDRVRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLGALPRYGELLHYPQVPGPASKGDTQEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVAELRSRGSPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESAPDLLIFNLTSPFQPGQGFLVSTDDRSLPLSSFTQRDLHLLKIAYQPPSEDSDQERLFELELEVVDPEGAASDPFSFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVQLEVVAGLRHGQLVLLGASSGNSAPKTFTVAELAAGRVVYHHDDKDGSLSDNLILQMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPQALSATDMDSEDSLLLFVLEAPSSTAGHLLLRQTHPPQGEEMLLGSPWRKQGVFYEKTVTEWRQRDITEGRLFYRHSGPHSPGPVIDQFTFRVQDNHDPPNKSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPMDTDENHSPAPLGTLVLTDDPSVVVAHFTQAQVNHHKIAYRPPDQELGVAARVAQFQFQVEDQAGNVAPGTFTFYLQPVDNQPPEILNTGFTVQEKSHHILSETELGVNDVDTDVAHITFTLIQVPKHGHMKLSGQVLHVGGIFHLEDIKQGRISYAHNEAESLTDSCSLEVSDGHHVVPITLRVNVRPMDGELPVLSLPAGTLGSYLDVLENGATEITANVIRGTNGDADDLMLTFLLEDAPLYGEILVNGVPAEQFTQRDILEGSVVYSHTSGEIGLLPKEDSFNLSLSDMSQEWRIGGNTIQGVTVWVTILPVDSQAPEISVGEQFIVLEGDKSVITSMHISAEDIDSLNDDILCTIIIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIVIIPTNDEQPEIFIREFMVMEGMSLVVDTPILNAADADVPADDLTFTITKVPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGRHSVEKTVLIMVIPVDDETPRMAINNGLEIEIGETKIINNRILMATDLDSEDKFLVYIIRYGPGHGLLQRQTPTGVFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSIGSPDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLESTDQPRVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGEKKLITPFELTVEDRDTPDKLLKFTVTQVPVHGQLLFNNTRPAMIFTKQDLNENLISYRHDGTESSEDSFSFTVTDGSHTEFYVFPDTAFETRRPQVMKIRILAVDNRVPQIVVNKGASTLRTLATGHLGFMITSKVLKAEDRDSLHVSLRFIVTEAPRHGYLLNLGQGNHSVTEFTQADIDDMKICYVLREGANAVSDIFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAKKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGAHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSIEEDIGELFIPIRRSGDVSQELMVICYTRQGTARGTVPTSVLSYSDYISRPEDHTSVIRFDKDEREKMCRVIIIDDSLFEEEETFHVLLSMPMGGRIGSEFPEAQVTIRPDKDDEPVFYFGDVEYSVDESAGSVEVRVWRSGTDLSQASSVTVTSQKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPMLEGVEQFELVLRMPMNAALGDPSKATVIINDSVSDLPKMQFKERVYTGNENDGQIVAMIHRSGDIQYRSSVRCYTRQGSAQVMMDFEERPNTDISIITFLPGEIEKPCILGLMDDVLYEEIEELRLVLGTPQSNSPFGAAVGEQNETLIRIRDDADKTIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVEIEVVFDGIREMREAFTVHLKPDENMIAETQATKAIVYIEETNSVADATFPSVPQVVSLLMYDDPSRAKESAGPVSGYPVVCITACNPKYSDYEKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNADGSEGLELMSPIVTIGRGEGLCQPRVPGIVGAEPFSAKLRYTGPEDSEYTNLIKLTVTMPHIDGMLPVISTKELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHHGFLTDATKNPEIIGETYPYQYSSSIRGSNTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWTDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSVIMSVDHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTTPSHQEYRLPVTCNPREPITFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYNPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNANRGIGKRSVEYRHHSLVSQGKPQAATKSRKKREIRSSPPLAWEIGAENNRGTNIQHIALDRSSRKQILHGRVSPDGVLPRELNSPSSEVSLLTVVGGVAVGLLAVILTAVAVMMHQGKKGARRKDAPKDPGSREPMMPSVSHHSDSSEV

Target ORF information:

RefSeq Version	XM_012130745.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130745.2

ORF Insert Sequence:

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCCCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCCCGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT TGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG CGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCAAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCCTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGTGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGCGG TAACACTATC CAAGGAGTTA CTGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAC 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACC CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCGCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTCCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTAACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACACT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AACGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CCAGTCTGAA TATTCCATTG AAGAGGACAT TGGCGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAAGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TCTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCA TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CGATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTA TGGCGGAGCG GCACTGACCT GTCCCAGGCT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACGT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAGTATT CGATGGCATA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCTG GACCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTGTCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACGTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( XM_004012101.3 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004012150.1 Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.

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CloneID	OSm09286
Clone ID Related Accession (Same CDS sequence)	XM_004012101.3
Accession Version	XM_004012101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639019.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004012101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCCCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCAGGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGCGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT TGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG CGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA 
GACATGCTGG CAGCTGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCGAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC GGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCCTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCATG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGCGG TAACACTATC CAAGGAGTTA CTGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAC 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACC CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCGCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTCCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTAACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCTATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACACT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AATGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCT GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CCAGTCTGAA TATTCCATTG AAGAGGACAT TGGCGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAAGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TTTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGTTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAAAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CAATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGCGGAGCG GCACTGACCT GTCCCAGGCT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACGT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAATATT CGATGGCGTA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCCG GGCCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTATCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004012150.1
CDS	345..9863
Translation	MRATGTPGVTERTRPAGNPTKSPPTPPTPPGLVLLLLLSLVSRVRAQPPDPGRAVLSPDLAGVAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRQGRLSPKRFPCDFGAGAVRYSHLGARSPSRDRVRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLGALPRYGELLHYPQVPGPASKGDTQEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVAELRSRGSPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESAPDLLIFNLTSPFQPGQGFLVSTDDRSLPLSSFTQRDLHLLKIAYQPPSEDSDQERLFELELEVVDPEGAASDPFSFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVQLEVVAGLRHGQLVLLGASSGNSAPKTFTVAELAAGRVVYHHDDKDGSLSDNLILRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPQALSATDMDSEDSLLLFVLEAPSSTAGHLLLRQTHPPQGEEMLLGSPWRKQGVFYEKTVTEWRQRDITEGRLFYRHSGPHSPGPVIDQFTFHVQDNHDPPNKSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPMDTDENHSPAPLGTLVLTDDPSVVVAHFTQAQVNHHKIAYRPPDQELGVAARVAQFQFQVEDQAGNVAPGTFTFYLQPVDNQPPEILNTGFTVQEKSHHILSETELGVNDVDTDVAHITFTLIQVPKHGHMKLSGQVLHVGGIFHLEDIKQGRISYAHNEAESLTDSCSLEVSDGHHVVPITLRVNVRPMDGELPVLSLPAGTLGSYLDVLENGATEITANVIRGTNGDADDLMLTFLLEDAPLYGEILVNGVPAEQFTQRDILEGSVVYSHTSGEIGLLPKEDSFNLSLSDMSQEWRIGGNTIQGVTVWVTILPVDSQAPEISVGEQFIVLEGDKSVITSMHISAEDIDSLNDDILCTIIIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIVIIPTNDEQPEIFIREFMVMEGMSLVVDTPILNAADADVPADDLTFTITKVPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGRHSVEKTVLIMVIPVDDETPRMAINNGLEIEIGETKIINNRILMATDLDSEDKFLVYIIRYGPGHGLLQRQTPTGVFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSIGSPDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLESTDQPRVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGEKKLITPFELTVEDRDTPDKLLKFTVTQVPVHGQLLFNNTRPAMIFTKQDLNENLISYRHDGTESSEDSFSFTVTDGSHTEFYVFPDTAFETRRPQVMKIRILAVDNRVPQIVVNKGASTLRTLATGHLGFMITSKVLKAEDRDSLHVSLRFIVTEAPRHGYLLNLGQGNHSVTEFTQADIDDMKICYVLREGANAVSDIFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAKKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGAHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSIEEDIGELFIPIRRSGDVSQELMVICYTRQGTARGTVPTSVLSYSDYISRPEDHTSVIRFDKDEREKMCRVVIIDDSLFEKEETFHVLLSMPMGGRIGSEFPEAQVTIRPDKDDEPVFYFGDVEYSVDESAGSVEVRVWRSGTDLSQASSVTVTSQKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPMLEGVEQFELVLRMPMNAALGDPSKATVIINDSVSDLPKMQFKERVYTGNENDGQIVAMIHRSGDIQYRSSVRCYTRQGSAQVMMDFEERPNTDISIITFLPGEIEKPCILGLMDDVLYEEIEELRLVLGTPQSNSPFGAAVGEQNETLIRIRDDADKTIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVEIEVIFDGVREMREAFTVHLKPDENMIAETQATKAIVYIEETNSVADATFPSVPQVVSLLMYDDPSRAKESAGPVSGYPVVCITACNPKYSDYEKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNADGSEGLELMSPIVTIGRGEGLCQPRVPGIVGAEPFSAKLRYTGPEDSEYTNLIKLTVTMPHIDGMLPVISTKELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHHGFLTDATKNPEIIGETYPYQYSSSIRGSNTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWTDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSVIMSVDHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTTPSHQEYRLPVTCNPREPITFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYNPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNANRGIGKRSVEYRHHSLVSQGKPQAATKSRKKREIRSSPPLAWEIGAENNRGTNIQHIALDRSSRKQILHGRVSPDGVLPRELNSPSSEVSLLTVVGGVAVGLLAVILTAVAVMMHQGKKGARRKDAPKDPGSREPMMPSVSHHSDSSEV

Target ORF information:

RefSeq Version	XM_004012101.3
Organism	Ovis aries(sheep)
Definition	Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004012101.3

ORF Insert Sequence:

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCCCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCAGGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGCGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT TGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG CGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA 
GACATGCTGG CAGCTGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCGAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC GGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCCTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCATG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGCGG TAACACTATC CAAGGAGTTA CTGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAC 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACC CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCGCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTCCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTAACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCTATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACACT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AATGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCT GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CCAGTCTGAA TATTCCATTG AAGAGGACAT TGGCGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAAGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TTTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGTTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAAAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CAATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGCGGAGCG GCACTGACCT GTCCCAGGCT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACGT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAATATT CGATGGCGTA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCCG GGCCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTATCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( XM_027973602.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027829403.1 Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

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CloneID	OSm59409
Clone ID Related Accession (Same CDS sequence)	XM_027973602.1
Accession Version	XM_027973602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	FRAS1-related extracellular matrix protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCTCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCCCGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTCACCGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCTGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCGAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC GGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCTTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTG 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGTGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACAG ATGACAGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TGTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCTCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTTA CCGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAT 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCCCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTTCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACGGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACGCT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTGCATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AATGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCT GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CAAGTCTGAA TATTCCATTG AAGAGGACAT TGGTGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TTTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAAAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CAATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGCGGAGCG GCACTGACCT GTCCCAGACT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACAT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAATATT CGATGGCGTA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCCG GGCCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTGTCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TGGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACAGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027829403.1
CDS	1..9519
Translation	MRATGTPGVTERTRPAGNPTKSPPTPPTPPGLVLLLLLSLVSRVRAQPPDPGRAVLSPDLAGVAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGAGEVRYSHLGARSPSRDRVRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLGALPRYGELLHYPQVPGPASKGDTQEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGSPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESAPDLLIFNLTSPFQPGQGFLVSTDDRSLPLSSFTQRDLHLLKIAYQPPSEDSDQERLFELELEVVDPEGAASDPFSFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVQLEVVAGLRHGQLVLLGASSGNSAPKTFTVAELAAGRVVYHHDDKDGSLSDNLILRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPQALSATDMDSEDSLLLFVLEAPSSTAGHLLLRQTHPPQGEEMLLGSPWRKQGVFYEKTVTEWRQRDITEGRLFYRHSGPHSPGPVIDQFTFRVQDNHDPPNKSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPMDTDENHSPAPLGTLVLTDDPSVVVAHFTQAQVNHHKIAYRPPDQELGVAARVAQFQFQVEDQAGNVAPGTFTFYLQPVDNQPPEILNTGFTVQEKSHHILSETELGVNDVDTDVAHITFTVIQVPKHGHMKLSGQVLHVGGIFHLEDIKQGRISYAHNEAESLTDSCSLEVSDGHHVVPITLRVNVRLMDGELPVLSLPAGTLGSYLDVLENGATEITANVIRGTNGDADDLMLTFLLEDAPLYGEILVNGVPAEQFTQRDILEGSVVYSHTSGEIGLLPKEDSFNLSLSDMSQEWRIGGNTIQGVTVWVTILPVDSQAPEISVGEQFIVLEGDKSVITSMHISAEDIDSLNDDILCTIIIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIVIIPTNDEQPEIFIREFMVMEGMSLVVDTPILNAADADVPPDDLTFTITKVPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGRHSVEKTVLIMVIPVDDETPRMAINNGLEIEIGETKIINNRILMATDLDSEDKFLVYIIRYGPGHGLLQRQTPTGVFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSIGSPDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLESTDQPRVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGEKKLITPFELTVEDRDTPDKLLKFTVTQVPVHGQLLFNNTRPAMIFTKQDLNENLISYRHDGTESSEDSFSFTVTDGSHTEFYVFPDTAFETRRPQVMKIRILAVDNRVPQIVVNKGASTLRTLATGHLGFMITSKVLKAEDRDSLHVSLRFIVTEAPRHGYLLNLGQGNHSVTEFTQADIDDMKICYVLREGANAVSDIFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAKKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGAHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPKSEYSIEEDIGELFIPIRRSGDVSQELMVICYTRQGTARGTVPTSVLSYSDYISRPEDHTSVIRFDKDEREKMCRVVIIDDSLFEKEETFHVLLSMPMGGRIGSEFPEAQVTIRPDKDDEPVFYFGDVEYSVDESAGSVEVRVWRSGTDLSQTSSVTVTSQKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPMLEGVEQFELVLRMPMNAALGDPSKATVIINDSVSDLPKMQFKERVYTGNENDGQIVAMIHRSGDIQYRSSVRCYTRQGSAQVMMDFEERPNTDISIITFLPGEIEKPCILGLMDDILYEEIEELRLVLGTPQSNSPFGAAVGEQNETLIRIRDDADKTIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVEIEVIFDGVREMREAFTVHLKPDENMIAETQATKAIVYIEETNSVADATFPSVPQVVSLLMYDDPSRAKESAGPVSGYPVVCITACNPKYSDYEKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNADGSEGLELMSPIVTIGRGEGLCQPRVPGIVGAEPFSAKLRYTGPEDSEYTNLIKLTVTMPHIDGMLPVISTKELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHHGFLTDATKNPEIIGETYPYQYSSSIRGSNTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWTDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSVIMSVDHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTTPSHQEYRLPVTCNPREPITFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYNPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNANRGIGKRSVEYRHHSLVSQGKPQAATKSRKKREIRSSPPLAWEIGAENNRGTNIQHIALDRSSRKQILHGRVSPDSVLPRELNSPSSEVSLLTVVGGVAVGLLAVILTAVAVMMHQGKKGARRKDAPKDPGSREPMMPSVSHHSDSSEV

Target ORF information:

RefSeq Version	XM_027973602.1
Organism	Ovis aries(sheep)
Definition	Ovis aries FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027973602.1

ORF Insert Sequence:

ATGCGAGCCA CCGGGACCCC CGGGGTAACC GAGCGCACGC GCCCCGCAGG AAACCCCACC 
AAGTCTCCAC CGACGCCCCC GACGCCGCCC GGGCTGGTGC TGCTGCTTCT CCTGTCGCTG 
GTAAGCCGCG TCCGGGCACA GCCCCCTGAC CCCGGCCGGG CCGTGCTGTC CCCGGACCTC 
GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG 
GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA 
CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAACGACG CGCTGGCCCA GCGGCCCGGC 
CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTACTCGCAC 
CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG 
GGAGGGGCGG CCGTGCTCCC CTTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACGC GAAACCTGCC CCTGGTCGTG GAGGAGCTGC TGGGGACCAG CAACGCCCTG 
GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCG AGGAGTGTCG CGTGGGCATC 
CTGTCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTTC TTCACTACCC GCAGGTCCCG 
GGCCCGGCCA GCAAGGGGGA CACCCAGGAG CCCCTCCTGA TGGACTGCAA AGCATTCCAG 
GAGCTGGGCG TGCGCTACCG CCACACCGCC CCCAGTCGCT CTCCGAACAG GGACTGGGTG 
CCCATGGTGG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCAGC TTTGAAGCGC 
GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACTGC CCCCAAGCCC 
AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTCACCGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGAGT GCCCCAGACT TGCTGATCTT CAATCTCACC 
TCTCCGTTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG CCTGCCGCTC 
TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAGATTG CCTATCAGCC CCCCTCTGAA 
GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC GGAAGGTGCT 
GCCTCCGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG 
GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT 
GCAGGCAGTG GACCACAAAA CTTGGTCATC AGTGATGAAG ACGACTTAGA GGCTGTACAG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CAGCTTGTCC TTCTGGGTGC CTCCAGTGGC 
AACTCTGCCC CTAAGACCTT TACTGTAGCT GAGCTAGCAG CCGGCCGAGT GGTCTACCAT 
CACGATGACA AAGACGGCTC ACTAAGTGAC AACCTCATAC TCCGAATGGT GGACGGAGGA 
CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCAGTCCTCA ATGCCAACAC GGGGCTGACG CTGGCAGAGG GTGAAACTGT GCCCATCTTG 
CCCCAAGCTC TGAGTGCAAC TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTG 
GAGGCACCCT CCTCCACTGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAGGGG 
GAGGAGATGC TTCTCGGGAG CCCTTGGAGA AAACAGGGAG TGTTCTATGA GAAAACAGTG 
ACAGAGTGGC GGCAGCGAGA CATAACCGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC 
CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC 
CCTAATAAGT CTGGGCTACA GAGGTTCGTG ATACGCATTC ATCCCGTGGA CCGCCTCCCT 
CCTGAGCTGG GCAGTGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACAG ATGACAGAGA ACTCCGTTAC 
ACGGTGACCC AGCCCCCGAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC 
TTGGTCCTGA CTGACGATCC TTCGGTGGTG GTGGCCCATT TTACACAAGC TCAGGTCAAC 
CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC 
CAGTTTCAGT TCCAGGTGGA GGACCAAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT 
TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG 
GAGAAGAGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT 
GTGGCCCACA TCACCTTCAC TGTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT 
GGACAGGTAC TGCATGTTGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT 
TCCTACGCCC ATAATGAGGC TGAGTCTCTG ACCGATAGCT GCTCCTTGGA GGTCAGTGAT 
GGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCTCATGGA CGGTGAGCTG 
CCTGTGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG 
TTGACTTTCC TCTTGGAAGA TGCACCCCTA TATGGAGAAA TCTTGGTCAA TGGAGTTCCA 
GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TTGTCTACTC TCACACAAGT 
GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT 
CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTTA CCGTGTGGGT GACCATCCTC 
CCTGTTGACA GCCAGGCCCC AGAAATTTCT GTAGGTGAAC AGTTTATCGT TCTGGAAGGC 
GATAAAAGCG TTATAACCTC AATGCACATA AGTGCTGAAG ACATCGATTC TCTCAATGAT 
GACATCTTGT GCACCATCAT CATTCAGCCT ACCTCGGGTT ACGTGGAGAA CATCTCTCCA 
GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTATAA GTGCTTTCAC TCTGAAAGAT 
CTCAGGCAAG GTCACATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCAGTGGAA 
GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC 
ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT 
CCCCCAGATG ACTTAACTTT CACTATTACC AAGGTTCCCA CCCACGGTCA CATCATGAAC 
CAGCTGATAA ACGGCACGGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT 
TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTAAA 
CTCACAGATG GTAGGCACTC TGTGGAAAAG ACGGTTCTCA TTATGGTTAT TCCTGTTGAT 
GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATCGG GGAAACCAAA 
ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT 
TACATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACAAACACC GACGGGTGTC 
TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAATTTGATT 
CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT 
GGAATCAATG CACTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC 
TTCCCCGATG TGATAAGTAA AGGAGTGTCC TTGAAGGAAG GTGGTAAGGT CACCCTTACG 
ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC 
ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AACCTCGAGT GTCTATCACA 
TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAT 
GAGGTAAAGA TGGACAGTTT TGAGTTTCAA GTCACTGATG GACGTAACCC TGTCTTCCGG 
ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC 
CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC 
AGGGATACCC CAGACAAACT TTTGAAATTC ACTGTCACCC AGGTGCCTGT TCATGGTCAA 
CTCCTGTTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC 
TTAATCAGCT ACAGACATGA TGGTACTGAG TCAAGTGAAG ACAGCTTCTC CTTCACGGTG 
ACTGATGGTT CCCATACAGA GTTCTATGTT TTTCCTGATA CAGCATTTGA AACAAGAAGA 
CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTAGTG 
AACAAAGGGG CCTCTACGCT TCGCACTCTT GCCACAGGCC ACTTGGGGTT CATGATCACA 
AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTGCATG TTTCTCTCCG GTTTATTGTG 
ACGGAGGCCC CCCGACATGG ATATCTCCTC AACCTGGGCC AAGGCAACCA CAGTGTGACT 
GAGTTTACGC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCGTAA GTGACATATT CCACTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC 
AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC 
AATGAGGACT CCAAATTTCT AGATGTCGTG CTTAAGCGCA GAGGTTATTT GGGAGAGACT 
TCCTTTATAA GTATTGGCAC GAGAGATGGG ACTGCAAAGA AAGACAAGGA CTTCAAGGGC 
AAAGCACAGA AGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GAGAGTGCGG 
ATCCTGAGCG ACGGGGCACA CGAGCAGTCT GAGACCTTCC AGGTGGTTCT CTCAGAGCCC 
GTGCTGGCTG CCCTGGAGTT CCCCGCAGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT 
GAATCAACTG TGTTCATCCC CAAGTCTGAA TATTCCATTG AAGAGGACAT TGGTGAACTG 
TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGAT CTGTTATACA 
CGGCAAGGAA CAGCAAGAGG AACCGTGCCA ACATCTGTGC TTTCCTATTC TGATTACATA 
TCCCGGCCCG AGGACCATAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG 
TGTAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAAAGG AGGAAACATT CCACGTCCTC 
CTGAGCATGC CAATGGGTGG GAGAATTGGA TCAGAGTTCC CAGAGGCTCA GGTTACAATC 
AGACCCGACA AAGACGACGA GCCCGTCTTC TACTTTGGAG ACGTCGAGTA CTCTGTGGAT 
GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGCGGAGCG GCACTGACCT GTCCCAGACT 
TCCAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC 
TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCGTTCGT 
GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTGTTGAGCA GTTTGAATTG 
GTTCTCCGAA TGCCTATGAA TGCAGCTCTT GGGGACCCCA GCAAAGCCAC GGTGATCATA 
AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA TACTGGCAAT 
GAAAATGATG GGCAAATAGT CGCCATGATC CACCGGAGTG GGGATATCCA ATACAGGTCT 
TCAGTGAGAT GCTACACGCG GCAGGGCTCC GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC 
CTGGGGCTGA TGGATGACAT ATTGTACGAG GAGATAGAGG AGCTCCGACT GGTTCTTGGC 
ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA 
AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGTGTC 
AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AAGTGTCCAT TGTGAGAGTT CACACCAAGG ATGGCTCGGC CACTTCTGGA 
GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT 
GTGGAAATTG AAGTAATATT CGATGGCGTA AGAGAGATGA GAGAGGCCTT CACTGTCCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT TGTGTATATA 
GAAGAAACAA ACAGCGTGGC CGATGCCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG 
CTGATGTACG ATGACCCTTC CAGGGCCAAA GAGAGTGCCG GGCCTGTGTC TGGTTATCCT 
GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATTCAGACT ATGAAAAAAC AGGCTCTATT 
TGTGCAAGTG AGAACATCAA TGATACTTTG ACCCGGTACC GATGGCTGAT CAGCGCCCCC 
GCCGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT 
TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGCTCCAG AGTGCAGTGT 
GCAGCTCGGG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG 
ACCATCGGCA GAGGAGAAGG TCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG 
CCCTTCTCAG CTAAACTGCG CTACACGGGC CCTGAGGACT CAGAGTACAC GAACCTCATC 
AAGCTCACCG TCACGATGCC CCACATAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG 
CTTTCCAACT TTGAACTCAC TCTCAGCCCT GATGGCACCC GTGTTGGAAA CCACAAGTGC 
TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACT 
AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGT 
TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT 
AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTACGTG ACTCTTCGGG TCCCTCTGTA TGTCTCCTAC 
GTGTTCCACT CCCCCGTTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCTGAACTC 
CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA 
GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGACGT 
TTGGTCGTGA ACTTCAAGAC GGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT 
GCATCCTTCA CAAGCTCAGT GATCATGTCA GTCGATCATC CAGGTCTAAC CTTTTCCCTG 
CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTTGTGTCT 
GACTTTGCAG TCCGTGACTA CTCAGGTACC TACACAGTAA AACTGCTGCC GTGCACCACT 
CCATCTCATC AGGAATACCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT 
GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC 
ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGATGGATC CATGGGATTT 
GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTACGGTCG GGTCATGGTG 
GATCCGGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTGTGC 
ACTGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ATGCAGAATA CGGCTGCTTG 
GCTGATTCGC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAAACA 
CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCGGTGGA TGACCCTGAA 
GCTATCCTCT TGGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTTG CTCTGGGTCG AGAATGGTAC ATACACACCA TCTACACAGT AAGATCGAAA 
GACAACGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGCACCA CTCTTTGGTG 
AGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC 
CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAACCGGG GAACAAACAT CCAGCACATC 
GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACAGCGTC 
CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACAGT GGTGGGGGGC 
GTCGCGGTGG GACTCCTGGC GGTCATCCTC ACTGCCGTCG CGGTGATGAT GCACCAGGGC 
AAGAAGGGTG CCAGGAGGAA GGACGCGCCC AAGGACCCTG GCAGCCGTGA GCCCATGATG 
CCCTCGGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM2 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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