PORCN cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	PORCN
Entrez Gene ID	100018836
Full Name	porcupine O-acyltransferase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007507680.2	XP_007507742.1	protein-serine O-palmitoleoyltransferase porcupine isoform X1
XM_007507681.2	XP_007507743.1	protein-serine O-palmitoleoyltransferase porcupine isoform X2
XM_007507682.2	XP_007507744.1	protein-serine O-palmitoleoyltransferase porcupine isoform X4
XM_001371865.3	XP_001371902.1	protein-serine O-palmitoleoyltransferase porcupine isoform X3

Pathways from BioSystems
Gene Ontology

The following PORCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PORCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc35342	XM_001371865.3 Latest version!	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMc35343	XM_007507682.2 Latest version!	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMc35340	XM_007507680.2 Latest version!	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMc35341	XM_007507681.2 Latest version!	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PORCN ( XM_001371865.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001371902.1 Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMc35342
Clone ID Related Accession (Same CDS sequence)	XM_001371865.3
Accession Version	XM_001371865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	protein-serine O-palmitoleoyltransferase porcupine isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001371865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGCAAG AAACGGCAAG TGAGGGGTGC TGTGGCCAGG 
TGGCTGCATG CCTACGAGAG TGCCATCTCC TTCCACTTCA GTAATTACTT TGTGGGTTTC 
CTGTCAGAAG GCACAACCAC ACTGGCTGGG GCAGGCTTCA CGGAGGAGAA GGACCACTTG 
TGCTGGGACC TTACTGTGTC GAGGCCCCTG AGTGTGGAGC TTCCCAGGTC CATGGTTGAG 
GTTGTAACCA GCTGGAACCT GCCCATGTCA CACTGGCTCA ATTCCTATGT TTTCAAGAAT 
TCCCTTCGCC TTGGCAAGCT CTCAGCCCTC CTTGTGACCT ATGCTGCCAG CGCCCTCCTG 
CATGGCTTCA GTTTCCACTT GGGGGCCGTA CTGCTCACCC TCGCCTTCAT CACATATGTG 
GAACATGTGC TCCGGAAGAG GCTGGCTACC ATCTTCAATG CTTGTGTCCT CTCAAAGCGC 
TGCCCTCCGG ACTGCCCACA TCGACACCGC ACGGGTCTGG GCGTGCAGGC ACTGAACCTA 
TTCTTCAGGG CCCTAGCTGT CTTTCACTTG GCCTATCTGG GCTCCCTCTT TGACATAGAT 
ACTGACGACA CTACCGAGGA GCAGGGCTAC GGCATGTCCC ACACCGTCCG CAAGTGGTCC 
GAGCTCGGCT GGGCCAGTCA CTGGCTCACC TTCAGCTGCT GGATCTTCTA CCGCCTCATC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001371902.1
CDS	403..1788
Translation	MATFSRQDFFQQLVQGCVVPTAQQGLDQLWLLLAVCLTCRLLWRLGLPPAVKHASTVAGGFFSLYHFFQRHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGELSAVPSPVEFMGYLYFVGTIVFGPWLHFRSYLLAVEGRPLSFTWLGKVAASLSRALLCLVVSTCVGPYLFPYFIPLYGDRLLRSKKRQVRGAVARWLHAYESAISFHFSNYFVGFLSEGTTTLAGAGFTEEKDHLCWDLTVSRPLSVELPRSMVEVVTSWNLPMSHWLNSYVFKNSLRLGKLSALLVTYAASALLHGFSFHLGAVLLTLAFITYVEHVLRKRLATIFNACVLSKRCPPDCPHRHRTGLGVQALNLFFRALAVFHLAYLGSLFDIDTDDTTEEQGYGMSHTVRKWSELGWASHWLTFSCWIFYRLIS

Target ORF information:

RefSeq Version	XM_001371865.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001371865.3

ORF Insert Sequence:

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGCAAG AAACGGCAAG TGAGGGGTGC TGTGGCCAGG 
TGGCTGCATG CCTACGAGAG TGCCATCTCC TTCCACTTCA GTAATTACTT TGTGGGTTTC 
CTGTCAGAAG GCACAACCAC ACTGGCTGGG GCAGGCTTCA CGGAGGAGAA GGACCACTTG 
TGCTGGGACC TTACTGTGTC GAGGCCCCTG AGTGTGGAGC TTCCCAGGTC CATGGTTGAG 
GTTGTAACCA GCTGGAACCT GCCCATGTCA CACTGGCTCA ATTCCTATGT TTTCAAGAAT 
TCCCTTCGCC TTGGCAAGCT CTCAGCCCTC CTTGTGACCT ATGCTGCCAG CGCCCTCCTG 
CATGGCTTCA GTTTCCACTT GGGGGCCGTA CTGCTCACCC TCGCCTTCAT CACATATGTG 
GAACATGTGC TCCGGAAGAG GCTGGCTACC ATCTTCAATG CTTGTGTCCT CTCAAAGCGC 
TGCCCTCCGG ACTGCCCACA TCGACACCGC ACGGGTCTGG GCGTGCAGGC ACTGAACCTA 
TTCTTCAGGG CCCTAGCTGT CTTTCACTTG GCCTATCTGG GCTCCCTCTT TGACATAGAT 
ACTGACGACA CTACCGAGGA GCAGGGCTAC GGCATGTCCC ACACCGTCCG CAAGTGGTCC 
GAGCTCGGCT GGGCCAGTCA CTGGCTCACC TTCAGCTGCT GGATCTTCTA CCGCCTCATC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PORCN ( XM_007507682.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007507744.1 Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMc35343
Clone ID Related Accession (Same CDS sequence)	XM_007507682.2
Accession Version	XM_007507682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	protein-serine O-palmitoleoyltransferase porcupine isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007507682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGGTGG CTGCATGCCT ACGAGAGTGC CATCTCCTTC 
CACTTCAGTA ATTACTTTGT GGGTTTCCTG TCAGAAGGCA CAACCACACT GGCTGGGGCA 
GGCTTCACGG AGGAGAAGGA CCACTTGTGC TGGGACCTTA CTGTGTCGAG GCCCCTGAGT 
GTGGAGCTTC CCAGGTCCAT GGTTGAGGTT GTAACCAGCT GGAACCTGCC CATGTCACAC 
TGGCTCAATT CCTATGTTTT CAAGAATTCC CTTCGCCTTG GCAAGCTCTC AGCCCTCCTT 
GTGACCTATG CTGCCAGCGC CCTCCTGCAT GGCTTCAGTT TCCACTTGGG GGCCGTACTG 
CTCACCCTCG CCTTCATCAC ATATGTGGAA CATGTGCTCC GGAAGAGGCT GGCTACCATC 
TTCAATGCTT GTGTCCTCTC AAAGCGCTGC CCTCCGGACT GCCCACATCG ACACCGCACG 
GGTCTGGGCG TGCAGGCACT GAACCTATTC TTCAGGGCCC TAGCTGTCTT TCACTTGGCC 
TATCTGGGCT CCCTCTTTGA CATAGATACT GACGACACTA CCGAGGAGCA GGGCTACGGC 
ATGTCCCACA CCGTCCGCAA GTGGTCCGAG CTCGGCTGGG CCAGTCACTG GCTCACCTTC 
AGCTGCTGGA TCTTCTACCG CCTCATCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007507744.1
CDS	402..1754
Translation	MATFSRQDFFQQLVQGCVVPTAQQGLDQLWLLLAVCLTCRLLWRLGLPPAVKHASTVAGGFFSLYHFFQRHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGELSAVPSPVEFMGYLYFVGTIVFGPWLHFRSYLLAVEGRPLSFTWLGKVAASLSRALLCLVVSTCVGPYLFPYFIPLYGDRLLRRWLHAYESAISFHFSNYFVGFLSEGTTTLAGAGFTEEKDHLCWDLTVSRPLSVELPRSMVEVVTSWNLPMSHWLNSYVFKNSLRLGKLSALLVTYAASALLHGFSFHLGAVLLTLAFITYVEHVLRKRLATIFNACVLSKRCPPDCPHRHRTGLGVQALNLFFRALAVFHLAYLGSLFDIDTDDTTEEQGYGMSHTVRKWSELGWASHWLTFSCWIFYRLIS

Target ORF information:

RefSeq Version	XM_007507682.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007507682.2

ORF Insert Sequence:

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGGTGG CTGCATGCCT ACGAGAGTGC CATCTCCTTC 
CACTTCAGTA ATTACTTTGT GGGTTTCCTG TCAGAAGGCA CAACCACACT GGCTGGGGCA 
GGCTTCACGG AGGAGAAGGA CCACTTGTGC TGGGACCTTA CTGTGTCGAG GCCCCTGAGT 
GTGGAGCTTC CCAGGTCCAT GGTTGAGGTT GTAACCAGCT GGAACCTGCC CATGTCACAC 
TGGCTCAATT CCTATGTTTT CAAGAATTCC CTTCGCCTTG GCAAGCTCTC AGCCCTCCTT 
GTGACCTATG CTGCCAGCGC CCTCCTGCAT GGCTTCAGTT TCCACTTGGG GGCCGTACTG 
CTCACCCTCG CCTTCATCAC ATATGTGGAA CATGTGCTCC GGAAGAGGCT GGCTACCATC 
TTCAATGCTT GTGTCCTCTC AAAGCGCTGC CCTCCGGACT GCCCACATCG ACACCGCACG 
GGTCTGGGCG TGCAGGCACT GAACCTATTC TTCAGGGCCC TAGCTGTCTT TCACTTGGCC 
TATCTGGGCT CCCTCTTTGA CATAGATACT GACGACACTA CCGAGGAGCA GGGCTACGGC 
ATGTCCCACA CCGTCCGCAA GTGGTCCGAG CTCGGCTGGG CCAGTCACTG GCTCACCTTC 
AGCTGCTGGA TCTTCTACCG CCTCATCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PORCN ( XM_007507680.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007507742.1 Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc35340
Clone ID Related Accession (Same CDS sequence)	XM_007507680.2
Accession Version	XM_007507680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	protein-serine O-palmitoleoyltransferase porcupine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007507680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGCAAG AAACGGCAAG TGAGGGGTGC TGTGGCCAGG 
TGGCTGCATG CCTACGAGAG TGCCATCTCC TTCCACTTCA GTAATTACTT TGTGGGTTTC 
CTGTCAGAAG GCACAACCAC ACTGGCTGGG GCAGGCTTCA CGGAGGAGAA GGACCACTTG 
TGCTGGGACC TTACTGTGTC GAGGCCCCTG AGTGTGGAGC TTCCCAGGTC CATGGTTGAG 
GTTGTAACCA GCTGGAACCT GCCCATGTCA CACTGGCTCA ATTCCTATGT TTTCAAGAAT 
TCCCTTCGCC TTGGCAAGCT CTCAGCCCTC CTTGTGACCT ATGCTGCCAG CGCCCTCCTG 
CATGGCTTCA GTTTCCACTT GGGGGCCGTA CTGCTCACCC TCGCCTTCAT CACATATGTG 
GAACATGTGC TCCGGAAGAG GCTGGCTACC ATCTTCAATG CTTGTGTCCT CTCAAAGCGC 
TGCCCTCCGG ACTGCCCACA TCGACACCGC ACGGGTCTGG GCGTGCAGGC ACTGAACCTA 
TTCTTCAGGG CCCTAGCTGT CTTTCACTTG GCCTATCTGG GCTCCCTCTT TGACATAGAT 
ACTGACGACA CTACCGAGGA GCAGCCCCAG ACCCTGTCCT TCTCTTGGAA CCTTGGGCAA 
ATGGCAAGGG CCCTGCAGTC GCACTGGCCA CCACATTCCC CCAAGGCAGC CCTGCTGGCC 
CAGGCCCTAG AGCGGCTCCT GGAGGCAGAG GCTGGGTGGC AGGAATGGGA ACGGGAGACC 
AAGGCTGAAG CTGCTGCCCT GGCCCGGCTC CTCTTGCGTC TGTGGAGCAG CTCCCATCTG 
GAGCCTGAGG CCAACCTGGA GGAGGTGGTG GTACCTGGTC TCAAGGTCCA GGCCAAGGAT 
GAGGCAGCAG TTTCCAACCA GGAACTACTA CTCAGATTCC TGTTGGCCAA GATGGCAGAG 
GCCAGAAACA CTCCCACCTC CATGTGGGGG GAAGCCTGGC CACTTCAGCG GCACCGTCTG 
CGCCGGGACC TGGGGCCAGC TCCTGGGTCA GTGGCTCAGG GGTATACACT GCTCCGCCTG 
AACAAGGACA GGGCTCATGT CCAGCGAGGG GCACCGCTTG CTCCCACCCG CTACTGGCCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007507742.1
CDS	391..2196
Translation	MATFSRQDFFQQLVQGCVVPTAQQGLDQLWLLLAVCLTCRLLWRLGLPPAVKHASTVAGGFFSLYHFFQRHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGELSAVPSPVEFMGYLYFVGTIVFGPWLHFRSYLLAVEGRPLSFTWLGKVAASLSRALLCLVVSTCVGPYLFPYFIPLYGDRLLRSKKRQVRGAVARWLHAYESAISFHFSNYFVGFLSEGTTTLAGAGFTEEKDHLCWDLTVSRPLSVELPRSMVEVVTSWNLPMSHWLNSYVFKNSLRLGKLSALLVTYAASALLHGFSFHLGAVLLTLAFITYVEHVLRKRLATIFNACVLSKRCPPDCPHRHRTGLGVQALNLFFRALAVFHLAYLGSLFDIDTDDTTEEQPQTLSFSWNLGQMARALQSHWPPHSPKAALLAQALERLLEAEAGWQEWERETKAEAAALARLLLRLWSSSHLEPEANLEEVVVPGLKVQAKDEAAVSNQELLLRFLLAKMAEARNTPTSMWGEAWPLQRHRLRRDLGPAPGSVAQGYTLLRLNKDRAHVQRGAPLAPTRYWPE

Target ORF information:

RefSeq Version	XM_007507680.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007507680.2

ORF Insert Sequence:

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGCAAG AAACGGCAAG TGAGGGGTGC TGTGGCCAGG 
TGGCTGCATG CCTACGAGAG TGCCATCTCC TTCCACTTCA GTAATTACTT TGTGGGTTTC 
CTGTCAGAAG GCACAACCAC ACTGGCTGGG GCAGGCTTCA CGGAGGAGAA GGACCACTTG 
TGCTGGGACC TTACTGTGTC GAGGCCCCTG AGTGTGGAGC TTCCCAGGTC CATGGTTGAG 
GTTGTAACCA GCTGGAACCT GCCCATGTCA CACTGGCTCA ATTCCTATGT TTTCAAGAAT 
TCCCTTCGCC TTGGCAAGCT CTCAGCCCTC CTTGTGACCT ATGCTGCCAG CGCCCTCCTG 
CATGGCTTCA GTTTCCACTT GGGGGCCGTA CTGCTCACCC TCGCCTTCAT CACATATGTG 
GAACATGTGC TCCGGAAGAG GCTGGCTACC ATCTTCAATG CTTGTGTCCT CTCAAAGCGC 
TGCCCTCCGG ACTGCCCACA TCGACACCGC ACGGGTCTGG GCGTGCAGGC ACTGAACCTA 
TTCTTCAGGG CCCTAGCTGT CTTTCACTTG GCCTATCTGG GCTCCCTCTT TGACATAGAT 
ACTGACGACA CTACCGAGGA GCAGCCCCAG ACCCTGTCCT TCTCTTGGAA CCTTGGGCAA 
ATGGCAAGGG CCCTGCAGTC GCACTGGCCA CCACATTCCC CCAAGGCAGC CCTGCTGGCC 
CAGGCCCTAG AGCGGCTCCT GGAGGCAGAG GCTGGGTGGC AGGAATGGGA ACGGGAGACC 
AAGGCTGAAG CTGCTGCCCT GGCCCGGCTC CTCTTGCGTC TGTGGAGCAG CTCCCATCTG 
GAGCCTGAGG CCAACCTGGA GGAGGTGGTG GTACCTGGTC TCAAGGTCCA GGCCAAGGAT 
GAGGCAGCAG TTTCCAACCA GGAACTACTA CTCAGATTCC TGTTGGCCAA GATGGCAGAG 
GCCAGAAACA CTCCCACCTC CATGTGGGGG GAAGCCTGGC CACTTCAGCG GCACCGTCTG 
CGCCGGGACC TGGGGCCAGC TCCTGGGTCA GTGGCTCAGG GGTATACACT GCTCCGCCTG 
AACAAGGACA GGGCTCATGT CCAGCGAGGG GCACCGCTTG CTCCCACCCG CTACTGGCCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PORCN ( XM_007507681.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007507743.1 Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X2, mRNA.

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CloneID	OMc35341
Clone ID Related Accession (Same CDS sequence)	XM_007507681.2
Accession Version	XM_007507681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	protein-serine O-palmitoleoyltransferase porcupine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007507681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGGTGG CTGCATGCCT ACGAGAGTGC CATCTCCTTC 
CACTTCAGTA ATTACTTTGT GGGTTTCCTG TCAGAAGGCA CAACCACACT GGCTGGGGCA 
GGCTTCACGG AGGAGAAGGA CCACTTGTGC TGGGACCTTA CTGTGTCGAG GCCCCTGAGT 
GTGGAGCTTC CCAGGTCCAT GGTTGAGGTT GTAACCAGCT GGAACCTGCC CATGTCACAC 
TGGCTCAATT CCTATGTTTT CAAGAATTCC CTTCGCCTTG GCAAGCTCTC AGCCCTCCTT 
GTGACCTATG CTGCCAGCGC CCTCCTGCAT GGCTTCAGTT TCCACTTGGG GGCCGTACTG 
CTCACCCTCG CCTTCATCAC ATATGTGGAA CATGTGCTCC GGAAGAGGCT GGCTACCATC 
TTCAATGCTT GTGTCCTCTC AAAGCGCTGC CCTCCGGACT GCCCACATCG ACACCGCACG 
GGTCTGGGCG TGCAGGCACT GAACCTATTC TTCAGGGCCC TAGCTGTCTT TCACTTGGCC 
TATCTGGGCT CCCTCTTTGA CATAGATACT GACGACACTA CCGAGGAGCA GCCCCAGACC 
CTGTCCTTCT CTTGGAACCT TGGGCAAATG GCAAGGGCCC TGCAGTCGCA CTGGCCACCA 
CATTCCCCCA AGGCAGCCCT GCTGGCCCAG GCCCTAGAGC GGCTCCTGGA GGCAGAGGCT 
GGGTGGCAGG AATGGGAACG GGAGACCAAG GCTGAAGCTG CTGCCCTGGC CCGGCTCCTC 
TTGCGTCTGT GGAGCAGCTC CCATCTGGAG CCTGAGGCCA ACCTGGAGGA GGTGGTGGTA 
CCTGGTCTCA AGGTCCAGGC CAAGGATGAG GCAGCAGTTT CCAACCAGGA ACTACTACTC 
AGATTCCTGT TGGCCAAGAT GGCAGAGGCC AGAAACACTC CCACCTCCAT GTGGGGGGAA 
GCCTGGCCAC TTCAGCGGCA CCGTCTGCGC CGGGACCTGG GGCCAGCTCC TGGGTCAGTG 
GCTCAGGGGT ATACACTGCT CCGCCTGAAC AAGGACAGGG CTCATGTCCA GCGAGGGGCA 
CCGCTTGCTC CCACCCGCTA CTGGCCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007507743.1
CDS	391..2163
Translation	MATFSRQDFFQQLVQGCVVPTAQQGLDQLWLLLAVCLTCRLLWRLGLPPAVKHASTVAGGFFSLYHFFQRHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGELSAVPSPVEFMGYLYFVGTIVFGPWLHFRSYLLAVEGRPLSFTWLGKVAASLSRALLCLVVSTCVGPYLFPYFIPLYGDRLLRRWLHAYESAISFHFSNYFVGFLSEGTTTLAGAGFTEEKDHLCWDLTVSRPLSVELPRSMVEVVTSWNLPMSHWLNSYVFKNSLRLGKLSALLVTYAASALLHGFSFHLGAVLLTLAFITYVEHVLRKRLATIFNACVLSKRCPPDCPHRHRTGLGVQALNLFFRALAVFHLAYLGSLFDIDTDDTTEEQPQTLSFSWNLGQMARALQSHWPPHSPKAALLAQALERLLEAEAGWQEWERETKAEAAALARLLLRLWSSSHLEPEANLEEVVVPGLKVQAKDEAAVSNQELLLRFLLAKMAEARNTPTSMWGEAWPLQRHRLRRDLGPAPGSVAQGYTLLRLNKDRAHVQRGAPLAPTRYWPE

Target ORF information:

RefSeq Version	XM_007507681.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica porcupine homolog (Drosophila) (PORCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007507681.2

ORF Insert Sequence:

ATGGCTACCT TCAGCCGGCA GGACTTCTTC CAGCAGTTGG TGCAAGGCTG TGTGGTCCCC 
ACTGCCCAGC AGGGCCTGGA CCAGCTGTGG CTTCTGCTGG CTGTCTGCCT AACCTGCCGC 
CTCCTTTGGA GACTTGGGTT ACCCCCAGCT GTGAAACATG CCAGCACGGT GGCTGGTGGC 
TTCTTCAGCT TGTACCACTT CTTCCAACGA CACATGGTGT GGGTGGTGCT ACTGAGTCTC 
TTGTGCTATC TGGTCCTCTT CCTGTGCCGG CACTCATCCC ACCGAGGGGT CTTCCTCTCG 
GTCACCATCC TCATCTACCT GCTGATGGGG GAGATGCACA TGGTGGACAC TGTGACCTGG 
CACAAGATGA GGGGGGCCCA GATGATCGTC GCTATGAAAG CCGTGTCCCT GGGCTTTGAC 
CTGGATCGGG GTGAACTGAG TGCAGTGCCC TCCCCTGTGG AGTTCATGGG CTATCTCTAC 
TTCGTGGGCA CTATCGTCTT CGGGCCCTGG CTGCACTTCC GCAGTTACCT TCTGGCTGTG 
GAGGGCCGAC CACTGAGCTT CACATGGCTG GGGAAAGTGG CAGCAAGCCT GTCACGGGCC 
CTGCTCTGCT TGGTGGTCTC AACCTGTGTG GGCCCCTACC TCTTCCCATA CTTCATCCCA 
CTCTATGGGG ACAGGCTACT CCGAAGGTGG CTGCATGCCT ACGAGAGTGC CATCTCCTTC 
CACTTCAGTA ATTACTTTGT GGGTTTCCTG TCAGAAGGCA CAACCACACT GGCTGGGGCA 
GGCTTCACGG AGGAGAAGGA CCACTTGTGC TGGGACCTTA CTGTGTCGAG GCCCCTGAGT 
GTGGAGCTTC CCAGGTCCAT GGTTGAGGTT GTAACCAGCT GGAACCTGCC CATGTCACAC 
TGGCTCAATT CCTATGTTTT CAAGAATTCC CTTCGCCTTG GCAAGCTCTC AGCCCTCCTT 
GTGACCTATG CTGCCAGCGC CCTCCTGCAT GGCTTCAGTT TCCACTTGGG GGCCGTACTG 
CTCACCCTCG CCTTCATCAC ATATGTGGAA CATGTGCTCC GGAAGAGGCT GGCTACCATC 
TTCAATGCTT GTGTCCTCTC AAAGCGCTGC CCTCCGGACT GCCCACATCG ACACCGCACG 
GGTCTGGGCG TGCAGGCACT GAACCTATTC TTCAGGGCCC TAGCTGTCTT TCACTTGGCC 
TATCTGGGCT CCCTCTTTGA CATAGATACT GACGACACTA CCGAGGAGCA GCCCCAGACC 
CTGTCCTTCT CTTGGAACCT TGGGCAAATG GCAAGGGCCC TGCAGTCGCA CTGGCCACCA 
CATTCCCCCA AGGCAGCCCT GCTGGCCCAG GCCCTAGAGC GGCTCCTGGA GGCAGAGGCT 
GGGTGGCAGG AATGGGAACG GGAGACCAAG GCTGAAGCTG CTGCCCTGGC CCGGCTCCTC 
TTGCGTCTGT GGAGCAGCTC CCATCTGGAG CCTGAGGCCA ACCTGGAGGA GGTGGTGGTA 
CCTGGTCTCA AGGTCCAGGC CAAGGATGAG GCAGCAGTTT CCAACCAGGA ACTACTACTC 
AGATTCCTGT TGGCCAAGAT GGCAGAGGCC AGAAACACTC CCACCTCCAT GTGGGGGGAA 
GCCTGGCCAC TTCAGCGGCA CCGTCTGCGC CGGGACCTGG GGCCAGCTCC TGGGTCAGTG 
GCTCAGGGGT ATACACTGCT CCGCCTGAAC AAGGACAGGG CTCATGTCCA GCGAGGGGCA 
CCGCTTGCTC CCACCCGCTA CTGGCCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PORCN cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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