TSC2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC2
Entrez Gene ID	100011431
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001363281.3	XP_001363318.1	tuberin isoform X2
XM_007499380.2	XP_007499442.1	tuberin isoform X5
XM_007499377.2	XP_007499439.1	tuberin isoform X1
XM_007499381.2	XP_007499443.1	tuberin isoform X6
XM_007499378.2	XP_007499440.1	tuberin isoform X3
XM_007499379.2	XP_007499441.1	tuberin isoform X4

Pathways from BioSystems
Gene Ontology

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc28503	XM_007499377.2 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc28504	XM_001363281.3 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc28505	XM_007499378.2 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc28506	XM_007499379.2 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc28507	XM_007499380.2 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc28508	XM_007499381.2 Latest version!	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC2 ( XM_007499377.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499439.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OMc28503
Clone ID Related Accession (Same CDS sequence)	XM_007499377.2
Accession Version	XM_007499377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGC AGGATACAGA CATCCATCAC TAGTTCTAGT 
TTGGGTTCTG CAGATGAGAA TTCAATGGCT CAGGCTGATG ATAACCTAAA AAACCTTCAC 
CTGGAGCTCA CAGAAACTTG CTTGGATATG ATGGCCAGAT ACGTCTTTTC CAACTTCACT 
GCTGTACCCA AGAGATCTCC TGTGGGAGAA TTTCTTCTTG CTGGAGGGAG AACCAAAACC 
TGGTTGGTTG GGAACAAGTT AATAACTGTA ACAACAAGTG TTGGTACTGG GACCAGATCC 
CTGCTGGGCC TGGATTCTGG GGACCTGCAG AATAATGAGT CAAGCACAGA TCCCAGTTTG 
CAAGTGAGAC AGACAAAGGA AGCACCGGCC AAGTTAGAGT CGCAGGCTGG CCAGCAAGTT 
TGCCGGGGGT CTCGAGACCG AGTTCGGTCT ATGTCAGGGG GCCATGCTCT CCGGGTCAAT 
GCCTTGGATA CTCCAGCCTC CCACTTCCCA AGCAATCCCA CTTCTCCAGG ATTACAGAAT 
ACACCTGCTG CCCGTACAGA GAAGACTTCA GCAAGCGCCC AGACCCCTTT ACAGAAGGGA 
AAGACCAACC TGGCTGCCTA TGTGCCCCTG CTCACCCAGG GCTGGGCGGA GATCCTGGTC 
CGAAGGCCCA CAGGGAATAC CAGCTGGCTG ATGAGCTTGG AAAACCCTCT TAGTCCGTTC 
TCTTCAGATA TCAATAACAT GCCACTCCAG GAGCTCTCTA ATGCATTAAT GGCAGCTGAG 
CGTTTTAAGG AGCATCGGGA GACTGCCCTG TACAAGTCCT TGTCAGTGCC ATCATCTAGC 
CTTACCACGG GGACAGACAA GCCTTCCCTC CTACAGCGTT CCAATACAGT GGCCTCTTTC 
TCTTCCATGT ACCAGTCCAG TTGTCAAGGA AAGTTGCATA GGAGCGTTTC CTGGGCAGAG 
TCGGCAGTTG TTCTGGAAGA GGGAAGCCAA ACAGAGGTGG ACTTGAGAGA GACAGACTCG 
CCTTCCGAGT CTCAGGAAAT AGAGGATTTT GAAGCCATTG GCTCTCAGTC AACTGCAAGT 
GATGAGAGGT TTGGCAGACT TCAGGATGTC TATAGTAGGT CCTCTTCCAC TTCAAGTCAA 
GAAGAGAAAT CCTTCCGCTC TGAAGAGCTC ATTGCAGGTG GGATTCCCAT TGAGAGAGCT 
GTCTCTTCTG AAGATGGCAG GCCCATAGCT GAGCACTCCT TCCAGCCTTC TCAGCCCCTG 
AGCAAGTCCA GCTCTTCCCC TGAGTTACAG ACCCTACAGG ATGTCCTCAA GGATGCAGAG 
GATAAAGGCC ATCTTGGGAA GCTGAGCACA GATGTCAAAG CCAAGTCCCA GTCAGGGAAT 
TTAGAAGGAG AAGGAGCCAA CATCTGGCCA AGCCAAGGGG AGGAGAGCAG AGGACTTGGT 
GCAGGTCAGC AAGAGGCTGC AATGCCATCT TCAAGCTCGC CACGTTCCCC AAGTGGCCTC 
CGGCCTCGGG GCTACACTAT CTCTGACTCA GCCCCATCAA GGAGGGGCAA GAAGATGGAG 
AAGGATGCCT TCAAGAGTAG AGCTGCCACT TCTAACACAG AGAAGGTGCC CGGGATTAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAT TCCCCTTTCT TTGGAGATGA GTCCAACAAG 
CCACTCCTCT TACCTCCTGA GACATTCGAA AGGTCCGTGC AGCTCCTCGA TCAGATTCCC 
TCTTATGATA CCCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAACAGCGAG 
ATCGCCATCC TTTCCAATGA GCATGGCTCC TATAGATACA CAGAGTTCTT GACGGGCTTG 
GGGAAGCTCA TTGAACTCAA AGATTGCCAG CCAGACAAGA TCTACCTGGG GGGCCTGGAT 
GTGTGTGGGG AGGATGGGCA GTTCACCTAC TGCTGGCACG ATGATATCAT GCAAGCTATC 
TTTCACATTG CCACTCTAAT GCCCACCAAA GACTTGGACA AATACCGCTG TGACAAGAAG 
AGACACCTGG GGAATGACTT TGTATCAATT ATCTACAATG ATTCTGGAGA AGACTTCAAA 
TTGGGCACCA TCAAGGGCCA ATTCAATTTT GTCCACGTGA TAATCACTCC TCTGGATTAT 
GAATGTAACC TGGTCACACT GCAGTGTCGG AAAGATATGG AAGGCCTCGT GGATACCAGT 
GTGGCTAAAA TTGTATCAGA CCGAAACCTG CCCTTCGTTG CCCGCCAGAT GGCACTGCAT 
GCGAATATGG CATCGCAAGT TCATCACAGC AGATCAAATC CGACAGACAT CTACCCCTCC 
AAGTGGATTG CCAGATTGCG TCACATCAAA CGACTGCGGC ATAGGATCCG GGAAGAAACC 
CAGTATCCGA ACTCTGGCTT CCCCTTGATG CAGATGCATC CACCAGCTCA CCCCAAGTCC 
CCACCGCAGG CCCCATCCGA CCCCACCCCT ACCTATGAGA CTGGTCAGAG GAAACGGCTC 
ATCTCTTCAG TGGATGACTT TACAGAGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499439.1
CDS	240..5735
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSSLRIARPPKQGLNNSPPVKEFKESSAVDAFRSRSISVSEHVVRSRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTVASFSSMYQSSCQGKLHRSVSWAESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_007499377.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499377.2

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGC AGGATACAGA CATCCATCAC TAGTTCTAGT 
TTGGGTTCTG CAGATGAGAA TTCAATGGCT CAGGCTGATG ATAACCTAAA AAACCTTCAC 
CTGGAGCTCA CAGAAACTTG CTTGGATATG ATGGCCAGAT ACGTCTTTTC CAACTTCACT 
GCTGTACCCA AGAGATCTCC TGTGGGAGAA TTTCTTCTTG CTGGAGGGAG AACCAAAACC 
TGGTTGGTTG GGAACAAGTT AATAACTGTA ACAACAAGTG TTGGTACTGG GACCAGATCC 
CTGCTGGGCC TGGATTCTGG GGACCTGCAG AATAATGAGT CAAGCACAGA TCCCAGTTTG 
CAAGTGAGAC AGACAAAGGA AGCACCGGCC AAGTTAGAGT CGCAGGCTGG CCAGCAAGTT 
TGCCGGGGGT CTCGAGACCG AGTTCGGTCT ATGTCAGGGG GCCATGCTCT CCGGGTCAAT 
GCCTTGGATA CTCCAGCCTC CCACTTCCCA AGCAATCCCA CTTCTCCAGG ATTACAGAAT 
ACACCTGCTG CCCGTACAGA GAAGACTTCA GCAAGCGCCC AGACCCCTTT ACAGAAGGGA 
AAGACCAACC TGGCTGCCTA TGTGCCCCTG CTCACCCAGG GCTGGGCGGA GATCCTGGTC 
CGAAGGCCCA CAGGGAATAC CAGCTGGCTG ATGAGCTTGG AAAACCCTCT TAGTCCGTTC 
TCTTCAGATA TCAATAACAT GCCACTCCAG GAGCTCTCTA ATGCATTAAT GGCAGCTGAG 
CGTTTTAAGG AGCATCGGGA GACTGCCCTG TACAAGTCCT TGTCAGTGCC ATCATCTAGC 
CTTACCACGG GGACAGACAA GCCTTCCCTC CTACAGCGTT CCAATACAGT GGCCTCTTTC 
TCTTCCATGT ACCAGTCCAG TTGTCAAGGA AAGTTGCATA GGAGCGTTTC CTGGGCAGAG 
TCGGCAGTTG TTCTGGAAGA GGGAAGCCAA ACAGAGGTGG ACTTGAGAGA GACAGACTCG 
CCTTCCGAGT CTCAGGAAAT AGAGGATTTT GAAGCCATTG GCTCTCAGTC AACTGCAAGT 
GATGAGAGGT TTGGCAGACT TCAGGATGTC TATAGTAGGT CCTCTTCCAC TTCAAGTCAA 
GAAGAGAAAT CCTTCCGCTC TGAAGAGCTC ATTGCAGGTG GGATTCCCAT TGAGAGAGCT 
GTCTCTTCTG AAGATGGCAG GCCCATAGCT GAGCACTCCT TCCAGCCTTC TCAGCCCCTG 
AGCAAGTCCA GCTCTTCCCC TGAGTTACAG ACCCTACAGG ATGTCCTCAA GGATGCAGAG 
GATAAAGGCC ATCTTGGGAA GCTGAGCACA GATGTCAAAG CCAAGTCCCA GTCAGGGAAT 
TTAGAAGGAG AAGGAGCCAA CATCTGGCCA AGCCAAGGGG AGGAGAGCAG AGGACTTGGT 
GCAGGTCAGC AAGAGGCTGC AATGCCATCT TCAAGCTCGC CACGTTCCCC AAGTGGCCTC 
CGGCCTCGGG GCTACACTAT CTCTGACTCA GCCCCATCAA GGAGGGGCAA GAAGATGGAG 
AAGGATGCCT TCAAGAGTAG AGCTGCCACT TCTAACACAG AGAAGGTGCC CGGGATTAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAT TCCCCTTTCT TTGGAGATGA GTCCAACAAG 
CCACTCCTCT TACCTCCTGA GACATTCGAA AGGTCCGTGC AGCTCCTCGA TCAGATTCCC 
TCTTATGATA CCCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAACAGCGAG 
ATCGCCATCC TTTCCAATGA GCATGGCTCC TATAGATACA CAGAGTTCTT GACGGGCTTG 
GGGAAGCTCA TTGAACTCAA AGATTGCCAG CCAGACAAGA TCTACCTGGG GGGCCTGGAT 
GTGTGTGGGG AGGATGGGCA GTTCACCTAC TGCTGGCACG ATGATATCAT GCAAGCTATC 
TTTCACATTG CCACTCTAAT GCCCACCAAA GACTTGGACA AATACCGCTG TGACAAGAAG 
AGACACCTGG GGAATGACTT TGTATCAATT ATCTACAATG ATTCTGGAGA AGACTTCAAA 
TTGGGCACCA TCAAGGGCCA ATTCAATTTT GTCCACGTGA TAATCACTCC TCTGGATTAT 
GAATGTAACC TGGTCACACT GCAGTGTCGG AAAGATATGG AAGGCCTCGT GGATACCAGT 
GTGGCTAAAA TTGTATCAGA CCGAAACCTG CCCTTCGTTG CCCGCCAGAT GGCACTGCAT 
GCGAATATGG CATCGCAAGT TCATCACAGC AGATCAAATC CGACAGACAT CTACCCCTCC 
AAGTGGATTG CCAGATTGCG TCACATCAAA CGACTGCGGC ATAGGATCCG GGAAGAAACC 
CAGTATCCGA ACTCTGGCTT CCCCTTGATG CAGATGCATC CACCAGCTCA CCCCAAGTCC 
CCACCGCAGG CCCCATCCGA CCCCACCCCT ACCTATGAGA CTGGTCAGAG GAAACGGCTC 
ATCTCTTCAG TGGATGACTT TACAGAGTTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_001363281.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001363318.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OMc28504
Clone ID Related Accession (Same CDS sequence)	XM_001363281.3
Accession Version	XM_001363281.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001363281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTTT AAGGATAGCC AGACCCCCCA AGCAAGGCTT GAATAACTCT 
CCTCCCGTGA AGGAATTCAA AGAGAGCTCT GCAGTTGATG CCTTCCGGTC CCGCAGCATC 
AGTGTGTCTG AACATGTGGT TCGCAGCAGG ATACAGACAT CCATCACTAG TTCTAGTTTG 
GGTTCTGCAG ATGAGAATTC AATGGCTCAG GCTGATGATA ACCTAAAAAA CCTTCACCTG 
GAGCTCACAG AAACTTGCTT GGATATGATG GCCAGATACG TCTTTTCCAA CTTCACTGCT 
GTACCCAAGA GATCTCCTGT GGGAGAATTT CTTCTTGCTG GAGGGAGAAC CAAAACCTGG 
TTGGTTGGGA ACAAGTTAAT AACTGTAACA ACAAGTGTTG GTACTGGGAC CAGATCCCTG 
CTGGGCCTGG ATTCTGGGGA CCTGCAGAAT AATGAGTCAA GCACAGATCC CAGTTTGCAA 
GTGAGACAGA CAAAGGAAGC ACCGGCCAAG TTAGAGTCGC AGGCTGGCCA GCAAGTTTGC 
CGGGGGTCTC GAGACCGAGT TCGGTCTATG TCAGGGGGCC ATGCTCTCCG GGTCAATGCC 
TTGGATACTC CAGCCTCCCA CTTCCCAAGC AATCCCACTT CTCCAGGATT ACAGAATACA 
CCTGCTGCCC GTACAGAGAA GACTTCAGCA AGCGCCCAGA CCCCTTTACA GAAGGGAAAG 
ACCAACCTGG CTGCCTATGT GCCCCTGCTC ACCCAGGGCT GGGCGGAGAT CCTGGTCCGA 
AGGCCCACAG GGAATACCAG CTGGCTGATG AGCTTGGAAA ACCCTCTTAG TCCGTTCTCT 
TCAGATATCA ATAACATGCC ACTCCAGGAG CTCTCTAATG CATTAATGGC AGCTGAGCGT 
TTTAAGGAGC ATCGGGAGAC TGCCCTGTAC AAGTCCTTGT CAGTGCCATC ATCTAGCCTT 
ACCACGGGGA CAGACAAGCC TTCCCTCCTA CAGCGTTCCA ATACAGTGGC CTCTTTCTCT 
TCCATGTACC AGTCCAGTTG TCAAGGAAAG TTGCATAGGA GCGTTTCCTG GGCAGAGTCG 
GCAGTTGTTC TGGAAGAGGG AAGCCAAACA GAGGTGGACT TGAGAGAGAC AGACTCGCCT 
TCCGAGTCTC AGGAAATAGA GGATTTTGAA GCCATTGGCT CTCAGTCAAC TGCAAGTGAT 
GAGAGGTTTG GCAGACTTCA GGATGTCTAT AGTAGGTCCT CTTCCACTTC AAGTCAAGAA 
GAGAAATCCT TCCGCTCTGA AGAGCTCATT GCAGGTGGGA TTCCCATTGA GAGAGCTGTC 
TCTTCTGAAG ATGGCAGGCC CATAGCTGAG CACTCCTTCC AGCCTTCTCA GCCCCTGAGC 
AAGTCCAGCT CTTCCCCTGA GTTACAGACC CTACAGGATG TCCTCAAGGA TGCAGAGGAT 
AAAGGCCATC TTGGGAAGCT GAGCACAGAT GTCAAAGCCA AGTCCCAGTC AGGGAATTTA 
GAAGGAGAAG GAGCCAACAT CTGGCCAAGC CAAGGGGAGG AGAGCAGAGG ACTTGGTGCA 
GGTCAGCAAG AGGCTGCAAT GCCATCTTCA AGCTCGCCAC GTTCCCCAAG TGGCCTCCGG 
CCTCGGGGCT ACACTATCTC TGACTCAGCC CCATCAAGGA GGGGCAAGAA GATGGAGAAG 
GATGCCTTCA AGAGTAGAGC TGCCACTTCT AACACAGAGA AGGTGCCCGG GATTAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCATTCC CCTTTCTTTG GAGATGAGTC CAACAAGCCA 
CTCCTCTTAC CTCCTGAGAC ATTCGAAAGG TCCGTGCAGC TCCTCGATCA GATTCCCTCT 
TATGATACCC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAA CAGCGAGATC 
GCCATCCTTT CCAATGAGCA TGGCTCCTAT AGATACACAG AGTTCTTGAC GGGCTTGGGG 
AAGCTCATTG AACTCAAAGA TTGCCAGCCA GACAAGATCT ACCTGGGGGG CCTGGATGTG 
TGTGGGGAGG ATGGGCAGTT CACCTACTGC TGGCACGATG ATATCATGCA AGCTATCTTT 
CACATTGCCA CTCTAATGCC CACCAAAGAC TTGGACAAAT ACCGCTGTGA CAAGAAGAGA 
CACCTGGGGA ATGACTTTGT ATCAATTATC TACAATGATT CTGGAGAAGA CTTCAAATTG 
GGCACCATCA AGGGCCAATT CAATTTTGTC CACGTGATAA TCACTCCTCT GGATTATGAA 
TGTAACCTGG TCACACTGCA GTGTCGGAAA GATATGGAAG GCCTCGTGGA TACCAGTGTG 
GCTAAAATTG TATCAGACCG AAACCTGCCC TTCGTTGCCC GCCAGATGGC ACTGCATGCG 
AATATGGCAT CGCAAGTTCA TCACAGCAGA TCAAATCCGA CAGACATCTA CCCCTCCAAG 
TGGATTGCCA GATTGCGTCA CATCAAACGA CTGCGGCATA GGATCCGGGA AGAAACCCAG 
TATCCGAACT CTGGCTTCCC CTTGATGCAG ATGCATCCAC CAGCTCACCC CAAGTCCCCA 
CCGCAGGCCC CATCCGACCC CACCCCTACC TATGAGACTG GTCAGAGGAA ACGGCTCATC 
TCTTCAGTGG ATGACTTTAC AGAGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001363318.1
CDS	240..5732
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAVDAFRSRSISVSEHVVRSRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTVASFSSMYQSSCQGKLHRSVSWAESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_001363281.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001363281.3

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTTT AAGGATAGCC AGACCCCCCA AGCAAGGCTT GAATAACTCT 
CCTCCCGTGA AGGAATTCAA AGAGAGCTCT GCAGTTGATG CCTTCCGGTC CCGCAGCATC 
AGTGTGTCTG AACATGTGGT TCGCAGCAGG ATACAGACAT CCATCACTAG TTCTAGTTTG 
GGTTCTGCAG ATGAGAATTC AATGGCTCAG GCTGATGATA ACCTAAAAAA CCTTCACCTG 
GAGCTCACAG AAACTTGCTT GGATATGATG GCCAGATACG TCTTTTCCAA CTTCACTGCT 
GTACCCAAGA GATCTCCTGT GGGAGAATTT CTTCTTGCTG GAGGGAGAAC CAAAACCTGG 
TTGGTTGGGA ACAAGTTAAT AACTGTAACA ACAAGTGTTG GTACTGGGAC CAGATCCCTG 
CTGGGCCTGG ATTCTGGGGA CCTGCAGAAT AATGAGTCAA GCACAGATCC CAGTTTGCAA 
GTGAGACAGA CAAAGGAAGC ACCGGCCAAG TTAGAGTCGC AGGCTGGCCA GCAAGTTTGC 
CGGGGGTCTC GAGACCGAGT TCGGTCTATG TCAGGGGGCC ATGCTCTCCG GGTCAATGCC 
TTGGATACTC CAGCCTCCCA CTTCCCAAGC AATCCCACTT CTCCAGGATT ACAGAATACA 
CCTGCTGCCC GTACAGAGAA GACTTCAGCA AGCGCCCAGA CCCCTTTACA GAAGGGAAAG 
ACCAACCTGG CTGCCTATGT GCCCCTGCTC ACCCAGGGCT GGGCGGAGAT CCTGGTCCGA 
AGGCCCACAG GGAATACCAG CTGGCTGATG AGCTTGGAAA ACCCTCTTAG TCCGTTCTCT 
TCAGATATCA ATAACATGCC ACTCCAGGAG CTCTCTAATG CATTAATGGC AGCTGAGCGT 
TTTAAGGAGC ATCGGGAGAC TGCCCTGTAC AAGTCCTTGT CAGTGCCATC ATCTAGCCTT 
ACCACGGGGA CAGACAAGCC TTCCCTCCTA CAGCGTTCCA ATACAGTGGC CTCTTTCTCT 
TCCATGTACC AGTCCAGTTG TCAAGGAAAG TTGCATAGGA GCGTTTCCTG GGCAGAGTCG 
GCAGTTGTTC TGGAAGAGGG AAGCCAAACA GAGGTGGACT TGAGAGAGAC AGACTCGCCT 
TCCGAGTCTC AGGAAATAGA GGATTTTGAA GCCATTGGCT CTCAGTCAAC TGCAAGTGAT 
GAGAGGTTTG GCAGACTTCA GGATGTCTAT AGTAGGTCCT CTTCCACTTC AAGTCAAGAA 
GAGAAATCCT TCCGCTCTGA AGAGCTCATT GCAGGTGGGA TTCCCATTGA GAGAGCTGTC 
TCTTCTGAAG ATGGCAGGCC CATAGCTGAG CACTCCTTCC AGCCTTCTCA GCCCCTGAGC 
AAGTCCAGCT CTTCCCCTGA GTTACAGACC CTACAGGATG TCCTCAAGGA TGCAGAGGAT 
AAAGGCCATC TTGGGAAGCT GAGCACAGAT GTCAAAGCCA AGTCCCAGTC AGGGAATTTA 
GAAGGAGAAG GAGCCAACAT CTGGCCAAGC CAAGGGGAGG AGAGCAGAGG ACTTGGTGCA 
GGTCAGCAAG AGGCTGCAAT GCCATCTTCA AGCTCGCCAC GTTCCCCAAG TGGCCTCCGG 
CCTCGGGGCT ACACTATCTC TGACTCAGCC CCATCAAGGA GGGGCAAGAA GATGGAGAAG 
GATGCCTTCA AGAGTAGAGC TGCCACTTCT AACACAGAGA AGGTGCCCGG GATTAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCATTCC CCTTTCTTTG GAGATGAGTC CAACAAGCCA 
CTCCTCTTAC CTCCTGAGAC ATTCGAAAGG TCCGTGCAGC TCCTCGATCA GATTCCCTCT 
TATGATACCC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAA CAGCGAGATC 
GCCATCCTTT CCAATGAGCA TGGCTCCTAT AGATACACAG AGTTCTTGAC GGGCTTGGGG 
AAGCTCATTG AACTCAAAGA TTGCCAGCCA GACAAGATCT ACCTGGGGGG CCTGGATGTG 
TGTGGGGAGG ATGGGCAGTT CACCTACTGC TGGCACGATG ATATCATGCA AGCTATCTTT 
CACATTGCCA CTCTAATGCC CACCAAAGAC TTGGACAAAT ACCGCTGTGA CAAGAAGAGA 
CACCTGGGGA ATGACTTTGT ATCAATTATC TACAATGATT CTGGAGAAGA CTTCAAATTG 
GGCACCATCA AGGGCCAATT CAATTTTGTC CACGTGATAA TCACTCCTCT GGATTATGAA 
TGTAACCTGG TCACACTGCA GTGTCGGAAA GATATGGAAG GCCTCGTGGA TACCAGTGTG 
GCTAAAATTG TATCAGACCG AAACCTGCCC TTCGTTGCCC GCCAGATGGC ACTGCATGCG 
AATATGGCAT CGCAAGTTCA TCACAGCAGA TCAAATCCGA CAGACATCTA CCCCTCCAAG 
TGGATTGCCA GATTGCGTCA CATCAAACGA CTGCGGCATA GGATCCGGGA AGAAACCCAG 
TATCCGAACT CTGGCTTCCC CTTGATGCAG ATGCATCCAC CAGCTCACCC CAAGTCCCCA 
CCGCAGGCCC CATCCGACCC CACCCCTACC TATGAGACTG GTCAGAGGAA ACGGCTCATC 
TCTTCAGTGG ATGACTTTAC AGAGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_007499378.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499440.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OMc28505
Clone ID Related Accession (Same CDS sequence)	XM_007499378.2
Accession Version	XM_007499378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGG ATACAGACAT CCATCACTAG TTCTAGTTTG 
GGTTCTGCAG ATGAGAATTC AATGGCTCAG GCTGATGATA ACCTAAAAAA CCTTCACCTG 
GAGCTCACAG AAACTTGCTT GGATATGATG GCCAGATACG TCTTTTCCAA CTTCACTGCT 
GTACCCAAGA GATCTCCTGT GGGAGAATTT CTTCTTGCTG GAGGGAGAAC CAAAACCTGG 
TTGGTTGGGA ACAAGTTAAT AACTGTAACA ACAAGTGTTG GTACTGGGAC CAGATCCCTG 
CTGGGCCTGG ATTCTGGGGA CCTGCAGAAT AATGAGTCAA GCACAGATCC CAGTTTGCAA 
GTGAGACAGA CAAAGGAAGC ACCGGCCAAG TTAGAGTCGC AGGCTGGCCA GCAAGTTTGC 
CGGGGGTCTC GAGACCGAGT TCGGTCTATG TCAGGGGGCC ATGCTCTCCG GGTCAATGCC 
TTGGATACTC CAGCCTCCCA CTTCCCAAGC AATCCCACTT CTCCAGGATT ACAGAATACA 
CCTGCTGCCC GTACAGAGAA GACTTCAGCA AGCGCCCAGA CCCCTTTACA GAAGGGAAAG 
ACCAACCTGG CTGCCTATGT GCCCCTGCTC ACCCAGGGCT GGGCGGAGAT CCTGGTCCGA 
AGGCCCACAG GGAATACCAG CTGGCTGATG AGCTTGGAAA ACCCTCTTAG TCCGTTCTCT 
TCAGATATCA ATAACATGCC ACTCCAGGAG CTCTCTAATG CATTAATGGC AGCTGAGCGT 
TTTAAGGAGC ATCGGGAGAC TGCCCTGTAC AAGTCCTTGT CAGTGCCATC ATCTAGCCTT 
ACCACGGGGA CAGACAAGCC TTCCCTCCTA CAGCGTTCCA ATACAGTGGC CTCTTTCTCT 
TCCATGTACC AGTCCAGTTG TCAAGGAAAG TTGCATAGGA GCGTTTCCTG GGCAGAGTCG 
GCAGTTGTTC TGGAAGAGGG AAGCCAAACA GAGGTGGACT TGAGAGAGAC AGACTCGCCT 
TCCGAGTCTC AGGAAATAGA GGATTTTGAA GCCATTGGCT CTCAGTCAAC TGCAAGTGAT 
GAGAGGTTTG GCAGACTTCA GGATGTCTAT AGTAGGTCCT CTTCCACTTC AAGTCAAGAA 
GAGAAATCCT TCCGCTCTGA AGAGCTCATT GCAGGTGGGA TTCCCATTGA GAGAGCTGTC 
TCTTCTGAAG ATGGCAGGCC CATAGCTGAG CACTCCTTCC AGCCTTCTCA GCCCCTGAGC 
AAGTCCAGCT CTTCCCCTGA GTTACAGACC CTACAGGATG TCCTCAAGGA TGCAGAGGAT 
AAAGGCCATC TTGGGAAGCT GAGCACAGAT GTCAAAGCCA AGTCCCAGTC AGGGAATTTA 
GAAGGAGAAG GAGCCAACAT CTGGCCAAGC CAAGGGGAGG AGAGCAGAGG ACTTGGTGCA 
GGTCAGCAAG AGGCTGCAAT GCCATCTTCA AGCTCGCCAC GTTCCCCAAG TGGCCTCCGG 
CCTCGGGGCT ACACTATCTC TGACTCAGCC CCATCAAGGA GGGGCAAGAA GATGGAGAAG 
GATGCCTTCA AGAGTAGAGC TGCCACTTCT AACACAGAGA AGGTGCCCGG GATTAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCATTCC CCTTTCTTTG GAGATGAGTC CAACAAGCCA 
CTCCTCTTAC CTCCTGAGAC ATTCGAAAGG TCCGTGCAGC TCCTCGATCA GATTCCCTCT 
TATGATACCC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAA CAGCGAGATC 
GCCATCCTTT CCAATGAGCA TGGCTCCTAT AGATACACAG AGTTCTTGAC GGGCTTGGGG 
AAGCTCATTG AACTCAAAGA TTGCCAGCCA GACAAGATCT ACCTGGGGGG CCTGGATGTG 
TGTGGGGAGG ATGGGCAGTT CACCTACTGC TGGCACGATG ATATCATGCA AGCTATCTTT 
CACATTGCCA CTCTAATGCC CACCAAAGAC TTGGACAAAT ACCGCTGTGA CAAGAAGAGA 
CACCTGGGGA ATGACTTTGT ATCAATTATC TACAATGATT CTGGAGAAGA CTTCAAATTG 
GGCACCATCA AGGGCCAATT CAATTTTGTC CACGTGATAA TCACTCCTCT GGATTATGAA 
TGTAACCTGG TCACACTGCA GTGTCGGAAA GATATGGAAG GCCTCGTGGA TACCAGTGTG 
GCTAAAATTG TATCAGACCG AAACCTGCCC TTCGTTGCCC GCCAGATGGC ACTGCATGCG 
AATATGGCAT CGCAAGTTCA TCACAGCAGA TCAAATCCGA CAGACATCTA CCCCTCCAAG 
TGGATTGCCA GATTGCGTCA CATCAAACGA CTGCGGCATA GGATCCGGGA AGAAACCCAG 
TATCCGAACT CTGGCTTCCC CTTGATGCAG ATGCATCCAC CAGCTCACCC CAAGTCCCCA 
CCGCAGGCCC CATCCGACCC CACCCCTACC TATGAGACTG GTCAGAGGAA ACGGCTCATC 
TCTTCAGTGG ATGACTTTAC AGAGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499440.1
CDS	240..5732
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSSLRIARPPKQGLNNSPPVKEFKESSAVDAFRSRSISVSEHVVRRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTVASFSSMYQSSCQGKLHRSVSWAESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_007499378.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499378.2

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGG ATACAGACAT CCATCACTAG TTCTAGTTTG 
GGTTCTGCAG ATGAGAATTC AATGGCTCAG GCTGATGATA ACCTAAAAAA CCTTCACCTG 
GAGCTCACAG AAACTTGCTT GGATATGATG GCCAGATACG TCTTTTCCAA CTTCACTGCT 
GTACCCAAGA GATCTCCTGT GGGAGAATTT CTTCTTGCTG GAGGGAGAAC CAAAACCTGG 
TTGGTTGGGA ACAAGTTAAT AACTGTAACA ACAAGTGTTG GTACTGGGAC CAGATCCCTG 
CTGGGCCTGG ATTCTGGGGA CCTGCAGAAT AATGAGTCAA GCACAGATCC CAGTTTGCAA 
GTGAGACAGA CAAAGGAAGC ACCGGCCAAG TTAGAGTCGC AGGCTGGCCA GCAAGTTTGC 
CGGGGGTCTC GAGACCGAGT TCGGTCTATG TCAGGGGGCC ATGCTCTCCG GGTCAATGCC 
TTGGATACTC CAGCCTCCCA CTTCCCAAGC AATCCCACTT CTCCAGGATT ACAGAATACA 
CCTGCTGCCC GTACAGAGAA GACTTCAGCA AGCGCCCAGA CCCCTTTACA GAAGGGAAAG 
ACCAACCTGG CTGCCTATGT GCCCCTGCTC ACCCAGGGCT GGGCGGAGAT CCTGGTCCGA 
AGGCCCACAG GGAATACCAG CTGGCTGATG AGCTTGGAAA ACCCTCTTAG TCCGTTCTCT 
TCAGATATCA ATAACATGCC ACTCCAGGAG CTCTCTAATG CATTAATGGC AGCTGAGCGT 
TTTAAGGAGC ATCGGGAGAC TGCCCTGTAC AAGTCCTTGT CAGTGCCATC ATCTAGCCTT 
ACCACGGGGA CAGACAAGCC TTCCCTCCTA CAGCGTTCCA ATACAGTGGC CTCTTTCTCT 
TCCATGTACC AGTCCAGTTG TCAAGGAAAG TTGCATAGGA GCGTTTCCTG GGCAGAGTCG 
GCAGTTGTTC TGGAAGAGGG AAGCCAAACA GAGGTGGACT TGAGAGAGAC AGACTCGCCT 
TCCGAGTCTC AGGAAATAGA GGATTTTGAA GCCATTGGCT CTCAGTCAAC TGCAAGTGAT 
GAGAGGTTTG GCAGACTTCA GGATGTCTAT AGTAGGTCCT CTTCCACTTC AAGTCAAGAA 
GAGAAATCCT TCCGCTCTGA AGAGCTCATT GCAGGTGGGA TTCCCATTGA GAGAGCTGTC 
TCTTCTGAAG ATGGCAGGCC CATAGCTGAG CACTCCTTCC AGCCTTCTCA GCCCCTGAGC 
AAGTCCAGCT CTTCCCCTGA GTTACAGACC CTACAGGATG TCCTCAAGGA TGCAGAGGAT 
AAAGGCCATC TTGGGAAGCT GAGCACAGAT GTCAAAGCCA AGTCCCAGTC AGGGAATTTA 
GAAGGAGAAG GAGCCAACAT CTGGCCAAGC CAAGGGGAGG AGAGCAGAGG ACTTGGTGCA 
GGTCAGCAAG AGGCTGCAAT GCCATCTTCA AGCTCGCCAC GTTCCCCAAG TGGCCTCCGG 
CCTCGGGGCT ACACTATCTC TGACTCAGCC CCATCAAGGA GGGGCAAGAA GATGGAGAAG 
GATGCCTTCA AGAGTAGAGC TGCCACTTCT AACACAGAGA AGGTGCCCGG GATTAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCATTCC CCTTTCTTTG GAGATGAGTC CAACAAGCCA 
CTCCTCTTAC CTCCTGAGAC ATTCGAAAGG TCCGTGCAGC TCCTCGATCA GATTCCCTCT 
TATGATACCC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAA CAGCGAGATC 
GCCATCCTTT CCAATGAGCA TGGCTCCTAT AGATACACAG AGTTCTTGAC GGGCTTGGGG 
AAGCTCATTG AACTCAAAGA TTGCCAGCCA GACAAGATCT ACCTGGGGGG CCTGGATGTG 
TGTGGGGAGG ATGGGCAGTT CACCTACTGC TGGCACGATG ATATCATGCA AGCTATCTTT 
CACATTGCCA CTCTAATGCC CACCAAAGAC TTGGACAAAT ACCGCTGTGA CAAGAAGAGA 
CACCTGGGGA ATGACTTTGT ATCAATTATC TACAATGATT CTGGAGAAGA CTTCAAATTG 
GGCACCATCA AGGGCCAATT CAATTTTGTC CACGTGATAA TCACTCCTCT GGATTATGAA 
TGTAACCTGG TCACACTGCA GTGTCGGAAA GATATGGAAG GCCTCGTGGA TACCAGTGTG 
GCTAAAATTG TATCAGACCG AAACCTGCCC TTCGTTGCCC GCCAGATGGC ACTGCATGCG 
AATATGGCAT CGCAAGTTCA TCACAGCAGA TCAAATCCGA CAGACATCTA CCCCTCCAAG 
TGGATTGCCA GATTGCGTCA CATCAAACGA CTGCGGCATA GGATCCGGGA AGAAACCCAG 
TATCCGAACT CTGGCTTCCC CTTGATGCAG ATGCATCCAC CAGCTCACCC CAAGTCCCCA 
CCGCAGGCCC CATCCGACCC CACCCCTACC TATGAGACTG GTCAGAGGAA ACGGCTCATC 
TCTTCAGTGG ATGACTTTAC AGAGTTTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_007499379.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499441.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OMc28506
Clone ID Related Accession (Same CDS sequence)	XM_007499379.2
Accession Version	XM_007499379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGC AGGATACAGA CATCCATCAC TAGTTCTAGT 
TTGGGTTCTG CAGATGAGAA TTCAATGGCT CAGGCTGATG ATAACCTAAA AAACCTTCAC 
CTGGAGCTCA CAGAAACTTG CTTGGATATG ATGGCCAGAT ACGTCTTTTC CAACTTCACT 
GCTGTACCCA AGAGATCTCC TGTGGGAGAA TTTCTTCTTG CTGGAGGGAG AACCAAAACC 
TGGTTGGTTG GGAACAAGTT AATAACTGTA ACAACAAGTG TTGGTACTGG GACCAGATCC 
CTGCTGGGCC TGGATTCTGG GGACCTGCAG AATAATGAGT CAAGCACAGA TCCCAGTTTG 
CAAGTGAGAC AGACAAAGGA AGCACCGGCC AAGTTAGAGT CGCAGGCTGG CCAGCAAGTT 
TGCCGGGGGT CTCGAGACCG AGTTCGGTCT ATGTCAGGGG GCCATGCTCT CCGGGTCAAT 
GCCTTGGATA CTCCAGCCTC CCACTTCCCA AGCAATCCCA CTTCTCCAGG ATTACAGAAT 
ACACCTGCTG CCCGTACAGA GAAGACTTCA GCAAGCGCCC AGACCCCTTT ACAGAAGGGA 
AAGACCAACC TGGCTGCCTA TGTGCCCCTG CTCACCCAGG GCTGGGCGGA GATCCTGGTC 
CGAAGGCCCA CAGGGAATAC CAGCTGGCTG ATGAGCTTGG AAAACCCTCT TAGTCCGTTC 
TCTTCAGATA TCAATAACAT GCCACTCCAG GAGCTCTCTA ATGCATTAAT GGCAGCTGAG 
CGTTTTAAGG AGCATCGGGA GACTGCCCTG TACAAGTCCT TGTCAGTGCC ATCATCTAGC 
CTTACCACGG GGACAGACAA GCCTTCCCTC CTACAGCGTT CCAATACAGA GTCGGCAGTT 
GTTCTGGAAG AGGGAAGCCA AACAGAGGTG GACTTGAGAG AGACAGACTC GCCTTCCGAG 
TCTCAGGAAA TAGAGGATTT TGAAGCCATT GGCTCTCAGT CAACTGCAAG TGATGAGAGG 
TTTGGCAGAC TTCAGGATGT CTATAGTAGG TCCTCTTCCA CTTCAAGTCA AGAAGAGAAA 
TCCTTCCGCT CTGAAGAGCT CATTGCAGGT GGGATTCCCA TTGAGAGAGC TGTCTCTTCT 
GAAGATGGCA GGCCCATAGC TGAGCACTCC TTCCAGCCTT CTCAGCCCCT GAGCAAGTCC 
AGCTCTTCCC CTGAGTTACA GACCCTACAG GATGTCCTCA AGGATGCAGA GGATAAAGGC 
CATCTTGGGA AGCTGAGCAC AGATGTCAAA GCCAAGTCCC AGTCAGGGAA TTTAGAAGGA 
GAAGGAGCCA ACATCTGGCC AAGCCAAGGG GAGGAGAGCA GAGGACTTGG TGCAGGTCAG 
CAAGAGGCTG CAATGCCATC TTCAAGCTCG CCACGTTCCC CAAGTGGCCT CCGGCCTCGG 
GGCTACACTA TCTCTGACTC AGCCCCATCA AGGAGGGGCA AGAAGATGGA GAAGGATGCC 
TTCAAGAGTA GAGCTGCCAC TTCTAACACA GAGAAGGTGC CCGGGATTAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA TTCCCCTTTC TTTGGAGATG AGTCCAACAA GCCACTCCTC 
TTACCTCCTG AGACATTCGA AAGGTCCGTG CAGCTCCTCG ATCAGATTCC CTCTTATGAT 
ACCCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAACAGCGA GATCGCCATC 
CTTTCCAATG AGCATGGCTC CTATAGATAC ACAGAGTTCT TGACGGGCTT GGGGAAGCTC 
ATTGAACTCA AAGATTGCCA GCCAGACAAG ATCTACCTGG GGGGCCTGGA TGTGTGTGGG 
GAGGATGGGC AGTTCACCTA CTGCTGGCAC GATGATATCA TGCAAGCTAT CTTTCACATT 
GCCACTCTAA TGCCCACCAA AGACTTGGAC AAATACCGCT GTGACAAGAA GAGACACCTG 
GGGAATGACT TTGTATCAAT TATCTACAAT GATTCTGGAG AAGACTTCAA ATTGGGCACC 
ATCAAGGGCC AATTCAATTT TGTCCACGTG ATAATCACTC CTCTGGATTA TGAATGTAAC 
CTGGTCACAC TGCAGTGTCG GAAAGATATG GAAGGCCTCG TGGATACCAG TGTGGCTAAA 
ATTGTATCAG ACCGAAACCT GCCCTTCGTT GCCCGCCAGA TGGCACTGCA TGCGAATATG 
GCATCGCAAG TTCATCACAG CAGATCAAAT CCGACAGACA TCTACCCCTC CAAGTGGATT 
GCCAGATTGC GTCACATCAA ACGACTGCGG CATAGGATCC GGGAAGAAAC CCAGTATCCG 
AACTCTGGCT TCCCCTTGAT GCAGATGCAT CCACCAGCTC ACCCCAAGTC CCCACCGCAG 
GCCCCATCCG ACCCCACCCC TACCTATGAG ACTGGTCAGA GGAAACGGCT CATCTCTTCA 
GTGGATGACT TTACAGAGTT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499441.1
CDS	240..5666
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSSLRIARPPKQGLNNSPPVKEFKESSAVDAFRSRSISVSEHVVRSRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_007499379.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499379.2

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGTAG TTTAAGGATA GCCAGACCCC CCAAGCAAGG CTTGAATAAC 
TCTCCTCCCG TGAAGGAATT CAAAGAGAGC TCTGCAGTTG ATGCCTTCCG GTCCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTTCGCAGC AGGATACAGA CATCCATCAC TAGTTCTAGT 
TTGGGTTCTG CAGATGAGAA TTCAATGGCT CAGGCTGATG ATAACCTAAA AAACCTTCAC 
CTGGAGCTCA CAGAAACTTG CTTGGATATG ATGGCCAGAT ACGTCTTTTC CAACTTCACT 
GCTGTACCCA AGAGATCTCC TGTGGGAGAA TTTCTTCTTG CTGGAGGGAG AACCAAAACC 
TGGTTGGTTG GGAACAAGTT AATAACTGTA ACAACAAGTG TTGGTACTGG GACCAGATCC 
CTGCTGGGCC TGGATTCTGG GGACCTGCAG AATAATGAGT CAAGCACAGA TCCCAGTTTG 
CAAGTGAGAC AGACAAAGGA AGCACCGGCC AAGTTAGAGT CGCAGGCTGG CCAGCAAGTT 
TGCCGGGGGT CTCGAGACCG AGTTCGGTCT ATGTCAGGGG GCCATGCTCT CCGGGTCAAT 
GCCTTGGATA CTCCAGCCTC CCACTTCCCA AGCAATCCCA CTTCTCCAGG ATTACAGAAT 
ACACCTGCTG CCCGTACAGA GAAGACTTCA GCAAGCGCCC AGACCCCTTT ACAGAAGGGA 
AAGACCAACC TGGCTGCCTA TGTGCCCCTG CTCACCCAGG GCTGGGCGGA GATCCTGGTC 
CGAAGGCCCA CAGGGAATAC CAGCTGGCTG ATGAGCTTGG AAAACCCTCT TAGTCCGTTC 
TCTTCAGATA TCAATAACAT GCCACTCCAG GAGCTCTCTA ATGCATTAAT GGCAGCTGAG 
CGTTTTAAGG AGCATCGGGA GACTGCCCTG TACAAGTCCT TGTCAGTGCC ATCATCTAGC 
CTTACCACGG GGACAGACAA GCCTTCCCTC CTACAGCGTT CCAATACAGA GTCGGCAGTT 
GTTCTGGAAG AGGGAAGCCA AACAGAGGTG GACTTGAGAG AGACAGACTC GCCTTCCGAG 
TCTCAGGAAA TAGAGGATTT TGAAGCCATT GGCTCTCAGT CAACTGCAAG TGATGAGAGG 
TTTGGCAGAC TTCAGGATGT CTATAGTAGG TCCTCTTCCA CTTCAAGTCA AGAAGAGAAA 
TCCTTCCGCT CTGAAGAGCT CATTGCAGGT GGGATTCCCA TTGAGAGAGC TGTCTCTTCT 
GAAGATGGCA GGCCCATAGC TGAGCACTCC TTCCAGCCTT CTCAGCCCCT GAGCAAGTCC 
AGCTCTTCCC CTGAGTTACA GACCCTACAG GATGTCCTCA AGGATGCAGA GGATAAAGGC 
CATCTTGGGA AGCTGAGCAC AGATGTCAAA GCCAAGTCCC AGTCAGGGAA TTTAGAAGGA 
GAAGGAGCCA ACATCTGGCC AAGCCAAGGG GAGGAGAGCA GAGGACTTGG TGCAGGTCAG 
CAAGAGGCTG CAATGCCATC TTCAAGCTCG CCACGTTCCC CAAGTGGCCT CCGGCCTCGG 
GGCTACACTA TCTCTGACTC AGCCCCATCA AGGAGGGGCA AGAAGATGGA GAAGGATGCC 
TTCAAGAGTA GAGCTGCCAC TTCTAACACA GAGAAGGTGC CCGGGATTAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA TTCCCCTTTC TTTGGAGATG AGTCCAACAA GCCACTCCTC 
TTACCTCCTG AGACATTCGA AAGGTCCGTG CAGCTCCTCG ATCAGATTCC CTCTTATGAT 
ACCCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAACAGCGA GATCGCCATC 
CTTTCCAATG AGCATGGCTC CTATAGATAC ACAGAGTTCT TGACGGGCTT GGGGAAGCTC 
ATTGAACTCA AAGATTGCCA GCCAGACAAG ATCTACCTGG GGGGCCTGGA TGTGTGTGGG 
GAGGATGGGC AGTTCACCTA CTGCTGGCAC GATGATATCA TGCAAGCTAT CTTTCACATT 
GCCACTCTAA TGCCCACCAA AGACTTGGAC AAATACCGCT GTGACAAGAA GAGACACCTG 
GGGAATGACT TTGTATCAAT TATCTACAAT GATTCTGGAG AAGACTTCAA ATTGGGCACC 
ATCAAGGGCC AATTCAATTT TGTCCACGTG ATAATCACTC CTCTGGATTA TGAATGTAAC 
CTGGTCACAC TGCAGTGTCG GAAAGATATG GAAGGCCTCG TGGATACCAG TGTGGCTAAA 
ATTGTATCAG ACCGAAACCT GCCCTTCGTT GCCCGCCAGA TGGCACTGCA TGCGAATATG 
GCATCGCAAG TTCATCACAG CAGATCAAAT CCGACAGACA TCTACCCCTC CAAGTGGATT 
GCCAGATTGC GTCACATCAA ACGACTGCGG CATAGGATCC GGGAAGAAAC CCAGTATCCG 
AACTCTGGCT TCCCCTTGAT GCAGATGCAT CCACCAGCTC ACCCCAAGTC CCCACCGCAG 
GCCCCATCCG ACCCCACCCC TACCTATGAG ACTGGTCAGA GGAAACGGCT CATCTCTTCA 
GTGGATGACT TTACAGAGTT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_007499380.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499442.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OMc28507
Clone ID Related Accession (Same CDS sequence)	XM_007499380.2
Accession Version	XM_007499380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGCAG GATACAGACA TCCATCACTA GTTCTAGTTT GGGTTCTGCA 
GATGAGAATT CAATGGCTCA GGCTGATGAT AACCTAAAAA ACCTTCACCT GGAGCTCACA 
GAAACTTGCT TGGATATGAT GGCCAGATAC GTCTTTTCCA ACTTCACTGC TGTACCCAAG 
AGATCTCCTG TGGGAGAATT TCTTCTTGCT GGAGGGAGAA CCAAAACCTG GTTGGTTGGG 
AACAAGTTAA TAACTGTAAC AACAAGTGTT GGTACTGGGA CCAGATCCCT GCTGGGCCTG 
GATTCTGGGG ACCTGCAGAA TAATGAGTCA AGCACAGATC CCAGTTTGCA AGTGAGACAG 
ACAAAGGAAG CACCGGCCAA GTTAGAGTCG CAGGCTGGCC AGCAAGTTTG CCGGGGGTCT 
CGAGACCGAG TTCGGTCTAT GTCAGGGGGC CATGCTCTCC GGGTCAATGC CTTGGATACT 
CCAGCCTCCC ACTTCCCAAG CAATCCCACT TCTCCAGGAT TACAGAATAC ACCTGCTGCC 
CGTACAGAGA AGACTTCAGC AAGCGCCCAG ACCCCTTTAC AGAAGGGAAA GACCAACCTG 
GCTGCCTATG TGCCCCTGCT CACCCAGGGC TGGGCGGAGA TCCTGGTCCG AAGGCCCACA 
GGGAATACCA GCTGGCTGAT GAGCTTGGAA AACCCTCTTA GTCCGTTCTC TTCAGATATC 
AATAACATGC CACTCCAGGA GCTCTCTAAT GCATTAATGG CAGCTGAGCG TTTTAAGGAG 
CATCGGGAGA CTGCCCTGTA CAAGTCCTTG TCAGTGCCAT CATCTAGCCT TACCACGGGG 
ACAGACAAGC CTTCCCTCCT ACAGCGTTCC AATACAGTGG CCTCTTTCTC TTCCATGTAC 
CAGTCCAGTT GTCAAGGAAA GTTGCATAGG AGCGTTTCCT GGGCAGAGTC GGCAGTTGTT 
CTGGAAGAGG GAAGCCAAAC AGAGGTGGAC TTGAGAGAGA CAGACTCGCC TTCCGAGTCT 
CAGGAAATAG AGGATTTTGA AGCCATTGGC TCTCAGTCAA CTGCAAGTGA TGAGAGGTTT 
GGCAGACTTC AGGATGTCTA TAGTAGGTCC TCTTCCACTT CAAGTCAAGA AGAGAAATCC 
TTCCGCTCTG AAGAGCTCAT TGCAGGTGGG ATTCCCATTG AGAGAGCTGT CTCTTCTGAA 
GATGGCAGGC CCATAGCTGA GCACTCCTTC CAGCCTTCTC AGCCCCTGAG CAAGTCCAGC 
TCTTCCCCTG AGTTACAGAC CCTACAGGAT GTCCTCAAGG ATGCAGAGGA TAAAGGCCAT 
CTTGGGAAGC TGAGCACAGA TGTCAAAGCC AAGTCCCAGT CAGGGAATTT AGAAGGAGAA 
GGAGCCAACA TCTGGCCAAG CCAAGGGGAG GAGAGCAGAG GACTTGGTGC AGGTCAGCAA 
GAGGCTGCAA TGCCATCTTC AAGCTCGCCA CGTTCCCCAA GTGGCCTCCG GCCTCGGGGC 
TACACTATCT CTGACTCAGC CCCATCAAGG AGGGGCAAGA AGATGGAGAA GGATGCCTTC 
AAGAGTAGAG CTGCCACTTC TAACACAGAG AAGGTGCCCG GGATTAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCATTC CCCTTTCTTT GGAGATGAGT CCAACAAGCC ACTCCTCTTA 
CCTCCTGAGA CATTCGAAAG GTCCGTGCAG CTCCTCGATC AGATTCCCTC TTATGATACC 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA ACAGCGAGAT CGCCATCCTT 
TCCAATGAGC ATGGCTCCTA TAGATACACA GAGTTCTTGA CGGGCTTGGG GAAGCTCATT 
GAACTCAAAG ATTGCCAGCC AGACAAGATC TACCTGGGGG GCCTGGATGT GTGTGGGGAG 
GATGGGCAGT TCACCTACTG CTGGCACGAT GATATCATGC AAGCTATCTT TCACATTGCC 
ACTCTAATGC CCACCAAAGA CTTGGACAAA TACCGCTGTG ACAAGAAGAG ACACCTGGGG 
AATGACTTTG TATCAATTAT CTACAATGAT TCTGGAGAAG ACTTCAAATT GGGCACCATC 
AAGGGCCAAT TCAATTTTGT CCACGTGATA ATCACTCCTC TGGATTATGA ATGTAACCTG 
GTCACACTGC AGTGTCGGAA AGATATGGAA GGCCTCGTGG ATACCAGTGT GGCTAAAATT 
GTATCAGACC GAAACCTGCC CTTCGTTGCC CGCCAGATGG CACTGCATGC GAATATGGCA 
TCGCAAGTTC ATCACAGCAG ATCAAATCCG ACAGACATCT ACCCCTCCAA GTGGATTGCC 
AGATTGCGTC ACATCAAACG ACTGCGGCAT AGGATCCGGG AAGAAACCCA GTATCCGAAC 
TCTGGCTTCC CCTTGATGCA GATGCATCCA CCAGCTCACC CCAAGTCCCC ACCGCAGGCC 
CCATCCGACC CCACCCCTAC CTATGAGACT GGTCAGAGGA AACGGCTCAT CTCTTCAGTG 
GATGACTTTA CAGAGTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499442.1
CDS	240..5603
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTVASFSSMYQSSCQGKLHRSVSWAESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_007499380.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499380.2

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGCAG GATACAGACA TCCATCACTA GTTCTAGTTT GGGTTCTGCA 
GATGAGAATT CAATGGCTCA GGCTGATGAT AACCTAAAAA ACCTTCACCT GGAGCTCACA 
GAAACTTGCT TGGATATGAT GGCCAGATAC GTCTTTTCCA ACTTCACTGC TGTACCCAAG 
AGATCTCCTG TGGGAGAATT TCTTCTTGCT GGAGGGAGAA CCAAAACCTG GTTGGTTGGG 
AACAAGTTAA TAACTGTAAC AACAAGTGTT GGTACTGGGA CCAGATCCCT GCTGGGCCTG 
GATTCTGGGG ACCTGCAGAA TAATGAGTCA AGCACAGATC CCAGTTTGCA AGTGAGACAG 
ACAAAGGAAG CACCGGCCAA GTTAGAGTCG CAGGCTGGCC AGCAAGTTTG CCGGGGGTCT 
CGAGACCGAG TTCGGTCTAT GTCAGGGGGC CATGCTCTCC GGGTCAATGC CTTGGATACT 
CCAGCCTCCC ACTTCCCAAG CAATCCCACT TCTCCAGGAT TACAGAATAC ACCTGCTGCC 
CGTACAGAGA AGACTTCAGC AAGCGCCCAG ACCCCTTTAC AGAAGGGAAA GACCAACCTG 
GCTGCCTATG TGCCCCTGCT CACCCAGGGC TGGGCGGAGA TCCTGGTCCG AAGGCCCACA 
GGGAATACCA GCTGGCTGAT GAGCTTGGAA AACCCTCTTA GTCCGTTCTC TTCAGATATC 
AATAACATGC CACTCCAGGA GCTCTCTAAT GCATTAATGG CAGCTGAGCG TTTTAAGGAG 
CATCGGGAGA CTGCCCTGTA CAAGTCCTTG TCAGTGCCAT CATCTAGCCT TACCACGGGG 
ACAGACAAGC CTTCCCTCCT ACAGCGTTCC AATACAGTGG CCTCTTTCTC TTCCATGTAC 
CAGTCCAGTT GTCAAGGAAA GTTGCATAGG AGCGTTTCCT GGGCAGAGTC GGCAGTTGTT 
CTGGAAGAGG GAAGCCAAAC AGAGGTGGAC TTGAGAGAGA CAGACTCGCC TTCCGAGTCT 
CAGGAAATAG AGGATTTTGA AGCCATTGGC TCTCAGTCAA CTGCAAGTGA TGAGAGGTTT 
GGCAGACTTC AGGATGTCTA TAGTAGGTCC TCTTCCACTT CAAGTCAAGA AGAGAAATCC 
TTCCGCTCTG AAGAGCTCAT TGCAGGTGGG ATTCCCATTG AGAGAGCTGT CTCTTCTGAA 
GATGGCAGGC CCATAGCTGA GCACTCCTTC CAGCCTTCTC AGCCCCTGAG CAAGTCCAGC 
TCTTCCCCTG AGTTACAGAC CCTACAGGAT GTCCTCAAGG ATGCAGAGGA TAAAGGCCAT 
CTTGGGAAGC TGAGCACAGA TGTCAAAGCC AAGTCCCAGT CAGGGAATTT AGAAGGAGAA 
GGAGCCAACA TCTGGCCAAG CCAAGGGGAG GAGAGCAGAG GACTTGGTGC AGGTCAGCAA 
GAGGCTGCAA TGCCATCTTC AAGCTCGCCA CGTTCCCCAA GTGGCCTCCG GCCTCGGGGC 
TACACTATCT CTGACTCAGC CCCATCAAGG AGGGGCAAGA AGATGGAGAA GGATGCCTTC 
AAGAGTAGAG CTGCCACTTC TAACACAGAG AAGGTGCCCG GGATTAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCATTC CCCTTTCTTT GGAGATGAGT CCAACAAGCC ACTCCTCTTA 
CCTCCTGAGA CATTCGAAAG GTCCGTGCAG CTCCTCGATC AGATTCCCTC TTATGATACC 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA ACAGCGAGAT CGCCATCCTT 
TCCAATGAGC ATGGCTCCTA TAGATACACA GAGTTCTTGA CGGGCTTGGG GAAGCTCATT 
GAACTCAAAG ATTGCCAGCC AGACAAGATC TACCTGGGGG GCCTGGATGT GTGTGGGGAG 
GATGGGCAGT TCACCTACTG CTGGCACGAT GATATCATGC AAGCTATCTT TCACATTGCC 
ACTCTAATGC CCACCAAAGA CTTGGACAAA TACCGCTGTG ACAAGAAGAG ACACCTGGGG 
AATGACTTTG TATCAATTAT CTACAATGAT TCTGGAGAAG ACTTCAAATT GGGCACCATC 
AAGGGCCAAT TCAATTTTGT CCACGTGATA ATCACTCCTC TGGATTATGA ATGTAACCTG 
GTCACACTGC AGTGTCGGAA AGATATGGAA GGCCTCGTGG ATACCAGTGT GGCTAAAATT 
GTATCAGACC GAAACCTGCC CTTCGTTGCC CGCCAGATGG CACTGCATGC GAATATGGCA 
TCGCAAGTTC ATCACAGCAG ATCAAATCCG ACAGACATCT ACCCCTCCAA GTGGATTGCC 
AGATTGCGTC ACATCAAACG ACTGCGGCAT AGGATCCGGG AAGAAACCCA GTATCCGAAC 
TCTGGCTTCC CCTTGATGCA GATGCATCCA CCAGCTCACC CCAAGTCCCC ACCGCAGGCC 
CCATCCGACC CCACCCCTAC CTATGAGACT GGTCAGAGGA AACGGCTCAT CTCTTCAGTG 
GATGACTTTA CAGAGTTTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_007499381.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499443.1 Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OMc28508
Clone ID Related Accession (Same CDS sequence)	XM_007499381.2
Accession Version	XM_007499381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGGAT ACAGACATCC ATCACTAGTT CTAGTTTGGG TTCTGCAGAT 
GAGAATTCAA TGGCTCAGGC TGATGATAAC CTAAAAAACC TTCACCTGGA GCTCACAGAA 
ACTTGCTTGG ATATGATGGC CAGATACGTC TTTTCCAACT TCACTGCTGT ACCCAAGAGA 
TCTCCTGTGG GAGAATTTCT TCTTGCTGGA GGGAGAACCA AAACCTGGTT GGTTGGGAAC 
AAGTTAATAA CTGTAACAAC AAGTGTTGGT ACTGGGACCA GATCCCTGCT GGGCCTGGAT 
TCTGGGGACC TGCAGAATAA TGAGTCAAGC ACAGATCCCA GTTTGCAAGT GAGACAGACA 
AAGGAAGCAC CGGCCAAGTT AGAGTCGCAG GCTGGCCAGC AAGTTTGCCG GGGGTCTCGA 
GACCGAGTTC GGTCTATGTC AGGGGGCCAT GCTCTCCGGG TCAATGCCTT GGATACTCCA 
GCCTCCCACT TCCCAAGCAA TCCCACTTCT CCAGGATTAC AGAATACACC TGCTGCCCGT 
ACAGAGAAGA CTTCAGCAAG CGCCCAGACC CCTTTACAGA AGGGAAAGAC CAACCTGGCT 
GCCTATGTGC CCCTGCTCAC CCAGGGCTGG GCGGAGATCC TGGTCCGAAG GCCCACAGGG 
AATACCAGCT GGCTGATGAG CTTGGAAAAC CCTCTTAGTC CGTTCTCTTC AGATATCAAT 
AACATGCCAC TCCAGGAGCT CTCTAATGCA TTAATGGCAG CTGAGCGTTT TAAGGAGCAT 
CGGGAGACTG CCCTGTACAA GTCCTTGTCA GTGCCATCAT CTAGCCTTAC CACGGGGACA 
GACAAGCCTT CCCTCCTACA GCGTTCCAAT ACAGTGGCCT CTTTCTCTTC CATGTACCAG 
TCCAGTTGTC AAGGAAAGTT GCATAGGAGC GTTTCCTGGG CAGAGTCGGC AGTTGTTCTG 
GAAGAGGGAA GCCAAACAGA GGTGGACTTG AGAGAGACAG ACTCGCCTTC CGAGTCTCAG 
GAAATAGAGG ATTTTGAAGC CATTGGCTCT CAGTCAACTG CAAGTGATGA GAGGTTTGGC 
AGACTTCAGG ATGTCTATAG TAGGTCCTCT TCCACTTCAA GTCAAGAAGA GAAATCCTTC 
CGCTCTGAAG AGCTCATTGC AGGTGGGATT CCCATTGAGA GAGCTGTCTC TTCTGAAGAT 
GGCAGGCCCA TAGCTGAGCA CTCCTTCCAG CCTTCTCAGC CCCTGAGCAA GTCCAGCTCT 
TCCCCTGAGT TACAGACCCT ACAGGATGTC CTCAAGGATG CAGAGGATAA AGGCCATCTT 
GGGAAGCTGA GCACAGATGT CAAAGCCAAG TCCCAGTCAG GGAATTTAGA AGGAGAAGGA 
GCCAACATCT GGCCAAGCCA AGGGGAGGAG AGCAGAGGAC TTGGTGCAGG TCAGCAAGAG 
GCTGCAATGC CATCTTCAAG CTCGCCACGT TCCCCAAGTG GCCTCCGGCC TCGGGGCTAC 
ACTATCTCTG ACTCAGCCCC ATCAAGGAGG GGCAAGAAGA TGGAGAAGGA TGCCTTCAAG 
AGTAGAGCTG CCACTTCTAA CACAGAGAAG GTGCCCGGGA TTAACCCCAG CTTCGTATTC 
CTGCAGCTCT ACCATTCCCC TTTCTTTGGA GATGAGTCCA ACAAGCCACT CCTCTTACCT 
CCTGAGACAT TCGAAAGGTC CGTGCAGCTC CTCGATCAGA TTCCCTCTTA TGATACCCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GCGAGATCGC CATCCTTTCC 
AATGAGCATG GCTCCTATAG ATACACAGAG TTCTTGACGG GCTTGGGGAA GCTCATTGAA 
CTCAAAGATT GCCAGCCAGA CAAGATCTAC CTGGGGGGCC TGGATGTGTG TGGGGAGGAT 
GGGCAGTTCA CCTACTGCTG GCACGATGAT ATCATGCAAG CTATCTTTCA CATTGCCACT 
CTAATGCCCA CCAAAGACTT GGACAAATAC CGCTGTGACA AGAAGAGACA CCTGGGGAAT 
GACTTTGTAT CAATTATCTA CAATGATTCT GGAGAAGACT TCAAATTGGG CACCATCAAG 
GGCCAATTCA ATTTTGTCCA CGTGATAATC ACTCCTCTGG ATTATGAATG TAACCTGGTC 
ACACTGCAGT GTCGGAAAGA TATGGAAGGC CTCGTGGATA CCAGTGTGGC TAAAATTGTA 
TCAGACCGAA ACCTGCCCTT CGTTGCCCGC CAGATGGCAC TGCATGCGAA TATGGCATCG 
CAAGTTCATC ACAGCAGATC AAATCCGACA GACATCTACC CCTCCAAGTG GATTGCCAGA 
TTGCGTCACA TCAAACGACT GCGGCATAGG ATCCGGGAAG AAACCCAGTA TCCGAACTCT 
GGCTTCCCCT TGATGCAGAT GCATCCACCA GCTCACCCCA AGTCCCCACC GCAGGCCCCA 
TCCGACCCCA CCCCTACCTA TGAGACTGGT CAGAGGAAAC GGCTCATCTC TTCAGTGGAT 
GACTTTACAG AGTTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499443.1
CDS	240..5600
Translation	MAKPPSKDSGLKEKFRTLLGLGTPRPPRSSEGKQTEFIITAEILKELSVECGLNNRIRTIGQICEVAKTKKFEEHAVEAIWRAVADMLQPERPAEARHAVLYLLKAIIQGQGERLGVLRAHLFKVIKNYPSNEDLHERLEVFKALTDNGRYITYLEEELADFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTASSVDIEVSLQVLDAVVCYNCLPSESLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLVKKYRKELQAVTWDILLKIIERLLQQLQSIESQELKTIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNETRSAVRIKVLDVLSFVLSINSQFYEEELINMVVISQLAHIPEDKDHQVRKLATQLLVDLAECCHTHHFNSLLDIIEKVIARSLSPPPELEDRDLASYSASLEDVKTAVLGLLVILQTKLYTLPASHASRVYEMLVNHIQLHYKYMYKLPIASSIRLKAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDNIEPERGSEKKASGGTLSPPTGPPSIAAQGASVRLGYLPYSRFFGVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSSCNVDQLSSALCSMLSDKKTPERLSGTPDAFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEHGLIYRCANQCVVALSVCSVEMPDVIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLITVTTSVGTGTRSLLGLDSGDLQNNESSTDPSLQVRQTKEAPAKLESQAGQQVCRGSRDRVRSMSGGHALRVNALDTPASHFPSNPTSPGLQNTPAARTEKTSASAQTPLQKGKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRETALYKSLSVPSSSLTTGTDKPSLLQRSNTVASFSSMYQSSCQGKLHRSVSWAESAVVLEEGSQTEVDLRETDSPSESQEIEDFEAIGSQSTASDERFGRLQDVYSRSSSTSSQEEKSFRSEELIAGGIPIERAVSSEDGRPIAEHSFQPSQPLSKSSSSPELQTLQDVLKDAEDKGHLGKLSTDVKAKSQSGNLEGEGANIWPSQGEESRGLGAGQQEAAMPSSSSPRSPSGLRPRGYTISDSAPSRRGKKMEKDAFKSRAATSNTEKVPGINPSFVFLQLYHSPFFGDESNKPLLLPPETFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVTLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREETQYPNSGFPLMQMHPPAHPKSPPQAPSDPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_007499381.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499381.2

ORF Insert Sequence:

ATGGCTAAGC CACCAAGCAA AGATTCTGGG CTAAAGGAGA AGTTCAGGAC TCTGTTGGGC 
CTGGGAACAC CAAGACCTCC CAGGTCTTCT GAGGGCAAAC AAACTGAGTT TATCATCACT 
GCTGAAATTT TAAAGGAACT CAGTGTAGAA TGTGGTTTGA ATAATCGGAT TCGGACGATA 
GGTCAAATCT GTGAAGTAGC AAAAACCAAG AAATTTGAAG AGCATGCAGT GGAAGCTATC 
TGGAGAGCTG TAGCTGACAT GCTTCAGCCA GAGAGGCCAG CAGAAGCCCG GCATGCAGTT 
CTGTATCTGC TGAAGGCCAT TATTCAGGGT CAGGGGGAAC GCTTGGGTGT TCTTAGAGCA 
CATCTCTTTA AGGTAATCAA GAATTACCCT TCCAATGAAG ACCTGCATGA GAGACTTGAA 
GTTTTCAAGG CTCTCACTGA CAATGGGAGA TACATAACAT ACTTGGAAGA AGAACTGGCT 
GACTTTGTCC TGCAGTGGAT GGATATTGGC TTATCGTCAG AATTTCTTCT GGTGCTCGTG 
AACCTGGTCA AATTTAATAG CTGTTACCTA GATGAATATA TTGCTTCAAT GGTCCACATG 
ATCTGTTTGC TTTGCATTCG TACTGCATCT TCTGTGGACA TAGAGGTCTC CTTGCAGGTC 
CTAGATGCTG TGGTTTGTTA TAATTGCCTT CCCTCAGAGA GCCTCCCCGT GTTTATCATC 
ACACTGTGCC GGACCATCAA TGTCAAAGAG CTCTGTGAGC CCTGCTGGAA GCTCATGCGT 
AATCTTCTGG GAACCCATTT AGGCCACAGT GCCATATACA ACATGTGTCG TATCATGGAG 
GACAGAGCCT ACATGGAAGA TGCCCCTTTG CTGAGAGGAG CAGTATTCTT TGTTGGGATG 
GCTCTCTGGG GTGCTCACAG ACTGTATTCA CTCAAGAATT CACCTACGTC AGTATTGCCT 
TCTTTTTATG AGGCCATGAC TTGTCCCAAT GAGGTGGTTT CCTATGAGAT AGTCCTGTCC 
ATCACCAGGC TTGTGAAGAA GTACAGGAAG GAGCTACAAG CAGTTACTTG GGACATTCTT 
CTAAAGATTA TTGAGCGACT ATTACAACAG TTGCAGAGTA TAGAAAGCCA AGAGCTCAAA 
ACCATTGTTC ATGATTTATT GACCACTGTG GAAGAGCTCT GTGATCAGAA TGAGTTCCAT 
GGCTCAGAGG AGAGATTCTT TGAACTTGTC GAGAGATGTG CAGATCAGCG GCCGGAATCC 
TCTCTTTTAA ACTTAATAAC GTATAGAGCT CAATCTATTC ATCCTGCCAA AGATGGCTGG 
ATTCATAACT TACAGTTATT AATGGAGAGA TTCTTCAGGA ATGAGACCCG CAGTGCTGTG 
CGCATTAAAG TACTCGACGT TTTATCCTTT GTTCTGAGTA TTAACAGCCA ATTTTATGAG 
GAGGAGCTGA TTAACATGGT AGTCATCTCA CAACTAGCCC ACATTCCTGA GGACAAAGAC 
CACCAAGTCC GAAAACTAGC TACTCAGCTG CTGGTGGACC TGGCAGAATG CTGTCACACT 
CATCACTTTA ACAGCTTGCT GGATATCATT GAAAAGGTGA TTGCACGTTC ACTTTCTCCT 
CCTCCTGAAT TGGAAGATCG GGACTTAGCC TCATATTCAG CTTCCTTAGA AGATGTGAAA 
ACTGCTGTAC TTGGCCTTCT GGTTATTCTT CAGACCAAGC TCTATACCTT GCCTGCCAGT 
CATGCCTCCA GAGTGTATGA GATGCTGGTC AATCACATTC AACTGCATTA CAAGTACATG 
TATAAGCTTC CAATCGCCAG CAGCATCAGG CTTAAGGCCT TTGACTTCTT GCTGCTGCTC 
CGAGCTGATT CACTCCATCG TCTTGGCCTT CCCAATAAAG ATGGTGTTGT GAGATTTAGC 
CCCTATTGCC TCTGTGACAA CATAGAACCT GAGAGAGGCT CTGAAAAGAA GGCCAGTGGT 
GGTACCCTCT CTCCACCCAC AGGGCCTCCT AGCATTGCTG CGCAAGGTGC ATCTGTCCGC 
TTGGGCTATT TGCCCTATTC CCGATTTTTT GGAGTGCTAC TTCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AAGTATTGAA ACTGGTTCTC AGTAAGCTGC CTGAGTCCCT CCGATATAAA 
GTTCTCATTT TCACTTCTTC TTGCAATGTA GACCAGCTGT CATCTGCTCT CTGCTCAATG 
CTTTCGGATA AAAAGACTCC TGAGCGACTC TCTGGCACAC CCGATGCATT CTCTCGAACA 
GACTTACATT TGGCAGTGGT TCCAGTACTG ACAGCTTTGA TATCCTACCA TAACTATTTG 
GATAAAACTA AACAGCGGGA GATGGTGTAT TGCCTGGAGC ATGGTCTCAT TTATCGGTGT 
GCTAACCAGT GTGTTGTGGC ATTGTCAGTG TGCAGTGTAG AAATGCCAGA TGTTATCATC 
AAAGCACTGC CAGTTCTGAT TGTCAAGCTC ACCCACATTT CTGCAACAGC CAACATGGCT 
ATCCCACTTC TGGAATTCCT TTCGACTTTA GCCAGATTGC CTCACCTCTA CAGGAACTTT 
GCTGCCGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CATACACAAA CCCTTCCAAA 
TTCAATCAGT ATATCGTATG CCTGGCTCAT CATGTTATAG CCATGTGGTT CATCAGATGC 
CGCCTGCCCT TTCGAAAAGA CTTTGTTCCC TACATCACCA AGGGCTTACG CTCCAATGTT 
CTCCTGTCTT TTGATGACAC CCCTGAAAAG GACAGCTTCA GGGCTCGGAG CACAAGCCTT 
AATGAGAGAC CAAAAAGGAT ACAGACATCC ATCACTAGTT CTAGTTTGGG TTCTGCAGAT 
GAGAATTCAA TGGCTCAGGC TGATGATAAC CTAAAAAACC TTCACCTGGA GCTCACAGAA 
ACTTGCTTGG ATATGATGGC CAGATACGTC TTTTCCAACT TCACTGCTGT ACCCAAGAGA 
TCTCCTGTGG GAGAATTTCT TCTTGCTGGA GGGAGAACCA AAACCTGGTT GGTTGGGAAC 
AAGTTAATAA CTGTAACAAC AAGTGTTGGT ACTGGGACCA GATCCCTGCT GGGCCTGGAT 
TCTGGGGACC TGCAGAATAA TGAGTCAAGC ACAGATCCCA GTTTGCAAGT GAGACAGACA 
AAGGAAGCAC CGGCCAAGTT AGAGTCGCAG GCTGGCCAGC AAGTTTGCCG GGGGTCTCGA 
GACCGAGTTC GGTCTATGTC AGGGGGCCAT GCTCTCCGGG TCAATGCCTT GGATACTCCA 
GCCTCCCACT TCCCAAGCAA TCCCACTTCT CCAGGATTAC AGAATACACC TGCTGCCCGT 
ACAGAGAAGA CTTCAGCAAG CGCCCAGACC CCTTTACAGA AGGGAAAGAC CAACCTGGCT 
GCCTATGTGC CCCTGCTCAC CCAGGGCTGG GCGGAGATCC TGGTCCGAAG GCCCACAGGG 
AATACCAGCT GGCTGATGAG CTTGGAAAAC CCTCTTAGTC CGTTCTCTTC AGATATCAAT 
AACATGCCAC TCCAGGAGCT CTCTAATGCA TTAATGGCAG CTGAGCGTTT TAAGGAGCAT 
CGGGAGACTG CCCTGTACAA GTCCTTGTCA GTGCCATCAT CTAGCCTTAC CACGGGGACA 
GACAAGCCTT CCCTCCTACA GCGTTCCAAT ACAGTGGCCT CTTTCTCTTC CATGTACCAG 
TCCAGTTGTC AAGGAAAGTT GCATAGGAGC GTTTCCTGGG CAGAGTCGGC AGTTGTTCTG 
GAAGAGGGAA GCCAAACAGA GGTGGACTTG AGAGAGACAG ACTCGCCTTC CGAGTCTCAG 
GAAATAGAGG ATTTTGAAGC CATTGGCTCT CAGTCAACTG CAAGTGATGA GAGGTTTGGC 
AGACTTCAGG ATGTCTATAG TAGGTCCTCT TCCACTTCAA GTCAAGAAGA GAAATCCTTC 
CGCTCTGAAG AGCTCATTGC AGGTGGGATT CCCATTGAGA GAGCTGTCTC TTCTGAAGAT 
GGCAGGCCCA TAGCTGAGCA CTCCTTCCAG CCTTCTCAGC CCCTGAGCAA GTCCAGCTCT 
TCCCCTGAGT TACAGACCCT ACAGGATGTC CTCAAGGATG CAGAGGATAA AGGCCATCTT 
GGGAAGCTGA GCACAGATGT CAAAGCCAAG TCCCAGTCAG GGAATTTAGA AGGAGAAGGA 
GCCAACATCT GGCCAAGCCA AGGGGAGGAG AGCAGAGGAC TTGGTGCAGG TCAGCAAGAG 
GCTGCAATGC CATCTTCAAG CTCGCCACGT TCCCCAAGTG GCCTCCGGCC TCGGGGCTAC 
ACTATCTCTG ACTCAGCCCC ATCAAGGAGG GGCAAGAAGA TGGAGAAGGA TGCCTTCAAG 
AGTAGAGCTG CCACTTCTAA CACAGAGAAG GTGCCCGGGA TTAACCCCAG CTTCGTATTC 
CTGCAGCTCT ACCATTCCCC TTTCTTTGGA GATGAGTCCA ACAAGCCACT CCTCTTACCT 
CCTGAGACAT TCGAAAGGTC CGTGCAGCTC CTCGATCAGA TTCCCTCTTA TGATACCCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GCGAGATCGC CATCCTTTCC 
AATGAGCATG GCTCCTATAG ATACACAGAG TTCTTGACGG GCTTGGGGAA GCTCATTGAA 
CTCAAAGATT GCCAGCCAGA CAAGATCTAC CTGGGGGGCC TGGATGTGTG TGGGGAGGAT 
GGGCAGTTCA CCTACTGCTG GCACGATGAT ATCATGCAAG CTATCTTTCA CATTGCCACT 
CTAATGCCCA CCAAAGACTT GGACAAATAC CGCTGTGACA AGAAGAGACA CCTGGGGAAT 
GACTTTGTAT CAATTATCTA CAATGATTCT GGAGAAGACT TCAAATTGGG CACCATCAAG 
GGCCAATTCA ATTTTGTCCA CGTGATAATC ACTCCTCTGG ATTATGAATG TAACCTGGTC 
ACACTGCAGT GTCGGAAAGA TATGGAAGGC CTCGTGGATA CCAGTGTGGC TAAAATTGTA 
TCAGACCGAA ACCTGCCCTT CGTTGCCCGC CAGATGGCAC TGCATGCGAA TATGGCATCG 
CAAGTTCATC ACAGCAGATC AAATCCGACA GACATCTACC CCTCCAAGTG GATTGCCAGA 
TTGCGTCACA TCAAACGACT GCGGCATAGG ATCCGGGAAG AAACCCAGTA TCCGAACTCT 
GGCTTCCCCT TGATGCAGAT GCATCCACCA GCTCACCCCA AGTCCCCACC GCAGGCCCCA 
TCCGACCCCA CCCCTACCTA TGAGACTGGT CAGAGGAAAC GGCTCATCTC TTCAGTGGAT 
GACTTTACAG AGTTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TSC2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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