ECU10_0250 cDNA ORF clone, Encephalitozoon cuniculi GB-M1

Gene
Clones
gRNAs

Summary

Gene Symbol	ECU10_0250
Entrez Gene ID	859788
Full Name	DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT
Gene Type	protein-coding
Organism	Encephalitozoon cuniculi GB-M1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001041973.1	NP_586140.1	DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.
Katinka MD, Duprat S, Cornillot E, Méténier G, Thomarat F, Prensier G, Barbe V, Peyretaillade E, Brottier P, Wincker P, Delbac F, El Alaoui H, Peyret P, Saurin W, Gouy M, Weissenbach J, Vivarès CP
Nature414(6862)450-3(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ECU10_0250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECU10_0250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEd01204	NM_001041973.1 Latest version!	Encephalitozoon cuniculi GB-M1 DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT (ECU10_0250) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECU10_0250 ( NM_001041973.1 ) cDNA ORF clone, Encephalitozoon cuniculi GB-M1 -> NP_586140.1 Encephalitozoon cuniculi GB-M1 DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT (ECU10_0250) partial mRNA.

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CloneID	OEd01204
Clone ID Related Accession (Same CDS sequence)	NM_001041973.1
Accession Version	NM_001041973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1149436800000
Organism	Encephalitozoon cuniculi GB-M1
Product	DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003236). COMPLETENESS: incomplete on both ends.

ATGGAGTCTG CTTTCACAAA AGAGCATAGC TGGTCGATCA TATCCTCTTT CTTTGAGCAA 
AAAGGACTTG TTAGACAGCA GCTCGACTCG TTTGATCAGT TTGTTAAAAC AAAGATGCAG 
GAGGTTATTA ATGAAAGCCC GGTAATAATC GTCCAGTCAG CACCCACAGC AGGGATAGAG 
ACTCAAAAGA GAATAGCCGT TAGATTTGGA CAGATATATG TCTCCAAACC ACCTGTCTAC 
ACCGAGTCTG ACGGAAGGAC GATCACAGTC TTTCCGAATG AAGCCCGCAT AAGAGACTTA 
ACATATGCAT CTCCTCTGTT TATCGATGTT ACAAAAGAGA CTTTGTCTGA GCTGGGAGTT 
GTTGACAAGC ACAAATACTC AAGGGTTCCC TTTGGATCGT TGCCTGTGAT GCTGAGGTCA 
TCATACTGTG TTCTCTATGG ACTAGGCGAT AAGGACCTCA TTGACCTTGG AGAATGCCCG 
TATGATCAAG GGGGGTACTT CATAGTGAAT GGATCTGAAA AGGTCATTGT GGCCCAGGAA 
CGAATGGCAA GTAACACTGT CTATGTGTTC AAGAAGGCAC AGCCTGCGAC CTATACACAC 
TATGCAGAAA TACGGTCTGT TCCTGAAAAG AGCTCCAGAA ACCCAAGCAC CCTGTCTATG 
AAGCTTTGTA GATCCCCGGG GGTGATAAGG GTTTCACTGC CATTGGTTAA GCAAGACGTC 
CCTCTTTTTG TTTTGTACAG GGCGTTGGGG TTTTTGTCAG ACAAGGAGAT AATTGATCAT 
ATTCTTTACG AAGACGATGA GGAGATGTTT GAGCTCCTGA AAGAAAGTAT TGAGGAGGGA 
ACCGTTGTTC AGGACCAAAA TGTGGCGCTA GACTACATCG GAAAGAGGTC TGCGCCTATA 
GGAACGCCAC AGGAGAAAAG AATAGTGATG GCCAAGGACC TTCTTGCAAA GGAAGTCCTC 
CCCCACATTG GAACACAGGA GTTTTGTGAA ACCAAAAAGG CCTACTTTAT TGGATATATT 
GTCCAGAGGC TCTTGCTTGT GGCGTTAGGA AGAAGAAACC CCGACGACAG AGATCATTAC 
GGAAAGAAAA GAATGGACCT CAGCGGGCCT CTACTTGCAT CACTCTTCAG AACCCTGTTT 
AAGAAGCTGT GTGTGGATAC AACGAGACAC ATGCAAAAGT GCATAGAGAA TGGAAGAGAG 
TTCAATATAG CTCTTGGACT GAAGGCAAGC ATAATTACAC AGGGGTTTCG GTACGCATTG 
GCAACAGGGA ACTGGGGAGA CCAGGCAAAG GCGATGCAGA CGAGGGCTGG GGTTGCTCAG 
GTTCTGAATC GATACAACTT TGTTTCGACT CTTAGCCATC TGAGGAGAGT CAACACACCC 
ATTGAAAAGG AAGGAAAGTT GGCAGCGCCC CGACAGCTCC ATAACACACA CTGGGGTATG 
GTTTGTCCTG CGGAGACGCC CGAGGGCCAG GCGTGTGGCC TCGTAAAGAA CCTGTCACTC 
ATGGCGTATA TCTCTGTTGG GTCATCTTCT GGGCCATTGG TTGAGTTTTT AGAAGAGTGT 
GGTGTTGAAT CATTGGAGGA GATATCCACT TCACAGCTGG ATGCAGCAAC CAAGATCTTT 
GTGAATGGAG TTTGGGTTGG GATTCATTCA GATCCTGTGG GTCTGATAAA ATCGCTCAAG 
TTATTGAGGC GAAGCCTTGA GATGGATAAG GAGGTTAGCA TCGTCCGAGA TATTCGGGAA 
AAAGAAATCA GGGTACAGTC AGATGCTGGA AGGCCTTGTA GGCCTCTACT GGTTGTCAAA 
GATAATAAGC TTGTCATTAC CGCAGAAGAC ATAAGAAAAT TGAAAAGGGG AGAAATTAAA 
TGGGATAACT TGGTTACCTC GGGGTTTATT GAGTTTCTGG ATGTTGAGGA GGAAGAAATG 
TCTATGATAG CGATGAACAC GAAGATTCTT GCCGACGAGT CTAGGAACTC GTCTGACGTG 
AGTGTTTCCT ATACTCACTG TGAGATCCAT CCAGCATTGA TATTGGGGAT TTGTGCCTCA 
ACCATTCCGT TTCCTGACCA CAATCAGAGT CCTCGTAACA CATACCAGAG TGCAATGGGC 
AAGCAAGCGA TGGGGATCTA TGCAACAAAC TTTCTGCTGA GGATGGACAC GCTGAGCAAT 
ATCCTGTTCT ATCCTCAGAA GCCGCTTGTG ACAACAAAAA GTATGGAATA TCTTCGGTTC 
AAGGAGCTTC CATCCGGCCA GAATGCTCTG GTTGCAATTG CGTGCTATTC TGGATACAAT 
CAGGAGGATA GCATTATCAT GAATCAGAGT GCCATTGATA GAGGCTTGTT TAGAAGTTTC 
TTCTATAGGA CATACACCGA CCAGGAGTCT ATGTCAAGGC CTGGTGTCAA CGAGGAGTTT 
TGCAAGCCTA GCAGAGGGGC TGTTCTGAGG ATGAAGAACC TGAATTACAA CAAGCTGGAT 
GATGATGGAC TTATAAGCCC CGGGACAAGG GTTACTGGGG ACGATGTTCT TATTGGAAAG 
ATAACGCCCA TACTGGACCC TGAAAGAAGT ACCAAAGAGG CGCCTGTTTA TGTGTACAAG 
GACAGCAGCA CTGCAATGCG TAGAACGGAA ACAGGAATTG TTGACACGGT TATTGTTACA 
AACAAGGATG GATACAAGTT TTCCAAGGTC AAGGTAAGGT CTGGGAGGAT TCCCCAGATG 
GGAGACAAGT TTGCGTCTAG ACATGCGCAG AAGGGAACAA TAGGAATTAC TTTGAGGCAG 
GAAGACATGC CCTTTACTTC TGAAGGGATA GTTCCGGACA TAATCATAAA CCCGCATGCG 
ATTCCCAGCC GTATGACCAT CGGGCATTTG ATTGAATGTC TCCTGGGCAA GGTTAGTGCA 
ATGAGTGGAG AGGAGGGGGA TGCAACACCA TTTAGTGGCG TGACTGTTGA CGGGATATCC 
TCCAGGTTGA AGTCGTATGG CTTCCAGCAA AGGGGGTTGG AGGTTATGTA CAATGGAATG 
ACAGGGAGAA AGCTCCGAGC TCAGATGTTC TTTGGGCCGA CATATTACCA ACGGCTTAAG 
CATATGGTTG ACGATAAGAT ACATGCTAGA GCTCGAGGCC CGCTCCAGAT ACTCACCAGG 
CAGCCTGTTG AGGGAAGGTC CAGGGATGGT GGGCTCCGGT TTGGAGAAAT GGAAAGAGAC 
TGTATAATTT CCCACGGGGC ATCTGCTTTT CTGAAAGAAA GGCTGATGGA TGTGAGCGAT 
GCATACTCTT GCTATGTGTG TGACTTTTGC GGGCTTCTTG CAATGGGTGG AAGCAAGGTG 
AATGAATGCA AGGGATGTAA CAACACCACG AATGTGAGCA TGGTTGAGAT TCCGTATGCC 
TTCAAACTCT TGATTCAGGA GCTGATGGGA ATGAATATCG CACCAAGGAT TCGCTTTGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_586140.1
CDS	1..3426
Translation	MESAFTKEHSWSIISSFFEQKGLVRQQLDSFDQFVKTKMQEVINESPVIIVQSAPTAGIETQKRIAVRFGQIYVSKPPVYTESDGRTITVFPNEARIRDLTYASPLFIDVTKETLSELGVVDKHKYSRVPFGSLPVMLRSSYCVLYGLGDKDLIDLGECPYDQGGYFIVNGSEKVIVAQERMASNTVYVFKKAQPATYTHYAEIRSVPEKSSRNPSTLSMKLCRSPGVIRVSLPLVKQDVPLFVLYRALGFLSDKEIIDHILYEDDEEMFELLKESIEEGTVVQDQNVALDYIGKRSAPIGTPQEKRIVMAKDLLAKEVLPHIGTQEFCETKKAYFIGYIVQRLLLVALGRRNPDDRDHYGKKRMDLSGPLLASLFRTLFKKLCVDTTRHMQKCIENGREFNIALGLKASIITQGFRYALATGNWGDQAKAMQTRAGVAQVLNRYNFVSTLSHLRRVNTPIEKEGKLAAPRQLHNTHWGMVCPAETPEGQACGLVKNLSLMAYISVGSSSGPLVEFLEECGVESLEEISTSQLDAATKIFVNGVWVGIHSDPVGLIKSLKLLRRSLEMDKEVSIVRDIREKEIRVQSDAGRPCRPLLVVKDNKLVITAEDIRKLKRGEIKWDNLVTSGFIEFLDVEEEEMSMIAMNTKILADESRNSSDVSVSYTHCEIHPALILGICASTIPFPDHNQSPRNTYQSAMGKQAMGIYATNFLLRMDTLSNILFYPQKPLVTTKSMEYLRFKELPSGQNALVAIACYSGYNQEDSIIMNQSAIDRGLFRSFFYRTYTDQESMSRPGVNEEFCKPSRGAVLRMKNLNYNKLDDDGLISPGTRVTGDDVLIGKITPILDPERSTKEAPVYVYKDSSTAMRRTETGIVDTVIVTNKDGYKFSKVKVRSGRIPQMGDKFASRHAQKGTIGITLRQEDMPFTSEGIVPDIIINPHAIPSRMTIGHLIECLLGKVSAMSGEEGDATPFSGVTVDGISSRLKSYGFQQRGLEVMYNGMTGRKLRAQMFFGPTYYQRLKHMVDDKIHARARGPLQILTRQPVEGRSRDGGLRFGEMERDCIISHGASAFLKERLMDVSDAYSCYVCDFCGLLAMGGSKVNECKGCNNTTNVSMVEIPYAFKLLIQELMGMNIAPRIRFEE

Target ORF information:

RefSeq Version	NM_001041973.1
Organism	Encephalitozoon cuniculi GB-M1
Definition	Encephalitozoon cuniculi GB-M1 DNA-DIRECTED RNA POLYMERASE II SECOND LARGEST SUBUNIT (ECU10_0250) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001041973.1

ORF Insert Sequence:

ATGGAGTCTG CTTTCACAAA AGAGCATAGC TGGTCGATCA TATCCTCTTT CTTTGAGCAA 
AAAGGACTTG TTAGACAGCA GCTCGACTCG TTTGATCAGT TTGTTAAAAC AAAGATGCAG 
GAGGTTATTA ATGAAAGCCC GGTAATAATC GTCCAGTCAG CACCCACAGC AGGGATAGAG 
ACTCAAAAGA GAATAGCCGT TAGATTTGGA CAGATATATG TCTCCAAACC ACCTGTCTAC 
ACCGAGTCTG ACGGAAGGAC GATCACAGTC TTTCCGAATG AAGCCCGCAT AAGAGACTTA 
ACATATGCAT CTCCTCTGTT TATCGATGTT ACAAAAGAGA CTTTGTCTGA GCTGGGAGTT 
GTTGACAAGC ACAAATACTC AAGGGTTCCC TTTGGATCGT TGCCTGTGAT GCTGAGGTCA 
TCATACTGTG TTCTCTATGG ACTAGGCGAT AAGGACCTCA TTGACCTTGG AGAATGCCCG 
TATGATCAAG GGGGGTACTT CATAGTGAAT GGATCTGAAA AGGTCATTGT GGCCCAGGAA 
CGAATGGCAA GTAACACTGT CTATGTGTTC AAGAAGGCAC AGCCTGCGAC CTATACACAC 
TATGCAGAAA TACGGTCTGT TCCTGAAAAG AGCTCCAGAA ACCCAAGCAC CCTGTCTATG 
AAGCTTTGTA GATCCCCGGG GGTGATAAGG GTTTCACTGC CATTGGTTAA GCAAGACGTC 
CCTCTTTTTG TTTTGTACAG GGCGTTGGGG TTTTTGTCAG ACAAGGAGAT AATTGATCAT 
ATTCTTTACG AAGACGATGA GGAGATGTTT GAGCTCCTGA AAGAAAGTAT TGAGGAGGGA 
ACCGTTGTTC AGGACCAAAA TGTGGCGCTA GACTACATCG GAAAGAGGTC TGCGCCTATA 
GGAACGCCAC AGGAGAAAAG AATAGTGATG GCCAAGGACC TTCTTGCAAA GGAAGTCCTC 
CCCCACATTG GAACACAGGA GTTTTGTGAA ACCAAAAAGG CCTACTTTAT TGGATATATT 
GTCCAGAGGC TCTTGCTTGT GGCGTTAGGA AGAAGAAACC CCGACGACAG AGATCATTAC 
GGAAAGAAAA GAATGGACCT CAGCGGGCCT CTACTTGCAT CACTCTTCAG AACCCTGTTT 
AAGAAGCTGT GTGTGGATAC AACGAGACAC ATGCAAAAGT GCATAGAGAA TGGAAGAGAG 
TTCAATATAG CTCTTGGACT GAAGGCAAGC ATAATTACAC AGGGGTTTCG GTACGCATTG 
GCAACAGGGA ACTGGGGAGA CCAGGCAAAG GCGATGCAGA CGAGGGCTGG GGTTGCTCAG 
GTTCTGAATC GATACAACTT TGTTTCGACT CTTAGCCATC TGAGGAGAGT CAACACACCC 
ATTGAAAAGG AAGGAAAGTT GGCAGCGCCC CGACAGCTCC ATAACACACA CTGGGGTATG 
GTTTGTCCTG CGGAGACGCC CGAGGGCCAG GCGTGTGGCC TCGTAAAGAA CCTGTCACTC 
ATGGCGTATA TCTCTGTTGG GTCATCTTCT GGGCCATTGG TTGAGTTTTT AGAAGAGTGT 
GGTGTTGAAT CATTGGAGGA GATATCCACT TCACAGCTGG ATGCAGCAAC CAAGATCTTT 
GTGAATGGAG TTTGGGTTGG GATTCATTCA GATCCTGTGG GTCTGATAAA ATCGCTCAAG 
TTATTGAGGC GAAGCCTTGA GATGGATAAG GAGGTTAGCA TCGTCCGAGA TATTCGGGAA 
AAAGAAATCA GGGTACAGTC AGATGCTGGA AGGCCTTGTA GGCCTCTACT GGTTGTCAAA 
GATAATAAGC TTGTCATTAC CGCAGAAGAC ATAAGAAAAT TGAAAAGGGG AGAAATTAAA 
TGGGATAACT TGGTTACCTC GGGGTTTATT GAGTTTCTGG ATGTTGAGGA GGAAGAAATG 
TCTATGATAG CGATGAACAC GAAGATTCTT GCCGACGAGT CTAGGAACTC GTCTGACGTG 
AGTGTTTCCT ATACTCACTG TGAGATCCAT CCAGCATTGA TATTGGGGAT TTGTGCCTCA 
ACCATTCCGT TTCCTGACCA CAATCAGAGT CCTCGTAACA CATACCAGAG TGCAATGGGC 
AAGCAAGCGA TGGGGATCTA TGCAACAAAC TTTCTGCTGA GGATGGACAC GCTGAGCAAT 
ATCCTGTTCT ATCCTCAGAA GCCGCTTGTG ACAACAAAAA GTATGGAATA TCTTCGGTTC 
AAGGAGCTTC CATCCGGCCA GAATGCTCTG GTTGCAATTG CGTGCTATTC TGGATACAAT 
CAGGAGGATA GCATTATCAT GAATCAGAGT GCCATTGATA GAGGCTTGTT TAGAAGTTTC 
TTCTATAGGA CATACACCGA CCAGGAGTCT ATGTCAAGGC CTGGTGTCAA CGAGGAGTTT 
TGCAAGCCTA GCAGAGGGGC TGTTCTGAGG ATGAAGAACC TGAATTACAA CAAGCTGGAT 
GATGATGGAC TTATAAGCCC CGGGACAAGG GTTACTGGGG ACGATGTTCT TATTGGAAAG 
ATAACGCCCA TACTGGACCC TGAAAGAAGT ACCAAAGAGG CGCCTGTTTA TGTGTACAAG 
GACAGCAGCA CTGCAATGCG TAGAACGGAA ACAGGAATTG TTGACACGGT TATTGTTACA 
AACAAGGATG GATACAAGTT TTCCAAGGTC AAGGTAAGGT CTGGGAGGAT TCCCCAGATG 
GGAGACAAGT TTGCGTCTAG ACATGCGCAG AAGGGAACAA TAGGAATTAC TTTGAGGCAG 
GAAGACATGC CCTTTACTTC TGAAGGGATA GTTCCGGACA TAATCATAAA CCCGCATGCG 
ATTCCCAGCC GTATGACCAT CGGGCATTTG ATTGAATGTC TCCTGGGCAA GGTTAGTGCA 
ATGAGTGGAG AGGAGGGGGA TGCAACACCA TTTAGTGGCG TGACTGTTGA CGGGATATCC 
TCCAGGTTGA AGTCGTATGG CTTCCAGCAA AGGGGGTTGG AGGTTATGTA CAATGGAATG 
ACAGGGAGAA AGCTCCGAGC TCAGATGTTC TTTGGGCCGA CATATTACCA ACGGCTTAAG 
CATATGGTTG ACGATAAGAT ACATGCTAGA GCTCGAGGCC CGCTCCAGAT ACTCACCAGG 
CAGCCTGTTG AGGGAAGGTC CAGGGATGGT GGGCTCCGGT TTGGAGAAAT GGAAAGAGAC 
TGTATAATTT CCCACGGGGC ATCTGCTTTT CTGAAAGAAA GGCTGATGGA TGTGAGCGAT 
GCATACTCTT GCTATGTGTG TGACTTTTGC GGGCTTCTTG CAATGGGTGG AAGCAAGGTG 
AATGAATGCA AGGGATGTAA CAACACCACG AATGTGAGCA TGGTTGAGAT TCCGTATGCC 
TTCAAACTCT TGATTCAGGA GCTGATGGGA ATGAATATCG CACCAAGGAT TCGCTTTGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.
Nature414(6862)450-3(2001 Nov)
Katinka MD,Duprat S,Cornillot E,Méténier G,Thomarat F,Prensier G,Barbe V,Peyretaillade E,Brottier P,Wincker P,Delbac F,El Alaoui H,Peyret P,Saurin W,Gouy M,Weissenbach J,Vivarès CP

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ECU10_0250 cDNA ORF clone, Encephalitozoon cuniculi GB-M1

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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