LOC6653158 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6653158
Entrez Gene ID	6653158
Full Name	uncharacterized protein DDB_G0283357
Synonyms	GK15791,GK15791-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_002075292.2	XP_002075328.2	uncharacterized protein
XM_023181322.1	XP_023037090.1	uncharacterized protein DDB_G0283357

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6653158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6653158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODf15239	XM_002075292.2 Latest version!	Drosophila willistoni uncharacterized protein (Dwil\GK15791), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODf28378	XM_023181322.1 Latest version!	Drosophila willistoni uncharacterized protein DDB_G0283357 (LOC6653158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6653158 ( XM_002075292.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002075328.2 Drosophila willistoni uncharacterized protein (Dwil\GK15791), partial mRNA.

Price & Availability↑

CloneID	ODf15239
Clone ID Related Accession (Same CDS sequence)	XM_002075292.2
Accession Version	XM_002075292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032912). On Dec 21, 2015 this sequence version replaced XM_002075292.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGATGATGA TGACGACAGC AACAACTTCA GCATCCGCAT CTCAAATGGC ATCGGGCCAC 
TTGACCGACT GCAGCAGCAT GCAGGGCTCG AGCACAAGCT CGGCATCCAG CGACGTGATG 
ACCCTGACCA CAACAGCGGC CTCCTCTTGG TGCGCCATTC CAGCCAGCTC TCGTCTGCGG 
GTGTTGCGTC TGGTGCGTCC GCATCAACGG CGATTGCTGG TGGTCCCGGA ACATAATGCC 
ACCTTTGGCT TTGCCGTACG CGGTGGAAAG GAGCACGGCA CTGGATTCTT TGTCTCCCAT 
GTGGAGCATG GAGGCGAAGC TCAATTGAAA GGTCTAAGGA TTGGCGATCA AATTCTGCGC 
ATCAATGGCT TTCGACTTGA TGATGCCGTC CATAAGGAGT TTATTCAATT GGTAGCCAGT 
CAGGACCGCG TCACATTAAA AGTGCGAGGT GTGGGAATGC TGCCAGTCAA GGACCAACCA 
GAGGAGCGTC TATCGTGGAG TGTGGTCAAG TTGGGCAGCG TCAGTGGAAC GCCCTCGGAA 
AGCTCCTTCA AAACGGAACG ACGCACAGCC AGCCGAGACA TCAGTGTAGT CCTTCATGTG 
GCTCCGCGAA CCAAACTTGG CCTGGGCATT TGCAAGGGTC CCGAATGGAA GCCGGGCATC 
TTTGTCCAGT TCACCAAAGA GCGAAGTGTT GCCCGAGAGG CTGGCCTCAG GCCAGGCGAT 
CAGATCCTCA GCGTGAACAG CATAGATTTT TCCGACGTGC TCTTTAGCGA GGCGGTGGCC 
GTAATGAAGA GTTCAAGCAA ATTGGATATG GTCGTACGGA CAGCGGCTGG CTGTGATCTA 
TTTCCAGGCG AGTCGAGTGG CTACAACAGT TCGGCCAGCT CAGTTACTGG TGATCAGAGT 
CCATGCTGGG CGGATGCCAA ATCGAAGCGT CTAACAGCCG TCCACGAGGA ACCCACACAC 
ACTGGCTCTT CTCGGCACTG GGCGGCGTCC AATAACAACA ACAACAACAG CAACAACAAG 
GGCGTCGAGG TGCACAAGCA AATGAACAAG ACGATTATCA AATTGTCGGA GAATGGAACG 
TCCATCAACA ACACCTACAT AGCCAGCTCC AAAGCGAACT CAATAGTCGA ACAATCGGCC 
CCATCGTCAA GAAGTGGTGG CCCAACGAAA TGCCATCAGC AGAAGCTGGG GGCTCCTCCG 
CCAGCACCAC CTCCAGCAAT CAACAGCAAC AGCAACAGCA ACGCGGCTAG TATTGCTCAA 
GTGTCGCCCA AGCAGAGAAC GACAATTGCT GGCGTCGCTA CCAACAATTC CACTGGCAGC 
GAACGCATCA GCAATGGACC AATTGGTGGC GGTGGCGGAG GCGGAGGCGG AGGTGGGGAT 
CAGCATAGCG CTCTGATGGA TGAATTCAAG CGAGCACACC AGCGTATGTT CAGGAACGGG 
TTCCATGAGA GCGAGCACAA GGTGAGCAGC CAGCAATTGT TGCATCTGAA GTTGCAACAC 
TTCAACAGCA ACGGCAGCAA CAACAACAAC AAAAACAACT TGTCCAATGG GCATGAGATG 
CCCACCATGC GACCAGTTAC AATTGGTCGT CCATTGGTGA CCATGTCCAC ATATGAGGAG 
CGTGGTGAGA CGCTAAAGCG TACCTCAATT ATGCCTGATG ATACCTGCTA TCGGAATAGC 
ACCAACAATG GCAACACAAA CAATAACAAC GCCAGCAATA ATCGCATATC GCTGATGAAT 
GGTGGCAACA GCAACAACAA CAACACCAAT GCAATTGAAA TTGGAAATGG TATCAATGGA 
AGCAATATCA ATGGAACGCC AGAGCTGCCA CCACCGCCGC CACAATTTGC CAATCCCCAG 
CAGTCATTAT ATAAGCAATT ACCCACACAA CAGCAGCAAC AGAAACAGCT GCAACTAAAA 
CTAAATGGTA ACATCGCCGC CGCCTCCAAC ACTCCTACAA GTGGCCAGCC TACAAAAATT 
AAACAGCCAT CCTCGCCCAT GCGTAGTGCT AGCATAAATG TCGGTGGTTT TCGACCTCCA 
GCAACGCCTC AGCCCGACTA CGATGCGGTG CAACTGCGTG GCAACACAAA TCAGCATCAA 
CTGCAGCAGC AGCAGCAACA ACAGCAGCAA CAACAACGTC GCACCCTTCG ACTGGGCACA 
GTTACCATTG GAGAATATGG AGATCAGCGT GCCAAGCGCA ACACTCTTCG TAAAATGAAC 
TCCATCAATG GGAGTAGTTC AAATGCCACC ACGCCCACAA GTCCATCTGC CTCAGCCTCA 
TCGAATGGCA TATTAAAGAA TGGGAATCGA AGCAGTGCCA GCTTTAGCAG CACTCGTCAA 
CCGCATACAG AAAAGTCCAT CAAGTTTGGC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002075328.2
CDS	1..2376
Translation	MMMMTTATTSASASQMASGHLTDCSSMQGSSTSSASSDVMTLTTTAASSWCAIPASSRLRVLRLVRPHQRRLLVVPEHNATFGFAVRGGKEHGTGFFVSHVEHGGEAQLKGLRIGDQILRINGFRLDDAVHKEFIQLVASQDRVTLKVRGVGMLPVKDQPEERLSWSVVKLGSVSGTPSESSFKTERRTASRDISVVLHVAPRTKLGLGICKGPEWKPGIFVQFTKERSVAREAGLRPGDQILSVNSIDFSDVLFSEAVAVMKSSSKLDMVVRTAAGCDLFPGESSGYNSSASSVTGDQSPCWADAKSKRLTAVHEEPTHTGSSRHWAASNNNNNNSNNKGVEVHKQMNKTIIKLSENGTSINNTYIASSKANSIVEQSAPSSRSGGPTKCHQQKLGAPPPAPPPAINSNSNSNAASIAQVSPKQRTTIAGVATNNSTGSERISNGPIGGGGGGGGGGGDQHSALMDEFKRAHQRMFRNGFHESEHKVSSQQLLHLKLQHFNSNGSNNNNKNNLSNGHEMPTMRPVTIGRPLVTMSTYEERGETLKRTSIMPDDTCYRNSTNNGNTNNNNASNNRISLMNGGNSNNNNTNAIEIGNGINGSNINGTPELPPPPPQFANPQQSLYKQLPTQQQQQKQLQLKLNGNIAAASNTPTSGQPTKIKQPSSPMRSASINVGGFRPPATPQPDYDAVQLRGNTNQHQLQQQQQQQQQQQRRTLRLGTVTIGEYGDQRAKRNTLRKMNSINGSSSNATTPTSPSASASSNGILKNGNRSSASFSSTRQPHTEKSIKFGN

Target ORF information:

RefSeq Version	XM_002075292.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK15791), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002075292.2

ORF Insert Sequence:

ATGATGATGA TGACGACAGC AACAACTTCA GCATCCGCAT CTCAAATGGC ATCGGGCCAC 
TTGACCGACT GCAGCAGCAT GCAGGGCTCG AGCACAAGCT CGGCATCCAG CGACGTGATG 
ACCCTGACCA CAACAGCGGC CTCCTCTTGG TGCGCCATTC CAGCCAGCTC TCGTCTGCGG 
GTGTTGCGTC TGGTGCGTCC GCATCAACGG CGATTGCTGG TGGTCCCGGA ACATAATGCC 
ACCTTTGGCT TTGCCGTACG CGGTGGAAAG GAGCACGGCA CTGGATTCTT TGTCTCCCAT 
GTGGAGCATG GAGGCGAAGC TCAATTGAAA GGTCTAAGGA TTGGCGATCA AATTCTGCGC 
ATCAATGGCT TTCGACTTGA TGATGCCGTC CATAAGGAGT TTATTCAATT GGTAGCCAGT 
CAGGACCGCG TCACATTAAA AGTGCGAGGT GTGGGAATGC TGCCAGTCAA GGACCAACCA 
GAGGAGCGTC TATCGTGGAG TGTGGTCAAG TTGGGCAGCG TCAGTGGAAC GCCCTCGGAA 
AGCTCCTTCA AAACGGAACG ACGCACAGCC AGCCGAGACA TCAGTGTAGT CCTTCATGTG 
GCTCCGCGAA CCAAACTTGG CCTGGGCATT TGCAAGGGTC CCGAATGGAA GCCGGGCATC 
TTTGTCCAGT TCACCAAAGA GCGAAGTGTT GCCCGAGAGG CTGGCCTCAG GCCAGGCGAT 
CAGATCCTCA GCGTGAACAG CATAGATTTT TCCGACGTGC TCTTTAGCGA GGCGGTGGCC 
GTAATGAAGA GTTCAAGCAA ATTGGATATG GTCGTACGGA CAGCGGCTGG CTGTGATCTA 
TTTCCAGGCG AGTCGAGTGG CTACAACAGT TCGGCCAGCT CAGTTACTGG TGATCAGAGT 
CCATGCTGGG CGGATGCCAA ATCGAAGCGT CTAACAGCCG TCCACGAGGA ACCCACACAC 
ACTGGCTCTT CTCGGCACTG GGCGGCGTCC AATAACAACA ACAACAACAG CAACAACAAG 
GGCGTCGAGG TGCACAAGCA AATGAACAAG ACGATTATCA AATTGTCGGA GAATGGAACG 
TCCATCAACA ACACCTACAT AGCCAGCTCC AAAGCGAACT CAATAGTCGA ACAATCGGCC 
CCATCGTCAA GAAGTGGTGG CCCAACGAAA TGCCATCAGC AGAAGCTGGG GGCTCCTCCG 
CCAGCACCAC CTCCAGCAAT CAACAGCAAC AGCAACAGCA ACGCGGCTAG TATTGCTCAA 
GTGTCGCCCA AGCAGAGAAC GACAATTGCT GGCGTCGCTA CCAACAATTC CACTGGCAGC 
GAACGCATCA GCAATGGACC AATTGGTGGC GGTGGCGGAG GCGGAGGCGG AGGTGGGGAT 
CAGCATAGCG CTCTGATGGA TGAATTCAAG CGAGCACACC AGCGTATGTT CAGGAACGGG 
TTCCATGAGA GCGAGCACAA GGTGAGCAGC CAGCAATTGT TGCATCTGAA GTTGCAACAC 
TTCAACAGCA ACGGCAGCAA CAACAACAAC AAAAACAACT TGTCCAATGG GCATGAGATG 
CCCACCATGC GACCAGTTAC AATTGGTCGT CCATTGGTGA CCATGTCCAC ATATGAGGAG 
CGTGGTGAGA CGCTAAAGCG TACCTCAATT ATGCCTGATG ATACCTGCTA TCGGAATAGC 
ACCAACAATG GCAACACAAA CAATAACAAC GCCAGCAATA ATCGCATATC GCTGATGAAT 
GGTGGCAACA GCAACAACAA CAACACCAAT GCAATTGAAA TTGGAAATGG TATCAATGGA 
AGCAATATCA ATGGAACGCC AGAGCTGCCA CCACCGCCGC CACAATTTGC CAATCCCCAG 
CAGTCATTAT ATAAGCAATT ACCCACACAA CAGCAGCAAC AGAAACAGCT GCAACTAAAA 
CTAAATGGTA ACATCGCCGC CGCCTCCAAC ACTCCTACAA GTGGCCAGCC TACAAAAATT 
AAACAGCCAT CCTCGCCCAT GCGTAGTGCT AGCATAAATG TCGGTGGTTT TCGACCTCCA 
GCAACGCCTC AGCCCGACTA CGATGCGGTG CAACTGCGTG GCAACACAAA TCAGCATCAA 
CTGCAGCAGC AGCAGCAACA ACAGCAGCAA CAACAACGTC GCACCCTTCG ACTGGGCACA 
GTTACCATTG GAGAATATGG AGATCAGCGT GCCAAGCGCA ACACTCTTCG TAAAATGAAC 
TCCATCAATG GGAGTAGTTC AAATGCCACC ACGCCCACAA GTCCATCTGC CTCAGCCTCA 
TCGAATGGCA TATTAAAGAA TGGGAATCGA AGCAGTGCCA GCTTTAGCAG CACTCGTCAA 
CCGCATACAG AAAAGTCCAT CAAGTTTGGC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6653158 ( XM_023181322.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023037090.1 Drosophila willistoni uncharacterized protein DDB_G0283357 (LOC6653158), mRNA.

Price & Availability↑

CloneID	ODf28378
Clone ID Related Accession (Same CDS sequence)	XM_023181322.1
Accession Version	XM_023181322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	uncharacterized protein DDB_G0283357
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAC GACAGCAACA ACTTCAGCAT CCGCATCTCA AATGGCATCG 
GGCCACTTGA CCGACTGCAG CAGCATGCAG GGCTCGAGCA CAAGCTCGGC ATCCAGCGAC 
GTGATGACCC TGACCACAAC AGCGGCCTCC TCTTGGTGCG CCATTCCAGC CAGCTCTCGT 
CTGCGGGTGT TGCGTCTGGT GCGTCCGCAT CAACGGCGAT TGCTGGTGGT CCCGGAACAT 
AATGCCACCT TTGGCTTTGC CGTACGCGGT GGAAAGGAGC ACGGCACTGG ATTCTTTGTC 
TCCCATGTGG AGCATGGAGG CGAAGCTCAA TTGAAAGGTC TAAGGATTGG CGATCAAATT 
CTGCGCATCA ATGGCTTTCG ACTTGATGAT GCCGTCCATA AGGAGTTTAT TCAATTGGTA 
GCCAGTCAGG ACCGCGTCAC ATTAAAAGTG CGAGGTGTGG GAATGCTGCC AGTCAAGGAC 
CAACCAGAGG AGCGTCTATC GTGGAGTGTG GTCAAGTTGG GCAGCGTCAG TGGAACGCCC 
TCGGAAAGCT CCTTCAAAAC GGAACGACGC ACAGCCAGCC GAGACATCAG TGTAGTCCTT 
CATGTGGCTC CGCGAACCAA ACTTGGCCTG GGCATTTGCA AGGGTCCCGA ATGGAAGCCG 
GGCATCTTTG TCCAGTTCAC CAAAGAGCGA AGTGTTGCCC GAGAGGCTGG CCTCAGGCCA 
GGCGATCAGA TCCTCAGCGT GAACAGCATA GATTTTTCCG ACGTGCTCTT TAGCGAGGCG 
GTGGCCGTAA TGAAGAGTTC AAGCAAATTG GATATGGTCG TACGGACAGC GGCTGGCTGT 
GATCTATTTC CAGGCGAGTC GAGTGGCTAC AACAGTTCGG CCAGCTCAGT TACTGGTGAT 
CAGAGTCCAT GCTGGGCGGA TGCCAAATCG AAGCGTCTAA CAGCCGTCCA CGAGGAACCC 
ACACACACTG GCTCTTCTCG GCACTGGGCG GCGTCCAATA ACAACAACAA CAACAGCAAC 
AACAAGGGCG TCGAGGTGCA CAAGCAAATG AACAAGACGA TTATCAAATT GTCGGAGAAT 
GGAACGTCCA TCAACAACAC CTACATAGCC AGCTCCAAAG CGAACTCAAT AGTCGAACAA 
TCGGCCCCAT CGTCAAGAAG TGGTGGCCCA ACGAAATGCC ATCAGCAGAA GCTGGGGGCT 
CCTCCGCCAG CACCACCTCC AGCAATGTAT AAAACCGAGG CCGAGTCATC TTCAATGGGG 
GGTGGTGGTG ATGGTGGTGC TGGTGGTGGT GGTGTCGGAA GCAGCAGCAG CCTTTCTAGT 
GCCATAAACG AAGAGCTCAA GAAACGCAAA GAGAAGCAAC AATATCAGCA ACAACATCTG 
CGCAATCGAG ATGAAGATCG TCAGCAACAG CAGAGACCGC AACCGCACAA TGGTAACCAC 
CGCAGCAACG GCAACAGCAG CAGCAACAGC AACAGCAACA GCAACGCGGC TAGTATTGCT 
CAAGTGTCGC CCAAGCAGAG AACGACAATT GCTGGCGTCG CTACCAACAA TTCCACTGGC 
AGCGAACGCA TCAGCAATGG ACCAATTGGT GGCGGTGGCG GAGGCGGAGG CGGAGGTGGG 
GATCAGCATA GCGCTCTGAT GGATGAATTC AAGCGAGCAC ACCAGCGTAT GTTCAGGAAC 
GGGTTCCATG AGAGCGAGCA CAAGGAGCGT GGTGAGACGC TAAAGCGTAC CTCAATTATG 
CCTGATGATA CCTGCTATCG GAATAGCACC AACAATGGCA ACACAAACAA TAACAACGCC 
AGCAATAATC GCATATCGCT GATGAATGGT GGCAACAGCA ACAACAACAA CACCAATGCA 
ATTGAAATTG GAAATGGTAT CAATGGAAGC AATATCAATG GAACGCCAGA GCTGCCACCA 
CCGCCGCCAC AATTTGCCAA TCCCCAGCAG TCATTATATA AGCAATTACC CACACAACAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAACAA CAGCAACAGA AACAGCTGCA ACTAAAACTA 
AATGGTAACA TCGCCGCCGC CTCCAACACT CCTACAAGTG GCCAGCCTAC AAAAATTAAA 
CAGCCATCCT CGCCCATGCG TAGTGCTAGC ATAAATGTCG GTGGTTTTCG ACCTCCAGCA 
ACGCCTCAGC CCGACTACGA TGCGGTGCAA CTGCGTGGCA ACACAAATCA GCATCAACTG 
CAGCAGCAGC AGCAACAACA GCAGCAACAA CAACGTCGCA CCCTTCGACT GGGCACAGTT 
ACCATTGGAG AATATGGAGA TCAGCGTGCC AAGCGCAACA CTCTTCGTAA AATGAACTCC 
ATCAATGGGA GTAGTTCAAA TGCCACCACG CCCACAAGTC CATCTGCCTC AGCCTCATCG 
AATGGCATAT TAAAGAATGG GAATCGAAGC AGTGCCAGCT TTAGCAGCAC TCGTCAACCG 
CATACAGAAA AGTCCATCAA GTTTGGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023037090.1
CDS	143..2635
Translation	MMMMMMTTATTSASASQMASGHLTDCSSMQGSSTSSASSDVMTLTTTAASSWCAIPASSRLRVLRLVRPHQRRLLVVPEHNATFGFAVRGGKEHGTGFFVSHVEHGGEAQLKGLRIGDQILRINGFRLDDAVHKEFIQLVASQDRVTLKVRGVGMLPVKDQPEERLSWSVVKLGSVSGTPSESSFKTERRTASRDISVVLHVAPRTKLGLGICKGPEWKPGIFVQFTKERSVAREAGLRPGDQILSVNSIDFSDVLFSEAVAVMKSSSKLDMVVRTAAGCDLFPGESSGYNSSASSVTGDQSPCWADAKSKRLTAVHEEPTHTGSSRHWAASNNNNNNSNNKGVEVHKQMNKTIIKLSENGTSINNTYIASSKANSIVEQSAPSSRSGGPTKCHQQKLGAPPPAPPPAMYKTEAESSSMGGGGDGGAGGGGVGSSSSLSSAINEELKKRKEKQQYQQQHLRNRDEDRQQQQRPQPHNGNHRSNGNSSSNSNSNSNAASIAQVSPKQRTTIAGVATNNSTGSERISNGPIGGGGGGGGGGGDQHSALMDEFKRAHQRMFRNGFHESEHKERGETLKRTSIMPDDTCYRNSTNNGNTNNNNASNNRISLMNGGNSNNNNTNAIEIGNGINGSNINGTPELPPPPPQFANPQQSLYKQLPTQQQQQQQQQQQQQQQKQLQLKLNGNIAAASNTPTSGQPTKIKQPSSPMRSASINVGGFRPPATPQPDYDAVQLRGNTNQHQLQQQQQQQQQQQRRTLRLGTVTIGEYGDQRAKRNTLRKMNSINGSSSNATTPTSPSASASSNGILKNGNRSSASFSSTRQPHTEKSIKFGN

Target ORF information:

RefSeq Version	XM_023181322.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein DDB_G0283357 (LOC6653158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023181322.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAC GACAGCAACA ACTTCAGCAT CCGCATCTCA AATGGCATCG 
GGCCACTTGA CCGACTGCAG CAGCATGCAG GGCTCGAGCA CAAGCTCGGC ATCCAGCGAC 
GTGATGACCC TGACCACAAC AGCGGCCTCC TCTTGGTGCG CCATTCCAGC CAGCTCTCGT 
CTGCGGGTGT TGCGTCTGGT GCGTCCGCAT CAACGGCGAT TGCTGGTGGT CCCGGAACAT 
AATGCCACCT TTGGCTTTGC CGTACGCGGT GGAAAGGAGC ACGGCACTGG ATTCTTTGTC 
TCCCATGTGG AGCATGGAGG CGAAGCTCAA TTGAAAGGTC TAAGGATTGG CGATCAAATT 
CTGCGCATCA ATGGCTTTCG ACTTGATGAT GCCGTCCATA AGGAGTTTAT TCAATTGGTA 
GCCAGTCAGG ACCGCGTCAC ATTAAAAGTG CGAGGTGTGG GAATGCTGCC AGTCAAGGAC 
CAACCAGAGG AGCGTCTATC GTGGAGTGTG GTCAAGTTGG GCAGCGTCAG TGGAACGCCC 
TCGGAAAGCT CCTTCAAAAC GGAACGACGC ACAGCCAGCC GAGACATCAG TGTAGTCCTT 
CATGTGGCTC CGCGAACCAA ACTTGGCCTG GGCATTTGCA AGGGTCCCGA ATGGAAGCCG 
GGCATCTTTG TCCAGTTCAC CAAAGAGCGA AGTGTTGCCC GAGAGGCTGG CCTCAGGCCA 
GGCGATCAGA TCCTCAGCGT GAACAGCATA GATTTTTCCG ACGTGCTCTT TAGCGAGGCG 
GTGGCCGTAA TGAAGAGTTC AAGCAAATTG GATATGGTCG TACGGACAGC GGCTGGCTGT 
GATCTATTTC CAGGCGAGTC GAGTGGCTAC AACAGTTCGG CCAGCTCAGT TACTGGTGAT 
CAGAGTCCAT GCTGGGCGGA TGCCAAATCG AAGCGTCTAA CAGCCGTCCA CGAGGAACCC 
ACACACACTG GCTCTTCTCG GCACTGGGCG GCGTCCAATA ACAACAACAA CAACAGCAAC 
AACAAGGGCG TCGAGGTGCA CAAGCAAATG AACAAGACGA TTATCAAATT GTCGGAGAAT 
GGAACGTCCA TCAACAACAC CTACATAGCC AGCTCCAAAG CGAACTCAAT AGTCGAACAA 
TCGGCCCCAT CGTCAAGAAG TGGTGGCCCA ACGAAATGCC ATCAGCAGAA GCTGGGGGCT 
CCTCCGCCAG CACCACCTCC AGCAATGTAT AAAACCGAGG CCGAGTCATC TTCAATGGGG 
GGTGGTGGTG ATGGTGGTGC TGGTGGTGGT GGTGTCGGAA GCAGCAGCAG CCTTTCTAGT 
GCCATAAACG AAGAGCTCAA GAAACGCAAA GAGAAGCAAC AATATCAGCA ACAACATCTG 
CGCAATCGAG ATGAAGATCG TCAGCAACAG CAGAGACCGC AACCGCACAA TGGTAACCAC 
CGCAGCAACG GCAACAGCAG CAGCAACAGC AACAGCAACA GCAACGCGGC TAGTATTGCT 
CAAGTGTCGC CCAAGCAGAG AACGACAATT GCTGGCGTCG CTACCAACAA TTCCACTGGC 
AGCGAACGCA TCAGCAATGG ACCAATTGGT GGCGGTGGCG GAGGCGGAGG CGGAGGTGGG 
GATCAGCATA GCGCTCTGAT GGATGAATTC AAGCGAGCAC ACCAGCGTAT GTTCAGGAAC 
GGGTTCCATG AGAGCGAGCA CAAGGAGCGT GGTGAGACGC TAAAGCGTAC CTCAATTATG 
CCTGATGATA CCTGCTATCG GAATAGCACC AACAATGGCA ACACAAACAA TAACAACGCC 
AGCAATAATC GCATATCGCT GATGAATGGT GGCAACAGCA ACAACAACAA CACCAATGCA 
ATTGAAATTG GAAATGGTAT CAATGGAAGC AATATCAATG GAACGCCAGA GCTGCCACCA 
CCGCCGCCAC AATTTGCCAA TCCCCAGCAG TCATTATATA AGCAATTACC CACACAACAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAACAA CAGCAACAGA AACAGCTGCA ACTAAAACTA 
AATGGTAACA TCGCCGCCGC CTCCAACACT CCTACAAGTG GCCAGCCTAC AAAAATTAAA 
CAGCCATCCT CGCCCATGCG TAGTGCTAGC ATAAATGTCG GTGGTTTTCG ACCTCCAGCA 
ACGCCTCAGC CCGACTACGA TGCGGTGCAA CTGCGTGGCA ACACAAATCA GCATCAACTG 
CAGCAGCAGC AGCAACAACA GCAGCAACAA CAACGTCGCA CCCTTCGACT GGGCACAGTT 
ACCATTGGAG AATATGGAGA TCAGCGTGCC AAGCGCAACA CTCTTCGTAA AATGAACTCC 
ATCAATGGGA GTAGTTCAAA TGCCACCACG CCCACAAGTC CATCTGCCTC AGCCTCATCG 
AATGGCATAT TAAAGAATGG GAATCGAAGC AGTGCCAGCT TTAGCAGCAC TCGTCAACCG 
CATACAGAAA AGTCCATCAA GTTTGGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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LOC6653158 cDNA ORF clone, Drosophila willistoni

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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