DWIL\GK15712 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DWIL\GK15712
Entrez Gene ID	6653150
Full Name	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
Synonyms	GK15712,GK15712-PA,GK15712-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_015176453.1	XP_015031939.1	uncharacterized protein, isoform B
XM_002075301.3	XP_002075337.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform X1
XM_015176453.2	XP_015031939.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform X2
XM_002075301.2	XP_002075337.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DWIL\GK15712 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWIL\GK15712 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODf14662	XM_002075301.3 Latest version!	Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODf15333	XM_015176453.2 Latest version!	Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODf15333	XM_015176453.1	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK15712), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODf14662	XM_002075301.2	Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK15712), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWIL\GK15712 ( XM_002075301.3 ) cDNA ORF clone, Drosophila willistoni -> XP_002075337.1 Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODf14662
Clone ID Related Accession (Same CDS sequence)	XM_002075301.3 , XM_002075301.2
Accession Version	XM_002075301.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 4, 2017 this sequence version replaced XM_002075301.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGAGT TGGCAAAAGA ATTTGCGTCT CATCACTCAT 
AACAATAAAG CCACCAATGT CTGTCCACGT CTGAATCTAA TGAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002075337.1
CDS	540..3827
Translation	MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAGGTPRDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKSWQKNLRLITHNNKATNVCPRLNLMKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNATMTKEQFTFEKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILFPLYEEKRCTEIINDYELDEEKKKNVQLSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHVLEPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAAPEAAAADAAAEGGGGAEAEAAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQTSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVPNNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKMIGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIRNDANIKNAINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDLKDLNTKQAKTSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHAKQVEEFSTDVQKLMDMYKIEEEAYKTQGKTEFYA

Target ORF information:

RefSeq Version	XM_002075301.3
Organism	Drosophila willistoni
Definition	Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002075301.3

ORF Insert Sequence:

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGAGT TGGCAAAAGA ATTTGCGTCT CATCACTCAT 
AACAATAAAG CCACCAATGT CTGTCCACGT CTGAATCTAA TGAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK15712 ( XM_002075301.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002075337.1 Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK15712), mRNA.

Price & Availability↑

CloneID	ODf14662
Clone ID Related Accession (Same CDS sequence)	XM_002075301.3 , XM_002075301.2
Accession Version	XM_002075301.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform A
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032912). On Dec 21, 2015 this sequence version replaced XM_002075301.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGAGT TGGCAAAAGA ATTTGCGTCT CATCACTCAT 
AACAATAAAG CCACCAATGT CTGTCCACGT CTGAATCTAA TGAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002075337.1
CDS	215..3502
Translation	MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAGGTPRDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKSWQKNLRLITHNNKATNVCPRLNLMKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNATMTKEQFTFEKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILFPLYEEKRCTEIINDYELDEEKKKNVQLSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHVLEPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAAPEAAAADAAAEGGGGAEAEAAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQTSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVPNNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKMIGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIRNDANIKNAINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDLKDLNTKQAKTSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHAKQVEEFSTDVQKLMDMYKIEEEAYKTQGKTEFYA

Target ORF information:

RefSeq Version	XM_002075301.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK15712), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002075301.2

ORF Insert Sequence:

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGAGT TGGCAAAAGA ATTTGCGTCT CATCACTCAT 
AACAATAAAG CCACCAATGT CTGTCCACGT CTGAATCTAA TGAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK15712 ( XM_015176453.1 ) cDNA ORF clone, Drosophila willistoni -> XP_015031939.1 Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK15712), mRNA.

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CloneID	ODf15333
Clone ID Related Accession (Same CDS sequence)	XM_015176453.1 , XM_015176453.2
Accession Version	XM_015176453.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform B
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032912). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGATC TGGCTAGATG GCATACGAAA AATAACCCAT 
AATGTCAAAG CGAATAATAT CTGCCCTATG ATGTGTCTAA GAAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015031939.1
CDS	215..3502
Translation	MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAGGTPRDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKIWLDGIRKITHNVKANNICPMMCLRKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNATMTKEQFTFEKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILFPLYEEKRCTEIINDYELDEEKKKNVQLSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHVLEPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAAPEAAAADAAAEGGGGAEAEAAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQTSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVPNNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKMIGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIRNDANIKNAINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDLKDLNTKQAKTSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHAKQVEEFSTDVQKLMDMYKIEEEAYKTQGKTEFYA

Target ORF information:

RefSeq Version	XM_015176453.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK15712), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015176453.1

ORF Insert Sequence:

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGATC TGGCTAGATG GCATACGAAA AATAACCCAT 
AATGTCAAAG CGAATAATAT CTGCCCTATG ATGTGTCTAA GAAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK15712 ( XM_015176453.2 ) cDNA ORF clone, Drosophila willistoni -> XP_015031939.1 Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X2, mRNA.

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CloneID	ODf15333
Clone ID Related Accession (Same CDS sequence)	XM_015176453.1 , XM_015176453.2
Accession Version	XM_015176453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 4, 2017 this sequence version replaced XM_015176453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGATC TGGCTAGATG GCATACGAAA AATAACCCAT 
AATGTCAAAG CGAATAATAT CTGCCCTATG ATGTGTCTAA GAAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015031939.1
CDS	394..3681
Translation	MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAGGTPRDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKIWLDGIRKITHNVKANNICPMMCLRKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNATMTKEQFTFEKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILFPLYEEKRCTEIINDYELDEEKKKNVQLSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHVLEPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAAPEAAAADAAAEGGGGAEAEAAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQTSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVPNNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKMIGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIRNDANIKNAINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDLKDLNTKQAKTSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHAKQVEEFSTDVQKLMDMYKIEEEAYKTQGKTEFYA

Target ORF information:

RefSeq Version	XM_015176453.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (LOC6653150), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015176453.2

ORF Insert Sequence:

ATGACAAAGA AATACGAATT CGATTGGATC ATTCCGGTGC CACCGGAATT GACCACTGGC 
TGTGTGTTCG ATCGCTGGTT CGAGAATGAG AAGGAAACCA AAGAGAATGA CTTTGAACGT 
GATGCCCTCT TCAAGGTGGA TGAATATGGA TTCTTTCTTT ATTGGAAGAG TGAGGGAAGG 
GATGGCGATG TCATTGAGCT GTGTCAAGTG AGCGACATTC GTGCTGGCGG CACACCAAGG 
GATCCCAAAA TACTGGATAA GGTTACAAAA AAGAATGGCA CGAATATACC AGAATTGGAT 
AAACGTTCAT TGACCATATG CTCGAATACG GATTATATCA ATATAACATA TCATCATGTT 
ATTTGTCCAG ATGCGGCTAC GGCTAAGATC TGGCTAGATG GCATACGAAA AATAACCCAT 
AATGTCAAAG CGAATAATAT CTGCCCTATG ATGTGTCTAA GAAAACATTG GATGCGTTTA 
AGCTATTGCG TGGAGAAGAG TGGTAAAATT CCAGTTAAAA CTTTGGCTAA AACATTTGCC 
TCGGGCAAGA CGGAAAAGCT GGTCTATACA TGCATCAAAG ATGCCGGTTT GCCAGATGAT 
AAGAATGCCA CCATGACAAA GGAGCAATTC ACATTCGAGA AATTCTATGC TTTATATCAC 
AAAGTCTGTC CACGTAATGA CATCGAGGAG CTATTCACAT CCATAACCAA GGGCAAACAG 
GATTTCATTA GTCTAGAGCA ATTTATTCAA TTCATGAATG ACAAGCAACG TGATCCCAGA 
ATGAATGAAA TTCTATTTCC ACTCTATGAG GAGAAACGAT GCACGGAGAT TATCAACGAT 
TATGAACTAG ATGAGGAGAA GAAGAAGAAT GTTCAACTCT CTTTGGATGG CTTTAAGCGA 
TATCTAATGT CCGATGAGAA CGCTCCCGTG TTTCTGGATC GCCTGGACTT CTATATGGAA 
ATGGATCAAC CATTGGCCCA TTACTATATA AATAGTTCGC ATAATACTTA TCTATCGGGT 
CGCCAAATCG GAGGTAAAAG CTCGGTGGAA ATGTATCGCC AGACCCTGTT GGCCGGCTGC 
CGTTGTGTGG AGCTTGATTG TTGGAATGGC AAGGGCGAGG ATGAGGAGCC AATTGTTACT 
CACGGCCATG CCTACTGTAC AGAAATTCTC TTCAAGGACT GCATTCAAGC CATAGCGGAT 
TGCGCTTTTG TTTCATCGGA ATATCCCGTG ATTTTGTCCT TTGAGAATCA TTGCAATCGT 
GCCCAGCAAT ATAAACTAGC TAAATACTGT GATGACTTCT TTGGTGATCT ATTGCTGAAG 
GAACCATTGC CAGATCATGT GCTGGAGCCG GGCTTGCCCC TGCCACCGCC GTGCAAGCTG 
AAGCGCAAGA TTCTCATTAA GAACAAGCGC ATGAAGCCCG AAGTGGAGAA GGTCGAACTG 
GAGCTATGGC TTAAGGGTGA ATTGAAGACC GATGACGATC CGGAAGAGGA CGCCAGTGCC 
GGCAAGCCGC CAGAAGCAGC TGCTGCCCCA GCGCCAGCAG CACCCGAAGC CGCTGCTGCC 
GATGCTGCCG CTGAAGGTGG CGGCGGCGCC GAAGCGGAGG CAGCTGCAGC CAATTACAGT 
GGTTCCACCA CCAATGTACA TCCCTGGCTC TCATCGATGG TCAATTATGC TCAGCCCATT 
AAATTCCAGG GCTTCGACAA GGCAATTGAG AAGAACATTG CCCATAATAT GTCATCGTTT 
GCGGAATCGG CTGGCATGAA TTACCTGAAA CAGAGCTCAA TTGATTTTGT CAATTATAAT 
AAGCGTCAGA TGTCTCGCAT CTATCCGAAG GGCACGCGAG CCGATTCGTC CAACTATATG 
CCACAGGTAT TCTGGAATGC CGGCTGTCAG ATGGTCTCAC TGAATTTCCA AACCTCCGAT 
CTGCCCATGC AACTGAACCA GGGTAAATTT GAATATAATG GCGGCTGTGG CTACTTGCTA 
AAGCCGGACT TTATGCGGCG TGCCGATAAG GACTTCGATC CGTTTGCCGA TGCCCCCGTG 
GACGGTGTGA TTGCTGCTCA ATGTTCGGTT AAAGTGATTG CCGGTCAATT TTTGTCGGAC 
AAGAAGGTTG GCACCTATGT GGAGGTGGAT ATGTTTGGCT TGCCCTCCGA TACGGTTAAA 
AAGGAGTTTC GTACACGTCT GGTCCCCAAC AATGGACTGA ATCCTGTTTA CAACGAGGAT 
CCATTTGTTT TCCGCAAAGT TGTTCTACCA GATTTGGCAG TGCTGCGCTT CGGCGTCTAT 
GAGGAGAGCG GCAAGATGAT CGGCCAGCGT ATTCTACCGC TGGATGGTCT CCAGGCTGGC 
TATCGGCATG TCTCGCTGCG CACGGAGGCC AACTTCCCAA TGTCGCTGCC CATGCTGTTT 
GTGAACATCG AATTAAAGAT TTATGTACCT GATGGCTTTG AGGATTTCAT GGCCATGCTG 
TCAGATCCAC GCGGTTTTGC CGGTGCGGCC AAGCAGCAGA ACGAACAGAT GAAGGCTCTT 
GGAATTGAGG AGCAAAGCGG CGGTGCCGCT CGTGATGCCG GCAAGGCCAA GGAGGAGGAG 
AAAAAAGAGC CGCCATTGGT CTTTGAGCCA GTGACGCTGG AATCTTTGCG TCAAGAGAAG 
GGCTTCCAGA AGGTCGGCAA GAAGCAGATC AAGGAGCTCG ACACCCTACG CAAAAAGCAT 
GCCAAGGAGC GCACCTCAGT GCAAAAGACC CAAAATGCCG CCATCGATAA GCTAATTAAG 
GGCAAGAGCA AAGATGACAT ACGCAACGAT GCCAACATCA AGAATGCCAT CAATGATCAG 
ACCAAACAGT GGACCGACAT GATTGCACGC CACCGCAAAG AGGAATGGGA TATGCTGCGA 
CAGCATGTCC AGGACTCTCA GGATGCCATG AAGGCTCTTA TGCTAACAGT GCAAGCAGCC 
CAAATCAAGC AGCTAGAAGA TCGTCATGCC AGAGATCTAA AAGATTTAAA TACCAAGCAG 
GCCAAAACAT CTGCAGATAC CGCCAAGGAG GTGCAGAATG ACAAGACGCT AAAGACCAAA 
AATGAAAAGG ATCGTCGTTT GCGTGAGAAA CGTCAGAATA ATGTCAAGCG TTTCATGGAG 
GAGAAAAAGC AAATCGGCGT TAAACAGGGA CGCGCCATGG AGAAATTAAA ATTGGCTCAT 
GCCAAACAAG TGGAGGAGTT TAGTACCGAT GTGCAAAAGC TTATGGATAT GTACAAAATC 
GAGGAGGAGG CCTACAAGAC CCAAGGAAAA ACTGAGTTTT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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DWIL\GK15712 cDNA ORF clone, Drosophila willistoni

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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