DWIL\GK17168 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DWIL\GK17168
Entrez Gene ID	6652966
Full Name	protein kinase dsk1
Synonyms	GK17168,GK17168-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_023181357.1	XP_023037125.1	protein kinase dsk1
XM_002075243.2	XP_002075279.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DWIL\GK17168 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWIL\GK17168 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODf15228	XM_002075243.2 Latest version!	Drosophila willistoni uncharacterized protein (Dwil\GK17168), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODf28409	XM_023181357.1 Latest version!	Drosophila willistoni protein kinase dsk1 (LOC6652966), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWIL\GK17168 ( XM_002075243.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002075279.2 Drosophila willistoni uncharacterized protein (Dwil\GK17168), partial mRNA.

Price & Availability↑

CloneID	ODf15228
Clone ID Related Accession (Same CDS sequence)	XM_002075243.2
Accession Version	XM_002075243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032912). On Dec 21, 2015 this sequence version replaced XM_002075243.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAATGG AACATAATTT CGCAAGCAAT CGCAATGGAA TTGACGATGA GGTTGACGTT 
GAGGAATTCT GTAGCTTCAC TTCAAAGTCA TCTGCGGCAG AAGTAGACGA GGAACATAGT 
CAAAATACTA ATGGCGAGGA AAGTCCAAGC GAATATCGCG TGGGTGGCTA TCATCCTATT 
GCTATTGGGG ATATCTTTCA GAATCGTTAT TACACATTGC GAAAACTTGG TTGGGGACAT 
TTCTCTACAG TTTGGCTATG CTACGATGCG AGATGTGAAC GCTATTGCGC CATAAAGGTG 
GTAAAGTCGG CAGAGCATTT CACCGAAACG GGACGAGATG AGATACGTTT GTCCCGCACC 
ATATCCAATC GTAATTGGCA TCCATTGCGT CAACGGTTGA TCGAATTGAT AGATTTTTTC 
TATATAAGCG GTCCGAATGG TACCCATTTG TGTCTAGTCT TTGAGGCATT GGGCGAAAAT 
CTCCTGAGTT TGATTCAACG TTCCCGCTAT CAGGGCTTGC CACTGTGGAA TGTTAAGCAG 
ATTGCCAAAC AGGTATTAGA GGGCCTATGC TTTTTGCACA CCCAATGCTC CATAATACAC 
ACCGATTTGA AGCCCGAGAA CGTGCTCCTG ATGGTTGATG ATTACACATT TCGTACGCAG 
GCGAATGATG CAGCTGAAAC TAGTAATAGC AATAGAAGTA GACAGCATAA TAATGGCATA 
ACTGCTACAA TGATGCGTCC CTCCTGCCCG CACAATGAGG AGGGGGAGGA GGGAGGTGAT 
CATCGCAACA GCGATTCCAA ACTCACAAAA ACGGCTAAAA GACGTCTACG TTCGAAAATC 
AAGCGGAGTA TTAACTTTTT TCAATCTCAT CGGCTTATGT TGCGACAACG TGGCATTGAC 
GATCTACTTC ATCTGGCCAC CAGGGGCCTG TTGACCCCCA CTATGGCTGC CTCGTGTGTG 
CGTGGCAATT TATCATATTT GCCCTTCACA TTCGATAATC TGATCATATT AGATGACAAG 
GACATGGAAC AATTGAAGAA ATTGACCATG ATCGAACGAG TTGGTGATAT GCCATCGAAA 
GATGCAATCG AAGAGAAACC CAGTTCTTCG AGTCGTACAA CATTGTTGAA AAAACATCTG 
CTTAAATGCG AGAATTCTCG AAAAAATGAA AGCTTTAAAC ATTTGGAATC ATGCTCGAAA 
ATGTTAAAAC TTCTTATGAC CAGTCCGGAG AAATTTATGC GATATGTGAA AAAGCGTATA 
GAGGAAAAGA CCAAGTCGTC GGTGCATAAG CTCCCTTCTG CAATTCCTAG ACTGAAACAA 
TCTCCAAGGA ATGGACTAGC CGCCAACAGC ACCGCAACCT TCAGTGAATT CTTTCCATTG 
AGTGCACATA ATAAGGAGTT ACCCGAACCC GGTATCAATT TAATATCACG CAAGGATCCT 
GCCACAGAGC CGTGTAAATT GAGTGTTAAG ATCGCCGATA TTGGTAATGC CTGCTGGTTC 
CATCACCATT TCACCGATGA TATACAAACC CGGGAATATC GTGCCGTCGA AGTCATTTTG 
GGTGCCGGCT ATGATGAAAC GGCGGACGTA TGGAGTGCTG CATGTTTGTT TTGGGAAGTG 
GCAACCGGTG ATTATCTCTT TGATCCGCAC TTAACGCGTG AGGCAGACGC CTCACAGGAT 
GAGGCCCACA TAGCAAATAT CATCGAGACC TGCGGCCGTA TACCTGAAGA ACTAATTAGT 
TATGGCGACT ATGCATCTGC AATTTTTGAA GGTCGCGAAT TGCGAAACGT GAAAGATTTG 
CGTCCACGAT CCTTGACTAA TGTGCTAATC GACCGCTATC GTTGGCCCGA CAAGGATGCC 
GAAGAGTTTG TTGCATTTCT AATGCCCATG CTTCAAACAG ATCCCAGGCT TCGGGTTAGT 
GCTGCCAATG CTATGCACCA CAAGTGGTTA AAGCTGGAAA AGGATGATCG GGATGAGGTT 
ACTGATAAAA AGGAGTCCAA GTTGAGCTCC GGCCAAGAAG AAACTACTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002075279.2
CDS	1..2034
Translation	MSMEHNFASNRNGIDDEVDVEEFCSFTSKSSAAEVDEEHSQNTNGEESPSEYRVGGYHPIAIGDIFQNRYYTLRKLGWGHFSTVWLCYDARCERYCAIKVVKSAEHFTETGRDEIRLSRTISNRNWHPLRQRLIELIDFFYISGPNGTHLCLVFEALGENLLSLIQRSRYQGLPLWNVKQIAKQVLEGLCFLHTQCSIIHTDLKPENVLLMVDDYTFRTQANDAAETSNSNRSRQHNNGITATMMRPSCPHNEEGEEGGDHRNSDSKLTKTAKRRLRSKIKRSINFFQSHRLMLRQRGIDDLLHLATRGLLTPTMAASCVRGNLSYLPFTFDNLIILDDKDMEQLKKLTMIERVGDMPSKDAIEEKPSSSSRTTLLKKHLLKCENSRKNESFKHLESCSKMLKLLMTSPEKFMRYVKKRIEEKTKSSVHKLPSAIPRLKQSPRNGLAANSTATFSEFFPLSAHNKELPEPGINLISRKDPATEPCKLSVKIADIGNACWFHHHFTDDIQTREYRAVEVILGAGYDETADVWSAACLFWEVATGDYLFDPHLTREADASQDEAHIANIIETCGRIPEELISYGDYASAIFEGRELRNVKDLRPRSLTNVLIDRYRWPDKDAEEFVAFLMPMLQTDPRLRVSAANAMHHKWLKLEKDDRDEVTDKKESKLSSGQEETTS

Target ORF information:

RefSeq Version	XM_002075243.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK17168), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002075243.2

ORF Insert Sequence:

ATGTCAATGG AACATAATTT CGCAAGCAAT CGCAATGGAA TTGACGATGA GGTTGACGTT 
GAGGAATTCT GTAGCTTCAC TTCAAAGTCA TCTGCGGCAG AAGTAGACGA GGAACATAGT 
CAAAATACTA ATGGCGAGGA AAGTCCAAGC GAATATCGCG TGGGTGGCTA TCATCCTATT 
GCTATTGGGG ATATCTTTCA GAATCGTTAT TACACATTGC GAAAACTTGG TTGGGGACAT 
TTCTCTACAG TTTGGCTATG CTACGATGCG AGATGTGAAC GCTATTGCGC CATAAAGGTG 
GTAAAGTCGG CAGAGCATTT CACCGAAACG GGACGAGATG AGATACGTTT GTCCCGCACC 
ATATCCAATC GTAATTGGCA TCCATTGCGT CAACGGTTGA TCGAATTGAT AGATTTTTTC 
TATATAAGCG GTCCGAATGG TACCCATTTG TGTCTAGTCT TTGAGGCATT GGGCGAAAAT 
CTCCTGAGTT TGATTCAACG TTCCCGCTAT CAGGGCTTGC CACTGTGGAA TGTTAAGCAG 
ATTGCCAAAC AGGTATTAGA GGGCCTATGC TTTTTGCACA CCCAATGCTC CATAATACAC 
ACCGATTTGA AGCCCGAGAA CGTGCTCCTG ATGGTTGATG ATTACACATT TCGTACGCAG 
GCGAATGATG CAGCTGAAAC TAGTAATAGC AATAGAAGTA GACAGCATAA TAATGGCATA 
ACTGCTACAA TGATGCGTCC CTCCTGCCCG CACAATGAGG AGGGGGAGGA GGGAGGTGAT 
CATCGCAACA GCGATTCCAA ACTCACAAAA ACGGCTAAAA GACGTCTACG TTCGAAAATC 
AAGCGGAGTA TTAACTTTTT TCAATCTCAT CGGCTTATGT TGCGACAACG TGGCATTGAC 
GATCTACTTC ATCTGGCCAC CAGGGGCCTG TTGACCCCCA CTATGGCTGC CTCGTGTGTG 
CGTGGCAATT TATCATATTT GCCCTTCACA TTCGATAATC TGATCATATT AGATGACAAG 
GACATGGAAC AATTGAAGAA ATTGACCATG ATCGAACGAG TTGGTGATAT GCCATCGAAA 
GATGCAATCG AAGAGAAACC CAGTTCTTCG AGTCGTACAA CATTGTTGAA AAAACATCTG 
CTTAAATGCG AGAATTCTCG AAAAAATGAA AGCTTTAAAC ATTTGGAATC ATGCTCGAAA 
ATGTTAAAAC TTCTTATGAC CAGTCCGGAG AAATTTATGC GATATGTGAA AAAGCGTATA 
GAGGAAAAGA CCAAGTCGTC GGTGCATAAG CTCCCTTCTG CAATTCCTAG ACTGAAACAA 
TCTCCAAGGA ATGGACTAGC CGCCAACAGC ACCGCAACCT TCAGTGAATT CTTTCCATTG 
AGTGCACATA ATAAGGAGTT ACCCGAACCC GGTATCAATT TAATATCACG CAAGGATCCT 
GCCACAGAGC CGTGTAAATT GAGTGTTAAG ATCGCCGATA TTGGTAATGC CTGCTGGTTC 
CATCACCATT TCACCGATGA TATACAAACC CGGGAATATC GTGCCGTCGA AGTCATTTTG 
GGTGCCGGCT ATGATGAAAC GGCGGACGTA TGGAGTGCTG CATGTTTGTT TTGGGAAGTG 
GCAACCGGTG ATTATCTCTT TGATCCGCAC TTAACGCGTG AGGCAGACGC CTCACAGGAT 
GAGGCCCACA TAGCAAATAT CATCGAGACC TGCGGCCGTA TACCTGAAGA ACTAATTAGT 
TATGGCGACT ATGCATCTGC AATTTTTGAA GGTCGCGAAT TGCGAAACGT GAAAGATTTG 
CGTCCACGAT CCTTGACTAA TGTGCTAATC GACCGCTATC GTTGGCCCGA CAAGGATGCC 
GAAGAGTTTG TTGCATTTCT AATGCCCATG CTTCAAACAG ATCCCAGGCT TCGGGTTAGT 
GCTGCCAATG CTATGCACCA CAAGTGGTTA AAGCTGGAAA AGGATGATCG GGATGAGGTT 
ACTGATAAAA AGGAGTCCAA GTTGAGCTCC GGCCAAGAAG AAACTACTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK17168 ( XM_023181357.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023037125.1 Drosophila willistoni protein kinase dsk1 (LOC6652966), mRNA.

Price & Availability↑

CloneID	ODf28409
Clone ID Related Accession (Same CDS sequence)	XM_023181357.1
Accession Version	XM_023181357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	protein kinase dsk1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCCT CGGCGCTCAG GTTAGGCATT GATCGTATTG CCAAAATGTC AATGGAACAT 
AATTTCGCAA GCAATCGCAA TGGAATTGAC GATGAGGTTG ACGTTGAGGA ATTCTGTAGC 
TTCACTTCAA AGTCATCTGT AACGTCACCA CCAAGTCAAA TGGATCATAG GATGAGTTAT 
TATGATTGTG AAGAGGATAC ACAGCAAATG CCAATGATAG AAGAAGAGGA GAATGAGGAG 
GGAGAGGAGG AGCAGCAACA GCAGCTACAG AATGAGAAGT TGATGGCGGT GAAGGAGGAG 
GAGGAAGAGG AAGATGCGAA TTTGGAACTT TACCATCGAC GATCCAAGTC GCTGGAGAAT 
TTTCACTTAA CACAGACTAA TGCAACCGAA TCAACCGAAT TCCATGAACA TAGCCAGAGA 
AAAAATTATG GAGATGATGG TGACGACGAC GACGGCAATG ACGACCATGA TAACCATGAT 
GACCATGATG ACCAAAATGA TAATGATGAC CATGACGACC ATGACGACCA TGACGACCAT 
GATGATGATG GTGAGGCGGC AGAAGTAGAC GAGGAACATA GTCAAAATAC TAATGGCGAG 
GAAAGTCCAA GCGAATATCG CGTGGGTGGC TATCATCCTA TTGCTATTGG GGATATCTTT 
CAGAATCGTT ATTACACATT GCGAAAACTT GGTTGGGGAC ATTTCTCTAC AGTTTGGCTA 
TGCTACGATG CGAGATGTGA ACGCTATTGC GCCATAAAGG TGGTAAAGTC GGCAGAGCAT 
TTCACCGAAA CGGGACGAGA TGAGATACGT TTGTCCCGCA CCATATCCAA TCGTAATTGG 
CATCCATTGC GTCAACGGTT GATCGAATTG ATAGATTTTT TCTATATAAG CGGTCCGAAT 
GGTACCCATT TGTGTCTAGT CTTTGAGGCA TTGGGCGAAA ATCTCCTGAG TTTGATTCAA 
CGTTCCCGCT ATCAGGGCTT GCCACTGTGG AATGTTAAGC AGATTGCCAA ACAGGTATTA 
GAGGGCCTAT GCTTTTTGCA CACCCAATGC TCCATAATAC ACACCGATTT GAAGCCCGAG 
AACGTGCTCC TGATGGTTGA TGATTACACA TTTCGTACGC AGGCGAATGA TGCAGCTGAA 
ACGTATTTGA AGAATCATGC CCATCTTTAC AATAGCACAG CAAATGGCAA TATTGGTAAT 
GCTAATTGTA GTAAATGTAA TAGTAGTAAT AGCAATAGAA GTAGACAGCA TAATAATGGC 
ATAACTGCTA CAATGATGCG TCCCTCCTGC CCGCACAATG AGGAGGGGGA GGAGGGAGGT 
GATCATCGCA ACAGCGATTC CAAACTCACA AAAACGGCTA AAAGACGTCT ACGTTCGAAA 
ATCAAGCGGA GTATTAACTT TTTTCAATCT CATCGGCTTA TGTTGCGACA ACGTGGCATT 
GACGATCTAC TTCATCTGGC CACCAGGGGC CTGTTGACCC CCACTATGGC TGCCTCGTGT 
GTGCGTGGCA ATTTATCATA TTTGCCCTTC ACATTCGATA ATCTGATCAT ATTAGATGAC 
AAGGACATGG AACAATTGAA GAAATTGACC ATGATCGAAC GAGTTGGTGA TATGCCATCG 
AAAGATGCAA TCGAAGAGAA ACCCAGTTCT TCGAGTCGTA CAACATTGTT GAAAAAACAT 
CTGCTTAAAT GCGAGAATTC TCGAAAAAAT GAAAGCTTTA AACATTTGGA ATCATGCTCG 
AAAATGTTAA AACTTCTTAT GACCAGTCCG GAGAAATTTA TGCGATATGT GAAAAAGCGT 
ATAGAGGAAA AGACCAAGTC GTCGGTGCAT AAGCTCCCTT CTGCAATTCC TAGACTGAAA 
CAATCTCCAA GGAATGGACT AGCCGCCAAC AGCACCGCAA CCTTCAGTGA ATTCTTTCCA 
TTGAGTGCAC ATAATAAGGA GTTACCCGAA CCCGGTATCA ATTTAATATC ACGCAAGGAT 
CCTGCCACAG AGCCGTGTAA ATTGAGTGTT AAGATCGCCG ATATTGGTAA TGCCTGCTGG 
TTCCATCACC ATTTCACCGA TGATATACAA ACCCGGGAAT ATCGTGCCGT CGAAGTCATT 
TTGGGTGCCG GCTATGATGA AACGGCGGAC GTATGGAGTG CTGCATGTTT GTTTTGGGAA 
GTGGCAACCG GTGATTATCT CTTTGATCCG CACTTAACGC GTGAGGCAGA CGCCTCACAG 
GATGAGGCCC ACATAGCAAA TATCATCGAG ACCTGCGGCC GTATACCTGA AGAACTAATT 
AGTTATGGCG ACTATGCATC TGCAATTTTT GAAGGTCGCG AATTGCGAAA CGTGAAAGAT 
TTGCGTCCAC GATCCTTGAC TAATGTGCTA ATCGACCGCT ATCGTTGGCC CGACAAGGAT 
GCCGAAGAGT TTGTTGCATT TCTAATGCCC ATGCTTCAAA CAGATCCCAG GCTTCGGGTT 
AGTGCTGCCA ATGCTATGCA CCACAAGTGG TTAAAGCTGG AAAAGGATGA TCGGGATGAG 
GTTACTGATA AAAAGGAGTC CAAGTTGAGC TCCGGCCAAG AAGAAACTAC TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023037125.1
CDS	128..2704
Translation	MSSSALRLGIDRIAKMSMEHNFASNRNGIDDEVDVEEFCSFTSKSSVTSPPSQMDHRMSYYDCEEDTQQMPMIEEEENEEGEEEQQQQLQNEKLMAVKEEEEEEDANLELYHRRSKSLENFHLTQTNATESTEFHEHSQRKNYGDDGDDDDGNDDHDNHDDHDDQNDNDDHDDHDDHDDHDDDGEAAEVDEEHSQNTNGEESPSEYRVGGYHPIAIGDIFQNRYYTLRKLGWGHFSTVWLCYDARCERYCAIKVVKSAEHFTETGRDEIRLSRTISNRNWHPLRQRLIELIDFFYISGPNGTHLCLVFEALGENLLSLIQRSRYQGLPLWNVKQIAKQVLEGLCFLHTQCSIIHTDLKPENVLLMVDDYTFRTQANDAAETYLKNHAHLYNSTANGNIGNANCSKCNSSNSNRSRQHNNGITATMMRPSCPHNEEGEEGGDHRNSDSKLTKTAKRRLRSKIKRSINFFQSHRLMLRQRGIDDLLHLATRGLLTPTMAASCVRGNLSYLPFTFDNLIILDDKDMEQLKKLTMIERVGDMPSKDAIEEKPSSSSRTTLLKKHLLKCENSRKNESFKHLESCSKMLKLLMTSPEKFMRYVKKRIEEKTKSSVHKLPSAIPRLKQSPRNGLAANSTATFSEFFPLSAHNKELPEPGINLISRKDPATEPCKLSVKIADIGNACWFHHHFTDDIQTREYRAVEVILGAGYDETADVWSAACLFWEVATGDYLFDPHLTREADASQDEAHIANIIETCGRIPEELISYGDYASAIFEGRELRNVKDLRPRSLTNVLIDRYRWPDKDAEEFVAFLMPMLQTDPRLRVSAANAMHHKWLKLEKDDRDEVTDKKESKLSSGQEETTS

Target ORF information:

RefSeq Version	XM_023181357.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni protein kinase dsk1 (LOC6652966), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023181357.1

ORF Insert Sequence:

ATGTCTTCCT CGGCGCTCAG GTTAGGCATT GATCGTATTG CCAAAATGTC AATGGAACAT 
AATTTCGCAA GCAATCGCAA TGGAATTGAC GATGAGGTTG ACGTTGAGGA ATTCTGTAGC 
TTCACTTCAA AGTCATCTGT AACGTCACCA CCAAGTCAAA TGGATCATAG GATGAGTTAT 
TATGATTGTG AAGAGGATAC ACAGCAAATG CCAATGATAG AAGAAGAGGA GAATGAGGAG 
GGAGAGGAGG AGCAGCAACA GCAGCTACAG AATGAGAAGT TGATGGCGGT GAAGGAGGAG 
GAGGAAGAGG AAGATGCGAA TTTGGAACTT TACCATCGAC GATCCAAGTC GCTGGAGAAT 
TTTCACTTAA CACAGACTAA TGCAACCGAA TCAACCGAAT TCCATGAACA TAGCCAGAGA 
AAAAATTATG GAGATGATGG TGACGACGAC GACGGCAATG ACGACCATGA TAACCATGAT 
GACCATGATG ACCAAAATGA TAATGATGAC CATGACGACC ATGACGACCA TGACGACCAT 
GATGATGATG GTGAGGCGGC AGAAGTAGAC GAGGAACATA GTCAAAATAC TAATGGCGAG 
GAAAGTCCAA GCGAATATCG CGTGGGTGGC TATCATCCTA TTGCTATTGG GGATATCTTT 
CAGAATCGTT ATTACACATT GCGAAAACTT GGTTGGGGAC ATTTCTCTAC AGTTTGGCTA 
TGCTACGATG CGAGATGTGA ACGCTATTGC GCCATAAAGG TGGTAAAGTC GGCAGAGCAT 
TTCACCGAAA CGGGACGAGA TGAGATACGT TTGTCCCGCA CCATATCCAA TCGTAATTGG 
CATCCATTGC GTCAACGGTT GATCGAATTG ATAGATTTTT TCTATATAAG CGGTCCGAAT 
GGTACCCATT TGTGTCTAGT CTTTGAGGCA TTGGGCGAAA ATCTCCTGAG TTTGATTCAA 
CGTTCCCGCT ATCAGGGCTT GCCACTGTGG AATGTTAAGC AGATTGCCAA ACAGGTATTA 
GAGGGCCTAT GCTTTTTGCA CACCCAATGC TCCATAATAC ACACCGATTT GAAGCCCGAG 
AACGTGCTCC TGATGGTTGA TGATTACACA TTTCGTACGC AGGCGAATGA TGCAGCTGAA 
ACGTATTTGA AGAATCATGC CCATCTTTAC AATAGCACAG CAAATGGCAA TATTGGTAAT 
GCTAATTGTA GTAAATGTAA TAGTAGTAAT AGCAATAGAA GTAGACAGCA TAATAATGGC 
ATAACTGCTA CAATGATGCG TCCCTCCTGC CCGCACAATG AGGAGGGGGA GGAGGGAGGT 
GATCATCGCA ACAGCGATTC CAAACTCACA AAAACGGCTA AAAGACGTCT ACGTTCGAAA 
ATCAAGCGGA GTATTAACTT TTTTCAATCT CATCGGCTTA TGTTGCGACA ACGTGGCATT 
GACGATCTAC TTCATCTGGC CACCAGGGGC CTGTTGACCC CCACTATGGC TGCCTCGTGT 
GTGCGTGGCA ATTTATCATA TTTGCCCTTC ACATTCGATA ATCTGATCAT ATTAGATGAC 
AAGGACATGG AACAATTGAA GAAATTGACC ATGATCGAAC GAGTTGGTGA TATGCCATCG 
AAAGATGCAA TCGAAGAGAA ACCCAGTTCT TCGAGTCGTA CAACATTGTT GAAAAAACAT 
CTGCTTAAAT GCGAGAATTC TCGAAAAAAT GAAAGCTTTA AACATTTGGA ATCATGCTCG 
AAAATGTTAA AACTTCTTAT GACCAGTCCG GAGAAATTTA TGCGATATGT GAAAAAGCGT 
ATAGAGGAAA AGACCAAGTC GTCGGTGCAT AAGCTCCCTT CTGCAATTCC TAGACTGAAA 
CAATCTCCAA GGAATGGACT AGCCGCCAAC AGCACCGCAA CCTTCAGTGA ATTCTTTCCA 
TTGAGTGCAC ATAATAAGGA GTTACCCGAA CCCGGTATCA ATTTAATATC ACGCAAGGAT 
CCTGCCACAG AGCCGTGTAA ATTGAGTGTT AAGATCGCCG ATATTGGTAA TGCCTGCTGG 
TTCCATCACC ATTTCACCGA TGATATACAA ACCCGGGAAT ATCGTGCCGT CGAAGTCATT 
TTGGGTGCCG GCTATGATGA AACGGCGGAC GTATGGAGTG CTGCATGTTT GTTTTGGGAA 
GTGGCAACCG GTGATTATCT CTTTGATCCG CACTTAACGC GTGAGGCAGA CGCCTCACAG 
GATGAGGCCC ACATAGCAAA TATCATCGAG ACCTGCGGCC GTATACCTGA AGAACTAATT 
AGTTATGGCG ACTATGCATC TGCAATTTTT GAAGGTCGCG AATTGCGAAA CGTGAAAGAT 
TTGCGTCCAC GATCCTTGAC TAATGTGCTA ATCGACCGCT ATCGTTGGCC CGACAAGGAT 
GCCGAAGAGT TTGTTGCATT TCTAATGCCC ATGCTTCAAA CAGATCCCAG GCTTCGGGTT 
AGTGCTGCCA ATGCTATGCA CCACAAGTGG TTAAAGCTGG AAAAGGATGA TCGGGATGAG 
GTTACTGATA AAAAGGAGTC CAAGTTGAGC TCCGGCCAAG AAGAAACTAC TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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DWIL\GK17168 cDNA ORF clone, Drosophila willistoni

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