LOC6649543 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6649543
Entrez Gene ID	6649543
Full Name	titin
Synonyms	GK22633,GK22633-PB,GK22633-PC
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_023179838.1	XP_023035606.1	uncharacterized protein LOC6649543 isoform X1
XM_023179839.1	XP_023035607.1	uncharacterized protein LOC6649543 isoform X2
XM_002072004.2	XP_002072040.2	uncharacterized protein, isoform C
XM_023179841.1	XP_023035609.1	uncharacterized protein LOC6649543 isoform X4
XM_023179842.1	XP_023035610.1	titin isoform X5
XM_015177070.1	XP_015032556.1	uncharacterized protein, isoform B
XM_023179840.1	XP_023035608.1	uncharacterized protein LOC6649543 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6649543 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6649543 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODf27217	XM_023179842.1 Latest version!	Drosophila willistoni titin (LOC6649543), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODf16565	XM_002072004.2 Latest version!	Drosophila willistoni uncharacterized protein, transcript variant C (Dwil\GK22633), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODf16725	XM_015177070.1 Latest version!	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK22633), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODf27213	XM_023179838.1 Latest version!	Drosophila willistoni titin (LOC6649543), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODf27214	XM_023179839.1 Latest version!	Drosophila willistoni titin (LOC6649543), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODf27215	XM_023179840.1 Latest version!	Drosophila willistoni titin (LOC6649543), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODf27216	XM_023179841.1 Latest version!	Drosophila willistoni titin (LOC6649543), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6649543 ( XM_023179842.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023035610.1 Drosophila willistoni titin (LOC6649543), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODf27217
Clone ID Related Accession (Same CDS sequence)	XM_023179842.1
Accession Version	XM_023179842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	titin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032872.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTT ACTTGTTGGA ACGTGTTTTG ATAGTGCGCG ATCGTTTGCA ACGGCATCAG 
GATGTGCTCT GTGAATTTGT CAATGCTGTT CTTATAGTGG AAAGTAACGA TGCACAGCCT 
AAAATGCGTT TTAGCCTTTC ACCACCGCCG CAGAAAGCTA TTACCGCCAG CGTAACCTCA 
ACCAAGAGTA GCACAAACAT TGACATTAGC TCCAGCTCCA GCACCATCAC CACCGCGGCT 
ACAACCAGCA CCACCAAAAC AACAACAACC ACTTTCTCCA CCACCACCAC CAACAACAAA 
AATAATATGC TGGGTCATGA GGGGAACAGC TCAAATTCAG CTGCCTCTAT TTCTGCATCA 
GCTGACGAAG ATGCCGCCTC GGATTATAAT CAATGGCTGC ATGCCATGAA ACTGGTGGCT 
CGTCTACCAG GAGGCACACC ACCCGAGTTT CGTCGCAAGC TCTGGCTCTC GTTGGCTGAC 
AAATATCTCA AGTCCAAGAA TGTTGATTGG ACGCAGCAGC GTGAGAAATG CTTTTGCGAG 
GAATGGCGTG AAGATGACGA AGAGCTGGGC ATACAAATTG TCAAGGATTT GCATCGCACT 
GGTTCTAATT TGTGCACTGG TCCCGCGGGC TCCATTAACC AGGCCAAGCT GAAGCGCATT 
CTACTCGGCT ATGCCCGCTA TAATCCCGAG GTGGGCTATT GTCAGGGCTT CAATATGCTG 
GGTGCTTTGA TTCTACAAGT CATGGACAAG GAAGAGGAGG AGTCCATGAA GGTCATGATC 
TATCTGGTGG AGGGTGTACT GCCAACGGGT TATTTCTATG GTTCAATGGG CGGTCTTCAG 
GCGGATATGG GAGTCTTTCG CGAGCTTATG CAAACGAAAC TGCCGCGTCT GGCCAAGCAT 
TTGCAGCGTT TGCAGGGAGG ACCCGTTGAG AATGCCTACG AACCGCCATT GACCAATGTC 
TTCACCATGC AATGGTTCCT CACCATGTTC TGCACCTGCC TACCCATGTC CTGTGTCCTG 
CGCGTCTGGG ATCTCGTTCT CATCGAAGGC AGTGATGTTC TATTGCGCAC TGCCCTCGTC 
CTGTGGAGTC TATTGGAAGA ACGTGTCCTT AATGCTCGCA CAGCTGATGA GTTCTATGGC 
AAAATGGGAT CTTTCTCGAG TGAATTGCTC AATGGTCATC TTATCGATTC AAATGGTCTA 
ATCGAAAAGG TTGTTAAACT CGGACCCATT GCCGATTTGC GTCATTTGAG GGATAAACAT 
CTATATAACA TAGCCCCATT GAGGCATAAA CAAGGCATAC AACTCTATTA CGATGACGAT 
GATACACATT CGGATGAGGA GCGCATGGCA GTGGCTACCG TGTGGGGTTT AAATTGGGGT 
CGCCGCGGTT CGGTGGGACC CGGAGCCCCA GCGGGTAGGC AGCCATCTGA ACAAAAGGAT 
CGTCTGGCCT TGGATATATC GTTGCTGAAG AAGCAATACG ATCGTCTTCG AGAACGTCAG 
AAGCAGGCCC ATGTCATCCT AACTACTGCA TGCTCAACAG CAACTCGTCA AACGCCTTCA 
GCGCAAAGCT CGGTGCCGGT TAATCAGTTA CTACAGGGTC GACACGCCAT TGTGACCAAC 
AGGGGCAAAC GGGTGAGTGC TCCCTTGGGA GCCATACCTC CAGCCCGAAA GCCTTCACTG 
CCAGCTGTAT TGCACGGAAA CGGAAAGCCT ATGATGGGGG AGAAGCAGTT GCGACGAGGA 
GAAACCCTAC TCTGGCGGGA CACGGACACT AGTCGACGGC GAAGAGACAG CCTCACGTGG 
AAGGAGATCA AAGCAGATCG TGCGGCCATG CTACGCGAAG GAGTTGATTC AAGCTCTCTA 
AAGACCCAGA AACTTCGCAC GCGGTTGGGC AAAAGTGACA GTTCGTCTTA CAGTGAGGAT 
AGCGATGGCG ATCAGGATGA GAAAGCAGAC AACGGTGGTG CTGGTGGTGG CTCTAGTACG 
GACACCAGTC TCTGTGATGA CGATGATCCC AAGTCAAGCG AAAAAAGCCC CAAGCGTAAG 
GCGAAGGTGG CACGTAAGCT CAAAGAGAAG AGTCAGCATT TGGGGTCTCG GGAGTCCAGC 
GCAGAGCGTC AACGACCGAA ATCCTGGGCA CCAGGCACGC ATGAGATACC CTTTATGTTG 
ATGGGCATGG ACAGCGGTGA TGAAAAGGAA GCCAAAAAGG AGGAGCAAGA CCTGGAGGAA 
GACAGTGCCA CTGAAAGTGG TCGTTTTAGC TATCCTAAGG AACTAGATTG GACTCAGGCG 
GCAGATAAAG TAGCAAGTTG TGAACTAGAA CCATTGGATA TTGACAAGAT ACTGCCTAAC 
ACTGCTATTT ATCTAAGCAC AATCAGTCTA AGTCCGCCCA AGACGGAAGA TGTGGGAGAT 
CAGGATGTGA TAGAGGAACA ACCCATTGTG AAGCAGTTTG ACGTTAGTGA TTCTGGGGTA 
ACAAATCAAT ATTTTGAGCG TGTCAACAGC GTTGAGCGTC CCAATAGGTT GGAGCTCTCA 
TACTCTCTAA ACGAGGAGGA GGACTCTACA ATGTTAAAAG ACGATCGTCA AAGTTCGGAT 
TTTGAATTGA AAATTGAACC CGAACCTTCC ACTTTAAATA AGGTCCCACA AATTCGGGAT 
GATAACATAC CCGGTGAAAA CAAAGATGAC TACAAGGAGC TTTTGAGCAT GACGATAGAA 
GAGCATAAGC CACCAGCAGC TACAATCACC AGCCAACTGA ATGCGAGTCG AAAGCGTCGG 
GATCCCAGGC GTAAAACGCT AACCAGATCC TCCACAATCG AGATTGAGGA ACGTTTTCAG 
GCCCTCGAAC GAACACTGAG CCAAGATCAA CCGATTCATA AGTACATACC GAGCACTGCA 
GCTCTCGAAG AACGCTATAA TAGTCTAGAA AAACAGTTGA GTGCCGAGAA ACAGCGGAAG 
GAGGAGGATC TGGATCGTAT ACCTTCTACA GCTGATCTAG AATCACGATT TGATGCCTTA 
ACGAAACAAA TGAGTTCCAG CGAATCGAGC AATTCCAAGG CCCCACTCGA TCTTAATAAA 
GATCAGCAGC AGCCTGGCGA GAAATCCAAA GAAACAGAAA GAAAATCAAA CAAAAACCAA 
AGCAAAGAAG GGGAGAAACA AACCCAATCG GAACCTAAAG AAACTGCATC GGAAAAGGAA 
AGTCCTGGCC AAAAAGAAGA AACCAAAGAA ATTGAAGAAG AACAACAGCC ACGTCTCAAG 
AAACTGCCCT CTACAGCCGA ACTTGAGGAT CGATTTAATG CTTTAGAACG AAAAATGAGC 
GTACAAAAAT CTAGTTCGGC AAAGACAAAG AAAGAGCCAC CCGATGAAGA AAAACCTGCC 
CAAAAAACTG AAGCTCAAAC AAAGGAAACT AAATCTAAAC AAGAATCAAG CCCACAAAAG 
ACTCCAGAAA ACATAGAGAC AAGTGAAAAG AGCACGAAAA TCAGTAAGGA TTCAAATGAG 
GTTAAGGATA AAGCCGATGA GGTAAAACGA AAAGTTCCAC CTTCAACCGA GGAGTTGGAA 
AAGCGTTTCA ATGCTTTGGA GAAACAACTT AGTACAACTA ATTTGGAAGC TGAGCAACCT 
ACTACTTCTA AGGCAAAGGA AATCAAATCA GAATTACCCA AGCAAACTGA AAATGTAAAA 
GCGAACAAGC AAGAAATTCA ATCACAAAAA TCCATCAAGT CTTTCGATGA AAAAGTTAAA 
GTTATTAATA CAAAATTAAC AGCCGATGAG CAGAAAATCC AAGACCACGA CAAATTGGCC 
GAAGAAAAAC GTAAAAGTCT TGATGAGAAA AGTGAAAGAG CCAGTGGTCA GTTAAAAGAA 
ATGTTGCCTG AGAATGAAAC CATTAAATCA AAGACATCTC AACCTGAAGT CGTCCGGGCT 
GATGTTCAAC CCAAAGAACT GGGTAAGCGA AGAGCTTCCG AACCACCATC AACAGAAGAT 
TTGGAGAAAC GCTATGAGAC ACTTAAGCGT CGCATGAGTA GTAAAAATCA TTTTGGTAAT 
CTAACAGAAT CAGCAGATGA GTCCATGTCA AGGCGAGATC AAGAAGTAAT ATCCGAGACT 
TTAGAGACTG GAGAGCCGGA GAGAAAACAA TCACCGCCGA CAACAGAATA TCTGGAAAGT 
AGGTATGAGA AGCTACATGG GGAAAACAAG AAAACAACGC CCAAGAAAGT GGATGTGGCG 
ATTGAGGCGC ATATATCACC ACCACCAGCT CCGCCACCAC CTCCAAAGCC GAATATCCTA 
GCTGATCCGG CTATGCATAA GCAACGAGCT CTCATTGAAG AGCTACAGGC GAAAATTCAA 
GAGCAAGGAC AGTCCACGCC TGGTGAAGAG AATCTCAAGC CCAGCGAGAT AAATCCACAG 
CGCAAACAGA GGCAAGAGCA ACAACGTCTG CTACAACGGC CCACGACAAT GGGAGATGAG 
ACCTCGGAAG CACCTGCAAA GACCGCTTAC TACAGATCGG GAAATTACGA ACCCTGGCAG 
CAGCAGCACC AGCAGAAGCA GCGAATGGTT CGTCGTTTCT CTGATTTGCC ATCAAGAGCC 
GATCTAGAAA ATCGCTTACA ATTCCTGGAG AAGCAATTGT ACAAGAAATT CTACAAGCAG 
CGCTGTGCAA GTGATTCCGA AGTTGCATCG CGGACGATCG ATGACGAAAA GCCCAGCACA 
TCGGCAGCCC GTGTGGCGGA AGTTCAGCTG GAGCAACGTG TCCTGGCCCT AGAGAAGCAA 
TTAAGTGAGA ATAGTCTAAA GCTGCTAGAG GCCATGCGTG TCAACGTGCC CAACGTCGAT 
GGCGGCTCTC CGCGAAGACT AAGCACGGAT ACGATAGATG CCACTGGCAA GGAACTGGTG 
CGGTATACAC AGAACTTTGG TGAGCTGGAC GAGTTGGGTG GAGATGGCTC AAATAAGCCC 
ATTAACATTA GTATCAATAT CAAAATGATG CTCAACAAGG AACAGCAACA ACAACGACAA 
CAACAACAGC AGCAGCAGCA GCAGGAGGAA AAGGCAGACA CTAGGACCAG CACAGAGGAT 
TTAAGGATGC GCTTGGAGCA ATTGGAACAG CAATTGATGG AAGAAAGAGC CAAGAACAAT 
GGTTTTGTGG ATGTCGAAAG TGCTAATGAT GGCGGAGCAG GAGAGGAGGC AAAGCAATTG 
GATAAATCCG AAACTTCAGT ACCGGAAGAA CTAGAGGAAA ATAAAAAGCA AGAGAAGGAC 
AGTCACAATC AAACGGTAAA AAGTGATGAA GCTGAAATGA CAGAAGATCC AATTGTGGCC 
ACCACCGTGG AAACCATTGA GCCGGAGACA GTGAAAGAAA CAAAGACCTT AGAAAACGAG 
GAGATAGCTC AAGCAACCCA AGAGAAAGCT GCAAAAGCTG AGGAGATCGA AGAGACAACC 
AAAGATAAAA TAAATGAATC ATCTGAAGTC ATTCCTAAAG TGGAGGAAAC TAAACCGCCA 
ACAGAAACAG AAACCCACTG CGAAGAGAAA ACTGTAGACT CTGACAAGAT TATTGTCAAT 
GCCAGCTCTA TTGAACCTCC GGTGGATCCC AATAATAAGA CCGTGGTCCT GCTTATGGAT 
AACGAACCTA GAGCTTCGAG AGTGCGCCGT TTGACCCGCG CCAATACCGA AGAACTGGAA 
GGTCTATTCC AGGCTTTAGA AAAACAACTG CAGGATCGCA ATCTAATCAA ATCTGAAGAT 
GGGCGGTTAA TACGAGCAAG TACGGAACAG AAACAAACTG CAGAACAAGT AGAACAAGCT 
CAGGCTATAA GCGATCTAAC TAAGGAAATC GAAGACTTTA CCAGCGGTAA ACCAGACGCA 
AAAAGTGAAG GCGAAACAAA GGAAGATAAG CCAGCAGATG GCGGCGAAGA ACCCCCCGAC 
GATTATGATT GGGGTCCCAA TCCGGTTAAG CATCATTTGA AACGCAAAAC CGTATATTTG 
CCTTCAACCA AAGAGCTAGA GGCTCGTTTT CGGTCACTGG AACGTCAAAT TAAACTACTC 
GAAGATGTGG AGAAAATCGA TGTGGAGCAG CGCTTAAATG AAATTGAACG AAAGATTAAA 
CTGCAGTATT CCTTGTCACA TGAGAAAGAT TTAAATAAAT ATCTAGAGCT CTGCGAAGGC 
AAGGGCTTAG ATGAGGAAGA GGAGCCTATT GTAGATGCAT CTCCTCCTTT AACCAAGGAT 
ACCCAGCGGT CCCGTAGTCC AGCACGTAAA GAAGCTTCAA AGTCGCCATA TACTTCACCA 
TCGCGTAAGG CAGTTGCAAA ATCTCCTCAT ACTTCTCCTG CACGTAAGCC AGCTACAAAA 
TCTCCTTATA CCTCGCCTTC CCGTGCTAGT GACAAAAAGC GGAGCCCTTA TACCTCGCCA 
GTACGTCGAC AGGCGCATCC CGGTGATTTA CCCATCTCCG ATGACTTGGA GTACAAGTAT 
CGTGTGCTTG ATCTGGTACG TTCCAAATCC AAGGAGAACT TGTCGAAGCG TAAAACTGAT 
CCCAATAAAA AGCCGCCCAT TCATCCTCTT GAAATGCTAC TTGATCCGAG TCCCGATGAC 
AGCGAGATAC CCACAACAGG GGAACTGGAG CACCGCATAC GTCTGCTGGA TGAAAAGCTA 
AAGTCGCCCT GCCGCCATAA ATCTCGCTCA CGTTCACCCA CCATCGATGA CATGAAACGA 
AAGAAGCTCT TGGAGGAAAA GCGACCCAAA TCGCCGGTTC ATACATTGGA ACGTTTGGTC 
AGTTCACCTA ACAGACCAGA GCCACCCACT GAGGAGGAAC TAGAACAGCG AATGCGTGCC 
TTGGAAGACG AAAAGCGTTT CGACTTCAAG ACCCAAAAGG ATTACAAGGC ATTCAATCAA 
AAGCTTAAAG ATGTCATCTC ACCTTCCCTA TCTTTCGAAG AGTTCAAGGC GGCCAAATCG 
CGTGAACAGA GTCCACGGCG TCAAGGAGCC ACAACACCCA AATCAGCAAT GCGACGCGAC 
GATACAGATG ATTACCGAGG ACGCGAGGAG ACACCCCATT ATCGGCCCAC TAGTCCTAAG 
GTGATACGCT TTCGAGATGA GGATGAGGAT TATTACAAGG AGAGCCAGCG TCCGAAGTCT 
CGACAGTCCA GCGAACGAAT GACTCTTGCA GATCGCAGCT ACAGCAGCAG CATTGCAGCA 
GCAACGGCCA GCAGCTCCGA GGGTCTCGAT GCCCTGGGTA GTCGCCTAAT GAGAGAAACC 
TCGCCAATTA CCCGCACTGG GACTCATACT GGTGTGCCGT TACGGACTGG AGAGAATATC 
AATGATCGTC TAAGTTCTAT CAAGGATACC ATCAAATCCA TTGACACTCT ATGCGAGGAG 
AAACCGTATC AGAAGGAGAA ATGCCAACGT TATATTGATT CCCTCTTCTC AGATTCATTG 
CATTTCGGTT GTAAGAAGAA TTCCATGGAG GAGCTAACGC ACAGTCGCAG CGAGAGTCGA 
GGACGTACAG CACAACGATC GAGTGACTAT ACGCCGGCCA TTCGAGTCAC CTCAGAGCAT 
CGATCTCTGG GCTCGGCCGA CTCGAAGCGC ACAAGTCCAT TGCGGGCATC CAGTCCGTTG 
CATCATCATC GCTCGCATAG GGATATCAGT AGGGAGTTAT CGCCGCGCAG GCGTCGTGAT 
GCCGAGGATA TGGAGGAGCG CGAAAGTAGT AGGTTGTTGA ATTTCAATTA TGCCATTACA 
TCAAGTTCGA AACATAAGAA AATTATTAAT TCACATTCCA ATGGCTATAT AAATTATTAC 
ATCAAGCAAA GGAACAGAAA ATTAATCAAA ACGATTAGAA AAATATTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023035610.1
CDS	45..7835
Translation	MMGYLLERVLIVRDRLQRHQDVLCEFVNAVLIVESNDAQPKMRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRLLNFNYAITSSSKHKKIINSHSNGYINYYIKQRNRKLIKTIRKIL

Target ORF information:

RefSeq Version	XM_023179842.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni titin (LOC6649543), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023179842.1

ORF Insert Sequence:

ATGATGGGTT ACTTGTTGGA ACGTGTTTTG ATAGTGCGCG ATCGTTTGCA ACGGCATCAG 
GATGTGCTCT GTGAATTTGT CAATGCTGTT CTTATAGTGG AAAGTAACGA TGCACAGCCT 
AAAATGCGTT TTAGCCTTTC ACCACCGCCG CAGAAAGCTA TTACCGCCAG CGTAACCTCA 
ACCAAGAGTA GCACAAACAT TGACATTAGC TCCAGCTCCA GCACCATCAC CACCGCGGCT 
ACAACCAGCA CCACCAAAAC AACAACAACC ACTTTCTCCA CCACCACCAC CAACAACAAA 
AATAATATGC TGGGTCATGA GGGGAACAGC TCAAATTCAG CTGCCTCTAT TTCTGCATCA 
GCTGACGAAG ATGCCGCCTC GGATTATAAT CAATGGCTGC ATGCCATGAA ACTGGTGGCT 
CGTCTACCAG GAGGCACACC ACCCGAGTTT CGTCGCAAGC TCTGGCTCTC GTTGGCTGAC 
AAATATCTCA AGTCCAAGAA TGTTGATTGG ACGCAGCAGC GTGAGAAATG CTTTTGCGAG 
GAATGGCGTG AAGATGACGA AGAGCTGGGC ATACAAATTG TCAAGGATTT GCATCGCACT 
GGTTCTAATT TGTGCACTGG TCCCGCGGGC TCCATTAACC AGGCCAAGCT GAAGCGCATT 
CTACTCGGCT ATGCCCGCTA TAATCCCGAG GTGGGCTATT GTCAGGGCTT CAATATGCTG 
GGTGCTTTGA TTCTACAAGT CATGGACAAG GAAGAGGAGG AGTCCATGAA GGTCATGATC 
TATCTGGTGG AGGGTGTACT GCCAACGGGT TATTTCTATG GTTCAATGGG CGGTCTTCAG 
GCGGATATGG GAGTCTTTCG CGAGCTTATG CAAACGAAAC TGCCGCGTCT GGCCAAGCAT 
TTGCAGCGTT TGCAGGGAGG ACCCGTTGAG AATGCCTACG AACCGCCATT GACCAATGTC 
TTCACCATGC AATGGTTCCT CACCATGTTC TGCACCTGCC TACCCATGTC CTGTGTCCTG 
CGCGTCTGGG ATCTCGTTCT CATCGAAGGC AGTGATGTTC TATTGCGCAC TGCCCTCGTC 
CTGTGGAGTC TATTGGAAGA ACGTGTCCTT AATGCTCGCA CAGCTGATGA GTTCTATGGC 
AAAATGGGAT CTTTCTCGAG TGAATTGCTC AATGGTCATC TTATCGATTC AAATGGTCTA 
ATCGAAAAGG TTGTTAAACT CGGACCCATT GCCGATTTGC GTCATTTGAG GGATAAACAT 
CTATATAACA TAGCCCCATT GAGGCATAAA CAAGGCATAC AACTCTATTA CGATGACGAT 
GATACACATT CGGATGAGGA GCGCATGGCA GTGGCTACCG TGTGGGGTTT AAATTGGGGT 
CGCCGCGGTT CGGTGGGACC CGGAGCCCCA GCGGGTAGGC AGCCATCTGA ACAAAAGGAT 
CGTCTGGCCT TGGATATATC GTTGCTGAAG AAGCAATACG ATCGTCTTCG AGAACGTCAG 
AAGCAGGCCC ATGTCATCCT AACTACTGCA TGCTCAACAG CAACTCGTCA AACGCCTTCA 
GCGCAAAGCT CGGTGCCGGT TAATCAGTTA CTACAGGGTC GACACGCCAT TGTGACCAAC 
AGGGGCAAAC GGGTGAGTGC TCCCTTGGGA GCCATACCTC CAGCCCGAAA GCCTTCACTG 
CCAGCTGTAT TGCACGGAAA CGGAAAGCCT ATGATGGGGG AGAAGCAGTT GCGACGAGGA 
GAAACCCTAC TCTGGCGGGA CACGGACACT AGTCGACGGC GAAGAGACAG CCTCACGTGG 
AAGGAGATCA AAGCAGATCG TGCGGCCATG CTACGCGAAG GAGTTGATTC AAGCTCTCTA 
AAGACCCAGA AACTTCGCAC GCGGTTGGGC AAAAGTGACA GTTCGTCTTA CAGTGAGGAT 
AGCGATGGCG ATCAGGATGA GAAAGCAGAC AACGGTGGTG CTGGTGGTGG CTCTAGTACG 
GACACCAGTC TCTGTGATGA CGATGATCCC AAGTCAAGCG AAAAAAGCCC CAAGCGTAAG 
GCGAAGGTGG CACGTAAGCT CAAAGAGAAG AGTCAGCATT TGGGGTCTCG GGAGTCCAGC 
GCAGAGCGTC AACGACCGAA ATCCTGGGCA CCAGGCACGC ATGAGATACC CTTTATGTTG 
ATGGGCATGG ACAGCGGTGA TGAAAAGGAA GCCAAAAAGG AGGAGCAAGA CCTGGAGGAA 
GACAGTGCCA CTGAAAGTGG TCGTTTTAGC TATCCTAAGG AACTAGATTG GACTCAGGCG 
GCAGATAAAG TAGCAAGTTG TGAACTAGAA CCATTGGATA TTGACAAGAT ACTGCCTAAC 
ACTGCTATTT ATCTAAGCAC AATCAGTCTA AGTCCGCCCA AGACGGAAGA TGTGGGAGAT 
CAGGATGTGA TAGAGGAACA ACCCATTGTG AAGCAGTTTG ACGTTAGTGA TTCTGGGGTA 
ACAAATCAAT ATTTTGAGCG TGTCAACAGC GTTGAGCGTC CCAATAGGTT GGAGCTCTCA 
TACTCTCTAA ACGAGGAGGA GGACTCTACA ATGTTAAAAG ACGATCGTCA AAGTTCGGAT 
TTTGAATTGA AAATTGAACC CGAACCTTCC ACTTTAAATA AGGTCCCACA AATTCGGGAT 
GATAACATAC CCGGTGAAAA CAAAGATGAC TACAAGGAGC TTTTGAGCAT GACGATAGAA 
GAGCATAAGC CACCAGCAGC TACAATCACC AGCCAACTGA ATGCGAGTCG AAAGCGTCGG 
GATCCCAGGC GTAAAACGCT AACCAGATCC TCCACAATCG AGATTGAGGA ACGTTTTCAG 
GCCCTCGAAC GAACACTGAG CCAAGATCAA CCGATTCATA AGTACATACC GAGCACTGCA 
GCTCTCGAAG AACGCTATAA TAGTCTAGAA AAACAGTTGA GTGCCGAGAA ACAGCGGAAG 
GAGGAGGATC TGGATCGTAT ACCTTCTACA GCTGATCTAG AATCACGATT TGATGCCTTA 
ACGAAACAAA TGAGTTCCAG CGAATCGAGC AATTCCAAGG CCCCACTCGA TCTTAATAAA 
GATCAGCAGC AGCCTGGCGA GAAATCCAAA GAAACAGAAA GAAAATCAAA CAAAAACCAA 
AGCAAAGAAG GGGAGAAACA AACCCAATCG GAACCTAAAG AAACTGCATC GGAAAAGGAA 
AGTCCTGGCC AAAAAGAAGA AACCAAAGAA ATTGAAGAAG AACAACAGCC ACGTCTCAAG 
AAACTGCCCT CTACAGCCGA ACTTGAGGAT CGATTTAATG CTTTAGAACG AAAAATGAGC 
GTACAAAAAT CTAGTTCGGC AAAGACAAAG AAAGAGCCAC CCGATGAAGA AAAACCTGCC 
CAAAAAACTG AAGCTCAAAC AAAGGAAACT AAATCTAAAC AAGAATCAAG CCCACAAAAG 
ACTCCAGAAA ACATAGAGAC AAGTGAAAAG AGCACGAAAA TCAGTAAGGA TTCAAATGAG 
GTTAAGGATA AAGCCGATGA GGTAAAACGA AAAGTTCCAC CTTCAACCGA GGAGTTGGAA 
AAGCGTTTCA ATGCTTTGGA GAAACAACTT AGTACAACTA ATTTGGAAGC TGAGCAACCT 
ACTACTTCTA AGGCAAAGGA AATCAAATCA GAATTACCCA AGCAAACTGA AAATGTAAAA 
GCGAACAAGC AAGAAATTCA ATCACAAAAA TCCATCAAGT CTTTCGATGA AAAAGTTAAA 
GTTATTAATA CAAAATTAAC AGCCGATGAG CAGAAAATCC AAGACCACGA CAAATTGGCC 
GAAGAAAAAC GTAAAAGTCT TGATGAGAAA AGTGAAAGAG CCAGTGGTCA GTTAAAAGAA 
ATGTTGCCTG AGAATGAAAC CATTAAATCA AAGACATCTC AACCTGAAGT CGTCCGGGCT 
GATGTTCAAC CCAAAGAACT GGGTAAGCGA AGAGCTTCCG AACCACCATC AACAGAAGAT 
TTGGAGAAAC GCTATGAGAC ACTTAAGCGT CGCATGAGTA GTAAAAATCA TTTTGGTAAT 
CTAACAGAAT CAGCAGATGA GTCCATGTCA AGGCGAGATC AAGAAGTAAT ATCCGAGACT 
TTAGAGACTG GAGAGCCGGA GAGAAAACAA TCACCGCCGA CAACAGAATA TCTGGAAAGT 
AGGTATGAGA AGCTACATGG GGAAAACAAG AAAACAACGC CCAAGAAAGT GGATGTGGCG 
ATTGAGGCGC ATATATCACC ACCACCAGCT CCGCCACCAC CTCCAAAGCC GAATATCCTA 
GCTGATCCGG CTATGCATAA GCAACGAGCT CTCATTGAAG AGCTACAGGC GAAAATTCAA 
GAGCAAGGAC AGTCCACGCC TGGTGAAGAG AATCTCAAGC CCAGCGAGAT AAATCCACAG 
CGCAAACAGA GGCAAGAGCA ACAACGTCTG CTACAACGGC CCACGACAAT GGGAGATGAG 
ACCTCGGAAG CACCTGCAAA GACCGCTTAC TACAGATCGG GAAATTACGA ACCCTGGCAG 
CAGCAGCACC AGCAGAAGCA GCGAATGGTT CGTCGTTTCT CTGATTTGCC ATCAAGAGCC 
GATCTAGAAA ATCGCTTACA ATTCCTGGAG AAGCAATTGT ACAAGAAATT CTACAAGCAG 
CGCTGTGCAA GTGATTCCGA AGTTGCATCG CGGACGATCG ATGACGAAAA GCCCAGCACA 
TCGGCAGCCC GTGTGGCGGA AGTTCAGCTG GAGCAACGTG TCCTGGCCCT AGAGAAGCAA 
TTAAGTGAGA ATAGTCTAAA GCTGCTAGAG GCCATGCGTG TCAACGTGCC CAACGTCGAT 
GGCGGCTCTC CGCGAAGACT AAGCACGGAT ACGATAGATG CCACTGGCAA GGAACTGGTG 
CGGTATACAC AGAACTTTGG TGAGCTGGAC GAGTTGGGTG GAGATGGCTC AAATAAGCCC 
ATTAACATTA GTATCAATAT CAAAATGATG CTCAACAAGG AACAGCAACA ACAACGACAA 
CAACAACAGC AGCAGCAGCA GCAGGAGGAA AAGGCAGACA CTAGGACCAG CACAGAGGAT 
TTAAGGATGC GCTTGGAGCA ATTGGAACAG CAATTGATGG AAGAAAGAGC CAAGAACAAT 
GGTTTTGTGG ATGTCGAAAG TGCTAATGAT GGCGGAGCAG GAGAGGAGGC AAAGCAATTG 
GATAAATCCG AAACTTCAGT ACCGGAAGAA CTAGAGGAAA ATAAAAAGCA AGAGAAGGAC 
AGTCACAATC AAACGGTAAA AAGTGATGAA GCTGAAATGA CAGAAGATCC AATTGTGGCC 
ACCACCGTGG AAACCATTGA GCCGGAGACA GTGAAAGAAA CAAAGACCTT AGAAAACGAG 
GAGATAGCTC AAGCAACCCA AGAGAAAGCT GCAAAAGCTG AGGAGATCGA AGAGACAACC 
AAAGATAAAA TAAATGAATC ATCTGAAGTC ATTCCTAAAG TGGAGGAAAC TAAACCGCCA 
ACAGAAACAG AAACCCACTG CGAAGAGAAA ACTGTAGACT CTGACAAGAT TATTGTCAAT 
GCCAGCTCTA TTGAACCTCC GGTGGATCCC AATAATAAGA CCGTGGTCCT GCTTATGGAT 
AACGAACCTA GAGCTTCGAG AGTGCGCCGT TTGACCCGCG CCAATACCGA AGAACTGGAA 
GGTCTATTCC AGGCTTTAGA AAAACAACTG CAGGATCGCA ATCTAATCAA ATCTGAAGAT 
GGGCGGTTAA TACGAGCAAG TACGGAACAG AAACAAACTG CAGAACAAGT AGAACAAGCT 
CAGGCTATAA GCGATCTAAC TAAGGAAATC GAAGACTTTA CCAGCGGTAA ACCAGACGCA 
AAAAGTGAAG GCGAAACAAA GGAAGATAAG CCAGCAGATG GCGGCGAAGA ACCCCCCGAC 
GATTATGATT GGGGTCCCAA TCCGGTTAAG CATCATTTGA AACGCAAAAC CGTATATTTG 
CCTTCAACCA AAGAGCTAGA GGCTCGTTTT CGGTCACTGG AACGTCAAAT TAAACTACTC 
GAAGATGTGG AGAAAATCGA TGTGGAGCAG CGCTTAAATG AAATTGAACG AAAGATTAAA 
CTGCAGTATT CCTTGTCACA TGAGAAAGAT TTAAATAAAT ATCTAGAGCT CTGCGAAGGC 
AAGGGCTTAG ATGAGGAAGA GGAGCCTATT GTAGATGCAT CTCCTCCTTT AACCAAGGAT 
ACCCAGCGGT CCCGTAGTCC AGCACGTAAA GAAGCTTCAA AGTCGCCATA TACTTCACCA 
TCGCGTAAGG CAGTTGCAAA ATCTCCTCAT ACTTCTCCTG CACGTAAGCC AGCTACAAAA 
TCTCCTTATA CCTCGCCTTC CCGTGCTAGT GACAAAAAGC GGAGCCCTTA TACCTCGCCA 
GTACGTCGAC AGGCGCATCC CGGTGATTTA CCCATCTCCG ATGACTTGGA GTACAAGTAT 
CGTGTGCTTG ATCTGGTACG TTCCAAATCC AAGGAGAACT TGTCGAAGCG TAAAACTGAT 
CCCAATAAAA AGCCGCCCAT TCATCCTCTT GAAATGCTAC TTGATCCGAG TCCCGATGAC 
AGCGAGATAC CCACAACAGG GGAACTGGAG CACCGCATAC GTCTGCTGGA TGAAAAGCTA 
AAGTCGCCCT GCCGCCATAA ATCTCGCTCA CGTTCACCCA CCATCGATGA CATGAAACGA 
AAGAAGCTCT TGGAGGAAAA GCGACCCAAA TCGCCGGTTC ATACATTGGA ACGTTTGGTC 
AGTTCACCTA ACAGACCAGA GCCACCCACT GAGGAGGAAC TAGAACAGCG AATGCGTGCC 
TTGGAAGACG AAAAGCGTTT CGACTTCAAG ACCCAAAAGG ATTACAAGGC ATTCAATCAA 
AAGCTTAAAG ATGTCATCTC ACCTTCCCTA TCTTTCGAAG AGTTCAAGGC GGCCAAATCG 
CGTGAACAGA GTCCACGGCG TCAAGGAGCC ACAACACCCA AATCAGCAAT GCGACGCGAC 
GATACAGATG ATTACCGAGG ACGCGAGGAG ACACCCCATT ATCGGCCCAC TAGTCCTAAG 
GTGATACGCT TTCGAGATGA GGATGAGGAT TATTACAAGG AGAGCCAGCG TCCGAAGTCT 
CGACAGTCCA GCGAACGAAT GACTCTTGCA GATCGCAGCT ACAGCAGCAG CATTGCAGCA 
GCAACGGCCA GCAGCTCCGA GGGTCTCGAT GCCCTGGGTA GTCGCCTAAT GAGAGAAACC 
TCGCCAATTA CCCGCACTGG GACTCATACT GGTGTGCCGT TACGGACTGG AGAGAATATC 
AATGATCGTC TAAGTTCTAT CAAGGATACC ATCAAATCCA TTGACACTCT ATGCGAGGAG 
AAACCGTATC AGAAGGAGAA ATGCCAACGT TATATTGATT CCCTCTTCTC AGATTCATTG 
CATTTCGGTT GTAAGAAGAA TTCCATGGAG GAGCTAACGC ACAGTCGCAG CGAGAGTCGA 
GGACGTACAG CACAACGATC GAGTGACTAT ACGCCGGCCA TTCGAGTCAC CTCAGAGCAT 
CGATCTCTGG GCTCGGCCGA CTCGAAGCGC ACAAGTCCAT TGCGGGCATC CAGTCCGTTG 
CATCATCATC GCTCGCATAG GGATATCAGT AGGGAGTTAT CGCCGCGCAG GCGTCGTGAT 
GCCGAGGATA TGGAGGAGCG CGAAAGTAGT AGGTTGTTGA ATTTCAATTA TGCCATTACA 
TCAAGTTCGA AACATAAGAA AATTATTAAT TCACATTCCA ATGGCTATAT AAATTATTAC 
ATCAAGCAAA GGAACAGAAA ATTAATCAAA ACGATTAGAA AAATATTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_002072004.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002072040.2 Drosophila willistoni uncharacterized protein, transcript variant C (Dwil\GK22633), mRNA.

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CloneID	ODf16565
Clone ID Related Accession (Same CDS sequence)	XM_002072004.2
Accession Version	XM_002072004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform C
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032872). On Dec 21, 2015 this sequence version replaced XM_002072004.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTTTTA GCCTTTCACC ACCGCCGCAG AAAGCTATTA CCGCCAGCGT AACCTCAACC 
AAGAGTAGCA CAAACATTGA CATTAGCTCC AGCTCCAGCA CCATCACCAC CGCGGCTACA 
ACCAGCACCA CCAAAACAAC AACAACCACT TTCTCCACCA CCACCACCAA CAACAAAAAT 
AATATGCTGG GTCATGAGGG GAACAGCTCA AATTCAGCTG CCTCTATTTC TGCATCAGCT 
GACGAAGATG CCGCCTCGGA TTATAATCAA TGGCTGCATG CCATGAAACT GGTGGCTCGT 
CTACCAGGAG GCACACCACC CGAGTTTCGT CGCAAGCTCT GGCTCTCGTT GGCTGACAAA 
TATCTCAAGT CCAAGAATGT TGATTGGACG CAGCAGCGTG AGAAATGCTT TTGCGAGGAA 
TGGCGTGAAG ATGACGAAGA GCTGGGCATA CAAATTGTCA AGGATTTGCA TCGCACTGGT 
TCTAATTTGT GCACTGGTCC CGCGGGCTCC ATTAACCAGG CCAAGCTGAA GCGCATTCTA 
CTCGGCTATG CCCGCTATAA TCCCGAGGTG GGCTATTGTC AGGGCTTCAA TATGCTGGGT 
GCTTTGATTC TACAAGTCAT GGACAAGGAA GAGGAGGAGT CCATGAAGGT CATGATCTAT 
CTGGTGGAGG GTGTACTGCC AACGGGTTAT TTCTATGGTT CAATGGGCGG TCTTCAGGCG 
GATATGGGAG TCTTTCGCGA GCTTATGCAA ACGAAACTGC CGCGTCTGGC CAAGCATTTG 
CAGCGTTTGC AGGGAGGACC CGTTGAGAAT GCCTACGAAC CGCCATTGAC CAATGTCTTC 
ACCATGCAAT GGTTCCTCAC CATGTTCTGC ACCTGCCTAC CCATGTCCTG TGTCCTGCGC 
GTCTGGGATC TCGTTCTCAT CGAAGGCAGT GATGTTCTAT TGCGCACTGC CCTCGTCCTG 
TGGAGTCTAT TGGAAGAACG TGTCCTTAAT GCTCGCACAG CTGATGAGTT CTATGGCAAA 
ATGGGATCTT TCTCGAGTGA ATTGCTCAAT GGTCATCTTA TCGATTCAAA TGGTCTAATC 
GAAAAGGTTG TTAAACTCGG ACCCATTGCC GATTTGCGTC ATTTGAGGGA TAAACATCTA 
TATAACATAG CCCCATTGAG GCATAAACAA GGCATACAAC TCTATTACGA TGACGATGAT 
ACACATTCGG ATGAGGAGCG CATGGCAGTG GCTACCGTGT GGGGTTTAAA TTGGGGTCGC 
CGCGGTTCGG TGGGACCCGG AGCCCCAGCG GGTAGGCAGC CATCTGAACA AAAGGATCGT 
CTGGCCTTGG ATATATCGTT GCTGAAGAAG CAATACGATC GTCTTCGAGA ACGTCAGAAG 
CAGGCCCATG TCATCCTAAC TACTGCATGC TCAACAGCAA CTCGTCAAAC GCCTTCAGCG 
CAAAGCTCGG TGCCGGTTAA TCAGTTACTA CAGGGTCGAC ACGCCATTGT GACCAACAGG 
GGCAAACGGG TGAGTGCTCC CTTGGGAGCC ATACCTCCAG CCCGAAAGCC TTCACTGCCA 
GCTGTATTGC ACGGAAACGG AAAGCCTATG ATGGGGGAGA AGCAGTTGCG ACGAGGAGAA 
ACCCTACTCT GGCGGGACAC GGACACTAGT CGACGGCGAA GAGACAGCCT CACGTGGAAG 
GAGATCAAAG CAGATCGTGC GGCCATGCTA CGCGAAGGAG TTGATTCAAG CTCTCTAAAG 
ACCCAGAAAC TTCGCACGCG GTTGGGCAAA AGTGACAGTT CGTCTTACAG TGAGGATAGC 
GATGGCGATC AGGATGAGAA AGCAGACAAC GGTGGTGCTG GTGGTGGCTC TAGTACGGAC 
ACCAGTCTCT GTGATGACGA TGATCCCAAG TCAAGCGAAA AAAGCCCCAA GCGTAAGGCG 
AAGGTGGCAC GTAAGCTCAA AGAGAAGAGT CAGCATTTGG GGTCTCGGGA GTCCAGCGCA 
GAGCGTCAAC GACCGAAATC CTGGGCACCA GGCACGCATG AGATACCCTT TATGTTGATG 
GGCATGGACA GCGGTGATGA AAAGGAAGCC AAAAAGGAGG AGCAAGACCT GGAGGAAGAC 
AGTGCCACTG AAAGTGGTCG TTTTAGCTAT CCTAAGGAAC TAGATTGGAC TCAGGCGGCA 
GATAAAGTAG CAAGTTGTGA ACTAGAACCA TTGGATATTG ACAAGATACT GCCTAACACT 
GCTATTTATC TAAGCACAAT CAGTCTAAGT CCGCCCAAGA CGGAAGATGT GGGAGATCAG 
GATGTGATAG AGGAACAACC CATTGTGAAG CAGTTTGACG TTAGTGATTC TGGGGTAACA 
AATCAATATT TTGAGCGTGT CAACAGCGTT GAGCGTCCCA ATAGGTTGGA GCTCTCATAC 
TCTCTAAACG AGGAGGAGGA CTCTACAATG TTAAAAGACG ATCGTCAAAG TTCGGATTTT 
GAATTGAAAA TTGAACCCGA ACCTTCCACT TTAAATAAGG TCCCACAAAT TCGGGATGAT 
AACATACCCG GTGAAAACAA AGATGACTAC AAGGAGCTTT TGAGCATGAC GATAGAAGAG 
CATAAGCCAC CAGCAGCTAC AATCACCAGC CAACTGAATG CGAGTCGAAA GCGTCGGGAT 
CCCAGGCGTA AAACGCTAAC CAGATCCTCC ACAATCGAGA TTGAGGAACG TTTTCAGGCC 
CTCGAACGAA CACTGAGCCA AGATCAACCG ATTCATAAGT ACATACCGAG CACTGCAGCT 
CTCGAAGAAC GCTATAATAG TCTAGAAAAA CAGTTGAGTG CCGAGAAACA GCGGAAGGAG 
GAGGATCTGG ATCGTATACC TTCTACAGCT GATCTAGAAT CACGATTTGA TGCCTTAACG 
AAACAAATGA GTTCCAGCGA ATCGAGCAAT TCCAAGGCCC CACTCGATCT TAATAAAGAT 
CAGCAGCAGC CTGGCGAGAA ATCCAAAGAA ACAGAAAGAA AATCAAACAA AAACCAAAGC 
AAAGAAGGGG AGAAACAAAC CCAATCGGAA CCTAAAGAAA CTGCATCGGA AAAGGAAAGT 
CCTGGCCAAA AAGAAGAAAC CAAAGAAATT GAAGAAGAAC AACAGCCACG TCTCAAGAAA 
CTGCCCTCTA CAGCCGAACT TGAGGATCGA TTTAATGCTT TAGAACGAAA AATGAGCGTA 
CAAAAATCTA GTTCGGCAAA GACAAAGAAA GAGCCACCCG ATGAAGAAAA ACCTGCCCAA 
AAAACTGAAG CTCAAACAAA GGAAACTAAA TCTAAACAAG AATCAAGCCC ACAAAAGACT 
CCAGAAAACA TAGAGACAAG TGAAAAGAGC ACGAAAATCA GTAAGGATTC AAATGAGGTT 
AAGGATAAAG CCGATGAGGT AAAACGAAAA GTTCCACCTT CAACCGAGGA GTTGGAAAAG 
CGTTTCAATG CTTTGGAGAA ACAACTTAGT ACAACTAATT TGGAAGCTGA GCAACCTACT 
ACTTCTAAGG CAAAGGAAAT CAAATCAGAA TTACCCAAGC AAACTGAAAA TGTAAAAGCG 
AACAAGCAAG AAATTCAATC ACAAAAATCC ATCAAGTCTT TCGATGAAAA AGTTAAAGTT 
ATTAATACAA AATTAACAGC CGATGAGCAG AAAATCCAAG ACCACGACAA ATTGGCCGAA 
GAAAAACGTA AAAGTCTTGA TGAGAAAAGT GAAAGAGCCA GTGGTCAGTT AAAAGAAATG 
TTGCCTGAGA ATGAAACCAT TAAATCAAAG ACATCTCAAC CTGAAGTCGT CCGGGCTGAT 
GTTCAACCCA AAGAACTGGG TAAGCGAAGA GCTTCCGAAC CACCATCAAC AGAAGATTTG 
GAGAAACGCT ATGAGACACT TAAGCGTCGC ATGAGTAGTA AAAATCATTT TGGTAATCTA 
ACAGAATCAG CAGATGAGTC CATGTCAAGG CGAGATCAAG AAGTAATATC CGAGACTTTA 
GAGACTGGAG AGCCGGAGAG AAAACAATCA CCGCCGACAA CAGAATATCT GGAAAGTAGG 
TATGAGAAGC TACATGGGGA AAACAAGAAA ACAACGCCCA AGAAAGTGGA TGTGGCGATT 
GAGGCGCATA TATCACCACC ACCAGCTCCG CCACCACCTC CAAAGCCGAA TATCCTAGCT 
GATCCGGCTA TGCATAAGCA ACGAGCTCTC ATTGAAGAGC TACAGGCGAA AATTCAAGAG 
CAAGGACAGT CCACGCCTGG TGAAGAGAAT CTCAAGCCCA GCGAGATAAA TCCACAGCGC 
AAACAGAGGC AAGAGCAACA ACGTCTGCTA CAACGGCCCA CGACAATGGG AGATGAGACC 
TCGGAAGCAC CTGCAAAGAC CGCTTACTAC AGATCGGGAA ATTACGAACC CTGGCAGCAG 
CAGCACCAGC AGAAGCAGCG AATGGTTCGT CGTTTCTCTG ATTTGCCATC AAGAGCCGAT 
CTAGAAAATC GCTTACAATT CCTGGAGAAG CAATTGTACA AGAAATTCTA CAAGCAGCGC 
TGTGCAAGTG ATTCCGAAGT TGCATCGCGG ACGATCGATG ACGAAAAGCC CAGCACATCG 
GCAGCCCGTG TGGCGGAAGT TCAGCTGGAG CAACGTGTCC TGGCCCTAGA GAAGCAATTA 
AGTGAGAATA GTCTAAAGCT GCTAGAGGCC ATGCGTGTCA ACGTGCCCAA CGTCGATGGC 
GGCTCTCCGC GAAGACTAAG CACGGATACG ATAGATGCCA CTGGCAAGGA ACTGGTGCGG 
TATACACAGA ACTTTGGTGA GCTGGACGAG TTGGGTGGAG ATGGCTCAAA TAAGCCCATT 
AACATTAGTA TCAATATCAA AATGATGCTC AACAAGGAAC AGCAACAACA ACGACAACAA 
CAACAGCAGC AGCAGCAGCA GGAGGAAAAG GCAGACACTA GGACCAGCAC AGAGGATTTA 
AGGATGCGCT TGGAGCAATT GGAACAGCAA TTGATGGAAG AAAGAGCCAA GAACAATGGT 
TTTGTGGATG TCGAAAGTGC TAATGATGGC GGAGCAGGAG AGGAGGCAAA GCAATTGGAT 
AAATCCGAAA CTTCAGTACC GGAAGAACTA GAGGAAAATA AAAAGCAAGA GAAGGACAGT 
CACAATCAAA CGGTAAAAAG TGATGAAGCT GAAATGACAG AAGATCCAAT TGTGGCCACC 
ACCGTGGAAA CCATTGAGCC GGAGACAGTG AAAGAAACAA AGACCTTAGA AAACGAGGAG 
ATAGCTCAAG CAACCCAAGA GAAAGCTGCA AAAGCTGAGG AGATCGAAGA GACAACCAAA 
GATAAAATAA ATGAATCATC TGAAGTCATT CCTAAAGTGG AGGAAACTAA ACCGCCAACA 
GAAACAGAAA CCCACTGCGA AGAGAAAACT GTAGACTCTG ACAAGATTAT TGTCAATGCC 
AGCTCTATTG AACCTCCGGT GGATCCCAAT AATAAGACCG TGGTCCTGCT TATGGATAAC 
GAACCTAGAG CTTCGAGAGT GCGCCGTTTG ACCCGCGCCA ATACCGAAGA ACTGGAAGGT 
CTATTCCAGG CTTTAGAAAA ACAACTGCAG GATCGCAATC TAATCAAATC TGAAGATGGG 
CGGTTAATAC GAGCAAGTAC GGAACAGAAA CAAACTGCAG AACAAGTAGA ACAAGCTCAG 
GCTATAAGCG ATCTAACTAA GGAAATCGAA GACTTTACCA GCGGTAAACC AGACGCAAAA 
AGTGAAGGCG AAACAAAGGA AGATAAGCCA GCAGATGGCG GCGAAGAACC CCCCGACGAT 
TATGATTGGG GTCCCAATCC GGTTAAGCAT CATTTGAAAC GCAAAACCGT ATATTTGCCT 
TCAACCAAAG AGCTAGAGGC TCGTTTTCGG TCACTGGAAC GTCAAATTAA ACTACTCGAA 
GATGTGGAGA AAATCGATGT GGAGCAGCGC TTAAATGAAA TTGAACGAAA GATTAAACTG 
CAGTATTCCT TGTCACATGA GAAAGATTTA AATAAATATC TAGAGCTCTG CGAAGGCAAG 
GGCTTAGATG AGGAAGAGGA GCCTATTGTA GATGCATCTC CTCCTTTAAC CAAGGATACC 
CAGCGGTCCC GTAGTCCAGC ACGTAAAGAA GCTTCAAAGT CGCCATATAC TTCACCATCG 
CGTAAGGCAG TTGCAAAATC TCCTCATACT TCTCCTGCAC GTAAGCCAGC TACAAAATCT 
CCTTATACCT CGCCTTCCCG TGCTAGTGAC AAAAAGCGGA GCCCTTATAC CTCGCCAGTA 
CGTCGACAGG CGCATCCCGG TGATTTACCC ATCTCCGATG ACTTGGAGTA CAAGTATCGT 
GTGCTTGATC TGGTACGTTC CAAATCCAAG GAGAACTTGT CGAAGCGTAA AACTGATCCC 
AATAAAAAGC CGCCCATTCA TCCTCTTGAA ATGCTACTTG ATCCGAGTCC CGATGACAGC 
GAGATACCCA CAACAGGGGA ACTGGAGCAC CGCATACGTC TGCTGGATGA AAAGCTAAAG 
TCGCCCTGCC GCCATAAATC TCGCTCACGT TCACCCACCA TCGATGACAT GAAACGAAAG 
AAGCTCTTGG AGGAAAAGCG ACCCAAATCG CCGGTTCATA CATTGGAACG TTTGGTCAGT 
TCACCTAACA GACCAGAGCC ACCCACTGAG GAGGAACTAG AACAGCGAAT GCGTGCCTTG 
GAAGACGAAA AGCGTTTCGA CTTCAAGACC CAAAAGGATT ACAAGGCATT CAATCAAAAG 
CTTAAAGATG TCATCTCACC TTCCCTATCT TTCGAAGAGT TCAAGGCGGC CAAATCGCGT 
GAACAGAGTC CACGGCGTCA AGGAGCCACA ACACCCAAAT CAGCAATGCG ACGCGACGAT 
ACAGATGATT ACCGAGGACG CGAGGAGACA CCCCATTATC GGCCCACTAG TCCTAAGGTG 
ATACGCTTTC GAGATGAGGA TGAGGATTAT TACAAGGAGA GCCAGCGTCC GAAGTCTCGA 
CAGTCCAGCG AACGAATGAC TCTTGCAGAT CGCAGCTACA GCAGCAGCAT TGCAGCAGCA 
ACGGCCAGCA GCTCCGAGGG TCTCGATGCC CTGGGTAGTC GCCTAATGAG AGAAACCTCG 
CCAATTACCC GCACTGGGAC TCATACTGGT GTGCCGTTAC GGACTGGAGA GAATATCAAT 
GATCGTCTAA GTTCTATCAA GGATACCATC AAATCCATTG ACACTCTATG CGAGGAGAAA 
CCGTATCAGA AGGAGAAATG CCAACGTTAT ATTGATTCCC TCTTCTCAGA TTCATTGCAT 
TTCGGTTGTA AGAAGAATTC CATGGAGGAG CTAACGCACA GTCGCAGCGA GAGTCGAGGA 
CGTACAGCAC AACGATCGAG TGACTATACG CCGGCCATTC GAGTCACCTC AGAGCATCGA 
TCTCTGGGCT CGGCCGACTC GAAGCGCACA AGTCCATTGC GGGCATCCAG TCCGTTGCAT 
CATCATCGCT CGCATAGGGA TATCAGTAGG GAGTTATCGC CGCGCAGGCG TCGTGATGCC 
GAGGATATGG AGGAGCGCGA AAGTAGTAGG GTAAGACGTG ATAATATGTT GCCAAATTAT 
TTTCTCGATA ATCGTAGCGA TTTAAGTAGC GGCAGTAGTT TAACTAAGTT TCATAAAGTA 
GATAGACAAC TAGAAGAAAC GTGCGCCAAG TACGAAGCTG ACGATCGACG CTCGGCCAGC 
AGAACACCAT TGAGTTCGCC ATATGAGTCC AGAACAACAG CCACACGCTA CAACAACAAC 
AACAACACAG GCACAGGGAC AACAACAACA ACAACCGCCG ATACATTTCA AAGGCCCGTA 
TCGCCCTATC GCCAGCCCTA TGACCCATAT AGACGTAATG TGAATGCCCC ATCCCCGGTT 
CCGGTTTATC AGCCAGCAAA GCTGGAAATT CGTCATACGA CAGTCACATC AACCTTCTAT 
GATCGTTTTC TCACCGAAAA ACAAATTGAG AAGCAAACAC TCTCACGTCC ACCCAGTCGT 
TCGCCTGTAG TCTCACCATC GGCCAGCATA GCCAAAAGCG ATACTGATCT GCCGGGCACA 
GTGAGCAGTG CCTCTGCTTC CGCTTCCGCC TCACAATTCG CTTCCTCCTA CTCCTCCGCC 
GCCTACAAGC CCACAGTAAC CACCGCAATC ATGTCCAGCA GTTATGCTGG TACTGCCACC 
TCACATTTTA CTTCTTCGTT GCCCACAAGC AATTATTCAT ATCTCATGAG CACTTCCACC 
AACGCCACCA CCACCACCAC CTCTTCCTCC TCCACTACTG GTGGTTCGTC TGGGGGATAT 
GTGCCATATA ATTTCAGCAG CTCGTTTAGC AATCGTCATA GTGATCCAAT TGCCAATTCT 
TCTGGCATCT CGAGCGGTAG CACTGGTGTC TATAATCCCA TGATGTCGTT CACTTTGCGT 
GAACCAACAA GTGCCTCGGG CAGTCTCTTG GGTCAATATG CCTACAAAAG TAGTTTCGTC 
TCCAACTACA GTTCGTCATC CAATCAAATG CCCAAGTCAC CATCCACCAG TGATCAGGAC 
AAGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002072040.2
CDS	22..8550
Translation	MRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_002072004.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant C (Dwil\GK22633), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002072004.2

ORF Insert Sequence:

ATGCGTTTTA GCCTTTCACC ACCGCCGCAG AAAGCTATTA CCGCCAGCGT AACCTCAACC 
AAGAGTAGCA CAAACATTGA CATTAGCTCC AGCTCCAGCA CCATCACCAC CGCGGCTACA 
ACCAGCACCA CCAAAACAAC AACAACCACT TTCTCCACCA CCACCACCAA CAACAAAAAT 
AATATGCTGG GTCATGAGGG GAACAGCTCA AATTCAGCTG CCTCTATTTC TGCATCAGCT 
GACGAAGATG CCGCCTCGGA TTATAATCAA TGGCTGCATG CCATGAAACT GGTGGCTCGT 
CTACCAGGAG GCACACCACC CGAGTTTCGT CGCAAGCTCT GGCTCTCGTT GGCTGACAAA 
TATCTCAAGT CCAAGAATGT TGATTGGACG CAGCAGCGTG AGAAATGCTT TTGCGAGGAA 
TGGCGTGAAG ATGACGAAGA GCTGGGCATA CAAATTGTCA AGGATTTGCA TCGCACTGGT 
TCTAATTTGT GCACTGGTCC CGCGGGCTCC ATTAACCAGG CCAAGCTGAA GCGCATTCTA 
CTCGGCTATG CCCGCTATAA TCCCGAGGTG GGCTATTGTC AGGGCTTCAA TATGCTGGGT 
GCTTTGATTC TACAAGTCAT GGACAAGGAA GAGGAGGAGT CCATGAAGGT CATGATCTAT 
CTGGTGGAGG GTGTACTGCC AACGGGTTAT TTCTATGGTT CAATGGGCGG TCTTCAGGCG 
GATATGGGAG TCTTTCGCGA GCTTATGCAA ACGAAACTGC CGCGTCTGGC CAAGCATTTG 
CAGCGTTTGC AGGGAGGACC CGTTGAGAAT GCCTACGAAC CGCCATTGAC CAATGTCTTC 
ACCATGCAAT GGTTCCTCAC CATGTTCTGC ACCTGCCTAC CCATGTCCTG TGTCCTGCGC 
GTCTGGGATC TCGTTCTCAT CGAAGGCAGT GATGTTCTAT TGCGCACTGC CCTCGTCCTG 
TGGAGTCTAT TGGAAGAACG TGTCCTTAAT GCTCGCACAG CTGATGAGTT CTATGGCAAA 
ATGGGATCTT TCTCGAGTGA ATTGCTCAAT GGTCATCTTA TCGATTCAAA TGGTCTAATC 
GAAAAGGTTG TTAAACTCGG ACCCATTGCC GATTTGCGTC ATTTGAGGGA TAAACATCTA 
TATAACATAG CCCCATTGAG GCATAAACAA GGCATACAAC TCTATTACGA TGACGATGAT 
ACACATTCGG ATGAGGAGCG CATGGCAGTG GCTACCGTGT GGGGTTTAAA TTGGGGTCGC 
CGCGGTTCGG TGGGACCCGG AGCCCCAGCG GGTAGGCAGC CATCTGAACA AAAGGATCGT 
CTGGCCTTGG ATATATCGTT GCTGAAGAAG CAATACGATC GTCTTCGAGA ACGTCAGAAG 
CAGGCCCATG TCATCCTAAC TACTGCATGC TCAACAGCAA CTCGTCAAAC GCCTTCAGCG 
CAAAGCTCGG TGCCGGTTAA TCAGTTACTA CAGGGTCGAC ACGCCATTGT GACCAACAGG 
GGCAAACGGG TGAGTGCTCC CTTGGGAGCC ATACCTCCAG CCCGAAAGCC TTCACTGCCA 
GCTGTATTGC ACGGAAACGG AAAGCCTATG ATGGGGGAGA AGCAGTTGCG ACGAGGAGAA 
ACCCTACTCT GGCGGGACAC GGACACTAGT CGACGGCGAA GAGACAGCCT CACGTGGAAG 
GAGATCAAAG CAGATCGTGC GGCCATGCTA CGCGAAGGAG TTGATTCAAG CTCTCTAAAG 
ACCCAGAAAC TTCGCACGCG GTTGGGCAAA AGTGACAGTT CGTCTTACAG TGAGGATAGC 
GATGGCGATC AGGATGAGAA AGCAGACAAC GGTGGTGCTG GTGGTGGCTC TAGTACGGAC 
ACCAGTCTCT GTGATGACGA TGATCCCAAG TCAAGCGAAA AAAGCCCCAA GCGTAAGGCG 
AAGGTGGCAC GTAAGCTCAA AGAGAAGAGT CAGCATTTGG GGTCTCGGGA GTCCAGCGCA 
GAGCGTCAAC GACCGAAATC CTGGGCACCA GGCACGCATG AGATACCCTT TATGTTGATG 
GGCATGGACA GCGGTGATGA AAAGGAAGCC AAAAAGGAGG AGCAAGACCT GGAGGAAGAC 
AGTGCCACTG AAAGTGGTCG TTTTAGCTAT CCTAAGGAAC TAGATTGGAC TCAGGCGGCA 
GATAAAGTAG CAAGTTGTGA ACTAGAACCA TTGGATATTG ACAAGATACT GCCTAACACT 
GCTATTTATC TAAGCACAAT CAGTCTAAGT CCGCCCAAGA CGGAAGATGT GGGAGATCAG 
GATGTGATAG AGGAACAACC CATTGTGAAG CAGTTTGACG TTAGTGATTC TGGGGTAACA 
AATCAATATT TTGAGCGTGT CAACAGCGTT GAGCGTCCCA ATAGGTTGGA GCTCTCATAC 
TCTCTAAACG AGGAGGAGGA CTCTACAATG TTAAAAGACG ATCGTCAAAG TTCGGATTTT 
GAATTGAAAA TTGAACCCGA ACCTTCCACT TTAAATAAGG TCCCACAAAT TCGGGATGAT 
AACATACCCG GTGAAAACAA AGATGACTAC AAGGAGCTTT TGAGCATGAC GATAGAAGAG 
CATAAGCCAC CAGCAGCTAC AATCACCAGC CAACTGAATG CGAGTCGAAA GCGTCGGGAT 
CCCAGGCGTA AAACGCTAAC CAGATCCTCC ACAATCGAGA TTGAGGAACG TTTTCAGGCC 
CTCGAACGAA CACTGAGCCA AGATCAACCG ATTCATAAGT ACATACCGAG CACTGCAGCT 
CTCGAAGAAC GCTATAATAG TCTAGAAAAA CAGTTGAGTG CCGAGAAACA GCGGAAGGAG 
GAGGATCTGG ATCGTATACC TTCTACAGCT GATCTAGAAT CACGATTTGA TGCCTTAACG 
AAACAAATGA GTTCCAGCGA ATCGAGCAAT TCCAAGGCCC CACTCGATCT TAATAAAGAT 
CAGCAGCAGC CTGGCGAGAA ATCCAAAGAA ACAGAAAGAA AATCAAACAA AAACCAAAGC 
AAAGAAGGGG AGAAACAAAC CCAATCGGAA CCTAAAGAAA CTGCATCGGA AAAGGAAAGT 
CCTGGCCAAA AAGAAGAAAC CAAAGAAATT GAAGAAGAAC AACAGCCACG TCTCAAGAAA 
CTGCCCTCTA CAGCCGAACT TGAGGATCGA TTTAATGCTT TAGAACGAAA AATGAGCGTA 
CAAAAATCTA GTTCGGCAAA GACAAAGAAA GAGCCACCCG ATGAAGAAAA ACCTGCCCAA 
AAAACTGAAG CTCAAACAAA GGAAACTAAA TCTAAACAAG AATCAAGCCC ACAAAAGACT 
CCAGAAAACA TAGAGACAAG TGAAAAGAGC ACGAAAATCA GTAAGGATTC AAATGAGGTT 
AAGGATAAAG CCGATGAGGT AAAACGAAAA GTTCCACCTT CAACCGAGGA GTTGGAAAAG 
CGTTTCAATG CTTTGGAGAA ACAACTTAGT ACAACTAATT TGGAAGCTGA GCAACCTACT 
ACTTCTAAGG CAAAGGAAAT CAAATCAGAA TTACCCAAGC AAACTGAAAA TGTAAAAGCG 
AACAAGCAAG AAATTCAATC ACAAAAATCC ATCAAGTCTT TCGATGAAAA AGTTAAAGTT 
ATTAATACAA AATTAACAGC CGATGAGCAG AAAATCCAAG ACCACGACAA ATTGGCCGAA 
GAAAAACGTA AAAGTCTTGA TGAGAAAAGT GAAAGAGCCA GTGGTCAGTT AAAAGAAATG 
TTGCCTGAGA ATGAAACCAT TAAATCAAAG ACATCTCAAC CTGAAGTCGT CCGGGCTGAT 
GTTCAACCCA AAGAACTGGG TAAGCGAAGA GCTTCCGAAC CACCATCAAC AGAAGATTTG 
GAGAAACGCT ATGAGACACT TAAGCGTCGC ATGAGTAGTA AAAATCATTT TGGTAATCTA 
ACAGAATCAG CAGATGAGTC CATGTCAAGG CGAGATCAAG AAGTAATATC CGAGACTTTA 
GAGACTGGAG AGCCGGAGAG AAAACAATCA CCGCCGACAA CAGAATATCT GGAAAGTAGG 
TATGAGAAGC TACATGGGGA AAACAAGAAA ACAACGCCCA AGAAAGTGGA TGTGGCGATT 
GAGGCGCATA TATCACCACC ACCAGCTCCG CCACCACCTC CAAAGCCGAA TATCCTAGCT 
GATCCGGCTA TGCATAAGCA ACGAGCTCTC ATTGAAGAGC TACAGGCGAA AATTCAAGAG 
CAAGGACAGT CCACGCCTGG TGAAGAGAAT CTCAAGCCCA GCGAGATAAA TCCACAGCGC 
AAACAGAGGC AAGAGCAACA ACGTCTGCTA CAACGGCCCA CGACAATGGG AGATGAGACC 
TCGGAAGCAC CTGCAAAGAC CGCTTACTAC AGATCGGGAA ATTACGAACC CTGGCAGCAG 
CAGCACCAGC AGAAGCAGCG AATGGTTCGT CGTTTCTCTG ATTTGCCATC AAGAGCCGAT 
CTAGAAAATC GCTTACAATT CCTGGAGAAG CAATTGTACA AGAAATTCTA CAAGCAGCGC 
TGTGCAAGTG ATTCCGAAGT TGCATCGCGG ACGATCGATG ACGAAAAGCC CAGCACATCG 
GCAGCCCGTG TGGCGGAAGT TCAGCTGGAG CAACGTGTCC TGGCCCTAGA GAAGCAATTA 
AGTGAGAATA GTCTAAAGCT GCTAGAGGCC ATGCGTGTCA ACGTGCCCAA CGTCGATGGC 
GGCTCTCCGC GAAGACTAAG CACGGATACG ATAGATGCCA CTGGCAAGGA ACTGGTGCGG 
TATACACAGA ACTTTGGTGA GCTGGACGAG TTGGGTGGAG ATGGCTCAAA TAAGCCCATT 
AACATTAGTA TCAATATCAA AATGATGCTC AACAAGGAAC AGCAACAACA ACGACAACAA 
CAACAGCAGC AGCAGCAGCA GGAGGAAAAG GCAGACACTA GGACCAGCAC AGAGGATTTA 
AGGATGCGCT TGGAGCAATT GGAACAGCAA TTGATGGAAG AAAGAGCCAA GAACAATGGT 
TTTGTGGATG TCGAAAGTGC TAATGATGGC GGAGCAGGAG AGGAGGCAAA GCAATTGGAT 
AAATCCGAAA CTTCAGTACC GGAAGAACTA GAGGAAAATA AAAAGCAAGA GAAGGACAGT 
CACAATCAAA CGGTAAAAAG TGATGAAGCT GAAATGACAG AAGATCCAAT TGTGGCCACC 
ACCGTGGAAA CCATTGAGCC GGAGACAGTG AAAGAAACAA AGACCTTAGA AAACGAGGAG 
ATAGCTCAAG CAACCCAAGA GAAAGCTGCA AAAGCTGAGG AGATCGAAGA GACAACCAAA 
GATAAAATAA ATGAATCATC TGAAGTCATT CCTAAAGTGG AGGAAACTAA ACCGCCAACA 
GAAACAGAAA CCCACTGCGA AGAGAAAACT GTAGACTCTG ACAAGATTAT TGTCAATGCC 
AGCTCTATTG AACCTCCGGT GGATCCCAAT AATAAGACCG TGGTCCTGCT TATGGATAAC 
GAACCTAGAG CTTCGAGAGT GCGCCGTTTG ACCCGCGCCA ATACCGAAGA ACTGGAAGGT 
CTATTCCAGG CTTTAGAAAA ACAACTGCAG GATCGCAATC TAATCAAATC TGAAGATGGG 
CGGTTAATAC GAGCAAGTAC GGAACAGAAA CAAACTGCAG AACAAGTAGA ACAAGCTCAG 
GCTATAAGCG ATCTAACTAA GGAAATCGAA GACTTTACCA GCGGTAAACC AGACGCAAAA 
AGTGAAGGCG AAACAAAGGA AGATAAGCCA GCAGATGGCG GCGAAGAACC CCCCGACGAT 
TATGATTGGG GTCCCAATCC GGTTAAGCAT CATTTGAAAC GCAAAACCGT ATATTTGCCT 
TCAACCAAAG AGCTAGAGGC TCGTTTTCGG TCACTGGAAC GTCAAATTAA ACTACTCGAA 
GATGTGGAGA AAATCGATGT GGAGCAGCGC TTAAATGAAA TTGAACGAAA GATTAAACTG 
CAGTATTCCT TGTCACATGA GAAAGATTTA AATAAATATC TAGAGCTCTG CGAAGGCAAG 
GGCTTAGATG AGGAAGAGGA GCCTATTGTA GATGCATCTC CTCCTTTAAC CAAGGATACC 
CAGCGGTCCC GTAGTCCAGC ACGTAAAGAA GCTTCAAAGT CGCCATATAC TTCACCATCG 
CGTAAGGCAG TTGCAAAATC TCCTCATACT TCTCCTGCAC GTAAGCCAGC TACAAAATCT 
CCTTATACCT CGCCTTCCCG TGCTAGTGAC AAAAAGCGGA GCCCTTATAC CTCGCCAGTA 
CGTCGACAGG CGCATCCCGG TGATTTACCC ATCTCCGATG ACTTGGAGTA CAAGTATCGT 
GTGCTTGATC TGGTACGTTC CAAATCCAAG GAGAACTTGT CGAAGCGTAA AACTGATCCC 
AATAAAAAGC CGCCCATTCA TCCTCTTGAA ATGCTACTTG ATCCGAGTCC CGATGACAGC 
GAGATACCCA CAACAGGGGA ACTGGAGCAC CGCATACGTC TGCTGGATGA AAAGCTAAAG 
TCGCCCTGCC GCCATAAATC TCGCTCACGT TCACCCACCA TCGATGACAT GAAACGAAAG 
AAGCTCTTGG AGGAAAAGCG ACCCAAATCG CCGGTTCATA CATTGGAACG TTTGGTCAGT 
TCACCTAACA GACCAGAGCC ACCCACTGAG GAGGAACTAG AACAGCGAAT GCGTGCCTTG 
GAAGACGAAA AGCGTTTCGA CTTCAAGACC CAAAAGGATT ACAAGGCATT CAATCAAAAG 
CTTAAAGATG TCATCTCACC TTCCCTATCT TTCGAAGAGT TCAAGGCGGC CAAATCGCGT 
GAACAGAGTC CACGGCGTCA AGGAGCCACA ACACCCAAAT CAGCAATGCG ACGCGACGAT 
ACAGATGATT ACCGAGGACG CGAGGAGACA CCCCATTATC GGCCCACTAG TCCTAAGGTG 
ATACGCTTTC GAGATGAGGA TGAGGATTAT TACAAGGAGA GCCAGCGTCC GAAGTCTCGA 
CAGTCCAGCG AACGAATGAC TCTTGCAGAT CGCAGCTACA GCAGCAGCAT TGCAGCAGCA 
ACGGCCAGCA GCTCCGAGGG TCTCGATGCC CTGGGTAGTC GCCTAATGAG AGAAACCTCG 
CCAATTACCC GCACTGGGAC TCATACTGGT GTGCCGTTAC GGACTGGAGA GAATATCAAT 
GATCGTCTAA GTTCTATCAA GGATACCATC AAATCCATTG ACACTCTATG CGAGGAGAAA 
CCGTATCAGA AGGAGAAATG CCAACGTTAT ATTGATTCCC TCTTCTCAGA TTCATTGCAT 
TTCGGTTGTA AGAAGAATTC CATGGAGGAG CTAACGCACA GTCGCAGCGA GAGTCGAGGA 
CGTACAGCAC AACGATCGAG TGACTATACG CCGGCCATTC GAGTCACCTC AGAGCATCGA 
TCTCTGGGCT CGGCCGACTC GAAGCGCACA AGTCCATTGC GGGCATCCAG TCCGTTGCAT 
CATCATCGCT CGCATAGGGA TATCAGTAGG GAGTTATCGC CGCGCAGGCG TCGTGATGCC 
GAGGATATGG AGGAGCGCGA AAGTAGTAGG GTAAGACGTG ATAATATGTT GCCAAATTAT 
TTTCTCGATA ATCGTAGCGA TTTAAGTAGC GGCAGTAGTT TAACTAAGTT TCATAAAGTA 
GATAGACAAC TAGAAGAAAC GTGCGCCAAG TACGAAGCTG ACGATCGACG CTCGGCCAGC 
AGAACACCAT TGAGTTCGCC ATATGAGTCC AGAACAACAG CCACACGCTA CAACAACAAC 
AACAACACAG GCACAGGGAC AACAACAACA ACAACCGCCG ATACATTTCA AAGGCCCGTA 
TCGCCCTATC GCCAGCCCTA TGACCCATAT AGACGTAATG TGAATGCCCC ATCCCCGGTT 
CCGGTTTATC AGCCAGCAAA GCTGGAAATT CGTCATACGA CAGTCACATC AACCTTCTAT 
GATCGTTTTC TCACCGAAAA ACAAATTGAG AAGCAAACAC TCTCACGTCC ACCCAGTCGT 
TCGCCTGTAG TCTCACCATC GGCCAGCATA GCCAAAAGCG ATACTGATCT GCCGGGCACA 
GTGAGCAGTG CCTCTGCTTC CGCTTCCGCC TCACAATTCG CTTCCTCCTA CTCCTCCGCC 
GCCTACAAGC CCACAGTAAC CACCGCAATC ATGTCCAGCA GTTATGCTGG TACTGCCACC 
TCACATTTTA CTTCTTCGTT GCCCACAAGC AATTATTCAT ATCTCATGAG CACTTCCACC 
AACGCCACCA CCACCACCAC CTCTTCCTCC TCCACTACTG GTGGTTCGTC TGGGGGATAT 
GTGCCATATA ATTTCAGCAG CTCGTTTAGC AATCGTCATA GTGATCCAAT TGCCAATTCT 
TCTGGCATCT CGAGCGGTAG CACTGGTGTC TATAATCCCA TGATGTCGTT CACTTTGCGT 
GAACCAACAA GTGCCTCGGG CAGTCTCTTG GGTCAATATG CCTACAAAAG TAGTTTCGTC 
TCCAACTACA GTTCGTCATC CAATCAAATG CCCAAGTCAC CATCCACCAG TGATCAGGAC 
AAGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_015177070.1 ) cDNA ORF clone, Drosophila willistoni -> XP_015032556.1 Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK22633), mRNA.

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CloneID	ODf16725
Clone ID Related Accession (Same CDS sequence)	XM_015177070.1
Accession Version	XM_015177070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform B
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032872). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATG CACAGCCTAA AATGCGTTTT AGCCTTTCAC CACCGCCGCA GAAAGCTATT 
ACCGCCAGCG TAACCTCAAC CAAGAGTAGC ACAAACATTG ACATTAGCTC CAGCTCCAGC 
ACCATCACCA CCGCGGCTAC AACCAGCACC ACCAAAACAA CAACAACCAC TTTCTCCACC 
ACCACCACCA ACAACAAAAA TAATATGCTG GGTCATGAGG GGAACAGCTC AAATTCAGCT 
GCCTCTATTT CTGCATCAGC TGACGAAGAT GCCGCCTCGG ATTATAATCA ATGGCTGCAT 
GCCATGAAAC TGGTGGCTCG TCTACCAGGA GGCACACCAC CCGAGTTTCG TCGCAAGCTC 
TGGCTCTCGT TGGCTGACAA ATATCTCAAG TCCAAGAATG TTGATTGGAC GCAGCAGCGT 
GAGAAATGCT TTTGCGAGGA ATGGCGTGAA GATGACGAAG AGCTGGGCAT ACAAATTGTC 
AAGGATTTGC ATCGCACTGG TTCTAATTTG TGCACTGGTC CCGCGGGCTC CATTAACCAG 
GCCAAGCTGA AGCGCATTCT ACTCGGCTAT GCCCGCTATA ATCCCGAGGT GGGCTATTGT 
CAGGGCTTCA ATATGCTGGG TGCTTTGATT CTACAAGTCA TGGACAAGGA AGAGGAGGAG 
TCCATGAAGG TCATGATCTA TCTGGTGGAG GGTGTACTGC CAACGGGTTA TTTCTATGGT 
TCAATGGGCG GTCTTCAGGC GGATATGGGA GTCTTTCGCG AGCTTATGCA AACGAAACTG 
CCGCGTCTGG CCAAGCATTT GCAGCGTTTG CAGGGAGGAC CCGTTGAGAA TGCCTACGAA 
CCGCCATTGA CCAATGTCTT CACCATGCAA TGGTTCCTCA CCATGTTCTG CACCTGCCTA 
CCCATGTCCT GTGTCCTGCG CGTCTGGGAT CTCGTTCTCA TCGAAGGCAG TGATGTTCTA 
TTGCGCACTG CCCTCGTCCT GTGGAGTCTA TTGGAAGAAC GTGTCCTTAA TGCTCGCACA 
GCTGATGAGT TCTATGGCAA AATGGGATCT TTCTCGAGTG AATTGCTCAA TGGTCATCTT 
ATCGATTCAA ATGGTCTAAT CGAAAAGGTT GTTAAACTCG GACCCATTGC CGATTTGCGT 
CATTTGAGGG ATAAACATCT ATATAACATA GCCCCATTGA GGCATAAACA AGGCATACAA 
CTCTATTACG ATGACGATGA TACACATTCG GATGAGGAGC GCATGGCAGT GGCTACCGTG 
TGGGGTTTAA ATTGGGGTCG CCGCGGTTCG GTGGGACCCG GAGCCCCAGC GGGTAGGCAG 
CCATCTGAAC AAAAGGATCG TCTGGCCTTG GATATATCGT TGCTGAAGAA GCAATACGAT 
CGTCTTCGAG AACGTCAGAA GCAGGCCCAT GTCATCCTAA CTACTGCATG CTCAACAGCA 
ACTCGTCAAA CGCCTTCAGC GCAAAGCTCG GTGCCGGTTA ATCAGTTACT ACAGGGTCGA 
CACGCCATTG TGACCAACAG GGGCAAACGG GTGAGTGCTC CCTTGGGAGC CATACCTCCA 
GCCCGAAAGC CTTCACTGCC AGCTGTATTG CACGGAAACG GAAAGCCTAT GATGGGGGAG 
AAGCAGTTGC GACGAGGAGA AACCCTACTC TGGCGGGACA CGGACACTAG TCGACGGCGA 
AGAGACAGCC TCACGTGGAA GGAGATCAAA GCAGATCGTG CGGCCATGCT ACGCGAAGGA 
GTTGATTCAA GCTCTCTAAA GACCCAGAAA CTTCGCACGC GGTTGGGCAA AAGTGACAGT 
TCGTCTTACA GTGAGGATAG CGATGGCGAT CAGGATGAGA AAGCAGACAA CGGTGGTGCT 
GGTGGTGGCT CTAGTACGGA CACCAGTCTC TGTGATGACG ATGATCCCAA GTCAAGCGAA 
AAAAGCCCCA AGCGTAAGGC GAAGGTGGCA CGTAAGCTCA AAGAGAAGAG TCAGCATTTG 
GGGTCTCGGG AGTCCAGCGC AGAGCGTCAA CGACCGAAAT CCTGGGCACC AGGCACGCAT 
GAGATACCCT TTATGTTGAT GGGCATGGAC AGCGGTGATG AAAAGGAAGC CAAAAAGGAG 
GAGCAAGACC TGGAGGAAGA CAGTGCCACT GAAAGTGGTC GTTTTAGCTA TCCTAAGGAA 
CTAGATTGGA CTCAGGCGGC AGATAAAGTA GCAAGTTGTG AACTAGAACC ATTGGATATT 
GACAAGATAC TGCCTAACAC TGCTATTTAT CTAAGCACAA TCAGTCTAAG TCCGCCCAAG 
ACGGAAGATG TGGGAGATCA GGATGTGATA GAGGAACAAC CCATTGTGAA GCAGTTTGAC 
GTTAGTGATT CTGGGGTAAC AAATCAATAT TTTGAGCGTG TCAACAGCGT TGAGCGTCCC 
AATAGGTTGG AGCTCTCATA CTCTCTAAAC GAGGAGGAGG ACTCTACAAT GTTAAAAGAC 
GATCGTCAAA GTTCGGATTT TGAATTGAAA ATTGAACCCG AACCTTCCAC TTTAAATAAG 
GTCCCACAAA TTCGGGATGA TAACATACCC GGTGAAAACA AAGATGACTA CAAGGAGCTT 
TTGAGCATGA CGATAGAAGA GCATAAGCCA CCAGCAGCTA CAATCACCAG CCAACTGAAT 
GCGAGTCGAA AGCGTCGGGA TCCCAGGCGT AAAACGCTAA CCAGATCCTC CACAATCGAG 
ATTGAGGAAC GTTTTCAGGC CCTCGAACGA ACACTGAGCC AAGATCAACC GATTCATAAG 
TACATACCGA GCACTGCAGC TCTCGAAGAA CGCTATAATA GTCTAGAAAA ACAGTTGAGT 
GCCGAGAAAC AGCGGAAGGA GGAGGATCTG GATCGTATAC CTTCTACAGC TGATCTAGAA 
TCACGATTTG ATGCCTTAAC GAAACAAATG AGTTCCAGCG AATCGAGCAA TTCCAAGGCC 
CCACTCGATC TTAATAAAGA TCAGCAGCAG CCTGGCGAGA AATCCAAAGA AACAGAAAGA 
AAATCAAACA AAAACCAAAG CAAAGAAGGG GAGAAACAAA CCCAATCGGA ACCTAAAGAA 
ACTGCATCGG AAAAGGAAAG TCCTGGCCAA AAAGAAGAAA CCAAAGAAAT TGAAGAAGAA 
CAACAGCCAC GTCTCAAGAA ACTGCCCTCT ACAGCCGAAC TTGAGGATCG ATTTAATGCT 
TTAGAACGAA AAATGAGCGT ACAAAAATCT AGTTCGGCAA AGACAAAGAA AGAGCCACCC 
GATGAAGAAA AACCTGCCCA AAAAACTGAA GCTCAAACAA AGGAAACTAA ATCTAAACAA 
GAATCAAGCC CACAAAAGAC TCCAGAAAAC ATAGAGACAA GTGAAAAGAG CACGAAAATC 
AGTAAGGATT CAAATGAGGT TAAGGATAAA GCCGATGAGG TAAAACGAAA AGTTCCACCT 
TCAACCGAGG AGTTGGAAAA GCGTTTCAAT GCTTTGGAGA AACAACTTAG TACAACTAAT 
TTGGAAGCTG AGCAACCTAC TACTTCTAAG GCAAAGGAAA TCAAATCAGA ATTACCCAAG 
CAAACTGAAA ATGTAAAAGC GAACAAGCAA GAAATTCAAT CACAAAAATC CATCAAGTCT 
TTCGATGAAA AAGTTAAAGT TATTAATACA AAATTAACAG CCGATGAGCA GAAAATCCAA 
GACCACGACA AATTGGCCGA AGAAAAACGT AAAAGTCTTG ATGAGAAAAG TGAAAGAGCC 
AGTGGTCAGT TAAAAGAAAT GTTGCCTGAG AATGAAACCA TTAAATCAAA GACATCTCAA 
CCTGAAGTCG TCCGGGCTGA TGTTCAACCC AAAGAACTGG GTAAGCGAAG AGCTTCCGAA 
CCACCATCAA CAGAAGATTT GGAGAAACGC TATGAGACAC TTAAGCGTCG CATGAGTAGT 
AAAAATCATT TTGGTAATCT AACAGAATCA GCAGATGAGT CCATGTCAAG GCGAGATCAA 
GAAGTAATAT CCGAGACTTT AGAGACTGGA GAGCCGGAGA GAAAACAATC ACCGCCGACA 
ACAGAATATC TGGAAAGTAG GTATGAGAAG CTACATGGGG AAAACAAGAA AACAACGCCC 
AAGAAAGTGG ATGTGGCGAT TGAGGCGCAT ATATCACCAC CACCAGCTCC GCCACCACCT 
CCAAAGCCGA ATATCCTAGC TGATCCGGCT ATGCATAAGC AACGAGCTCT CATTGAAGAG 
CTACAGGCGA AAATTCAAGA GCAAGGACAG TCCACGCCTG GTGAAGAGAA TCTCAAGCCC 
AGCGAGATAA ATCCACAGCG CAAACAGAGG CAAGAGCAAC AACGTCTGCT ACAACGGCCC 
ACGACAATGG GAGATGAGAC CTCGGAAGCA CCTGCAAAGA CCGCTTACTA CAGATCGGGA 
AATTACGAAC CCTGGCAGCA GCAGCACCAG CAGAAGCAGC GAATGGTTCG TCGTTTCTCT 
GATTTGCCAT CAAGAGCCGA TCTAGAAAAT CGCTTACAAT TCCTGGAGAA GCAATTGTAC 
AAGAAATTCT ACAAGCAGCG CTGTGCAAGT GATTCCGAAG TTGCATCGCG GACGATCGAT 
GACGAAAAGC CCAGCACATC GGCAGCCCGT GTGGCGGAAG TTCAGCTGGA GCAACGTGTC 
CTGGCCCTAG AGAAGCAATT AAGTGAGAAT AGTCTAAAGC TGCTAGAGGC CATGCGTGTC 
AACGTGCCCA ACGTCGATGG CGGCTCTCCG CGAAGACTAA GCACGGATAC GATAGATGCC 
ACTGGCAAGG AACTGGTGCG GTATACACAG AACTTTGGTG AGCTGGACGA GTTGGGTGGA 
GATGGCTCAA ATAAGCCCAT TAACATTAGT ATCAATATCA AAATGATGCT CAACAAGGAA 
CAGCAACAAC AACGACAACA ACAACAGCAG CAGCAGCAGC AGGAGGAAAA GGCAGACACT 
AGGACCAGCA CAGAGGATTT AAGGATGCGC TTGGAGCAAT TGGAACAGCA ATTGATGGAA 
GAAAGAGCCA AGAACAATGG TTTTGTGGAT GTCGAAAGTG CTAATGATGG CGGAGCAGGA 
GAGGAGGCAA AGCAATTGGA TAAATCCGAA ACTTCAGTAC CGGAAGAACT AGAGGAAAAT 
AAAAAGCAAG AGAAGGACAG TCACAATCAA ACGGTAAAAA GTGATGAAGC TGAAATGACA 
GAAGATCCAA TTGTGGCCAC CACCGTGGAA ACCATTGAGC CGGAGACAGT GAAAGAAACA 
AAGACCTTAG AAAACGAGGA GATAGCTCAA GCAACCCAAG AGAAAGCTGC AAAAGCTGAG 
GAGATCGAAG AGACAACCAA AGATAAAATA AATGAATCAT CTGAAGTCAT TCCTAAAGTG 
GAGGAAACTA AACCGCCAAC AGAAACAGAA ACCCACTGCG AAGAGAAAAC TGTAGACTCT 
GACAAGATTA TTGTCAATGC CAGCTCTATT GAACCTCCGG TGGATCCCAA TAATAAGACC 
GTGGTCCTGC TTATGGATAA CGAACCTAGA GCTTCGAGAG TGCGCCGTTT GACCCGCGCC 
AATACCGAAG AACTGGAAGG TCTATTCCAG GCTTTAGAAA AACAACTGCA GGATCGCAAT 
CTAATCAAAT CTGAAGATGG GCGGTTAATA CGAGCAAGTA CGGAACAGAA ACAAACTGCA 
GAACAAGTAG AACAAGCTCA GGCTATAAGC GATCTAACTA AGGAAATCGA AGACTTTACC 
AGCGGTAAAC CAGACGCAAA AAGTGAAGGC GAAACAAAGG AAGATAAGCC AGCAGATGGC 
GGCGAAGAAC CCCCCGACGA TTATGATTGG GGTCCCAATC CGGTTAAGCA TCATTTGAAA 
CGCAAAACCG TATATTTGCC TTCAACCAAA GAGCTAGAGG CTCGTTTTCG GTCACTGGAA 
CGTCAAATTA AACTACTCGA AGATGTGGAG AAAATCGATG TGGAGCAGCG CTTAAATGAA 
ATTGAACGAA AGATTAAACT GCAGTATTCC TTGTCACATG AGAAAGATTT AAATAAATAT 
CTAGAGCTCT GCGAAGGCAA GGGCTTAGAT GAGGAAGAGG AGCCTATTGT AGATGCATCT 
CCTCCTTTAA CCAAGGATAC CCAGCGGTCC CGTAGTCCAG CACGTAAAGA AGCTTCAAAG 
TCGCCATATA CTTCACCATC GCGTAAGGCA GTTGCAAAAT CTCCTCATAC TTCTCCTGCA 
CGTAAGCCAG CTACAAAATC TCCTTATACC TCGCCTTCCC GTGCTAGTGA CAAAAAGCGG 
AGCCCTTATA CCTCGCCAGT ACGTCGACAG GCGCATCCCG GTGATTTACC CATCTCCGAT 
GACTTGGAGT ACAAGTATCG TGTGCTTGAT CTGGTACGTT CCAAATCCAA GGAGAACTTG 
TCGAAGCGTA AAACTGATCC CAATAAAAAG CCGCCCATTC ATCCTCTTGA AATGCTACTT 
GATCCGAGTC CCGATGACAG CGAGATACCC ACAACAGGGG AACTGGAGCA CCGCATACGT 
CTGCTGGATG AAAAGCTAAA GTCGCCCTGC CGCCATAAAT CTCGCTCACG TTCACCCACC 
ATCGATGACA TGAAACGAAA GAAGCTCTTG GAGGAAAAGC GACCCAAATC GCCGGTTCAT 
ACATTGGAAC GTTTGGTCAG TTCACCTAAC AGACCAGAGC CACCCACTGA GGAGGAACTA 
GAACAGCGAA TGCGTGCCTT GGAAGACGAA AAGCGTTTCG ACTTCAAGAC CCAAAAGGAT 
TACAAGGCAT TCAATCAAAA GCTTAAAGAT GTCATCTCAC CTTCCCTATC TTTCGAAGAG 
TTCAAGGCGG CCAAATCGCG TGAACAGAGT CCACGGCGTC AAGGAGCCAC AACACCCAAA 
TCAGCAATGC GACGCGACGA TACAGATGAT TACCGAGGAC GCGAGGAGAC ACCCCATTAT 
CGGCCCACTA GTCCTAAGGT GATACGCTTT CGAGATGAGG ATGAGGATTA TTACAAGGAG 
AGCCAGCGTC CGAAGTCTCG ACAGTCCAGC GAACGAATGA CTCTTGCAGA TCGCAGCTAC 
AGCAGCAGCA TTGCAGCAGC AACGGCCAGC AGCTCCGAGG GTCTCGATGC CCTGGGTAGT 
CGCCTAATGA GAGAAACCTC GCCAATTACC CGCACTGGGA CTCATACTGG TGTGCCGTTA 
CGGACTGGAG AGAATATCAA TGATCGTCTA AGTTCTATCA AGGATACCAT CAAATCCATT 
GACACTCTAT GCGAGGAGAA ACCGTATCAG AAGGAGAAAT GCCAACGTTA TATTGATTCC 
CTCTTCTCAG ATTCATTGCA TTTCGGTTGT AAGAAGAATT CCATGGAGGA GCTAACGCAC 
AGTCGCAGCG AGAGTCGAGG ACGTACAGCA CAACGATCGA GTGACTATAC GCCGGCCATT 
CGAGTCACCT CAGAGCATCG ATCTCTGGGC TCGGCCGACT CGAAGCGCAC AAGTCCATTG 
CGGGCATCCA GTCCGTTGCA TCATCATCGC TCGCATAGGG ATATCAGTAG GGAGTTATCG 
CCGCGCAGGC GTCGTGATGC CGAGGATATG GAGGAGCGCG AAAGTAGTAG GGTAAGACGT 
GATAATATGT TGCCAAATTA TTTTCTCGAT AATCGTAGCG ATTTAAGTAG CGGCAGTAGT 
TTAACTAAGT TTCATAAAGT AGATAGACAA CTAGAAGAAA CGTGCGCCAA GTACGAAGCT 
GACGATCGAC GCTCGGCCAG CAGAACACCA TTGAGTTCGC CATATGAGTC CAGAACAACA 
GCCACACGCT ACAACAACAA CAACAACACA GGCACAGGGA CAACAACAAC AACAACCGCC 
GATACATTTC AAAGGCCCGT ATCGCCCTAT CGCCAGCCCT ATGACCCATA TAGACGTAAT 
GTGAATGCCC CATCCCCGGT TCCGGTTTAT CAGCCAGCAA AGCTGGAAAT TCGTCATACG 
ACAGTCACAT CAACCTTCTA TGATCGTTTT CTCACCGAAA AACAAATTGA GAAGCAAACA 
CTCTCACGTC CACCCAGTCG TTCGCCTGTA GTCTCACCAT CGGCCAGCAT AGCCAAAAGC 
GATACTGATC TGCCGGGCAC AGTGAGCAGT GCCTCTGCTT CCGCTTCCGC CTCACAATTC 
GCTTCCTCCT ACTCCTCCGC CGCCTACAAG CCCACAGTAA CCACCGCAAT CATGTCCAGC 
AGTTATGCTG GTACTGCCAC CTCACATTTT ACTTCTTCGT TGCCCACAAG CAATTATTCA 
TATCTCATGA GCACTTCCAC CAACGCCACC ACCACCACCA CCTCTTCCTC CTCCACTACT 
GGTGGTTCGT CTGGGGGATA TGTGCCATAT AATTTCAGCA GCTCGTTTAG CAATCGTCAT 
AGTGATCCAA TTGCCAATTC TTCTGGCATC TCGAGCGGTA GCACTGGTGT CTATAATCCC 
ATGATGTCGT TCACTTTGCG TGAACCAACA AGTGCCTCGG GCAGTCTCTT GGGTCAATAT 
GCCTACAAAA GTAGTTTCGT CTCCAACTAC AGTTCGTCAT CCAATCAAAT GCCCAAGTCA 
CCATCCACCA GTGATCAGGA CAAGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015032556.1
CDS	5..8554
Translation	MDDAQPKMRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_015177070.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK22633), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015177070.1

ORF Insert Sequence:

ATGGACGATG CACAGCCTAA AATGCGTTTT AGCCTTTCAC CACCGCCGCA GAAAGCTATT 
ACCGCCAGCG TAACCTCAAC CAAGAGTAGC ACAAACATTG ACATTAGCTC CAGCTCCAGC 
ACCATCACCA CCGCGGCTAC AACCAGCACC ACCAAAACAA CAACAACCAC TTTCTCCACC 
ACCACCACCA ACAACAAAAA TAATATGCTG GGTCATGAGG GGAACAGCTC AAATTCAGCT 
GCCTCTATTT CTGCATCAGC TGACGAAGAT GCCGCCTCGG ATTATAATCA ATGGCTGCAT 
GCCATGAAAC TGGTGGCTCG TCTACCAGGA GGCACACCAC CCGAGTTTCG TCGCAAGCTC 
TGGCTCTCGT TGGCTGACAA ATATCTCAAG TCCAAGAATG TTGATTGGAC GCAGCAGCGT 
GAGAAATGCT TTTGCGAGGA ATGGCGTGAA GATGACGAAG AGCTGGGCAT ACAAATTGTC 
AAGGATTTGC ATCGCACTGG TTCTAATTTG TGCACTGGTC CCGCGGGCTC CATTAACCAG 
GCCAAGCTGA AGCGCATTCT ACTCGGCTAT GCCCGCTATA ATCCCGAGGT GGGCTATTGT 
CAGGGCTTCA ATATGCTGGG TGCTTTGATT CTACAAGTCA TGGACAAGGA AGAGGAGGAG 
TCCATGAAGG TCATGATCTA TCTGGTGGAG GGTGTACTGC CAACGGGTTA TTTCTATGGT 
TCAATGGGCG GTCTTCAGGC GGATATGGGA GTCTTTCGCG AGCTTATGCA AACGAAACTG 
CCGCGTCTGG CCAAGCATTT GCAGCGTTTG CAGGGAGGAC CCGTTGAGAA TGCCTACGAA 
CCGCCATTGA CCAATGTCTT CACCATGCAA TGGTTCCTCA CCATGTTCTG CACCTGCCTA 
CCCATGTCCT GTGTCCTGCG CGTCTGGGAT CTCGTTCTCA TCGAAGGCAG TGATGTTCTA 
TTGCGCACTG CCCTCGTCCT GTGGAGTCTA TTGGAAGAAC GTGTCCTTAA TGCTCGCACA 
GCTGATGAGT TCTATGGCAA AATGGGATCT TTCTCGAGTG AATTGCTCAA TGGTCATCTT 
ATCGATTCAA ATGGTCTAAT CGAAAAGGTT GTTAAACTCG GACCCATTGC CGATTTGCGT 
CATTTGAGGG ATAAACATCT ATATAACATA GCCCCATTGA GGCATAAACA AGGCATACAA 
CTCTATTACG ATGACGATGA TACACATTCG GATGAGGAGC GCATGGCAGT GGCTACCGTG 
TGGGGTTTAA ATTGGGGTCG CCGCGGTTCG GTGGGACCCG GAGCCCCAGC GGGTAGGCAG 
CCATCTGAAC AAAAGGATCG TCTGGCCTTG GATATATCGT TGCTGAAGAA GCAATACGAT 
CGTCTTCGAG AACGTCAGAA GCAGGCCCAT GTCATCCTAA CTACTGCATG CTCAACAGCA 
ACTCGTCAAA CGCCTTCAGC GCAAAGCTCG GTGCCGGTTA ATCAGTTACT ACAGGGTCGA 
CACGCCATTG TGACCAACAG GGGCAAACGG GTGAGTGCTC CCTTGGGAGC CATACCTCCA 
GCCCGAAAGC CTTCACTGCC AGCTGTATTG CACGGAAACG GAAAGCCTAT GATGGGGGAG 
AAGCAGTTGC GACGAGGAGA AACCCTACTC TGGCGGGACA CGGACACTAG TCGACGGCGA 
AGAGACAGCC TCACGTGGAA GGAGATCAAA GCAGATCGTG CGGCCATGCT ACGCGAAGGA 
GTTGATTCAA GCTCTCTAAA GACCCAGAAA CTTCGCACGC GGTTGGGCAA AAGTGACAGT 
TCGTCTTACA GTGAGGATAG CGATGGCGAT CAGGATGAGA AAGCAGACAA CGGTGGTGCT 
GGTGGTGGCT CTAGTACGGA CACCAGTCTC TGTGATGACG ATGATCCCAA GTCAAGCGAA 
AAAAGCCCCA AGCGTAAGGC GAAGGTGGCA CGTAAGCTCA AAGAGAAGAG TCAGCATTTG 
GGGTCTCGGG AGTCCAGCGC AGAGCGTCAA CGACCGAAAT CCTGGGCACC AGGCACGCAT 
GAGATACCCT TTATGTTGAT GGGCATGGAC AGCGGTGATG AAAAGGAAGC CAAAAAGGAG 
GAGCAAGACC TGGAGGAAGA CAGTGCCACT GAAAGTGGTC GTTTTAGCTA TCCTAAGGAA 
CTAGATTGGA CTCAGGCGGC AGATAAAGTA GCAAGTTGTG AACTAGAACC ATTGGATATT 
GACAAGATAC TGCCTAACAC TGCTATTTAT CTAAGCACAA TCAGTCTAAG TCCGCCCAAG 
ACGGAAGATG TGGGAGATCA GGATGTGATA GAGGAACAAC CCATTGTGAA GCAGTTTGAC 
GTTAGTGATT CTGGGGTAAC AAATCAATAT TTTGAGCGTG TCAACAGCGT TGAGCGTCCC 
AATAGGTTGG AGCTCTCATA CTCTCTAAAC GAGGAGGAGG ACTCTACAAT GTTAAAAGAC 
GATCGTCAAA GTTCGGATTT TGAATTGAAA ATTGAACCCG AACCTTCCAC TTTAAATAAG 
GTCCCACAAA TTCGGGATGA TAACATACCC GGTGAAAACA AAGATGACTA CAAGGAGCTT 
TTGAGCATGA CGATAGAAGA GCATAAGCCA CCAGCAGCTA CAATCACCAG CCAACTGAAT 
GCGAGTCGAA AGCGTCGGGA TCCCAGGCGT AAAACGCTAA CCAGATCCTC CACAATCGAG 
ATTGAGGAAC GTTTTCAGGC CCTCGAACGA ACACTGAGCC AAGATCAACC GATTCATAAG 
TACATACCGA GCACTGCAGC TCTCGAAGAA CGCTATAATA GTCTAGAAAA ACAGTTGAGT 
GCCGAGAAAC AGCGGAAGGA GGAGGATCTG GATCGTATAC CTTCTACAGC TGATCTAGAA 
TCACGATTTG ATGCCTTAAC GAAACAAATG AGTTCCAGCG AATCGAGCAA TTCCAAGGCC 
CCACTCGATC TTAATAAAGA TCAGCAGCAG CCTGGCGAGA AATCCAAAGA AACAGAAAGA 
AAATCAAACA AAAACCAAAG CAAAGAAGGG GAGAAACAAA CCCAATCGGA ACCTAAAGAA 
ACTGCATCGG AAAAGGAAAG TCCTGGCCAA AAAGAAGAAA CCAAAGAAAT TGAAGAAGAA 
CAACAGCCAC GTCTCAAGAA ACTGCCCTCT ACAGCCGAAC TTGAGGATCG ATTTAATGCT 
TTAGAACGAA AAATGAGCGT ACAAAAATCT AGTTCGGCAA AGACAAAGAA AGAGCCACCC 
GATGAAGAAA AACCTGCCCA AAAAACTGAA GCTCAAACAA AGGAAACTAA ATCTAAACAA 
GAATCAAGCC CACAAAAGAC TCCAGAAAAC ATAGAGACAA GTGAAAAGAG CACGAAAATC 
AGTAAGGATT CAAATGAGGT TAAGGATAAA GCCGATGAGG TAAAACGAAA AGTTCCACCT 
TCAACCGAGG AGTTGGAAAA GCGTTTCAAT GCTTTGGAGA AACAACTTAG TACAACTAAT 
TTGGAAGCTG AGCAACCTAC TACTTCTAAG GCAAAGGAAA TCAAATCAGA ATTACCCAAG 
CAAACTGAAA ATGTAAAAGC GAACAAGCAA GAAATTCAAT CACAAAAATC CATCAAGTCT 
TTCGATGAAA AAGTTAAAGT TATTAATACA AAATTAACAG CCGATGAGCA GAAAATCCAA 
GACCACGACA AATTGGCCGA AGAAAAACGT AAAAGTCTTG ATGAGAAAAG TGAAAGAGCC 
AGTGGTCAGT TAAAAGAAAT GTTGCCTGAG AATGAAACCA TTAAATCAAA GACATCTCAA 
CCTGAAGTCG TCCGGGCTGA TGTTCAACCC AAAGAACTGG GTAAGCGAAG AGCTTCCGAA 
CCACCATCAA CAGAAGATTT GGAGAAACGC TATGAGACAC TTAAGCGTCG CATGAGTAGT 
AAAAATCATT TTGGTAATCT AACAGAATCA GCAGATGAGT CCATGTCAAG GCGAGATCAA 
GAAGTAATAT CCGAGACTTT AGAGACTGGA GAGCCGGAGA GAAAACAATC ACCGCCGACA 
ACAGAATATC TGGAAAGTAG GTATGAGAAG CTACATGGGG AAAACAAGAA AACAACGCCC 
AAGAAAGTGG ATGTGGCGAT TGAGGCGCAT ATATCACCAC CACCAGCTCC GCCACCACCT 
CCAAAGCCGA ATATCCTAGC TGATCCGGCT ATGCATAAGC AACGAGCTCT CATTGAAGAG 
CTACAGGCGA AAATTCAAGA GCAAGGACAG TCCACGCCTG GTGAAGAGAA TCTCAAGCCC 
AGCGAGATAA ATCCACAGCG CAAACAGAGG CAAGAGCAAC AACGTCTGCT ACAACGGCCC 
ACGACAATGG GAGATGAGAC CTCGGAAGCA CCTGCAAAGA CCGCTTACTA CAGATCGGGA 
AATTACGAAC CCTGGCAGCA GCAGCACCAG CAGAAGCAGC GAATGGTTCG TCGTTTCTCT 
GATTTGCCAT CAAGAGCCGA TCTAGAAAAT CGCTTACAAT TCCTGGAGAA GCAATTGTAC 
AAGAAATTCT ACAAGCAGCG CTGTGCAAGT GATTCCGAAG TTGCATCGCG GACGATCGAT 
GACGAAAAGC CCAGCACATC GGCAGCCCGT GTGGCGGAAG TTCAGCTGGA GCAACGTGTC 
CTGGCCCTAG AGAAGCAATT AAGTGAGAAT AGTCTAAAGC TGCTAGAGGC CATGCGTGTC 
AACGTGCCCA ACGTCGATGG CGGCTCTCCG CGAAGACTAA GCACGGATAC GATAGATGCC 
ACTGGCAAGG AACTGGTGCG GTATACACAG AACTTTGGTG AGCTGGACGA GTTGGGTGGA 
GATGGCTCAA ATAAGCCCAT TAACATTAGT ATCAATATCA AAATGATGCT CAACAAGGAA 
CAGCAACAAC AACGACAACA ACAACAGCAG CAGCAGCAGC AGGAGGAAAA GGCAGACACT 
AGGACCAGCA CAGAGGATTT AAGGATGCGC TTGGAGCAAT TGGAACAGCA ATTGATGGAA 
GAAAGAGCCA AGAACAATGG TTTTGTGGAT GTCGAAAGTG CTAATGATGG CGGAGCAGGA 
GAGGAGGCAA AGCAATTGGA TAAATCCGAA ACTTCAGTAC CGGAAGAACT AGAGGAAAAT 
AAAAAGCAAG AGAAGGACAG TCACAATCAA ACGGTAAAAA GTGATGAAGC TGAAATGACA 
GAAGATCCAA TTGTGGCCAC CACCGTGGAA ACCATTGAGC CGGAGACAGT GAAAGAAACA 
AAGACCTTAG AAAACGAGGA GATAGCTCAA GCAACCCAAG AGAAAGCTGC AAAAGCTGAG 
GAGATCGAAG AGACAACCAA AGATAAAATA AATGAATCAT CTGAAGTCAT TCCTAAAGTG 
GAGGAAACTA AACCGCCAAC AGAAACAGAA ACCCACTGCG AAGAGAAAAC TGTAGACTCT 
GACAAGATTA TTGTCAATGC CAGCTCTATT GAACCTCCGG TGGATCCCAA TAATAAGACC 
GTGGTCCTGC TTATGGATAA CGAACCTAGA GCTTCGAGAG TGCGCCGTTT GACCCGCGCC 
AATACCGAAG AACTGGAAGG TCTATTCCAG GCTTTAGAAA AACAACTGCA GGATCGCAAT 
CTAATCAAAT CTGAAGATGG GCGGTTAATA CGAGCAAGTA CGGAACAGAA ACAAACTGCA 
GAACAAGTAG AACAAGCTCA GGCTATAAGC GATCTAACTA AGGAAATCGA AGACTTTACC 
AGCGGTAAAC CAGACGCAAA AAGTGAAGGC GAAACAAAGG AAGATAAGCC AGCAGATGGC 
GGCGAAGAAC CCCCCGACGA TTATGATTGG GGTCCCAATC CGGTTAAGCA TCATTTGAAA 
CGCAAAACCG TATATTTGCC TTCAACCAAA GAGCTAGAGG CTCGTTTTCG GTCACTGGAA 
CGTCAAATTA AACTACTCGA AGATGTGGAG AAAATCGATG TGGAGCAGCG CTTAAATGAA 
ATTGAACGAA AGATTAAACT GCAGTATTCC TTGTCACATG AGAAAGATTT AAATAAATAT 
CTAGAGCTCT GCGAAGGCAA GGGCTTAGAT GAGGAAGAGG AGCCTATTGT AGATGCATCT 
CCTCCTTTAA CCAAGGATAC CCAGCGGTCC CGTAGTCCAG CACGTAAAGA AGCTTCAAAG 
TCGCCATATA CTTCACCATC GCGTAAGGCA GTTGCAAAAT CTCCTCATAC TTCTCCTGCA 
CGTAAGCCAG CTACAAAATC TCCTTATACC TCGCCTTCCC GTGCTAGTGA CAAAAAGCGG 
AGCCCTTATA CCTCGCCAGT ACGTCGACAG GCGCATCCCG GTGATTTACC CATCTCCGAT 
GACTTGGAGT ACAAGTATCG TGTGCTTGAT CTGGTACGTT CCAAATCCAA GGAGAACTTG 
TCGAAGCGTA AAACTGATCC CAATAAAAAG CCGCCCATTC ATCCTCTTGA AATGCTACTT 
GATCCGAGTC CCGATGACAG CGAGATACCC ACAACAGGGG AACTGGAGCA CCGCATACGT 
CTGCTGGATG AAAAGCTAAA GTCGCCCTGC CGCCATAAAT CTCGCTCACG TTCACCCACC 
ATCGATGACA TGAAACGAAA GAAGCTCTTG GAGGAAAAGC GACCCAAATC GCCGGTTCAT 
ACATTGGAAC GTTTGGTCAG TTCACCTAAC AGACCAGAGC CACCCACTGA GGAGGAACTA 
GAACAGCGAA TGCGTGCCTT GGAAGACGAA AAGCGTTTCG ACTTCAAGAC CCAAAAGGAT 
TACAAGGCAT TCAATCAAAA GCTTAAAGAT GTCATCTCAC CTTCCCTATC TTTCGAAGAG 
TTCAAGGCGG CCAAATCGCG TGAACAGAGT CCACGGCGTC AAGGAGCCAC AACACCCAAA 
TCAGCAATGC GACGCGACGA TACAGATGAT TACCGAGGAC GCGAGGAGAC ACCCCATTAT 
CGGCCCACTA GTCCTAAGGT GATACGCTTT CGAGATGAGG ATGAGGATTA TTACAAGGAG 
AGCCAGCGTC CGAAGTCTCG ACAGTCCAGC GAACGAATGA CTCTTGCAGA TCGCAGCTAC 
AGCAGCAGCA TTGCAGCAGC AACGGCCAGC AGCTCCGAGG GTCTCGATGC CCTGGGTAGT 
CGCCTAATGA GAGAAACCTC GCCAATTACC CGCACTGGGA CTCATACTGG TGTGCCGTTA 
CGGACTGGAG AGAATATCAA TGATCGTCTA AGTTCTATCA AGGATACCAT CAAATCCATT 
GACACTCTAT GCGAGGAGAA ACCGTATCAG AAGGAGAAAT GCCAACGTTA TATTGATTCC 
CTCTTCTCAG ATTCATTGCA TTTCGGTTGT AAGAAGAATT CCATGGAGGA GCTAACGCAC 
AGTCGCAGCG AGAGTCGAGG ACGTACAGCA CAACGATCGA GTGACTATAC GCCGGCCATT 
CGAGTCACCT CAGAGCATCG ATCTCTGGGC TCGGCCGACT CGAAGCGCAC AAGTCCATTG 
CGGGCATCCA GTCCGTTGCA TCATCATCGC TCGCATAGGG ATATCAGTAG GGAGTTATCG 
CCGCGCAGGC GTCGTGATGC CGAGGATATG GAGGAGCGCG AAAGTAGTAG GGTAAGACGT 
GATAATATGT TGCCAAATTA TTTTCTCGAT AATCGTAGCG ATTTAAGTAG CGGCAGTAGT 
TTAACTAAGT TTCATAAAGT AGATAGACAA CTAGAAGAAA CGTGCGCCAA GTACGAAGCT 
GACGATCGAC GCTCGGCCAG CAGAACACCA TTGAGTTCGC CATATGAGTC CAGAACAACA 
GCCACACGCT ACAACAACAA CAACAACACA GGCACAGGGA CAACAACAAC AACAACCGCC 
GATACATTTC AAAGGCCCGT ATCGCCCTAT CGCCAGCCCT ATGACCCATA TAGACGTAAT 
GTGAATGCCC CATCCCCGGT TCCGGTTTAT CAGCCAGCAA AGCTGGAAAT TCGTCATACG 
ACAGTCACAT CAACCTTCTA TGATCGTTTT CTCACCGAAA AACAAATTGA GAAGCAAACA 
CTCTCACGTC CACCCAGTCG TTCGCCTGTA GTCTCACCAT CGGCCAGCAT AGCCAAAAGC 
GATACTGATC TGCCGGGCAC AGTGAGCAGT GCCTCTGCTT CCGCTTCCGC CTCACAATTC 
GCTTCCTCCT ACTCCTCCGC CGCCTACAAG CCCACAGTAA CCACCGCAAT CATGTCCAGC 
AGTTATGCTG GTACTGCCAC CTCACATTTT ACTTCTTCGT TGCCCACAAG CAATTATTCA 
TATCTCATGA GCACTTCCAC CAACGCCACC ACCACCACCA CCTCTTCCTC CTCCACTACT 
GGTGGTTCGT CTGGGGGATA TGTGCCATAT AATTTCAGCA GCTCGTTTAG CAATCGTCAT 
AGTGATCCAA TTGCCAATTC TTCTGGCATC TCGAGCGGTA GCACTGGTGT CTATAATCCC 
ATGATGTCGT TCACTTTGCG TGAACCAACA AGTGCCTCGG GCAGTCTCTT GGGTCAATAT 
GCCTACAAAA GTAGTTTCGT CTCCAACTAC AGTTCGTCAT CCAATCAAAT GCCCAAGTCA 
CCATCCACCA GTGATCAGGA CAAGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_023179838.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023035606.1 Drosophila willistoni titin (LOC6649543), transcript variant X1, mRNA.

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CloneID	ODf27213
Clone ID Related Accession (Same CDS sequence)	XM_023179838.1
Accession Version	XM_023179838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	uncharacterized protein LOC6649543 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032872.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTT ACTTGTTGGA ACGTGTTTTG ATAGTGCGCG ATCGTTTGCA ACGGCATCAG 
GATGTGCTCT GTGAATTTGT CAATGCTGTT CTTATAGTGG AAAGTAACGA TGCACAGCCT 
AAAATGCGTT TTAGCCTTTC ACCACCGCCG CAGAAAGCTA TTACCGCCAG CGTAACCTCA 
ACCAAGAGTA GCACAAACAT TGACATTAGC TCCAGCTCCA GCACCATCAC CACCGCGGCT 
ACAACCAGCA CCACCAAAAC AACAACAACC ACTTTCTCCA CCACCACCAC CAACAACAAA 
AATAATATGC TGGGTCATGA GGGGAACAGC TCAAATTCAG CTGCCTCTAT TTCTGCATCA 
GCTGACGAAG ATGCCGCCTC GGATTATAAT CAATGGCTGC ATGCCATGAA ACTGGTGGCT 
CGTCTACCAG GAGGCACACC ACCCGAGTTT CGTCGCAAGC TCTGGCTCTC GTTGGCTGAC 
AAATATCTCA AGTCCAAGAA TGTTGATTGG ACGCAGCAGC GTGAGAAATG CTTTTGCGAG 
GAATGGCGTG AAGATGACGA AGAGCTGGGC ATACAAATTG TCAAGGATTT GCATCGCACT 
GGTTCTAATT TGTGCACTGG TCCCGCGGGC TCCATTAACC AGGCCAAGCT GAAGCGCATT 
CTACTCGGCT ATGCCCGCTA TAATCCCGAG GTGGGCTATT GTCAGGGCTT CAATATGCTG 
GGTGCTTTGA TTCTACAAGT CATGGACAAG GAAGAGGAGG AGTCCATGAA GGTCATGATC 
TATCTGGTGG AGGGTGTACT GCCAACGGGT TATTTCTATG GTTCAATGGG CGGTCTTCAG 
GCGGATATGG GAGTCTTTCG CGAGCTTATG CAAACGAAAC TGCCGCGTCT GGCCAAGCAT 
TTGCAGCGTT TGCAGGGAGG ACCCGTTGAG AATGCCTACG AACCGCCATT GACCAATGTC 
TTCACCATGC AATGGTTCCT CACCATGTTC TGCACCTGCC TACCCATGTC CTGTGTCCTG 
CGCGTCTGGG ATCTCGTTCT CATCGAAGGC AGTGATGTTC TATTGCGCAC TGCCCTCGTC 
CTGTGGAGTC TATTGGAAGA ACGTGTCCTT AATGCTCGCA CAGCTGATGA GTTCTATGGC 
AAAATGGGAT CTTTCTCGAG TGAATTGCTC AATGGTCATC TTATCGATTC AAATGGTCTA 
ATCGAAAAGG TTGTTAAACT CGGACCCATT GCCGATTTGC GTCATTTGAG GGATAAACAT 
CTATATAACA TAGCCCCATT GAGGCATAAA CAAGGCATAC AACTCTATTA CGATGACGAT 
GATACACATT CGGATGAGGA GCGCATGGCA GTGGCTACCG TGTGGGGTTT AAATTGGGGT 
CGCCGCGGTT CGGTGGGACC CGGAGCCCCA GCGGGTAGGC AGCCATCTGA ACAAAAGGAT 
CGTCTGGCCT TGGATATATC GTTGCTGAAG AAGCAATACG ATCGTCTTCG AGAACGTCAG 
AAGCAGGCCC ATGTCATCCT AACTACTGCA TGCTCAACAG CAACTCGTCA AACGCCTTCA 
GCGCAAAGCT CGGTGCCGGT TAATCAGTTA CTACAGGGTC GACACGCCAT TGTGACCAAC 
AGGGGCAAAC GGGTGAGTGC TCCCTTGGGA GCCATACCTC CAGCCCGAAA GCCTTCACTG 
CCAGCTGTAT TGCACGGAAA CGGAAAGCCT ATGATGGGGG AGAAGCAGTT GCGACGAGGA 
GAAACCCTAC TCTGGCGGGA CACGGACACT AGTCGACGGC GAAGAGACAG CCTCACGTGG 
AAGGAGATCA AAGCAGATCG TGCGGCCATG CTACGCGAAG GAGTTGATTC AAGCTCTCTA 
AAGACCCAGA AACTTCGCAC GCGGTTGGGC AAAAGTGACA GTTCGTCTTA CAGTGAGGAT 
AGCGATGGCG ATCAGGATGA GAAAGCAGAC AACGGTGGTG CTGGTGGTGG CTCTAGTACG 
GACACCAGTC TCTGTGATGA CGATGATCCC AAGTCAAGCG AAAAAAGCCC CAAGCGTAAG 
GCGAAGGTGG CACGTAAGCT CAAAGAGAAG AGTCAGCATT TGGGGTCTCG GGAGTCCAGC 
GCAGAGCGTC AACGACCGAA ATCCTGGGCA CCAGGCACGC ATGAGATACC CTTTATGTTG 
ATGGGCATGG ACAGCGGTGA TGAAAAGGAA GCCAAAAAGG AGGAGCAAGA CCTGGAGGAA 
GACAGTGCCA CTGAAAGTGG TCGTTTTAGC TATCCTAAGG AACTAGATTG GACTCAGGCG 
GCAGATAAAG TAGCAAGTTG TGAACTAGAA CCATTGGATA TTGACAAGAT ACTGCCTAAC 
ACTGCTATTT ATCTAAGCAC AATCAGTCTA AGTCCGCCCA AGACGGAAGA TGTGGGAGAT 
CAGGATGTGA TAGAGGAACA ACCCATTGTG AAGCAGTTTG ACGTTAGTGA TTCTGGGGTA 
ACAAATCAAT ATTTTGAGCG TGTCAACAGC GTTGAGCGTC CCAATAGGTT GGAGCTCTCA 
TACTCTCTAA ACGAGGAGGA GGACTCTACA ATGTTAAAAG ACGATCGTCA AAGTTCGGAT 
TTTGAATTGA AAATTGAACC CGAACCTTCC ACTTTAAATA AGGTCCCACA AATTCGGGAT 
GATAACATAC CCGGTGAAAA CAAAGATGAC TACAAGGAGC TTTTGAGCAT GACGATAGAA 
GAGCATAAGC CACCAGCAGC TACAATCACC AGCCAACTGA ATGCGAGTCG AAAGCGTCGG 
GATCCCAGGC GTAAAACGCT AACCAGATCC TCCACAATCG AGATTGAGGA ACGTTTTCAG 
GCCCTCGAAC GAACACTGAG CCAAGATCAA CCGATTCATA AGTACATACC GAGCACTGCA 
GCTCTCGAAG AACGCTATAA TAGTCTAGAA AAACAGTTGA GTGCCGAGAA ACAGCGGAAG 
GAGGAGGATC TGGATCGTAT ACCTTCTACA GCTGATCTAG AATCACGATT TGATGCCTTA 
ACGAAACAAA TGAGTTCCAG CGAATCGAGC AATTCCAAGG CCCCACTCGA TCTTAATAAA 
GATCAGCAGC AGCCTGGCGA GAAATCCAAA GAAACAGAAA GAAAATCAAA CAAAAACCAA 
AGCAAAGAAG GGGAGAAACA AACCCAATCG GAACCTAAAG AAACTGCATC GGAAAAGGAA 
AGTCCTGGCC AAAAAGAAGA AACCAAAGAA ATTGAAGAAG AACAACAGCC ACGTCTCAAG 
AAACTGCCCT CTACAGCCGA ACTTGAGGAT CGATTTAATG CTTTAGAACG AAAAATGAGC 
GTACAAAAAT CTAGTTCGGC AAAGACAAAG AAAGAGCCAC CCGATGAAGA AAAACCTGCC 
CAAAAAACTG AAGCTCAAAC AAAGGAAACT AAATCTAAAC AAGAATCAAG CCCACAAAAG 
ACTCCAGAAA ACATAGAGAC AAGTGAAAAG AGCACGAAAA TCAGTAAGGA TTCAAATGAG 
GTTAAGGATA AAGCCGATGA GGTAAAACGA AAAGTTCCAC CTTCAACCGA GGAGTTGGAA 
AAGCGTTTCA ATGCTTTGGA GAAACAACTT AGTACAACTA ATTTGGAAGC TGAGCAACCT 
ACTACTTCTA AGGCAAAGGA AATCAAATCA GAATTACCCA AGCAAACTGA AAATGTAAAA 
GCGAACAAGC AAGAAATTCA ATCACAAAAA TCCATCAAGT CTTTCGATGA AAAAGTTAAA 
GTTATTAATA CAAAATTAAC AGCCGATGAG CAGAAAATCC AAGACCACGA CAAATTGGCC 
GAAGAAAAAC GTAAAAGTCT TGATGAGAAA AGTGAAAGAG CCAGTGGTCA GTTAAAAGAA 
ATGTTGCCTG AGAATGAAAC CATTAAATCA AAGACATCTC AACCTGAAGT CGTCCGGGCT 
GATGTTCAAC CCAAAGAACT GGGTAAGCGA AGAGCTTCCG AACCACCATC AACAGAAGAT 
TTGGAGAAAC GCTATGAGAC ACTTAAGCGT CGCATGAGTA GTAAAAATCA TTTTGGTAAT 
CTAACAGAAT CAGCAGATGA GTCCATGTCA AGGCGAGATC AAGAAGTAAT ATCCGAGACT 
TTAGAGACTG GAGAGCCGGA GAGAAAACAA TCACCGCCGA CAACAGAATA TCTGGAAAGT 
AGGTATGAGA AGCTACATGG GGAAAACAAG AAAACAACGC CCAAGAAAGT GGATGTGGCG 
ATTGAGGCGC ATATATCACC ACCACCAGCT CCGCCACCAC CTCCAAAGCC GAATATCCTA 
GCTGATCCGG CTATGCATAA GCAACGAGCT CTCATTGAAG AGCTACAGGC GAAAATTCAA 
GAGCAAGGAC AGTCCACGCC TGGTGAAGAG AATCTCAAGC CCAGCGAGAT AAATCCACAG 
CGCAAACAGA GGCAAGAGCA ACAACGTCTG CTACAACGGC CCACGACAAT GGGAGATGAG 
ACCTCGGAAG CACCTGCAAA GACCGCTTAC TACAGATCGG GAAATTACGA ACCCTGGCAG 
CAGCAGCACC AGCAGAAGCA GCGAATGGTT CGTCGTTTCT CTGATTTGCC ATCAAGAGCC 
GATCTAGAAA ATCGCTTACA ATTCCTGGAG AAGCAATTGT ACAAGAAATT CTACAAGCAG 
CGCTGTGCAA GTGATTCCGA AGTTGCATCG CGGACGATCG ATGACGAAAA GCCCAGCACA 
TCGGCAGCCC GTGTGGCGGA AGTTCAGCTG GAGCAACGTG TCCTGGCCCT AGAGAAGCAA 
TTAAGTGAGA ATAGTCTAAA GCTGCTAGAG GCCATGCGTG TCAACGTGCC CAACGTCGAT 
GGCGGCTCTC CGCGAAGACT AAGCACGGAT ACGATAGATG CCACTGGCAA GGAACTGGTG 
CGGTATACAC AGAACTTTGG TGAGCTGGAC GAGTTGGGTG GAGATGGCTC AAATAAGCCC 
ATTAACATTA GTATCAATAT CAAAATGATG CTCAACAAGG AACAGCAACA ACAACGACAA 
CAACAACAGC AGCAGCAGCA GCAGGAGGAA AAGGCAGACA CTAGGACCAG CACAGAGGAT 
TTAAGGATGC GCTTGGAGCA ATTGGAACAG CAATTGATGG AAGAAAGAGC CAAGAACAAT 
GGTTTTGTGG ATGTCGAAAG TGCTAATGAT GGCGGAGCAG GAGAGGAGGC AAAGCAATTG 
GATAAATCCG AAACTTCAGT ACCGGAAGAA CTAGAGGAAA ATAAAAAGCA AGAGAAGGAC 
AGTCACAATC AAACGGTAAA AAGTGATGAA GCTGAAATGA CAGAAGATCC AATTGTGGCC 
ACCACCGTGG AAACCATTGA GCCGGAGACA GTGAAAGAAA CAAAGACCTT AGAAAACGAG 
GAGATAGCTC AAGCAACCCA AGAGAAAGCT GCAAAAGCTG AGGAGATCGA AGAGACAACC 
AAAGATAAAA TAAATGAATC ATCTGAAGTC ATTCCTAAAG TGGAGGAAAC TAAACCGCCA 
ACAGAAACAG AAACCCACTG CGAAGAGAAA ACTGTAGACT CTGACAAGAT TATTGTCAAT 
GCCAGCTCTA TTGAACCTCC GGTGGATCCC AATAATAAGA CCGTGGTCCT GCTTATGGAT 
AACGAACCTA GAGCTTCGAG AGTGCGCCGT TTGACCCGCG CCAATACCGA AGAACTGGAA 
GGTCTATTCC AGGCTTTAGA AAAACAACTG CAGGATCGCA ATCTAATCAA ATCTGAAGAT 
GGGCGGTTAA TACGAGCAAG TACGGAACAG AAACAAACTG CAGAACAAGT AGAACAAGCT 
CAGGCTATAA GCGATCTAAC TAAGGAAATC GAAGACTTTA CCAGCGGTAA ACCAGACGCA 
AAAAGTGAAG GCGAAACAAA GGAAGATAAG CCAGCAGATG GCGGCGAAGA ACCCCCCGAC 
GATTATGATT GGGGTCCCAA TCCGGTTAAG CATCATTTGA AACGCAAAAC CGTATATTTG 
CCTTCAACCA AAGAGCTAGA GGCTCGTTTT CGGTCACTGG AACGTCAAAT TAAACTACTC 
GAAGATGTGG AGAAAATCGA TGTGGAGCAG CGCTTAAATG AAATTGAACG AAAGATTAAA 
CTGCAGTATT CCTTGTCACA TGAGAAAGAT TTAAATAAAT ATCTAGAGCT CTGCGAAGGC 
AAGGGCTTAG ATGAGGAAGA GGAGCCTATT GTAGATGCAT CTCCTCCTTT AACCAAGGAT 
ACCCAGCGGT CCCGTAGTCC AGCACGTAAA GAAGCTTCAA AGTCGCCATA TACTTCACCA 
TCGCGTAAGG CAGTTGCAAA ATCTCCTCAT ACTTCTCCTG CACGTAAGCC AGCTACAAAA 
TCTCCTTATA CCTCGCCTTC CCGTGCTAGT GACAAAAAGC GGAGCCCTTA TACCTCGCCA 
GTACGTCGAC AGGCGCATCC CGGTGATTTA CCCATCTCCG ATGACTTGGA GTACAAGTAT 
CGTGTGCTTG ATCTGGTACG TTCCAAATCC AAGGAGAACT TGTCGAAGCG TAAAACTGAT 
CCCAATAAAA AGCCGCCCAT TCATCCTCTT GAAATGCTAC TTGATCCGAG TCCCGATGAC 
AGCGAGATAC CCACAACAGG GGAACTGGAG CACCGCATAC GTCTGCTGGA TGAAAAGCTA 
AAGTCGCCCT GCCGCCATAA ATCTCGCTCA CGTTCACCCA CCATCGATGA CATGAAACGA 
AAGAAGCTCT TGGAGGAAAA GCGACCCAAA TCGCCGGTTC ATACATTGGA ACGTTTGGTC 
AGTTCACCTA ACAGACCAGA GCCACCCACT GAGGAGGAAC TAGAACAGCG AATGCGTGCC 
TTGGAAGACG AAAAGCGTTT CGACTTCAAG ACCCAAAAGG ATTACAAGGC ATTCAATCAA 
AAGCTTAAAG ATGTCATCTC ACCTTCCCTA TCTTTCGAAG AGTTCAAGGC GGCCAAATCG 
CGTGAACAGA GTCCACGGCG TCAAGGAGCC ACAACACCCA AATCAGCAAT GCGACGCGAC 
GATACAGATG ATTACCGAGG ACGCGAGGAG ACACCCCATT ATCGGCCCAC TAGTCCTAAG 
GTGATACGCT TTCGAGATGA GGATGAGGAT TATTACAAGG AGAGCCAGCG TCCGAAGTCT 
CGACAGTCCA GCGAACGAAT GAAGACTCTT GCAGATCGCA GCTACAGCAG CAGCATTGCA 
GCAGCAACGG CCAGCAGCTC CGAGGGTCTC GATGCCCTGG GTAGTCGCCT AATGAGAGAA 
ACCTCGCCAA TTACCCGCAC TGGGACTCAT ACTGGTGTGC CGTTACGGAC TGGAGAGAAT 
ATCAATGATC GTCTAAGTTC TATCAAGGAT ACCATCAAAT CCATTGACAC TCTATGCGAG 
GAGAAACCGT ATCAGAAGGA GAAATGCCAA CGTTATATTG ATTCCCTCTT CTCAGATTCA 
TTGCATTTCG GTTGTAAGAA GAATTCCATG GAGGAGCTAA CGCACAGTCG CAGCGAGAGT 
CGAGGACGTA CAGCACAACG ATCGAGTGAC TATACGCCGG CCATTCGAGT CACCTCAGAG 
CATCGATCTC TGGGCTCGGC CGACTCGAAG CGCACAAGTC CATTGCGGGC ATCCAGTCCG 
TTGCATCATC ATCGCTCGCA TAGGGATATC AGTAGGGAGT TATCGCCGCG CAGGCGTCGT 
GATGCCGAGG ATATGGAGGA GCGCGAAAGT AGTAGGGTAA GACGTGATAA TATGTTGCCA 
AATTATTTTC TCGATAATCG TAGCGATTTA AGTAGCGGCA GTAGTTTAAC TAAGTTTCAT 
AAAGTAGATA GACAACTAGA AGAAACGTGC GCCAAGTACG AAGCTGACGA TCGACGCTCG 
GCCAGCAGAA CACCATTGAG TTCGCCATAT GAGTCCAGAA CAACAGCCAC ACGCTACAAC 
AACAACAACA ACACAGGCAC AGGGACAACA ACAACAACAA CCGCCGATAC ATTTCAAAGG 
CCCGTATCGC CCTATCGCCA GCCCTATGAC CCATATAGAC GTAATGTGAA TGCCCCATCC 
CCGGTTCCGG TTTATCAGCC AGCAAAGCTG GAAATTCGTC ATACGACAGT CACATCAACC 
TTCTATGATC GTTTTCTCAC CGAAAAACAA ATTGAGAAGC AAACACTCTC ACGTCCACCC 
AGTCGTTCGC CTGTAGTCTC ACCATCGGCC AGCATAGCCA AAAGCGATAC TGATCTGCCG 
GGCACAGTGA GCAGTGCCTC TGCTTCCGCT TCCGCCTCAC AATTCGCTTC CTCCTACTCC 
TCCGCCGCCT ACAAGCCCAC AGTAACCACC GCAATCATGT CCAGCAGTTA TGCTGGTACT 
GCCACCTCAC ATTTTACTTC TTCGTTGCCC ACAAGCAATT ATTCATATCT CATGAGCACT 
TCCACCAACG CCACCACCAC CACCACCTCT TCCTCCTCCA CTACTGGTGG TTCGTCTGGG 
GGATATGTGC CATATAATTT CAGCAGCTCG TTTAGCAATC GTCATAGTGA TCCAATTGCC 
AATTCTTCTG GCATCTCGAG CGGTAGCACT GGTGTCTATA ATCCCATGAT GTCGTTCACT 
TTGCGTGAAC CAACAAGTGC CTCGGGCAGT CTCTTGGGTC AATATGCCTA CAAAAGTAGT 
TTCGTCTCCA ACTACAGTTC GTCATCCAAT CAAATGCCCA AGTCACCATC CACCAGTGAT 
CAGGACAAGC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023035606.1
CDS	34..8688
Translation	MMGYLLERVLIVRDRLQRHQDVLCEFVNAVLIVESNDAQPKMRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMKTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_023179838.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni titin (LOC6649543), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023179838.1

ORF Insert Sequence:

ATGATGGGTT ACTTGTTGGA ACGTGTTTTG ATAGTGCGCG ATCGTTTGCA ACGGCATCAG 
GATGTGCTCT GTGAATTTGT CAATGCTGTT CTTATAGTGG AAAGTAACGA TGCACAGCCT 
AAAATGCGTT TTAGCCTTTC ACCACCGCCG CAGAAAGCTA TTACCGCCAG CGTAACCTCA 
ACCAAGAGTA GCACAAACAT TGACATTAGC TCCAGCTCCA GCACCATCAC CACCGCGGCT 
ACAACCAGCA CCACCAAAAC AACAACAACC ACTTTCTCCA CCACCACCAC CAACAACAAA 
AATAATATGC TGGGTCATGA GGGGAACAGC TCAAATTCAG CTGCCTCTAT TTCTGCATCA 
GCTGACGAAG ATGCCGCCTC GGATTATAAT CAATGGCTGC ATGCCATGAA ACTGGTGGCT 
CGTCTACCAG GAGGCACACC ACCCGAGTTT CGTCGCAAGC TCTGGCTCTC GTTGGCTGAC 
AAATATCTCA AGTCCAAGAA TGTTGATTGG ACGCAGCAGC GTGAGAAATG CTTTTGCGAG 
GAATGGCGTG AAGATGACGA AGAGCTGGGC ATACAAATTG TCAAGGATTT GCATCGCACT 
GGTTCTAATT TGTGCACTGG TCCCGCGGGC TCCATTAACC AGGCCAAGCT GAAGCGCATT 
CTACTCGGCT ATGCCCGCTA TAATCCCGAG GTGGGCTATT GTCAGGGCTT CAATATGCTG 
GGTGCTTTGA TTCTACAAGT CATGGACAAG GAAGAGGAGG AGTCCATGAA GGTCATGATC 
TATCTGGTGG AGGGTGTACT GCCAACGGGT TATTTCTATG GTTCAATGGG CGGTCTTCAG 
GCGGATATGG GAGTCTTTCG CGAGCTTATG CAAACGAAAC TGCCGCGTCT GGCCAAGCAT 
TTGCAGCGTT TGCAGGGAGG ACCCGTTGAG AATGCCTACG AACCGCCATT GACCAATGTC 
TTCACCATGC AATGGTTCCT CACCATGTTC TGCACCTGCC TACCCATGTC CTGTGTCCTG 
CGCGTCTGGG ATCTCGTTCT CATCGAAGGC AGTGATGTTC TATTGCGCAC TGCCCTCGTC 
CTGTGGAGTC TATTGGAAGA ACGTGTCCTT AATGCTCGCA CAGCTGATGA GTTCTATGGC 
AAAATGGGAT CTTTCTCGAG TGAATTGCTC AATGGTCATC TTATCGATTC AAATGGTCTA 
ATCGAAAAGG TTGTTAAACT CGGACCCATT GCCGATTTGC GTCATTTGAG GGATAAACAT 
CTATATAACA TAGCCCCATT GAGGCATAAA CAAGGCATAC AACTCTATTA CGATGACGAT 
GATACACATT CGGATGAGGA GCGCATGGCA GTGGCTACCG TGTGGGGTTT AAATTGGGGT 
CGCCGCGGTT CGGTGGGACC CGGAGCCCCA GCGGGTAGGC AGCCATCTGA ACAAAAGGAT 
CGTCTGGCCT TGGATATATC GTTGCTGAAG AAGCAATACG ATCGTCTTCG AGAACGTCAG 
AAGCAGGCCC ATGTCATCCT AACTACTGCA TGCTCAACAG CAACTCGTCA AACGCCTTCA 
GCGCAAAGCT CGGTGCCGGT TAATCAGTTA CTACAGGGTC GACACGCCAT TGTGACCAAC 
AGGGGCAAAC GGGTGAGTGC TCCCTTGGGA GCCATACCTC CAGCCCGAAA GCCTTCACTG 
CCAGCTGTAT TGCACGGAAA CGGAAAGCCT ATGATGGGGG AGAAGCAGTT GCGACGAGGA 
GAAACCCTAC TCTGGCGGGA CACGGACACT AGTCGACGGC GAAGAGACAG CCTCACGTGG 
AAGGAGATCA AAGCAGATCG TGCGGCCATG CTACGCGAAG GAGTTGATTC AAGCTCTCTA 
AAGACCCAGA AACTTCGCAC GCGGTTGGGC AAAAGTGACA GTTCGTCTTA CAGTGAGGAT 
AGCGATGGCG ATCAGGATGA GAAAGCAGAC AACGGTGGTG CTGGTGGTGG CTCTAGTACG 
GACACCAGTC TCTGTGATGA CGATGATCCC AAGTCAAGCG AAAAAAGCCC CAAGCGTAAG 
GCGAAGGTGG CACGTAAGCT CAAAGAGAAG AGTCAGCATT TGGGGTCTCG GGAGTCCAGC 
GCAGAGCGTC AACGACCGAA ATCCTGGGCA CCAGGCACGC ATGAGATACC CTTTATGTTG 
ATGGGCATGG ACAGCGGTGA TGAAAAGGAA GCCAAAAAGG AGGAGCAAGA CCTGGAGGAA 
GACAGTGCCA CTGAAAGTGG TCGTTTTAGC TATCCTAAGG AACTAGATTG GACTCAGGCG 
GCAGATAAAG TAGCAAGTTG TGAACTAGAA CCATTGGATA TTGACAAGAT ACTGCCTAAC 
ACTGCTATTT ATCTAAGCAC AATCAGTCTA AGTCCGCCCA AGACGGAAGA TGTGGGAGAT 
CAGGATGTGA TAGAGGAACA ACCCATTGTG AAGCAGTTTG ACGTTAGTGA TTCTGGGGTA 
ACAAATCAAT ATTTTGAGCG TGTCAACAGC GTTGAGCGTC CCAATAGGTT GGAGCTCTCA 
TACTCTCTAA ACGAGGAGGA GGACTCTACA ATGTTAAAAG ACGATCGTCA AAGTTCGGAT 
TTTGAATTGA AAATTGAACC CGAACCTTCC ACTTTAAATA AGGTCCCACA AATTCGGGAT 
GATAACATAC CCGGTGAAAA CAAAGATGAC TACAAGGAGC TTTTGAGCAT GACGATAGAA 
GAGCATAAGC CACCAGCAGC TACAATCACC AGCCAACTGA ATGCGAGTCG AAAGCGTCGG 
GATCCCAGGC GTAAAACGCT AACCAGATCC TCCACAATCG AGATTGAGGA ACGTTTTCAG 
GCCCTCGAAC GAACACTGAG CCAAGATCAA CCGATTCATA AGTACATACC GAGCACTGCA 
GCTCTCGAAG AACGCTATAA TAGTCTAGAA AAACAGTTGA GTGCCGAGAA ACAGCGGAAG 
GAGGAGGATC TGGATCGTAT ACCTTCTACA GCTGATCTAG AATCACGATT TGATGCCTTA 
ACGAAACAAA TGAGTTCCAG CGAATCGAGC AATTCCAAGG CCCCACTCGA TCTTAATAAA 
GATCAGCAGC AGCCTGGCGA GAAATCCAAA GAAACAGAAA GAAAATCAAA CAAAAACCAA 
AGCAAAGAAG GGGAGAAACA AACCCAATCG GAACCTAAAG AAACTGCATC GGAAAAGGAA 
AGTCCTGGCC AAAAAGAAGA AACCAAAGAA ATTGAAGAAG AACAACAGCC ACGTCTCAAG 
AAACTGCCCT CTACAGCCGA ACTTGAGGAT CGATTTAATG CTTTAGAACG AAAAATGAGC 
GTACAAAAAT CTAGTTCGGC AAAGACAAAG AAAGAGCCAC CCGATGAAGA AAAACCTGCC 
CAAAAAACTG AAGCTCAAAC AAAGGAAACT AAATCTAAAC AAGAATCAAG CCCACAAAAG 
ACTCCAGAAA ACATAGAGAC AAGTGAAAAG AGCACGAAAA TCAGTAAGGA TTCAAATGAG 
GTTAAGGATA AAGCCGATGA GGTAAAACGA AAAGTTCCAC CTTCAACCGA GGAGTTGGAA 
AAGCGTTTCA ATGCTTTGGA GAAACAACTT AGTACAACTA ATTTGGAAGC TGAGCAACCT 
ACTACTTCTA AGGCAAAGGA AATCAAATCA GAATTACCCA AGCAAACTGA AAATGTAAAA 
GCGAACAAGC AAGAAATTCA ATCACAAAAA TCCATCAAGT CTTTCGATGA AAAAGTTAAA 
GTTATTAATA CAAAATTAAC AGCCGATGAG CAGAAAATCC AAGACCACGA CAAATTGGCC 
GAAGAAAAAC GTAAAAGTCT TGATGAGAAA AGTGAAAGAG CCAGTGGTCA GTTAAAAGAA 
ATGTTGCCTG AGAATGAAAC CATTAAATCA AAGACATCTC AACCTGAAGT CGTCCGGGCT 
GATGTTCAAC CCAAAGAACT GGGTAAGCGA AGAGCTTCCG AACCACCATC AACAGAAGAT 
TTGGAGAAAC GCTATGAGAC ACTTAAGCGT CGCATGAGTA GTAAAAATCA TTTTGGTAAT 
CTAACAGAAT CAGCAGATGA GTCCATGTCA AGGCGAGATC AAGAAGTAAT ATCCGAGACT 
TTAGAGACTG GAGAGCCGGA GAGAAAACAA TCACCGCCGA CAACAGAATA TCTGGAAAGT 
AGGTATGAGA AGCTACATGG GGAAAACAAG AAAACAACGC CCAAGAAAGT GGATGTGGCG 
ATTGAGGCGC ATATATCACC ACCACCAGCT CCGCCACCAC CTCCAAAGCC GAATATCCTA 
GCTGATCCGG CTATGCATAA GCAACGAGCT CTCATTGAAG AGCTACAGGC GAAAATTCAA 
GAGCAAGGAC AGTCCACGCC TGGTGAAGAG AATCTCAAGC CCAGCGAGAT AAATCCACAG 
CGCAAACAGA GGCAAGAGCA ACAACGTCTG CTACAACGGC CCACGACAAT GGGAGATGAG 
ACCTCGGAAG CACCTGCAAA GACCGCTTAC TACAGATCGG GAAATTACGA ACCCTGGCAG 
CAGCAGCACC AGCAGAAGCA GCGAATGGTT CGTCGTTTCT CTGATTTGCC ATCAAGAGCC 
GATCTAGAAA ATCGCTTACA ATTCCTGGAG AAGCAATTGT ACAAGAAATT CTACAAGCAG 
CGCTGTGCAA GTGATTCCGA AGTTGCATCG CGGACGATCG ATGACGAAAA GCCCAGCACA 
TCGGCAGCCC GTGTGGCGGA AGTTCAGCTG GAGCAACGTG TCCTGGCCCT AGAGAAGCAA 
TTAAGTGAGA ATAGTCTAAA GCTGCTAGAG GCCATGCGTG TCAACGTGCC CAACGTCGAT 
GGCGGCTCTC CGCGAAGACT AAGCACGGAT ACGATAGATG CCACTGGCAA GGAACTGGTG 
CGGTATACAC AGAACTTTGG TGAGCTGGAC GAGTTGGGTG GAGATGGCTC AAATAAGCCC 
ATTAACATTA GTATCAATAT CAAAATGATG CTCAACAAGG AACAGCAACA ACAACGACAA 
CAACAACAGC AGCAGCAGCA GCAGGAGGAA AAGGCAGACA CTAGGACCAG CACAGAGGAT 
TTAAGGATGC GCTTGGAGCA ATTGGAACAG CAATTGATGG AAGAAAGAGC CAAGAACAAT 
GGTTTTGTGG ATGTCGAAAG TGCTAATGAT GGCGGAGCAG GAGAGGAGGC AAAGCAATTG 
GATAAATCCG AAACTTCAGT ACCGGAAGAA CTAGAGGAAA ATAAAAAGCA AGAGAAGGAC 
AGTCACAATC AAACGGTAAA AAGTGATGAA GCTGAAATGA CAGAAGATCC AATTGTGGCC 
ACCACCGTGG AAACCATTGA GCCGGAGACA GTGAAAGAAA CAAAGACCTT AGAAAACGAG 
GAGATAGCTC AAGCAACCCA AGAGAAAGCT GCAAAAGCTG AGGAGATCGA AGAGACAACC 
AAAGATAAAA TAAATGAATC ATCTGAAGTC ATTCCTAAAG TGGAGGAAAC TAAACCGCCA 
ACAGAAACAG AAACCCACTG CGAAGAGAAA ACTGTAGACT CTGACAAGAT TATTGTCAAT 
GCCAGCTCTA TTGAACCTCC GGTGGATCCC AATAATAAGA CCGTGGTCCT GCTTATGGAT 
AACGAACCTA GAGCTTCGAG AGTGCGCCGT TTGACCCGCG CCAATACCGA AGAACTGGAA 
GGTCTATTCC AGGCTTTAGA AAAACAACTG CAGGATCGCA ATCTAATCAA ATCTGAAGAT 
GGGCGGTTAA TACGAGCAAG TACGGAACAG AAACAAACTG CAGAACAAGT AGAACAAGCT 
CAGGCTATAA GCGATCTAAC TAAGGAAATC GAAGACTTTA CCAGCGGTAA ACCAGACGCA 
AAAAGTGAAG GCGAAACAAA GGAAGATAAG CCAGCAGATG GCGGCGAAGA ACCCCCCGAC 
GATTATGATT GGGGTCCCAA TCCGGTTAAG CATCATTTGA AACGCAAAAC CGTATATTTG 
CCTTCAACCA AAGAGCTAGA GGCTCGTTTT CGGTCACTGG AACGTCAAAT TAAACTACTC 
GAAGATGTGG AGAAAATCGA TGTGGAGCAG CGCTTAAATG AAATTGAACG AAAGATTAAA 
CTGCAGTATT CCTTGTCACA TGAGAAAGAT TTAAATAAAT ATCTAGAGCT CTGCGAAGGC 
AAGGGCTTAG ATGAGGAAGA GGAGCCTATT GTAGATGCAT CTCCTCCTTT AACCAAGGAT 
ACCCAGCGGT CCCGTAGTCC AGCACGTAAA GAAGCTTCAA AGTCGCCATA TACTTCACCA 
TCGCGTAAGG CAGTTGCAAA ATCTCCTCAT ACTTCTCCTG CACGTAAGCC AGCTACAAAA 
TCTCCTTATA CCTCGCCTTC CCGTGCTAGT GACAAAAAGC GGAGCCCTTA TACCTCGCCA 
GTACGTCGAC AGGCGCATCC CGGTGATTTA CCCATCTCCG ATGACTTGGA GTACAAGTAT 
CGTGTGCTTG ATCTGGTACG TTCCAAATCC AAGGAGAACT TGTCGAAGCG TAAAACTGAT 
CCCAATAAAA AGCCGCCCAT TCATCCTCTT GAAATGCTAC TTGATCCGAG TCCCGATGAC 
AGCGAGATAC CCACAACAGG GGAACTGGAG CACCGCATAC GTCTGCTGGA TGAAAAGCTA 
AAGTCGCCCT GCCGCCATAA ATCTCGCTCA CGTTCACCCA CCATCGATGA CATGAAACGA 
AAGAAGCTCT TGGAGGAAAA GCGACCCAAA TCGCCGGTTC ATACATTGGA ACGTTTGGTC 
AGTTCACCTA ACAGACCAGA GCCACCCACT GAGGAGGAAC TAGAACAGCG AATGCGTGCC 
TTGGAAGACG AAAAGCGTTT CGACTTCAAG ACCCAAAAGG ATTACAAGGC ATTCAATCAA 
AAGCTTAAAG ATGTCATCTC ACCTTCCCTA TCTTTCGAAG AGTTCAAGGC GGCCAAATCG 
CGTGAACAGA GTCCACGGCG TCAAGGAGCC ACAACACCCA AATCAGCAAT GCGACGCGAC 
GATACAGATG ATTACCGAGG ACGCGAGGAG ACACCCCATT ATCGGCCCAC TAGTCCTAAG 
GTGATACGCT TTCGAGATGA GGATGAGGAT TATTACAAGG AGAGCCAGCG TCCGAAGTCT 
CGACAGTCCA GCGAACGAAT GAAGACTCTT GCAGATCGCA GCTACAGCAG CAGCATTGCA 
GCAGCAACGG CCAGCAGCTC CGAGGGTCTC GATGCCCTGG GTAGTCGCCT AATGAGAGAA 
ACCTCGCCAA TTACCCGCAC TGGGACTCAT ACTGGTGTGC CGTTACGGAC TGGAGAGAAT 
ATCAATGATC GTCTAAGTTC TATCAAGGAT ACCATCAAAT CCATTGACAC TCTATGCGAG 
GAGAAACCGT ATCAGAAGGA GAAATGCCAA CGTTATATTG ATTCCCTCTT CTCAGATTCA 
TTGCATTTCG GTTGTAAGAA GAATTCCATG GAGGAGCTAA CGCACAGTCG CAGCGAGAGT 
CGAGGACGTA CAGCACAACG ATCGAGTGAC TATACGCCGG CCATTCGAGT CACCTCAGAG 
CATCGATCTC TGGGCTCGGC CGACTCGAAG CGCACAAGTC CATTGCGGGC ATCCAGTCCG 
TTGCATCATC ATCGCTCGCA TAGGGATATC AGTAGGGAGT TATCGCCGCG CAGGCGTCGT 
GATGCCGAGG ATATGGAGGA GCGCGAAAGT AGTAGGGTAA GACGTGATAA TATGTTGCCA 
AATTATTTTC TCGATAATCG TAGCGATTTA AGTAGCGGCA GTAGTTTAAC TAAGTTTCAT 
AAAGTAGATA GACAACTAGA AGAAACGTGC GCCAAGTACG AAGCTGACGA TCGACGCTCG 
GCCAGCAGAA CACCATTGAG TTCGCCATAT GAGTCCAGAA CAACAGCCAC ACGCTACAAC 
AACAACAACA ACACAGGCAC AGGGACAACA ACAACAACAA CCGCCGATAC ATTTCAAAGG 
CCCGTATCGC CCTATCGCCA GCCCTATGAC CCATATAGAC GTAATGTGAA TGCCCCATCC 
CCGGTTCCGG TTTATCAGCC AGCAAAGCTG GAAATTCGTC ATACGACAGT CACATCAACC 
TTCTATGATC GTTTTCTCAC CGAAAAACAA ATTGAGAAGC AAACACTCTC ACGTCCACCC 
AGTCGTTCGC CTGTAGTCTC ACCATCGGCC AGCATAGCCA AAAGCGATAC TGATCTGCCG 
GGCACAGTGA GCAGTGCCTC TGCTTCCGCT TCCGCCTCAC AATTCGCTTC CTCCTACTCC 
TCCGCCGCCT ACAAGCCCAC AGTAACCACC GCAATCATGT CCAGCAGTTA TGCTGGTACT 
GCCACCTCAC ATTTTACTTC TTCGTTGCCC ACAAGCAATT ATTCATATCT CATGAGCACT 
TCCACCAACG CCACCACCAC CACCACCTCT TCCTCCTCCA CTACTGGTGG TTCGTCTGGG 
GGATATGTGC CATATAATTT CAGCAGCTCG TTTAGCAATC GTCATAGTGA TCCAATTGCC 
AATTCTTCTG GCATCTCGAG CGGTAGCACT GGTGTCTATA ATCCCATGAT GTCGTTCACT 
TTGCGTGAAC CAACAAGTGC CTCGGGCAGT CTCTTGGGTC AATATGCCTA CAAAAGTAGT 
TTCGTCTCCA ACTACAGTTC GTCATCCAAT CAAATGCCCA AGTCACCATC CACCAGTGAT 
CAGGACAAGC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_023179839.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023035607.1 Drosophila willistoni titin (LOC6649543), transcript variant X2, mRNA.

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CloneID	ODf27214
Clone ID Related Accession (Same CDS sequence)	XM_023179839.1
Accession Version	XM_023179839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	uncharacterized protein LOC6649543 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032872.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGCA TACTGCACGG ATATTACGAT GCACAGCCTA AAATGCGTTT TAGCCTTTCA 
CCACCGCCGC AGAAAGCTAT TACCGCCAGC GTAACCTCAA CCAAGAGTAG CACAAACATT 
GACATTAGCT CCAGCTCCAG CACCATCACC ACCGCGGCTA CAACCAGCAC CACCAAAACA 
ACAACAACCA CTTTCTCCAC CACCACCACC AACAACAAAA ATAATATGCT GGGTCATGAG 
GGGAACAGCT CAAATTCAGC TGCCTCTATT TCTGCATCAG CTGACGAAGA TGCCGCCTCG 
GATTATAATC AATGGCTGCA TGCCATGAAA CTGGTGGCTC GTCTACCAGG AGGCACACCA 
CCCGAGTTTC GTCGCAAGCT CTGGCTCTCG TTGGCTGACA AATATCTCAA GTCCAAGAAT 
GTTGATTGGA CGCAGCAGCG TGAGAAATGC TTTTGCGAGG AATGGCGTGA AGATGACGAA 
GAGCTGGGCA TACAAATTGT CAAGGATTTG CATCGCACTG GTTCTAATTT GTGCACTGGT 
CCCGCGGGCT CCATTAACCA GGCCAAGCTG AAGCGCATTC TACTCGGCTA TGCCCGCTAT 
AATCCCGAGG TGGGCTATTG TCAGGGCTTC AATATGCTGG GTGCTTTGAT TCTACAAGTC 
ATGGACAAGG AAGAGGAGGA GTCCATGAAG GTCATGATCT ATCTGGTGGA GGGTGTACTG 
CCAACGGGTT ATTTCTATGG TTCAATGGGC GGTCTTCAGG CGGATATGGG AGTCTTTCGC 
GAGCTTATGC AAACGAAACT GCCGCGTCTG GCCAAGCATT TGCAGCGTTT GCAGGGAGGA 
CCCGTTGAGA ATGCCTACGA ACCGCCATTG ACCAATGTCT TCACCATGCA ATGGTTCCTC 
ACCATGTTCT GCACCTGCCT ACCCATGTCC TGTGTCCTGC GCGTCTGGGA TCTCGTTCTC 
ATCGAAGGCA GTGATGTTCT ATTGCGCACT GCCCTCGTCC TGTGGAGTCT ATTGGAAGAA 
CGTGTCCTTA ATGCTCGCAC AGCTGATGAG TTCTATGGCA AAATGGGATC TTTCTCGAGT 
GAATTGCTCA ATGGTCATCT TATCGATTCA AATGGTCTAA TCGAAAAGGT TGTTAAACTC 
GGACCCATTG CCGATTTGCG TCATTTGAGG GATAAACATC TATATAACAT AGCCCCATTG 
AGGCATAAAC AAGGCATACA ACTCTATTAC GATGACGATG ATACACATTC GGATGAGGAG 
CGCATGGCAG TGGCTACCGT GTGGGGTTTA AATTGGGGTC GCCGCGGTTC GGTGGGACCC 
GGAGCCCCAG CGGGTAGGCA GCCATCTGAA CAAAAGGATC GTCTGGCCTT GGATATATCG 
TTGCTGAAGA AGCAATACGA TCGTCTTCGA GAACGTCAGA AGCAGGCCCA TGTCATCCTA 
ACTACTGCAT GCTCAACAGC AACTCGTCAA ACGCCTTCAG CGCAAAGCTC GGTGCCGGTT 
AATCAGTTAC TACAGGGTCG ACACGCCATT GTGACCAACA GGGGCAAACG GGTGAGTGCT 
CCCTTGGGAG CCATACCTCC AGCCCGAAAG CCTTCACTGC CAGCTGTATT GCACGGAAAC 
GGAAAGCCTA TGATGGGGGA GAAGCAGTTG CGACGAGGAG AAACCCTACT CTGGCGGGAC 
ACGGACACTA GTCGACGGCG AAGAGACAGC CTCACGTGGA AGGAGATCAA AGCAGATCGT 
GCGGCCATGC TACGCGAAGG AGTTGATTCA AGCTCTCTAA AGACCCAGAA ACTTCGCACG 
CGGTTGGGCA AAAGTGACAG TTCGTCTTAC AGTGAGGATA GCGATGGCGA TCAGGATGAG 
AAAGCAGACA ACGGTGGTGC TGGTGGTGGC TCTAGTACGG ACACCAGTCT CTGTGATGAC 
GATGATCCCA AGTCAAGCGA AAAAAGCCCC AAGCGTAAGG CGAAGGTGGC ACGTAAGCTC 
AAAGAGAAGA GTCAGCATTT GGGGTCTCGG GAGTCCAGCG CAGAGCGTCA ACGACCGAAA 
TCCTGGGCAC CAGGCACGCA TGAGATACCC TTTATGTTGA TGGGCATGGA CAGCGGTGAT 
GAAAAGGAAG CCAAAAAGGA GGAGCAAGAC CTGGAGGAAG ACAGTGCCAC TGAAAGTGGT 
CGTTTTAGCT ATCCTAAGGA ACTAGATTGG ACTCAGGCGG CAGATAAAGT AGCAAGTTGT 
GAACTAGAAC CATTGGATAT TGACAAGATA CTGCCTAACA CTGCTATTTA TCTAAGCACA 
ATCAGTCTAA GTCCGCCCAA GACGGAAGAT GTGGGAGATC AGGATGTGAT AGAGGAACAA 
CCCATTGTGA AGCAGTTTGA CGTTAGTGAT TCTGGGGTAA CAAATCAATA TTTTGAGCGT 
GTCAACAGCG TTGAGCGTCC CAATAGGTTG GAGCTCTCAT ACTCTCTAAA CGAGGAGGAG 
GACTCTACAA TGTTAAAAGA CGATCGTCAA AGTTCGGATT TTGAATTGAA AATTGAACCC 
GAACCTTCCA CTTTAAATAA GGTCCCACAA ATTCGGGATG ATAACATACC CGGTGAAAAC 
AAAGATGACT ACAAGGAGCT TTTGAGCATG ACGATAGAAG AGCATAAGCC ACCAGCAGCT 
ACAATCACCA GCCAACTGAA TGCGAGTCGA AAGCGTCGGG ATCCCAGGCG TAAAACGCTA 
ACCAGATCCT CCACAATCGA GATTGAGGAA CGTTTTCAGG CCCTCGAACG AACACTGAGC 
CAAGATCAAC CGATTCATAA GTACATACCG AGCACTGCAG CTCTCGAAGA ACGCTATAAT 
AGTCTAGAAA AACAGTTGAG TGCCGAGAAA CAGCGGAAGG AGGAGGATCT GGATCGTATA 
CCTTCTACAG CTGATCTAGA ATCACGATTT GATGCCTTAA CGAAACAAAT GAGTTCCAGC 
GAATCGAGCA ATTCCAAGGC CCCACTCGAT CTTAATAAAG ATCAGCAGCA GCCTGGCGAG 
AAATCCAAAG AAACAGAAAG AAAATCAAAC AAAAACCAAA GCAAAGAAGG GGAGAAACAA 
ACCCAATCGG AACCTAAAGA AACTGCATCG GAAAAGGAAA GTCCTGGCCA AAAAGAAGAA 
ACCAAAGAAA TTGAAGAAGA ACAACAGCCA CGTCTCAAGA AACTGCCCTC TACAGCCGAA 
CTTGAGGATC GATTTAATGC TTTAGAACGA AAAATGAGCG TACAAAAATC TAGTTCGGCA 
AAGACAAAGA AAGAGCCACC CGATGAAGAA AAACCTGCCC AAAAAACTGA AGCTCAAACA 
AAGGAAACTA AATCTAAACA AGAATCAAGC CCACAAAAGA CTCCAGAAAA CATAGAGACA 
AGTGAAAAGA GCACGAAAAT CAGTAAGGAT TCAAATGAGG TTAAGGATAA AGCCGATGAG 
GTAAAACGAA AAGTTCCACC TTCAACCGAG GAGTTGGAAA AGCGTTTCAA TGCTTTGGAG 
AAACAACTTA GTACAACTAA TTTGGAAGCT GAGCAACCTA CTACTTCTAA GGCAAAGGAA 
ATCAAATCAG AATTACCCAA GCAAACTGAA AATGTAAAAG CGAACAAGCA AGAAATTCAA 
TCACAAAAAT CCATCAAGTC TTTCGATGAA AAAGTTAAAG TTATTAATAC AAAATTAACA 
GCCGATGAGC AGAAAATCCA AGACCACGAC AAATTGGCCG AAGAAAAACG TAAAAGTCTT 
GATGAGAAAA GTGAAAGAGC CAGTGGTCAG TTAAAAGAAA TGTTGCCTGA GAATGAAACC 
ATTAAATCAA AGACATCTCA ACCTGAAGTC GTCCGGGCTG ATGTTCAACC CAAAGAACTG 
GGTAAGCGAA GAGCTTCCGA ACCACCATCA ACAGAAGATT TGGAGAAACG CTATGAGACA 
CTTAAGCGTC GCATGAGTAG TAAAAATCAT TTTGGTAATC TAACAGAATC AGCAGATGAG 
TCCATGTCAA GGCGAGATCA AGAAGTAATA TCCGAGACTT TAGAGACTGG AGAGCCGGAG 
AGAAAACAAT CACCGCCGAC AACAGAATAT CTGGAAAGTA GGTATGAGAA GCTACATGGG 
GAAAACAAGA AAACAACGCC CAAGAAAGTG GATGTGGCGA TTGAGGCGCA TATATCACCA 
CCACCAGCTC CGCCACCACC TCCAAAGCCG AATATCCTAG CTGATCCGGC TATGCATAAG 
CAACGAGCTC TCATTGAAGA GCTACAGGCG AAAATTCAAG AGCAAGGACA GTCCACGCCT 
GGTGAAGAGA ATCTCAAGCC CAGCGAGATA AATCCACAGC GCAAACAGAG GCAAGAGCAA 
CAACGTCTGC TACAACGGCC CACGACAATG GGAGATGAGA CCTCGGAAGC ACCTGCAAAG 
ACCGCTTACT ACAGATCGGG AAATTACGAA CCCTGGCAGC AGCAGCACCA GCAGAAGCAG 
CGAATGGTTC GTCGTTTCTC TGATTTGCCA TCAAGAGCCG ATCTAGAAAA TCGCTTACAA 
TTCCTGGAGA AGCAATTGTA CAAGAAATTC TACAAGCAGC GCTGTGCAAG TGATTCCGAA 
GTTGCATCGC GGACGATCGA TGACGAAAAG CCCAGCACAT CGGCAGCCCG TGTGGCGGAA 
GTTCAGCTGG AGCAACGTGT CCTGGCCCTA GAGAAGCAAT TAAGTGAGAA TAGTCTAAAG 
CTGCTAGAGG CCATGCGTGT CAACGTGCCC AACGTCGATG GCGGCTCTCC GCGAAGACTA 
AGCACGGATA CGATAGATGC CACTGGCAAG GAACTGGTGC GGTATACACA GAACTTTGGT 
GAGCTGGACG AGTTGGGTGG AGATGGCTCA AATAAGCCCA TTAACATTAG TATCAATATC 
AAAATGATGC TCAACAAGGA ACAGCAACAA CAACGACAAC AACAACAGCA GCAGCAGCAG 
CAGGAGGAAA AGGCAGACAC TAGGACCAGC ACAGAGGATT TAAGGATGCG CTTGGAGCAA 
TTGGAACAGC AATTGATGGA AGAAAGAGCC AAGAACAATG GTTTTGTGGA TGTCGAAAGT 
GCTAATGATG GCGGAGCAGG AGAGGAGGCA AAGCAATTGG ATAAATCCGA AACTTCAGTA 
CCGGAAGAAC TAGAGGAAAA TAAAAAGCAA GAGAAGGACA GTCACAATCA AACGGTAAAA 
AGTGATGAAG CTGAAATGAC AGAAGATCCA ATTGTGGCCA CCACCGTGGA AACCATTGAG 
CCGGAGACAG TGAAAGAAAC AAAGACCTTA GAAAACGAGG AGATAGCTCA AGCAACCCAA 
GAGAAAGCTG CAAAAGCTGA GGAGATCGAA GAGACAACCA AAGATAAAAT AAATGAATCA 
TCTGAAGTCA TTCCTAAAGT GGAGGAAACT AAACCGCCAA CAGAAACAGA AACCCACTGC 
GAAGAGAAAA CTGTAGACTC TGACAAGATT ATTGTCAATG CCAGCTCTAT TGAACCTCCG 
GTGGATCCCA ATAATAAGAC CGTGGTCCTG CTTATGGATA ACGAACCTAG AGCTTCGAGA 
GTGCGCCGTT TGACCCGCGC CAATACCGAA GAACTGGAAG GTCTATTCCA GGCTTTAGAA 
AAACAACTGC AGGATCGCAA TCTAATCAAA TCTGAAGATG GGCGGTTAAT ACGAGCAAGT 
ACGGAACAGA AACAAACTGC AGAACAAGTA GAACAAGCTC AGGCTATAAG CGATCTAACT 
AAGGAAATCG AAGACTTTAC CAGCGGTAAA CCAGACGCAA AAAGTGAAGG CGAAACAAAG 
GAAGATAAGC CAGCAGATGG CGGCGAAGAA CCCCCCGACG ATTATGATTG GGGTCCCAAT 
CCGGTTAAGC ATCATTTGAA ACGCAAAACC GTATATTTGC CTTCAACCAA AGAGCTAGAG 
GCTCGTTTTC GGTCACTGGA ACGTCAAATT AAACTACTCG AAGATGTGGA GAAAATCGAT 
GTGGAGCAGC GCTTAAATGA AATTGAACGA AAGATTAAAC TGCAGTATTC CTTGTCACAT 
GAGAAAGATT TAAATAAATA TCTAGAGCTC TGCGAAGGCA AGGGCTTAGA TGAGGAAGAG 
GAGCCTATTG TAGATGCATC TCCTCCTTTA ACCAAGGATA CCCAGCGGTC CCGTAGTCCA 
GCACGTAAAG AAGCTTCAAA GTCGCCATAT ACTTCACCAT CGCGTAAGGC AGTTGCAAAA 
TCTCCTCATA CTTCTCCTGC ACGTAAGCCA GCTACAAAAT CTCCTTATAC CTCGCCTTCC 
CGTGCTAGTG ACAAAAAGCG GAGCCCTTAT ACCTCGCCAG TACGTCGACA GGCGCATCCC 
GGTGATTTAC CCATCTCCGA TGACTTGGAG TACAAGTATC GTGTGCTTGA TCTGGTACGT 
TCCAAATCCA AGGAGAACTT GTCGAAGCGT AAAACTGATC CCAATAAAAA GCCGCCCATT 
CATCCTCTTG AAATGCTACT TGATCCGAGT CCCGATGACA GCGAGATACC CACAACAGGG 
GAACTGGAGC ACCGCATACG TCTGCTGGAT GAAAAGCTAA AGTCGCCCTG CCGCCATAAA 
TCTCGCTCAC GTTCACCCAC CATCGATGAC ATGAAACGAA AGAAGCTCTT GGAGGAAAAG 
CGACCCAAAT CGCCGGTTCA TACATTGGAA CGTTTGGTCA GTTCACCTAA CAGACCAGAG 
CCACCCACTG AGGAGGAACT AGAACAGCGA ATGCGTGCCT TGGAAGACGA AAAGCGTTTC 
GACTTCAAGA CCCAAAAGGA TTACAAGGCA TTCAATCAAA AGCTTAAAGA TGTCATCTCA 
CCTTCCCTAT CTTTCGAAGA GTTCAAGGCG GCCAAATCGC GTGAACAGAG TCCACGGCGT 
CAAGGAGCCA CAACACCCAA ATCAGCAATG CGACGCGACG ATACAGATGA TTACCGAGGA 
CGCGAGGAGA CACCCCATTA TCGGCCCACT AGTCCTAAGG TGATACGCTT TCGAGATGAG 
GATGAGGATT ATTACAAGGA GAGCCAGCGT CCGAAGTCTC GACAGTCCAG CGAACGAATG 
AAGACTCTTG CAGATCGCAG CTACAGCAGC AGCATTGCAG CAGCAACGGC CAGCAGCTCC 
GAGGGTCTCG ATGCCCTGGG TAGTCGCCTA ATGAGAGAAA CCTCGCCAAT TACCCGCACT 
GGGACTCATA CTGGTGTGCC GTTACGGACT GGAGAGAATA TCAATGATCG TCTAAGTTCT 
ATCAAGGATA CCATCAAATC CATTGACACT CTATGCGAGG AGAAACCGTA TCAGAAGGAG 
AAATGCCAAC GTTATATTGA TTCCCTCTTC TCAGATTCAT TGCATTTCGG TTGTAAGAAG 
AATTCCATGG AGGAGCTAAC GCACAGTCGC AGCGAGAGTC GAGGACGTAC AGCACAACGA 
TCGAGTGACT ATACGCCGGC CATTCGAGTC ACCTCAGAGC ATCGATCTCT GGGCTCGGCC 
GACTCGAAGC GCACAAGTCC ATTGCGGGCA TCCAGTCCGT TGCATCATCA TCGCTCGCAT 
AGGGATATCA GTAGGGAGTT ATCGCCGCGC AGGCGTCGTG ATGCCGAGGA TATGGAGGAG 
CGCGAAAGTA GTAGGGTAAG ACGTGATAAT ATGTTGCCAA ATTATTTTCT CGATAATCGT 
AGCGATTTAA GTAGCGGCAG TAGTTTAACT AAGTTTCATA AAGTAGATAG ACAACTAGAA 
GAAACGTGCG CCAAGTACGA AGCTGACGAT CGACGCTCGG CCAGCAGAAC ACCATTGAGT 
TCGCCATATG AGTCCAGAAC AACAGCCACA CGCTACAACA ACAACAACAA CACAGGCACA 
GGGACAACAA CAACAACAAC CGCCGATACA TTTCAAAGGC CCGTATCGCC CTATCGCCAG 
CCCTATGACC CATATAGACG TAATGTGAAT GCCCCATCCC CGGTTCCGGT TTATCAGCCA 
GCAAAGCTGG AAATTCGTCA TACGACAGTC ACATCAACCT TCTATGATCG TTTTCTCACC 
GAAAAACAAA TTGAGAAGCA AACACTCTCA CGTCCACCCA GTCGTTCGCC TGTAGTCTCA 
CCATCGGCCA GCATAGCCAA AAGCGATACT GATCTGCCGG GCACAGTGAG CAGTGCCTCT 
GCTTCCGCTT CCGCCTCACA ATTCGCTTCC TCCTACTCCT CCGCCGCCTA CAAGCCCACA 
GTAACCACCG CAATCATGTC CAGCAGTTAT GCTGGTACTG CCACCTCACA TTTTACTTCT 
TCGTTGCCCA CAAGCAATTA TTCATATCTC ATGAGCACTT CCACCAACGC CACCACCACC 
ACCACCTCTT CCTCCTCCAC TACTGGTGGT TCGTCTGGGG GATATGTGCC ATATAATTTC 
AGCAGCTCGT TTAGCAATCG TCATAGTGAT CCAATTGCCA ATTCTTCTGG CATCTCGAGC 
GGTAGCACTG GTGTCTATAA TCCCATGATG TCGTTCACTT TGCGTGAACC AACAAGTGCC 
TCGGGCAGTC TCTTGGGTCA ATATGCCTAC AAAAGTAGTT TCGTCTCCAA CTACAGTTCG 
TCATCCAATC AAATGCCCAA GTCACCATCC ACCAGTGATC AGGACAAGCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023035607.1
CDS	77..8650
Translation	MVCILHGYYDAQPKMRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMKTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_023179839.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni titin (LOC6649543), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023179839.1

ORF Insert Sequence:

ATGGTTTGCA TACTGCACGG ATATTACGAT GCACAGCCTA AAATGCGTTT TAGCCTTTCA 
CCACCGCCGC AGAAAGCTAT TACCGCCAGC GTAACCTCAA CCAAGAGTAG CACAAACATT 
GACATTAGCT CCAGCTCCAG CACCATCACC ACCGCGGCTA CAACCAGCAC CACCAAAACA 
ACAACAACCA CTTTCTCCAC CACCACCACC AACAACAAAA ATAATATGCT GGGTCATGAG 
GGGAACAGCT CAAATTCAGC TGCCTCTATT TCTGCATCAG CTGACGAAGA TGCCGCCTCG 
GATTATAATC AATGGCTGCA TGCCATGAAA CTGGTGGCTC GTCTACCAGG AGGCACACCA 
CCCGAGTTTC GTCGCAAGCT CTGGCTCTCG TTGGCTGACA AATATCTCAA GTCCAAGAAT 
GTTGATTGGA CGCAGCAGCG TGAGAAATGC TTTTGCGAGG AATGGCGTGA AGATGACGAA 
GAGCTGGGCA TACAAATTGT CAAGGATTTG CATCGCACTG GTTCTAATTT GTGCACTGGT 
CCCGCGGGCT CCATTAACCA GGCCAAGCTG AAGCGCATTC TACTCGGCTA TGCCCGCTAT 
AATCCCGAGG TGGGCTATTG TCAGGGCTTC AATATGCTGG GTGCTTTGAT TCTACAAGTC 
ATGGACAAGG AAGAGGAGGA GTCCATGAAG GTCATGATCT ATCTGGTGGA GGGTGTACTG 
CCAACGGGTT ATTTCTATGG TTCAATGGGC GGTCTTCAGG CGGATATGGG AGTCTTTCGC 
GAGCTTATGC AAACGAAACT GCCGCGTCTG GCCAAGCATT TGCAGCGTTT GCAGGGAGGA 
CCCGTTGAGA ATGCCTACGA ACCGCCATTG ACCAATGTCT TCACCATGCA ATGGTTCCTC 
ACCATGTTCT GCACCTGCCT ACCCATGTCC TGTGTCCTGC GCGTCTGGGA TCTCGTTCTC 
ATCGAAGGCA GTGATGTTCT ATTGCGCACT GCCCTCGTCC TGTGGAGTCT ATTGGAAGAA 
CGTGTCCTTA ATGCTCGCAC AGCTGATGAG TTCTATGGCA AAATGGGATC TTTCTCGAGT 
GAATTGCTCA ATGGTCATCT TATCGATTCA AATGGTCTAA TCGAAAAGGT TGTTAAACTC 
GGACCCATTG CCGATTTGCG TCATTTGAGG GATAAACATC TATATAACAT AGCCCCATTG 
AGGCATAAAC AAGGCATACA ACTCTATTAC GATGACGATG ATACACATTC GGATGAGGAG 
CGCATGGCAG TGGCTACCGT GTGGGGTTTA AATTGGGGTC GCCGCGGTTC GGTGGGACCC 
GGAGCCCCAG CGGGTAGGCA GCCATCTGAA CAAAAGGATC GTCTGGCCTT GGATATATCG 
TTGCTGAAGA AGCAATACGA TCGTCTTCGA GAACGTCAGA AGCAGGCCCA TGTCATCCTA 
ACTACTGCAT GCTCAACAGC AACTCGTCAA ACGCCTTCAG CGCAAAGCTC GGTGCCGGTT 
AATCAGTTAC TACAGGGTCG ACACGCCATT GTGACCAACA GGGGCAAACG GGTGAGTGCT 
CCCTTGGGAG CCATACCTCC AGCCCGAAAG CCTTCACTGC CAGCTGTATT GCACGGAAAC 
GGAAAGCCTA TGATGGGGGA GAAGCAGTTG CGACGAGGAG AAACCCTACT CTGGCGGGAC 
ACGGACACTA GTCGACGGCG AAGAGACAGC CTCACGTGGA AGGAGATCAA AGCAGATCGT 
GCGGCCATGC TACGCGAAGG AGTTGATTCA AGCTCTCTAA AGACCCAGAA ACTTCGCACG 
CGGTTGGGCA AAAGTGACAG TTCGTCTTAC AGTGAGGATA GCGATGGCGA TCAGGATGAG 
AAAGCAGACA ACGGTGGTGC TGGTGGTGGC TCTAGTACGG ACACCAGTCT CTGTGATGAC 
GATGATCCCA AGTCAAGCGA AAAAAGCCCC AAGCGTAAGG CGAAGGTGGC ACGTAAGCTC 
AAAGAGAAGA GTCAGCATTT GGGGTCTCGG GAGTCCAGCG CAGAGCGTCA ACGACCGAAA 
TCCTGGGCAC CAGGCACGCA TGAGATACCC TTTATGTTGA TGGGCATGGA CAGCGGTGAT 
GAAAAGGAAG CCAAAAAGGA GGAGCAAGAC CTGGAGGAAG ACAGTGCCAC TGAAAGTGGT 
CGTTTTAGCT ATCCTAAGGA ACTAGATTGG ACTCAGGCGG CAGATAAAGT AGCAAGTTGT 
GAACTAGAAC CATTGGATAT TGACAAGATA CTGCCTAACA CTGCTATTTA TCTAAGCACA 
ATCAGTCTAA GTCCGCCCAA GACGGAAGAT GTGGGAGATC AGGATGTGAT AGAGGAACAA 
CCCATTGTGA AGCAGTTTGA CGTTAGTGAT TCTGGGGTAA CAAATCAATA TTTTGAGCGT 
GTCAACAGCG TTGAGCGTCC CAATAGGTTG GAGCTCTCAT ACTCTCTAAA CGAGGAGGAG 
GACTCTACAA TGTTAAAAGA CGATCGTCAA AGTTCGGATT TTGAATTGAA AATTGAACCC 
GAACCTTCCA CTTTAAATAA GGTCCCACAA ATTCGGGATG ATAACATACC CGGTGAAAAC 
AAAGATGACT ACAAGGAGCT TTTGAGCATG ACGATAGAAG AGCATAAGCC ACCAGCAGCT 
ACAATCACCA GCCAACTGAA TGCGAGTCGA AAGCGTCGGG ATCCCAGGCG TAAAACGCTA 
ACCAGATCCT CCACAATCGA GATTGAGGAA CGTTTTCAGG CCCTCGAACG AACACTGAGC 
CAAGATCAAC CGATTCATAA GTACATACCG AGCACTGCAG CTCTCGAAGA ACGCTATAAT 
AGTCTAGAAA AACAGTTGAG TGCCGAGAAA CAGCGGAAGG AGGAGGATCT GGATCGTATA 
CCTTCTACAG CTGATCTAGA ATCACGATTT GATGCCTTAA CGAAACAAAT GAGTTCCAGC 
GAATCGAGCA ATTCCAAGGC CCCACTCGAT CTTAATAAAG ATCAGCAGCA GCCTGGCGAG 
AAATCCAAAG AAACAGAAAG AAAATCAAAC AAAAACCAAA GCAAAGAAGG GGAGAAACAA 
ACCCAATCGG AACCTAAAGA AACTGCATCG GAAAAGGAAA GTCCTGGCCA AAAAGAAGAA 
ACCAAAGAAA TTGAAGAAGA ACAACAGCCA CGTCTCAAGA AACTGCCCTC TACAGCCGAA 
CTTGAGGATC GATTTAATGC TTTAGAACGA AAAATGAGCG TACAAAAATC TAGTTCGGCA 
AAGACAAAGA AAGAGCCACC CGATGAAGAA AAACCTGCCC AAAAAACTGA AGCTCAAACA 
AAGGAAACTA AATCTAAACA AGAATCAAGC CCACAAAAGA CTCCAGAAAA CATAGAGACA 
AGTGAAAAGA GCACGAAAAT CAGTAAGGAT TCAAATGAGG TTAAGGATAA AGCCGATGAG 
GTAAAACGAA AAGTTCCACC TTCAACCGAG GAGTTGGAAA AGCGTTTCAA TGCTTTGGAG 
AAACAACTTA GTACAACTAA TTTGGAAGCT GAGCAACCTA CTACTTCTAA GGCAAAGGAA 
ATCAAATCAG AATTACCCAA GCAAACTGAA AATGTAAAAG CGAACAAGCA AGAAATTCAA 
TCACAAAAAT CCATCAAGTC TTTCGATGAA AAAGTTAAAG TTATTAATAC AAAATTAACA 
GCCGATGAGC AGAAAATCCA AGACCACGAC AAATTGGCCG AAGAAAAACG TAAAAGTCTT 
GATGAGAAAA GTGAAAGAGC CAGTGGTCAG TTAAAAGAAA TGTTGCCTGA GAATGAAACC 
ATTAAATCAA AGACATCTCA ACCTGAAGTC GTCCGGGCTG ATGTTCAACC CAAAGAACTG 
GGTAAGCGAA GAGCTTCCGA ACCACCATCA ACAGAAGATT TGGAGAAACG CTATGAGACA 
CTTAAGCGTC GCATGAGTAG TAAAAATCAT TTTGGTAATC TAACAGAATC AGCAGATGAG 
TCCATGTCAA GGCGAGATCA AGAAGTAATA TCCGAGACTT TAGAGACTGG AGAGCCGGAG 
AGAAAACAAT CACCGCCGAC AACAGAATAT CTGGAAAGTA GGTATGAGAA GCTACATGGG 
GAAAACAAGA AAACAACGCC CAAGAAAGTG GATGTGGCGA TTGAGGCGCA TATATCACCA 
CCACCAGCTC CGCCACCACC TCCAAAGCCG AATATCCTAG CTGATCCGGC TATGCATAAG 
CAACGAGCTC TCATTGAAGA GCTACAGGCG AAAATTCAAG AGCAAGGACA GTCCACGCCT 
GGTGAAGAGA ATCTCAAGCC CAGCGAGATA AATCCACAGC GCAAACAGAG GCAAGAGCAA 
CAACGTCTGC TACAACGGCC CACGACAATG GGAGATGAGA CCTCGGAAGC ACCTGCAAAG 
ACCGCTTACT ACAGATCGGG AAATTACGAA CCCTGGCAGC AGCAGCACCA GCAGAAGCAG 
CGAATGGTTC GTCGTTTCTC TGATTTGCCA TCAAGAGCCG ATCTAGAAAA TCGCTTACAA 
TTCCTGGAGA AGCAATTGTA CAAGAAATTC TACAAGCAGC GCTGTGCAAG TGATTCCGAA 
GTTGCATCGC GGACGATCGA TGACGAAAAG CCCAGCACAT CGGCAGCCCG TGTGGCGGAA 
GTTCAGCTGG AGCAACGTGT CCTGGCCCTA GAGAAGCAAT TAAGTGAGAA TAGTCTAAAG 
CTGCTAGAGG CCATGCGTGT CAACGTGCCC AACGTCGATG GCGGCTCTCC GCGAAGACTA 
AGCACGGATA CGATAGATGC CACTGGCAAG GAACTGGTGC GGTATACACA GAACTTTGGT 
GAGCTGGACG AGTTGGGTGG AGATGGCTCA AATAAGCCCA TTAACATTAG TATCAATATC 
AAAATGATGC TCAACAAGGA ACAGCAACAA CAACGACAAC AACAACAGCA GCAGCAGCAG 
CAGGAGGAAA AGGCAGACAC TAGGACCAGC ACAGAGGATT TAAGGATGCG CTTGGAGCAA 
TTGGAACAGC AATTGATGGA AGAAAGAGCC AAGAACAATG GTTTTGTGGA TGTCGAAAGT 
GCTAATGATG GCGGAGCAGG AGAGGAGGCA AAGCAATTGG ATAAATCCGA AACTTCAGTA 
CCGGAAGAAC TAGAGGAAAA TAAAAAGCAA GAGAAGGACA GTCACAATCA AACGGTAAAA 
AGTGATGAAG CTGAAATGAC AGAAGATCCA ATTGTGGCCA CCACCGTGGA AACCATTGAG 
CCGGAGACAG TGAAAGAAAC AAAGACCTTA GAAAACGAGG AGATAGCTCA AGCAACCCAA 
GAGAAAGCTG CAAAAGCTGA GGAGATCGAA GAGACAACCA AAGATAAAAT AAATGAATCA 
TCTGAAGTCA TTCCTAAAGT GGAGGAAACT AAACCGCCAA CAGAAACAGA AACCCACTGC 
GAAGAGAAAA CTGTAGACTC TGACAAGATT ATTGTCAATG CCAGCTCTAT TGAACCTCCG 
GTGGATCCCA ATAATAAGAC CGTGGTCCTG CTTATGGATA ACGAACCTAG AGCTTCGAGA 
GTGCGCCGTT TGACCCGCGC CAATACCGAA GAACTGGAAG GTCTATTCCA GGCTTTAGAA 
AAACAACTGC AGGATCGCAA TCTAATCAAA TCTGAAGATG GGCGGTTAAT ACGAGCAAGT 
ACGGAACAGA AACAAACTGC AGAACAAGTA GAACAAGCTC AGGCTATAAG CGATCTAACT 
AAGGAAATCG AAGACTTTAC CAGCGGTAAA CCAGACGCAA AAAGTGAAGG CGAAACAAAG 
GAAGATAAGC CAGCAGATGG CGGCGAAGAA CCCCCCGACG ATTATGATTG GGGTCCCAAT 
CCGGTTAAGC ATCATTTGAA ACGCAAAACC GTATATTTGC CTTCAACCAA AGAGCTAGAG 
GCTCGTTTTC GGTCACTGGA ACGTCAAATT AAACTACTCG AAGATGTGGA GAAAATCGAT 
GTGGAGCAGC GCTTAAATGA AATTGAACGA AAGATTAAAC TGCAGTATTC CTTGTCACAT 
GAGAAAGATT TAAATAAATA TCTAGAGCTC TGCGAAGGCA AGGGCTTAGA TGAGGAAGAG 
GAGCCTATTG TAGATGCATC TCCTCCTTTA ACCAAGGATA CCCAGCGGTC CCGTAGTCCA 
GCACGTAAAG AAGCTTCAAA GTCGCCATAT ACTTCACCAT CGCGTAAGGC AGTTGCAAAA 
TCTCCTCATA CTTCTCCTGC ACGTAAGCCA GCTACAAAAT CTCCTTATAC CTCGCCTTCC 
CGTGCTAGTG ACAAAAAGCG GAGCCCTTAT ACCTCGCCAG TACGTCGACA GGCGCATCCC 
GGTGATTTAC CCATCTCCGA TGACTTGGAG TACAAGTATC GTGTGCTTGA TCTGGTACGT 
TCCAAATCCA AGGAGAACTT GTCGAAGCGT AAAACTGATC CCAATAAAAA GCCGCCCATT 
CATCCTCTTG AAATGCTACT TGATCCGAGT CCCGATGACA GCGAGATACC CACAACAGGG 
GAACTGGAGC ACCGCATACG TCTGCTGGAT GAAAAGCTAA AGTCGCCCTG CCGCCATAAA 
TCTCGCTCAC GTTCACCCAC CATCGATGAC ATGAAACGAA AGAAGCTCTT GGAGGAAAAG 
CGACCCAAAT CGCCGGTTCA TACATTGGAA CGTTTGGTCA GTTCACCTAA CAGACCAGAG 
CCACCCACTG AGGAGGAACT AGAACAGCGA ATGCGTGCCT TGGAAGACGA AAAGCGTTTC 
GACTTCAAGA CCCAAAAGGA TTACAAGGCA TTCAATCAAA AGCTTAAAGA TGTCATCTCA 
CCTTCCCTAT CTTTCGAAGA GTTCAAGGCG GCCAAATCGC GTGAACAGAG TCCACGGCGT 
CAAGGAGCCA CAACACCCAA ATCAGCAATG CGACGCGACG ATACAGATGA TTACCGAGGA 
CGCGAGGAGA CACCCCATTA TCGGCCCACT AGTCCTAAGG TGATACGCTT TCGAGATGAG 
GATGAGGATT ATTACAAGGA GAGCCAGCGT CCGAAGTCTC GACAGTCCAG CGAACGAATG 
AAGACTCTTG CAGATCGCAG CTACAGCAGC AGCATTGCAG CAGCAACGGC CAGCAGCTCC 
GAGGGTCTCG ATGCCCTGGG TAGTCGCCTA ATGAGAGAAA CCTCGCCAAT TACCCGCACT 
GGGACTCATA CTGGTGTGCC GTTACGGACT GGAGAGAATA TCAATGATCG TCTAAGTTCT 
ATCAAGGATA CCATCAAATC CATTGACACT CTATGCGAGG AGAAACCGTA TCAGAAGGAG 
AAATGCCAAC GTTATATTGA TTCCCTCTTC TCAGATTCAT TGCATTTCGG TTGTAAGAAG 
AATTCCATGG AGGAGCTAAC GCACAGTCGC AGCGAGAGTC GAGGACGTAC AGCACAACGA 
TCGAGTGACT ATACGCCGGC CATTCGAGTC ACCTCAGAGC ATCGATCTCT GGGCTCGGCC 
GACTCGAAGC GCACAAGTCC ATTGCGGGCA TCCAGTCCGT TGCATCATCA TCGCTCGCAT 
AGGGATATCA GTAGGGAGTT ATCGCCGCGC AGGCGTCGTG ATGCCGAGGA TATGGAGGAG 
CGCGAAAGTA GTAGGGTAAG ACGTGATAAT ATGTTGCCAA ATTATTTTCT CGATAATCGT 
AGCGATTTAA GTAGCGGCAG TAGTTTAACT AAGTTTCATA AAGTAGATAG ACAACTAGAA 
GAAACGTGCG CCAAGTACGA AGCTGACGAT CGACGCTCGG CCAGCAGAAC ACCATTGAGT 
TCGCCATATG AGTCCAGAAC AACAGCCACA CGCTACAACA ACAACAACAA CACAGGCACA 
GGGACAACAA CAACAACAAC CGCCGATACA TTTCAAAGGC CCGTATCGCC CTATCGCCAG 
CCCTATGACC CATATAGACG TAATGTGAAT GCCCCATCCC CGGTTCCGGT TTATCAGCCA 
GCAAAGCTGG AAATTCGTCA TACGACAGTC ACATCAACCT TCTATGATCG TTTTCTCACC 
GAAAAACAAA TTGAGAAGCA AACACTCTCA CGTCCACCCA GTCGTTCGCC TGTAGTCTCA 
CCATCGGCCA GCATAGCCAA AAGCGATACT GATCTGCCGG GCACAGTGAG CAGTGCCTCT 
GCTTCCGCTT CCGCCTCACA ATTCGCTTCC TCCTACTCCT CCGCCGCCTA CAAGCCCACA 
GTAACCACCG CAATCATGTC CAGCAGTTAT GCTGGTACTG CCACCTCACA TTTTACTTCT 
TCGTTGCCCA CAAGCAATTA TTCATATCTC ATGAGCACTT CCACCAACGC CACCACCACC 
ACCACCTCTT CCTCCTCCAC TACTGGTGGT TCGTCTGGGG GATATGTGCC ATATAATTTC 
AGCAGCTCGT TTAGCAATCG TCATAGTGAT CCAATTGCCA ATTCTTCTGG CATCTCGAGC 
GGTAGCACTG GTGTCTATAA TCCCATGATG TCGTTCACTT TGCGTGAACC AACAAGTGCC 
TCGGGCAGTC TCTTGGGTCA ATATGCCTAC AAAAGTAGTT TCGTCTCCAA CTACAGTTCG 
TCATCCAATC AAATGCCCAA GTCACCATCC ACCAGTGATC AGGACAAGCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_023179840.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023035608.1 Drosophila willistoni titin (LOC6649543), transcript variant X3, mRNA.

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CloneID	ODf27215
Clone ID Related Accession (Same CDS sequence)	XM_023179840.1
Accession Version	XM_023179840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	uncharacterized protein LOC6649543 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032872.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATG CACAGCCTAA AATGCGTTTT AGCCTTTCAC CACCGCCGCA GAAAGCTATT 
ACCGCCAGCG TAACCTCAAC CAAGAGTAGC ACAAACATTG ACATTAGCTC CAGCTCCAGC 
ACCATCACCA CCGCGGCTAC AACCAGCACC ACCAAAACAA CAACAACCAC TTTCTCCACC 
ACCACCACCA ACAACAAAAA TAATATGCTG GGTCATGAGG GGAACAGCTC AAATTCAGCT 
GCCTCTATTT CTGCATCAGC TGACGAAGAT GCCGCCTCGG ATTATAATCA ATGGCTGCAT 
GCCATGAAAC TGGTGGCTCG TCTACCAGGA GGCACACCAC CCGAGTTTCG TCGCAAGCTC 
TGGCTCTCGT TGGCTGACAA ATATCTCAAG TCCAAGAATG TTGATTGGAC GCAGCAGCGT 
GAGAAATGCT TTTGCGAGGA ATGGCGTGAA GATGACGAAG AGCTGGGCAT ACAAATTGTC 
AAGGATTTGC ATCGCACTGG TTCTAATTTG TGCACTGGTC CCGCGGGCTC CATTAACCAG 
GCCAAGCTGA AGCGCATTCT ACTCGGCTAT GCCCGCTATA ATCCCGAGGT GGGCTATTGT 
CAGGGCTTCA ATATGCTGGG TGCTTTGATT CTACAAGTCA TGGACAAGGA AGAGGAGGAG 
TCCATGAAGG TCATGATCTA TCTGGTGGAG GGTGTACTGC CAACGGGTTA TTTCTATGGT 
TCAATGGGCG GTCTTCAGGC GGATATGGGA GTCTTTCGCG AGCTTATGCA AACGAAACTG 
CCGCGTCTGG CCAAGCATTT GCAGCGTTTG CAGGGAGGAC CCGTTGAGAA TGCCTACGAA 
CCGCCATTGA CCAATGTCTT CACCATGCAA TGGTTCCTCA CCATGTTCTG CACCTGCCTA 
CCCATGTCCT GTGTCCTGCG CGTCTGGGAT CTCGTTCTCA TCGAAGGCAG TGATGTTCTA 
TTGCGCACTG CCCTCGTCCT GTGGAGTCTA TTGGAAGAAC GTGTCCTTAA TGCTCGCACA 
GCTGATGAGT TCTATGGCAA AATGGGATCT TTCTCGAGTG AATTGCTCAA TGGTCATCTT 
ATCGATTCAA ATGGTCTAAT CGAAAAGGTT GTTAAACTCG GACCCATTGC CGATTTGCGT 
CATTTGAGGG ATAAACATCT ATATAACATA GCCCCATTGA GGCATAAACA AGGCATACAA 
CTCTATTACG ATGACGATGA TACACATTCG GATGAGGAGC GCATGGCAGT GGCTACCGTG 
TGGGGTTTAA ATTGGGGTCG CCGCGGTTCG GTGGGACCCG GAGCCCCAGC GGGTAGGCAG 
CCATCTGAAC AAAAGGATCG TCTGGCCTTG GATATATCGT TGCTGAAGAA GCAATACGAT 
CGTCTTCGAG AACGTCAGAA GCAGGCCCAT GTCATCCTAA CTACTGCATG CTCAACAGCA 
ACTCGTCAAA CGCCTTCAGC GCAAAGCTCG GTGCCGGTTA ATCAGTTACT ACAGGGTCGA 
CACGCCATTG TGACCAACAG GGGCAAACGG GTGAGTGCTC CCTTGGGAGC CATACCTCCA 
GCCCGAAAGC CTTCACTGCC AGCTGTATTG CACGGAAACG GAAAGCCTAT GATGGGGGAG 
AAGCAGTTGC GACGAGGAGA AACCCTACTC TGGCGGGACA CGGACACTAG TCGACGGCGA 
AGAGACAGCC TCACGTGGAA GGAGATCAAA GCAGATCGTG CGGCCATGCT ACGCGAAGGA 
GTTGATTCAA GCTCTCTAAA GACCCAGAAA CTTCGCACGC GGTTGGGCAA AAGTGACAGT 
TCGTCTTACA GTGAGGATAG CGATGGCGAT CAGGATGAGA AAGCAGACAA CGGTGGTGCT 
GGTGGTGGCT CTAGTACGGA CACCAGTCTC TGTGATGACG ATGATCCCAA GTCAAGCGAA 
AAAAGCCCCA AGCGTAAGGC GAAGGTGGCA CGTAAGCTCA AAGAGAAGAG TCAGCATTTG 
GGGTCTCGGG AGTCCAGCGC AGAGCGTCAA CGACCGAAAT CCTGGGCACC AGGCACGCAT 
GAGATACCCT TTATGTTGAT GGGCATGGAC AGCGGTGATG AAAAGGAAGC CAAAAAGGAG 
GAGCAAGACC TGGAGGAAGA CAGTGCCACT GAAAGTGGTC GTTTTAGCTA TCCTAAGGAA 
CTAGATTGGA CTCAGGCGGC AGATAAAGTA GCAAGTTGTG AACTAGAACC ATTGGATATT 
GACAAGATAC TGCCTAACAC TGCTATTTAT CTAAGCACAA TCAGTCTAAG TCCGCCCAAG 
ACGGAAGATG TGGGAGATCA GGATGTGATA GAGGAACAAC CCATTGTGAA GCAGTTTGAC 
GTTAGTGATT CTGGGGTAAC AAATCAATAT TTTGAGCGTG TCAACAGCGT TGAGCGTCCC 
AATAGGTTGG AGCTCTCATA CTCTCTAAAC GAGGAGGAGG ACTCTACAAT GTTAAAAGAC 
GATCGTCAAA GTTCGGATTT TGAATTGAAA ATTGAACCCG AACCTTCCAC TTTAAATAAG 
GTCCCACAAA TTCGGGATGA TAACATACCC GGTGAAAACA AAGATGACTA CAAGGAGCTT 
TTGAGCATGA CGATAGAAGA GCATAAGCCA CCAGCAGCTA CAATCACCAG CCAACTGAAT 
GCGAGTCGAA AGCGTCGGGA TCCCAGGCGT AAAACGCTAA CCAGATCCTC CACAATCGAG 
ATTGAGGAAC GTTTTCAGGC CCTCGAACGA ACACTGAGCC AAGATCAACC GATTCATAAG 
TACATACCGA GCACTGCAGC TCTCGAAGAA CGCTATAATA GTCTAGAAAA ACAGTTGAGT 
GCCGAGAAAC AGCGGAAGGA GGAGGATCTG GATCGTATAC CTTCTACAGC TGATCTAGAA 
TCACGATTTG ATGCCTTAAC GAAACAAATG AGTTCCAGCG AATCGAGCAA TTCCAAGGCC 
CCACTCGATC TTAATAAAGA TCAGCAGCAG CCTGGCGAGA AATCCAAAGA AACAGAAAGA 
AAATCAAACA AAAACCAAAG CAAAGAAGGG GAGAAACAAA CCCAATCGGA ACCTAAAGAA 
ACTGCATCGG AAAAGGAAAG TCCTGGCCAA AAAGAAGAAA CCAAAGAAAT TGAAGAAGAA 
CAACAGCCAC GTCTCAAGAA ACTGCCCTCT ACAGCCGAAC TTGAGGATCG ATTTAATGCT 
TTAGAACGAA AAATGAGCGT ACAAAAATCT AGTTCGGCAA AGACAAAGAA AGAGCCACCC 
GATGAAGAAA AACCTGCCCA AAAAACTGAA GCTCAAACAA AGGAAACTAA ATCTAAACAA 
GAATCAAGCC CACAAAAGAC TCCAGAAAAC ATAGAGACAA GTGAAAAGAG CACGAAAATC 
AGTAAGGATT CAAATGAGGT TAAGGATAAA GCCGATGAGG TAAAACGAAA AGTTCCACCT 
TCAACCGAGG AGTTGGAAAA GCGTTTCAAT GCTTTGGAGA AACAACTTAG TACAACTAAT 
TTGGAAGCTG AGCAACCTAC TACTTCTAAG GCAAAGGAAA TCAAATCAGA ATTACCCAAG 
CAAACTGAAA ATGTAAAAGC GAACAAGCAA GAAATTCAAT CACAAAAATC CATCAAGTCT 
TTCGATGAAA AAGTTAAAGT TATTAATACA AAATTAACAG CCGATGAGCA GAAAATCCAA 
GACCACGACA AATTGGCCGA AGAAAAACGT AAAAGTCTTG ATGAGAAAAG TGAAAGAGCC 
AGTGGTCAGT TAAAAGAAAT GTTGCCTGAG AATGAAACCA TTAAATCAAA GACATCTCAA 
CCTGAAGTCG TCCGGGCTGA TGTTCAACCC AAAGAACTGG GTAAGCGAAG AGCTTCCGAA 
CCACCATCAA CAGAAGATTT GGAGAAACGC TATGAGACAC TTAAGCGTCG CATGAGTAGT 
AAAAATCATT TTGGTAATCT AACAGAATCA GCAGATGAGT CCATGTCAAG GCGAGATCAA 
GAAGTAATAT CCGAGACTTT AGAGACTGGA GAGCCGGAGA GAAAACAATC ACCGCCGACA 
ACAGAATATC TGGAAAGTAG GTATGAGAAG CTACATGGGG AAAACAAGAA AACAACGCCC 
AAGAAAGTGG ATGTGGCGAT TGAGGCGCAT ATATCACCAC CACCAGCTCC GCCACCACCT 
CCAAAGCCGA ATATCCTAGC TGATCCGGCT ATGCATAAGC AACGAGCTCT CATTGAAGAG 
CTACAGGCGA AAATTCAAGA GCAAGGACAG TCCACGCCTG GTGAAGAGAA TCTCAAGCCC 
AGCGAGATAA ATCCACAGCG CAAACAGAGG CAAGAGCAAC AACGTCTGCT ACAACGGCCC 
ACGACAATGG GAGATGAGAC CTCGGAAGCA CCTGCAAAGA CCGCTTACTA CAGATCGGGA 
AATTACGAAC CCTGGCAGCA GCAGCACCAG CAGAAGCAGC GAATGGTTCG TCGTTTCTCT 
GATTTGCCAT CAAGAGCCGA TCTAGAAAAT CGCTTACAAT TCCTGGAGAA GCAATTGTAC 
AAGAAATTCT ACAAGCAGCG CTGTGCAAGT GATTCCGAAG TTGCATCGCG GACGATCGAT 
GACGAAAAGC CCAGCACATC GGCAGCCCGT GTGGCGGAAG TTCAGCTGGA GCAACGTGTC 
CTGGCCCTAG AGAAGCAATT AAGTGAGAAT AGTCTAAAGC TGCTAGAGGC CATGCGTGTC 
AACGTGCCCA ACGTCGATGG CGGCTCTCCG CGAAGACTAA GCACGGATAC GATAGATGCC 
ACTGGCAAGG AACTGGTGCG GTATACACAG AACTTTGGTG AGCTGGACGA GTTGGGTGGA 
GATGGCTCAA ATAAGCCCAT TAACATTAGT ATCAATATCA AAATGATGCT CAACAAGGAA 
CAGCAACAAC AACGACAACA ACAACAGCAG CAGCAGCAGC AGGAGGAAAA GGCAGACACT 
AGGACCAGCA CAGAGGATTT AAGGATGCGC TTGGAGCAAT TGGAACAGCA ATTGATGGAA 
GAAAGAGCCA AGAACAATGG TTTTGTGGAT GTCGAAAGTG CTAATGATGG CGGAGCAGGA 
GAGGAGGCAA AGCAATTGGA TAAATCCGAA ACTTCAGTAC CGGAAGAACT AGAGGAAAAT 
AAAAAGCAAG AGAAGGACAG TCACAATCAA ACGGTAAAAA GTGATGAAGC TGAAATGACA 
GAAGATCCAA TTGTGGCCAC CACCGTGGAA ACCATTGAGC CGGAGACAGT GAAAGAAACA 
AAGACCTTAG AAAACGAGGA GATAGCTCAA GCAACCCAAG AGAAAGCTGC AAAAGCTGAG 
GAGATCGAAG AGACAACCAA AGATAAAATA AATGAATCAT CTGAAGTCAT TCCTAAAGTG 
GAGGAAACTA AACCGCCAAC AGAAACAGAA ACCCACTGCG AAGAGAAAAC TGTAGACTCT 
GACAAGATTA TTGTCAATGC CAGCTCTATT GAACCTCCGG TGGATCCCAA TAATAAGACC 
GTGGTCCTGC TTATGGATAA CGAACCTAGA GCTTCGAGAG TGCGCCGTTT GACCCGCGCC 
AATACCGAAG AACTGGAAGG TCTATTCCAG GCTTTAGAAA AACAACTGCA GGATCGCAAT 
CTAATCAAAT CTGAAGATGG GCGGTTAATA CGAGCAAGTA CGGAACAGAA ACAAACTGCA 
GAACAAGTAG AACAAGCTCA GGCTATAAGC GATCTAACTA AGGAAATCGA AGACTTTACC 
AGCGGTAAAC CAGACGCAAA AAGTGAAGGC GAAACAAAGG AAGATAAGCC AGCAGATGGC 
GGCGAAGAAC CCCCCGACGA TTATGATTGG GGTCCCAATC CGGTTAAGCA TCATTTGAAA 
CGCAAAACCG TATATTTGCC TTCAACCAAA GAGCTAGAGG CTCGTTTTCG GTCACTGGAA 
CGTCAAATTA AACTACTCGA AGATGTGGAG AAAATCGATG TGGAGCAGCG CTTAAATGAA 
ATTGAACGAA AGATTAAACT GCAGTATTCC TTGTCACATG AGAAAGATTT AAATAAATAT 
CTAGAGCTCT GCGAAGGCAA GGGCTTAGAT GAGGAAGAGG AGCCTATTGT AGATGCATCT 
CCTCCTTTAA CCAAGGATAC CCAGCGGTCC CGTAGTCCAG CACGTAAAGA AGCTTCAAAG 
TCGCCATATA CTTCACCATC GCGTAAGGCA GTTGCAAAAT CTCCTCATAC TTCTCCTGCA 
CGTAAGCCAG CTACAAAATC TCCTTATACC TCGCCTTCCC GTGCTAGTGA CAAAAAGCGG 
AGCCCTTATA CCTCGCCAGT ACGTCGACAG GCGCATCCCG GTGATTTACC CATCTCCGAT 
GACTTGGAGT ACAAGTATCG TGTGCTTGAT CTGGTACGTT CCAAATCCAA GGAGAACTTG 
TCGAAGCGTA AAACTGATCC CAATAAAAAG CCGCCCATTC ATCCTCTTGA AATGCTACTT 
GATCCGAGTC CCGATGACAG CGAGATACCC ACAACAGGGG AACTGGAGCA CCGCATACGT 
CTGCTGGATG AAAAGCTAAA GTCGCCCTGC CGCCATAAAT CTCGCTCACG TTCACCCACC 
ATCGATGACA TGAAACGAAA GAAGCTCTTG GAGGAAAAGC GACCCAAATC GCCGGTTCAT 
ACATTGGAAC GTTTGGTCAG TTCACCTAAC AGACCAGAGC CACCCACTGA GGAGGAACTA 
GAACAGCGAA TGCGTGCCTT GGAAGACGAA AAGCGTTTCG ACTTCAAGAC CCAAAAGGAT 
TACAAGGCAT TCAATCAAAA GCTTAAAGAT GTCATCTCAC CTTCCCTATC TTTCGAAGAG 
TTCAAGGCGG CCAAATCGCG TGAACAGAGT CCACGGCGTC AAGGAGCCAC AACACCCAAA 
TCAGCAATGC GACGCGACGA TACAGATGAT TACCGAGGAC GCGAGGAGAC ACCCCATTAT 
CGGCCCACTA GTCCTAAGGT GATACGCTTT CGAGATGAGG ATGAGGATTA TTACAAGGAG 
AGCCAGCGTC CGAAGTCTCG ACAGTCCAGC GAACGAATGA AGACTCTTGC AGATCGCAGC 
TACAGCAGCA GCATTGCAGC AGCAACGGCC AGCAGCTCCG AGGGTCTCGA TGCCCTGGGT 
AGTCGCCTAA TGAGAGAAAC CTCGCCAATT ACCCGCACTG GGACTCATAC TGGTGTGCCG 
TTACGGACTG GAGAGAATAT CAATGATCGT CTAAGTTCTA TCAAGGATAC CATCAAATCC 
ATTGACACTC TATGCGAGGA GAAACCGTAT CAGAAGGAGA AATGCCAACG TTATATTGAT 
TCCCTCTTCT CAGATTCATT GCATTTCGGT TGTAAGAAGA ATTCCATGGA GGAGCTAACG 
CACAGTCGCA GCGAGAGTCG AGGACGTACA GCACAACGAT CGAGTGACTA TACGCCGGCC 
ATTCGAGTCA CCTCAGAGCA TCGATCTCTG GGCTCGGCCG ACTCGAAGCG CACAAGTCCA 
TTGCGGGCAT CCAGTCCGTT GCATCATCAT CGCTCGCATA GGGATATCAG TAGGGAGTTA 
TCGCCGCGCA GGCGTCGTGA TGCCGAGGAT ATGGAGGAGC GCGAAAGTAG TAGGGTAAGA 
CGTGATAATA TGTTGCCAAA TTATTTTCTC GATAATCGTA GCGATTTAAG TAGCGGCAGT 
AGTTTAACTA AGTTTCATAA AGTAGATAGA CAACTAGAAG AAACGTGCGC CAAGTACGAA 
GCTGACGATC GACGCTCGGC CAGCAGAACA CCATTGAGTT CGCCATATGA GTCCAGAACA 
ACAGCCACAC GCTACAACAA CAACAACAAC ACAGGCACAG GGACAACAAC AACAACAACC 
GCCGATACAT TTCAAAGGCC CGTATCGCCC TATCGCCAGC CCTATGACCC ATATAGACGT 
AATGTGAATG CCCCATCCCC GGTTCCGGTT TATCAGCCAG CAAAGCTGGA AATTCGTCAT 
ACGACAGTCA CATCAACCTT CTATGATCGT TTTCTCACCG AAAAACAAAT TGAGAAGCAA 
ACACTCTCAC GTCCACCCAG TCGTTCGCCT GTAGTCTCAC CATCGGCCAG CATAGCCAAA 
AGCGATACTG ATCTGCCGGG CACAGTGAGC AGTGCCTCTG CTTCCGCTTC CGCCTCACAA 
TTCGCTTCCT CCTACTCCTC CGCCGCCTAC AAGCCCACAG TAACCACCGC AATCATGTCC 
AGCAGTTATG CTGGTACTGC CACCTCACAT TTTACTTCTT CGTTGCCCAC AAGCAATTAT 
TCATATCTCA TGAGCACTTC CACCAACGCC ACCACCACCA CCACCTCTTC CTCCTCCACT 
ACTGGTGGTT CGTCTGGGGG ATATGTGCCA TATAATTTCA GCAGCTCGTT TAGCAATCGT 
CATAGTGATC CAATTGCCAA TTCTTCTGGC ATCTCGAGCG GTAGCACTGG TGTCTATAAT 
CCCATGATGT CGTTCACTTT GCGTGAACCA ACAAGTGCCT CGGGCAGTCT CTTGGGTCAA 
TATGCCTACA AAAGTAGTTT CGTCTCCAAC TACAGTTCGT CATCCAATCA AATGCCCAAG 
TCACCATCCA CCAGTGATCA GGACAAGCTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023035608.1
CDS	76..8628
Translation	MDDAQPKMRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMKTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_023179840.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni titin (LOC6649543), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023179840.1

ORF Insert Sequence:

ATGGACGATG CACAGCCTAA AATGCGTTTT AGCCTTTCAC CACCGCCGCA GAAAGCTATT 
ACCGCCAGCG TAACCTCAAC CAAGAGTAGC ACAAACATTG ACATTAGCTC CAGCTCCAGC 
ACCATCACCA CCGCGGCTAC AACCAGCACC ACCAAAACAA CAACAACCAC TTTCTCCACC 
ACCACCACCA ACAACAAAAA TAATATGCTG GGTCATGAGG GGAACAGCTC AAATTCAGCT 
GCCTCTATTT CTGCATCAGC TGACGAAGAT GCCGCCTCGG ATTATAATCA ATGGCTGCAT 
GCCATGAAAC TGGTGGCTCG TCTACCAGGA GGCACACCAC CCGAGTTTCG TCGCAAGCTC 
TGGCTCTCGT TGGCTGACAA ATATCTCAAG TCCAAGAATG TTGATTGGAC GCAGCAGCGT 
GAGAAATGCT TTTGCGAGGA ATGGCGTGAA GATGACGAAG AGCTGGGCAT ACAAATTGTC 
AAGGATTTGC ATCGCACTGG TTCTAATTTG TGCACTGGTC CCGCGGGCTC CATTAACCAG 
GCCAAGCTGA AGCGCATTCT ACTCGGCTAT GCCCGCTATA ATCCCGAGGT GGGCTATTGT 
CAGGGCTTCA ATATGCTGGG TGCTTTGATT CTACAAGTCA TGGACAAGGA AGAGGAGGAG 
TCCATGAAGG TCATGATCTA TCTGGTGGAG GGTGTACTGC CAACGGGTTA TTTCTATGGT 
TCAATGGGCG GTCTTCAGGC GGATATGGGA GTCTTTCGCG AGCTTATGCA AACGAAACTG 
CCGCGTCTGG CCAAGCATTT GCAGCGTTTG CAGGGAGGAC CCGTTGAGAA TGCCTACGAA 
CCGCCATTGA CCAATGTCTT CACCATGCAA TGGTTCCTCA CCATGTTCTG CACCTGCCTA 
CCCATGTCCT GTGTCCTGCG CGTCTGGGAT CTCGTTCTCA TCGAAGGCAG TGATGTTCTA 
TTGCGCACTG CCCTCGTCCT GTGGAGTCTA TTGGAAGAAC GTGTCCTTAA TGCTCGCACA 
GCTGATGAGT TCTATGGCAA AATGGGATCT TTCTCGAGTG AATTGCTCAA TGGTCATCTT 
ATCGATTCAA ATGGTCTAAT CGAAAAGGTT GTTAAACTCG GACCCATTGC CGATTTGCGT 
CATTTGAGGG ATAAACATCT ATATAACATA GCCCCATTGA GGCATAAACA AGGCATACAA 
CTCTATTACG ATGACGATGA TACACATTCG GATGAGGAGC GCATGGCAGT GGCTACCGTG 
TGGGGTTTAA ATTGGGGTCG CCGCGGTTCG GTGGGACCCG GAGCCCCAGC GGGTAGGCAG 
CCATCTGAAC AAAAGGATCG TCTGGCCTTG GATATATCGT TGCTGAAGAA GCAATACGAT 
CGTCTTCGAG AACGTCAGAA GCAGGCCCAT GTCATCCTAA CTACTGCATG CTCAACAGCA 
ACTCGTCAAA CGCCTTCAGC GCAAAGCTCG GTGCCGGTTA ATCAGTTACT ACAGGGTCGA 
CACGCCATTG TGACCAACAG GGGCAAACGG GTGAGTGCTC CCTTGGGAGC CATACCTCCA 
GCCCGAAAGC CTTCACTGCC AGCTGTATTG CACGGAAACG GAAAGCCTAT GATGGGGGAG 
AAGCAGTTGC GACGAGGAGA AACCCTACTC TGGCGGGACA CGGACACTAG TCGACGGCGA 
AGAGACAGCC TCACGTGGAA GGAGATCAAA GCAGATCGTG CGGCCATGCT ACGCGAAGGA 
GTTGATTCAA GCTCTCTAAA GACCCAGAAA CTTCGCACGC GGTTGGGCAA AAGTGACAGT 
TCGTCTTACA GTGAGGATAG CGATGGCGAT CAGGATGAGA AAGCAGACAA CGGTGGTGCT 
GGTGGTGGCT CTAGTACGGA CACCAGTCTC TGTGATGACG ATGATCCCAA GTCAAGCGAA 
AAAAGCCCCA AGCGTAAGGC GAAGGTGGCA CGTAAGCTCA AAGAGAAGAG TCAGCATTTG 
GGGTCTCGGG AGTCCAGCGC AGAGCGTCAA CGACCGAAAT CCTGGGCACC AGGCACGCAT 
GAGATACCCT TTATGTTGAT GGGCATGGAC AGCGGTGATG AAAAGGAAGC CAAAAAGGAG 
GAGCAAGACC TGGAGGAAGA CAGTGCCACT GAAAGTGGTC GTTTTAGCTA TCCTAAGGAA 
CTAGATTGGA CTCAGGCGGC AGATAAAGTA GCAAGTTGTG AACTAGAACC ATTGGATATT 
GACAAGATAC TGCCTAACAC TGCTATTTAT CTAAGCACAA TCAGTCTAAG TCCGCCCAAG 
ACGGAAGATG TGGGAGATCA GGATGTGATA GAGGAACAAC CCATTGTGAA GCAGTTTGAC 
GTTAGTGATT CTGGGGTAAC AAATCAATAT TTTGAGCGTG TCAACAGCGT TGAGCGTCCC 
AATAGGTTGG AGCTCTCATA CTCTCTAAAC GAGGAGGAGG ACTCTACAAT GTTAAAAGAC 
GATCGTCAAA GTTCGGATTT TGAATTGAAA ATTGAACCCG AACCTTCCAC TTTAAATAAG 
GTCCCACAAA TTCGGGATGA TAACATACCC GGTGAAAACA AAGATGACTA CAAGGAGCTT 
TTGAGCATGA CGATAGAAGA GCATAAGCCA CCAGCAGCTA CAATCACCAG CCAACTGAAT 
GCGAGTCGAA AGCGTCGGGA TCCCAGGCGT AAAACGCTAA CCAGATCCTC CACAATCGAG 
ATTGAGGAAC GTTTTCAGGC CCTCGAACGA ACACTGAGCC AAGATCAACC GATTCATAAG 
TACATACCGA GCACTGCAGC TCTCGAAGAA CGCTATAATA GTCTAGAAAA ACAGTTGAGT 
GCCGAGAAAC AGCGGAAGGA GGAGGATCTG GATCGTATAC CTTCTACAGC TGATCTAGAA 
TCACGATTTG ATGCCTTAAC GAAACAAATG AGTTCCAGCG AATCGAGCAA TTCCAAGGCC 
CCACTCGATC TTAATAAAGA TCAGCAGCAG CCTGGCGAGA AATCCAAAGA AACAGAAAGA 
AAATCAAACA AAAACCAAAG CAAAGAAGGG GAGAAACAAA CCCAATCGGA ACCTAAAGAA 
ACTGCATCGG AAAAGGAAAG TCCTGGCCAA AAAGAAGAAA CCAAAGAAAT TGAAGAAGAA 
CAACAGCCAC GTCTCAAGAA ACTGCCCTCT ACAGCCGAAC TTGAGGATCG ATTTAATGCT 
TTAGAACGAA AAATGAGCGT ACAAAAATCT AGTTCGGCAA AGACAAAGAA AGAGCCACCC 
GATGAAGAAA AACCTGCCCA AAAAACTGAA GCTCAAACAA AGGAAACTAA ATCTAAACAA 
GAATCAAGCC CACAAAAGAC TCCAGAAAAC ATAGAGACAA GTGAAAAGAG CACGAAAATC 
AGTAAGGATT CAAATGAGGT TAAGGATAAA GCCGATGAGG TAAAACGAAA AGTTCCACCT 
TCAACCGAGG AGTTGGAAAA GCGTTTCAAT GCTTTGGAGA AACAACTTAG TACAACTAAT 
TTGGAAGCTG AGCAACCTAC TACTTCTAAG GCAAAGGAAA TCAAATCAGA ATTACCCAAG 
CAAACTGAAA ATGTAAAAGC GAACAAGCAA GAAATTCAAT CACAAAAATC CATCAAGTCT 
TTCGATGAAA AAGTTAAAGT TATTAATACA AAATTAACAG CCGATGAGCA GAAAATCCAA 
GACCACGACA AATTGGCCGA AGAAAAACGT AAAAGTCTTG ATGAGAAAAG TGAAAGAGCC 
AGTGGTCAGT TAAAAGAAAT GTTGCCTGAG AATGAAACCA TTAAATCAAA GACATCTCAA 
CCTGAAGTCG TCCGGGCTGA TGTTCAACCC AAAGAACTGG GTAAGCGAAG AGCTTCCGAA 
CCACCATCAA CAGAAGATTT GGAGAAACGC TATGAGACAC TTAAGCGTCG CATGAGTAGT 
AAAAATCATT TTGGTAATCT AACAGAATCA GCAGATGAGT CCATGTCAAG GCGAGATCAA 
GAAGTAATAT CCGAGACTTT AGAGACTGGA GAGCCGGAGA GAAAACAATC ACCGCCGACA 
ACAGAATATC TGGAAAGTAG GTATGAGAAG CTACATGGGG AAAACAAGAA AACAACGCCC 
AAGAAAGTGG ATGTGGCGAT TGAGGCGCAT ATATCACCAC CACCAGCTCC GCCACCACCT 
CCAAAGCCGA ATATCCTAGC TGATCCGGCT ATGCATAAGC AACGAGCTCT CATTGAAGAG 
CTACAGGCGA AAATTCAAGA GCAAGGACAG TCCACGCCTG GTGAAGAGAA TCTCAAGCCC 
AGCGAGATAA ATCCACAGCG CAAACAGAGG CAAGAGCAAC AACGTCTGCT ACAACGGCCC 
ACGACAATGG GAGATGAGAC CTCGGAAGCA CCTGCAAAGA CCGCTTACTA CAGATCGGGA 
AATTACGAAC CCTGGCAGCA GCAGCACCAG CAGAAGCAGC GAATGGTTCG TCGTTTCTCT 
GATTTGCCAT CAAGAGCCGA TCTAGAAAAT CGCTTACAAT TCCTGGAGAA GCAATTGTAC 
AAGAAATTCT ACAAGCAGCG CTGTGCAAGT GATTCCGAAG TTGCATCGCG GACGATCGAT 
GACGAAAAGC CCAGCACATC GGCAGCCCGT GTGGCGGAAG TTCAGCTGGA GCAACGTGTC 
CTGGCCCTAG AGAAGCAATT AAGTGAGAAT AGTCTAAAGC TGCTAGAGGC CATGCGTGTC 
AACGTGCCCA ACGTCGATGG CGGCTCTCCG CGAAGACTAA GCACGGATAC GATAGATGCC 
ACTGGCAAGG AACTGGTGCG GTATACACAG AACTTTGGTG AGCTGGACGA GTTGGGTGGA 
GATGGCTCAA ATAAGCCCAT TAACATTAGT ATCAATATCA AAATGATGCT CAACAAGGAA 
CAGCAACAAC AACGACAACA ACAACAGCAG CAGCAGCAGC AGGAGGAAAA GGCAGACACT 
AGGACCAGCA CAGAGGATTT AAGGATGCGC TTGGAGCAAT TGGAACAGCA ATTGATGGAA 
GAAAGAGCCA AGAACAATGG TTTTGTGGAT GTCGAAAGTG CTAATGATGG CGGAGCAGGA 
GAGGAGGCAA AGCAATTGGA TAAATCCGAA ACTTCAGTAC CGGAAGAACT AGAGGAAAAT 
AAAAAGCAAG AGAAGGACAG TCACAATCAA ACGGTAAAAA GTGATGAAGC TGAAATGACA 
GAAGATCCAA TTGTGGCCAC CACCGTGGAA ACCATTGAGC CGGAGACAGT GAAAGAAACA 
AAGACCTTAG AAAACGAGGA GATAGCTCAA GCAACCCAAG AGAAAGCTGC AAAAGCTGAG 
GAGATCGAAG AGACAACCAA AGATAAAATA AATGAATCAT CTGAAGTCAT TCCTAAAGTG 
GAGGAAACTA AACCGCCAAC AGAAACAGAA ACCCACTGCG AAGAGAAAAC TGTAGACTCT 
GACAAGATTA TTGTCAATGC CAGCTCTATT GAACCTCCGG TGGATCCCAA TAATAAGACC 
GTGGTCCTGC TTATGGATAA CGAACCTAGA GCTTCGAGAG TGCGCCGTTT GACCCGCGCC 
AATACCGAAG AACTGGAAGG TCTATTCCAG GCTTTAGAAA AACAACTGCA GGATCGCAAT 
CTAATCAAAT CTGAAGATGG GCGGTTAATA CGAGCAAGTA CGGAACAGAA ACAAACTGCA 
GAACAAGTAG AACAAGCTCA GGCTATAAGC GATCTAACTA AGGAAATCGA AGACTTTACC 
AGCGGTAAAC CAGACGCAAA AAGTGAAGGC GAAACAAAGG AAGATAAGCC AGCAGATGGC 
GGCGAAGAAC CCCCCGACGA TTATGATTGG GGTCCCAATC CGGTTAAGCA TCATTTGAAA 
CGCAAAACCG TATATTTGCC TTCAACCAAA GAGCTAGAGG CTCGTTTTCG GTCACTGGAA 
CGTCAAATTA AACTACTCGA AGATGTGGAG AAAATCGATG TGGAGCAGCG CTTAAATGAA 
ATTGAACGAA AGATTAAACT GCAGTATTCC TTGTCACATG AGAAAGATTT AAATAAATAT 
CTAGAGCTCT GCGAAGGCAA GGGCTTAGAT GAGGAAGAGG AGCCTATTGT AGATGCATCT 
CCTCCTTTAA CCAAGGATAC CCAGCGGTCC CGTAGTCCAG CACGTAAAGA AGCTTCAAAG 
TCGCCATATA CTTCACCATC GCGTAAGGCA GTTGCAAAAT CTCCTCATAC TTCTCCTGCA 
CGTAAGCCAG CTACAAAATC TCCTTATACC TCGCCTTCCC GTGCTAGTGA CAAAAAGCGG 
AGCCCTTATA CCTCGCCAGT ACGTCGACAG GCGCATCCCG GTGATTTACC CATCTCCGAT 
GACTTGGAGT ACAAGTATCG TGTGCTTGAT CTGGTACGTT CCAAATCCAA GGAGAACTTG 
TCGAAGCGTA AAACTGATCC CAATAAAAAG CCGCCCATTC ATCCTCTTGA AATGCTACTT 
GATCCGAGTC CCGATGACAG CGAGATACCC ACAACAGGGG AACTGGAGCA CCGCATACGT 
CTGCTGGATG AAAAGCTAAA GTCGCCCTGC CGCCATAAAT CTCGCTCACG TTCACCCACC 
ATCGATGACA TGAAACGAAA GAAGCTCTTG GAGGAAAAGC GACCCAAATC GCCGGTTCAT 
ACATTGGAAC GTTTGGTCAG TTCACCTAAC AGACCAGAGC CACCCACTGA GGAGGAACTA 
GAACAGCGAA TGCGTGCCTT GGAAGACGAA AAGCGTTTCG ACTTCAAGAC CCAAAAGGAT 
TACAAGGCAT TCAATCAAAA GCTTAAAGAT GTCATCTCAC CTTCCCTATC TTTCGAAGAG 
TTCAAGGCGG CCAAATCGCG TGAACAGAGT CCACGGCGTC AAGGAGCCAC AACACCCAAA 
TCAGCAATGC GACGCGACGA TACAGATGAT TACCGAGGAC GCGAGGAGAC ACCCCATTAT 
CGGCCCACTA GTCCTAAGGT GATACGCTTT CGAGATGAGG ATGAGGATTA TTACAAGGAG 
AGCCAGCGTC CGAAGTCTCG ACAGTCCAGC GAACGAATGA AGACTCTTGC AGATCGCAGC 
TACAGCAGCA GCATTGCAGC AGCAACGGCC AGCAGCTCCG AGGGTCTCGA TGCCCTGGGT 
AGTCGCCTAA TGAGAGAAAC CTCGCCAATT ACCCGCACTG GGACTCATAC TGGTGTGCCG 
TTACGGACTG GAGAGAATAT CAATGATCGT CTAAGTTCTA TCAAGGATAC CATCAAATCC 
ATTGACACTC TATGCGAGGA GAAACCGTAT CAGAAGGAGA AATGCCAACG TTATATTGAT 
TCCCTCTTCT CAGATTCATT GCATTTCGGT TGTAAGAAGA ATTCCATGGA GGAGCTAACG 
CACAGTCGCA GCGAGAGTCG AGGACGTACA GCACAACGAT CGAGTGACTA TACGCCGGCC 
ATTCGAGTCA CCTCAGAGCA TCGATCTCTG GGCTCGGCCG ACTCGAAGCG CACAAGTCCA 
TTGCGGGCAT CCAGTCCGTT GCATCATCAT CGCTCGCATA GGGATATCAG TAGGGAGTTA 
TCGCCGCGCA GGCGTCGTGA TGCCGAGGAT ATGGAGGAGC GCGAAAGTAG TAGGGTAAGA 
CGTGATAATA TGTTGCCAAA TTATTTTCTC GATAATCGTA GCGATTTAAG TAGCGGCAGT 
AGTTTAACTA AGTTTCATAA AGTAGATAGA CAACTAGAAG AAACGTGCGC CAAGTACGAA 
GCTGACGATC GACGCTCGGC CAGCAGAACA CCATTGAGTT CGCCATATGA GTCCAGAACA 
ACAGCCACAC GCTACAACAA CAACAACAAC ACAGGCACAG GGACAACAAC AACAACAACC 
GCCGATACAT TTCAAAGGCC CGTATCGCCC TATCGCCAGC CCTATGACCC ATATAGACGT 
AATGTGAATG CCCCATCCCC GGTTCCGGTT TATCAGCCAG CAAAGCTGGA AATTCGTCAT 
ACGACAGTCA CATCAACCTT CTATGATCGT TTTCTCACCG AAAAACAAAT TGAGAAGCAA 
ACACTCTCAC GTCCACCCAG TCGTTCGCCT GTAGTCTCAC CATCGGCCAG CATAGCCAAA 
AGCGATACTG ATCTGCCGGG CACAGTGAGC AGTGCCTCTG CTTCCGCTTC CGCCTCACAA 
TTCGCTTCCT CCTACTCCTC CGCCGCCTAC AAGCCCACAG TAACCACCGC AATCATGTCC 
AGCAGTTATG CTGGTACTGC CACCTCACAT TTTACTTCTT CGTTGCCCAC AAGCAATTAT 
TCATATCTCA TGAGCACTTC CACCAACGCC ACCACCACCA CCACCTCTTC CTCCTCCACT 
ACTGGTGGTT CGTCTGGGGG ATATGTGCCA TATAATTTCA GCAGCTCGTT TAGCAATCGT 
CATAGTGATC CAATTGCCAA TTCTTCTGGC ATCTCGAGCG GTAGCACTGG TGTCTATAAT 
CCCATGATGT CGTTCACTTT GCGTGAACCA ACAAGTGCCT CGGGCAGTCT CTTGGGTCAA 
TATGCCTACA AAAGTAGTTT CGTCTCCAAC TACAGTTCGT CATCCAATCA AATGCCCAAG 
TCACCATCCA CCAGTGATCA GGACAAGCTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6649543 ( XM_023179841.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023035609.1 Drosophila willistoni titin (LOC6649543), transcript variant X4, mRNA.

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CloneID	ODf27216
Clone ID Related Accession (Same CDS sequence)	XM_023179841.1
Accession Version	XM_023179841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	uncharacterized protein LOC6649543 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032872.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTTTA GCCTTTCACC ACCGCCGCAG AAAGCTATTA CCGCCAGCGT AACCTCAACC 
AAGAGTAGCA CAAACATTGA CATTAGCTCC AGCTCCAGCA CCATCACCAC CGCGGCTACA 
ACCAGCACCA CCAAAACAAC AACAACCACT TTCTCCACCA CCACCACCAA CAACAAAAAT 
AATATGCTGG GTCATGAGGG GAACAGCTCA AATTCAGCTG CCTCTATTTC TGCATCAGCT 
GACGAAGATG CCGCCTCGGA TTATAATCAA TGGCTGCATG CCATGAAACT GGTGGCTCGT 
CTACCAGGAG GCACACCACC CGAGTTTCGT CGCAAGCTCT GGCTCTCGTT GGCTGACAAA 
TATCTCAAGT CCAAGAATGT TGATTGGACG CAGCAGCGTG AGAAATGCTT TTGCGAGGAA 
TGGCGTGAAG ATGACGAAGA GCTGGGCATA CAAATTGTCA AGGATTTGCA TCGCACTGGT 
TCTAATTTGT GCACTGGTCC CGCGGGCTCC ATTAACCAGG CCAAGCTGAA GCGCATTCTA 
CTCGGCTATG CCCGCTATAA TCCCGAGGTG GGCTATTGTC AGGGCTTCAA TATGCTGGGT 
GCTTTGATTC TACAAGTCAT GGACAAGGAA GAGGAGGAGT CCATGAAGGT CATGATCTAT 
CTGGTGGAGG GTGTACTGCC AACGGGTTAT TTCTATGGTT CAATGGGCGG TCTTCAGGCG 
GATATGGGAG TCTTTCGCGA GCTTATGCAA ACGAAACTGC CGCGTCTGGC CAAGCATTTG 
CAGCGTTTGC AGGGAGGACC CGTTGAGAAT GCCTACGAAC CGCCATTGAC CAATGTCTTC 
ACCATGCAAT GGTTCCTCAC CATGTTCTGC ACCTGCCTAC CCATGTCCTG TGTCCTGCGC 
GTCTGGGATC TCGTTCTCAT CGAAGGCAGT GATGTTCTAT TGCGCACTGC CCTCGTCCTG 
TGGAGTCTAT TGGAAGAACG TGTCCTTAAT GCTCGCACAG CTGATGAGTT CTATGGCAAA 
ATGGGATCTT TCTCGAGTGA ATTGCTCAAT GGTCATCTTA TCGATTCAAA TGGTCTAATC 
GAAAAGGTTG TTAAACTCGG ACCCATTGCC GATTTGCGTC ATTTGAGGGA TAAACATCTA 
TATAACATAG CCCCATTGAG GCATAAACAA GGCATACAAC TCTATTACGA TGACGATGAT 
ACACATTCGG ATGAGGAGCG CATGGCAGTG GCTACCGTGT GGGGTTTAAA TTGGGGTCGC 
CGCGGTTCGG TGGGACCCGG AGCCCCAGCG GGTAGGCAGC CATCTGAACA AAAGGATCGT 
CTGGCCTTGG ATATATCGTT GCTGAAGAAG CAATACGATC GTCTTCGAGA ACGTCAGAAG 
CAGGCCCATG TCATCCTAAC TACTGCATGC TCAACAGCAA CTCGTCAAAC GCCTTCAGCG 
CAAAGCTCGG TGCCGGTTAA TCAGTTACTA CAGGGTCGAC ACGCCATTGT GACCAACAGG 
GGCAAACGGG TGAGTGCTCC CTTGGGAGCC ATACCTCCAG CCCGAAAGCC TTCACTGCCA 
GCTGTATTGC ACGGAAACGG AAAGCCTATG ATGGGGGAGA AGCAGTTGCG ACGAGGAGAA 
ACCCTACTCT GGCGGGACAC GGACACTAGT CGACGGCGAA GAGACAGCCT CACGTGGAAG 
GAGATCAAAG CAGATCGTGC GGCCATGCTA CGCGAAGGAG TTGATTCAAG CTCTCTAAAG 
ACCCAGAAAC TTCGCACGCG GTTGGGCAAA AGTGACAGTT CGTCTTACAG TGAGGATAGC 
GATGGCGATC AGGATGAGAA AGCAGACAAC GGTGGTGCTG GTGGTGGCTC TAGTACGGAC 
ACCAGTCTCT GTGATGACGA TGATCCCAAG TCAAGCGAAA AAAGCCCCAA GCGTAAGGCG 
AAGGTGGCAC GTAAGCTCAA AGAGAAGAGT CAGCATTTGG GGTCTCGGGA GTCCAGCGCA 
GAGCGTCAAC GACCGAAATC CTGGGCACCA GGCACGCATG AGATACCCTT TATGTTGATG 
GGCATGGACA GCGGTGATGA AAAGGAAGCC AAAAAGGAGG AGCAAGACCT GGAGGAAGAC 
AGTGCCACTG AAAGTGGTCG TTTTAGCTAT CCTAAGGAAC TAGATTGGAC TCAGGCGGCA 
GATAAAGTAG CAAGTTGTGA ACTAGAACCA TTGGATATTG ACAAGATACT GCCTAACACT 
GCTATTTATC TAAGCACAAT CAGTCTAAGT CCGCCCAAGA CGGAAGATGT GGGAGATCAG 
GATGTGATAG AGGAACAACC CATTGTGAAG CAGTTTGACG TTAGTGATTC TGGGGTAACA 
AATCAATATT TTGAGCGTGT CAACAGCGTT GAGCGTCCCA ATAGGTTGGA GCTCTCATAC 
TCTCTAAACG AGGAGGAGGA CTCTACAATG TTAAAAGACG ATCGTCAAAG TTCGGATTTT 
GAATTGAAAA TTGAACCCGA ACCTTCCACT TTAAATAAGG TCCCACAAAT TCGGGATGAT 
AACATACCCG GTGAAAACAA AGATGACTAC AAGGAGCTTT TGAGCATGAC GATAGAAGAG 
CATAAGCCAC CAGCAGCTAC AATCACCAGC CAACTGAATG CGAGTCGAAA GCGTCGGGAT 
CCCAGGCGTA AAACGCTAAC CAGATCCTCC ACAATCGAGA TTGAGGAACG TTTTCAGGCC 
CTCGAACGAA CACTGAGCCA AGATCAACCG ATTCATAAGT ACATACCGAG CACTGCAGCT 
CTCGAAGAAC GCTATAATAG TCTAGAAAAA CAGTTGAGTG CCGAGAAACA GCGGAAGGAG 
GAGGATCTGG ATCGTATACC TTCTACAGCT GATCTAGAAT CACGATTTGA TGCCTTAACG 
AAACAAATGA GTTCCAGCGA ATCGAGCAAT TCCAAGGCCC CACTCGATCT TAATAAAGAT 
CAGCAGCAGC CTGGCGAGAA ATCCAAAGAA ACAGAAAGAA AATCAAACAA AAACCAAAGC 
AAAGAAGGGG AGAAACAAAC CCAATCGGAA CCTAAAGAAA CTGCATCGGA AAAGGAAAGT 
CCTGGCCAAA AAGAAGAAAC CAAAGAAATT GAAGAAGAAC AACAGCCACG TCTCAAGAAA 
CTGCCCTCTA CAGCCGAACT TGAGGATCGA TTTAATGCTT TAGAACGAAA AATGAGCGTA 
CAAAAATCTA GTTCGGCAAA GACAAAGAAA GAGCCACCCG ATGAAGAAAA ACCTGCCCAA 
AAAACTGAAG CTCAAACAAA GGAAACTAAA TCTAAACAAG AATCAAGCCC ACAAAAGACT 
CCAGAAAACA TAGAGACAAG TGAAAAGAGC ACGAAAATCA GTAAGGATTC AAATGAGGTT 
AAGGATAAAG CCGATGAGGT AAAACGAAAA GTTCCACCTT CAACCGAGGA GTTGGAAAAG 
CGTTTCAATG CTTTGGAGAA ACAACTTAGT ACAACTAATT TGGAAGCTGA GCAACCTACT 
ACTTCTAAGG CAAAGGAAAT CAAATCAGAA TTACCCAAGC AAACTGAAAA TGTAAAAGCG 
AACAAGCAAG AAATTCAATC ACAAAAATCC ATCAAGTCTT TCGATGAAAA AGTTAAAGTT 
ATTAATACAA AATTAACAGC CGATGAGCAG AAAATCCAAG ACCACGACAA ATTGGCCGAA 
GAAAAACGTA AAAGTCTTGA TGAGAAAAGT GAAAGAGCCA GTGGTCAGTT AAAAGAAATG 
TTGCCTGAGA ATGAAACCAT TAAATCAAAG ACATCTCAAC CTGAAGTCGT CCGGGCTGAT 
GTTCAACCCA AAGAACTGGG TAAGCGAAGA GCTTCCGAAC CACCATCAAC AGAAGATTTG 
GAGAAACGCT ATGAGACACT TAAGCGTCGC ATGAGTAGTA AAAATCATTT TGGTAATCTA 
ACAGAATCAG CAGATGAGTC CATGTCAAGG CGAGATCAAG AAGTAATATC CGAGACTTTA 
GAGACTGGAG AGCCGGAGAG AAAACAATCA CCGCCGACAA CAGAATATCT GGAAAGTAGG 
TATGAGAAGC TACATGGGGA AAACAAGAAA ACAACGCCCA AGAAAGTGGA TGTGGCGATT 
GAGGCGCATA TATCACCACC ACCAGCTCCG CCACCACCTC CAAAGCCGAA TATCCTAGCT 
GATCCGGCTA TGCATAAGCA ACGAGCTCTC ATTGAAGAGC TACAGGCGAA AATTCAAGAG 
CAAGGACAGT CCACGCCTGG TGAAGAGAAT CTCAAGCCCA GCGAGATAAA TCCACAGCGC 
AAACAGAGGC AAGAGCAACA ACGTCTGCTA CAACGGCCCA CGACAATGGG AGATGAGACC 
TCGGAAGCAC CTGCAAAGAC CGCTTACTAC AGATCGGGAA ATTACGAACC CTGGCAGCAG 
CAGCACCAGC AGAAGCAGCG AATGGTTCGT CGTTTCTCTG ATTTGCCATC AAGAGCCGAT 
CTAGAAAATC GCTTACAATT CCTGGAGAAG CAATTGTACA AGAAATTCTA CAAGCAGCGC 
TGTGCAAGTG ATTCCGAAGT TGCATCGCGG ACGATCGATG ACGAAAAGCC CAGCACATCG 
GCAGCCCGTG TGGCGGAAGT TCAGCTGGAG CAACGTGTCC TGGCCCTAGA GAAGCAATTA 
AGTGAGAATA GTCTAAAGCT GCTAGAGGCC ATGCGTGTCA ACGTGCCCAA CGTCGATGGC 
GGCTCTCCGC GAAGACTAAG CACGGATACG ATAGATGCCA CTGGCAAGGA ACTGGTGCGG 
TATACACAGA ACTTTGGTGA GCTGGACGAG TTGGGTGGAG ATGGCTCAAA TAAGCCCATT 
AACATTAGTA TCAATATCAA AATGATGCTC AACAAGGAAC AGCAACAACA ACGACAACAA 
CAACAGCAGC AGCAGCAGCA GGAGGAAAAG GCAGACACTA GGACCAGCAC AGAGGATTTA 
AGGATGCGCT TGGAGCAATT GGAACAGCAA TTGATGGAAG AAAGAGCCAA GAACAATGGT 
TTTGTGGATG TCGAAAGTGC TAATGATGGC GGAGCAGGAG AGGAGGCAAA GCAATTGGAT 
AAATCCGAAA CTTCAGTACC GGAAGAACTA GAGGAAAATA AAAAGCAAGA GAAGGACAGT 
CACAATCAAA CGGTAAAAAG TGATGAAGCT GAAATGACAG AAGATCCAAT TGTGGCCACC 
ACCGTGGAAA CCATTGAGCC GGAGACAGTG AAAGAAACAA AGACCTTAGA AAACGAGGAG 
ATAGCTCAAG CAACCCAAGA GAAAGCTGCA AAAGCTGAGG AGATCGAAGA GACAACCAAA 
GATAAAATAA ATGAATCATC TGAAGTCATT CCTAAAGTGG AGGAAACTAA ACCGCCAACA 
GAAACAGAAA CCCACTGCGA AGAGAAAACT GTAGACTCTG ACAAGATTAT TGTCAATGCC 
AGCTCTATTG AACCTCCGGT GGATCCCAAT AATAAGACCG TGGTCCTGCT TATGGATAAC 
GAACCTAGAG CTTCGAGAGT GCGCCGTTTG ACCCGCGCCA ATACCGAAGA ACTGGAAGGT 
CTATTCCAGG CTTTAGAAAA ACAACTGCAG GATCGCAATC TAATCAAATC TGAAGATGGG 
CGGTTAATAC GAGCAAGTAC GGAACAGAAA CAAACTGCAG AACAAGTAGA ACAAGCTCAG 
GCTATAAGCG ATCTAACTAA GGAAATCGAA GACTTTACCA GCGGTAAACC AGACGCAAAA 
AGTGAAGGCG AAACAAAGGA AGATAAGCCA GCAGATGGCG GCGAAGAACC CCCCGACGAT 
TATGATTGGG GTCCCAATCC GGTTAAGCAT CATTTGAAAC GCAAAACCGT ATATTTGCCT 
TCAACCAAAG AGCTAGAGGC TCGTTTTCGG TCACTGGAAC GTCAAATTAA ACTACTCGAA 
GATGTGGAGA AAATCGATGT GGAGCAGCGC TTAAATGAAA TTGAACGAAA GATTAAACTG 
CAGTATTCCT TGTCACATGA GAAAGATTTA AATAAATATC TAGAGCTCTG CGAAGGCAAG 
GGCTTAGATG AGGAAGAGGA GCCTATTGTA GATGCATCTC CTCCTTTAAC CAAGGATACC 
CAGCGGTCCC GTAGTCCAGC ACGTAAAGAA GCTTCAAAGT CGCCATATAC TTCACCATCG 
CGTAAGGCAG TTGCAAAATC TCCTCATACT TCTCCTGCAC GTAAGCCAGC TACAAAATCT 
CCTTATACCT CGCCTTCCCG TGCTAGTGAC AAAAAGCGGA GCCCTTATAC CTCGCCAGTA 
CGTCGACAGG CGCATCCCGG TGATTTACCC ATCTCCGATG ACTTGGAGTA CAAGTATCGT 
GTGCTTGATC TGGTACGTTC CAAATCCAAG GAGAACTTGT CGAAGCGTAA AACTGATCCC 
AATAAAAAGC CGCCCATTCA TCCTCTTGAA ATGCTACTTG ATCCGAGTCC CGATGACAGC 
GAGATACCCA CAACAGGGGA ACTGGAGCAC CGCATACGTC TGCTGGATGA AAAGCTAAAG 
TCGCCCTGCC GCCATAAATC TCGCTCACGT TCACCCACCA TCGATGACAT GAAACGAAAG 
AAGCTCTTGG AGGAAAAGCG ACCCAAATCG CCGGTTCATA CATTGGAACG TTTGGTCAGT 
TCACCTAACA GACCAGAGCC ACCCACTGAG GAGGAACTAG AACAGCGAAT GCGTGCCTTG 
GAAGACGAAA AGCGTTTCGA CTTCAAGACC CAAAAGGATT ACAAGGCATT CAATCAAAAG 
CTTAAAGATG TCATCTCACC TTCCCTATCT TTCGAAGAGT TCAAGGCGGC CAAATCGCGT 
GAACAGAGTC CACGGCGTCA AGGAGCCACA ACACCCAAAT CAGCAATGCG ACGCGACGAT 
ACAGATGATT ACCGAGGACG CGAGGAGACA CCCCATTATC GGCCCACTAG TCCTAAGGTG 
ATACGCTTTC GAGATGAGGA TGAGGATTAT TACAAGGAGA GCCAGCGTCC GAAGTCTCGA 
CAGTCCAGCG AACGAATGAA GACTCTTGCA GATCGCAGCT ACAGCAGCAG CATTGCAGCA 
GCAACGGCCA GCAGCTCCGA GGGTCTCGAT GCCCTGGGTA GTCGCCTAAT GAGAGAAACC 
TCGCCAATTA CCCGCACTGG GACTCATACT GGTGTGCCGT TACGGACTGG AGAGAATATC 
AATGATCGTC TAAGTTCTAT CAAGGATACC ATCAAATCCA TTGACACTCT ATGCGAGGAG 
AAACCGTATC AGAAGGAGAA ATGCCAACGT TATATTGATT CCCTCTTCTC AGATTCATTG 
CATTTCGGTT GTAAGAAGAA TTCCATGGAG GAGCTAACGC ACAGTCGCAG CGAGAGTCGA 
GGACGTACAG CACAACGATC GAGTGACTAT ACGCCGGCCA TTCGAGTCAC CTCAGAGCAT 
CGATCTCTGG GCTCGGCCGA CTCGAAGCGC ACAAGTCCAT TGCGGGCATC CAGTCCGTTG 
CATCATCATC GCTCGCATAG GGATATCAGT AGGGAGTTAT CGCCGCGCAG GCGTCGTGAT 
GCCGAGGATA TGGAGGAGCG CGAAAGTAGT AGGGTAAGAC GTGATAATAT GTTGCCAAAT 
TATTTTCTCG ATAATCGTAG CGATTTAAGT AGCGGCAGTA GTTTAACTAA GTTTCATAAA 
GTAGATAGAC AACTAGAAGA AACGTGCGCC AAGTACGAAG CTGACGATCG ACGCTCGGCC 
AGCAGAACAC CATTGAGTTC GCCATATGAG TCCAGAACAA CAGCCACACG CTACAACAAC 
AACAACAACA CAGGCACAGG GACAACAACA ACAACAACCG CCGATACATT TCAAAGGCCC 
GTATCGCCCT ATCGCCAGCC CTATGACCCA TATAGACGTA ATGTGAATGC CCCATCCCCG 
GTTCCGGTTT ATCAGCCAGC AAAGCTGGAA ATTCGTCATA CGACAGTCAC ATCAACCTTC 
TATGATCGTT TTCTCACCGA AAAACAAATT GAGAAGCAAA CACTCTCACG TCCACCCAGT 
CGTTCGCCTG TAGTCTCACC ATCGGCCAGC ATAGCCAAAA GCGATACTGA TCTGCCGGGC 
ACAGTGAGCA GTGCCTCTGC TTCCGCTTCC GCCTCACAAT TCGCTTCCTC CTACTCCTCC 
GCCGCCTACA AGCCCACAGT AACCACCGCA ATCATGTCCA GCAGTTATGC TGGTACTGCC 
ACCTCACATT TTACTTCTTC GTTGCCCACA AGCAATTATT CATATCTCAT GAGCACTTCC 
ACCAACGCCA CCACCACCAC CACCTCTTCC TCCTCCACTA CTGGTGGTTC GTCTGGGGGA 
TATGTGCCAT ATAATTTCAG CAGCTCGTTT AGCAATCGTC ATAGTGATCC AATTGCCAAT 
TCTTCTGGCA TCTCGAGCGG TAGCACTGGT GTCTATAATC CCATGATGTC GTTCACTTTG 
CGTGAACCAA CAAGTGCCTC GGGCAGTCTC TTGGGTCAAT ATGCCTACAA AAGTAGTTTC 
GTCTCCAACT ACAGTTCGTC ATCCAATCAA ATGCCCAAGT CACCATCCAC CAGTGATCAG 
GACAAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023035609.1
CDS	34..8565
Translation	MRFSLSPPPQKAITASVTSTKSSTNIDISSSSSTITTAATTSTTKTTTTTFSTTTTNNKNNMLGHEGNSSNSAASISASADEDAASDYNQWLHAMKLVARLPGGTPPEFRRKLWLSLADKYLKSKNVDWTQQREKCFCEEWREDDEELGIQIVKDLHRTGSNLCTGPAGSINQAKLKRILLGYARYNPEVGYCQGFNMLGALILQVMDKEEEESMKVMIYLVEGVLPTGYFYGSMGGLQADMGVFRELMQTKLPRLAKHLQRLQGGPVENAYEPPLTNVFTMQWFLTMFCTCLPMSCVLRVWDLVLIEGSDVLLRTALVLWSLLEERVLNARTADEFYGKMGSFSSELLNGHLIDSNGLIEKVVKLGPIADLRHLRDKHLYNIAPLRHKQGIQLYYDDDDTHSDEERMAVATVWGLNWGRRGSVGPGAPAGRQPSEQKDRLALDISLLKKQYDRLRERQKQAHVILTTACSTATRQTPSAQSSVPVNQLLQGRHAIVTNRGKRVSAPLGAIPPARKPSLPAVLHGNGKPMMGEKQLRRGETLLWRDTDTSRRRRDSLTWKEIKADRAAMLREGVDSSSLKTQKLRTRLGKSDSSSYSEDSDGDQDEKADNGGAGGGSSTDTSLCDDDDPKSSEKSPKRKAKVARKLKEKSQHLGSRESSAERQRPKSWAPGTHEIPFMLMGMDSGDEKEAKKEEQDLEEDSATESGRFSYPKELDWTQAADKVASCELEPLDIDKILPNTAIYLSTISLSPPKTEDVGDQDVIEEQPIVKQFDVSDSGVTNQYFERVNSVERPNRLELSYSLNEEEDSTMLKDDRQSSDFELKIEPEPSTLNKVPQIRDDNIPGENKDDYKELLSMTIEEHKPPAATITSQLNASRKRRDPRRKTLTRSSTIEIEERFQALERTLSQDQPIHKYIPSTAALEERYNSLEKQLSAEKQRKEEDLDRIPSTADLESRFDALTKQMSSSESSNSKAPLDLNKDQQQPGEKSKETERKSNKNQSKEGEKQTQSEPKETASEKESPGQKEETKEIEEEQQPRLKKLPSTAELEDRFNALERKMSVQKSSSAKTKKEPPDEEKPAQKTEAQTKETKSKQESSPQKTPENIETSEKSTKISKDSNEVKDKADEVKRKVPPSTEELEKRFNALEKQLSTTNLEAEQPTTSKAKEIKSELPKQTENVKANKQEIQSQKSIKSFDEKVKVINTKLTADEQKIQDHDKLAEEKRKSLDEKSERASGQLKEMLPENETIKSKTSQPEVVRADVQPKELGKRRASEPPSTEDLEKRYETLKRRMSSKNHFGNLTESADESMSRRDQEVISETLETGEPERKQSPPTTEYLESRYEKLHGENKKTTPKKVDVAIEAHISPPPAPPPPPKPNILADPAMHKQRALIEELQAKIQEQGQSTPGEENLKPSEINPQRKQRQEQQRLLQRPTTMGDETSEAPAKTAYYRSGNYEPWQQQHQQKQRMVRRFSDLPSRADLENRLQFLEKQLYKKFYKQRCASDSEVASRTIDDEKPSTSAARVAEVQLEQRVLALEKQLSENSLKLLEAMRVNVPNVDGGSPRRLSTDTIDATGKELVRYTQNFGELDELGGDGSNKPINISINIKMMLNKEQQQQRQQQQQQQQQEEKADTRTSTEDLRMRLEQLEQQLMEERAKNNGFVDVESANDGGAGEEAKQLDKSETSVPEELEENKKQEKDSHNQTVKSDEAEMTEDPIVATTVETIEPETVKETKTLENEEIAQATQEKAAKAEEIEETTKDKINESSEVIPKVEETKPPTETETHCEEKTVDSDKIIVNASSIEPPVDPNNKTVVLLMDNEPRASRVRRLTRANTEELEGLFQALEKQLQDRNLIKSEDGRLIRASTEQKQTAEQVEQAQAISDLTKEIEDFTSGKPDAKSEGETKEDKPADGGEEPPDDYDWGPNPVKHHLKRKTVYLPSTKELEARFRSLERQIKLLEDVEKIDVEQRLNEIERKIKLQYSLSHEKDLNKYLELCEGKGLDEEEEPIVDASPPLTKDTQRSRSPARKEASKSPYTSPSRKAVAKSPHTSPARKPATKSPYTSPSRASDKKRSPYTSPVRRQAHPGDLPISDDLEYKYRVLDLVRSKSKENLSKRKTDPNKKPPIHPLEMLLDPSPDDSEIPTTGELEHRIRLLDEKLKSPCRHKSRSRSPTIDDMKRKKLLEEKRPKSPVHTLERLVSSPNRPEPPTEEELEQRMRALEDEKRFDFKTQKDYKAFNQKLKDVISPSLSFEEFKAAKSREQSPRRQGATTPKSAMRRDDTDDYRGREETPHYRPTSPKVIRFRDEDEDYYKESQRPKSRQSSERMKTLADRSYSSSIAAATASSSEGLDALGSRLMRETSPITRTGTHTGVPLRTGENINDRLSSIKDTIKSIDTLCEEKPYQKEKCQRYIDSLFSDSLHFGCKKNSMEELTHSRSESRGRTAQRSSDYTPAIRVTSEHRSLGSADSKRTSPLRASSPLHHHRSHRDISRELSPRRRRDAEDMEERESSRVRRDNMLPNYFLDNRSDLSSGSSLTKFHKVDRQLEETCAKYEADDRRSASRTPLSSPYESRTTATRYNNNNNTGTGTTTTTTADTFQRPVSPYRQPYDPYRRNVNAPSPVPVYQPAKLEIRHTTVTSTFYDRFLTEKQIEKQTLSRPPSRSPVVSPSASIAKSDTDLPGTVSSASASASASQFASSYSSAAYKPTVTTAIMSSSYAGTATSHFTSSLPTSNYSYLMSTSTNATTTTTSSSSTTGGSSGGYVPYNFSSSFSNRHSDPIANSSGISSGSTGVYNPMMSFTLREPTSASGSLLGQYAYKSSFVSNYSSSSNQMPKSPSTSDQDKL

Target ORF information:

RefSeq Version	XM_023179841.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni titin (LOC6649543), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023179841.1

ORF Insert Sequence:

ATGCGTTTTA GCCTTTCACC ACCGCCGCAG AAAGCTATTA CCGCCAGCGT AACCTCAACC 
AAGAGTAGCA CAAACATTGA CATTAGCTCC AGCTCCAGCA CCATCACCAC CGCGGCTACA 
ACCAGCACCA CCAAAACAAC AACAACCACT TTCTCCACCA CCACCACCAA CAACAAAAAT 
AATATGCTGG GTCATGAGGG GAACAGCTCA AATTCAGCTG CCTCTATTTC TGCATCAGCT 
GACGAAGATG CCGCCTCGGA TTATAATCAA TGGCTGCATG CCATGAAACT GGTGGCTCGT 
CTACCAGGAG GCACACCACC CGAGTTTCGT CGCAAGCTCT GGCTCTCGTT GGCTGACAAA 
TATCTCAAGT CCAAGAATGT TGATTGGACG CAGCAGCGTG AGAAATGCTT TTGCGAGGAA 
TGGCGTGAAG ATGACGAAGA GCTGGGCATA CAAATTGTCA AGGATTTGCA TCGCACTGGT 
TCTAATTTGT GCACTGGTCC CGCGGGCTCC ATTAACCAGG CCAAGCTGAA GCGCATTCTA 
CTCGGCTATG CCCGCTATAA TCCCGAGGTG GGCTATTGTC AGGGCTTCAA TATGCTGGGT 
GCTTTGATTC TACAAGTCAT GGACAAGGAA GAGGAGGAGT CCATGAAGGT CATGATCTAT 
CTGGTGGAGG GTGTACTGCC AACGGGTTAT TTCTATGGTT CAATGGGCGG TCTTCAGGCG 
GATATGGGAG TCTTTCGCGA GCTTATGCAA ACGAAACTGC CGCGTCTGGC CAAGCATTTG 
CAGCGTTTGC AGGGAGGACC CGTTGAGAAT GCCTACGAAC CGCCATTGAC CAATGTCTTC 
ACCATGCAAT GGTTCCTCAC CATGTTCTGC ACCTGCCTAC CCATGTCCTG TGTCCTGCGC 
GTCTGGGATC TCGTTCTCAT CGAAGGCAGT GATGTTCTAT TGCGCACTGC CCTCGTCCTG 
TGGAGTCTAT TGGAAGAACG TGTCCTTAAT GCTCGCACAG CTGATGAGTT CTATGGCAAA 
ATGGGATCTT TCTCGAGTGA ATTGCTCAAT GGTCATCTTA TCGATTCAAA TGGTCTAATC 
GAAAAGGTTG TTAAACTCGG ACCCATTGCC GATTTGCGTC ATTTGAGGGA TAAACATCTA 
TATAACATAG CCCCATTGAG GCATAAACAA GGCATACAAC TCTATTACGA TGACGATGAT 
ACACATTCGG ATGAGGAGCG CATGGCAGTG GCTACCGTGT GGGGTTTAAA TTGGGGTCGC 
CGCGGTTCGG TGGGACCCGG AGCCCCAGCG GGTAGGCAGC CATCTGAACA AAAGGATCGT 
CTGGCCTTGG ATATATCGTT GCTGAAGAAG CAATACGATC GTCTTCGAGA ACGTCAGAAG 
CAGGCCCATG TCATCCTAAC TACTGCATGC TCAACAGCAA CTCGTCAAAC GCCTTCAGCG 
CAAAGCTCGG TGCCGGTTAA TCAGTTACTA CAGGGTCGAC ACGCCATTGT GACCAACAGG 
GGCAAACGGG TGAGTGCTCC CTTGGGAGCC ATACCTCCAG CCCGAAAGCC TTCACTGCCA 
GCTGTATTGC ACGGAAACGG AAAGCCTATG ATGGGGGAGA AGCAGTTGCG ACGAGGAGAA 
ACCCTACTCT GGCGGGACAC GGACACTAGT CGACGGCGAA GAGACAGCCT CACGTGGAAG 
GAGATCAAAG CAGATCGTGC GGCCATGCTA CGCGAAGGAG TTGATTCAAG CTCTCTAAAG 
ACCCAGAAAC TTCGCACGCG GTTGGGCAAA AGTGACAGTT CGTCTTACAG TGAGGATAGC 
GATGGCGATC AGGATGAGAA AGCAGACAAC GGTGGTGCTG GTGGTGGCTC TAGTACGGAC 
ACCAGTCTCT GTGATGACGA TGATCCCAAG TCAAGCGAAA AAAGCCCCAA GCGTAAGGCG 
AAGGTGGCAC GTAAGCTCAA AGAGAAGAGT CAGCATTTGG GGTCTCGGGA GTCCAGCGCA 
GAGCGTCAAC GACCGAAATC CTGGGCACCA GGCACGCATG AGATACCCTT TATGTTGATG 
GGCATGGACA GCGGTGATGA AAAGGAAGCC AAAAAGGAGG AGCAAGACCT GGAGGAAGAC 
AGTGCCACTG AAAGTGGTCG TTTTAGCTAT CCTAAGGAAC TAGATTGGAC TCAGGCGGCA 
GATAAAGTAG CAAGTTGTGA ACTAGAACCA TTGGATATTG ACAAGATACT GCCTAACACT 
GCTATTTATC TAAGCACAAT CAGTCTAAGT CCGCCCAAGA CGGAAGATGT GGGAGATCAG 
GATGTGATAG AGGAACAACC CATTGTGAAG CAGTTTGACG TTAGTGATTC TGGGGTAACA 
AATCAATATT TTGAGCGTGT CAACAGCGTT GAGCGTCCCA ATAGGTTGGA GCTCTCATAC 
TCTCTAAACG AGGAGGAGGA CTCTACAATG TTAAAAGACG ATCGTCAAAG TTCGGATTTT 
GAATTGAAAA TTGAACCCGA ACCTTCCACT TTAAATAAGG TCCCACAAAT TCGGGATGAT 
AACATACCCG GTGAAAACAA AGATGACTAC AAGGAGCTTT TGAGCATGAC GATAGAAGAG 
CATAAGCCAC CAGCAGCTAC AATCACCAGC CAACTGAATG CGAGTCGAAA GCGTCGGGAT 
CCCAGGCGTA AAACGCTAAC CAGATCCTCC ACAATCGAGA TTGAGGAACG TTTTCAGGCC 
CTCGAACGAA CACTGAGCCA AGATCAACCG ATTCATAAGT ACATACCGAG CACTGCAGCT 
CTCGAAGAAC GCTATAATAG TCTAGAAAAA CAGTTGAGTG CCGAGAAACA GCGGAAGGAG 
GAGGATCTGG ATCGTATACC TTCTACAGCT GATCTAGAAT CACGATTTGA TGCCTTAACG 
AAACAAATGA GTTCCAGCGA ATCGAGCAAT TCCAAGGCCC CACTCGATCT TAATAAAGAT 
CAGCAGCAGC CTGGCGAGAA ATCCAAAGAA ACAGAAAGAA AATCAAACAA AAACCAAAGC 
AAAGAAGGGG AGAAACAAAC CCAATCGGAA CCTAAAGAAA CTGCATCGGA AAAGGAAAGT 
CCTGGCCAAA AAGAAGAAAC CAAAGAAATT GAAGAAGAAC AACAGCCACG TCTCAAGAAA 
CTGCCCTCTA CAGCCGAACT TGAGGATCGA TTTAATGCTT TAGAACGAAA AATGAGCGTA 
CAAAAATCTA GTTCGGCAAA GACAAAGAAA GAGCCACCCG ATGAAGAAAA ACCTGCCCAA 
AAAACTGAAG CTCAAACAAA GGAAACTAAA TCTAAACAAG AATCAAGCCC ACAAAAGACT 
CCAGAAAACA TAGAGACAAG TGAAAAGAGC ACGAAAATCA GTAAGGATTC AAATGAGGTT 
AAGGATAAAG CCGATGAGGT AAAACGAAAA GTTCCACCTT CAACCGAGGA GTTGGAAAAG 
CGTTTCAATG CTTTGGAGAA ACAACTTAGT ACAACTAATT TGGAAGCTGA GCAACCTACT 
ACTTCTAAGG CAAAGGAAAT CAAATCAGAA TTACCCAAGC AAACTGAAAA TGTAAAAGCG 
AACAAGCAAG AAATTCAATC ACAAAAATCC ATCAAGTCTT TCGATGAAAA AGTTAAAGTT 
ATTAATACAA AATTAACAGC CGATGAGCAG AAAATCCAAG ACCACGACAA ATTGGCCGAA 
GAAAAACGTA AAAGTCTTGA TGAGAAAAGT GAAAGAGCCA GTGGTCAGTT AAAAGAAATG 
TTGCCTGAGA ATGAAACCAT TAAATCAAAG ACATCTCAAC CTGAAGTCGT CCGGGCTGAT 
GTTCAACCCA AAGAACTGGG TAAGCGAAGA GCTTCCGAAC CACCATCAAC AGAAGATTTG 
GAGAAACGCT ATGAGACACT TAAGCGTCGC ATGAGTAGTA AAAATCATTT TGGTAATCTA 
ACAGAATCAG CAGATGAGTC CATGTCAAGG CGAGATCAAG AAGTAATATC CGAGACTTTA 
GAGACTGGAG AGCCGGAGAG AAAACAATCA CCGCCGACAA CAGAATATCT GGAAAGTAGG 
TATGAGAAGC TACATGGGGA AAACAAGAAA ACAACGCCCA AGAAAGTGGA TGTGGCGATT 
GAGGCGCATA TATCACCACC ACCAGCTCCG CCACCACCTC CAAAGCCGAA TATCCTAGCT 
GATCCGGCTA TGCATAAGCA ACGAGCTCTC ATTGAAGAGC TACAGGCGAA AATTCAAGAG 
CAAGGACAGT CCACGCCTGG TGAAGAGAAT CTCAAGCCCA GCGAGATAAA TCCACAGCGC 
AAACAGAGGC AAGAGCAACA ACGTCTGCTA CAACGGCCCA CGACAATGGG AGATGAGACC 
TCGGAAGCAC CTGCAAAGAC CGCTTACTAC AGATCGGGAA ATTACGAACC CTGGCAGCAG 
CAGCACCAGC AGAAGCAGCG AATGGTTCGT CGTTTCTCTG ATTTGCCATC AAGAGCCGAT 
CTAGAAAATC GCTTACAATT CCTGGAGAAG CAATTGTACA AGAAATTCTA CAAGCAGCGC 
TGTGCAAGTG ATTCCGAAGT TGCATCGCGG ACGATCGATG ACGAAAAGCC CAGCACATCG 
GCAGCCCGTG TGGCGGAAGT TCAGCTGGAG CAACGTGTCC TGGCCCTAGA GAAGCAATTA 
AGTGAGAATA GTCTAAAGCT GCTAGAGGCC ATGCGTGTCA ACGTGCCCAA CGTCGATGGC 
GGCTCTCCGC GAAGACTAAG CACGGATACG ATAGATGCCA CTGGCAAGGA ACTGGTGCGG 
TATACACAGA ACTTTGGTGA GCTGGACGAG TTGGGTGGAG ATGGCTCAAA TAAGCCCATT 
AACATTAGTA TCAATATCAA AATGATGCTC AACAAGGAAC AGCAACAACA ACGACAACAA 
CAACAGCAGC AGCAGCAGCA GGAGGAAAAG GCAGACACTA GGACCAGCAC AGAGGATTTA 
AGGATGCGCT TGGAGCAATT GGAACAGCAA TTGATGGAAG AAAGAGCCAA GAACAATGGT 
TTTGTGGATG TCGAAAGTGC TAATGATGGC GGAGCAGGAG AGGAGGCAAA GCAATTGGAT 
AAATCCGAAA CTTCAGTACC GGAAGAACTA GAGGAAAATA AAAAGCAAGA GAAGGACAGT 
CACAATCAAA CGGTAAAAAG TGATGAAGCT GAAATGACAG AAGATCCAAT TGTGGCCACC 
ACCGTGGAAA CCATTGAGCC GGAGACAGTG AAAGAAACAA AGACCTTAGA AAACGAGGAG 
ATAGCTCAAG CAACCCAAGA GAAAGCTGCA AAAGCTGAGG AGATCGAAGA GACAACCAAA 
GATAAAATAA ATGAATCATC TGAAGTCATT CCTAAAGTGG AGGAAACTAA ACCGCCAACA 
GAAACAGAAA CCCACTGCGA AGAGAAAACT GTAGACTCTG ACAAGATTAT TGTCAATGCC 
AGCTCTATTG AACCTCCGGT GGATCCCAAT AATAAGACCG TGGTCCTGCT TATGGATAAC 
GAACCTAGAG CTTCGAGAGT GCGCCGTTTG ACCCGCGCCA ATACCGAAGA ACTGGAAGGT 
CTATTCCAGG CTTTAGAAAA ACAACTGCAG GATCGCAATC TAATCAAATC TGAAGATGGG 
CGGTTAATAC GAGCAAGTAC GGAACAGAAA CAAACTGCAG AACAAGTAGA ACAAGCTCAG 
GCTATAAGCG ATCTAACTAA GGAAATCGAA GACTTTACCA GCGGTAAACC AGACGCAAAA 
AGTGAAGGCG AAACAAAGGA AGATAAGCCA GCAGATGGCG GCGAAGAACC CCCCGACGAT 
TATGATTGGG GTCCCAATCC GGTTAAGCAT CATTTGAAAC GCAAAACCGT ATATTTGCCT 
TCAACCAAAG AGCTAGAGGC TCGTTTTCGG TCACTGGAAC GTCAAATTAA ACTACTCGAA 
GATGTGGAGA AAATCGATGT GGAGCAGCGC TTAAATGAAA TTGAACGAAA GATTAAACTG 
CAGTATTCCT TGTCACATGA GAAAGATTTA AATAAATATC TAGAGCTCTG CGAAGGCAAG 
GGCTTAGATG AGGAAGAGGA GCCTATTGTA GATGCATCTC CTCCTTTAAC CAAGGATACC 
CAGCGGTCCC GTAGTCCAGC ACGTAAAGAA GCTTCAAAGT CGCCATATAC TTCACCATCG 
CGTAAGGCAG TTGCAAAATC TCCTCATACT TCTCCTGCAC GTAAGCCAGC TACAAAATCT 
CCTTATACCT CGCCTTCCCG TGCTAGTGAC AAAAAGCGGA GCCCTTATAC CTCGCCAGTA 
CGTCGACAGG CGCATCCCGG TGATTTACCC ATCTCCGATG ACTTGGAGTA CAAGTATCGT 
GTGCTTGATC TGGTACGTTC CAAATCCAAG GAGAACTTGT CGAAGCGTAA AACTGATCCC 
AATAAAAAGC CGCCCATTCA TCCTCTTGAA ATGCTACTTG ATCCGAGTCC CGATGACAGC 
GAGATACCCA CAACAGGGGA ACTGGAGCAC CGCATACGTC TGCTGGATGA AAAGCTAAAG 
TCGCCCTGCC GCCATAAATC TCGCTCACGT TCACCCACCA TCGATGACAT GAAACGAAAG 
AAGCTCTTGG AGGAAAAGCG ACCCAAATCG CCGGTTCATA CATTGGAACG TTTGGTCAGT 
TCACCTAACA GACCAGAGCC ACCCACTGAG GAGGAACTAG AACAGCGAAT GCGTGCCTTG 
GAAGACGAAA AGCGTTTCGA CTTCAAGACC CAAAAGGATT ACAAGGCATT CAATCAAAAG 
CTTAAAGATG TCATCTCACC TTCCCTATCT TTCGAAGAGT TCAAGGCGGC CAAATCGCGT 
GAACAGAGTC CACGGCGTCA AGGAGCCACA ACACCCAAAT CAGCAATGCG ACGCGACGAT 
ACAGATGATT ACCGAGGACG CGAGGAGACA CCCCATTATC GGCCCACTAG TCCTAAGGTG 
ATACGCTTTC GAGATGAGGA TGAGGATTAT TACAAGGAGA GCCAGCGTCC GAAGTCTCGA 
CAGTCCAGCG AACGAATGAA GACTCTTGCA GATCGCAGCT ACAGCAGCAG CATTGCAGCA 
GCAACGGCCA GCAGCTCCGA GGGTCTCGAT GCCCTGGGTA GTCGCCTAAT GAGAGAAACC 
TCGCCAATTA CCCGCACTGG GACTCATACT GGTGTGCCGT TACGGACTGG AGAGAATATC 
AATGATCGTC TAAGTTCTAT CAAGGATACC ATCAAATCCA TTGACACTCT ATGCGAGGAG 
AAACCGTATC AGAAGGAGAA ATGCCAACGT TATATTGATT CCCTCTTCTC AGATTCATTG 
CATTTCGGTT GTAAGAAGAA TTCCATGGAG GAGCTAACGC ACAGTCGCAG CGAGAGTCGA 
GGACGTACAG CACAACGATC GAGTGACTAT ACGCCGGCCA TTCGAGTCAC CTCAGAGCAT 
CGATCTCTGG GCTCGGCCGA CTCGAAGCGC ACAAGTCCAT TGCGGGCATC CAGTCCGTTG 
CATCATCATC GCTCGCATAG GGATATCAGT AGGGAGTTAT CGCCGCGCAG GCGTCGTGAT 
GCCGAGGATA TGGAGGAGCG CGAAAGTAGT AGGGTAAGAC GTGATAATAT GTTGCCAAAT 
TATTTTCTCG ATAATCGTAG CGATTTAAGT AGCGGCAGTA GTTTAACTAA GTTTCATAAA 
GTAGATAGAC AACTAGAAGA AACGTGCGCC AAGTACGAAG CTGACGATCG ACGCTCGGCC 
AGCAGAACAC CATTGAGTTC GCCATATGAG TCCAGAACAA CAGCCACACG CTACAACAAC 
AACAACAACA CAGGCACAGG GACAACAACA ACAACAACCG CCGATACATT TCAAAGGCCC 
GTATCGCCCT ATCGCCAGCC CTATGACCCA TATAGACGTA ATGTGAATGC CCCATCCCCG 
GTTCCGGTTT ATCAGCCAGC AAAGCTGGAA ATTCGTCATA CGACAGTCAC ATCAACCTTC 
TATGATCGTT TTCTCACCGA AAAACAAATT GAGAAGCAAA CACTCTCACG TCCACCCAGT 
CGTTCGCCTG TAGTCTCACC ATCGGCCAGC ATAGCCAAAA GCGATACTGA TCTGCCGGGC 
ACAGTGAGCA GTGCCTCTGC TTCCGCTTCC GCCTCACAAT TCGCTTCCTC CTACTCCTCC 
GCCGCCTACA AGCCCACAGT AACCACCGCA ATCATGTCCA GCAGTTATGC TGGTACTGCC 
ACCTCACATT TTACTTCTTC GTTGCCCACA AGCAATTATT CATATCTCAT GAGCACTTCC 
ACCAACGCCA CCACCACCAC CACCTCTTCC TCCTCCACTA CTGGTGGTTC GTCTGGGGGA 
TATGTGCCAT ATAATTTCAG CAGCTCGTTT AGCAATCGTC ATAGTGATCC AATTGCCAAT 
TCTTCTGGCA TCTCGAGCGG TAGCACTGGT GTCTATAATC CCATGATGTC GTTCACTTTG 
CGTGAACCAA CAAGTGCCTC GGGCAGTCTC TTGGGTCAAT ATGCCTACAA AAGTAGTTTC 
GTCTCCAACT ACAGTTCGTC ATCCAATCAA ATGCCCAAGT CACCATCCAC CAGTGATCAG 
GACAAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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LOC6649543 cDNA ORF clone, Drosophila willistoni

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