DWIL\GK25865 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DWIL\GK25865
Entrez Gene ID	6649022
Full Name	DNA topoisomerase 1
Synonyms	GK25865,GK25865-PA,GK25865-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_002071529.3	XP_002071565.1	DNA topoisomerase 1
XM_015177230.1	XP_015032716.1	uncharacterized protein, isoform B
XM_002071529.2	XP_002071565.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DWIL\GK25865 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWIL\GK25865 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODf11754	XM_002071529.3 Latest version!	Drosophila willistoni DNA topoisomerase 1 (LOC6649022), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODf17229	XM_015177230.1 Latest version!	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK25865), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODf11754	XM_002071529.2	Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK25865), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWIL\GK25865 ( XM_002071529.3 ) cDNA ORF clone, Drosophila willistoni -> XP_002071565.1 Drosophila willistoni DNA topoisomerase 1 (LOC6649022), mRNA.

Price & Availability↑

CloneID	ODf11754
Clone ID Related Accession (Same CDS sequence)	XM_002071529.3 , XM_002071529.2
Accession Version	XM_002071529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	DNA topoisomerase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 4, 2017 this sequence version replaced XM_002071529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGGG ATGTGGCTGC TGAAAATAAC ATCCACATAC AAAATGGTGG AAGCTGCAGC 
GAAGTGCAAT CAAATGGTGT ATCGAATGGC CATCATAACC ACTACCATCA TAATAGCAGT 
AGCAGTAGCA GCAAACATAA ATCGTCTAGC AAGGATAAGC ATCGTGATAA GGAGAGAAGT 
GAGCATAAGA GTTCCAGTTC ATCCAAGGAG CATAAGAGTA GCAGTCGCGA CAAGGACAGG 
CATAAGAGCA GCAGCAGTGG CGGTAGCTCC TCGTCCAAGC ATAGGGATAA GGATCATCAT 
CGCAGTAGCT CCTCATCGTC AAGTCATCGT GATAAGGATG GAAGCAGCAG TAGCAGCAGC 
AAACACAAGT CCTCATCATC ATCATCGTCT GGCCACCATA AGAGCTCATC GAAGGATAAA 
GAACGCTCTA AGGACAAAGA TCGTAGCAAC AGTAGTAGCA GCAGCAGCAG CAGTAGCCGA 
GATAAGCGCC GCAGCGATCA CAAAAGCTCA TCTTCATCGA GCTCACGGCA CAGCAGTAGC 
TCCTCGCCAT CGAAAAGCAA ACATTCCAGT TCACGTCACA GTTCATCATC TGGCACTACT 
AGCAATTCTG GCAAGGATCC CTCCTATGAT GGTGTCTTTG TGAAACCGGA GCCAGTTTCT 
CAGCCCCCGA TGCAACAGCA TTATGCTGAA TGCTCAGCAG CCCCATCTGT AGCCAGCAAT 
GGTAATGGTG ATGATGGCAT GATTTACAAA AATGGCTACG AGGAGAACAT TGTCGATATC 
AAGAAGGAGG AGGACAACAG TTTCAATAAT CTATCACAGG CCAGTTCCTG TGACTACTCC 
TTGTCACAAT TTCGATCGGA TGAGCCATCG TTCGAGACAA AGCATGAGCA GCCATCCTAT 
GCTGAAGAGG ATAGTGCCCT CCAGGATGAC GATTACAATA ACGACAATGA CGATGACAAC 
GACGACAACA ACGAGAATGG AGCCGATGAG GATGATGAAG AAGATGACGA GGAGGATGTA 
CCATTGGCCA TGCGCAAACG AAAACAGGAA CCAATTGCTA ATAATGGGGA TGGCTATATG 
GATGATTATA ATGATGACAT TCCGCTGTTG GCGCGTAAGA AGATCAAAAA GGAGCCCAAA 
GAGAAGAGTC GTAAGCAAAA GAAGCGTCCC AAAGAAGAAC TTGATGATGA TTATGGCAGC 
ATGAAGCCCA AAATGAAAAA GGTTAAAAAG GAACCTGAGA TTAAGAGTGA AACTGTATCG 
CCCACCAAAC GTGCCCGTAA AGACAAAAAG GATGAGGAAG AAGAAGTCTG GAGATGGTGG 
GAAGAGGAGA AGCGTGAAGA TGGCGTCAAA TGGTCAACCC TAGAGCATAA GGGTCCTGTC 
TTTGCTCCAC CCTATGAGCG TGTGCCACGC AATGTACGCT TCTTTTACGA TGGCAAACCC 
TTGGATCTTA CCGAAGAAAC CGAAGAGGCA GCCACATTCT ATGCCAAAAT GTTAAATCAT 
GATTACTGCA CCAAGGATGT TTTCAATACC AATTTCTTCA AAGATTTCCG CAAAACCATG 
ACGGACAGGG AGAAAGAAAT TATCAAAGAT TTTCGTAAAT GCAATTTCCA AGAGATGTTT 
GAATTCTTTC AAGCCGAATC GGAGAGACGA AAGGCCGCCA CAAAGGATGA GAAACTGGCG 
AAAAAGAATG AGAACGAGGC ATTGGTTAAG GAATATGGCT TCTGTATGAT CGATGGTCAT 
AAAGAGAAAA TTGGTAATTT CCGTCTAGAG CCGCCGGGTC TATTTCGTGG TCGTGGTGAA 
CATCCGAAAA TGGGAATGAT CAAACGCCGC ATTGGGGCCT CTGAGGTATC GATTAATTGT 
GGTAAAAATT CCAAGGTACC AACACCACCA CCGGGCCAAC GTTGGAAGGA AGTACGTCAC 
GATAATACCG TCACTTGGCT AGCATCTTGG GTAGAGAATG TCCAGGGTCA AGTTAAATAT 
ATTATGTTGA ATCCATCGTC CAAGCTAAAG GGTGAAAAGG ATCACATTAA ATATGAGACA 
GCTCGCCGTT TGGATAAAGT GATTGATAAG ATTCGTGCCA CCTATCGTGA TGAATGGAAA 
TCTAAGGAGA TGCGTGTGCG TCAAAGAGCT GTTGCTTTGT ACTTTATCGA TAAATTAGCC 
CTGAGAGCGG GTAACGAAAA GGATGAGGAT CAGGCGGATA CTGTCGGCTG TTGTTCGTTG 
CGTGTCGAGC ATGTCCAACT GCATAGAGAG CTCAATGGCA AAGAGAATGT GGTTGTGTTC 
GATTTCCCTG GTAAGGATTC CATACGATAC TACAATGAGG TCGAGGTGGA GAAGCGTGTC 
TTTAAAAATC TCGAACTCTT TATGGAGAAT AAGAAGGAGG GCGATGATCT CTTCGATCGT 
CTCAATACCC AAGTTCTCAA CGAGCATCTT AAAGAGTTGA TGGATGGACT TACGGCTAAA 
GTATTTCGTA CATATAACGC TTCAAAAACG CTACAAGATC AATTGGATAA ACTAACCGAT 
GGCGATGCAA CAGTCGCTGA AAAATTGTTG GCCTATAATC GTGCCAATCG TGCTGTGGCC 
ATCCTTTGTA ACCATCAGCG TTCGGTGCCC AAGGGTCACC AAAAGTCCAT GGAGAATTTA 
AAAGAAAAAA TACGCGCCAA GCGCGATGCC ATTGATGATT GTGAATCTGA ATATCATGAT 
TTGAAAAAGG CGGCCAAACG TGGCTCGGTT AAAGAGAAAG TGAATGCCGA TAAGAAGGGC 
AAGCAGCTGG AACGTTTAAA GGAACAGCTA AAGAAGTTGG AACTACAAGA GACTGATAGA 
GACGAGAATA AGACCATTGC CTTGAGCACA TCCAAATTGA ATTATCTCGA TCCACGTATT 
TCGGTGGCCT GGTGTAAAAA GAATGGCGTG CCGATTGAAA AGATCTTTAA TAAAACGCAA 
AGAACCAAAT TTCTTTGGGC TATACATATG GCCGATGAGA ATTATCGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002071565.1
CDS	355..3348
Translation	MSGDVAAENNIHIQNGGSCSEVQSNGVSNGHHNHYHHNSSSSSSKHKSSSKDKHRDKERSEHKSSSSSKEHKSSSRDKDRHKSSSSGGSSSSKHRDKDHHRSSSSSSSHRDKDGSSSSSSKHKSSSSSSSGHHKSSSKDKERSKDKDRSNSSSSSSSSSRDKRRSDHKSSSSSSSRHSSSSSPSKSKHSSSRHSSSSGTTSNSGKDPSYDGVFVKPEPVSQPPMQQHYAECSAAPSVASNGNGDDGMIYKNGYEENIVDIKKEEDNSFNNLSQASSCDYSLSQFRSDEPSFETKHEQPSYAEEDSALQDDDYNNDNDDDNDDNNENGADEDDEEDDEEDVPLAMRKRKQEPIANNGDGYMDDYNDDIPLLARKKIKKEPKEKSRKQKKRPKEELDDDYGSMKPKMKKVKKEPEIKSETVSPTKRARKDKKDEEEEVWRWWEEEKREDGVKWSTLEHKGPVFAPPYERVPRNVRFFYDGKPLDLTEETEEAATFYAKMLNHDYCTKDVFNTNFFKDFRKTMTDREKEIIKDFRKCNFQEMFEFFQAESERRKAATKDEKLAKKNENEALVKEYGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIGASEVSINCGKNSKVPTPPPGQRWKEVRHDNTVTWLASWVENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHRELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMENKKEGDDLFDRLNTQVLNEHLKELMDGLTAKVFRTYNASKTLQDQLDKLTDGDATVAEKLLAYNRANRAVAILCNHQRSVPKGHQKSMENLKEKIRAKRDAIDDCESEYHDLKKAAKRGSVKEKVNADKKGKQLERLKEQLKKLELQETDRDENKTIALSTSKLNYLDPRISVAWCKKNGVPIEKIFNKTQRTKFLWAIHMADENYRF

Target ORF information:

RefSeq Version	XM_002071529.3
Organism	Drosophila willistoni
Definition	Drosophila willistoni DNA topoisomerase 1 (LOC6649022), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002071529.3

ORF Insert Sequence:

ATGAGTGGGG ATGTGGCTGC TGAAAATAAC ATCCACATAC AAAATGGTGG AAGCTGCAGC 
GAAGTGCAAT CAAATGGTGT ATCGAATGGC CATCATAACC ACTACCATCA TAATAGCAGT 
AGCAGTAGCA GCAAACATAA ATCGTCTAGC AAGGATAAGC ATCGTGATAA GGAGAGAAGT 
GAGCATAAGA GTTCCAGTTC ATCCAAGGAG CATAAGAGTA GCAGTCGCGA CAAGGACAGG 
CATAAGAGCA GCAGCAGTGG CGGTAGCTCC TCGTCCAAGC ATAGGGATAA GGATCATCAT 
CGCAGTAGCT CCTCATCGTC AAGTCATCGT GATAAGGATG GAAGCAGCAG TAGCAGCAGC 
AAACACAAGT CCTCATCATC ATCATCGTCT GGCCACCATA AGAGCTCATC GAAGGATAAA 
GAACGCTCTA AGGACAAAGA TCGTAGCAAC AGTAGTAGCA GCAGCAGCAG CAGTAGCCGA 
GATAAGCGCC GCAGCGATCA CAAAAGCTCA TCTTCATCGA GCTCACGGCA CAGCAGTAGC 
TCCTCGCCAT CGAAAAGCAA ACATTCCAGT TCACGTCACA GTTCATCATC TGGCACTACT 
AGCAATTCTG GCAAGGATCC CTCCTATGAT GGTGTCTTTG TGAAACCGGA GCCAGTTTCT 
CAGCCCCCGA TGCAACAGCA TTATGCTGAA TGCTCAGCAG CCCCATCTGT AGCCAGCAAT 
GGTAATGGTG ATGATGGCAT GATTTACAAA AATGGCTACG AGGAGAACAT TGTCGATATC 
AAGAAGGAGG AGGACAACAG TTTCAATAAT CTATCACAGG CCAGTTCCTG TGACTACTCC 
TTGTCACAAT TTCGATCGGA TGAGCCATCG TTCGAGACAA AGCATGAGCA GCCATCCTAT 
GCTGAAGAGG ATAGTGCCCT CCAGGATGAC GATTACAATA ACGACAATGA CGATGACAAC 
GACGACAACA ACGAGAATGG AGCCGATGAG GATGATGAAG AAGATGACGA GGAGGATGTA 
CCATTGGCCA TGCGCAAACG AAAACAGGAA CCAATTGCTA ATAATGGGGA TGGCTATATG 
GATGATTATA ATGATGACAT TCCGCTGTTG GCGCGTAAGA AGATCAAAAA GGAGCCCAAA 
GAGAAGAGTC GTAAGCAAAA GAAGCGTCCC AAAGAAGAAC TTGATGATGA TTATGGCAGC 
ATGAAGCCCA AAATGAAAAA GGTTAAAAAG GAACCTGAGA TTAAGAGTGA AACTGTATCG 
CCCACCAAAC GTGCCCGTAA AGACAAAAAG GATGAGGAAG AAGAAGTCTG GAGATGGTGG 
GAAGAGGAGA AGCGTGAAGA TGGCGTCAAA TGGTCAACCC TAGAGCATAA GGGTCCTGTC 
TTTGCTCCAC CCTATGAGCG TGTGCCACGC AATGTACGCT TCTTTTACGA TGGCAAACCC 
TTGGATCTTA CCGAAGAAAC CGAAGAGGCA GCCACATTCT ATGCCAAAAT GTTAAATCAT 
GATTACTGCA CCAAGGATGT TTTCAATACC AATTTCTTCA AAGATTTCCG CAAAACCATG 
ACGGACAGGG AGAAAGAAAT TATCAAAGAT TTTCGTAAAT GCAATTTCCA AGAGATGTTT 
GAATTCTTTC AAGCCGAATC GGAGAGACGA AAGGCCGCCA CAAAGGATGA GAAACTGGCG 
AAAAAGAATG AGAACGAGGC ATTGGTTAAG GAATATGGCT TCTGTATGAT CGATGGTCAT 
AAAGAGAAAA TTGGTAATTT CCGTCTAGAG CCGCCGGGTC TATTTCGTGG TCGTGGTGAA 
CATCCGAAAA TGGGAATGAT CAAACGCCGC ATTGGGGCCT CTGAGGTATC GATTAATTGT 
GGTAAAAATT CCAAGGTACC AACACCACCA CCGGGCCAAC GTTGGAAGGA AGTACGTCAC 
GATAATACCG TCACTTGGCT AGCATCTTGG GTAGAGAATG TCCAGGGTCA AGTTAAATAT 
ATTATGTTGA ATCCATCGTC CAAGCTAAAG GGTGAAAAGG ATCACATTAA ATATGAGACA 
GCTCGCCGTT TGGATAAAGT GATTGATAAG ATTCGTGCCA CCTATCGTGA TGAATGGAAA 
TCTAAGGAGA TGCGTGTGCG TCAAAGAGCT GTTGCTTTGT ACTTTATCGA TAAATTAGCC 
CTGAGAGCGG GTAACGAAAA GGATGAGGAT CAGGCGGATA CTGTCGGCTG TTGTTCGTTG 
CGTGTCGAGC ATGTCCAACT GCATAGAGAG CTCAATGGCA AAGAGAATGT GGTTGTGTTC 
GATTTCCCTG GTAAGGATTC CATACGATAC TACAATGAGG TCGAGGTGGA GAAGCGTGTC 
TTTAAAAATC TCGAACTCTT TATGGAGAAT AAGAAGGAGG GCGATGATCT CTTCGATCGT 
CTCAATACCC AAGTTCTCAA CGAGCATCTT AAAGAGTTGA TGGATGGACT TACGGCTAAA 
GTATTTCGTA CATATAACGC TTCAAAAACG CTACAAGATC AATTGGATAA ACTAACCGAT 
GGCGATGCAA CAGTCGCTGA AAAATTGTTG GCCTATAATC GTGCCAATCG TGCTGTGGCC 
ATCCTTTGTA ACCATCAGCG TTCGGTGCCC AAGGGTCACC AAAAGTCCAT GGAGAATTTA 
AAAGAAAAAA TACGCGCCAA GCGCGATGCC ATTGATGATT GTGAATCTGA ATATCATGAT 
TTGAAAAAGG CGGCCAAACG TGGCTCGGTT AAAGAGAAAG TGAATGCCGA TAAGAAGGGC 
AAGCAGCTGG AACGTTTAAA GGAACAGCTA AAGAAGTTGG AACTACAAGA GACTGATAGA 
GACGAGAATA AGACCATTGC CTTGAGCACA TCCAAATTGA ATTATCTCGA TCCACGTATT 
TCGGTGGCCT GGTGTAAAAA GAATGGCGTG CCGATTGAAA AGATCTTTAA TAAAACGCAA 
AGAACCAAAT TTCTTTGGGC TATACATATG GCCGATGAGA ATTATCGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK25865 ( XM_002071529.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002071565.1 Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK25865), mRNA.

Price & Availability↑

CloneID	ODf11754
Clone ID Related Accession (Same CDS sequence)	XM_002071529.3 , XM_002071529.2
Accession Version	XM_002071529.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform A
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032860). On Dec 21, 2015 this sequence version replaced XM_002071529.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG ATGTGGCTGC TGAAAATAAC ATCCACATAC AAAATGGTGG AAGCTGCAGC 
GAAGTGCAAT CAAATGGTGT ATCGAATGGC CATCATAACC ACTACCATCA TAATAGCAGT 
AGCAGTAGCA GCAAACATAA ATCGTCTAGC AAGGATAAGC ATCGTGATAA GGAGAGAAGT 
GAGCATAAGA GTTCCAGTTC ATCCAAGGAG CATAAGAGTA GCAGTCGCGA CAAGGACAGG 
CATAAGAGCA GCAGCAGTGG CGGTAGCTCC TCGTCCAAGC ATAGGGATAA GGATCATCAT 
CGCAGTAGCT CCTCATCGTC AAGTCATCGT GATAAGGATG GAAGCAGCAG TAGCAGCAGC 
AAACACAAGT CCTCATCATC ATCATCGTCT GGCCACCATA AGAGCTCATC GAAGGATAAA 
GAACGCTCTA AGGACAAAGA TCGTAGCAAC AGTAGTAGCA GCAGCAGCAG CAGTAGCCGA 
GATAAGCGCC GCAGCGATCA CAAAAGCTCA TCTTCATCGA GCTCACGGCA CAGCAGTAGC 
TCCTCGCCAT CGAAAAGCAA ACATTCCAGT TCACGTCACA GTTCATCATC TGGCACTACT 
AGCAATTCTG GCAAGGATCC CTCCTATGAT GGTGTCTTTG TGAAACCGGA GCCAGTTTCT 
CAGCCCCCGA TGCAACAGCA TTATGCTGAA TGCTCAGCAG CCCCATCTGT AGCCAGCAAT 
GGTAATGGTG ATGATGGCAT GATTTACAAA AATGGCTACG AGGAGAACAT TGTCGATATC 
AAGAAGGAGG AGGACAACAG TTTCAATAAT CTATCACAGG CCAGTTCCTG TGACTACTCC 
TTGTCACAAT TTCGATCGGA TGAGCCATCG TTCGAGACAA AGCATGAGCA GCCATCCTAT 
GCTGAAGAGG ATAGTGCCCT CCAGGATGAC GATTACAATA ACGACAATGA CGATGACAAC 
GACGACAACA ACGAGAATGG AGCCGATGAG GATGATGAAG AAGATGACGA GGAGGATGTA 
CCATTGGCCA TGCGCAAACG AAAACAGGAA CCAATTGCTA ATAATGGGGA TGGCTATATG 
GATGATTATA ATGATGACAT TCCGCTGTTG GCGCGTAAGA AGATCAAAAA GGAGCCCAAA 
GAGAAGAGTC GTAAGCAAAA GAAGCGTCCC AAAGAAGAAC TTGATGATGA TTATGGCAGC 
ATGAAGCCCA AAATGAAAAA GGTTAAAAAG GAACCTGAGA TTAAGAGTGA AACTGTATCG 
CCCACCAAAC GTGCCCGTAA AGACAAAAAG GATGAGGAAG AAGAAGTCTG GAGATGGTGG 
GAAGAGGAGA AGCGTGAAGA TGGCGTCAAA TGGTCAACCC TAGAGCATAA GGGTCCTGTC 
TTTGCTCCAC CCTATGAGCG TGTGCCACGC AATGTACGCT TCTTTTACGA TGGCAAACCC 
TTGGATCTTA CCGAAGAAAC CGAAGAGGCA GCCACATTCT ATGCCAAAAT GTTAAATCAT 
GATTACTGCA CCAAGGATGT TTTCAATACC AATTTCTTCA AAGATTTCCG CAAAACCATG 
ACGGACAGGG AGAAAGAAAT TATCAAAGAT TTTCGTAAAT GCAATTTCCA AGAGATGTTT 
GAATTCTTTC AAGCCGAATC GGAGAGACGA AAGGCCGCCA CAAAGGATGA GAAACTGGCG 
AAAAAGAATG AGAACGAGGC ATTGGTTAAG GAATATGGCT TCTGTATGAT CGATGGTCAT 
AAAGAGAAAA TTGGTAATTT CCGTCTAGAG CCGCCGGGTC TATTTCGTGG TCGTGGTGAA 
CATCCGAAAA TGGGAATGAT CAAACGCCGC ATTGGGGCCT CTGAGGTATC GATTAATTGT 
GGTAAAAATT CCAAGGTACC AACACCACCA CCGGGCCAAC GTTGGAAGGA AGTACGTCAC 
GATAATACCG TCACTTGGCT AGCATCTTGG GTAGAGAATG TCCAGGGTCA AGTTAAATAT 
ATTATGTTGA ATCCATCGTC CAAGCTAAAG GGTGAAAAGG ATCACATTAA ATATGAGACA 
GCTCGCCGTT TGGATAAAGT GATTGATAAG ATTCGTGCCA CCTATCGTGA TGAATGGAAA 
TCTAAGGAGA TGCGTGTGCG TCAAAGAGCT GTTGCTTTGT ACTTTATCGA TAAATTAGCC 
CTGAGAGCGG GTAACGAAAA GGATGAGGAT CAGGCGGATA CTGTCGGCTG TTGTTCGTTG 
CGTGTCGAGC ATGTCCAACT GCATAGAGAG CTCAATGGCA AAGAGAATGT GGTTGTGTTC 
GATTTCCCTG GTAAGGATTC CATACGATAC TACAATGAGG TCGAGGTGGA GAAGCGTGTC 
TTTAAAAATC TCGAACTCTT TATGGAGAAT AAGAAGGAGG GCGATGATCT CTTCGATCGT 
CTCAATACCC AAGTTCTCAA CGAGCATCTT AAAGAGTTGA TGGATGGACT TACGGCTAAA 
GTATTTCGTA CATATAACGC TTCAAAAACG CTACAAGATC AATTGGATAA ACTAACCGAT 
GGCGATGCAA CAGTCGCTGA AAAATTGTTG GCCTATAATC GTGCCAATCG TGCTGTGGCC 
ATCCTTTGTA ACCATCAGCG TTCGGTGCCC AAGGGTCACC AAAAGTCCAT GGAGAATTTA 
AAAGAAAAAA TACGCGCCAA GCGCGATGCC ATTGATGATT GTGAATCTGA ATATCATGAT 
TTGAAAAAGG CGGCCAAACG TGGCTCGGTT AAAGAGAAAG TGAATGCCGA TAAGAAGGGC 
AAGCAGCTGG AACGTTTAAA GGAACAGCTA AAGAAGTTGG AACTACAAGA GACTGATAGA 
GACGAGAATA AGACCATTGC CTTGAGCACA TCCAAATTGA ATTATCTCGA TCCACGTATT 
TCGGTGGCCT GGTGTAAAAA GAATGGCGTG CCGATTGAAA AGATCTTTAA TAAAACGCAA 
AGAACCAAAT TTCTTTGGGC TATACATATG GCCGATGAGA ATTATCGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002071565.1
CDS	255..3248
Translation	MSGDVAAENNIHIQNGGSCSEVQSNGVSNGHHNHYHHNSSSSSSKHKSSSKDKHRDKERSEHKSSSSSKEHKSSSRDKDRHKSSSSGGSSSSKHRDKDHHRSSSSSSSHRDKDGSSSSSSKHKSSSSSSSGHHKSSSKDKERSKDKDRSNSSSSSSSSSRDKRRSDHKSSSSSSSRHSSSSSPSKSKHSSSRHSSSSGTTSNSGKDPSYDGVFVKPEPVSQPPMQQHYAECSAAPSVASNGNGDDGMIYKNGYEENIVDIKKEEDNSFNNLSQASSCDYSLSQFRSDEPSFETKHEQPSYAEEDSALQDDDYNNDNDDDNDDNNENGADEDDEEDDEEDVPLAMRKRKQEPIANNGDGYMDDYNDDIPLLARKKIKKEPKEKSRKQKKRPKEELDDDYGSMKPKMKKVKKEPEIKSETVSPTKRARKDKKDEEEEVWRWWEEEKREDGVKWSTLEHKGPVFAPPYERVPRNVRFFYDGKPLDLTEETEEAATFYAKMLNHDYCTKDVFNTNFFKDFRKTMTDREKEIIKDFRKCNFQEMFEFFQAESERRKAATKDEKLAKKNENEALVKEYGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIGASEVSINCGKNSKVPTPPPGQRWKEVRHDNTVTWLASWVENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHRELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMENKKEGDDLFDRLNTQVLNEHLKELMDGLTAKVFRTYNASKTLQDQLDKLTDGDATVAEKLLAYNRANRAVAILCNHQRSVPKGHQKSMENLKEKIRAKRDAIDDCESEYHDLKKAAKRGSVKEKVNADKKGKQLERLKEQLKKLELQETDRDENKTIALSTSKLNYLDPRISVAWCKKNGVPIEKIFNKTQRTKFLWAIHMADENYRF

Target ORF information:

RefSeq Version	XM_002071529.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant A (Dwil\GK25865), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002071529.2

ORF Insert Sequence:

ATGAGTGGGG ATGTGGCTGC TGAAAATAAC ATCCACATAC AAAATGGTGG AAGCTGCAGC 
GAAGTGCAAT CAAATGGTGT ATCGAATGGC CATCATAACC ACTACCATCA TAATAGCAGT 
AGCAGTAGCA GCAAACATAA ATCGTCTAGC AAGGATAAGC ATCGTGATAA GGAGAGAAGT 
GAGCATAAGA GTTCCAGTTC ATCCAAGGAG CATAAGAGTA GCAGTCGCGA CAAGGACAGG 
CATAAGAGCA GCAGCAGTGG CGGTAGCTCC TCGTCCAAGC ATAGGGATAA GGATCATCAT 
CGCAGTAGCT CCTCATCGTC AAGTCATCGT GATAAGGATG GAAGCAGCAG TAGCAGCAGC 
AAACACAAGT CCTCATCATC ATCATCGTCT GGCCACCATA AGAGCTCATC GAAGGATAAA 
GAACGCTCTA AGGACAAAGA TCGTAGCAAC AGTAGTAGCA GCAGCAGCAG CAGTAGCCGA 
GATAAGCGCC GCAGCGATCA CAAAAGCTCA TCTTCATCGA GCTCACGGCA CAGCAGTAGC 
TCCTCGCCAT CGAAAAGCAA ACATTCCAGT TCACGTCACA GTTCATCATC TGGCACTACT 
AGCAATTCTG GCAAGGATCC CTCCTATGAT GGTGTCTTTG TGAAACCGGA GCCAGTTTCT 
CAGCCCCCGA TGCAACAGCA TTATGCTGAA TGCTCAGCAG CCCCATCTGT AGCCAGCAAT 
GGTAATGGTG ATGATGGCAT GATTTACAAA AATGGCTACG AGGAGAACAT TGTCGATATC 
AAGAAGGAGG AGGACAACAG TTTCAATAAT CTATCACAGG CCAGTTCCTG TGACTACTCC 
TTGTCACAAT TTCGATCGGA TGAGCCATCG TTCGAGACAA AGCATGAGCA GCCATCCTAT 
GCTGAAGAGG ATAGTGCCCT CCAGGATGAC GATTACAATA ACGACAATGA CGATGACAAC 
GACGACAACA ACGAGAATGG AGCCGATGAG GATGATGAAG AAGATGACGA GGAGGATGTA 
CCATTGGCCA TGCGCAAACG AAAACAGGAA CCAATTGCTA ATAATGGGGA TGGCTATATG 
GATGATTATA ATGATGACAT TCCGCTGTTG GCGCGTAAGA AGATCAAAAA GGAGCCCAAA 
GAGAAGAGTC GTAAGCAAAA GAAGCGTCCC AAAGAAGAAC TTGATGATGA TTATGGCAGC 
ATGAAGCCCA AAATGAAAAA GGTTAAAAAG GAACCTGAGA TTAAGAGTGA AACTGTATCG 
CCCACCAAAC GTGCCCGTAA AGACAAAAAG GATGAGGAAG AAGAAGTCTG GAGATGGTGG 
GAAGAGGAGA AGCGTGAAGA TGGCGTCAAA TGGTCAACCC TAGAGCATAA GGGTCCTGTC 
TTTGCTCCAC CCTATGAGCG TGTGCCACGC AATGTACGCT TCTTTTACGA TGGCAAACCC 
TTGGATCTTA CCGAAGAAAC CGAAGAGGCA GCCACATTCT ATGCCAAAAT GTTAAATCAT 
GATTACTGCA CCAAGGATGT TTTCAATACC AATTTCTTCA AAGATTTCCG CAAAACCATG 
ACGGACAGGG AGAAAGAAAT TATCAAAGAT TTTCGTAAAT GCAATTTCCA AGAGATGTTT 
GAATTCTTTC AAGCCGAATC GGAGAGACGA AAGGCCGCCA CAAAGGATGA GAAACTGGCG 
AAAAAGAATG AGAACGAGGC ATTGGTTAAG GAATATGGCT TCTGTATGAT CGATGGTCAT 
AAAGAGAAAA TTGGTAATTT CCGTCTAGAG CCGCCGGGTC TATTTCGTGG TCGTGGTGAA 
CATCCGAAAA TGGGAATGAT CAAACGCCGC ATTGGGGCCT CTGAGGTATC GATTAATTGT 
GGTAAAAATT CCAAGGTACC AACACCACCA CCGGGCCAAC GTTGGAAGGA AGTACGTCAC 
GATAATACCG TCACTTGGCT AGCATCTTGG GTAGAGAATG TCCAGGGTCA AGTTAAATAT 
ATTATGTTGA ATCCATCGTC CAAGCTAAAG GGTGAAAAGG ATCACATTAA ATATGAGACA 
GCTCGCCGTT TGGATAAAGT GATTGATAAG ATTCGTGCCA CCTATCGTGA TGAATGGAAA 
TCTAAGGAGA TGCGTGTGCG TCAAAGAGCT GTTGCTTTGT ACTTTATCGA TAAATTAGCC 
CTGAGAGCGG GTAACGAAAA GGATGAGGAT CAGGCGGATA CTGTCGGCTG TTGTTCGTTG 
CGTGTCGAGC ATGTCCAACT GCATAGAGAG CTCAATGGCA AAGAGAATGT GGTTGTGTTC 
GATTTCCCTG GTAAGGATTC CATACGATAC TACAATGAGG TCGAGGTGGA GAAGCGTGTC 
TTTAAAAATC TCGAACTCTT TATGGAGAAT AAGAAGGAGG GCGATGATCT CTTCGATCGT 
CTCAATACCC AAGTTCTCAA CGAGCATCTT AAAGAGTTGA TGGATGGACT TACGGCTAAA 
GTATTTCGTA CATATAACGC TTCAAAAACG CTACAAGATC AATTGGATAA ACTAACCGAT 
GGCGATGCAA CAGTCGCTGA AAAATTGTTG GCCTATAATC GTGCCAATCG TGCTGTGGCC 
ATCCTTTGTA ACCATCAGCG TTCGGTGCCC AAGGGTCACC AAAAGTCCAT GGAGAATTTA 
AAAGAAAAAA TACGCGCCAA GCGCGATGCC ATTGATGATT GTGAATCTGA ATATCATGAT 
TTGAAAAAGG CGGCCAAACG TGGCTCGGTT AAAGAGAAAG TGAATGCCGA TAAGAAGGGC 
AAGCAGCTGG AACGTTTAAA GGAACAGCTA AAGAAGTTGG AACTACAAGA GACTGATAGA 
GACGAGAATA AGACCATTGC CTTGAGCACA TCCAAATTGA ATTATCTCGA TCCACGTATT 
TCGGTGGCCT GGTGTAAAAA GAATGGCGTG CCGATTGAAA AGATCTTTAA TAAAACGCAA 
AGAACCAAAT TTCTTTGGGC TATACATATG GCCGATGAGA ATTATCGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK25865 ( XM_015177230.1 ) cDNA ORF clone, Drosophila willistoni -> XP_015032716.1 Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK25865), mRNA.

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CloneID	ODf17229
Clone ID Related Accession (Same CDS sequence)	XM_015177230.1
Accession Version	XM_015177230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein, isoform B
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032860). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAATC ATGATTACTG CACCAAGGAT GTTTTCAATA CCAATTTCTT CAAAGATTTC 
CGCAAAACCA TGACGGACAG GGAGAAAGAA ATTATCAAAG ATTTTCGTAA ATGCAATTTC 
CAAGAGATGT TTGAATTCTT TCAAGCCGAA TCGGAGAGAC GAAAGGCCGC CACAAAGGAT 
GAGAAACTGG CGAAAAAGAA TGAGAACGAG GCATTGGTTA AGGAATATGG CTTCTGTATG 
ATCGATGGTC ATAAAGAGAA AATTGGTAAT TTCCGTCTAG AGCCGCCGGG TCTATTTCGT 
GGTCGTGGTG AACATCCGAA AATGGGAATG ATCAAACGCC GCATTGGGGC CTCTGAGGTA 
TCGATTAATT GTGGTAAAAA TTCCAAGGTA CCAACACCAC CACCGGGCCA ACGTTGGAAG 
GAAGTACGTC ACGATAATAC CGTCACTTGG CTAGCATCTT GGGTAGAGAA TGTCCAGGGT 
CAAGTTAAAT ATATTATGTT GAATCCATCG TCCAAGCTAA AGGGTGAAAA GGATCACATT 
AAATATGAGA CAGCTCGCCG TTTGGATAAA GTGATTGATA AGATTCGTGC CACCTATCGT 
GATGAATGGA AATCTAAGGA GATGCGTGTG CGTCAAAGAG CTGTTGCTTT GTACTTTATC 
GATAAATTAG CCCTGAGAGC GGGTAACGAA AAGGATGAGG ATCAGGCGGA TACTGTCGGC 
TGTTGTTCGT TGCGTGTCGA GCATGTCCAA CTGCATAGAG AGCTCAATGG CAAAGAGAAT 
GTGGTTGTGT TCGATTTCCC TGGTAAGGAT TCCATACGAT ACTACAATGA GGTCGAGGTG 
GAGAAGCGTG TCTTTAAAAA TCTCGAACTC TTTATGGAGA ATAAGAAGGA GGGCGATGAT 
CTCTTCGATC GTCTCAATAC CCAAGTTCTC AACGAGCATC TTAAAGAGTT GATGGATGGA 
CTTACGGCTA AAGTATTTCG TACATATAAC GCTTCAAAAA CGCTACAAGA TCAATTGGAT 
AAACTAACCG ATGGCGATGC AACAGTCGCT GAAAAATTGT TGGCCTATAA TCGTGCCAAT 
CGTGCTGTGG CCATCCTTTG TAACCATCAG CGTTCGGTGC CCAAGGGTCA CCAAAAGTCC 
ATGGAGAATT TAAAAGAAAA AATACGCGCC AAGCGCGATG CCATTGATGA TTGTGAATCT 
GAATATCATG ATTTGAAAAA GGCGGCCAAA CGTGGCTCGG TTAAAGAGAA AGTGAATGCC 
GATAAGAAGG GCAAGCAGCT GGAACGTTTA AAGGAACAGC TAAAGAAGTT GGAACTACAA 
GAGACTGATA GAGACGAGAA TAAGACCATT GCCTTGAGCA CATCCAAATT GAATTATCTC 
GATCCACGTA TTTCGGTGGC CTGGTGTAAA AAGAATGGCG TGCCGATTGA AAAGATCTTT 
AATAAAACGC AAAGAACCAA ATTTCTTTGG GCTATACATA TGGCCGATGA GAATTATCGT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015032716.1
CDS	293..1798
Translation	MLNHDYCTKDVFNTNFFKDFRKTMTDREKEIIKDFRKCNFQEMFEFFQAESERRKAATKDEKLAKKNENEALVKEYGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIGASEVSINCGKNSKVPTPPPGQRWKEVRHDNTVTWLASWVENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHRELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMENKKEGDDLFDRLNTQVLNEHLKELMDGLTAKVFRTYNASKTLQDQLDKLTDGDATVAEKLLAYNRANRAVAILCNHQRSVPKGHQKSMENLKEKIRAKRDAIDDCESEYHDLKKAAKRGSVKEKVNADKKGKQLERLKEQLKKLELQETDRDENKTIALSTSKLNYLDPRISVAWCKKNGVPIEKIFNKTQRTKFLWAIHMADENYRF

Target ORF information:

RefSeq Version	XM_015177230.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein, transcript variant B (Dwil\GK25865), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015177230.1

ORF Insert Sequence:

ATGTTAAATC ATGATTACTG CACCAAGGAT GTTTTCAATA CCAATTTCTT CAAAGATTTC 
CGCAAAACCA TGACGGACAG GGAGAAAGAA ATTATCAAAG ATTTTCGTAA ATGCAATTTC 
CAAGAGATGT TTGAATTCTT TCAAGCCGAA TCGGAGAGAC GAAAGGCCGC CACAAAGGAT 
GAGAAACTGG CGAAAAAGAA TGAGAACGAG GCATTGGTTA AGGAATATGG CTTCTGTATG 
ATCGATGGTC ATAAAGAGAA AATTGGTAAT TTCCGTCTAG AGCCGCCGGG TCTATTTCGT 
GGTCGTGGTG AACATCCGAA AATGGGAATG ATCAAACGCC GCATTGGGGC CTCTGAGGTA 
TCGATTAATT GTGGTAAAAA TTCCAAGGTA CCAACACCAC CACCGGGCCA ACGTTGGAAG 
GAAGTACGTC ACGATAATAC CGTCACTTGG CTAGCATCTT GGGTAGAGAA TGTCCAGGGT 
CAAGTTAAAT ATATTATGTT GAATCCATCG TCCAAGCTAA AGGGTGAAAA GGATCACATT 
AAATATGAGA CAGCTCGCCG TTTGGATAAA GTGATTGATA AGATTCGTGC CACCTATCGT 
GATGAATGGA AATCTAAGGA GATGCGTGTG CGTCAAAGAG CTGTTGCTTT GTACTTTATC 
GATAAATTAG CCCTGAGAGC GGGTAACGAA AAGGATGAGG ATCAGGCGGA TACTGTCGGC 
TGTTGTTCGT TGCGTGTCGA GCATGTCCAA CTGCATAGAG AGCTCAATGG CAAAGAGAAT 
GTGGTTGTGT TCGATTTCCC TGGTAAGGAT TCCATACGAT ACTACAATGA GGTCGAGGTG 
GAGAAGCGTG TCTTTAAAAA TCTCGAACTC TTTATGGAGA ATAAGAAGGA GGGCGATGAT 
CTCTTCGATC GTCTCAATAC CCAAGTTCTC AACGAGCATC TTAAAGAGTT GATGGATGGA 
CTTACGGCTA AAGTATTTCG TACATATAAC GCTTCAAAAA CGCTACAAGA TCAATTGGAT 
AAACTAACCG ATGGCGATGC AACAGTCGCT GAAAAATTGT TGGCCTATAA TCGTGCCAAT 
CGTGCTGTGG CCATCCTTTG TAACCATCAG CGTTCGGTGC CCAAGGGTCA CCAAAAGTCC 
ATGGAGAATT TAAAAGAAAA AATACGCGCC AAGCGCGATG CCATTGATGA TTGTGAATCT 
GAATATCATG ATTTGAAAAA GGCGGCCAAA CGTGGCTCGG TTAAAGAGAA AGTGAATGCC 
GATAAGAAGG GCAAGCAGCT GGAACGTTTA AAGGAACAGC TAAAGAAGTT GGAACTACAA 
GAGACTGATA GAGACGAGAA TAAGACCATT GCCTTGAGCA CATCCAAATT GAATTATCTC 
GATCCACGTA TTTCGGTGGC CTGGTGTAAA AAGAATGGCG TGCCGATTGA AAAGATCTTT 
AATAAAACGC AAAGAACCAA ATTTCTTTGG GCTATACATA TGGCCGATGA GAATTATCGT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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DWIL\GK25865 cDNA ORF clone, Drosophila willistoni

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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