DWIL\GK20459 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DWIL\GK20459
Entrez Gene ID	6642921
Full Name	2-oxoglutarate dehydrogenase, mitochondrial
Synonyms	GK20459,GK20459-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_023176245.1	XP_023032013.1	2-oxoglutarate dehydrogenase, mitochondrial
XM_002065824.2	XP_002065860.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DWIL\GK20459 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWIL\GK20459 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODf05032	XM_023176245.1 Latest version!	Drosophila willistoni 2-oxoglutarate dehydrogenase, mitochondrial (LOC6642921), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODf19575	XM_002065824.2 Latest version!	Drosophila willistoni uncharacterized protein (Dwil\GK20459), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWIL\GK20459 ( XM_023176245.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023032013.1 Drosophila willistoni 2-oxoglutarate dehydrogenase, mitochondrial (LOC6642921), mRNA.

Price & Availability↑

CloneID	ODf05032
Clone ID Related Accession (Same CDS sequence)	XM_023176245.1
Accession Version	XM_023176245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	2-oxoglutarate dehydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG GCCGCGTTAA ACATCTAATG TTGGTGGTGA ATTTTTCGAA AATTGATGTT 
GCTCCCAGGC TTTTAAGGGA GTATGCACGT TACCATTATC GGAAACTGCA AACGTCCAGT 
TCAGTTTGCA AGAGTAGCCA GGATAATACC AAGGACACGG ATAGTTTTGC GAATGGCAGC 
AGTGCCGCCT ATATAGAAGG TCTATATACC AAATGGTCGA AAAACCCCAG ATCAGTGGAT 
TCGTCATGGG ATGCCTATTT CAGTGGCAAG CCACGTAGTA TATCCACTAC ACCGTCACTG 
TACAAGAAGT CCGAAGTGAG TGCAGATTCG TCGGCAACTA AAACAACAAA AGATGCGGCA 
GCAGCAGCCC CGCCGCCTGC GTCAGCGGGT GGAGTAGCAC CAGCAGCAGC AGCAGGAGCA 
CCACCGGCAG CTGACTGGAA ATACATAGAT GATCATCTCA CAGTCCAGGC TATTATCCGA 
GCGTATCAGG TGCGAGGACA TTTAGCTGCC GATCTGGATC CTTTGGGTAT TGTTGGACCA 
CCAGGCATTA CCTCGGTAGA CGGCAAGAAA CGAAATGCCG CTCAAGCGGT ACTGCGCCAG 
CATTATACCT ATGTATTTAA TGATCTCAAT GCCACATTTA AATTACCATC TTCCACCATG 
ATCGGCGGTG ATGAGCAGTT TCTGCCTTTG AAGGAAATTT TAGATCGTCT GGAGCGAGTG 
TATTGTGGTC ACATTGGAGT GGAGTACATG CAAATTACGT CTTTGACAAA AACCAACTGG 
ATACGCGAAA GATTTGAGAA GCCTGGTGCC ATAATGTTTA ATCCTGATGA AAAGAAGTTA 
ATTCTAGAGC GCCTCACGCG TTCCACGGGA TTCGAAAACT TTTTGGCCAA GAAGTTTACG 
TCGGAGAAAC GTTTCGGTTT AGAGGGTTGC GACATAATGA TACCCATCAT GAAGGAGATC 
ATCGATAGAT CGACCAAATT AGGAGTAGAG TCCATATTTA TAGGCATGGC GCATCGCGGC 
CGTCTTAATG TTCTGGCCAA TATTTGTCGT AAGCCTATTC CTGATATTTT GTCACAATTC 
CATGGCCTTA AGGCCACCGA TTCGGGATCT GGCGATGTGA AATACCATCT TGGTCTATTT 
ACGGAACGTC TTAATCGAGT GACAAATAAG AATGTGCGCA TAACTGTGGT CGCCAATCCT 
TCACATTTGG AGTATGTGAA TCCGGTGGTG TTGGGCAAGG CACGTGCTGA AATGTATCAA 
CGTGGTGATA GCAATGGTAG TAAGGTTCTC TCCATTATCA TACACGGCGA CGCTTCCTTC 
TGCGGCCAGG GTGTGGTGTA CGAGTCAATG CATCTATCGG ATTTGCCATG CTACACAACT 
TTTGGCACCA TTCATATAGT GGCAAACAAC CAGGTGGGTT TCACAACAGA TCCACGTTTC 
TCGCGCTCCT CACGTTACTG CACGGATGTC GCTCGTGTGG TCAATGCCCC AATCTTTCAT 
GTGAACGCCG ATGATCCTGA GGCATGCATA CACTGTGCTC GCGTCTCTAC CGACTATCGG 
GCCGAATTCC ACAAAGATGT CGTCATTGAC ATTGTGGGCT ATCGTCGTAA TGGTCATAAT 
GAAGCTGATG AACCCATGTT CACTCAACCC CTCATGTATC AGCGCATAAG AAAGCTTCAA 
CCAGTTATGC AAAAATATGC CGAGAAGCTA ATCAAAGAGG GGGTCGTTCA GGAGAAAGAA 
TATAAGGACA TGATCGCAAA ATATGAGAAA ATATGCGACG ACGCTTTCAT CGAGTCTAAG 
AAAGTGAAAA CCATCAAGTA TTCTTCGTGG ATCGATTCGC CGTGGACGGG CTTTTTTGAA 
GGGCGAGATC GTTTGAAAGT TTGTCCCACT GGAGTTAGCT TGGAAACCCT TAATAATATT 
GGTGAGATCT TCTCGAATCC GCCGCCTGCC GAGCACAAGT TTGAGGTGCA CAAGGGAGTC 
CTTCGTATAT TGGCCTTGCG CAAGCAAATG GTAAAATCGC AAATGGCTGA CTGGTCTCTG 
GGTGAGGCTT TTGCCATTGG CACTTTACTG AAGGATGGCA TTCATGTGCG ACTATCTGGC 
CAGGATGTGG AGCGGGGAAC CTTCTCCCAT CGACATCATG TCTTCCATCA TCAAAGTCAG 
GACAAGGGTC GATATAATTC CATTGAGCAT CTATATCCCG ATCAGTCACC TTATGTTGTT 
TGCAATAGCT CCTTATCGGA GTGTGCCATA ATGGGCTTTG AGCATGGCTA CTCGATGGCC 
CATCCCAATA CACTGGTCAT ATGGGAGGCG CAGTTTGGTG ATTTTGCGAA CACAGCCCAA 
ACAATTATCG ATTCCTTTAT AGCCAGTGGT GAAACGAAAT GGGTTCGCCA ATCTGGGCTT 
GTTCTTCTGC TGCCGCATTC CATGGAGGGT ATGGGGCCAG AGCATTCCTC CGGTCGCATT 
GAACGCTTTT TGCAGCTAAG CGATGACGAT CCAGACATCT ATCCCGATAC TTGTGATTGT 
GATTTCGTAG CTCGCCAACT AATGGACACG AATTGGATTG TGACAAATCT ATCGACGCCT 
GCCAATCTCT TTCATGCTTT GCGCCGTCAA GTGGCCTTAG GTTTTCGTAA ACCGCTAATT 
AATTTTTCAC CAAAATCTGT TTTGCGTCAC CCATTGGCCC GGAGTCCATT CCGTGATTTT 
ACCGAATGCA GTGAATTTAT GCGAATAATA CCCGAAACCG GCAAAGCAGG CTGTAAACCT 
GATTGTGTGG AAAAGGTTGT CTTTTGTTCA GGAAAGGTCT ACTACGATTT GGTCAAGGAG 
CGTGAGGAAC ACGAGCAAGA CGAAACTGTG GCCATTATTC GCGTAGAACA GGTCTGTCCG 
TTTCCATATG ATTTGATAAC CAAACAAATT GAACTATATA AGAATGCCGA ATTGATTTGG 
TGTCAGGAAG AGCATAAAAA TCAAGGATCT TGGTCGTATG TCCAGCAACG TTTCGATACC 
ACGCTAATCA AAATGGAAGA CGAAACCCGG TGTATATCCT ATCATGGACG TCCGCCAAAT 
TCGGCGCCAT CTACTGGCAA TAAGGTTCGG CATTATACTG AATACAAGAA TTTCATGACC 
AGCATTTTTG GTGAACTCAC CGAGGAAAAT AAACAGCGTC TTGAGCGGAT TGTCAAAGAA 
CAGCAAGCGC AGGCTAAGCA GGCCAGTAGT AGCACTAAAG CAAAGACTGT GGGCCAAGGC 
GAAGACAAAA CGAAAGCCAA ATCCAGTAAA GACGACAAGA AGGACGATAA GAAAGACGAC 
AAGAAGGACG ATAAGAAAGA CGATAAGAAG GCTAAACCTG GAGCAACAAC CGCTCCTCCC 
ACTTCTCCTC CAGCATCACC ACCACCGAAA CAAGATAATA AATCTCCAAC AACGTCGGCG 
CCCAAAGGTG ACATGAATCC AGCTGATAAA ACTAAAGGCA AGATTGACAC CAAACAAGAA 
AATTCTAAAA AAGCCACTAC AGACGAAACC AATAAAAATG ACGAGAACCT CAAGAAACCG 
ACAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023032013.1
CDS	31..3579
Translation	MNQGRVKHLMLVVNFSKIDVAPRLLREYARYHYRKLQTSSSVCKSSQDNTKDTDSFANGSSAAYIEGLYTKWSKNPRSVDSSWDAYFSGKPRSISTTPSLYKKSEVSADSSATKTTKDAAAAAPPPASAGGVAPAAAAGAPPAADWKYIDDHLTVQAIIRAYQVRGHLAADLDPLGIVGPPGITSVDGKKRNAAQAVLRQHYTYVFNDLNATFKLPSSTMIGGDEQFLPLKEILDRLERVYCGHIGVEYMQITSLTKTNWIRERFEKPGAIMFNPDEKKLILERLTRSTGFENFLAKKFTSEKRFGLEGCDIMIPIMKEIIDRSTKLGVESIFIGMAHRGRLNVLANICRKPIPDILSQFHGLKATDSGSGDVKYHLGLFTERLNRVTNKNVRITVVANPSHLEYVNPVVLGKARAEMYQRGDSNGSKVLSIIIHGDASFCGQGVVYESMHLSDLPCYTTFGTIHIVANNQVGFTTDPRFSRSSRYCTDVARVVNAPIFHVNADDPEACIHCARVSTDYRAEFHKDVVIDIVGYRRNGHNEADEPMFTQPLMYQRIRKLQPVMQKYAEKLIKEGVVQEKEYKDMIAKYEKICDDAFIESKKVKTIKYSSWIDSPWTGFFEGRDRLKVCPTGVSLETLNNIGEIFSNPPPAEHKFEVHKGVLRILALRKQMVKSQMADWSLGEAFAIGTLLKDGIHVRLSGQDVERGTFSHRHHVFHHQSQDKGRYNSIEHLYPDQSPYVVCNSSLSECAIMGFEHGYSMAHPNTLVIWEAQFGDFANTAQTIIDSFIASGETKWVRQSGLVLLLPHSMEGMGPEHSSGRIERFLQLSDDDPDIYPDTCDCDFVARQLMDTNWIVTNLSTPANLFHALRRQVALGFRKPLINFSPKSVLRHPLARSPFRDFTECSEFMRIIPETGKAGCKPDCVEKVVFCSGKVYYDLVKEREEHEQDETVAIIRVEQVCPFPYDLITKQIELYKNAELIWCQEEHKNQGSWSYVQQRFDTTLIKMEDETRCISYHGRPPNSAPSTGNKVRHYTEYKNFMTSIFGELTEENKQRLERIVKEQQAQAKQASSSTKAKTVGQGEDKTKAKSSKDDKKDDKKDDKKDDKKDDKKAKPGATTAPPTSPPASPPPKQDNKSPTTSAPKGDMNPADKTKGKIDTKQENSKKATTDETNKNDENLKKPTK

Target ORF information:

RefSeq Version	XM_023176245.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni 2-oxoglutarate dehydrogenase, mitochondrial (LOC6642921), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023176245.1

ORF Insert Sequence:

ATGAACCAGG GCCGCGTTAA ACATCTAATG TTGGTGGTGA ATTTTTCGAA AATTGATGTT 
GCTCCCAGGC TTTTAAGGGA GTATGCACGT TACCATTATC GGAAACTGCA AACGTCCAGT 
TCAGTTTGCA AGAGTAGCCA GGATAATACC AAGGACACGG ATAGTTTTGC GAATGGCAGC 
AGTGCCGCCT ATATAGAAGG TCTATATACC AAATGGTCGA AAAACCCCAG ATCAGTGGAT 
TCGTCATGGG ATGCCTATTT CAGTGGCAAG CCACGTAGTA TATCCACTAC ACCGTCACTG 
TACAAGAAGT CCGAAGTGAG TGCAGATTCG TCGGCAACTA AAACAACAAA AGATGCGGCA 
GCAGCAGCCC CGCCGCCTGC GTCAGCGGGT GGAGTAGCAC CAGCAGCAGC AGCAGGAGCA 
CCACCGGCAG CTGACTGGAA ATACATAGAT GATCATCTCA CAGTCCAGGC TATTATCCGA 
GCGTATCAGG TGCGAGGACA TTTAGCTGCC GATCTGGATC CTTTGGGTAT TGTTGGACCA 
CCAGGCATTA CCTCGGTAGA CGGCAAGAAA CGAAATGCCG CTCAAGCGGT ACTGCGCCAG 
CATTATACCT ATGTATTTAA TGATCTCAAT GCCACATTTA AATTACCATC TTCCACCATG 
ATCGGCGGTG ATGAGCAGTT TCTGCCTTTG AAGGAAATTT TAGATCGTCT GGAGCGAGTG 
TATTGTGGTC ACATTGGAGT GGAGTACATG CAAATTACGT CTTTGACAAA AACCAACTGG 
ATACGCGAAA GATTTGAGAA GCCTGGTGCC ATAATGTTTA ATCCTGATGA AAAGAAGTTA 
ATTCTAGAGC GCCTCACGCG TTCCACGGGA TTCGAAAACT TTTTGGCCAA GAAGTTTACG 
TCGGAGAAAC GTTTCGGTTT AGAGGGTTGC GACATAATGA TACCCATCAT GAAGGAGATC 
ATCGATAGAT CGACCAAATT AGGAGTAGAG TCCATATTTA TAGGCATGGC GCATCGCGGC 
CGTCTTAATG TTCTGGCCAA TATTTGTCGT AAGCCTATTC CTGATATTTT GTCACAATTC 
CATGGCCTTA AGGCCACCGA TTCGGGATCT GGCGATGTGA AATACCATCT TGGTCTATTT 
ACGGAACGTC TTAATCGAGT GACAAATAAG AATGTGCGCA TAACTGTGGT CGCCAATCCT 
TCACATTTGG AGTATGTGAA TCCGGTGGTG TTGGGCAAGG CACGTGCTGA AATGTATCAA 
CGTGGTGATA GCAATGGTAG TAAGGTTCTC TCCATTATCA TACACGGCGA CGCTTCCTTC 
TGCGGCCAGG GTGTGGTGTA CGAGTCAATG CATCTATCGG ATTTGCCATG CTACACAACT 
TTTGGCACCA TTCATATAGT GGCAAACAAC CAGGTGGGTT TCACAACAGA TCCACGTTTC 
TCGCGCTCCT CACGTTACTG CACGGATGTC GCTCGTGTGG TCAATGCCCC AATCTTTCAT 
GTGAACGCCG ATGATCCTGA GGCATGCATA CACTGTGCTC GCGTCTCTAC CGACTATCGG 
GCCGAATTCC ACAAAGATGT CGTCATTGAC ATTGTGGGCT ATCGTCGTAA TGGTCATAAT 
GAAGCTGATG AACCCATGTT CACTCAACCC CTCATGTATC AGCGCATAAG AAAGCTTCAA 
CCAGTTATGC AAAAATATGC CGAGAAGCTA ATCAAAGAGG GGGTCGTTCA GGAGAAAGAA 
TATAAGGACA TGATCGCAAA ATATGAGAAA ATATGCGACG ACGCTTTCAT CGAGTCTAAG 
AAAGTGAAAA CCATCAAGTA TTCTTCGTGG ATCGATTCGC CGTGGACGGG CTTTTTTGAA 
GGGCGAGATC GTTTGAAAGT TTGTCCCACT GGAGTTAGCT TGGAAACCCT TAATAATATT 
GGTGAGATCT TCTCGAATCC GCCGCCTGCC GAGCACAAGT TTGAGGTGCA CAAGGGAGTC 
CTTCGTATAT TGGCCTTGCG CAAGCAAATG GTAAAATCGC AAATGGCTGA CTGGTCTCTG 
GGTGAGGCTT TTGCCATTGG CACTTTACTG AAGGATGGCA TTCATGTGCG ACTATCTGGC 
CAGGATGTGG AGCGGGGAAC CTTCTCCCAT CGACATCATG TCTTCCATCA TCAAAGTCAG 
GACAAGGGTC GATATAATTC CATTGAGCAT CTATATCCCG ATCAGTCACC TTATGTTGTT 
TGCAATAGCT CCTTATCGGA GTGTGCCATA ATGGGCTTTG AGCATGGCTA CTCGATGGCC 
CATCCCAATA CACTGGTCAT ATGGGAGGCG CAGTTTGGTG ATTTTGCGAA CACAGCCCAA 
ACAATTATCG ATTCCTTTAT AGCCAGTGGT GAAACGAAAT GGGTTCGCCA ATCTGGGCTT 
GTTCTTCTGC TGCCGCATTC CATGGAGGGT ATGGGGCCAG AGCATTCCTC CGGTCGCATT 
GAACGCTTTT TGCAGCTAAG CGATGACGAT CCAGACATCT ATCCCGATAC TTGTGATTGT 
GATTTCGTAG CTCGCCAACT AATGGACACG AATTGGATTG TGACAAATCT ATCGACGCCT 
GCCAATCTCT TTCATGCTTT GCGCCGTCAA GTGGCCTTAG GTTTTCGTAA ACCGCTAATT 
AATTTTTCAC CAAAATCTGT TTTGCGTCAC CCATTGGCCC GGAGTCCATT CCGTGATTTT 
ACCGAATGCA GTGAATTTAT GCGAATAATA CCCGAAACCG GCAAAGCAGG CTGTAAACCT 
GATTGTGTGG AAAAGGTTGT CTTTTGTTCA GGAAAGGTCT ACTACGATTT GGTCAAGGAG 
CGTGAGGAAC ACGAGCAAGA CGAAACTGTG GCCATTATTC GCGTAGAACA GGTCTGTCCG 
TTTCCATATG ATTTGATAAC CAAACAAATT GAACTATATA AGAATGCCGA ATTGATTTGG 
TGTCAGGAAG AGCATAAAAA TCAAGGATCT TGGTCGTATG TCCAGCAACG TTTCGATACC 
ACGCTAATCA AAATGGAAGA CGAAACCCGG TGTATATCCT ATCATGGACG TCCGCCAAAT 
TCGGCGCCAT CTACTGGCAA TAAGGTTCGG CATTATACTG AATACAAGAA TTTCATGACC 
AGCATTTTTG GTGAACTCAC CGAGGAAAAT AAACAGCGTC TTGAGCGGAT TGTCAAAGAA 
CAGCAAGCGC AGGCTAAGCA GGCCAGTAGT AGCACTAAAG CAAAGACTGT GGGCCAAGGC 
GAAGACAAAA CGAAAGCCAA ATCCAGTAAA GACGACAAGA AGGACGATAA GAAAGACGAC 
AAGAAGGACG ATAAGAAAGA CGATAAGAAG GCTAAACCTG GAGCAACAAC CGCTCCTCCC 
ACTTCTCCTC CAGCATCACC ACCACCGAAA CAAGATAATA AATCTCCAAC AACGTCGGCG 
CCCAAAGGTG ACATGAATCC AGCTGATAAA ACTAAAGGCA AGATTGACAC CAAACAAGAA 
AATTCTAAAA AAGCCACTAC AGACGAAACC AATAAAAATG ACGAGAACCT CAAGAAACCG 
ACAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK20459 ( XM_002065824.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002065860.2 Drosophila willistoni uncharacterized protein (Dwil\GK20459), partial mRNA.

Price & Availability↑

CloneID	ODf19575
Clone ID Related Accession (Same CDS sequence)	XM_002065824.2
Accession Version	XM_002065824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032497). On Dec 21, 2015 this sequence version replaced XM_002065824.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGTTGGTGG TGAATTTTTC GAAAATTGAT GTTGCTCCCA GGCTTTTAAG GGAGTATGCA 
CGTTACCATT ATCGGAAACT GCAAACGTCC AGTTCAGTTT GCAAGAGTAG CCAGGATAAT 
ACCAAGGACA CGGATAGTTT TGCGAATGGC AGCAGTGCCG CCTATATAGA AGGTCTATAT 
ACCAAATGGT CGAAAAACCC CAGATCAGTG GATTCGTCAT GGGATGCCTA TTTCAGTGGC 
AAGCCACGTA GTATATCCAC TACACCGTCA CTGTACAAGA AGTCCGAAGT GAGTGCAGAT 
TCGTCGGCAA CTAAAACAAC AAAAGCAGCA GCAGGAGCAC CACCGGCAGC TGACTGGAAA 
TACATAGATG ATCATCTCAC AGTCCAGGCT ATTATCCGAG CGTATCAGGT GCGAGGACAT 
TTAGCTGCCG ATCTGGATCC TTTGGGTATT GTTGGACCAC CAGGCATTAC CTCGGTAGAC 
GGCAAGAAAC GAAATGCCGC TCAAGCGGTA CTGCGCCAGC ATTATACCTA TGTATTTAAT 
GATCTCAATG CCACATTTAA ATTACCATCT TCCACCATGA TCGGCGGTGA TGAGCAGTTT 
CTGCCTTTGA AGGAAATTTT AGATCGTCTG GAGCGAGTGT ATTGTGGTCA CATTGGAGTG 
GAGTACATGC AAATTACGTC TTTGACAAAA ACCAACTGGA TACGCGAAAG ATTTGAGAAG 
CCTGGTGCCA TAATGTTTAA TCCTGATGAA AAGAAGTTAA TTCTAGAGCG CCTCACGCGT 
TCCACGGGAT TCGAAAACTT TTTGGCCAAG AAGTTTACGT CGGAGAAACG TTTCGGTTTA 
GAGGGTTGCG ACATAATGAT ACCCATCATG AAGGAGATCA TCGATAGATC GACCAAATTA 
GGAGTAGAGT CCATATTTAT AGGCATGGCG CATCGCGGCC GTCTTAATGT TCTGGCCAAT 
ATTTGTCGTA AGCCTATTCC TGATATTTTG TCACAATTCC ATGGCCTTAA GGCCACCGAT 
TCGGGATCTG GCGATGTGAA ATACCATCTT GGTCTATTTA CGGAACGTCT TAATCGAGTG 
ACAAATAAGA ATGTGCGCAT AACTGTGGTC GCCAATCCTT CACATTTGGA GTATGTGAAT 
CCGGTGGTGT TGGGCAAGGC ACGTGCTGAA ATGTATCAAC GTGGTGATAG CAATGGTAGT 
AAGGTTCTCT CCATTATCAT ACACGGCGAC GCTTCCTTCT GCGGCCAGGG TGTGGTGTAC 
GAGTCAATGC ATCTATCGGA TTTGCCATGC TACACAACTT TTGGCACCAT TCATATAGTG 
GCAAACAACC AGGTGGGTTT CACAACAGAT CCACGTTTCT CGCGCTCCTC ACGTTACTGC 
ACGGATGTCG CTCGTGTGGT CAATGCCCCA ATCTTTCATG TGAACGCCGA TGATCCTGAG 
GCATGCATAC ACTGTGCTCG CGTCTCTACC GACTATCGGG CCGAATTCCA CAAAGATGTC 
GTCATTGACA TTGTGGGCTA TCGTCGTAAT GGTCATAATG AAGCTGATGA ACCCATGTTC 
ACTCAACCCC TCATGTATCA GCGCATAAGA AAGCTTCAAC CAGTTATGCA AAAATATGCC 
GAGAAGCTAA TCAAAGAGGG GGTCGTTCAG GAGAAAGAAT ATAAGGACAT GATCGCAAAA 
TATGAGAAAA TATGCGACGA CGCTTTCATC GAGTCTAAGA AAGTGAAAAC CATCAAGTAT 
TCTTCGTGGA TCGATTCGCC GTGGACGGGC TTTTTTGAAG GGCGAGATCG TTTGAAAGTT 
TGTCCCACTG GAGTTAGCTT GGAAACCCTT AATAATATTG GTGAGATCTT CTCGAATCCG 
CCGCCTGCCG AGCACAAGTT TGAGGTGCAC AAGGGAGTCC TTCGTATATT GGCCTTGCGC 
AAGCAAATGG TAAAATCGCA AATGGCTGAC TGGTCTCTGG GTGAGGCTTT TGCCATTGGC 
ACTTTACTGA AGGATGGCAT TCATGTGCGA CTATCTGGCC AGGATGTGGA GCGGGGAACC 
TTCTCCCATC GACATCATGT CTTCCATCAT CAAAGTCAGG ACAAGGGTCG ATATAATTCC 
ATTGAGCATC TATATCCCGA TCAGTCACCT TATGTTGTTT GCAATAGCTC CTTATCGGAG 
TGTGCCATAA TGGGCTTTGA GCATGGCTAC TCGATGGCCC ATCCCAATAC ACTGGTCATA 
TGGGAGGCGC AGTTTGGTGA TTTTGCGAAC ACAGCCCAAA CAATTATCGA TTCCTTTATA 
GCCAGTGGTG AAACGAAATG GGTTCGCCAA TCTGGGCTTG TTCTTCTGCT GCCGCATTCC 
ATGGAGGGTA TGGGGCCAGA GCATTCCTCC GGTCGCATTG AACGCTTTTT GCAGCTAAGC 
GATGACGATC CAGACATCTA TCCCGATACT TGTGATTGTG ATTTCGTAGC TCGCCAACTA 
ATGGACACGA ATTGGATTGT GACAAATCTA TCGACGCCTG CCAATCTCTT TCATGCTTTG 
CGCCGTCAAG TGGCCTTAGG TTTTCGTAAA CCGCTAATTA ATTTTTCACC AAAATCTGTT 
TTGCGTCACC CATTGGCCCG GAGTCCATTC CGTGATTTTA CCGAATGCAG TGAATTTATG 
CGAATAATAC CCGAAACCGG CAAAGCAGGC TGTAAACCTG ATTGTGTGGA AAAGGTTGTC 
TTTTGTTCAG GAAAGGTCTA CTACGATTTG GTCAAGGAGC GTGAGGAACA CGAGCAAGAC 
GAAACTGTGG CCATTATTCG CGTAGAACAG GTCTGTCCGT TTCCATATGA TTTGATAACC 
AAACAAATTG AACTATATAA GAATGCCGAA TTGATTTGGT GTCAGGAAGA GCATAAAAAT 
CAAGGATCTT GGTCGTATGT CCAGCAACGT TTCGATACCA CGCTAATCAA AATGGAAGAC 
GAAACCCGGT GTATATCCTA TCATGGACGT CCGCCAAATT CGGCGCCATC TACTGGCAAT 
AAGGTTCGGC ATTATACTGA ATACAAGAAT TTCATGACCA GCATTTTTGG TGAACTCACC 
GAGGAAAATA AACAGCGTCT TGAGCGGATT GTCAAAGAAC AGCAAGCGCA GGCTAAGCAG 
GCCAGTAGTA GCACTAAAGC AAAGACTGTG GGCCAAGGCG AAGACAAAAC GAAAGCCAAA 
TCCAGTAAAG ACGACAAGAA GGACGATAAG AAAGACGACA AGAAGGACGA TAAGAAAGAC 
GATAAGAAGG CTAAACCTGG AGCAACAACC GCTCCTCCCA CTTCTCCTCC AGCATCACCA 
CCACCGAAAC AAGATAATAA ATCTCCAACA ACGTCGGCGC CCAAAGGTGA CATGAATCCA 
GCTGATAAAA CTAAAGGCAA GATTGACACC AAACAAGAAA ATTCTAAAAA AGCCACTACA 
GACGAAACCA ATAAAAATGA CGAGAACCTC AAGAAACCGA CAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002065860.2
CDS	4..3471
Translation	MLVVNFSKIDVAPRLLREYARYHYRKLQTSSSVCKSSQDNTKDTDSFANGSSAAYIEGLYTKWSKNPRSVDSSWDAYFSGKPRSISTTPSLYKKSEVSADSSATKTTKAAAGAPPAADWKYIDDHLTVQAIIRAYQVRGHLAADLDPLGIVGPPGITSVDGKKRNAAQAVLRQHYTYVFNDLNATFKLPSSTMIGGDEQFLPLKEILDRLERVYCGHIGVEYMQITSLTKTNWIRERFEKPGAIMFNPDEKKLILERLTRSTGFENFLAKKFTSEKRFGLEGCDIMIPIMKEIIDRSTKLGVESIFIGMAHRGRLNVLANICRKPIPDILSQFHGLKATDSGSGDVKYHLGLFTERLNRVTNKNVRITVVANPSHLEYVNPVVLGKARAEMYQRGDSNGSKVLSIIIHGDASFCGQGVVYESMHLSDLPCYTTFGTIHIVANNQVGFTTDPRFSRSSRYCTDVARVVNAPIFHVNADDPEACIHCARVSTDYRAEFHKDVVIDIVGYRRNGHNEADEPMFTQPLMYQRIRKLQPVMQKYAEKLIKEGVVQEKEYKDMIAKYEKICDDAFIESKKVKTIKYSSWIDSPWTGFFEGRDRLKVCPTGVSLETLNNIGEIFSNPPPAEHKFEVHKGVLRILALRKQMVKSQMADWSLGEAFAIGTLLKDGIHVRLSGQDVERGTFSHRHHVFHHQSQDKGRYNSIEHLYPDQSPYVVCNSSLSECAIMGFEHGYSMAHPNTLVIWEAQFGDFANTAQTIIDSFIASGETKWVRQSGLVLLLPHSMEGMGPEHSSGRIERFLQLSDDDPDIYPDTCDCDFVARQLMDTNWIVTNLSTPANLFHALRRQVALGFRKPLINFSPKSVLRHPLARSPFRDFTECSEFMRIIPETGKAGCKPDCVEKVVFCSGKVYYDLVKEREEHEQDETVAIIRVEQVCPFPYDLITKQIELYKNAELIWCQEEHKNQGSWSYVQQRFDTTLIKMEDETRCISYHGRPPNSAPSTGNKVRHYTEYKNFMTSIFGELTEENKQRLERIVKEQQAQAKQASSSTKAKTVGQGEDKTKAKSSKDDKKDDKKDDKKDDKKDDKKAKPGATTAPPTSPPASPPPKQDNKSPTTSAPKGDMNPADKTKGKIDTKQENSKKATTDETNKNDENLKKPTK

Target ORF information:

RefSeq Version	XM_002065824.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK20459), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002065824.2

ORF Insert Sequence:

ATGTTGGTGG TGAATTTTTC GAAAATTGAT GTTGCTCCCA GGCTTTTAAG GGAGTATGCA 
CGTTACCATT ATCGGAAACT GCAAACGTCC AGTTCAGTTT GCAAGAGTAG CCAGGATAAT 
ACCAAGGACA CGGATAGTTT TGCGAATGGC AGCAGTGCCG CCTATATAGA AGGTCTATAT 
ACCAAATGGT CGAAAAACCC CAGATCAGTG GATTCGTCAT GGGATGCCTA TTTCAGTGGC 
AAGCCACGTA GTATATCCAC TACACCGTCA CTGTACAAGA AGTCCGAAGT GAGTGCAGAT 
TCGTCGGCAA CTAAAACAAC AAAAGCAGCA GCAGGAGCAC CACCGGCAGC TGACTGGAAA 
TACATAGATG ATCATCTCAC AGTCCAGGCT ATTATCCGAG CGTATCAGGT GCGAGGACAT 
TTAGCTGCCG ATCTGGATCC TTTGGGTATT GTTGGACCAC CAGGCATTAC CTCGGTAGAC 
GGCAAGAAAC GAAATGCCGC TCAAGCGGTA CTGCGCCAGC ATTATACCTA TGTATTTAAT 
GATCTCAATG CCACATTTAA ATTACCATCT TCCACCATGA TCGGCGGTGA TGAGCAGTTT 
CTGCCTTTGA AGGAAATTTT AGATCGTCTG GAGCGAGTGT ATTGTGGTCA CATTGGAGTG 
GAGTACATGC AAATTACGTC TTTGACAAAA ACCAACTGGA TACGCGAAAG ATTTGAGAAG 
CCTGGTGCCA TAATGTTTAA TCCTGATGAA AAGAAGTTAA TTCTAGAGCG CCTCACGCGT 
TCCACGGGAT TCGAAAACTT TTTGGCCAAG AAGTTTACGT CGGAGAAACG TTTCGGTTTA 
GAGGGTTGCG ACATAATGAT ACCCATCATG AAGGAGATCA TCGATAGATC GACCAAATTA 
GGAGTAGAGT CCATATTTAT AGGCATGGCG CATCGCGGCC GTCTTAATGT TCTGGCCAAT 
ATTTGTCGTA AGCCTATTCC TGATATTTTG TCACAATTCC ATGGCCTTAA GGCCACCGAT 
TCGGGATCTG GCGATGTGAA ATACCATCTT GGTCTATTTA CGGAACGTCT TAATCGAGTG 
ACAAATAAGA ATGTGCGCAT AACTGTGGTC GCCAATCCTT CACATTTGGA GTATGTGAAT 
CCGGTGGTGT TGGGCAAGGC ACGTGCTGAA ATGTATCAAC GTGGTGATAG CAATGGTAGT 
AAGGTTCTCT CCATTATCAT ACACGGCGAC GCTTCCTTCT GCGGCCAGGG TGTGGTGTAC 
GAGTCAATGC ATCTATCGGA TTTGCCATGC TACACAACTT TTGGCACCAT TCATATAGTG 
GCAAACAACC AGGTGGGTTT CACAACAGAT CCACGTTTCT CGCGCTCCTC ACGTTACTGC 
ACGGATGTCG CTCGTGTGGT CAATGCCCCA ATCTTTCATG TGAACGCCGA TGATCCTGAG 
GCATGCATAC ACTGTGCTCG CGTCTCTACC GACTATCGGG CCGAATTCCA CAAAGATGTC 
GTCATTGACA TTGTGGGCTA TCGTCGTAAT GGTCATAATG AAGCTGATGA ACCCATGTTC 
ACTCAACCCC TCATGTATCA GCGCATAAGA AAGCTTCAAC CAGTTATGCA AAAATATGCC 
GAGAAGCTAA TCAAAGAGGG GGTCGTTCAG GAGAAAGAAT ATAAGGACAT GATCGCAAAA 
TATGAGAAAA TATGCGACGA CGCTTTCATC GAGTCTAAGA AAGTGAAAAC CATCAAGTAT 
TCTTCGTGGA TCGATTCGCC GTGGACGGGC TTTTTTGAAG GGCGAGATCG TTTGAAAGTT 
TGTCCCACTG GAGTTAGCTT GGAAACCCTT AATAATATTG GTGAGATCTT CTCGAATCCG 
CCGCCTGCCG AGCACAAGTT TGAGGTGCAC AAGGGAGTCC TTCGTATATT GGCCTTGCGC 
AAGCAAATGG TAAAATCGCA AATGGCTGAC TGGTCTCTGG GTGAGGCTTT TGCCATTGGC 
ACTTTACTGA AGGATGGCAT TCATGTGCGA CTATCTGGCC AGGATGTGGA GCGGGGAACC 
TTCTCCCATC GACATCATGT CTTCCATCAT CAAAGTCAGG ACAAGGGTCG ATATAATTCC 
ATTGAGCATC TATATCCCGA TCAGTCACCT TATGTTGTTT GCAATAGCTC CTTATCGGAG 
TGTGCCATAA TGGGCTTTGA GCATGGCTAC TCGATGGCCC ATCCCAATAC ACTGGTCATA 
TGGGAGGCGC AGTTTGGTGA TTTTGCGAAC ACAGCCCAAA CAATTATCGA TTCCTTTATA 
GCCAGTGGTG AAACGAAATG GGTTCGCCAA TCTGGGCTTG TTCTTCTGCT GCCGCATTCC 
ATGGAGGGTA TGGGGCCAGA GCATTCCTCC GGTCGCATTG AACGCTTTTT GCAGCTAAGC 
GATGACGATC CAGACATCTA TCCCGATACT TGTGATTGTG ATTTCGTAGC TCGCCAACTA 
ATGGACACGA ATTGGATTGT GACAAATCTA TCGACGCCTG CCAATCTCTT TCATGCTTTG 
CGCCGTCAAG TGGCCTTAGG TTTTCGTAAA CCGCTAATTA ATTTTTCACC AAAATCTGTT 
TTGCGTCACC CATTGGCCCG GAGTCCATTC CGTGATTTTA CCGAATGCAG TGAATTTATG 
CGAATAATAC CCGAAACCGG CAAAGCAGGC TGTAAACCTG ATTGTGTGGA AAAGGTTGTC 
TTTTGTTCAG GAAAGGTCTA CTACGATTTG GTCAAGGAGC GTGAGGAACA CGAGCAAGAC 
GAAACTGTGG CCATTATTCG CGTAGAACAG GTCTGTCCGT TTCCATATGA TTTGATAACC 
AAACAAATTG AACTATATAA GAATGCCGAA TTGATTTGGT GTCAGGAAGA GCATAAAAAT 
CAAGGATCTT GGTCGTATGT CCAGCAACGT TTCGATACCA CGCTAATCAA AATGGAAGAC 
GAAACCCGGT GTATATCCTA TCATGGACGT CCGCCAAATT CGGCGCCATC TACTGGCAAT 
AAGGTTCGGC ATTATACTGA ATACAAGAAT TTCATGACCA GCATTTTTGG TGAACTCACC 
GAGGAAAATA AACAGCGTCT TGAGCGGATT GTCAAAGAAC AGCAAGCGCA GGCTAAGCAG 
GCCAGTAGTA GCACTAAAGC AAAGACTGTG GGCCAAGGCG AAGACAAAAC GAAAGCCAAA 
TCCAGTAAAG ACGACAAGAA GGACGATAAG AAAGACGACA AGAAGGACGA TAAGAAAGAC 
GATAAGAAGG CTAAACCTGG AGCAACAACC GCTCCTCCCA CTTCTCCTCC AGCATCACCA 
CCACCGAAAC AAGATAATAA ATCTCCAACA ACGTCGGCGC CCAAAGGTGA CATGAATCCA 
GCTGATAAAA CTAAAGGCAA GATTGACACC AAACAAGAAA ATTCTAAAAA AGCCACTACA 
GACGAAACCA ATAAAAATGA CGAGAACCTC AAGAAACCGA CAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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DWIL\GK20459 cDNA ORF clone, Drosophila willistoni

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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