DWIL\GK20677 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DWIL\GK20677
Entrez Gene ID	6638412
Full Name	nephrin
Synonyms	GK20677,GK20677-PB
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_023177252.1	XP_023033020.1	nephrin
XM_002061471.2	XP_002061507.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DWIL\GK20677 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWIL\GK20677 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODf21810	XM_002061471.2 Latest version!	Drosophila willistoni uncharacterized protein (Dwil\GK20677), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODf00877	XM_023177252.1 Latest version!	Drosophila willistoni nephrin (LOC6638412), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWIL\GK20677 ( XM_002061471.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002061507.2 Drosophila willistoni uncharacterized protein (Dwil\GK20677), partial mRNA.

Price & Availability↑

CloneID	ODf21810
Clone ID Related Accession (Same CDS sequence)	XM_002061471.2
Accession Version	XM_002061471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032467). On Dec 21, 2015 this sequence version replaced XM_002061471.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGCCTAAAA ACGATAGAGA AACAGCAGGA GGCGAGGGAC CAAGGAGCAG GGAGCTGGGA 
AAGGGACAAG CAGACATTCT TGAGAGCAAC TCCAATTTCA GTGTGTTCTT TGCCCTCTAT 
CTATGGCCAT GGTCATTGCC CTATGCTCGA GCCCAGCAAC AGAAATTCCG CACAACGCCT 
CATGATCTTC AAGTTCTGGA GGGTGCCGAG GCCATGATGC GCTGTGAAGT CACAAATTTG 
GCAGGCGCTG TTCAATGGAC GAAAGATGGC TTCGCCTTGG GCTTTGAGGC AGTCATACCG 
GGATTTCCAC GCTACTCCGT GCTCGGAGAT CGCAAACAGG GTGTCTATAA TTTGCGCATA 
TCGAATGCCT CAATCAACGA TGATGCCGAA TATCAATGTC AAGTCGGTCC GGCGAGATTA 
CAATCGGCCA TCAGAGCGAA TGCCAAACTC ACCGTCATAT CTCCACCGTC ATCGATCGAA 
ATCAAGGGTT ATAGTCACAA CTCGAAGGTT GAAGTGCGTG AGAATCAGGA CTTGCAACTC 
AAATGCATTG TGGCCAATGC AAAGCCAGCG GCTCAAATAG TCTGGTATCG CGGCAATACT 
GAATACAAAC CAGAAAAACG GGAAGATAAG GTTGAAGAAT CGACGCCAAA ACGATTTACC 
ACCACATCCA TTTTGAAGTT GAAACCAGGT CCTGATGACG ATTATACGGA ATATACGTGC 
CAGGCCAAAC ATAAGGCTTT ATCGCCGGAT ATGCCAATGC GTGCAACGGT TCAACTGTCC 
GTGTTGTATC CACCTGGTCC ACCCTATATT GAAGGTTATT CAGCTGGTGA AACATTGCGC 
CGTGGCCAAA CAGTGGAGCT TATGTGCCGC AGTCGTGGTG GCAATCCTCC AGCTCAACTT 
ATTTGGTATA AAAATGGCTC ACAAATACGC ATGGCCTATA GAACCTCTGG ACGTTTGTCG 
GAGAATATTT ATACTTTTAC TGCCGAAGCG GGGGATAATA AGGCTCGTTT TCGCTGCGAG 
GCCAGCAATG TGATGTCAAA GGATCCCCTT AAAGCTGAAG TGGAATTATC TGTTCTATTT 
GCACCTTCAC ACGTCACCGT TACGGGTCCC ACGGAGGCTC GTGTGGGGGA TGTTGTGCCA 
CTTACGTGCA CCACAGCACC ATCGAATCCA CCCGCTGAGA TCAAATGGAT GGTGGGCGGA 
CGTCAGATAC GCAATGCCAC ATCAAAGACC ATTGTCAGTC CCGAAGGCGG CTGGACTACT 
TCATCCAATA TTAGCACTAT TGTGGAGCCA AATAAACGTT CCTTGGTAAT CATTTGCCAT 
GGCCTTAATA TGCAGCTAAC AGAGAATGTT GTCTCCACCC ATACGATCAA TGTCCTGTAT 
CCACCCGCAC CGCCATTAAT TTCTGGCTAT ATAGAAGGAC AAATTATACC AGCTGGTTCA 
GTGCAAAAAC TGCTTTGCGT TTCATCAGGC GGCAATCCGT TGGCAACGTT AACGTGGTAT 
AAAAACGACA AAAGGATCAA CTCGGTTTTA CGCGCCGCGG ACAAATCGGT TTCAGCGGAA 
ATAACCATCT TGGCCAACGT TACCGACAAC CAGGCGCAAT ATCGTTGCGA GGCCTCGAAT 
AGCGCCACGG AGATACCGCT CTTCCAGTCC ACAACGCTCA GTGTGCATTT TGCCCCGGAG 
ACGGTAAAAA TACGCATTGA ACCGGAAGAG CTGCGACCTG GCATGGAGGC CTCCATCATT 
TGCGACTCGA GCTCAAGCAA TCCGCCGGCC AAGCTGTCCT GGTGGAAGGA CGGCATCTCC 
ATTGAAGGCA TTAATAACAC GTCCAAGCCC GGACTCTGGG GCGGCACAGT CTCTACGCTC 
GAATTCAGGG TAAACATCTC ACAGGAAATG AACGGCATAG TCTACACTTG CCAAAGCGCC 
AACGAGGCAC TACAACGTAG TGTCCATGAG GCCATAAGCC TGGATGTTCT ATATCGTCCC 
AAGTTTGTGC CGCCGCCATC CTCAACGGCT GTCGGTGTGG AAGGTGAATC CCTGCAGGTG 
TCGCTCCAGG CGAATGGTAA TCCTGCAGCC ATTGCCTATA CATGGACAAA GGATGGCATG 
AGTATACCCC AAAGCGATGG CGTTGAGCAT CGTATATTTG CCGAAGCGGG CACTTTGAAT 
TTTACCCGCC TGCATCGTGA CGATGCGGGG GTCTATTCCT GTAGTGCTGG CAACTCGCAA 
GGCAAGGCTG CACTCAATAT CACAGTGGTA GTGGAATATG GCACTACCAT CAAGTCTATA 
TCAGAGAACA TCATTGTGAA TCCTGGTGAG GAGGCAATGC TTTCGTGTAC TGTTGAGGGT 
AAACCTCTAA CAGAGGAGCA TGTCAAGTGG GAACGAGTTG GCTACGATAT GGCCGCCAAG 
ACAACAACAA CGTTTGCCAA TGGCACATCC TATCTGCACA TTAAGGATGC TCAACGCGAA 
GATGTTGGTA ACTTTCGTTG TGTGGCCGAC AATCGTGTGG CCAATCCCAC CAATCGAGAT 
GTCCTGCTGA TAGTCAAGTT TGCTCCGGAA ATAGCCAAGT CACCGACACT GCTGAGAGCT 
GCCAGCGGTA CAGGTGAGCG TGGTCGTTTA CCGTGTCGTG CGCAGGCATC CCCGAAGCCA 
GAATTCATTT GGCGCCAGGA TAAAAAAGAT TTGCCCATTA ATCGTACATA TAAATATGAA 
GTGGAAGAGC GTAAAATCGA CTCATTGACC TATGAATCTA CGCTAATTGT ACAAAAGGTG 
GCTCCCGCCG ACTACGGGGC CTACGAATGT GTGGCCCGCA ATGAACTAGG CGAAACGATA 
GAGACCGTCC GCTTGGATAT CACATCACCA CCAGATCCAC CACTTAGTCT CAATATCTTG 
AATGTTACCC ACGATACAGT GACTGTTGCC TGGACGCCTG GTTTTGATGG CGGCCTAAAG 
GCCTCGTATC GTGTGCGCTA TCGGATGGCC GACCGTGAGC AATACAAGTA CATTGATGGA 
TTGCCCAATA GCCATAAGCT AACGATTGCT GGATTGCGTA TGAACACACT TTACTTGTTC 
TCCGTCATGT CAAAGAATGA GTTGGGTCAG AGTGTCTATT TGCCGGATTT AACGCGTGCC 
CAGACCAAAG GACTTGAAAT GCTGCCAGCG GAGTTAACTG AGGACTCTAG CAATACACCG 
AATCTGGTCA TAATTGGCAT ATCGCTGGCG GCATTTGGCT TTCTCCTGGT CAATGCGGCG 
CTTGTTGCCT GGTTCTTTGT GCATCAGCGG CGCAAGAAAG TGGCGGAAAC TACAAATCAA 
CCAGGCAAAA CGGCAACCAT CGAAATGTAT GCCCCCAGTT CGTACAATGA CACCGTTACC 
GGCGAGACAC TGTCCAGTGT GTCGGAAAAG AGCGAATCCT ACTCGAATGA GGGCAGCACT 
CAGCCGGAAT ATATTGACGA GGCTCGTAAG AAGGCTGCCT CAACATATTT GGTTGAAGGG 
ACAGCAGATA TGCCACCGCC CAGATATCAA AAGGATGGCA CTTTGCCAGT ATTATATCCG 
AATAATATGG TAAATGCCTG CACCCTGCCA CATCCGAGGC ATAACAATGC AATTGGAGCA 
ACAGCAGCTG GTAGCAATAA TGCTGCTGCA GCCATCCATA TGACGCGAGA CGATCAGATG 
CTCATCACCA AGGGCGTTTA TATTCCGTCC CCATCGCCAG CGCCACCGCC AGATGGTTCC 
TATTATAATA TGAATAGCGA TAGATATCTA TCCTATCCGC CAATGGAATA TACGGCTGCC 
TTGGACTTTA GTGCCCCACC GTTGCCCATG ACGCATCTGC AACCCATTAC GGTCACCTCG 
CAAGCCATGT TGACCTCAAA TGGCGGTGCC ACCCTGATAG CCAATGGCAA TGCAGCCAAT 
GCAGGTGTTG GTGGTGGTGG TTCAGGCACT CTGAGACGTG GAATTCTGCG TGGAGTTGTG 
GGTGTACCAC CACCGGATGT CACCCATCAC ACCTCACCGG GCAGTCCCAT GCAAATATTG 
CATGATCTGC ATCATCCCAT CAATTTGAGT GCCACAACGT TAACAACTAG CACCACACTA 
AATGGCAGCA TGACATCAGC ATCAACGCAT GGGACACTGC CACGTCAACC GCAGAGTATC 
CTAAAGGACC CAAATCGAAA TAAGCAGCAA CAGCAGCAAC AACATCAGCA GCAGCAGCAG 
CTTCTCAATG CCAGCCTAGT GGGTGTTGGT GTTCCTGTCT CAGGTCCGGT GGGGAGCCTG 
CAGATACTGA ATCTGCCCGC TGTGGGTCCT GGCCTGGGTA ACAATCTATT AATGACTACC 
AGCGCCACTT ATGATCCAAG TGGCGTTGGA CCTATGAGTA GTTTTAATGC TGCCAATGTG 
GCAGCTGTCT CACAGGGTCT TTCCTGTCCG GTGGCCTATA CAGATGCGGA TGGACATCTT 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002061507.2
CDS	1..4446
Translation	MPKNDRETAGGEGPRSRELGKGQADILESNSNFSVFFALYLWPWSLPYARAQQQKFRTTPHDLQVLEGAEAMMRCEVTNLAGAVQWTKDGFALGFEAVIPGFPRYSVLGDRKQGVYNLRISNASINDDAEYQCQVGPARLQSAIRANAKLTVISPPSSIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNTEYKPEKREDKVEESTPKRFTTTSILKLKPGPDDDYTEYTCQAKHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSKDPLKAEVELSVLFAPSHVTVTGPTEARVGDVVPLTCTTAPSNPPAEIKWMVGGRQIRNATSKTIVSPEGGWTTSSNISTIVEPNKRSLVIICHGLNMQLTENVVSTHTINVLYPPAPPLISGYIEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVLRAADKSVSAEITILANVTDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEASIICDSSSSNPPAKLSWWKDGISIEGINNTSKPGLWGGTVSTLEFRVNISQEMNGIVYTCQSANEALQRSVHEAISLDVLYRPKFVPPPSSTAVGVEGESLQVSLQANGNPAAIAYTWTKDGMSIPQSDGVEHRIFAEAGTLNFTRLHRDDAGVYSCSAGNSQGKAALNITVVVEYGTTIKSISENIIVNPGEEAMLSCTVEGKPLTEEHVKWERVGYDMAAKTTTTFANGTSYLHIKDAQREDVGNFRCVADNRVANPTNRDVLLIVKFAPEIAKSPTLLRAASGTGERGRLPCRAQASPKPEFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVQKVAPADYGAYECVARNELGETIETVRLDITSPPDPPLSLNILNVTHDTVTVAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNSHKLTIAGLRMNTLYLFSVMSKNELGQSVYLPDLTRAQTKGLEMLPAELTEDSSNTPNLVIIGISLAAFGFLLVNAALVAWFFVHQRRKKVAETTNQPGKTATIEMYAPSSYNDTVTGETLSSVSEKSESYSNEGSTQPEYIDEARKKAASTYLVEGTADMPPPRYQKDGTLPVLYPNNMVNACTLPHPRHNNAIGATAAGSNNAAAAIHMTRDDQMLITKGVYIPSPSPAPPPDGSYYNMNSDRYLSYPPMEYTAALDFSAPPLPMTHLQPITVTSQAMLTSNGGATLIANGNAANAGVGGGGSGTLRRGILRGVVGVPPPDVTHHTSPGSPMQILHDLHHPINLSATTLTTSTTLNGSMTSASTHGTLPRQPQSILKDPNRNKQQQQQQHQQQQQLLNASLVGVGVPVSGPVGSLQILNLPAVGPGLGNNLLMTTSATYDPSGVGPMSSFNAANVAAVSQGLSCPVAYTDADGHLV

Target ORF information:

RefSeq Version	XM_002061471.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK20677), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002061471.2

ORF Insert Sequence:

ATGCCTAAAA ACGATAGAGA AACAGCAGGA GGCGAGGGAC CAAGGAGCAG GGAGCTGGGA 
AAGGGACAAG CAGACATTCT TGAGAGCAAC TCCAATTTCA GTGTGTTCTT TGCCCTCTAT 
CTATGGCCAT GGTCATTGCC CTATGCTCGA GCCCAGCAAC AGAAATTCCG CACAACGCCT 
CATGATCTTC AAGTTCTGGA GGGTGCCGAG GCCATGATGC GCTGTGAAGT CACAAATTTG 
GCAGGCGCTG TTCAATGGAC GAAAGATGGC TTCGCCTTGG GCTTTGAGGC AGTCATACCG 
GGATTTCCAC GCTACTCCGT GCTCGGAGAT CGCAAACAGG GTGTCTATAA TTTGCGCATA 
TCGAATGCCT CAATCAACGA TGATGCCGAA TATCAATGTC AAGTCGGTCC GGCGAGATTA 
CAATCGGCCA TCAGAGCGAA TGCCAAACTC ACCGTCATAT CTCCACCGTC ATCGATCGAA 
ATCAAGGGTT ATAGTCACAA CTCGAAGGTT GAAGTGCGTG AGAATCAGGA CTTGCAACTC 
AAATGCATTG TGGCCAATGC AAAGCCAGCG GCTCAAATAG TCTGGTATCG CGGCAATACT 
GAATACAAAC CAGAAAAACG GGAAGATAAG GTTGAAGAAT CGACGCCAAA ACGATTTACC 
ACCACATCCA TTTTGAAGTT GAAACCAGGT CCTGATGACG ATTATACGGA ATATACGTGC 
CAGGCCAAAC ATAAGGCTTT ATCGCCGGAT ATGCCAATGC GTGCAACGGT TCAACTGTCC 
GTGTTGTATC CACCTGGTCC ACCCTATATT GAAGGTTATT CAGCTGGTGA AACATTGCGC 
CGTGGCCAAA CAGTGGAGCT TATGTGCCGC AGTCGTGGTG GCAATCCTCC AGCTCAACTT 
ATTTGGTATA AAAATGGCTC ACAAATACGC ATGGCCTATA GAACCTCTGG ACGTTTGTCG 
GAGAATATTT ATACTTTTAC TGCCGAAGCG GGGGATAATA AGGCTCGTTT TCGCTGCGAG 
GCCAGCAATG TGATGTCAAA GGATCCCCTT AAAGCTGAAG TGGAATTATC TGTTCTATTT 
GCACCTTCAC ACGTCACCGT TACGGGTCCC ACGGAGGCTC GTGTGGGGGA TGTTGTGCCA 
CTTACGTGCA CCACAGCACC ATCGAATCCA CCCGCTGAGA TCAAATGGAT GGTGGGCGGA 
CGTCAGATAC GCAATGCCAC ATCAAAGACC ATTGTCAGTC CCGAAGGCGG CTGGACTACT 
TCATCCAATA TTAGCACTAT TGTGGAGCCA AATAAACGTT CCTTGGTAAT CATTTGCCAT 
GGCCTTAATA TGCAGCTAAC AGAGAATGTT GTCTCCACCC ATACGATCAA TGTCCTGTAT 
CCACCCGCAC CGCCATTAAT TTCTGGCTAT ATAGAAGGAC AAATTATACC AGCTGGTTCA 
GTGCAAAAAC TGCTTTGCGT TTCATCAGGC GGCAATCCGT TGGCAACGTT AACGTGGTAT 
AAAAACGACA AAAGGATCAA CTCGGTTTTA CGCGCCGCGG ACAAATCGGT TTCAGCGGAA 
ATAACCATCT TGGCCAACGT TACCGACAAC CAGGCGCAAT ATCGTTGCGA GGCCTCGAAT 
AGCGCCACGG AGATACCGCT CTTCCAGTCC ACAACGCTCA GTGTGCATTT TGCCCCGGAG 
ACGGTAAAAA TACGCATTGA ACCGGAAGAG CTGCGACCTG GCATGGAGGC CTCCATCATT 
TGCGACTCGA GCTCAAGCAA TCCGCCGGCC AAGCTGTCCT GGTGGAAGGA CGGCATCTCC 
ATTGAAGGCA TTAATAACAC GTCCAAGCCC GGACTCTGGG GCGGCACAGT CTCTACGCTC 
GAATTCAGGG TAAACATCTC ACAGGAAATG AACGGCATAG TCTACACTTG CCAAAGCGCC 
AACGAGGCAC TACAACGTAG TGTCCATGAG GCCATAAGCC TGGATGTTCT ATATCGTCCC 
AAGTTTGTGC CGCCGCCATC CTCAACGGCT GTCGGTGTGG AAGGTGAATC CCTGCAGGTG 
TCGCTCCAGG CGAATGGTAA TCCTGCAGCC ATTGCCTATA CATGGACAAA GGATGGCATG 
AGTATACCCC AAAGCGATGG CGTTGAGCAT CGTATATTTG CCGAAGCGGG CACTTTGAAT 
TTTACCCGCC TGCATCGTGA CGATGCGGGG GTCTATTCCT GTAGTGCTGG CAACTCGCAA 
GGCAAGGCTG CACTCAATAT CACAGTGGTA GTGGAATATG GCACTACCAT CAAGTCTATA 
TCAGAGAACA TCATTGTGAA TCCTGGTGAG GAGGCAATGC TTTCGTGTAC TGTTGAGGGT 
AAACCTCTAA CAGAGGAGCA TGTCAAGTGG GAACGAGTTG GCTACGATAT GGCCGCCAAG 
ACAACAACAA CGTTTGCCAA TGGCACATCC TATCTGCACA TTAAGGATGC TCAACGCGAA 
GATGTTGGTA ACTTTCGTTG TGTGGCCGAC AATCGTGTGG CCAATCCCAC CAATCGAGAT 
GTCCTGCTGA TAGTCAAGTT TGCTCCGGAA ATAGCCAAGT CACCGACACT GCTGAGAGCT 
GCCAGCGGTA CAGGTGAGCG TGGTCGTTTA CCGTGTCGTG CGCAGGCATC CCCGAAGCCA 
GAATTCATTT GGCGCCAGGA TAAAAAAGAT TTGCCCATTA ATCGTACATA TAAATATGAA 
GTGGAAGAGC GTAAAATCGA CTCATTGACC TATGAATCTA CGCTAATTGT ACAAAAGGTG 
GCTCCCGCCG ACTACGGGGC CTACGAATGT GTGGCCCGCA ATGAACTAGG CGAAACGATA 
GAGACCGTCC GCTTGGATAT CACATCACCA CCAGATCCAC CACTTAGTCT CAATATCTTG 
AATGTTACCC ACGATACAGT GACTGTTGCC TGGACGCCTG GTTTTGATGG CGGCCTAAAG 
GCCTCGTATC GTGTGCGCTA TCGGATGGCC GACCGTGAGC AATACAAGTA CATTGATGGA 
TTGCCCAATA GCCATAAGCT AACGATTGCT GGATTGCGTA TGAACACACT TTACTTGTTC 
TCCGTCATGT CAAAGAATGA GTTGGGTCAG AGTGTCTATT TGCCGGATTT AACGCGTGCC 
CAGACCAAAG GACTTGAAAT GCTGCCAGCG GAGTTAACTG AGGACTCTAG CAATACACCG 
AATCTGGTCA TAATTGGCAT ATCGCTGGCG GCATTTGGCT TTCTCCTGGT CAATGCGGCG 
CTTGTTGCCT GGTTCTTTGT GCATCAGCGG CGCAAGAAAG TGGCGGAAAC TACAAATCAA 
CCAGGCAAAA CGGCAACCAT CGAAATGTAT GCCCCCAGTT CGTACAATGA CACCGTTACC 
GGCGAGACAC TGTCCAGTGT GTCGGAAAAG AGCGAATCCT ACTCGAATGA GGGCAGCACT 
CAGCCGGAAT ATATTGACGA GGCTCGTAAG AAGGCTGCCT CAACATATTT GGTTGAAGGG 
ACAGCAGATA TGCCACCGCC CAGATATCAA AAGGATGGCA CTTTGCCAGT ATTATATCCG 
AATAATATGG TAAATGCCTG CACCCTGCCA CATCCGAGGC ATAACAATGC AATTGGAGCA 
ACAGCAGCTG GTAGCAATAA TGCTGCTGCA GCCATCCATA TGACGCGAGA CGATCAGATG 
CTCATCACCA AGGGCGTTTA TATTCCGTCC CCATCGCCAG CGCCACCGCC AGATGGTTCC 
TATTATAATA TGAATAGCGA TAGATATCTA TCCTATCCGC CAATGGAATA TACGGCTGCC 
TTGGACTTTA GTGCCCCACC GTTGCCCATG ACGCATCTGC AACCCATTAC GGTCACCTCG 
CAAGCCATGT TGACCTCAAA TGGCGGTGCC ACCCTGATAG CCAATGGCAA TGCAGCCAAT 
GCAGGTGTTG GTGGTGGTGG TTCAGGCACT CTGAGACGTG GAATTCTGCG TGGAGTTGTG 
GGTGTACCAC CACCGGATGT CACCCATCAC ACCTCACCGG GCAGTCCCAT GCAAATATTG 
CATGATCTGC ATCATCCCAT CAATTTGAGT GCCACAACGT TAACAACTAG CACCACACTA 
AATGGCAGCA TGACATCAGC ATCAACGCAT GGGACACTGC CACGTCAACC GCAGAGTATC 
CTAAAGGACC CAAATCGAAA TAAGCAGCAA CAGCAGCAAC AACATCAGCA GCAGCAGCAG 
CTTCTCAATG CCAGCCTAGT GGGTGTTGGT GTTCCTGTCT CAGGTCCGGT GGGGAGCCTG 
CAGATACTGA ATCTGCCCGC TGTGGGTCCT GGCCTGGGTA ACAATCTATT AATGACTACC 
AGCGCCACTT ATGATCCAAG TGGCGTTGGA CCTATGAGTA GTTTTAATGC TGCCAATGTG 
GCAGCTGTCT CACAGGGTCT TTCCTGTCCG GTGGCCTATA CAGATGCGGA TGGACATCTT 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWIL\GK20677 ( XM_023177252.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023033020.1 Drosophila willistoni nephrin (LOC6638412), mRNA.

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CloneID	ODf00877
Clone ID Related Accession (Same CDS sequence)	XM_023177252.1
Accession Version	XM_023177252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032467.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTGA AAACAGAAAT GACTGCAGAG GAACAACAAC AGCAGCAGCA GCAGCAACAT 
ACAACAAAAA GACGTCGACG GCGTTGCAAA GAACAGTGGA TTAAAAATCA TTTACAATTG 
CTATGCATAG TTGCCCTCTA TCTATGGCCA TGGTCATTGC CCTATGCTCG AGCCCAGCAA 
CAGAAATTCC GCACAACGCC TCATGATCTT CAAGTTCTGG AGGGTGCCGA GGCCATGATG 
CGCTGTGAAG TCACAAATTT GGCAGGCGCT GTTCAATGGA CGAAAGATGG CTTCGCCTTG 
GGCTTTGAGG CAGTCATACC GGGATTTCCA CGCTACTCCG TGCTCGGAGA TCGCAAACAG 
GGTGTCTATA ATTTGCGCAT ATCGAATGCC TCAATCAACG ATGATGCCGA ATATCAATGT 
CAAGTCGGTC CGGCGAGATT ACAATCGGCC ATCAGAGCGA ATGCCAAACT CACCGTCATA 
TCTCCACCGT CATCGATCGA AATCAAGGGT TATAGTCACA ACTCGAAGGT TGAAGTGCGT 
GAGAATCAGG ACTTGCAACT CAAATGCATT GTGGCCAATG CAAAGCCAGC GGCTCAAATA 
GTCTGGTATC GCGGCAATAC TGAATACAAA CCAGAAAAAC GGGAAGATAA GGTTGAAGAA 
TCGACGCCAA AACGATTTAC CACCACATCC ATTTTGAAGT TGAAACCAGG TCCTGATGAC 
GATTATACGG AATATACGTG CCAGGCCAAA CATAAGGCTT TATCGCCGGA TATGCCAATG 
CGTGCAACGG TTCAACTGTC CGTGTTGTAT CCACCTGGTC CACCCTATAT TGAAGGTTAT 
TCAGCTGGTG AAACATTGCG CCGTGGCCAA ACAGTGGAGC TTATGTGCCG CAGTCGTGGT 
GGCAATCCTC CAGCTCAACT TATTTGGTAT AAAAATGGCT CACAAATACG CATGGCCTAT 
AGAACCTCTG GACGTTTGTC GGAGAATATT TATACTTTTA CTGCCGAAGC GGGGGATAAT 
AAGGCTCGTT TTCGCTGCGA GGCCAGCAAT GTGATGTCAA AGGATCCCCT TAAAGCTGAA 
GTGGAATTAT CTGTTCTATT TGCACCTTCA CACGTCACCG TTACGGGTCC CACGGAGGCT 
CGTGTGGGGG ATGTTGTGCC ACTTACGTGC ACCACAGCAC CATCGAATCC ACCCGCTGAG 
ATCAAATGGA TGGTGGGCGG ACGTCAGATA CGCAATGCCA CATCAAAGAC CATTGTCAGT 
CCCGAAGGCG GCTGGACTAC TTCATCCAAT ATTAGCACTA TTGTGGAGCC AAATAAACGT 
TCCTTGGTAA TCATTTGCCA TGGCCTTAAT ATGCAGCTAA CAGAGAATGT TGTCTCCACC 
CATACGATCA ATGTCCTGTA TCCACCCGCA CCGCCATTAA TTTCTGGCTA TATAGAAGGA 
CAAATTATAC CAGCTGGTTC AGTGCAAAAA CTGCTTTGCG TTTCATCAGG CGGCAATCCG 
TTGGCAACGT TAACGTGGTA TAAAAACGAC AAAAGGATCA ACTCGGTTTT ACGCGCCGCG 
GACAAATCGG TTTCAGCGGA AATAACCATC TTGGCCAACG TTACCGACAA CCAGGCGCAA 
TATCGTTGCG AGGCCTCGAA TAGCGCCACG GAGATACCGC TCTTCCAGTC CACAACGCTC 
AGTGTGCATT TTGCCCCGGA GACGGTAAAA ATACGCATTG AACCGGAAGA GCTGCGACCT 
GGCATGGAGG CCTCCATCAT TTGCGACTCG AGCTCAAGCA ATCCGCCGGC CAAGCTGTCC 
TGGTGGAAGG ACGGCATCTC CATTGAAGGC ATTAATAACA CGTCCAAGCC CGGACTCTGG 
GGCGGCACAG TCTCTACGCT CGAATTCAGG GTAAACATCT CACAGGAAAT GAACGGCATA 
GTCTACACTT GCCAAAGCGC CAACGAGGCA CTACAACGTA GTGTCCATGA GGCCATAAGC 
CTGGATGTTC TATATCGTCC CAAGTTTGTG CCGCCGCCAT CCTCAACGGC TGTCGGTGTG 
GAAGGTGAAT CCCTGCAGGT GTCGCTCCAG GCGAATGGTA ATCCTGCAGC CATTGCCTAT 
ACATGGACAA AGGATGGCAT GAGTATACCC CAAAGCGATG GCGTTGAGCA TCGTATATTT 
GCCGAAGCGG GCACTTTGAA TTTTACCCGC CTGCATCGTG ACGATGCGGG GGTCTATTCC 
TGTAGTGCTG GCAACTCGCA AGGCAAGGCT GCACTCAATA TCACAGTGGT AGTGGAATAT 
GGCACTACCA TCAAGTCTAT ATCAGAGAAC ATCATTGTGA ATCCTGGTGA GGAGGCAATG 
CTTTCGTGTA CTGTTGAGGG TAAACCTCTA ACAGAGGAGC ATGTCAAGTG GGAACGAGTT 
GGCTACGATA TGGCCGCCAA GACAACAACA ACGTTTGCCA ATGGCACATC CTATCTGCAC 
ATTAAGGATG CTCAACGCGA AGATGTTGGT AACTTTCGTT GTGTGGCCGA CAATCGTGTG 
GCCAATCCCA CCAATCGAGA TGTCCTGCTG ATAGTCAAGT TTGCTCCGGA AATAGCCAAG 
TCACCGACAC TGCTGAGAGC TGCCAGCGGT ACAGGTGAGC GTGGTCGTTT ACCGTGTCGT 
GCGCAGGCAT CCCCGAAGCC AGAATTCATT TGGCGCCAGG ATAAAAAAGA TTTGCCCATT 
AATCGTACAT ATAAATATGA AGTGGAAGAG CGTAAAATCG ACTCATTGAC CTATGAATCT 
ACGCTAATTG TACAAAAGGT GGCTCCCGCC GACTACGGGG CCTACGAATG TGTGGCCCGC 
AATGAACTAG GCGAAACGAT AGAGACCGTC CGCTTGGATA TCACATCACC ACCAGATCCA 
CCACTTAGTC TCAATATCTT GAATGTTACC CACGATACAG TGACTGTTGC CTGGACGCCT 
GGTTTTGATG GCGGCCTAAA GGCCTCGTAT CGTGTGCGCT ATCGGATGGC CGACCGTGAG 
CAATACAAGT ACATTGATGG ATTGCCCAAT AGCCATAAGC TAACGATTGC TGGATTGCGT 
ATGAACACAC TTTACTTGTT CTCCGTCATG TCAAAGAATG AGTTGGGTCA GAGTGTCTAT 
TTGCCGGATT TAACGCGTGC CCAGACCAAA GAAGCACCAC CACCGTCACA GCCAGCCTCA 
TCACTGGGCG GTGGACCACC GACCACCAGT CAGACACCAT TGGGTGGTAC ATCGGGAATG 
TTGCTGTTTG TTGGTGTTTC GGCTGGCATT GCTGTCGTTG TAGTCAACAT TCTTGTCATC 
GGCTGTTGCC TGCATAAACG CAACGAGAAG CGCCTAAAGC GAGGACTTGA AATGCTGCCA 
GCGGAGTTAA CTGAGGACTC TAGCAATACA CCGAATCTGG TCATAATTGG CATATCGCTG 
GCGGCATTTG GCTTTCTCCT GGTCAATGCG GCGCTTGTTG CCTGGTTCTT TGTGCATCAG 
CGGCGCAAGA AAGTGGCGGA AACTACAAAT CAACCAGGCA AAACGGCAAC CATCGAAATG 
TATGCCCCCA GTTCGTACAA TGACACCGTT ACCGGCGAGA CACTGTCCAG TGTGTCGGAA 
AAGAGCGAAT CCTACTCGAA TGAGGGCAGC ACTCAGCCGG AATATATTGA CGAGGCTCGT 
AAGAAGGCTG CCTCAACATA TTTGGTTGAA GGGACAGCAG ATATGCCACC GCCCAGATAT 
CAAAAGGATG GCACTTTGCC AGTATTATAT CCGAATAATA TGGTAAATGC CTGCACCCTG 
CCACATCCGA GGCATAACAA TGCAATTGGA GCAACAGCAG CTGGTAGCAA TAATGCTGCT 
GCAGCCATCC ATATGACGCG AGACGATCAG ATGCTCATCA CCAAGGGCGT TTATATTCCG 
TCCCCATCGC CAGCGCCACC GCCAGATGGT TCCTATTATA ATATGAATAG CGATAGATAT 
CTATCCTATC CGCCAATGGA ATATACGGCT GCCTTGGACT TTAGTGCCCC ACCGTTGCCC 
ATGACGCATC TGCAACCCAT TACGGTCACC TCGCAAGCCA TGTTGACCTC AAATGGCGGT 
GCCACCCTGA TAGCCAATGG CAATGCAGCC AATGCAGGTG TTGGTGGTGG TGGTTCAGGC 
ACTCTGAGAC GTGGAATTCT GCGTGGAGTT GTGGGTGTAC CACCACCGGA TGTCACCCAT 
CACACCTCAC CGGGCAGTCC CATGCAAATA TTGCATGATC TGCATCATCC CATCAATTTG 
AGTGCCACAA CGTTAACAAC TAGCACCACA CTAAATGGCA GCATGACATC AGCATCAACG 
CATGGGACAC TGCCACGTCA ACCGCAGAGT ATCCTAAAGG ACCCAAATCG AAATAAGCAG 
CAACAGCAGC AACAACATCA GCAGCAGCAG CAGCTTCTCA ATGCCAGCCT AGTGGGTGTT 
GGTGTTCCTG TCTCAGGTCC GGTGGGGAGC CTGCAGATAC TGAATCTGCC CGCTGTGGGT 
CCTGGCCTGG GTAACAATCT ATTAATGACT ACCAGCGCCA CTTATGATCC AAGTGGCGTT 
GGACCTATGA GTAGTTTTAA TGCTGCCAAT GTGGCAGCTG TCTCACAGGG TCTTTCCTGT 
CCGGTGGCCT ATACAGATGC GGATGGACAT CTTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023033020.1
CDS	164..4822
Translation	MALKTEMTAEEQQQQQQQQHTTKRRRRRCKEQWIKNHLQLLCIVALYLWPWSLPYARAQQQKFRTTPHDLQVLEGAEAMMRCEVTNLAGAVQWTKDGFALGFEAVIPGFPRYSVLGDRKQGVYNLRISNASINDDAEYQCQVGPARLQSAIRANAKLTVISPPSSIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNTEYKPEKREDKVEESTPKRFTTTSILKLKPGPDDDYTEYTCQAKHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSKDPLKAEVELSVLFAPSHVTVTGPTEARVGDVVPLTCTTAPSNPPAEIKWMVGGRQIRNATSKTIVSPEGGWTTSSNISTIVEPNKRSLVIICHGLNMQLTENVVSTHTINVLYPPAPPLISGYIEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVLRAADKSVSAEITILANVTDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEASIICDSSSSNPPAKLSWWKDGISIEGINNTSKPGLWGGTVSTLEFRVNISQEMNGIVYTCQSANEALQRSVHEAISLDVLYRPKFVPPPSSTAVGVEGESLQVSLQANGNPAAIAYTWTKDGMSIPQSDGVEHRIFAEAGTLNFTRLHRDDAGVYSCSAGNSQGKAALNITVVVEYGTTIKSISENIIVNPGEEAMLSCTVEGKPLTEEHVKWERVGYDMAAKTTTTFANGTSYLHIKDAQREDVGNFRCVADNRVANPTNRDVLLIVKFAPEIAKSPTLLRAASGTGERGRLPCRAQASPKPEFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVQKVAPADYGAYECVARNELGETIETVRLDITSPPDPPLSLNILNVTHDTVTVAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNSHKLTIAGLRMNTLYLFSVMSKNELGQSVYLPDLTRAQTKEAPPPSQPASSLGGGPPTTSQTPLGGTSGMLLFVGVSAGIAVVVVNILVIGCCLHKRNEKRLKRGLEMLPAELTEDSSNTPNLVIIGISLAAFGFLLVNAALVAWFFVHQRRKKVAETTNQPGKTATIEMYAPSSYNDTVTGETLSSVSEKSESYSNEGSTQPEYIDEARKKAASTYLVEGTADMPPPRYQKDGTLPVLYPNNMVNACTLPHPRHNNAIGATAAGSNNAAAAIHMTRDDQMLITKGVYIPSPSPAPPPDGSYYNMNSDRYLSYPPMEYTAALDFSAPPLPMTHLQPITVTSQAMLTSNGGATLIANGNAANAGVGGGGSGTLRRGILRGVVGVPPPDVTHHTSPGSPMQILHDLHHPINLSATTLTTSTTLNGSMTSASTHGTLPRQPQSILKDPNRNKQQQQQQHQQQQQLLNASLVGVGVPVSGPVGSLQILNLPAVGPGLGNNLLMTTSATYDPSGVGPMSSFNAANVAAVSQGLSCPVAYTDADGHLV

Target ORF information:

RefSeq Version	XM_023177252.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni nephrin (LOC6638412), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023177252.1

ORF Insert Sequence:

ATGGCATTGA AAACAGAAAT GACTGCAGAG GAACAACAAC AGCAGCAGCA GCAGCAACAT 
ACAACAAAAA GACGTCGACG GCGTTGCAAA GAACAGTGGA TTAAAAATCA TTTACAATTG 
CTATGCATAG TTGCCCTCTA TCTATGGCCA TGGTCATTGC CCTATGCTCG AGCCCAGCAA 
CAGAAATTCC GCACAACGCC TCATGATCTT CAAGTTCTGG AGGGTGCCGA GGCCATGATG 
CGCTGTGAAG TCACAAATTT GGCAGGCGCT GTTCAATGGA CGAAAGATGG CTTCGCCTTG 
GGCTTTGAGG CAGTCATACC GGGATTTCCA CGCTACTCCG TGCTCGGAGA TCGCAAACAG 
GGTGTCTATA ATTTGCGCAT ATCGAATGCC TCAATCAACG ATGATGCCGA ATATCAATGT 
CAAGTCGGTC CGGCGAGATT ACAATCGGCC ATCAGAGCGA ATGCCAAACT CACCGTCATA 
TCTCCACCGT CATCGATCGA AATCAAGGGT TATAGTCACA ACTCGAAGGT TGAAGTGCGT 
GAGAATCAGG ACTTGCAACT CAAATGCATT GTGGCCAATG CAAAGCCAGC GGCTCAAATA 
GTCTGGTATC GCGGCAATAC TGAATACAAA CCAGAAAAAC GGGAAGATAA GGTTGAAGAA 
TCGACGCCAA AACGATTTAC CACCACATCC ATTTTGAAGT TGAAACCAGG TCCTGATGAC 
GATTATACGG AATATACGTG CCAGGCCAAA CATAAGGCTT TATCGCCGGA TATGCCAATG 
CGTGCAACGG TTCAACTGTC CGTGTTGTAT CCACCTGGTC CACCCTATAT TGAAGGTTAT 
TCAGCTGGTG AAACATTGCG CCGTGGCCAA ACAGTGGAGC TTATGTGCCG CAGTCGTGGT 
GGCAATCCTC CAGCTCAACT TATTTGGTAT AAAAATGGCT CACAAATACG CATGGCCTAT 
AGAACCTCTG GACGTTTGTC GGAGAATATT TATACTTTTA CTGCCGAAGC GGGGGATAAT 
AAGGCTCGTT TTCGCTGCGA GGCCAGCAAT GTGATGTCAA AGGATCCCCT TAAAGCTGAA 
GTGGAATTAT CTGTTCTATT TGCACCTTCA CACGTCACCG TTACGGGTCC CACGGAGGCT 
CGTGTGGGGG ATGTTGTGCC ACTTACGTGC ACCACAGCAC CATCGAATCC ACCCGCTGAG 
ATCAAATGGA TGGTGGGCGG ACGTCAGATA CGCAATGCCA CATCAAAGAC CATTGTCAGT 
CCCGAAGGCG GCTGGACTAC TTCATCCAAT ATTAGCACTA TTGTGGAGCC AAATAAACGT 
TCCTTGGTAA TCATTTGCCA TGGCCTTAAT ATGCAGCTAA CAGAGAATGT TGTCTCCACC 
CATACGATCA ATGTCCTGTA TCCACCCGCA CCGCCATTAA TTTCTGGCTA TATAGAAGGA 
CAAATTATAC CAGCTGGTTC AGTGCAAAAA CTGCTTTGCG TTTCATCAGG CGGCAATCCG 
TTGGCAACGT TAACGTGGTA TAAAAACGAC AAAAGGATCA ACTCGGTTTT ACGCGCCGCG 
GACAAATCGG TTTCAGCGGA AATAACCATC TTGGCCAACG TTACCGACAA CCAGGCGCAA 
TATCGTTGCG AGGCCTCGAA TAGCGCCACG GAGATACCGC TCTTCCAGTC CACAACGCTC 
AGTGTGCATT TTGCCCCGGA GACGGTAAAA ATACGCATTG AACCGGAAGA GCTGCGACCT 
GGCATGGAGG CCTCCATCAT TTGCGACTCG AGCTCAAGCA ATCCGCCGGC CAAGCTGTCC 
TGGTGGAAGG ACGGCATCTC CATTGAAGGC ATTAATAACA CGTCCAAGCC CGGACTCTGG 
GGCGGCACAG TCTCTACGCT CGAATTCAGG GTAAACATCT CACAGGAAAT GAACGGCATA 
GTCTACACTT GCCAAAGCGC CAACGAGGCA CTACAACGTA GTGTCCATGA GGCCATAAGC 
CTGGATGTTC TATATCGTCC CAAGTTTGTG CCGCCGCCAT CCTCAACGGC TGTCGGTGTG 
GAAGGTGAAT CCCTGCAGGT GTCGCTCCAG GCGAATGGTA ATCCTGCAGC CATTGCCTAT 
ACATGGACAA AGGATGGCAT GAGTATACCC CAAAGCGATG GCGTTGAGCA TCGTATATTT 
GCCGAAGCGG GCACTTTGAA TTTTACCCGC CTGCATCGTG ACGATGCGGG GGTCTATTCC 
TGTAGTGCTG GCAACTCGCA AGGCAAGGCT GCACTCAATA TCACAGTGGT AGTGGAATAT 
GGCACTACCA TCAAGTCTAT ATCAGAGAAC ATCATTGTGA ATCCTGGTGA GGAGGCAATG 
CTTTCGTGTA CTGTTGAGGG TAAACCTCTA ACAGAGGAGC ATGTCAAGTG GGAACGAGTT 
GGCTACGATA TGGCCGCCAA GACAACAACA ACGTTTGCCA ATGGCACATC CTATCTGCAC 
ATTAAGGATG CTCAACGCGA AGATGTTGGT AACTTTCGTT GTGTGGCCGA CAATCGTGTG 
GCCAATCCCA CCAATCGAGA TGTCCTGCTG ATAGTCAAGT TTGCTCCGGA AATAGCCAAG 
TCACCGACAC TGCTGAGAGC TGCCAGCGGT ACAGGTGAGC GTGGTCGTTT ACCGTGTCGT 
GCGCAGGCAT CCCCGAAGCC AGAATTCATT TGGCGCCAGG ATAAAAAAGA TTTGCCCATT 
AATCGTACAT ATAAATATGA AGTGGAAGAG CGTAAAATCG ACTCATTGAC CTATGAATCT 
ACGCTAATTG TACAAAAGGT GGCTCCCGCC GACTACGGGG CCTACGAATG TGTGGCCCGC 
AATGAACTAG GCGAAACGAT AGAGACCGTC CGCTTGGATA TCACATCACC ACCAGATCCA 
CCACTTAGTC TCAATATCTT GAATGTTACC CACGATACAG TGACTGTTGC CTGGACGCCT 
GGTTTTGATG GCGGCCTAAA GGCCTCGTAT CGTGTGCGCT ATCGGATGGC CGACCGTGAG 
CAATACAAGT ACATTGATGG ATTGCCCAAT AGCCATAAGC TAACGATTGC TGGATTGCGT 
ATGAACACAC TTTACTTGTT CTCCGTCATG TCAAAGAATG AGTTGGGTCA GAGTGTCTAT 
TTGCCGGATT TAACGCGTGC CCAGACCAAA GAAGCACCAC CACCGTCACA GCCAGCCTCA 
TCACTGGGCG GTGGACCACC GACCACCAGT CAGACACCAT TGGGTGGTAC ATCGGGAATG 
TTGCTGTTTG TTGGTGTTTC GGCTGGCATT GCTGTCGTTG TAGTCAACAT TCTTGTCATC 
GGCTGTTGCC TGCATAAACG CAACGAGAAG CGCCTAAAGC GAGGACTTGA AATGCTGCCA 
GCGGAGTTAA CTGAGGACTC TAGCAATACA CCGAATCTGG TCATAATTGG CATATCGCTG 
GCGGCATTTG GCTTTCTCCT GGTCAATGCG GCGCTTGTTG CCTGGTTCTT TGTGCATCAG 
CGGCGCAAGA AAGTGGCGGA AACTACAAAT CAACCAGGCA AAACGGCAAC CATCGAAATG 
TATGCCCCCA GTTCGTACAA TGACACCGTT ACCGGCGAGA CACTGTCCAG TGTGTCGGAA 
AAGAGCGAAT CCTACTCGAA TGAGGGCAGC ACTCAGCCGG AATATATTGA CGAGGCTCGT 
AAGAAGGCTG CCTCAACATA TTTGGTTGAA GGGACAGCAG ATATGCCACC GCCCAGATAT 
CAAAAGGATG GCACTTTGCC AGTATTATAT CCGAATAATA TGGTAAATGC CTGCACCCTG 
CCACATCCGA GGCATAACAA TGCAATTGGA GCAACAGCAG CTGGTAGCAA TAATGCTGCT 
GCAGCCATCC ATATGACGCG AGACGATCAG ATGCTCATCA CCAAGGGCGT TTATATTCCG 
TCCCCATCGC CAGCGCCACC GCCAGATGGT TCCTATTATA ATATGAATAG CGATAGATAT 
CTATCCTATC CGCCAATGGA ATATACGGCT GCCTTGGACT TTAGTGCCCC ACCGTTGCCC 
ATGACGCATC TGCAACCCAT TACGGTCACC TCGCAAGCCA TGTTGACCTC AAATGGCGGT 
GCCACCCTGA TAGCCAATGG CAATGCAGCC AATGCAGGTG TTGGTGGTGG TGGTTCAGGC 
ACTCTGAGAC GTGGAATTCT GCGTGGAGTT GTGGGTGTAC CACCACCGGA TGTCACCCAT 
CACACCTCAC CGGGCAGTCC CATGCAAATA TTGCATGATC TGCATCATCC CATCAATTTG 
AGTGCCACAA CGTTAACAAC TAGCACCACA CTAAATGGCA GCATGACATC AGCATCAACG 
CATGGGACAC TGCCACGTCA ACCGCAGAGT ATCCTAAAGG ACCCAAATCG AAATAAGCAG 
CAACAGCAGC AACAACATCA GCAGCAGCAG CAGCTTCTCA ATGCCAGCCT AGTGGGTGTT 
GGTGTTCCTG TCTCAGGTCC GGTGGGGAGC CTGCAGATAC TGAATCTGCC CGCTGTGGGT 
CCTGGCCTGG GTAACAATCT ATTAATGACT ACCAGCGCCA CTTATGATCC AAGTGGCGTT 
GGACCTATGA GTAGTTTTAA TGCTGCCAAT GTGGCAGCTG TCTCACAGGG TCTTTCCTGT 
CCGGTGGCCT ATACAGATGC GGATGGACAT CTTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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DWIL\GK20677 cDNA ORF clone, Drosophila willistoni

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