Dpse\GA20388 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20388
Entrez Gene ID	4814079
Full Name	GA20388 gene product from transcript GA20388-RB
Synonyms	GA20388,dpse_GLEANR_1530
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354177.3	XP_001354213.2	uncharacterized protein, isoform A
XM_015186933.1	XP_015042419.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20388 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20388 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe08984	XM_015186933.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20388), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe08984	XM_001354177.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20388), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20388 ( XM_001354177.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354213.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20388), mRNA.

Price & Availability↑

CloneID	ODe08984
Clone ID Related Accession (Same CDS sequence)	XM_001354177.3 , XM_015186933.1
Accession Version	XM_001354177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589957). On Dec 22, 2015 this sequence version replaced XM_001354177.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATC CAATCTTTAA GCAAAAATAT GAAAAGTACA AGCTGCGCGT CAAGTTATGG 
GAGAGGGACT TTAAGAAGAA GCATGGCCGT GTGCCCTCCA AGTACGACAT ACGCGAGGCC 
GGCCAGGACA TCCGCGACTC CTACAAGATG TATTACAAGC TCAAGACTTC ATTTCTGGAG 
GAGACCCTCA ACGACGTGCT CAGCGAGGAT GGGTTCGACG TACTGGAAAT GTCGCAGGCA 
AGTGATCTGG GTGTCACCCT GCTGGACAGC GATATCAGCA TGCAGAATGC ACCCCAACTA 
CCGATGGATA TATCATCTAT TGTGAATCAG CAAGCGCAGA GTGGAGGATT CTCCAATCTT 
GAGGAGTTGC CAAATCCGCA GGCCCTAACC AATCTCGTAA ATCGTGATGA AAATCATGTG 
ATAAGGAAAT TCGAAGCCGT GGAAGAGCTG CAGCTCAATC AAAATGCCTG GGGCACAGAT 
GTAAGCAAGA AGCAGCAGCC AGAGCCATTG GGCTCTTCAA TACCACACGG TTCTGCTCAG 
CCAAAACAGC AGGCGGCCAG CTTAAAGCCT AGCCTTAGCG CCAAACTATT TCAATCCACA 
CGCGGCTTTG CCAAGCGCAA TCCCCGCAAG CCACTCTCAC GCTGTTCCTC CAATGTGGGT 
GCCTCTGTCT CATCTGGTCG GCCAGGGCAC GACGATCTCG CCGAAGAGCT GCCCGACTTT 
GAGACGATTC TCATACGCAA GGCGCAGGAA TACAAAGAGA AAGAGTTGGC CATGATTGCC 
AATCCACATC TTGCCACGGA TCAGCCCAAA GATGCCATCA AGACACAGGT GGACGAAGGC 
TGGCTGCAGA GGAACACTAT GGAAAACACT CTTGACGCTG ATATGCACAG CCCAGAGCTA 
GAGCCCAATA ACAATGCCGA TCATTTGCCC ACGAAAGTCA ACTACGGCCT GGCGAACATC 
GATCTGAGCA TGCTGAGGCA AAGCAGGAGT TTGGAAGAGC ACCAGCAGCA GCCTGAAGTG 
AAGGCAGAGC CAAAGCAAGT GGCAGACACG GACGTGGTGG CGTTACCATC TGTTGAGCAG 
GAAGCGGACA GCGATTCCGA CTCTGTTGTG GCCGAAAGTG ACGAGGAGCC GGAACAGCAG 
GAGTATCGAC AATTAGCCAA GCGACGAAAG GTTGTTCCTG AGGTAGAGCC AGAGGTACAC 
GAAGCAGAAG CAGAAGCAAG CTCGGAACCG ATGGAAATGT CCTTACCGGA CCCCGAAGAA 
GAGACCATGC TCAATGACTT CTCTGCGGAT GATGAAGAAC AAGATGCCAC CTACGAGCCG 
GACGGAGTAA AGAAAGGCAA CGTAAAGCGT AAGCAAGCAG CTGGCAAAAG ACAAACCACC 
CAGAAGCCCA AAGCAGAAAA GAAGCCAAAG GCAGAGAAGC AGCTAAAAGC AGAGAAGAAG 
CCAAAAGCAG AGAAGAAGCC GAAGGCAGAG AAAAAGCCAA TGGCTGAGAA GAAGACCAAG 
TCAAAGAAAG CTCCTCCAGC TGCAGAGTCA GTGCCCGAAC AAGAGGAAGA AGAACAGAGC 
CAGCCCCTGA ATCCAGAAGA TCTCAAATAT ATGCTTGCCC TGGAGGCGGG CGATGTAACC 
TCCGTGCCAC GCATCAATCC CCGGGAACTG GAACTGGTAG ACGAAACAGC CCAGCGCTAT 
ATAAGTACCT TTGCCACGCC CACAATGGCC GGAGGCGGAG GCAACTTCCC AGTGAATGAG 
AAGCGAGCTG CAGCCAGAAA AAAGCTAGAG GAGCGTGTGG CCTCGGGCAA ACTGAACGAG 
AACTTTGTGA CCATCAACAT ACAGAAAAAG AAATTTGTGA GAGGTAAAAA ATCGGTGAAT 
TTTACCAAAT ACAAGAAGCA GCAATGGAGG CACAAGAAGC ACATAGCAGC ATTGACTGGC 
CCCGACATGG ACATGGGTGG CTGTGATGGT GGAGTGCTCA CCTGCTTCCA GTGCGGTGGC 
GTCGGACACT TTGCCCAGCA GTGCAAGATG AAGGGCGACA GTCTTCTACC GCTTTCCGCT 
CAGTTGGAAG AGGATCCCTC GCCGTTTCCC ACGCTGGCCG AGGCCCAGGA AATGGCCTCG 
CAGGGCGCCC TGGTGGCCCA CAGTCGCAAC ATTGAGCGAC TGCCCCACGC GGCCAATGCG 
GCCATTCTAC AAATAGACGA GATGGAGGAC TCGGATCAGA GTGAGGAGAG CCACAGCGAT 
GGGGAGAGGC AGCCTCGACC AGAGCCAGAC TGGTCGAGCG ATGATCCGGA TATTGATTTT 
GAGGCCTTAG ATGCTGCTGT GGAAGCTTCG CAGGCTGAGG CTCCGATCTC ACCCATTAAG 
ACCTACGTGG GGCACAAGAT ACCCGAAGAG TTCCTCAAAC AGGCTGGCCT GGATGGCGCC 
TCCACTTCGA CGACTGGGGG CTCCAGTCGA AGCAAGCATG GCGGAGTGCA GCCACTGTAT 
GATCTTCTGC CCGACGGCAG TGTTCAGGCT GCAACACCAG AGGTACTGGA GGCCCTACAC 
ATGTTTGGTC ACAGCAACTT TCGTAAAGGT CAGGATCGTG CCGTTATGCG CACACTCTCT 
GGACTCTCCT CTTTGGTCAC CCTAAGCACT GGCAGTGGCA AGTCGTTGTG CTACCAATTG 
CCCGCGTATC TGTACAATCG GCAGAGGGGT GCCATAACGC TGGTCATCTC GCCGCTGGTG 
TCGCTGATGG AGGATCAGGT GACGGGGGTG CCGCAGTTTC TTCGCGCGCA CTGCCTGCAC 
ACCAACCAGA CGCCCCAGCA GCGCATCAAG GTGCAGGAGA TGATTGCCAA CGGGGAGATT 
GATATTCTCC TGGTGTCGCC CGAGGCCGTT GTGTCGGGGG AGCGTGCCAC AGGGTTTGGG 
GCCATTCTGC GTAAGCTGCC GCCCATTGCC TTTGCGTGCA TCGACGAGGC ACATTGCGTG 
TCCCAGTGGA GCCATAATTT TCGACCCAGC TACCTGATGA TCTGCAAGGT CTTGAAGAAG 
AATCTTGGCG TGCAGACAGT GTTGGGTCTC ACAGCGACGG CCACGCTGCC CACGAGGGTG 
AGCATCATCA ATCACTTGGG CATCACGGAT GGGGAGCGGG GCATCATTAG TGACACTCCC 
TTGCCAGATA ATCTGGTCCT GTCCGTGTCC AAGGATGAAA ATCGCGATGC GGCACTGCTG 
CAACTGCTCA ACAGTGAGAG ATTCGAGTCC TGCCAGTCGA TCATCATCTA CTGCACGCGT 
CGTGATGAGT GTGAGCGGAT TGCCGGTTTC ATACGCACCT GTGTGCAGGA TCGCAGGAAA 
CCAGATGGAG CCCAGGAACA GGGGAAGAAG CGCAAGCGTG TGAACTGGCA GGCCGAGCCG 
TACCATGCGG GAATGCCCGC CTCGCGGCGC CGCACTATCC AGAAGGCCTT TATGGGGAAT 
GAGCTGCGCA TTGTGGTGGC CACCATTGCC TTTGGCATGG GCATCAATAA GCCGGACATT 
CGGGCTGTTA TCCACTACAA TATGCCGCGC AATTTCGAAA GTTATGTGCA GGAGATCGGA 
CGCGCTGGTC GCGATGGCTT GCATGCCCAC TGCCATCTCT TTCTGGACGC GAGGGGCGGC 
GACCAGGGCG AGCTGCGGCG TCATGTGTAC GCCAATTCCA TTGATCGCCA TGTGATAAGG 
AAGCTGCTGC AAAAGATCTT TGTACCCTGC AGCTGTGAGA AGCAATTGCC GAAAGGAACA 
GAACCGCAAG CCGCAGCTAT AGAAGCAGAC GGAACACGGT ACCACATCTG TCCTGGGCAT 
GAGATTGGCT TCTCTGTGGA GAAGACCGTT GAAGCTCTGG ACATTCCCGC CGAGAATATA 
TCAACGCTGC TCTGCTACAT GGAACTTGAG CCGCGCTGGT GCATAAATGT CCTCAGCTCA 
GCGTATATCA TGGCCAAGGT GATCTCCTAC GGCGGCCCAA AGTATTTAAA GCATGCTGCC 
AAAGAGTGTC CGCCACTGGC CATGGCCATT GCCCTGCAGA TTAAGGGCAA GACCTTCAAG 
GATGACTCTA ATATCATCGA GTTTTCGGTG ACGGATATTG CCGCCGGCAT TGGGTGGAAC 
AGCGGTGTGG TCAAGTACCA GCTGAAGGAC CTGGAATGGA TAAAAGTTGG TGGATATCCA 
AAGCGTTCGC CCATCACTGT CAGTTTCTAT GATTTGGGCT TCCGCATTAA GGTGCCCGGT 
GACTTCACCG AAACGGAGAT TGATAATGCA CTGGATACGC TCTATACGCG CTCCGTCAAG 
CAGGAGAAGA CACAGCTGAT TCAGCTGCAG TACGTAGCCC ATGGCCTGGC TGCCGTAGCC 
TACAGTTCGT GTCACCACTG CTGTAGTGCG GACTTTCCCC AGGACCGATG CACACAACTG 
AAGGCCATTG TGAGGAATTA CTTCCTCAAC GACTATCCGC AGGATCTTGA ACTGGAAATC 
GAGCCCAGCA ATGTGCCGGA TGAAAGCATC GTTGATGATG TTCATTCCCT GATCAACATG 
TACCCCGACA ACACATTCAC TGGACGAAAC ATTGCCCGCA TATTCCATGG CATCACGAGT 
CCAAATTACC CGGCCGTCAT GTGGGGACGT TGCAAGTTCT GGCGCGCCCA CATGAAAGTC 
GACTTCAATC GTATTCTCCA GTTGGCCAAT CAAGAGATTA TCAAACGGCG GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354213.2
CDS	135..4811
Translation	MDDPIFKQKYEKYKLRVKLWERDFKKKHGRVPSKYDIREAGQDIRDSYKMYYKLKTSFLEETLNDVLSEDGFDVLEMSQASDLGVTLLDSDISMQNAPQLPMDISSIVNQQAQSGGFSNLEELPNPQALTNLVNRDENHVIRKFEAVEELQLNQNAWGTDVSKKQQPEPLGSSIPHGSAQPKQQAASLKPSLSAKLFQSTRGFAKRNPRKPLSRCSSNVGASVSSGRPGHDDLAEELPDFETILIRKAQEYKEKELAMIANPHLATDQPKDAIKTQVDEGWLQRNTMENTLDADMHSPELEPNNNADHLPTKVNYGLANIDLSMLRQSRSLEEHQQQPEVKAEPKQVADTDVVALPSVEQEADSDSDSVVAESDEEPEQQEYRQLAKRRKVVPEVEPEVHEAEAEASSEPMEMSLPDPEEETMLNDFSADDEEQDATYEPDGVKKGNVKRKQAAGKRQTTQKPKAEKKPKAEKQLKAEKKPKAEKKPKAEKKPMAEKKTKSKKAPPAAESVPEQEEEEQSQPLNPEDLKYMLALEAGDVTSVPRINPRELELVDETAQRYISTFATPTMAGGGGNFPVNEKRAAARKKLEERVASGKLNENFVTINIQKKKFVRGKKSVNFTKYKKQQWRHKKHIAALTGPDMDMGGCDGGVLTCFQCGGVGHFAQQCKMKGDSLLPLSAQLEEDPSPFPTLAEAQEMASQGALVAHSRNIERLPHAANAAILQIDEMEDSDQSEESHSDGERQPRPEPDWSSDDPDIDFEALDAAVEASQAEAPISPIKTYVGHKIPEEFLKQAGLDGASTSTTGGSSRSKHGGVQPLYDLLPDGSVQAATPEVLEALHMFGHSNFRKGQDRAVMRTLSGLSSLVTLSTGSGKSLCYQLPAYLYNRQRGAITLVISPLVSLMEDQVTGVPQFLRAHCLHTNQTPQQRIKVQEMIANGEIDILLVSPEAVVSGERATGFGAILRKLPPIAFACIDEAHCVSQWSHNFRPSYLMICKVLKKNLGVQTVLGLTATATLPTRVSIINHLGITDGERGIISDTPLPDNLVLSVSKDENRDAALLQLLNSERFESCQSIIIYCTRRDECERIAGFIRTCVQDRRKPDGAQEQGKKRKRVNWQAEPYHAGMPASRRRTIQKAFMGNELRIVVATIAFGMGINKPDIRAVIHYNMPRNFESYVQEIGRAGRDGLHAHCHLFLDARGGDQGELRRHVYANSIDRHVIRKLLQKIFVPCSCEKQLPKGTEPQAAAIEADGTRYHICPGHEIGFSVEKTVEALDIPAENISTLLCYMELEPRWCINVLSSAYIMAKVISYGGPKYLKHAAKECPPLAMAIALQIKGKTFKDDSNIIEFSVTDIAAGIGWNSGVVKYQLKDLEWIKVGGYPKRSPITVSFYDLGFRIKVPGDFTETEIDNALDTLYTRSVKQEKTQLIQLQYVAHGLAAVAYSSCHHCCSADFPQDRCTQLKAIVRNYFLNDYPQDLELEIEPSNVPDESIVDDVHSLINMYPDNTFTGRNIARIFHGITSPNYPAVMWGRCKFWRAHMKVDFNRILQLANQEIIKRRT

Target ORF information:

RefSeq Version	XM_001354177.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20388), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354177.3

ORF Insert Sequence:

ATGGACGATC CAATCTTTAA GCAAAAATAT GAAAAGTACA AGCTGCGCGT CAAGTTATGG 
GAGAGGGACT TTAAGAAGAA GCATGGCCGT GTGCCCTCCA AGTACGACAT ACGCGAGGCC 
GGCCAGGACA TCCGCGACTC CTACAAGATG TATTACAAGC TCAAGACTTC ATTTCTGGAG 
GAGACCCTCA ACGACGTGCT CAGCGAGGAT GGGTTCGACG TACTGGAAAT GTCGCAGGCA 
AGTGATCTGG GTGTCACCCT GCTGGACAGC GATATCAGCA TGCAGAATGC ACCCCAACTA 
CCGATGGATA TATCATCTAT TGTGAATCAG CAAGCGCAGA GTGGAGGATT CTCCAATCTT 
GAGGAGTTGC CAAATCCGCA GGCCCTAACC AATCTCGTAA ATCGTGATGA AAATCATGTG 
ATAAGGAAAT TCGAAGCCGT GGAAGAGCTG CAGCTCAATC AAAATGCCTG GGGCACAGAT 
GTAAGCAAGA AGCAGCAGCC AGAGCCATTG GGCTCTTCAA TACCACACGG TTCTGCTCAG 
CCAAAACAGC AGGCGGCCAG CTTAAAGCCT AGCCTTAGCG CCAAACTATT TCAATCCACA 
CGCGGCTTTG CCAAGCGCAA TCCCCGCAAG CCACTCTCAC GCTGTTCCTC CAATGTGGGT 
GCCTCTGTCT CATCTGGTCG GCCAGGGCAC GACGATCTCG CCGAAGAGCT GCCCGACTTT 
GAGACGATTC TCATACGCAA GGCGCAGGAA TACAAAGAGA AAGAGTTGGC CATGATTGCC 
AATCCACATC TTGCCACGGA TCAGCCCAAA GATGCCATCA AGACACAGGT GGACGAAGGC 
TGGCTGCAGA GGAACACTAT GGAAAACACT CTTGACGCTG ATATGCACAG CCCAGAGCTA 
GAGCCCAATA ACAATGCCGA TCATTTGCCC ACGAAAGTCA ACTACGGCCT GGCGAACATC 
GATCTGAGCA TGCTGAGGCA AAGCAGGAGT TTGGAAGAGC ACCAGCAGCA GCCTGAAGTG 
AAGGCAGAGC CAAAGCAAGT GGCAGACACG GACGTGGTGG CGTTACCATC TGTTGAGCAG 
GAAGCGGACA GCGATTCCGA CTCTGTTGTG GCCGAAAGTG ACGAGGAGCC GGAACAGCAG 
GAGTATCGAC AATTAGCCAA GCGACGAAAG GTTGTTCCTG AGGTAGAGCC AGAGGTACAC 
GAAGCAGAAG CAGAAGCAAG CTCGGAACCG ATGGAAATGT CCTTACCGGA CCCCGAAGAA 
GAGACCATGC TCAATGACTT CTCTGCGGAT GATGAAGAAC AAGATGCCAC CTACGAGCCG 
GACGGAGTAA AGAAAGGCAA CGTAAAGCGT AAGCAAGCAG CTGGCAAAAG ACAAACCACC 
CAGAAGCCCA AAGCAGAAAA GAAGCCAAAG GCAGAGAAGC AGCTAAAAGC AGAGAAGAAG 
CCAAAAGCAG AGAAGAAGCC GAAGGCAGAG AAAAAGCCAA TGGCTGAGAA GAAGACCAAG 
TCAAAGAAAG CTCCTCCAGC TGCAGAGTCA GTGCCCGAAC AAGAGGAAGA AGAACAGAGC 
CAGCCCCTGA ATCCAGAAGA TCTCAAATAT ATGCTTGCCC TGGAGGCGGG CGATGTAACC 
TCCGTGCCAC GCATCAATCC CCGGGAACTG GAACTGGTAG ACGAAACAGC CCAGCGCTAT 
ATAAGTACCT TTGCCACGCC CACAATGGCC GGAGGCGGAG GCAACTTCCC AGTGAATGAG 
AAGCGAGCTG CAGCCAGAAA AAAGCTAGAG GAGCGTGTGG CCTCGGGCAA ACTGAACGAG 
AACTTTGTGA CCATCAACAT ACAGAAAAAG AAATTTGTGA GAGGTAAAAA ATCGGTGAAT 
TTTACCAAAT ACAAGAAGCA GCAATGGAGG CACAAGAAGC ACATAGCAGC ATTGACTGGC 
CCCGACATGG ACATGGGTGG CTGTGATGGT GGAGTGCTCA CCTGCTTCCA GTGCGGTGGC 
GTCGGACACT TTGCCCAGCA GTGCAAGATG AAGGGCGACA GTCTTCTACC GCTTTCCGCT 
CAGTTGGAAG AGGATCCCTC GCCGTTTCCC ACGCTGGCCG AGGCCCAGGA AATGGCCTCG 
CAGGGCGCCC TGGTGGCCCA CAGTCGCAAC ATTGAGCGAC TGCCCCACGC GGCCAATGCG 
GCCATTCTAC AAATAGACGA GATGGAGGAC TCGGATCAGA GTGAGGAGAG CCACAGCGAT 
GGGGAGAGGC AGCCTCGACC AGAGCCAGAC TGGTCGAGCG ATGATCCGGA TATTGATTTT 
GAGGCCTTAG ATGCTGCTGT GGAAGCTTCG CAGGCTGAGG CTCCGATCTC ACCCATTAAG 
ACCTACGTGG GGCACAAGAT ACCCGAAGAG TTCCTCAAAC AGGCTGGCCT GGATGGCGCC 
TCCACTTCGA CGACTGGGGG CTCCAGTCGA AGCAAGCATG GCGGAGTGCA GCCACTGTAT 
GATCTTCTGC CCGACGGCAG TGTTCAGGCT GCAACACCAG AGGTACTGGA GGCCCTACAC 
ATGTTTGGTC ACAGCAACTT TCGTAAAGGT CAGGATCGTG CCGTTATGCG CACACTCTCT 
GGACTCTCCT CTTTGGTCAC CCTAAGCACT GGCAGTGGCA AGTCGTTGTG CTACCAATTG 
CCCGCGTATC TGTACAATCG GCAGAGGGGT GCCATAACGC TGGTCATCTC GCCGCTGGTG 
TCGCTGATGG AGGATCAGGT GACGGGGGTG CCGCAGTTTC TTCGCGCGCA CTGCCTGCAC 
ACCAACCAGA CGCCCCAGCA GCGCATCAAG GTGCAGGAGA TGATTGCCAA CGGGGAGATT 
GATATTCTCC TGGTGTCGCC CGAGGCCGTT GTGTCGGGGG AGCGTGCCAC AGGGTTTGGG 
GCCATTCTGC GTAAGCTGCC GCCCATTGCC TTTGCGTGCA TCGACGAGGC ACATTGCGTG 
TCCCAGTGGA GCCATAATTT TCGACCCAGC TACCTGATGA TCTGCAAGGT CTTGAAGAAG 
AATCTTGGCG TGCAGACAGT GTTGGGTCTC ACAGCGACGG CCACGCTGCC CACGAGGGTG 
AGCATCATCA ATCACTTGGG CATCACGGAT GGGGAGCGGG GCATCATTAG TGACACTCCC 
TTGCCAGATA ATCTGGTCCT GTCCGTGTCC AAGGATGAAA ATCGCGATGC GGCACTGCTG 
CAACTGCTCA ACAGTGAGAG ATTCGAGTCC TGCCAGTCGA TCATCATCTA CTGCACGCGT 
CGTGATGAGT GTGAGCGGAT TGCCGGTTTC ATACGCACCT GTGTGCAGGA TCGCAGGAAA 
CCAGATGGAG CCCAGGAACA GGGGAAGAAG CGCAAGCGTG TGAACTGGCA GGCCGAGCCG 
TACCATGCGG GAATGCCCGC CTCGCGGCGC CGCACTATCC AGAAGGCCTT TATGGGGAAT 
GAGCTGCGCA TTGTGGTGGC CACCATTGCC TTTGGCATGG GCATCAATAA GCCGGACATT 
CGGGCTGTTA TCCACTACAA TATGCCGCGC AATTTCGAAA GTTATGTGCA GGAGATCGGA 
CGCGCTGGTC GCGATGGCTT GCATGCCCAC TGCCATCTCT TTCTGGACGC GAGGGGCGGC 
GACCAGGGCG AGCTGCGGCG TCATGTGTAC GCCAATTCCA TTGATCGCCA TGTGATAAGG 
AAGCTGCTGC AAAAGATCTT TGTACCCTGC AGCTGTGAGA AGCAATTGCC GAAAGGAACA 
GAACCGCAAG CCGCAGCTAT AGAAGCAGAC GGAACACGGT ACCACATCTG TCCTGGGCAT 
GAGATTGGCT TCTCTGTGGA GAAGACCGTT GAAGCTCTGG ACATTCCCGC CGAGAATATA 
TCAACGCTGC TCTGCTACAT GGAACTTGAG CCGCGCTGGT GCATAAATGT CCTCAGCTCA 
GCGTATATCA TGGCCAAGGT GATCTCCTAC GGCGGCCCAA AGTATTTAAA GCATGCTGCC 
AAAGAGTGTC CGCCACTGGC CATGGCCATT GCCCTGCAGA TTAAGGGCAA GACCTTCAAG 
GATGACTCTA ATATCATCGA GTTTTCGGTG ACGGATATTG CCGCCGGCAT TGGGTGGAAC 
AGCGGTGTGG TCAAGTACCA GCTGAAGGAC CTGGAATGGA TAAAAGTTGG TGGATATCCA 
AAGCGTTCGC CCATCACTGT CAGTTTCTAT GATTTGGGCT TCCGCATTAA GGTGCCCGGT 
GACTTCACCG AAACGGAGAT TGATAATGCA CTGGATACGC TCTATACGCG CTCCGTCAAG 
CAGGAGAAGA CACAGCTGAT TCAGCTGCAG TACGTAGCCC ATGGCCTGGC TGCCGTAGCC 
TACAGTTCGT GTCACCACTG CTGTAGTGCG GACTTTCCCC AGGACCGATG CACACAACTG 
AAGGCCATTG TGAGGAATTA CTTCCTCAAC GACTATCCGC AGGATCTTGA ACTGGAAATC 
GAGCCCAGCA ATGTGCCGGA TGAAAGCATC GTTGATGATG TTCATTCCCT GATCAACATG 
TACCCCGACA ACACATTCAC TGGACGAAAC ATTGCCCGCA TATTCCATGG CATCACGAGT 
CCAAATTACC CGGCCGTCAT GTGGGGACGT TGCAAGTTCT GGCGCGCCCA CATGAAAGTC 
GACTTCAATC GTATTCTCCA GTTGGCCAAT CAAGAGATTA TCAAACGGCG GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA20388 ( XM_015186933.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042419.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20388), mRNA.

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CloneID	ODe08984
Clone ID Related Accession (Same CDS sequence)	XM_001354177.3 , XM_015186933.1
Accession Version	XM_015186933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589957). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATC CAATCTTTAA GCAAAAATAT GAAAAGTACA AGCTGCGCGT CAAGTTATGG 
GAGAGGGACT TTAAGAAGAA GCATGGCCGT GTGCCCTCCA AGTACGACAT ACGCGAGGCC 
GGCCAGGACA TCCGCGACTC CTACAAGATG TATTACAAGC TCAAGACTTC ATTTCTGGAG 
GAGACCCTCA ACGACGTGCT CAGCGAGGAT GGGTTCGACG TACTGGAAAT GTCGCAGGCA 
AGTGATCTGG GTGTCACCCT GCTGGACAGC GATATCAGCA TGCAGAATGC ACCCCAACTA 
CCGATGGATA TATCATCTAT TGTGAATCAG CAAGCGCAGA GTGGAGGATT CTCCAATCTT 
GAGGAGTTGC CAAATCCGCA GGCCCTAACC AATCTCGTAA ATCGTGATGA AAATCATGTG 
ATAAGGAAAT TCGAAGCCGT GGAAGAGCTG CAGCTCAATC AAAATGCCTG GGGCACAGAT 
GTAAGCAAGA AGCAGCAGCC AGAGCCATTG GGCTCTTCAA TACCACACGG TTCTGCTCAG 
CCAAAACAGC AGGCGGCCAG CTTAAAGCCT AGCCTTAGCG CCAAACTATT TCAATCCACA 
CGCGGCTTTG CCAAGCGCAA TCCCCGCAAG CCACTCTCAC GCTGTTCCTC CAATGTGGGT 
GCCTCTGTCT CATCTGGTCG GCCAGGGCAC GACGATCTCG CCGAAGAGCT GCCCGACTTT 
GAGACGATTC TCATACGCAA GGCGCAGGAA TACAAAGAGA AAGAGTTGGC CATGATTGCC 
AATCCACATC TTGCCACGGA TCAGCCCAAA GATGCCATCA AGACACAGGT GGACGAAGGC 
TGGCTGCAGA GGAACACTAT GGAAAACACT CTTGACGCTG ATATGCACAG CCCAGAGCTA 
GAGCCCAATA ACAATGCCGA TCATTTGCCC ACGAAAGTCA ACTACGGCCT GGCGAACATC 
GATCTGAGCA TGCTGAGGCA AAGCAGGAGT TTGGAAGAGC ACCAGCAGCA GCCTGAAGTG 
AAGGCAGAGC CAAAGCAAGT GGCAGACACG GACGTGGTGG CGTTACCATC TGTTGAGCAG 
GAAGCGGACA GCGATTCCGA CTCTGTTGTG GCCGAAAGTG ACGAGGAGCC GGAACAGCAG 
GAGTATCGAC AATTAGCCAA GCGACGAAAG GTTGTTCCTG AGGTAGAGCC AGAGGTACAC 
GAAGCAGAAG CAGAAGCAAG CTCGGAACCG ATGGAAATGT CCTTACCGGA CCCCGAAGAA 
GAGACCATGC TCAATGACTT CTCTGCGGAT GATGAAGAAC AAGATGCCAC CTACGAGCCG 
GACGGAGTAA AGAAAGGCAA CGTAAAGCGT AAGCAAGCAG CTGGCAAAAG ACAAACCACC 
CAGAAGCCCA AAGCAGAAAA GAAGCCAAAG GCAGAGAAGC AGCTAAAAGC AGAGAAGAAG 
CCAAAAGCAG AGAAGAAGCC GAAGGCAGAG AAAAAGCCAA TGGCTGAGAA GAAGACCAAG 
TCAAAGAAAG CTCCTCCAGC TGCAGAGTCA GTGCCCGAAC AAGAGGAAGA AGAACAGAGC 
CAGCCCCTGA ATCCAGAAGA TCTCAAATAT ATGCTTGCCC TGGAGGCGGG CGATGTAACC 
TCCGTGCCAC GCATCAATCC CCGGGAACTG GAACTGGTAG ACGAAACAGC CCAGCGCTAT 
ATAAGTACCT TTGCCACGCC CACAATGGCC GGAGGCGGAG GCAACTTCCC AGTGAATGAG 
AAGCGAGCTG CAGCCAGAAA AAAGCTAGAG GAGCGTGTGG CCTCGGGCAA ACTGAACGAG 
AACTTTGTGA CCATCAACAT ACAGAAAAAG AAATTTGTGA GAGGTAAAAA ATCGGTGAAT 
TTTACCAAAT ACAAGAAGCA GCAATGGAGG CACAAGAAGC ACATAGCAGC ATTGACTGGC 
CCCGACATGG ACATGGGTGG CTGTGATGGT GGAGTGCTCA CCTGCTTCCA GTGCGGTGGC 
GTCGGACACT TTGCCCAGCA GTGCAAGATG AAGGGCGACA GTCTTCTACC GCTTTCCGCT 
CAGTTGGAAG AGGATCCCTC GCCGTTTCCC ACGCTGGCCG AGGCCCAGGA AATGGCCTCG 
CAGGGCGCCC TGGTGGCCCA CAGTCGCAAC ATTGAGCGAC TGCCCCACGC GGCCAATGCG 
GCCATTCTAC AAATAGACGA GATGGAGGAC TCGGATCAGA GTGAGGAGAG CCACAGCGAT 
GGGGAGAGGC AGCCTCGACC AGAGCCAGAC TGGTCGAGCG ATGATCCGGA TATTGATTTT 
GAGGCCTTAG ATGCTGCTGT GGAAGCTTCG CAGGCTGAGG CTCCGATCTC ACCCATTAAG 
ACCTACGTGG GGCACAAGAT ACCCGAAGAG TTCCTCAAAC AGGCTGGCCT GGATGGCGCC 
TCCACTTCGA CGACTGGGGG CTCCAGTCGA AGCAAGCATG GCGGAGTGCA GCCACTGTAT 
GATCTTCTGC CCGACGGCAG TGTTCAGGCT GCAACACCAG AGGTACTGGA GGCCCTACAC 
ATGTTTGGTC ACAGCAACTT TCGTAAAGGT CAGGATCGTG CCGTTATGCG CACACTCTCT 
GGACTCTCCT CTTTGGTCAC CCTAAGCACT GGCAGTGGCA AGTCGTTGTG CTACCAATTG 
CCCGCGTATC TGTACAATCG GCAGAGGGGT GCCATAACGC TGGTCATCTC GCCGCTGGTG 
TCGCTGATGG AGGATCAGGT GACGGGGGTG CCGCAGTTTC TTCGCGCGCA CTGCCTGCAC 
ACCAACCAGA CGCCCCAGCA GCGCATCAAG GTGCAGGAGA TGATTGCCAA CGGGGAGATT 
GATATTCTCC TGGTGTCGCC CGAGGCCGTT GTGTCGGGGG AGCGTGCCAC AGGGTTTGGG 
GCCATTCTGC GTAAGCTGCC GCCCATTGCC TTTGCGTGCA TCGACGAGGC ACATTGCGTG 
TCCCAGTGGA GCCATAATTT TCGACCCAGC TACCTGATGA TCTGCAAGGT CTTGAAGAAG 
AATCTTGGCG TGCAGACAGT GTTGGGTCTC ACAGCGACGG CCACGCTGCC CACGAGGGTG 
AGCATCATCA ATCACTTGGG CATCACGGAT GGGGAGCGGG GCATCATTAG TGACACTCCC 
TTGCCAGATA ATCTGGTCCT GTCCGTGTCC AAGGATGAAA ATCGCGATGC GGCACTGCTG 
CAACTGCTCA ACAGTGAGAG ATTCGAGTCC TGCCAGTCGA TCATCATCTA CTGCACGCGT 
CGTGATGAGT GTGAGCGGAT TGCCGGTTTC ATACGCACCT GTGTGCAGGA TCGCAGGAAA 
CCAGATGGAG CCCAGGAACA GGGGAAGAAG CGCAAGCGTG TGAACTGGCA GGCCGAGCCG 
TACCATGCGG GAATGCCCGC CTCGCGGCGC CGCACTATCC AGAAGGCCTT TATGGGGAAT 
GAGCTGCGCA TTGTGGTGGC CACCATTGCC TTTGGCATGG GCATCAATAA GCCGGACATT 
CGGGCTGTTA TCCACTACAA TATGCCGCGC AATTTCGAAA GTTATGTGCA GGAGATCGGA 
CGCGCTGGTC GCGATGGCTT GCATGCCCAC TGCCATCTCT TTCTGGACGC GAGGGGCGGC 
GACCAGGGCG AGCTGCGGCG TCATGTGTAC GCCAATTCCA TTGATCGCCA TGTGATAAGG 
AAGCTGCTGC AAAAGATCTT TGTACCCTGC AGCTGTGAGA AGCAATTGCC GAAAGGAACA 
GAACCGCAAG CCGCAGCTAT AGAAGCAGAC GGAACACGGT ACCACATCTG TCCTGGGCAT 
GAGATTGGCT TCTCTGTGGA GAAGACCGTT GAAGCTCTGG ACATTCCCGC CGAGAATATA 
TCAACGCTGC TCTGCTACAT GGAACTTGAG CCGCGCTGGT GCATAAATGT CCTCAGCTCA 
GCGTATATCA TGGCCAAGGT GATCTCCTAC GGCGGCCCAA AGTATTTAAA GCATGCTGCC 
AAAGAGTGTC CGCCACTGGC CATGGCCATT GCCCTGCAGA TTAAGGGCAA GACCTTCAAG 
GATGACTCTA ATATCATCGA GTTTTCGGTG ACGGATATTG CCGCCGGCAT TGGGTGGAAC 
AGCGGTGTGG TCAAGTACCA GCTGAAGGAC CTGGAATGGA TAAAAGTTGG TGGATATCCA 
AAGCGTTCGC CCATCACTGT CAGTTTCTAT GATTTGGGCT TCCGCATTAA GGTGCCCGGT 
GACTTCACCG AAACGGAGAT TGATAATGCA CTGGATACGC TCTATACGCG CTCCGTCAAG 
CAGGAGAAGA CACAGCTGAT TCAGCTGCAG TACGTAGCCC ATGGCCTGGC TGCCGTAGCC 
TACAGTTCGT GTCACCACTG CTGTAGTGCG GACTTTCCCC AGGACCGATG CACACAACTG 
AAGGCCATTG TGAGGAATTA CTTCCTCAAC GACTATCCGC AGGATCTTGA ACTGGAAATC 
GAGCCCAGCA ATGTGCCGGA TGAAAGCATC GTTGATGATG TTCATTCCCT GATCAACATG 
TACCCCGACA ACACATTCAC TGGACGAAAC ATTGCCCGCA TATTCCATGG CATCACGAGT 
CCAAATTACC CGGCCGTCAT GTGGGGACGT TGCAAGTTCT GGCGCGCCCA CATGAAAGTC 
GACTTCAATC GTATTCTCCA GTTGGCCAAT CAAGAGATTA TCAAACGGCG GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042419.1
CDS	155..4831
Translation	MDDPIFKQKYEKYKLRVKLWERDFKKKHGRVPSKYDIREAGQDIRDSYKMYYKLKTSFLEETLNDVLSEDGFDVLEMSQASDLGVTLLDSDISMQNAPQLPMDISSIVNQQAQSGGFSNLEELPNPQALTNLVNRDENHVIRKFEAVEELQLNQNAWGTDVSKKQQPEPLGSSIPHGSAQPKQQAASLKPSLSAKLFQSTRGFAKRNPRKPLSRCSSNVGASVSSGRPGHDDLAEELPDFETILIRKAQEYKEKELAMIANPHLATDQPKDAIKTQVDEGWLQRNTMENTLDADMHSPELEPNNNADHLPTKVNYGLANIDLSMLRQSRSLEEHQQQPEVKAEPKQVADTDVVALPSVEQEADSDSDSVVAESDEEPEQQEYRQLAKRRKVVPEVEPEVHEAEAEASSEPMEMSLPDPEEETMLNDFSADDEEQDATYEPDGVKKGNVKRKQAAGKRQTTQKPKAEKKPKAEKQLKAEKKPKAEKKPKAEKKPMAEKKTKSKKAPPAAESVPEQEEEEQSQPLNPEDLKYMLALEAGDVTSVPRINPRELELVDETAQRYISTFATPTMAGGGGNFPVNEKRAAARKKLEERVASGKLNENFVTINIQKKKFVRGKKSVNFTKYKKQQWRHKKHIAALTGPDMDMGGCDGGVLTCFQCGGVGHFAQQCKMKGDSLLPLSAQLEEDPSPFPTLAEAQEMASQGALVAHSRNIERLPHAANAAILQIDEMEDSDQSEESHSDGERQPRPEPDWSSDDPDIDFEALDAAVEASQAEAPISPIKTYVGHKIPEEFLKQAGLDGASTSTTGGSSRSKHGGVQPLYDLLPDGSVQAATPEVLEALHMFGHSNFRKGQDRAVMRTLSGLSSLVTLSTGSGKSLCYQLPAYLYNRQRGAITLVISPLVSLMEDQVTGVPQFLRAHCLHTNQTPQQRIKVQEMIANGEIDILLVSPEAVVSGERATGFGAILRKLPPIAFACIDEAHCVSQWSHNFRPSYLMICKVLKKNLGVQTVLGLTATATLPTRVSIINHLGITDGERGIISDTPLPDNLVLSVSKDENRDAALLQLLNSERFESCQSIIIYCTRRDECERIAGFIRTCVQDRRKPDGAQEQGKKRKRVNWQAEPYHAGMPASRRRTIQKAFMGNELRIVVATIAFGMGINKPDIRAVIHYNMPRNFESYVQEIGRAGRDGLHAHCHLFLDARGGDQGELRRHVYANSIDRHVIRKLLQKIFVPCSCEKQLPKGTEPQAAAIEADGTRYHICPGHEIGFSVEKTVEALDIPAENISTLLCYMELEPRWCINVLSSAYIMAKVISYGGPKYLKHAAKECPPLAMAIALQIKGKTFKDDSNIIEFSVTDIAAGIGWNSGVVKYQLKDLEWIKVGGYPKRSPITVSFYDLGFRIKVPGDFTETEIDNALDTLYTRSVKQEKTQLIQLQYVAHGLAAVAYSSCHHCCSADFPQDRCTQLKAIVRNYFLNDYPQDLELEIEPSNVPDESIVDDVHSLINMYPDNTFTGRNIARIFHGITSPNYPAVMWGRCKFWRAHMKVDFNRILQLANQEIIKRRT

Target ORF information:

RefSeq Version	XM_015186933.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20388), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015186933.1

ORF Insert Sequence:

ATGGACGATC CAATCTTTAA GCAAAAATAT GAAAAGTACA AGCTGCGCGT CAAGTTATGG 
GAGAGGGACT TTAAGAAGAA GCATGGCCGT GTGCCCTCCA AGTACGACAT ACGCGAGGCC 
GGCCAGGACA TCCGCGACTC CTACAAGATG TATTACAAGC TCAAGACTTC ATTTCTGGAG 
GAGACCCTCA ACGACGTGCT CAGCGAGGAT GGGTTCGACG TACTGGAAAT GTCGCAGGCA 
AGTGATCTGG GTGTCACCCT GCTGGACAGC GATATCAGCA TGCAGAATGC ACCCCAACTA 
CCGATGGATA TATCATCTAT TGTGAATCAG CAAGCGCAGA GTGGAGGATT CTCCAATCTT 
GAGGAGTTGC CAAATCCGCA GGCCCTAACC AATCTCGTAA ATCGTGATGA AAATCATGTG 
ATAAGGAAAT TCGAAGCCGT GGAAGAGCTG CAGCTCAATC AAAATGCCTG GGGCACAGAT 
GTAAGCAAGA AGCAGCAGCC AGAGCCATTG GGCTCTTCAA TACCACACGG TTCTGCTCAG 
CCAAAACAGC AGGCGGCCAG CTTAAAGCCT AGCCTTAGCG CCAAACTATT TCAATCCACA 
CGCGGCTTTG CCAAGCGCAA TCCCCGCAAG CCACTCTCAC GCTGTTCCTC CAATGTGGGT 
GCCTCTGTCT CATCTGGTCG GCCAGGGCAC GACGATCTCG CCGAAGAGCT GCCCGACTTT 
GAGACGATTC TCATACGCAA GGCGCAGGAA TACAAAGAGA AAGAGTTGGC CATGATTGCC 
AATCCACATC TTGCCACGGA TCAGCCCAAA GATGCCATCA AGACACAGGT GGACGAAGGC 
TGGCTGCAGA GGAACACTAT GGAAAACACT CTTGACGCTG ATATGCACAG CCCAGAGCTA 
GAGCCCAATA ACAATGCCGA TCATTTGCCC ACGAAAGTCA ACTACGGCCT GGCGAACATC 
GATCTGAGCA TGCTGAGGCA AAGCAGGAGT TTGGAAGAGC ACCAGCAGCA GCCTGAAGTG 
AAGGCAGAGC CAAAGCAAGT GGCAGACACG GACGTGGTGG CGTTACCATC TGTTGAGCAG 
GAAGCGGACA GCGATTCCGA CTCTGTTGTG GCCGAAAGTG ACGAGGAGCC GGAACAGCAG 
GAGTATCGAC AATTAGCCAA GCGACGAAAG GTTGTTCCTG AGGTAGAGCC AGAGGTACAC 
GAAGCAGAAG CAGAAGCAAG CTCGGAACCG ATGGAAATGT CCTTACCGGA CCCCGAAGAA 
GAGACCATGC TCAATGACTT CTCTGCGGAT GATGAAGAAC AAGATGCCAC CTACGAGCCG 
GACGGAGTAA AGAAAGGCAA CGTAAAGCGT AAGCAAGCAG CTGGCAAAAG ACAAACCACC 
CAGAAGCCCA AAGCAGAAAA GAAGCCAAAG GCAGAGAAGC AGCTAAAAGC AGAGAAGAAG 
CCAAAAGCAG AGAAGAAGCC GAAGGCAGAG AAAAAGCCAA TGGCTGAGAA GAAGACCAAG 
TCAAAGAAAG CTCCTCCAGC TGCAGAGTCA GTGCCCGAAC AAGAGGAAGA AGAACAGAGC 
CAGCCCCTGA ATCCAGAAGA TCTCAAATAT ATGCTTGCCC TGGAGGCGGG CGATGTAACC 
TCCGTGCCAC GCATCAATCC CCGGGAACTG GAACTGGTAG ACGAAACAGC CCAGCGCTAT 
ATAAGTACCT TTGCCACGCC CACAATGGCC GGAGGCGGAG GCAACTTCCC AGTGAATGAG 
AAGCGAGCTG CAGCCAGAAA AAAGCTAGAG GAGCGTGTGG CCTCGGGCAA ACTGAACGAG 
AACTTTGTGA CCATCAACAT ACAGAAAAAG AAATTTGTGA GAGGTAAAAA ATCGGTGAAT 
TTTACCAAAT ACAAGAAGCA GCAATGGAGG CACAAGAAGC ACATAGCAGC ATTGACTGGC 
CCCGACATGG ACATGGGTGG CTGTGATGGT GGAGTGCTCA CCTGCTTCCA GTGCGGTGGC 
GTCGGACACT TTGCCCAGCA GTGCAAGATG AAGGGCGACA GTCTTCTACC GCTTTCCGCT 
CAGTTGGAAG AGGATCCCTC GCCGTTTCCC ACGCTGGCCG AGGCCCAGGA AATGGCCTCG 
CAGGGCGCCC TGGTGGCCCA CAGTCGCAAC ATTGAGCGAC TGCCCCACGC GGCCAATGCG 
GCCATTCTAC AAATAGACGA GATGGAGGAC TCGGATCAGA GTGAGGAGAG CCACAGCGAT 
GGGGAGAGGC AGCCTCGACC AGAGCCAGAC TGGTCGAGCG ATGATCCGGA TATTGATTTT 
GAGGCCTTAG ATGCTGCTGT GGAAGCTTCG CAGGCTGAGG CTCCGATCTC ACCCATTAAG 
ACCTACGTGG GGCACAAGAT ACCCGAAGAG TTCCTCAAAC AGGCTGGCCT GGATGGCGCC 
TCCACTTCGA CGACTGGGGG CTCCAGTCGA AGCAAGCATG GCGGAGTGCA GCCACTGTAT 
GATCTTCTGC CCGACGGCAG TGTTCAGGCT GCAACACCAG AGGTACTGGA GGCCCTACAC 
ATGTTTGGTC ACAGCAACTT TCGTAAAGGT CAGGATCGTG CCGTTATGCG CACACTCTCT 
GGACTCTCCT CTTTGGTCAC CCTAAGCACT GGCAGTGGCA AGTCGTTGTG CTACCAATTG 
CCCGCGTATC TGTACAATCG GCAGAGGGGT GCCATAACGC TGGTCATCTC GCCGCTGGTG 
TCGCTGATGG AGGATCAGGT GACGGGGGTG CCGCAGTTTC TTCGCGCGCA CTGCCTGCAC 
ACCAACCAGA CGCCCCAGCA GCGCATCAAG GTGCAGGAGA TGATTGCCAA CGGGGAGATT 
GATATTCTCC TGGTGTCGCC CGAGGCCGTT GTGTCGGGGG AGCGTGCCAC AGGGTTTGGG 
GCCATTCTGC GTAAGCTGCC GCCCATTGCC TTTGCGTGCA TCGACGAGGC ACATTGCGTG 
TCCCAGTGGA GCCATAATTT TCGACCCAGC TACCTGATGA TCTGCAAGGT CTTGAAGAAG 
AATCTTGGCG TGCAGACAGT GTTGGGTCTC ACAGCGACGG CCACGCTGCC CACGAGGGTG 
AGCATCATCA ATCACTTGGG CATCACGGAT GGGGAGCGGG GCATCATTAG TGACACTCCC 
TTGCCAGATA ATCTGGTCCT GTCCGTGTCC AAGGATGAAA ATCGCGATGC GGCACTGCTG 
CAACTGCTCA ACAGTGAGAG ATTCGAGTCC TGCCAGTCGA TCATCATCTA CTGCACGCGT 
CGTGATGAGT GTGAGCGGAT TGCCGGTTTC ATACGCACCT GTGTGCAGGA TCGCAGGAAA 
CCAGATGGAG CCCAGGAACA GGGGAAGAAG CGCAAGCGTG TGAACTGGCA GGCCGAGCCG 
TACCATGCGG GAATGCCCGC CTCGCGGCGC CGCACTATCC AGAAGGCCTT TATGGGGAAT 
GAGCTGCGCA TTGTGGTGGC CACCATTGCC TTTGGCATGG GCATCAATAA GCCGGACATT 
CGGGCTGTTA TCCACTACAA TATGCCGCGC AATTTCGAAA GTTATGTGCA GGAGATCGGA 
CGCGCTGGTC GCGATGGCTT GCATGCCCAC TGCCATCTCT TTCTGGACGC GAGGGGCGGC 
GACCAGGGCG AGCTGCGGCG TCATGTGTAC GCCAATTCCA TTGATCGCCA TGTGATAAGG 
AAGCTGCTGC AAAAGATCTT TGTACCCTGC AGCTGTGAGA AGCAATTGCC GAAAGGAACA 
GAACCGCAAG CCGCAGCTAT AGAAGCAGAC GGAACACGGT ACCACATCTG TCCTGGGCAT 
GAGATTGGCT TCTCTGTGGA GAAGACCGTT GAAGCTCTGG ACATTCCCGC CGAGAATATA 
TCAACGCTGC TCTGCTACAT GGAACTTGAG CCGCGCTGGT GCATAAATGT CCTCAGCTCA 
GCGTATATCA TGGCCAAGGT GATCTCCTAC GGCGGCCCAA AGTATTTAAA GCATGCTGCC 
AAAGAGTGTC CGCCACTGGC CATGGCCATT GCCCTGCAGA TTAAGGGCAA GACCTTCAAG 
GATGACTCTA ATATCATCGA GTTTTCGGTG ACGGATATTG CCGCCGGCAT TGGGTGGAAC 
AGCGGTGTGG TCAAGTACCA GCTGAAGGAC CTGGAATGGA TAAAAGTTGG TGGATATCCA 
AAGCGTTCGC CCATCACTGT CAGTTTCTAT GATTTGGGCT TCCGCATTAA GGTGCCCGGT 
GACTTCACCG AAACGGAGAT TGATAATGCA CTGGATACGC TCTATACGCG CTCCGTCAAG 
CAGGAGAAGA CACAGCTGAT TCAGCTGCAG TACGTAGCCC ATGGCCTGGC TGCCGTAGCC 
TACAGTTCGT GTCACCACTG CTGTAGTGCG GACTTTCCCC AGGACCGATG CACACAACTG 
AAGGCCATTG TGAGGAATTA CTTCCTCAAC GACTATCCGC AGGATCTTGA ACTGGAAATC 
GAGCCCAGCA ATGTGCCGGA TGAAAGCATC GTTGATGATG TTCATTCCCT GATCAACATG 
TACCCCGACA ACACATTCAC TGGACGAAAC ATTGCCCGCA TATTCCATGG CATCACGAGT 
CCAAATTACC CGGCCGTCAT GTGGGGACGT TGCAAGTTCT GGCGCGCCCA CATGAAAGTC 
GACTTCAATC GTATTCTCCA GTTGGCCAAT CAAGAGATTA TCAAACGGCG GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20388 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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