Dpse\GA20662 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20662
Entrez Gene ID	4813019
Full Name	GA20662 gene product from transcript GA20662-RB
Synonyms	GA20662,dpse_GLEANR_12389
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001353598.3	XP_001353634.1	uncharacterized protein, isoform A
XM_015187500.1	XP_015042986.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20662 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20662 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe08172	XM_001353598.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20662), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODe26322	XM_015187500.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20662), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20662 ( XM_001353598.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001353634.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20662), mRNA.

Price & Availability↑

CloneID	ODe08172
Clone ID Related Accession (Same CDS sequence)	XM_001353598.3
Accession Version	XM_001353598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001353598.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAAGA ATGGACACAG TAATGCGGGC TTTGTCAGCG ACCATGCGGA CACCGTGCAA 
AAGCCGATGG GGAAGACCTA CTCCCTGGAG CTGGCCGAGA AGGGTGGCAG CAACAACAGT 
AGCAGCAGCA AGAACAAGGC CATAGTGGCC AAGGATCCCG ACTACAATCC CTACCAGCAT 
CGCGATGTGG AGCATCCCAC AACCAATTCG GAAACACTGT TCCATCTGCT GAAGGGCTCT 
CTAGGAACGG GCATTCTGGC CATGCCGAAT GCCTTCCGCA ATTCTGGCTA CATTACAGGC 
TCCATTGGCA CCATTGTGAT TGGGTTTATA TGCACGTACT GCATCCATCA GCTGGTCAAA 
GCGGAGTTCG AGCTGTGCCG AAGGAAGAAG ATTCCTAGCA TGAACTATCC GGCCGTGGCG 
GAGACTGCTT TGGGCGAGGG ACCGGGTTTC TTTAGGGCCT GTGCCCCGTA CATTGGAACC 
GTAGTGAACA CCTTCCTGCT GATCTACCAG CTTGGCACCT GCTGTGTCTA CGTGGTTTTT 
GTCGCCTCAA ACATCAAGGC CATTGTGGAC GCCGTGGGGG ACACGAACAT CGATGTGCGC 
CTCTGTATGA TCATCATCTT GCTGCCGCTG ATCCTCATCA ATTGGGTGCG CAACCTCAAG 
TATTTGGCCC CATTCTCCAC GTTGGCCAAT GCCATTACCA TGGTCTCCTT TGGCATCATC 
TGCTACTACA TCTTCCGTGA GCCCGTTTCC ACCGAGGGCA AAGATGCGTT CGGCAAGCCC 
GAGAACTTTC CGCTCTTCTT CGGCACCGTT CTGTTCGCCT TGGAGGCCAT CGGTGTTATC 
CTACCGCTGG AGAATGAGAT GAAGACACCG CAAAAGTTTG GTGGAAACTG TGGTGTCCTC 
AACGTCTCAA TGGTCCTCAT TGTGTTCCTC TACGTGGGCA TGGGTCTCTT TGGCTATCTC 
AACTACGGCA ACGCCGTTCT GGGTTCCATT ACGCTGAATA TGCCGGAGCA TGAACTCCTG 
TCGCAGTGCG TGAAGGGTAT GCTGGCATTT GCCATCTACA TAACCCATGG ACTCGCCTGC 
TATGTGGCCA TTGACATCAC CTGGAACGAC TACGTGGGCA AGCGGTTGGG TCCCCAGCGC 
AACAAGCTCC TCTGGGAGTA CGCAGTCCGT ACGATTCTTG TCCTGATGAC TTTCCTTCTG 
GCTGTGGCCA TACCCAATCT GGAGCTGTTC ATCTCTCTTT TTGGTGCTTT GTGCCTGTCG 
GCTTTGGGTC TGGCCTTCCC GGCGCTCATC CAGATCTGCA CGCACTGGTA CGAGACCAAG 
GGGCTCCGCA AAGCCTGGCT TTTGTTGAGT AACTTTGTGC TCATCATTGT GGGCATCCTG 
GGGCTTGTCA TCGGCACTTA CACCTCACTC AAGGAGATCG TCCTGACCTT CTCAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001353634.1
CDS	430..1869
Translation	MSKNGHSNAGFVSDHADTVQKPMGKTYSLELAEKGGSNNSSSSKNKAIVAKDPDYNPYQHRDVEHPTTNSETLFHLLKGSLGTGILAMPNAFRNSGYITGSIGTIVIGFICTYCIHQLVKAEFELCRRKKIPSMNYPAVAETALGEGPGFFRACAPYIGTVVNTFLLIYQLGTCCVYVVFVASNIKAIVDAVGDTNIDVRLCMIIILLPLILINWVRNLKYLAPFSTLANAITMVSFGIICYYIFREPVSTEGKDAFGKPENFPLFFGTVLFALEAIGVILPLENEMKTPQKFGGNCGVLNVSMVLIVFLYVGMGLFGYLNYGNAVLGSITLNMPEHELLSQCVKGMLAFAIYITHGLACYVAIDITWNDYVGKRLGPQRNKLLWEYAVRTILVLMTFLLAVAIPNLELFISLFGALCLSALGLAFPALIQICTHWYETKGLRKAWLLLSNFVLIIVGILGLVIGTYTSLKEIVLTFSE

Target ORF information:

RefSeq Version	XM_001353598.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20662), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001353598.3

ORF Insert Sequence:

ATGAGTAAGA ATGGACACAG TAATGCGGGC TTTGTCAGCG ACCATGCGGA CACCGTGCAA 
AAGCCGATGG GGAAGACCTA CTCCCTGGAG CTGGCCGAGA AGGGTGGCAG CAACAACAGT 
AGCAGCAGCA AGAACAAGGC CATAGTGGCC AAGGATCCCG ACTACAATCC CTACCAGCAT 
CGCGATGTGG AGCATCCCAC AACCAATTCG GAAACACTGT TCCATCTGCT GAAGGGCTCT 
CTAGGAACGG GCATTCTGGC CATGCCGAAT GCCTTCCGCA ATTCTGGCTA CATTACAGGC 
TCCATTGGCA CCATTGTGAT TGGGTTTATA TGCACGTACT GCATCCATCA GCTGGTCAAA 
GCGGAGTTCG AGCTGTGCCG AAGGAAGAAG ATTCCTAGCA TGAACTATCC GGCCGTGGCG 
GAGACTGCTT TGGGCGAGGG ACCGGGTTTC TTTAGGGCCT GTGCCCCGTA CATTGGAACC 
GTAGTGAACA CCTTCCTGCT GATCTACCAG CTTGGCACCT GCTGTGTCTA CGTGGTTTTT 
GTCGCCTCAA ACATCAAGGC CATTGTGGAC GCCGTGGGGG ACACGAACAT CGATGTGCGC 
CTCTGTATGA TCATCATCTT GCTGCCGCTG ATCCTCATCA ATTGGGTGCG CAACCTCAAG 
TATTTGGCCC CATTCTCCAC GTTGGCCAAT GCCATTACCA TGGTCTCCTT TGGCATCATC 
TGCTACTACA TCTTCCGTGA GCCCGTTTCC ACCGAGGGCA AAGATGCGTT CGGCAAGCCC 
GAGAACTTTC CGCTCTTCTT CGGCACCGTT CTGTTCGCCT TGGAGGCCAT CGGTGTTATC 
CTACCGCTGG AGAATGAGAT GAAGACACCG CAAAAGTTTG GTGGAAACTG TGGTGTCCTC 
AACGTCTCAA TGGTCCTCAT TGTGTTCCTC TACGTGGGCA TGGGTCTCTT TGGCTATCTC 
AACTACGGCA ACGCCGTTCT GGGTTCCATT ACGCTGAATA TGCCGGAGCA TGAACTCCTG 
TCGCAGTGCG TGAAGGGTAT GCTGGCATTT GCCATCTACA TAACCCATGG ACTCGCCTGC 
TATGTGGCCA TTGACATCAC CTGGAACGAC TACGTGGGCA AGCGGTTGGG TCCCCAGCGC 
AACAAGCTCC TCTGGGAGTA CGCAGTCCGT ACGATTCTTG TCCTGATGAC TTTCCTTCTG 
GCTGTGGCCA TACCCAATCT GGAGCTGTTC ATCTCTCTTT TTGGTGCTTT GTGCCTGTCG 
GCTTTGGGTC TGGCCTTCCC GGCGCTCATC CAGATCTGCA CGCACTGGTA CGAGACCAAG 
GGGCTCCGCA AAGCCTGGCT TTTGTTGAGT AACTTTGTGC TCATCATTGT GGGCATCCTG 
GGGCTTGTCA TCGGCACTTA CACCTCACTC AAGGAGATCG TCCTGACCTT CTCAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA20662 ( XM_015187500.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042986.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20662), mRNA.

Price & Availability↑

CloneID	ODe26322
Clone ID Related Accession (Same CDS sequence)	XM_015187500.1
Accession Version	XM_015187500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGTG TCAGCCCCCC CAACGATGAT ACCTTTAAAC CAACAGCCAT GAGTAAGAAT 
GGACACAGTA ATGCGGGCTT TGTCAGCGAC CATGCGGACA CCGTGCAAAA GCCGATGGGG 
AAGACCTACT CCCTGGAGCT GGCCGAGAAG GGTGGCAGCA ACAACAGTAG CAGCAGCAAG 
AACAAGGCCA TAGTGGCCAA GGATCCCGAC TACAATCCCT ACCAGCATCG CGATGTGGAG 
CATCCCACAA CCAATTCGGA AACACTGTTC CATCTGCTGA AGGGCTCTCT AGGAACGGGC 
ATTCTGGCCA TGCCGAATGC CTTCCGCAAT TCTGGCTACA TTACAGGCTC CATTGGCACC 
ATTGTGATTG GGTTTATATG CACGTACTGC ATCCATCAGC TGGTCAAAGC GGAGTTCGAG 
CTGTGCCGAA GGAAGAAGAT TCCTAGCATG AACTATCCGG CCGTGGCGGA GACTGCTTTG 
GGCGAGGGAC CGGGTTTCTT TAGGGCCTGT GCCCCGTACA TTGGAACCGT AGTGAACACC 
TTCCTGCTGA TCTACCAGCT TGGCACCTGC TGTGTCTACG TGGTTTTTGT CGCCTCAAAC 
ATCAAGGCCA TTGTGGACGC CGTGGGGGAC ACGAACATCG ATGTGCGCCT CTGTATGATC 
ATCATCTTGC TGCCGCTGAT CCTCATCAAT TGGGTGCGCA ACCTCAAGTA TTTGGCCCCA 
TTCTCCACGT TGGCCAATGC CATTACCATG GTCTCCTTTG GCATCATCTG CTACTACATC 
TTCCGTGAGC CCGTTTCCAC CGAGGGCAAA GATGCGTTCG GCAAGCCCGA GAACTTTCCG 
CTCTTCTTCG GCACCGTTCT GTTCGCCTTG GAGGCCATCG GTGTTATCCT ACCGCTGGAG 
AATGAGATGA AGACACCGCA AAAGTTTGGT GGAAACTGTG GTGTCCTCAA CGTCTCAATG 
GTCCTCATTG TGTTCCTCTA CGTGGGCATG GGTCTCTTTG GCTATCTCAA CTACGGCAAC 
GCCGTTCTGG GTTCCATTAC GCTGAATATG CCGGAGCATG AACTCCTGTC GCAGTGCGTG 
AAGGGTATGC TGGCATTTGC CATCTACATA ACCCATGGAC TCGCCTGCTA TGTGGCCATT 
GACATCACCT GGAACGACTA CGTGGGCAAG CGGTTGGGTC CCCAGCGCAA CAAGCTCCTC 
TGGGAGTACG CAGTCCGTAC GATTCTTGTC CTGATGACTT TCCTTCTGGC TGTGGCCATA 
CCCAATCTGG AGCTGTTCAT CTCTCTTTTT GGTGCTTTGT GCCTGTCGGC TTTGGGTCTG 
GCCTTCCCGG CGCTCATCCA GATCTGCACG CACTGGTACG AGACCAAGGG GCTCCGCAAA 
GCCTGGCTTT TGTTGAGTAA CTTTGTGCTC ATCATTGTGG GCATCCTGGG GCTTGTCATC 
GGCACTTACA CCTCACTCAA GGAGATCGTC CTGACCTTCT CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042986.1
CDS	292..1779
Translation	MKCVSPPNDDTFKPTAMSKNGHSNAGFVSDHADTVQKPMGKTYSLELAEKGGSNNSSSSKNKAIVAKDPDYNPYQHRDVEHPTTNSETLFHLLKGSLGTGILAMPNAFRNSGYITGSIGTIVIGFICTYCIHQLVKAEFELCRRKKIPSMNYPAVAETALGEGPGFFRACAPYIGTVVNTFLLIYQLGTCCVYVVFVASNIKAIVDAVGDTNIDVRLCMIIILLPLILINWVRNLKYLAPFSTLANAITMVSFGIICYYIFREPVSTEGKDAFGKPENFPLFFGTVLFALEAIGVILPLENEMKTPQKFGGNCGVLNVSMVLIVFLYVGMGLFGYLNYGNAVLGSITLNMPEHELLSQCVKGMLAFAIYITHGLACYVAIDITWNDYVGKRLGPQRNKLLWEYAVRTILVLMTFLLAVAIPNLELFISLFGALCLSALGLAFPALIQICTHWYETKGLRKAWLLLSNFVLIIVGILGLVIGTYTSLKEIVLTFSE

Target ORF information:

RefSeq Version	XM_015187500.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20662), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187500.1

ORF Insert Sequence:

ATGAAGTGTG TCAGCCCCCC CAACGATGAT ACCTTTAAAC CAACAGCCAT GAGTAAGAAT 
GGACACAGTA ATGCGGGCTT TGTCAGCGAC CATGCGGACA CCGTGCAAAA GCCGATGGGG 
AAGACCTACT CCCTGGAGCT GGCCGAGAAG GGTGGCAGCA ACAACAGTAG CAGCAGCAAG 
AACAAGGCCA TAGTGGCCAA GGATCCCGAC TACAATCCCT ACCAGCATCG CGATGTGGAG 
CATCCCACAA CCAATTCGGA AACACTGTTC CATCTGCTGA AGGGCTCTCT AGGAACGGGC 
ATTCTGGCCA TGCCGAATGC CTTCCGCAAT TCTGGCTACA TTACAGGCTC CATTGGCACC 
ATTGTGATTG GGTTTATATG CACGTACTGC ATCCATCAGC TGGTCAAAGC GGAGTTCGAG 
CTGTGCCGAA GGAAGAAGAT TCCTAGCATG AACTATCCGG CCGTGGCGGA GACTGCTTTG 
GGCGAGGGAC CGGGTTTCTT TAGGGCCTGT GCCCCGTACA TTGGAACCGT AGTGAACACC 
TTCCTGCTGA TCTACCAGCT TGGCACCTGC TGTGTCTACG TGGTTTTTGT CGCCTCAAAC 
ATCAAGGCCA TTGTGGACGC CGTGGGGGAC ACGAACATCG ATGTGCGCCT CTGTATGATC 
ATCATCTTGC TGCCGCTGAT CCTCATCAAT TGGGTGCGCA ACCTCAAGTA TTTGGCCCCA 
TTCTCCACGT TGGCCAATGC CATTACCATG GTCTCCTTTG GCATCATCTG CTACTACATC 
TTCCGTGAGC CCGTTTCCAC CGAGGGCAAA GATGCGTTCG GCAAGCCCGA GAACTTTCCG 
CTCTTCTTCG GCACCGTTCT GTTCGCCTTG GAGGCCATCG GTGTTATCCT ACCGCTGGAG 
AATGAGATGA AGACACCGCA AAAGTTTGGT GGAAACTGTG GTGTCCTCAA CGTCTCAATG 
GTCCTCATTG TGTTCCTCTA CGTGGGCATG GGTCTCTTTG GCTATCTCAA CTACGGCAAC 
GCCGTTCTGG GTTCCATTAC GCTGAATATG CCGGAGCATG AACTCCTGTC GCAGTGCGTG 
AAGGGTATGC TGGCATTTGC CATCTACATA ACCCATGGAC TCGCCTGCTA TGTGGCCATT 
GACATCACCT GGAACGACTA CGTGGGCAAG CGGTTGGGTC CCCAGCGCAA CAAGCTCCTC 
TGGGAGTACG CAGTCCGTAC GATTCTTGTC CTGATGACTT TCCTTCTGGC TGTGGCCATA 
CCCAATCTGG AGCTGTTCAT CTCTCTTTTT GGTGCTTTGT GCCTGTCGGC TTTGGGTCTG 
GCCTTCCCGG CGCTCATCCA GATCTGCACG CACTGGTACG AGACCAAGGG GCTCCGCAAA 
GCCTGGCTTT TGTTGAGTAA CTTTGTGCTC ATCATTGTGG GCATCCTGGG GCTTGTCATC 
GGCACTTACA CCTCACTCAA GGAGATCGTC CTGACCTTCT CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20662 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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