Dpse\GA13959 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA13959
Entrez Gene ID	4803569
Full Name	GA13959 gene product from transcript GA13959-RE
Synonyms	GA13959,dpse_GLEANR_14853
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015183700.1	XP_015039186.1	uncharacterized protein, isoform B
XM_015183701.1	XP_015039187.1	uncharacterized protein, isoform C
XM_015183702.1	XP_015039188.1	uncharacterized protein, isoform D
XM_001360239.3	XP_001360276.2	uncharacterized protein, isoform A
XM_015183703.1	XP_015039189.1	uncharacterized protein, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA13959 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA13959 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe22393	XM_015183701.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe22394	XM_015183702.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA13959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe02917	XM_001360239.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe02917	XM_015183700.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe02917	XM_015183703.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA13959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA13959 ( XM_015183701.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039187.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13959), mRNA.

Price & Availability↑

CloneID	ODe22393
Clone ID Related Accession (Same CDS sequence)	XM_015183701.1
Accession Version	XM_015183701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TGGTGAACTG 
GAGCAACAAC TGCTGCAGGC GAATCCCATA CTGGAGGCCT TTGGCAATGC CAAGACCGTC 
AAGAACGATA ATTCATCGCG TTTCGGCAAA TTCATACGGA TCAATTTCGA TGCCTCTGGT 
TTCATCTCGG GCGCCAACAT CGAGACGTAT CTGCTGGAGA AGTCGCGGGC CATCCGTCAA 
GCCAAGGACG AACGTACATT CCACATCTTC TATCAGTTGC TGGCGGGCGC CTCGCCGGAG 
CAGCGCGAGA AGTTCATACT CGATGATGTC AAGTCGTATG CATTCCTGTC GAACGGCAGC 
CTGCCAGTGC CCGGTGTGGA TGACTATGCC GAGTTCCAGG CCACCGTCAA GTCGATGAAC 
ATCATGGGCA TGACCTCGGA GGACTTCAAC TCGATATTCC GTATCGTGAG CGCCGTCCTG 
CTGTTCGGAA GCATGAAGTT CCGGCAGGAG CGCAACAACG ACCAGGCCAC GCTACCCGAC 
AACACAGTGG CCCAGAAGAT TGCCCATTTG CTCGGCCTGA GTGTGACGGA CATGTCGCGG 
GCCTTCCTCA CGCCGCGCAT CAAGGTGGGC CGCGACTTTG TCACCAAGGC CCAGACCAAG 
GAGCAGGTGG AGTTCGCTGT GGAGGCCATT GCCAAGGCCT GCTACGAGCG GATGTTCAAG 
TGGCTGGTGA ATCGCATCAA TCGGTCGCTG GACCGCACCA AGCGGCAGGG GGCCTCCTTC 
ATCGGCATCC TCGACATGGC TGGCTTCGAG ATTTTCGAGC TCAACTCGTT CGAGCAGCTG 
TGCATCAACT ACACGAACGA GAAGCTGCAG CAGCTCTTCA ACCACACCAT GTTCATCCTG 
GAGCAGGAGG AGTACCAGCG CGAGGGCATC GAGTGGAAGT TCATTGATTT TGGGCTCGAT 
CTGCAGCCCA CCATTGACCT GATCGACAAA CCGGGCGGCA TCATGGCCCT GCTCGACGAG 
GAGTGCTGGT TCCCCAAGGC CACCGACAAG ACGTTTGTCG ACAAGCTCGT CTCGGCCCAC 
TCGATGCATC CGAAGTTCAT GAAGACCGAC TTCCGGGGCG TGGCCGATTT TGCCATCGTC 
CACTATGCCG GACGGGTGGA CTACTCGGCG ACCAAGTGGC TGATGAAGAA CATGGATCCG 
CTGAACGAGA ACATTGTGTC GCTGCTGCAG GGCTCGCAGG ATCCGTTTGT GGTGAACATC 
TGGAAGGATG CGGAGATCGT TGGCATGGCA CAGCAGGCCC TGACCGACAC CCAGTTCGGG 
GCACGCACCC GCAAGGGCAT GTTCCGCACC GTGTCCCACC TATACAAGGA GCAGCTGGCG 
AAGCTGATGG ACACGCTGCG CAACACGAAT CCCAATTTTG TGCGCTGTAT CATACCGAAC 
CACGAGAAGC GGGCCGGCAA GATCGATGCC CCCCTGGTGC TCGACCAGTT GCGTTGCAAC 
GGCGTCCTCG AGGGCATACG CATCTGCCGC CAAGGCTTCC CCAATCGCAT ACCCTTCCAG 
GAGTTCCGCC AGCGGTACGA GCTGCTCACT CCGAACGTCA TCGCCAAGGG CTTCATGGAC 
GGCAAGAAGG CCTGCGAGAG GATGATCCAG GCGCTGGAAC TCGACTCGAA TCTGTATCGA 
GTCGGTCAGT CGAAGATCTT CTTCCGCGCG GGCGTCCTCG CCCATCTCGA GGAGGAGCGT 
GACTTCAAGA TCTCCGACCT AATCGTGAAC TTCCAGGCGT TCTGTCGCGG CTTCCTCGCC 
CGCCGCAACT ACCAGAAGCG GCTCCAGCAG CTGAATGCCA TCCGGATCAT TCAGCGCAAC 
TGCGCCGCCT ACCTCAAGCT CCGCAACTGG CAGTGGTGGC GCCTCTACAC GAAGGTCAAG 
CCCCTGCTGG AGGTCACCAA GCAGGAGGAG AAGCTCGTCC AGAAGGAGGA TGAGCTGAAG 
CAGGTGCGCG AGAAGCTCGA CACGCTGGCC AAGAACACCC AGGAGTACGA GCGCAAGTAC 
CAGCAGGCGC TTGTGGAGAA AACCACGCTC GCCGAACAGC TGCAGGCCGA GATTGAGCTG 
TGCGCCGAGG CGGAGGAGTC GCGCTCGCGT CTGATGGCTC GTAAACAGGA GCTGGAGGAC 
ATGATGCAGG AGCTGGAGAC GCGCGTCGAG GAGGAGGAGG AGCGTGTGTT GGCCCTGGGC 
GGTGAGAAGA AAAAGCTGGA GCTGCACATC CAGGACCTCG AGGAGCAGCT GGAAGAGGAA 
GAGGCTGCGC GCCAGAAGCT TCAGCTAGAG AAGGTCCAGT TGGACGGGAA GATCAAGAAG 
CACGAGGAGG ATCTCGCCCT CACCGACGAC CAGAACCAAA AGCTGCTCAA GGAGAAGAAG 
CTGCTCGAAG AGCGGGCCAA CGATCTCTCC CAGACGCTCG CCGAGGAGGA GGAGAAGGCC 
AAGCACCTGG CCAAACTGAA GGCCAAGCAC GAGGCCACCA TCGCCGAGCT CGAGGAGCGC 
ATGCACAAGG ATCAGCAGCA GCGCCAGGAG ACGGACCGCA CCAAGCGAAA GGTCGAAACC 
GAGGTGGCGG ACCTTAGGGA GCAGCTGAAC GAGCGACGCA CCCAAGTGGA GGAGATGCAG 
GCGCAGCTGT TGAAGCGCGA GGAGGAGCTC ACCCAGACCC TGATGCGCAT TGACGAGGAG 
TCCGCCACCA AGGCCACCGC TCAGAAGGCC CAGCGTGAAC TCGAATCGCA GCTCGCCGAG 
ATCCAGGAGG ATCTCGAGGC CGAGAAGGCG GCCCGCGCCA AGGCCGAGAA GGTGCGACGC 
GACCTCAGCG AGGAGCTCGA AGCACTCAAG AACGAGCTTC TCGACTCCCT GGACACCACG 
GCCGCCCAAC AAGAGCTGCG CTCCAAGCGC GAACAGGAGC TGGCCACGCT GAAGAAGTCG 
CTGGAGGAAG AGGGTGTGAA CCACGAGGGC GTCCTGGCCG ACATGCGGCA CAAGCACTCG 
CAGGAGCTGA ACGGCATCAA CGATCAGTTG GAGAACCTGC GCAAAGCCAA GTCCGTGCTA 
GAGAAGGCCA AGGGTACGCT GGAGGCGGAG AACGCCGATC TGGCCACCGA ATTGCGCAGC 
GTCAACAGCT CCCGGCAGGA GAACGATCGA CGACGCAAGC AGGCCGAATC CCAAATCGCA 
GAGCTTCAGG TGAAATTGGC CGAGATCGAA CGCGCACGAT CGGAATTGCA GGAGAAGTGC 
ACAAAGCTGC AGCAGGAGGC GGAGAATATC ACCAATCAGC TGGAGGAGGC CGAGCTGAAG 
GCATCGGCAG CCGTCAAGTC GGCCAGCAAC ATGGAGTCGC AGCTGACGGA AGCGCAGCAG 
CAGCTGGAGG AGGAGACACG CCAGAAACTG GGCCTCAGCT CCAAGCTGCG CCAGATCGAG 
TCCGAGAAGG AGGCGCTGCA GGAGCAGCTC GAGGAGGATG AGGAGGCCAA GCGTAACTTT 
GAGCGCAAGC TGGCCGAGGT CACCGGCCAG ATGCAGGAGA TTAAGAAGAA GGCTGAGGAG 
GATGCCGACC TGGCCAAGGA GCTGGAGGAG GGCAAGAAGC GGCTCAACAA GGAGATCGAG 
GTGTTGGAGC GACAGGTGAA GGAGCTGATT GCCCAGAACG ATCGCCTGGA CAAGAGCAAA 
AAGAAGATCC AATCGGAGCT GGAGGATGCC ACCATCGAGC TGGAGACGCA ACGCACGAAG 
GTGCTCGAGT TGGAGAAGAA GCAGAAGAAC TTCGACAAGA CCCTCGCCGA GGAGAAAGCC 
ATATCAGAGC AGATCGCCCA GGAGCGCGAT ACTGCCGAGC GGGAGGCGCG CGAGAAGGAG 
ACCAAGGTGC TGTCGGTGTC CCGCGAGCTG GACGAGGCCT TCGACAAGAT CGAAGATCTC 
GAGAACAAGC GCAAGACACT CCAGAACGAG TTAGACGACC TGGCCAACTC CCAAGGCACC 
GCCGACAAGA ACGTCCACGA GCTTGAGAAG GCCAAGCGCG CCCTGGAGTC GCAGCTTGCC 
GAGCTGAAAG CACAAAACGA GGAGCTCGAG GACGACCTCC AGCTGACGGA GGATGCCAAG 
CTGCGTCTCG AGGTCAATAT GCAGGCCCTG CGCTCCCAGT TCGAGCGCGA CCTGTTGGCC 
AAGGAGGAGG GGGCAGAGGA GAAGCGGCGA GGACTGGTCA AGCAATTGCG CGATCTGGAA 
GCGGAGCTCG ATGAGGAGCG CAAGCAGCGA ACGGCCGCCG TGGCATCGAA GAAGAAGCTG 
GAGGGCGACC TCAAGGAGAT CGAGACCACG ATGGAGATGC ATAACAAGGT GAAGGAGGAT 
GCCCTGAAGC ACGCCAAGAA GCTGCAAGCA CAAGTCAAGG ATGCGCTTCG TGACGCCGAG 
GAGGCCAAGG CGGCCAAGGA GGAGCTGCAG GCGCAGAGCA AGGAGGCCGA GCGCAAGGTC 
AAGGCCTTGG AGGCAGAAGT ATTACAGCTG ACCGAGGATT TGGCCAGCTC GGAGCGGGCC 
CGACGGGCGG CCGAAACGGA GCGCGACGAG CTTGCCGAGG AGATTGCCAA CAGTTCCAGC 
AAGGGATCGC TGATGGTCGA CGAGAAGCGG CGACTGGAGG CCCGCATTGC CACACTCGAG 
GAGGAGCTGG AGGAGGAGCA GTCCAATTCG GAGGTTCTGC TCGACCGCAG CCGCAAGGCG 
CAGCTGCAGA TCGAGCAGCT GACCACCGAG CTAGCCAACG AGAAGTCCAA CTCGCAGAAG 
AACGAGAATG GACGCGCCCT GCTCGAGCGC CAGAACAAGG AGCTGAAGGC CAAGCTGGCC 
GAGATTGAGA CGGCACAACG TACCAAGGTG AAGGCCACCA TCGCCACGCT CGAGGCCAAA 
ATCGCCAATC TGGAGGAGCA GCTGGAGAAC GAGGGCAAGG AGCGACTGCT GCAGCAGAAG 
GCCAACCGCA AGATGGACAA GAAGATCAAG GAGCTGACGA TGAACATCGA AGACGAGCGG 
AGACATGTCG ACCAGCACAA GGAGCAAATG GATAAGCTGA ATAGCCGCAT CAAATTGCTC 
AAGCGCAATC TGGACGAGAC GGAGGAGGAA CTGCAGAAGG AGAAGACACA GAAGCGCAAG 
TACCAGCGCG AGTGCGAGGA TATGATTGAA TCGCAGGAGG CCATGAACCG TGAGATCAAC 
TCACTCAAAA CTAAACTACG ACGCACCGGC GGCATGGGCC TCAGCTCCAG CCGGCTCACG 
GGCACACCTT CATCGAAGCG CGGAGGAGGC GGTGGTGGGG GCGGCGGCGG CGGTGACGAC 
AGCTCATCGG TGCAGGATGA ATCATTAGAT GGCGAGGATT CGGGCAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039187.1
CDS	430..6360
Translation	MSEEVDRNDPELKYLSVERNQYNDPATQAEWTQKRLVWVPHENQGFVAASIKREHGDEVEVELAETGKRVMILRDDIQKMNPPKFDKVEDMAELTCLNEASVLHNIKDRYYSGLIYTYSGLFCVVVNPYKKLPIYTEKIMERYKGIKRHEVPPHVFAITDSAYRNMLGDREDQSILCTGESGAGKTENTKKVIQFLAYVAASKPKGSGAVPNPAILIGELEQQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDASGFISGANIETYLLEKSRAIRQAKDERTFHIFYQLLAGASPEQREKFILDDVKSYAFLSNGSLPVPGVDDYAEFQATVKSMNIMGMTSEDFNSIFRIVSAVLLFGSMKFRQERNNDQATLPDNTVAQKIAHLLGLSVTDMSRAFLTPRIKVGRDFVTKAQTKEQVEFAVEAIAKACYERMFKWLVNRINRSLDRTKRQGASFIGILDMAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWKFIDFGLDLQPTIDLIDKPGGIMALLDEECWFPKATDKTFVDKLVSAHSMHPKFMKTDFRGVADFAIVHYAGRVDYSATKWLMKNMDPLNENIVSLLQGSQDPFVVNIWKDAEIVGMAQQALTDTQFGARTRKGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIAKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDFKISDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLRNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLDTLAKNTQEYERKYQQALVEKTTLAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELETRVEEEEERVLALGGEKKKLELHIQDLEEQLEEEEAARQKLQLEKVQLDGKIKKHEEDLALTDDQNQKLLKEKKLLEERANDLSQTLAEEEEKAKHLAKLKAKHEATIAELEERMHKDQQQRQETDRTKRKVETEVADLREQLNERRTQVEEMQAQLLKREEELTQTLMRIDEESATKATAQKAQRELESQLAEIQEDLEAEKAARAKAEKVRRDLSEELEALKNELLDSLDTTAAQQELRSKREQELATLKKSLEEEGVNHEGVLADMRHKHSQELNGINDQLENLRKAKSVLEKAKGTLEAENADLATELRSVNSSRQENDRRRKQAESQIAELQVKLAEIERARSELQEKCTKLQQEAENITNQLEEAELKASAAVKSASNMESQLTEAQQQLEEETRQKLGLSSKLRQIESEKEALQEQLEEDEEAKRNFERKLAEVTGQMQEIKKKAEEDADLAKELEEGKKRLNKEIEVLERQVKELIAQNDRLDKSKKKIQSELEDATIELETQRTKVLELEKKQKNFDKTLAEEKAISEQIAQERDTAEREAREKETKVLSVSRELDEAFDKIEDLENKRKTLQNELDDLANSQGTADKNVHELEKAKRALESQLAELKAQNEELEDDLQLTEDAKLRLEVNMQALRSQFERDLLAKEEGAEEKRRGLVKQLRDLEAELDEERKQRTAAVASKKKLEGDLKEIETTMEMHNKVKEDALKHAKKLQAQVKDALRDAEEAKAAKEELQAQSKEAERKVKALEAEVLQLTEDLASSERARRAAETERDELAEEIANSSSKGSLMVDEKRRLEARIATLEEELEEEQSNSEVLLDRSRKAQLQIEQLTTELANEKSNSQKNENGRALLERQNKELKAKLAEIETAQRTKVKATIATLEAKIANLEEQLENEGKERLLQQKANRKMDKKIKELTMNIEDERRHVDQHKEQMDKLNSRIKLLKRNLDETEEELQKEKTQKRKYQRECEDMIESQEAMNREINSLKTKLRRTGGMGLSSSRLTGTPSSKRGGGGGGGGGGGDDSSSVQDESLDGEDSGN

Target ORF information:

RefSeq Version	XM_015183701.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183701.1

ORF Insert Sequence:

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TGGTGAACTG 
GAGCAACAAC TGCTGCAGGC GAATCCCATA CTGGAGGCCT TTGGCAATGC CAAGACCGTC 
AAGAACGATA ATTCATCGCG TTTCGGCAAA TTCATACGGA TCAATTTCGA TGCCTCTGGT 
TTCATCTCGG GCGCCAACAT CGAGACGTAT CTGCTGGAGA AGTCGCGGGC CATCCGTCAA 
GCCAAGGACG AACGTACATT CCACATCTTC TATCAGTTGC TGGCGGGCGC CTCGCCGGAG 
CAGCGCGAGA AGTTCATACT CGATGATGTC AAGTCGTATG CATTCCTGTC GAACGGCAGC 
CTGCCAGTGC CCGGTGTGGA TGACTATGCC GAGTTCCAGG CCACCGTCAA GTCGATGAAC 
ATCATGGGCA TGACCTCGGA GGACTTCAAC TCGATATTCC GTATCGTGAG CGCCGTCCTG 
CTGTTCGGAA GCATGAAGTT CCGGCAGGAG CGCAACAACG ACCAGGCCAC GCTACCCGAC 
AACACAGTGG CCCAGAAGAT TGCCCATTTG CTCGGCCTGA GTGTGACGGA CATGTCGCGG 
GCCTTCCTCA CGCCGCGCAT CAAGGTGGGC CGCGACTTTG TCACCAAGGC CCAGACCAAG 
GAGCAGGTGG AGTTCGCTGT GGAGGCCATT GCCAAGGCCT GCTACGAGCG GATGTTCAAG 
TGGCTGGTGA ATCGCATCAA TCGGTCGCTG GACCGCACCA AGCGGCAGGG GGCCTCCTTC 
ATCGGCATCC TCGACATGGC TGGCTTCGAG ATTTTCGAGC TCAACTCGTT CGAGCAGCTG 
TGCATCAACT ACACGAACGA GAAGCTGCAG CAGCTCTTCA ACCACACCAT GTTCATCCTG 
GAGCAGGAGG AGTACCAGCG CGAGGGCATC GAGTGGAAGT TCATTGATTT TGGGCTCGAT 
CTGCAGCCCA CCATTGACCT GATCGACAAA CCGGGCGGCA TCATGGCCCT GCTCGACGAG 
GAGTGCTGGT TCCCCAAGGC CACCGACAAG ACGTTTGTCG ACAAGCTCGT CTCGGCCCAC 
TCGATGCATC CGAAGTTCAT GAAGACCGAC TTCCGGGGCG TGGCCGATTT TGCCATCGTC 
CACTATGCCG GACGGGTGGA CTACTCGGCG ACCAAGTGGC TGATGAAGAA CATGGATCCG 
CTGAACGAGA ACATTGTGTC GCTGCTGCAG GGCTCGCAGG ATCCGTTTGT GGTGAACATC 
TGGAAGGATG CGGAGATCGT TGGCATGGCA CAGCAGGCCC TGACCGACAC CCAGTTCGGG 
GCACGCACCC GCAAGGGCAT GTTCCGCACC GTGTCCCACC TATACAAGGA GCAGCTGGCG 
AAGCTGATGG ACACGCTGCG CAACACGAAT CCCAATTTTG TGCGCTGTAT CATACCGAAC 
CACGAGAAGC GGGCCGGCAA GATCGATGCC CCCCTGGTGC TCGACCAGTT GCGTTGCAAC 
GGCGTCCTCG AGGGCATACG CATCTGCCGC CAAGGCTTCC CCAATCGCAT ACCCTTCCAG 
GAGTTCCGCC AGCGGTACGA GCTGCTCACT CCGAACGTCA TCGCCAAGGG CTTCATGGAC 
GGCAAGAAGG CCTGCGAGAG GATGATCCAG GCGCTGGAAC TCGACTCGAA TCTGTATCGA 
GTCGGTCAGT CGAAGATCTT CTTCCGCGCG GGCGTCCTCG CCCATCTCGA GGAGGAGCGT 
GACTTCAAGA TCTCCGACCT AATCGTGAAC TTCCAGGCGT TCTGTCGCGG CTTCCTCGCC 
CGCCGCAACT ACCAGAAGCG GCTCCAGCAG CTGAATGCCA TCCGGATCAT TCAGCGCAAC 
TGCGCCGCCT ACCTCAAGCT CCGCAACTGG CAGTGGTGGC GCCTCTACAC GAAGGTCAAG 
CCCCTGCTGG AGGTCACCAA GCAGGAGGAG AAGCTCGTCC AGAAGGAGGA TGAGCTGAAG 
CAGGTGCGCG AGAAGCTCGA CACGCTGGCC AAGAACACCC AGGAGTACGA GCGCAAGTAC 
CAGCAGGCGC TTGTGGAGAA AACCACGCTC GCCGAACAGC TGCAGGCCGA GATTGAGCTG 
TGCGCCGAGG CGGAGGAGTC GCGCTCGCGT CTGATGGCTC GTAAACAGGA GCTGGAGGAC 
ATGATGCAGG AGCTGGAGAC GCGCGTCGAG GAGGAGGAGG AGCGTGTGTT GGCCCTGGGC 
GGTGAGAAGA AAAAGCTGGA GCTGCACATC CAGGACCTCG AGGAGCAGCT GGAAGAGGAA 
GAGGCTGCGC GCCAGAAGCT TCAGCTAGAG AAGGTCCAGT TGGACGGGAA GATCAAGAAG 
CACGAGGAGG ATCTCGCCCT CACCGACGAC CAGAACCAAA AGCTGCTCAA GGAGAAGAAG 
CTGCTCGAAG AGCGGGCCAA CGATCTCTCC CAGACGCTCG CCGAGGAGGA GGAGAAGGCC 
AAGCACCTGG CCAAACTGAA GGCCAAGCAC GAGGCCACCA TCGCCGAGCT CGAGGAGCGC 
ATGCACAAGG ATCAGCAGCA GCGCCAGGAG ACGGACCGCA CCAAGCGAAA GGTCGAAACC 
GAGGTGGCGG ACCTTAGGGA GCAGCTGAAC GAGCGACGCA CCCAAGTGGA GGAGATGCAG 
GCGCAGCTGT TGAAGCGCGA GGAGGAGCTC ACCCAGACCC TGATGCGCAT TGACGAGGAG 
TCCGCCACCA AGGCCACCGC TCAGAAGGCC CAGCGTGAAC TCGAATCGCA GCTCGCCGAG 
ATCCAGGAGG ATCTCGAGGC CGAGAAGGCG GCCCGCGCCA AGGCCGAGAA GGTGCGACGC 
GACCTCAGCG AGGAGCTCGA AGCACTCAAG AACGAGCTTC TCGACTCCCT GGACACCACG 
GCCGCCCAAC AAGAGCTGCG CTCCAAGCGC GAACAGGAGC TGGCCACGCT GAAGAAGTCG 
CTGGAGGAAG AGGGTGTGAA CCACGAGGGC GTCCTGGCCG ACATGCGGCA CAAGCACTCG 
CAGGAGCTGA ACGGCATCAA CGATCAGTTG GAGAACCTGC GCAAAGCCAA GTCCGTGCTA 
GAGAAGGCCA AGGGTACGCT GGAGGCGGAG AACGCCGATC TGGCCACCGA ATTGCGCAGC 
GTCAACAGCT CCCGGCAGGA GAACGATCGA CGACGCAAGC AGGCCGAATC CCAAATCGCA 
GAGCTTCAGG TGAAATTGGC CGAGATCGAA CGCGCACGAT CGGAATTGCA GGAGAAGTGC 
ACAAAGCTGC AGCAGGAGGC GGAGAATATC ACCAATCAGC TGGAGGAGGC CGAGCTGAAG 
GCATCGGCAG CCGTCAAGTC GGCCAGCAAC ATGGAGTCGC AGCTGACGGA AGCGCAGCAG 
CAGCTGGAGG AGGAGACACG CCAGAAACTG GGCCTCAGCT CCAAGCTGCG CCAGATCGAG 
TCCGAGAAGG AGGCGCTGCA GGAGCAGCTC GAGGAGGATG AGGAGGCCAA GCGTAACTTT 
GAGCGCAAGC TGGCCGAGGT CACCGGCCAG ATGCAGGAGA TTAAGAAGAA GGCTGAGGAG 
GATGCCGACC TGGCCAAGGA GCTGGAGGAG GGCAAGAAGC GGCTCAACAA GGAGATCGAG 
GTGTTGGAGC GACAGGTGAA GGAGCTGATT GCCCAGAACG ATCGCCTGGA CAAGAGCAAA 
AAGAAGATCC AATCGGAGCT GGAGGATGCC ACCATCGAGC TGGAGACGCA ACGCACGAAG 
GTGCTCGAGT TGGAGAAGAA GCAGAAGAAC TTCGACAAGA CCCTCGCCGA GGAGAAAGCC 
ATATCAGAGC AGATCGCCCA GGAGCGCGAT ACTGCCGAGC GGGAGGCGCG CGAGAAGGAG 
ACCAAGGTGC TGTCGGTGTC CCGCGAGCTG GACGAGGCCT TCGACAAGAT CGAAGATCTC 
GAGAACAAGC GCAAGACACT CCAGAACGAG TTAGACGACC TGGCCAACTC CCAAGGCACC 
GCCGACAAGA ACGTCCACGA GCTTGAGAAG GCCAAGCGCG CCCTGGAGTC GCAGCTTGCC 
GAGCTGAAAG CACAAAACGA GGAGCTCGAG GACGACCTCC AGCTGACGGA GGATGCCAAG 
CTGCGTCTCG AGGTCAATAT GCAGGCCCTG CGCTCCCAGT TCGAGCGCGA CCTGTTGGCC 
AAGGAGGAGG GGGCAGAGGA GAAGCGGCGA GGACTGGTCA AGCAATTGCG CGATCTGGAA 
GCGGAGCTCG ATGAGGAGCG CAAGCAGCGA ACGGCCGCCG TGGCATCGAA GAAGAAGCTG 
GAGGGCGACC TCAAGGAGAT CGAGACCACG ATGGAGATGC ATAACAAGGT GAAGGAGGAT 
GCCCTGAAGC ACGCCAAGAA GCTGCAAGCA CAAGTCAAGG ATGCGCTTCG TGACGCCGAG 
GAGGCCAAGG CGGCCAAGGA GGAGCTGCAG GCGCAGAGCA AGGAGGCCGA GCGCAAGGTC 
AAGGCCTTGG AGGCAGAAGT ATTACAGCTG ACCGAGGATT TGGCCAGCTC GGAGCGGGCC 
CGACGGGCGG CCGAAACGGA GCGCGACGAG CTTGCCGAGG AGATTGCCAA CAGTTCCAGC 
AAGGGATCGC TGATGGTCGA CGAGAAGCGG CGACTGGAGG CCCGCATTGC CACACTCGAG 
GAGGAGCTGG AGGAGGAGCA GTCCAATTCG GAGGTTCTGC TCGACCGCAG CCGCAAGGCG 
CAGCTGCAGA TCGAGCAGCT GACCACCGAG CTAGCCAACG AGAAGTCCAA CTCGCAGAAG 
AACGAGAATG GACGCGCCCT GCTCGAGCGC CAGAACAAGG AGCTGAAGGC CAAGCTGGCC 
GAGATTGAGA CGGCACAACG TACCAAGGTG AAGGCCACCA TCGCCACGCT CGAGGCCAAA 
ATCGCCAATC TGGAGGAGCA GCTGGAGAAC GAGGGCAAGG AGCGACTGCT GCAGCAGAAG 
GCCAACCGCA AGATGGACAA GAAGATCAAG GAGCTGACGA TGAACATCGA AGACGAGCGG 
AGACATGTCG ACCAGCACAA GGAGCAAATG GATAAGCTGA ATAGCCGCAT CAAATTGCTC 
AAGCGCAATC TGGACGAGAC GGAGGAGGAA CTGCAGAAGG AGAAGACACA GAAGCGCAAG 
TACCAGCGCG AGTGCGAGGA TATGATTGAA TCGCAGGAGG CCATGAACCG TGAGATCAAC 
TCACTCAAAA CTAAACTACG ACGCACCGGC GGCATGGGCC TCAGCTCCAG CCGGCTCACG 
GGCACACCTT CATCGAAGCG CGGAGGAGGC GGTGGTGGGG GCGGCGGCGG CGGTGACGAC 
AGCTCATCGG TGCAGGATGA ATCATTAGAT GGCGAGGATT CGGGCAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13959 ( XM_015183702.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039188.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA13959), mRNA.

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CloneID	ODe22394
Clone ID Related Accession (Same CDS sequence)	XM_015183702.1
Accession Version	XM_015183702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGCTCCAT CCAAGTGGCC GAGACCGAAA AGGCCAAGGC CTATTCGCCC 
AAAAAGGATG CCGCACTCAT TATGAATTGT GAGGGTCTGC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039188.1
CDS	431..6415
Translation	MSEEVDRNDPELKYLSVERNQYNDPATQAEWTQKRLVWVPHENQGFVAASIKREHGDEVEVELAETGKRVMILRDDIQKMNPPKFDKVEDMAELTCLNEASVLHNIKDRYYSGLIYTYSGLFCVVVNPYKKLPIYTEKIMERYKGIKRHEVPPHVFAITDSAYRNMLGDREDQSILCTGESGAGKTENTKKVIQFLAYVAASKPKGSGAVPNPAILINFSVLTNKYLTVKIVTQNQKQYKYQRNIDVAMEIDENEGELEQQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDASGFISGANIETYLLEKSRAIRQAKDERTFHIFYQLLAGASPEQREKFILDDVKSYAFLSNGSLPVPGVDDYAEFQATVKSMNIMGMTSEDFNSIFRIVSAVLLFGSMKFRQERNNDQATLPDNTVAQKIAHLLGLSVTDMSRAFLTPRIKVGRDFVTKAQTKEQVEFAVEAIAKACYERMFKWLVNRINRSLDRTKRQGASFIGILDMAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWKFIDFGLDLQPTIDLIDKPGGIMALLDEECWFPKATDKTFVDKLVSAHSMHPKFMKTDFRGVADFAIVHYAGRVDYSATKWLMKNMDPLNENIVSLLQGSQDPFVVNIWKDAEIVGMAQQALTDTQFGARTRKGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIAKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDFKISDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLRNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLDTLAKNTQEYERKYQQALVEKTTLAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELETRVEEEEERVLALGGEKKKLELHIQDLEEQLEEEEAARQKLQLEKVQLDGKIKKHEEDLALTDDQNQKLLKEKKLLEERANDLSQTLAEEEEKAKHLAKLKAKHEATIAELEERMHKDQQQRQETDRTKRKVETEVADLREQLNERRTQVEEMQAQLLKREEELTQTLMRIDEESATKATAQKAQRELESQLAEIQEDLEAEKAARAKAEKVRRDLSEELEALKNELLDSLDTTAAQQELRSKREQELATLKKSLEEEGVNHEGVLADMRHKHSQELNGINDQLENLRKAKSVLEKAKGTLEAENADLATELRSVNSSRQENDRRRKQAESQIAELQVKLAEIERARSELQEKCTKLQQEAENITNQLEEAELKASAAVKSASNMESQLTEAQQQLEEETRQKLGLSSKLRQIESEKEALQEQLEEDEEAKRNFERKLAEVTGQMQEIKKKAEEDADLAKELEEGKKRLNKEIEVLERQVKELIAQNDRLDKSKKKIQSELEDATIELETQRTKVLELEKKQKNFDKTLAEEKAISEQIAQERDTAEREAREKETKVLSVSRELDEAFDKIEDLENKRKTLQNELDDLANSQGTADKNVHELEKAKRALESQLAELKAQNEELEDDLQLTEDAKLRLEVNMQALRSQFERDLLAKEEGAEEKRRGLVKQLRDLEAELDEERKQRTAAVASKKKLEGDLKEIETTMEMHNKVKEDALKHAKKLQAQVKDALRDAEEAKAAKEELQAQSKEAERKVKALEAEVLQLTEDLASSERARRAAETERDELAEEIANSSSKGSLMVDEKRRLEARIATLEEELEEEQSNSEVLLDRSRKAQLQIEQLTTELANEKSNSQKNENGRALLERQNKELKAKLAEIETAQRTKVKATIATLEAKIANLEEQLENEGKERLLQQKANRKMDKKIKELTMNIEDERRHVDQHKEQMDKLNSRIKLLKRNLDETEEELQKEKTQKRKYQRECEDMIESQEAMNREINSLKTKLRSIQVAETEKAKAYSPKKDAALIMNCEGLH

Target ORF information:

RefSeq Version	XM_015183702.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA13959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183702.1

ORF Insert Sequence:

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGCTCCAT CCAAGTGGCC GAGACCGAAA AGGCCAAGGC CTATTCGCCC 
AAAAAGGATG CCGCACTCAT TATGAATTGT GAGGGTCTGC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13959 ( XM_015183700.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039186.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13959), mRNA.

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CloneID	ODe02917
Clone ID Related Accession (Same CDS sequence)	XM_015183700.1 , XM_001360239.3 , XM_015183703.1
Accession Version	XM_015183700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039186.1
CDS	431..6475
Translation	MSEEVDRNDPELKYLSVERNQYNDPATQAEWTQKRLVWVPHENQGFVAASIKREHGDEVEVELAETGKRVMILRDDIQKMNPPKFDKVEDMAELTCLNEASVLHNIKDRYYSGLIYTYSGLFCVVVNPYKKLPIYTEKIMERYKGIKRHEVPPHVFAITDSAYRNMLGDREDQSILCTGESGAGKTENTKKVIQFLAYVAASKPKGSGAVPNPAILINFSVLTNKYLTVKIVTQNQKQYKYQRNIDVAMEIDENEGELEQQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDASGFISGANIETYLLEKSRAIRQAKDERTFHIFYQLLAGASPEQREKFILDDVKSYAFLSNGSLPVPGVDDYAEFQATVKSMNIMGMTSEDFNSIFRIVSAVLLFGSMKFRQERNNDQATLPDNTVAQKIAHLLGLSVTDMSRAFLTPRIKVGRDFVTKAQTKEQVEFAVEAIAKACYERMFKWLVNRINRSLDRTKRQGASFIGILDMAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWKFIDFGLDLQPTIDLIDKPGGIMALLDEECWFPKATDKTFVDKLVSAHSMHPKFMKTDFRGVADFAIVHYAGRVDYSATKWLMKNMDPLNENIVSLLQGSQDPFVVNIWKDAEIVGMAQQALTDTQFGARTRKGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIAKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDFKISDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLRNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLDTLAKNTQEYERKYQQALVEKTTLAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELETRVEEEEERVLALGGEKKKLELHIQDLEEQLEEEEAARQKLQLEKVQLDGKIKKHEEDLALTDDQNQKLLKEKKLLEERANDLSQTLAEEEEKAKHLAKLKAKHEATIAELEERMHKDQQQRQETDRTKRKVETEVADLREQLNERRTQVEEMQAQLLKREEELTQTLMRIDEESATKATAQKAQRELESQLAEIQEDLEAEKAARAKAEKVRRDLSEELEALKNELLDSLDTTAAQQELRSKREQELATLKKSLEEEGVNHEGVLADMRHKHSQELNGINDQLENLRKAKSVLEKAKGTLEAENADLATELRSVNSSRQENDRRRKQAESQIAELQVKLAEIERARSELQEKCTKLQQEAENITNQLEEAELKASAAVKSASNMESQLTEAQQQLEEETRQKLGLSSKLRQIESEKEALQEQLEEDEEAKRNFERKLAEVTGQMQEIKKKAEEDADLAKELEEGKKRLNKEIEVLERQVKELIAQNDRLDKSKKKIQSELEDATIELETQRTKVLELEKKQKNFDKTLAEEKAISEQIAQERDTAEREAREKETKVLSVSRELDEAFDKIEDLENKRKTLQNELDDLANSQGTADKNVHELEKAKRALESQLAELKAQNEELEDDLQLTEDAKLRLEVNMQALRSQFERDLLAKEEGAEEKRRGLVKQLRDLEAELDEERKQRTAAVASKKKLEGDLKEIETTMEMHNKVKEDALKHAKKLQAQVKDALRDAEEAKAAKEELQAQSKEAERKVKALEAEVLQLTEDLASSERARRAAETERDELAEEIANSSSKGSLMVDEKRRLEARIATLEEELEEEQSNSEVLLDRSRKAQLQIEQLTTELANEKSNSQKNENGRALLERQNKELKAKLAEIETAQRTKVKATIATLEAKIANLEEQLENEGKERLLQQKANRKMDKKIKELTMNIEDERRHVDQHKEQMDKLNSRIKLLKRNLDETEEELQKEKTQKRKYQRECEDMIESQEAMNREINSLKTKLRRTGGMGLSSSRLTGTPSSKRGGGGGGGGGGGDDSSSVQDESLDGEDSGN

Target ORF information:

RefSeq Version	XM_015183700.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183700.1

ORF Insert Sequence:

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13959 ( XM_001360239.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360276.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13959), mRNA.

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CloneID	ODe02917
Clone ID Related Accession (Same CDS sequence)	XM_015183700.1 , XM_001360239.3 , XM_015183703.1
Accession Version	XM_001360239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360239.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360276.2
CDS	245..6289
Translation	MSEEVDRNDPELKYLSVERNQYNDPATQAEWTQKRLVWVPHENQGFVAASIKREHGDEVEVELAETGKRVMILRDDIQKMNPPKFDKVEDMAELTCLNEASVLHNIKDRYYSGLIYTYSGLFCVVVNPYKKLPIYTEKIMERYKGIKRHEVPPHVFAITDSAYRNMLGDREDQSILCTGESGAGKTENTKKVIQFLAYVAASKPKGSGAVPNPAILINFSVLTNKYLTVKIVTQNQKQYKYQRNIDVAMEIDENEGELEQQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDASGFISGANIETYLLEKSRAIRQAKDERTFHIFYQLLAGASPEQREKFILDDVKSYAFLSNGSLPVPGVDDYAEFQATVKSMNIMGMTSEDFNSIFRIVSAVLLFGSMKFRQERNNDQATLPDNTVAQKIAHLLGLSVTDMSRAFLTPRIKVGRDFVTKAQTKEQVEFAVEAIAKACYERMFKWLVNRINRSLDRTKRQGASFIGILDMAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWKFIDFGLDLQPTIDLIDKPGGIMALLDEECWFPKATDKTFVDKLVSAHSMHPKFMKTDFRGVADFAIVHYAGRVDYSATKWLMKNMDPLNENIVSLLQGSQDPFVVNIWKDAEIVGMAQQALTDTQFGARTRKGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIAKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDFKISDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLRNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLDTLAKNTQEYERKYQQALVEKTTLAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELETRVEEEEERVLALGGEKKKLELHIQDLEEQLEEEEAARQKLQLEKVQLDGKIKKHEEDLALTDDQNQKLLKEKKLLEERANDLSQTLAEEEEKAKHLAKLKAKHEATIAELEERMHKDQQQRQETDRTKRKVETEVADLREQLNERRTQVEEMQAQLLKREEELTQTLMRIDEESATKATAQKAQRELESQLAEIQEDLEAEKAARAKAEKVRRDLSEELEALKNELLDSLDTTAAQQELRSKREQELATLKKSLEEEGVNHEGVLADMRHKHSQELNGINDQLENLRKAKSVLEKAKGTLEAENADLATELRSVNSSRQENDRRRKQAESQIAELQVKLAEIERARSELQEKCTKLQQEAENITNQLEEAELKASAAVKSASNMESQLTEAQQQLEEETRQKLGLSSKLRQIESEKEALQEQLEEDEEAKRNFERKLAEVTGQMQEIKKKAEEDADLAKELEEGKKRLNKEIEVLERQVKELIAQNDRLDKSKKKIQSELEDATIELETQRTKVLELEKKQKNFDKTLAEEKAISEQIAQERDTAEREAREKETKVLSVSRELDEAFDKIEDLENKRKTLQNELDDLANSQGTADKNVHELEKAKRALESQLAELKAQNEELEDDLQLTEDAKLRLEVNMQALRSQFERDLLAKEEGAEEKRRGLVKQLRDLEAELDEERKQRTAAVASKKKLEGDLKEIETTMEMHNKVKEDALKHAKKLQAQVKDALRDAEEAKAAKEELQAQSKEAERKVKALEAEVLQLTEDLASSERARRAAETERDELAEEIANSSSKGSLMVDEKRRLEARIATLEEELEEEQSNSEVLLDRSRKAQLQIEQLTTELANEKSNSQKNENGRALLERQNKELKAKLAEIETAQRTKVKATIATLEAKIANLEEQLENEGKERLLQQKANRKMDKKIKELTMNIEDERRHVDQHKEQMDKLNSRIKLLKRNLDETEEELQKEKTQKRKYQRECEDMIESQEAMNREINSLKTKLRRTGGMGLSSSRLTGTPSSKRGGGGGGGGGGGDDSSSVQDESLDGEDSGN

Target ORF information:

RefSeq Version	XM_001360239.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA13959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360239.3

ORF Insert Sequence:

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13959 ( XM_015183703.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039189.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA13959), mRNA.

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CloneID	ODe02917
Clone ID Related Accession (Same CDS sequence)	XM_015183700.1 , XM_001360239.3 , XM_015183703.1
Accession Version	XM_015183703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039189.1
CDS	388..6432
Translation	MSEEVDRNDPELKYLSVERNQYNDPATQAEWTQKRLVWVPHENQGFVAASIKREHGDEVEVELAETGKRVMILRDDIQKMNPPKFDKVEDMAELTCLNEASVLHNIKDRYYSGLIYTYSGLFCVVVNPYKKLPIYTEKIMERYKGIKRHEVPPHVFAITDSAYRNMLGDREDQSILCTGESGAGKTENTKKVIQFLAYVAASKPKGSGAVPNPAILINFSVLTNKYLTVKIVTQNQKQYKYQRNIDVAMEIDENEGELEQQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDASGFISGANIETYLLEKSRAIRQAKDERTFHIFYQLLAGASPEQREKFILDDVKSYAFLSNGSLPVPGVDDYAEFQATVKSMNIMGMTSEDFNSIFRIVSAVLLFGSMKFRQERNNDQATLPDNTVAQKIAHLLGLSVTDMSRAFLTPRIKVGRDFVTKAQTKEQVEFAVEAIAKACYERMFKWLVNRINRSLDRTKRQGASFIGILDMAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWKFIDFGLDLQPTIDLIDKPGGIMALLDEECWFPKATDKTFVDKLVSAHSMHPKFMKTDFRGVADFAIVHYAGRVDYSATKWLMKNMDPLNENIVSLLQGSQDPFVVNIWKDAEIVGMAQQALTDTQFGARTRKGMFRTVSHLYKEQLAKLMDTLRNTNPNFVRCIIPNHEKRAGKIDAPLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNVIAKGFMDGKKACERMIQALELDSNLYRVGQSKIFFRAGVLAHLEEERDFKISDLIVNFQAFCRGFLARRNYQKRLQQLNAIRIIQRNCAAYLKLRNWQWWRLYTKVKPLLEVTKQEEKLVQKEDELKQVREKLDTLAKNTQEYERKYQQALVEKTTLAEQLQAEIELCAEAEESRSRLMARKQELEDMMQELETRVEEEEERVLALGGEKKKLELHIQDLEEQLEEEEAARQKLQLEKVQLDGKIKKHEEDLALTDDQNQKLLKEKKLLEERANDLSQTLAEEEEKAKHLAKLKAKHEATIAELEERMHKDQQQRQETDRTKRKVETEVADLREQLNERRTQVEEMQAQLLKREEELTQTLMRIDEESATKATAQKAQRELESQLAEIQEDLEAEKAARAKAEKVRRDLSEELEALKNELLDSLDTTAAQQELRSKREQELATLKKSLEEEGVNHEGVLADMRHKHSQELNGINDQLENLRKAKSVLEKAKGTLEAENADLATELRSVNSSRQENDRRRKQAESQIAELQVKLAEIERARSELQEKCTKLQQEAENITNQLEEAELKASAAVKSASNMESQLTEAQQQLEEETRQKLGLSSKLRQIESEKEALQEQLEEDEEAKRNFERKLAEVTGQMQEIKKKAEEDADLAKELEEGKKRLNKEIEVLERQVKELIAQNDRLDKSKKKIQSELEDATIELETQRTKVLELEKKQKNFDKTLAEEKAISEQIAQERDTAEREAREKETKVLSVSRELDEAFDKIEDLENKRKTLQNELDDLANSQGTADKNVHELEKAKRALESQLAELKAQNEELEDDLQLTEDAKLRLEVNMQALRSQFERDLLAKEEGAEEKRRGLVKQLRDLEAELDEERKQRTAAVASKKKLEGDLKEIETTMEMHNKVKEDALKHAKKLQAQVKDALRDAEEAKAAKEELQAQSKEAERKVKALEAEVLQLTEDLASSERARRAAETERDELAEEIANSSSKGSLMVDEKRRLEARIATLEEELEEEQSNSEVLLDRSRKAQLQIEQLTTELANEKSNSQKNENGRALLERQNKELKAKLAEIETAQRTKVKATIATLEAKIANLEEQLENEGKERLLQQKANRKMDKKIKELTMNIEDERRHVDQHKEQMDKLNSRIKLLKRNLDETEEELQKEKTQKRKYQRECEDMIESQEAMNREINSLKTKLRRTGGMGLSSSRLTGTPSSKRGGGGGGGGGGGDDSSSVQDESLDGEDSGN

Target ORF information:

RefSeq Version	XM_015183703.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA13959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183703.1

ORF Insert Sequence:

ATGTCGGAAG AAGTAGATCG CAACGATCCC GAGCTGAAAT ACCTTTCGGT GGAACGGAAC 
CAATACAATG ATCCGGCCAC ACAGGCGGAA TGGACACAGA AGCGCCTGGT CTGGGTGCCC 
CACGAGAATC AGGGCTTCGT GGCCGCCAGT ATTAAGCGGG AACATGGCGA CGAGGTTGAG 
GTTGAACTGG CCGAGACCGG CAAGAGGGTC ATGATCCTGC GCGACGACAT ACAGAAGATG 
AATCCGCCAA AATTCGACAA AGTCGAGGAT ATGGCGGAGC TGACGTGCCT CAACGAGGCC 
TCCGTGCTGC ACAACATTAA AGACAGATAC TACTCTGGCT TGATCTATAC ATATTCCGGT 
CTATTTTGCG TTGTGGTCAA TCCGTACAAG AAACTCCCCA TCTACACCGA AAAGATTATG 
GAACGGTATA AGGGTATAAA GCGACACGAA GTGCCACCGC ATGTGTTTGC AATAACGGAC 
AGTGCCTATA GAAACATGTT GGGTGATCGC GAAGACCAAT CGATTTTGTG TACTGGCGAA 
TCGGGTGCTG GCAAGACGGA GAACACCAAG AAGGTCATCC AATTTCTGGC CTATGTGGCA 
GCCTCCAAGC CCAAGGGCTC TGGTGCGGTG CCGAATCCTG CCATTCTGAT TAACTTTTCG 
GTGTTAACGA ATAAATACTT AACCGTCAAG ATAGTGACCC AGAATCAAAA ACAATATAAA 
TATCAACGGA ATATCGATGT AGCAATGGAG ATTGATGAGA ATGAGGGTGA ACTGGAGCAA 
CAACTGCTGC AGGCGAATCC CATACTGGAG GCCTTTGGCA ATGCCAAGAC CGTCAAGAAC 
GATAATTCAT CGCGTTTCGG CAAATTCATA CGGATCAATT TCGATGCCTC TGGTTTCATC 
TCGGGCGCCA ACATCGAGAC GTATCTGCTG GAGAAGTCGC GGGCCATCCG TCAAGCCAAG 
GACGAACGTA CATTCCACAT CTTCTATCAG TTGCTGGCGG GCGCCTCGCC GGAGCAGCGC 
GAGAAGTTCA TACTCGATGA TGTCAAGTCG TATGCATTCC TGTCGAACGG CAGCCTGCCA 
GTGCCCGGTG TGGATGACTA TGCCGAGTTC CAGGCCACCG TCAAGTCGAT GAACATCATG 
GGCATGACCT CGGAGGACTT CAACTCGATA TTCCGTATCG TGAGCGCCGT CCTGCTGTTC 
GGAAGCATGA AGTTCCGGCA GGAGCGCAAC AACGACCAGG CCACGCTACC CGACAACACA 
GTGGCCCAGA AGATTGCCCA TTTGCTCGGC CTGAGTGTGA CGGACATGTC GCGGGCCTTC 
CTCACGCCGC GCATCAAGGT GGGCCGCGAC TTTGTCACCA AGGCCCAGAC CAAGGAGCAG 
GTGGAGTTCG CTGTGGAGGC CATTGCCAAG GCCTGCTACG AGCGGATGTT CAAGTGGCTG 
GTGAATCGCA TCAATCGGTC GCTGGACCGC ACCAAGCGGC AGGGGGCCTC CTTCATCGGC 
ATCCTCGACA TGGCTGGCTT CGAGATTTTC GAGCTCAACT CGTTCGAGCA GCTGTGCATC 
AACTACACGA ACGAGAAGCT GCAGCAGCTC TTCAACCACA CCATGTTCAT CCTGGAGCAG 
GAGGAGTACC AGCGCGAGGG CATCGAGTGG AAGTTCATTG ATTTTGGGCT CGATCTGCAG 
CCCACCATTG ACCTGATCGA CAAACCGGGC GGCATCATGG CCCTGCTCGA CGAGGAGTGC 
TGGTTCCCCA AGGCCACCGA CAAGACGTTT GTCGACAAGC TCGTCTCGGC CCACTCGATG 
CATCCGAAGT TCATGAAGAC CGACTTCCGG GGCGTGGCCG ATTTTGCCAT CGTCCACTAT 
GCCGGACGGG TGGACTACTC GGCGACCAAG TGGCTGATGA AGAACATGGA TCCGCTGAAC 
GAGAACATTG TGTCGCTGCT GCAGGGCTCG CAGGATCCGT TTGTGGTGAA CATCTGGAAG 
GATGCGGAGA TCGTTGGCAT GGCACAGCAG GCCCTGACCG ACACCCAGTT CGGGGCACGC 
ACCCGCAAGG GCATGTTCCG CACCGTGTCC CACCTATACA AGGAGCAGCT GGCGAAGCTG 
ATGGACACGC TGCGCAACAC GAATCCCAAT TTTGTGCGCT GTATCATACC GAACCACGAG 
AAGCGGGCCG GCAAGATCGA TGCCCCCCTG GTGCTCGACC AGTTGCGTTG CAACGGCGTC 
CTCGAGGGCA TACGCATCTG CCGCCAAGGC TTCCCCAATC GCATACCCTT CCAGGAGTTC 
CGCCAGCGGT ACGAGCTGCT CACTCCGAAC GTCATCGCCA AGGGCTTCAT GGACGGCAAG 
AAGGCCTGCG AGAGGATGAT CCAGGCGCTG GAACTCGACT CGAATCTGTA TCGAGTCGGT 
CAGTCGAAGA TCTTCTTCCG CGCGGGCGTC CTCGCCCATC TCGAGGAGGA GCGTGACTTC 
AAGATCTCCG ACCTAATCGT GAACTTCCAG GCGTTCTGTC GCGGCTTCCT CGCCCGCCGC 
AACTACCAGA AGCGGCTCCA GCAGCTGAAT GCCATCCGGA TCATTCAGCG CAACTGCGCC 
GCCTACCTCA AGCTCCGCAA CTGGCAGTGG TGGCGCCTCT ACACGAAGGT CAAGCCCCTG 
CTGGAGGTCA CCAAGCAGGA GGAGAAGCTC GTCCAGAAGG AGGATGAGCT GAAGCAGGTG 
CGCGAGAAGC TCGACACGCT GGCCAAGAAC ACCCAGGAGT ACGAGCGCAA GTACCAGCAG 
GCGCTTGTGG AGAAAACCAC GCTCGCCGAA CAGCTGCAGG CCGAGATTGA GCTGTGCGCC 
GAGGCGGAGG AGTCGCGCTC GCGTCTGATG GCTCGTAAAC AGGAGCTGGA GGACATGATG 
CAGGAGCTGG AGACGCGCGT CGAGGAGGAG GAGGAGCGTG TGTTGGCCCT GGGCGGTGAG 
AAGAAAAAGC TGGAGCTGCA CATCCAGGAC CTCGAGGAGC AGCTGGAAGA GGAAGAGGCT 
GCGCGCCAGA AGCTTCAGCT AGAGAAGGTC CAGTTGGACG GGAAGATCAA GAAGCACGAG 
GAGGATCTCG CCCTCACCGA CGACCAGAAC CAAAAGCTGC TCAAGGAGAA GAAGCTGCTC 
GAAGAGCGGG CCAACGATCT CTCCCAGACG CTCGCCGAGG AGGAGGAGAA GGCCAAGCAC 
CTGGCCAAAC TGAAGGCCAA GCACGAGGCC ACCATCGCCG AGCTCGAGGA GCGCATGCAC 
AAGGATCAGC AGCAGCGCCA GGAGACGGAC CGCACCAAGC GAAAGGTCGA AACCGAGGTG 
GCGGACCTTA GGGAGCAGCT GAACGAGCGA CGCACCCAAG TGGAGGAGAT GCAGGCGCAG 
CTGTTGAAGC GCGAGGAGGA GCTCACCCAG ACCCTGATGC GCATTGACGA GGAGTCCGCC 
ACCAAGGCCA CCGCTCAGAA GGCCCAGCGT GAACTCGAAT CGCAGCTCGC CGAGATCCAG 
GAGGATCTCG AGGCCGAGAA GGCGGCCCGC GCCAAGGCCG AGAAGGTGCG ACGCGACCTC 
AGCGAGGAGC TCGAAGCACT CAAGAACGAG CTTCTCGACT CCCTGGACAC CACGGCCGCC 
CAACAAGAGC TGCGCTCCAA GCGCGAACAG GAGCTGGCCA CGCTGAAGAA GTCGCTGGAG 
GAAGAGGGTG TGAACCACGA GGGCGTCCTG GCCGACATGC GGCACAAGCA CTCGCAGGAG 
CTGAACGGCA TCAACGATCA GTTGGAGAAC CTGCGCAAAG CCAAGTCCGT GCTAGAGAAG 
GCCAAGGGTA CGCTGGAGGC GGAGAACGCC GATCTGGCCA CCGAATTGCG CAGCGTCAAC 
AGCTCCCGGC AGGAGAACGA TCGACGACGC AAGCAGGCCG AATCCCAAAT CGCAGAGCTT 
CAGGTGAAAT TGGCCGAGAT CGAACGCGCA CGATCGGAAT TGCAGGAGAA GTGCACAAAG 
CTGCAGCAGG AGGCGGAGAA TATCACCAAT CAGCTGGAGG AGGCCGAGCT GAAGGCATCG 
GCAGCCGTCA AGTCGGCCAG CAACATGGAG TCGCAGCTGA CGGAAGCGCA GCAGCAGCTG 
GAGGAGGAGA CACGCCAGAA ACTGGGCCTC AGCTCCAAGC TGCGCCAGAT CGAGTCCGAG 
AAGGAGGCGC TGCAGGAGCA GCTCGAGGAG GATGAGGAGG CCAAGCGTAA CTTTGAGCGC 
AAGCTGGCCG AGGTCACCGG CCAGATGCAG GAGATTAAGA AGAAGGCTGA GGAGGATGCC 
GACCTGGCCA AGGAGCTGGA GGAGGGCAAG AAGCGGCTCA ACAAGGAGAT CGAGGTGTTG 
GAGCGACAGG TGAAGGAGCT GATTGCCCAG AACGATCGCC TGGACAAGAG CAAAAAGAAG 
ATCCAATCGG AGCTGGAGGA TGCCACCATC GAGCTGGAGA CGCAACGCAC GAAGGTGCTC 
GAGTTGGAGA AGAAGCAGAA GAACTTCGAC AAGACCCTCG CCGAGGAGAA AGCCATATCA 
GAGCAGATCG CCCAGGAGCG CGATACTGCC GAGCGGGAGG CGCGCGAGAA GGAGACCAAG 
GTGCTGTCGG TGTCCCGCGA GCTGGACGAG GCCTTCGACA AGATCGAAGA TCTCGAGAAC 
AAGCGCAAGA CACTCCAGAA CGAGTTAGAC GACCTGGCCA ACTCCCAAGG CACCGCCGAC 
AAGAACGTCC ACGAGCTTGA GAAGGCCAAG CGCGCCCTGG AGTCGCAGCT TGCCGAGCTG 
AAAGCACAAA ACGAGGAGCT CGAGGACGAC CTCCAGCTGA CGGAGGATGC CAAGCTGCGT 
CTCGAGGTCA ATATGCAGGC CCTGCGCTCC CAGTTCGAGC GCGACCTGTT GGCCAAGGAG 
GAGGGGGCAG AGGAGAAGCG GCGAGGACTG GTCAAGCAAT TGCGCGATCT GGAAGCGGAG 
CTCGATGAGG AGCGCAAGCA GCGAACGGCC GCCGTGGCAT CGAAGAAGAA GCTGGAGGGC 
GACCTCAAGG AGATCGAGAC CACGATGGAG ATGCATAACA AGGTGAAGGA GGATGCCCTG 
AAGCACGCCA AGAAGCTGCA AGCACAAGTC AAGGATGCGC TTCGTGACGC CGAGGAGGCC 
AAGGCGGCCA AGGAGGAGCT GCAGGCGCAG AGCAAGGAGG CCGAGCGCAA GGTCAAGGCC 
TTGGAGGCAG AAGTATTACA GCTGACCGAG GATTTGGCCA GCTCGGAGCG GGCCCGACGG 
GCGGCCGAAA CGGAGCGCGA CGAGCTTGCC GAGGAGATTG CCAACAGTTC CAGCAAGGGA 
TCGCTGATGG TCGACGAGAA GCGGCGACTG GAGGCCCGCA TTGCCACACT CGAGGAGGAG 
CTGGAGGAGG AGCAGTCCAA TTCGGAGGTT CTGCTCGACC GCAGCCGCAA GGCGCAGCTG 
CAGATCGAGC AGCTGACCAC CGAGCTAGCC AACGAGAAGT CCAACTCGCA GAAGAACGAG 
AATGGACGCG CCCTGCTCGA GCGCCAGAAC AAGGAGCTGA AGGCCAAGCT GGCCGAGATT 
GAGACGGCAC AACGTACCAA GGTGAAGGCC ACCATCGCCA CGCTCGAGGC CAAAATCGCC 
AATCTGGAGG AGCAGCTGGA GAACGAGGGC AAGGAGCGAC TGCTGCAGCA GAAGGCCAAC 
CGCAAGATGG ACAAGAAGAT CAAGGAGCTG ACGATGAACA TCGAAGACGA GCGGAGACAT 
GTCGACCAGC ACAAGGAGCA AATGGATAAG CTGAATAGCC GCATCAAATT GCTCAAGCGC 
AATCTGGACG AGACGGAGGA GGAACTGCAG AAGGAGAAGA CACAGAAGCG CAAGTACCAG 
CGCGAGTGCG AGGATATGAT TGAATCGCAG GAGGCCATGA ACCGTGAGAT CAACTCACTC 
AAAACTAAAC TACGACGCAC CGGCGGCATG GGCCTCAGCT CCAGCCGGCT CACGGGCACA 
CCTTCATCGA AGCGCGGAGG AGGCGGTGGT GGGGGCGGCG GCGGCGGTGA CGACAGCTCA 
TCGGTGCAGG ATGAATCATT AGATGGCGAG GATTCGGGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA13959 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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