Spag5 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag5
Entrez Gene ID	101829006
Full Name	sperm associated antigen 5
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021231158.1	XP_021086817.1	sperm-associated antigen 5 isoform X2
XM_021231157.1	XP_021086816.1	sperm-associated antigen 5 isoform X1
XM_005077017.2	XP_005077074.1	sperm-associated antigen 5
XM_005077017.3	XP_005077074.1	sperm-associated antigen 5 isoform X4
XM_021231159.1	XP_021086818.1	sperm-associated antigen 5 isoform X3

Pathways from BioSystems
Gene Ontology

The following Spag5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe130621	XM_005077017.3 Latest version!	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMe251171	XM_021231157.1 Latest version!	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMe251172	XM_021231158.1 Latest version!	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMe251173	XM_021231159.1 Latest version!	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMe130621	XM_005077017.2	Mesocricetus auratus sperm associated antigen 5 (Spag5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag5 ( XM_005077017.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005077074.1 Mesocricetus auratus sperm associated antigen 5 (Spag5), mRNA.

Price & Availability↑

CloneID	OMe130621
Clone ID Related Accession (Same CDS sequence)	XM_005077017.2 , XM_005077017.3
Accession Version	XM_005077017.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	sperm-associated antigen 5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005077017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGAGACC CCAGGCCTGG AGAAGAGCCC AGCAGAAATG 
AGCACTATGT TACTGGAGCT TCAGAACCTG TGTTCTCTGC TGCAGGAGTC TAAAGAAGAA 
GCCGTTGGGG CCCTGCAGAG AAAAATCTGT GAACTGCAGA CTAGACTACA GGACCAAGAA 
GAAGAGCATC AGGAAGCCCT GAAGGCAAAG GAAGCAGACA TAGAAAAGCT GAACCAGGCC 
TTGTGCTTAT GCTACAAGAA TGAAAAGGAG CTCCAGGAAG TGATACAGAA GCAGAACGAG 
AAGATCTTAG GGCAGATAGA CAAGAGTGGC GAGCTCATAA GTCTCAAGGA GGAGGTGACC 
CAGCTCACAC GTTTACTGCG ACATGCAGAG ACAGAGACGA AAGTGCTCCA GGAAGCTCTG 
GAGGGCCAGC TAGACCCCAG CTGTCAGCTG ATGGCTACTA ACTGGATCCA GGAAAAAGTG 
TCTCTGTCAC AGGAGGTGGA AAAACTGAGG GCCATGTTCC TGGAGATGAA GAGTGAGAAA 
GCAAAGCTGG TGATGAAGTA CCAGAGCCAT AGGAACATTC TTGAAGAGAA CCTTCGGCGC 
TCTGACACTG AGTTAAAGAA ACTCGATGAC ACTATCCAGC ATATCTATGA GACTTTGCTG 
TCTGTCCCAG AGGTGGTGAA GAGTTGCAAG GAGCTACAGA GATTGCTGGA GTTTCTGAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005077074.1
CDS	46..3588
Translation	MWRLKTLNLGLSPSPQPGKPAMRTPLRELKLQPGALTDSGKGPPMFSSLTPYLRKLELKERSNNSSPVDFISTENSFLSEQFSPSTKYIEACQHESEPTPESSSPLSTAEKAVETMDDFVSDQVDESLVNSMVLLPFPLGQQQDLTLEAHLDAMAEINSSSLNESLRLEDLVEKEVAPCVEDSFTEVVAVRPEQPAFQDPPSHLLDCLPKPCSEQFHCSKENLRGINTEAASENLVPSENVLNFSVACLSPSTVDHVDPGKETVEYRVTQGSEKSFPTCPEEVELGDQALAAKSEDAASMYLTPSLVEMGPLEALGPTTEDASRIPGLDAETWMSPLAWLEKSVNTSVMLQNLRQRLSFPSVLQDAAAGNTPLSTCSVGTSFTPPVPPEVGTKHSTSETERHLLGCGPLDLTALSRHDLEENLLNSLVVLEVLSHQLQAWKKQLIVPSRESQDSSTQTDCSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWMLVSRELISLLHLSLTHMNEDTMTVSQESRRAETLVSSCSHVLKKLRAKLQSLRTEREEARHREEMALHDKDAAEAVLEAFRAHASQRISQLEQDLTSMREFRGLLQEAQTQLIGLHTEQEDLAQQTVSLASALQQDWTSMQQDYETWAALLSRSGELTEKLTTRSRQALQERDAAVEEKKQVLKELEQVSAHLEDCKGQTEQLKSENSRLTTDLWAQLQNLTSTDNQLKELQSQHAHCAQDLAMKDEVLCQLNQSIKEQAAQWQQEEMELKRTQAELQHQQAVLAKEVQDLKETLEFADQEGQVAHLELGQIECQLKATLEVLRERSLQCETLKDTVENLKAELASTVAKHEKQALKKTYQHSQELRLLTEQLQSLTLFLQAKLKENKAESEIILPSTGCAPAQEHPLPNDSTASENTLTAVADEEPEPVPIMPLLGSDKSAFTRVASTISLQPAETPGLEKSPAEMSTMLLELQNLCSLLQESKEEAVGALQRKICELQTRLQDQEEEHQEALKAKEADIEKLNQALCLCYKNEKELQEVIQKQNEKILGQIDKSGELISLKEEVTQLTRLLRHAETETKVLQEALEGQLDPSCQLMATNWIQEKVSLSQEVEKLRAMFLEMKSEKAKLVMKYQSHRNILEENLRRSDTELKKLDDTIQHIYETLLSVPEVVKSCKELQRLLEFLS

Target ORF information:

RefSeq Version	XM_005077017.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus sperm associated antigen 5 (Spag5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005077017.2

ORF Insert Sequence:

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGAGACC CCAGGCCTGG AGAAGAGCCC AGCAGAAATG 
AGCACTATGT TACTGGAGCT TCAGAACCTG TGTTCTCTGC TGCAGGAGTC TAAAGAAGAA 
GCCGTTGGGG CCCTGCAGAG AAAAATCTGT GAACTGCAGA CTAGACTACA GGACCAAGAA 
GAAGAGCATC AGGAAGCCCT GAAGGCAAAG GAAGCAGACA TAGAAAAGCT GAACCAGGCC 
TTGTGCTTAT GCTACAAGAA TGAAAAGGAG CTCCAGGAAG TGATACAGAA GCAGAACGAG 
AAGATCTTAG GGCAGATAGA CAAGAGTGGC GAGCTCATAA GTCTCAAGGA GGAGGTGACC 
CAGCTCACAC GTTTACTGCG ACATGCAGAG ACAGAGACGA AAGTGCTCCA GGAAGCTCTG 
GAGGGCCAGC TAGACCCCAG CTGTCAGCTG ATGGCTACTA ACTGGATCCA GGAAAAAGTG 
TCTCTGTCAC AGGAGGTGGA AAAACTGAGG GCCATGTTCC TGGAGATGAA GAGTGAGAAA 
GCAAAGCTGG TGATGAAGTA CCAGAGCCAT AGGAACATTC TTGAAGAGAA CCTTCGGCGC 
TCTGACACTG AGTTAAAGAA ACTCGATGAC ACTATCCAGC ATATCTATGA GACTTTGCTG 
TCTGTCCCAG AGGTGGTGAA GAGTTGCAAG GAGCTACAGA GATTGCTGGA GTTTCTGAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_005077017.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005077074.1 Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe130621
Clone ID Related Accession (Same CDS sequence)	XM_005077017.2 , XM_005077017.3
Accession Version	XM_005077017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	sperm-associated antigen 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005077017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGAGACC CCAGGCCTGG AGAAGAGCCC AGCAGAAATG 
AGCACTATGT TACTGGAGCT TCAGAACCTG TGTTCTCTGC TGCAGGAGTC TAAAGAAGAA 
GCCGTTGGGG CCCTGCAGAG AAAAATCTGT GAACTGCAGA CTAGACTACA GGACCAAGAA 
GAAGAGCATC AGGAAGCCCT GAAGGCAAAG GAAGCAGACA TAGAAAAGCT GAACCAGGCC 
TTGTGCTTAT GCTACAAGAA TGAAAAGGAG CTCCAGGAAG TGATACAGAA GCAGAACGAG 
AAGATCTTAG GGCAGATAGA CAAGAGTGGC GAGCTCATAA GTCTCAAGGA GGAGGTGACC 
CAGCTCACAC GTTTACTGCG ACATGCAGAG ACAGAGACGA AAGTGCTCCA GGAAGCTCTG 
GAGGGCCAGC TAGACCCCAG CTGTCAGCTG ATGGCTACTA ACTGGATCCA GGAAAAAGTG 
TCTCTGTCAC AGGAGGTGGA AAAACTGAGG GCCATGTTCC TGGAGATGAA GAGTGAGAAA 
GCAAAGCTGG TGATGAAGTA CCAGAGCCAT AGGAACATTC TTGAAGAGAA CCTTCGGCGC 
TCTGACACTG AGTTAAAGAA ACTCGATGAC ACTATCCAGC ATATCTATGA GACTTTGCTG 
TCTGTCCCAG AGGTGGTGAA GAGTTGCAAG GAGCTACAGA GATTGCTGGA GTTTCTGAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005077074.1
CDS	87..3629
Translation	MWRLKTLNLGLSPSPQPGKPAMRTPLRELKLQPGALTDSGKGPPMFSSLTPYLRKLELKERSNNSSPVDFISTENSFLSEQFSPSTKYIEACQHESEPTPESSSPLSTAEKAVETMDDFVSDQVDESLVNSMVLLPFPLGQQQDLTLEAHLDAMAEINSSSLNESLRLEDLVEKEVAPCVEDSFTEVVAVRPEQPAFQDPPSHLLDCLPKPCSEQFHCSKENLRGINTEAASENLVPSENVLNFSVACLSPSTVDHVDPGKETVEYRVTQGSEKSFPTCPEEVELGDQALAAKSEDAASMYLTPSLVEMGPLEALGPTTEDASRIPGLDAETWMSPLAWLEKSVNTSVMLQNLRQRLSFPSVLQDAAAGNTPLSTCSVGTSFTPPVPPEVGTKHSTSETERHLLGCGPLDLTALSRHDLEENLLNSLVVLEVLSHQLQAWKKQLIVPSRESQDSSTQTDCSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWMLVSRELISLLHLSLTHMNEDTMTVSQESRRAETLVSSCSHVLKKLRAKLQSLRTEREEARHREEMALHDKDAAEAVLEAFRAHASQRISQLEQDLTSMREFRGLLQEAQTQLIGLHTEQEDLAQQTVSLASALQQDWTSMQQDYETWAALLSRSGELTEKLTTRSRQALQERDAAVEEKKQVLKELEQVSAHLEDCKGQTEQLKSENSRLTTDLWAQLQNLTSTDNQLKELQSQHAHCAQDLAMKDEVLCQLNQSIKEQAAQWQQEEMELKRTQAELQHQQAVLAKEVQDLKETLEFADQEGQVAHLELGQIECQLKATLEVLRERSLQCETLKDTVENLKAELASTVAKHEKQALKKTYQHSQELRLLTEQLQSLTLFLQAKLKENKAESEIILPSTGCAPAQEHPLPNDSTASENTLTAVADEEPEPVPIMPLLGSDKSAFTRVASTISLQPAETPGLEKSPAEMSTMLLELQNLCSLLQESKEEAVGALQRKICELQTRLQDQEEEHQEALKAKEADIEKLNQALCLCYKNEKELQEVIQKQNEKILGQIDKSGELISLKEEVTQLTRLLRHAETETKVLQEALEGQLDPSCQLMATNWIQEKVSLSQEVEKLRAMFLEMKSEKAKLVMKYQSHRNILEENLRRSDTELKKLDDTIQHIYETLLSVPEVVKSCKELQRLLEFLS

Target ORF information:

RefSeq Version	XM_005077017.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005077017.3

ORF Insert Sequence:

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGAGACC CCAGGCCTGG AGAAGAGCCC AGCAGAAATG 
AGCACTATGT TACTGGAGCT TCAGAACCTG TGTTCTCTGC TGCAGGAGTC TAAAGAAGAA 
GCCGTTGGGG CCCTGCAGAG AAAAATCTGT GAACTGCAGA CTAGACTACA GGACCAAGAA 
GAAGAGCATC AGGAAGCCCT GAAGGCAAAG GAAGCAGACA TAGAAAAGCT GAACCAGGCC 
TTGTGCTTAT GCTACAAGAA TGAAAAGGAG CTCCAGGAAG TGATACAGAA GCAGAACGAG 
AAGATCTTAG GGCAGATAGA CAAGAGTGGC GAGCTCATAA GTCTCAAGGA GGAGGTGACC 
CAGCTCACAC GTTTACTGCG ACATGCAGAG ACAGAGACGA AAGTGCTCCA GGAAGCTCTG 
GAGGGCCAGC TAGACCCCAG CTGTCAGCTG ATGGCTACTA ACTGGATCCA GGAAAAAGTG 
TCTCTGTCAC AGGAGGTGGA AAAACTGAGG GCCATGTTCC TGGAGATGAA GAGTGAGAAA 
GCAAAGCTGG TGATGAAGTA CCAGAGCCAT AGGAACATTC TTGAAGAGAA CCTTCGGCGC 
TCTGACACTG AGTTAAAGAA ACTCGATGAC ACTATCCAGC ATATCTATGA GACTTTGCTG 
TCTGTCCCAG AGGTGGTGAA GAGTTGCAAG GAGCTACAGA GATTGCTGGA GTTTCTGAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_021231157.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021086816.1 Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe251171
Clone ID Related Accession (Same CDS sequence)	XM_021231157.1
Accession Version	XM_021231157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGGTGC CTGTGATACT TTTGATCTAG ATCTCTGGGC TCAGCTGCAG 
AATCTAACTA GCACAGACAA CCAACTAAAG GAACTGCAGA GTCAGCATGC CCACTGTGCC 
CAGGACCTGG CCATGAAGGA TGAGGTGTTG TGTCAACTTA ACCAGAGCAT CAAGGAGCAG 
GCTGCACAAT GGCAACAGGA AGAAATGGAA CTGAAGCGCA CACAAGCAGA GCTACAGCAT 
CAGCAGGCTG TGCTGGCTAA GGAGGTACAG GATCTGAAGG AGACCTTGGA GTTTGCAGAC 
CAAGAGGGTC AAGTCGCTCA CCTGGAGCTG GGCCAGATTG AGTGTCAGCT GAAAGCCACA 
CTAGAAGTGC TGCGGGAGCG CAGCCTGCAA TGCGAGACCC TCAAGGACAC TGTGGAGAAC 
CTGAAGGCTG AGCTGGCCAG CACTGTAGCG AAGCATGAGA AACAGGCTCT GAAGAAGACA 
TACCAGCATT CTCAAGAGCT GAGACTGCTG ACCGAGCAGC TACAGAGTCT CACCCTCTTC 
TTACAGGCAA AACTAAAGGA GAACAAGGCT GAATCAGAGA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCTGCCC AATGATAGCA CTGCTTCAGA AAACACCCTG 
ACGGCAGTGG CAGATGAAGA GCCAGAACCA GTTCCCATCA TGCCATTGCT TGGAAGTGAC 
AAGAGTGCTT TCACCCGAGT AGCATCAACA ATTTCCCTTC AGCCTGCAGG ACTTCCTCTT 
TCAGAGACCC CAGGCCTGGA GAAGAGCCCA GCAGAAATGA GCACTATGTT ACTGGAGCTT 
CAGAACCTGT GTTCTCTGCT GCAGGAGTCT AAAGAAGAAG CCGTTGGGGC CCTGCAGAGA 
AAAATCTGTG AACTGCAGAC TAGACTACAG GACCAAGAAG AAGAGCATCA GGAAGCCCTG 
AAGGCAAAGG AAGCAGACAT AGAAAAGCTG AACCAGGCCT TGTGCTTATG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGAAG CAGAACGAGA AGATCTTAGG GCAGATAGAC 
AAGAGTGGCG AGCTCATAAG TCTCAAGGAG GAGGTGACCC AGCTCACACG TTTACTGCGA 
CATGCAGAGA CAGAGACGAA AGTGCTCCAG GAAGCTCTGG AGGGCCAGCT AGACCCCAGC 
TGTCAGCTGA TGGCTACTAA CTGGATCCAG GAAAAAGTGT CTCTGTCACA GGAGGTGGAA 
AAACTGAGGG CCATGTTCCT GGAGATGAAG AGTGAGAAAG CAAAGCTGGT GATGAAGTAC 
CAGAGCCATA GGAACATTCT TGAAGAGAAC CTTCGGCGCT CTGACACTGA GTTAAAGAAA 
CTCGATGACA CTATCCAGCA TATCTATGAG ACTTTGCTGT CTGTCCCAGA GGTGGTGAAG 
AGTTGCAAGG AGCTACAGAG ATTGCTGGAG TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021086816.1
CDS	87..3668
Translation	MWRLKTLNLGLSPSPQPGKPAMRTPLRELKLQPGALTDSGKGPPMFSSLTPYLRKLELKERSNNSSPVDFISTENSFLSEQFSPSTKYIEACQHESEPTPESSSPLSTAEKAVETMDDFVSDQVDESLVNSMVLLPFPLGQQQDLTLEAHLDAMAEINSSSLNESLRLEDLVEKEVAPCVEDSFTEVVAVRPEQPAFQDPPSHLLDCLPKPCSEQFHCSKENLRGINTEAASENLVPSENVLNFSVACLSPSTVDHVDPGKETVEYRVTQGSEKSFPTCPEEVELGDQALAAKSEDAASMYLTPSLVEMGPLEALGPTTEDASRIPGLDAETWMSPLAWLEKSVNTSVMLQNLRQRLSFPSVLQDAAAGNTPLSTCSVGTSFTPPVPPEVGTKHSTSETERHLLGCGPLDLTALSRHDLEENLLNSLVVLEVLSHQLQAWKKQLIVPSRESQDSSTQTDCSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWMLVSRELISLLHLSLTHMNEDTMTVSQESRRAETLVSSCSHVLKKLRAKLQSLRTEREEARHREEMALHDKDAAEAVLEAFRAHASQRISQLEQDLTSMREFRGLLQEAQTQLIGLHTEQEDLAQQTVSLASALQQDWTSMQQDYETWAALLSRSGELTEKLTTRSRQALQERDAAVEEKKQVLKELEQVSAHLEDCKGQTEQLKSENSRLTTGACDTFDLDLWAQLQNLTSTDNQLKELQSQHAHCAQDLAMKDEVLCQLNQSIKEQAAQWQQEEMELKRTQAELQHQQAVLAKEVQDLKETLEFADQEGQVAHLELGQIECQLKATLEVLRERSLQCETLKDTVENLKAELASTVAKHEKQALKKTYQHSQELRLLTEQLQSLTLFLQAKLKENKAESEIILPSTGCAPAQEHPLPNDSTASENTLTAVADEEPEPVPIMPLLGSDKSAFTRVASTISLQPAGLPLSETPGLEKSPAEMSTMLLELQNLCSLLQESKEEAVGALQRKICELQTRLQDQEEEHQEALKAKEADIEKLNQALCLCYKNEKELQEVIQKQNEKILGQIDKSGELISLKEEVTQLTRLLRHAETETKVLQEALEGQLDPSCQLMATNWIQEKVSLSQEVEKLRAMFLEMKSEKAKLVMKYQSHRNILEENLRRSDTELKKLDDTIQHIYETLLSVPEVVKSCKELQRLLEFLS

Target ORF information:

RefSeq Version	XM_021231157.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021231157.1

ORF Insert Sequence:

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGGTGC CTGTGATACT TTTGATCTAG ATCTCTGGGC TCAGCTGCAG 
AATCTAACTA GCACAGACAA CCAACTAAAG GAACTGCAGA GTCAGCATGC CCACTGTGCC 
CAGGACCTGG CCATGAAGGA TGAGGTGTTG TGTCAACTTA ACCAGAGCAT CAAGGAGCAG 
GCTGCACAAT GGCAACAGGA AGAAATGGAA CTGAAGCGCA CACAAGCAGA GCTACAGCAT 
CAGCAGGCTG TGCTGGCTAA GGAGGTACAG GATCTGAAGG AGACCTTGGA GTTTGCAGAC 
CAAGAGGGTC AAGTCGCTCA CCTGGAGCTG GGCCAGATTG AGTGTCAGCT GAAAGCCACA 
CTAGAAGTGC TGCGGGAGCG CAGCCTGCAA TGCGAGACCC TCAAGGACAC TGTGGAGAAC 
CTGAAGGCTG AGCTGGCCAG CACTGTAGCG AAGCATGAGA AACAGGCTCT GAAGAAGACA 
TACCAGCATT CTCAAGAGCT GAGACTGCTG ACCGAGCAGC TACAGAGTCT CACCCTCTTC 
TTACAGGCAA AACTAAAGGA GAACAAGGCT GAATCAGAGA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCTGCCC AATGATAGCA CTGCTTCAGA AAACACCCTG 
ACGGCAGTGG CAGATGAAGA GCCAGAACCA GTTCCCATCA TGCCATTGCT TGGAAGTGAC 
AAGAGTGCTT TCACCCGAGT AGCATCAACA ATTTCCCTTC AGCCTGCAGG ACTTCCTCTT 
TCAGAGACCC CAGGCCTGGA GAAGAGCCCA GCAGAAATGA GCACTATGTT ACTGGAGCTT 
CAGAACCTGT GTTCTCTGCT GCAGGAGTCT AAAGAAGAAG CCGTTGGGGC CCTGCAGAGA 
AAAATCTGTG AACTGCAGAC TAGACTACAG GACCAAGAAG AAGAGCATCA GGAAGCCCTG 
AAGGCAAAGG AAGCAGACAT AGAAAAGCTG AACCAGGCCT TGTGCTTATG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGAAG CAGAACGAGA AGATCTTAGG GCAGATAGAC 
AAGAGTGGCG AGCTCATAAG TCTCAAGGAG GAGGTGACCC AGCTCACACG TTTACTGCGA 
CATGCAGAGA CAGAGACGAA AGTGCTCCAG GAAGCTCTGG AGGGCCAGCT AGACCCCAGC 
TGTCAGCTGA TGGCTACTAA CTGGATCCAG GAAAAAGTGT CTCTGTCACA GGAGGTGGAA 
AAACTGAGGG CCATGTTCCT GGAGATGAAG AGTGAGAAAG CAAAGCTGGT GATGAAGTAC 
CAGAGCCATA GGAACATTCT TGAAGAGAAC CTTCGGCGCT CTGACACTGA GTTAAAGAAA 
CTCGATGACA CTATCCAGCA TATCTATGAG ACTTTGCTGT CTGTCCCAGA GGTGGTGAAG 
AGTTGCAAGG AGCTACAGAG ATTGCTGGAG TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_021231158.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021086817.1 Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe251172
Clone ID Related Accession (Same CDS sequence)	XM_021231158.1
Accession Version	XM_021231158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGGTGC CTGTGATACT TTTGATCTAG ATCTCTGGGC TCAGCTGCAG 
AATCTAACTA GCACAGACAA CCAACTAAAG GAACTGCAGA GTCAGCATGC CCACTGTGCC 
CAGGACCTGG CCATGAAGGA TGAGGTGTTG TGTCAACTTA ACCAGAGCAT CAAGGAGCAG 
GCTGCACAAT GGCAACAGGA AGAAATGGAA CTGAAGCGCA CACAAGCAGA GCTACAGCAT 
CAGCAGGCTG TGCTGGCTAA GGAGGTACAG GATCTGAAGG AGACCTTGGA GTTTGCAGAC 
CAAGAGGGTC AAGTCGCTCA CCTGGAGCTG GGCCAGATTG AGTGTCAGCT GAAAGCCACA 
CTAGAAGTGC TGCGGGAGCG CAGCCTGCAA TGCGAGACCC TCAAGGACAC TGTGGAGAAC 
CTGAAGGCTG AGCTGGCCAG CACTGTAGCG AAGCATGAGA AACAGGCTCT GAAGAAGACA 
TACCAGCATT CTCAAGAGCT GAGACTGCTG ACCGAGCAGC TACAGAGTCT CACCCTCTTC 
TTACAGGCAA AACTAAAGGA GAACAAGGCT GAATCAGAGA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCTGCCC AATGATAGCA CTGCTTCAGA AAACACCCTG 
ACGGCAGTGG CAGATGAAGA GCCAGAACCA GTTCCCATCA TGCCATTGCT TGGAAGTGAC 
AAGAGTGCTT TCACCCGAGT AGCATCAACA ATTTCCCTTC AGCCTGCAGA GACCCCAGGC 
CTGGAGAAGA GCCCAGCAGA AATGAGCACT ATGTTACTGG AGCTTCAGAA CCTGTGTTCT 
CTGCTGCAGG AGTCTAAAGA AGAAGCCGTT GGGGCCCTGC AGAGAAAAAT CTGTGAACTG 
CAGACTAGAC TACAGGACCA AGAAGAAGAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAAA AGCTGAACCA GGCCTTGTGC TTATGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGAAGCAGAA CGAGAAGATC TTAGGGCAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTCA AGGAGGAGGT GACCCAGCTC ACACGTTTAC TGCGACATGC AGAGACAGAG 
ACGAAAGTGC TCCAGGAAGC TCTGGAGGGC CAGCTAGACC CCAGCTGTCA GCTGATGGCT 
ACTAACTGGA TCCAGGAAAA AGTGTCTCTG TCACAGGAGG TGGAAAAACT GAGGGCCATG 
TTCCTGGAGA TGAAGAGTGA GAAAGCAAAG CTGGTGATGA AGTACCAGAG CCATAGGAAC 
ATTCTTGAAG AGAACCTTCG GCGCTCTGAC ACTGAGTTAA AGAAACTCGA TGACACTATC 
CAGCATATCT ATGAGACTTT GCTGTCTGTC CCAGAGGTGG TGAAGAGTTG CAAGGAGCTA 
CAGAGATTGC TGGAGTTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021086817.1
CDS	87..3653
Translation	MWRLKTLNLGLSPSPQPGKPAMRTPLRELKLQPGALTDSGKGPPMFSSLTPYLRKLELKERSNNSSPVDFISTENSFLSEQFSPSTKYIEACQHESEPTPESSSPLSTAEKAVETMDDFVSDQVDESLVNSMVLLPFPLGQQQDLTLEAHLDAMAEINSSSLNESLRLEDLVEKEVAPCVEDSFTEVVAVRPEQPAFQDPPSHLLDCLPKPCSEQFHCSKENLRGINTEAASENLVPSENVLNFSVACLSPSTVDHVDPGKETVEYRVTQGSEKSFPTCPEEVELGDQALAAKSEDAASMYLTPSLVEMGPLEALGPTTEDASRIPGLDAETWMSPLAWLEKSVNTSVMLQNLRQRLSFPSVLQDAAAGNTPLSTCSVGTSFTPPVPPEVGTKHSTSETERHLLGCGPLDLTALSRHDLEENLLNSLVVLEVLSHQLQAWKKQLIVPSRESQDSSTQTDCSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWMLVSRELISLLHLSLTHMNEDTMTVSQESRRAETLVSSCSHVLKKLRAKLQSLRTEREEARHREEMALHDKDAAEAVLEAFRAHASQRISQLEQDLTSMREFRGLLQEAQTQLIGLHTEQEDLAQQTVSLASALQQDWTSMQQDYETWAALLSRSGELTEKLTTRSRQALQERDAAVEEKKQVLKELEQVSAHLEDCKGQTEQLKSENSRLTTGACDTFDLDLWAQLQNLTSTDNQLKELQSQHAHCAQDLAMKDEVLCQLNQSIKEQAAQWQQEEMELKRTQAELQHQQAVLAKEVQDLKETLEFADQEGQVAHLELGQIECQLKATLEVLRERSLQCETLKDTVENLKAELASTVAKHEKQALKKTYQHSQELRLLTEQLQSLTLFLQAKLKENKAESEIILPSTGCAPAQEHPLPNDSTASENTLTAVADEEPEPVPIMPLLGSDKSAFTRVASTISLQPAETPGLEKSPAEMSTMLLELQNLCSLLQESKEEAVGALQRKICELQTRLQDQEEEHQEALKAKEADIEKLNQALCLCYKNEKELQEVIQKQNEKILGQIDKSGELISLKEEVTQLTRLLRHAETETKVLQEALEGQLDPSCQLMATNWIQEKVSLSQEVEKLRAMFLEMKSEKAKLVMKYQSHRNILEENLRRSDTELKKLDDTIQHIYETLLSVPEVVKSCKELQRLLEFLS

Target ORF information:

RefSeq Version	XM_021231158.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021231158.1

ORF Insert Sequence:

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGGTGC CTGTGATACT TTTGATCTAG ATCTCTGGGC TCAGCTGCAG 
AATCTAACTA GCACAGACAA CCAACTAAAG GAACTGCAGA GTCAGCATGC CCACTGTGCC 
CAGGACCTGG CCATGAAGGA TGAGGTGTTG TGTCAACTTA ACCAGAGCAT CAAGGAGCAG 
GCTGCACAAT GGCAACAGGA AGAAATGGAA CTGAAGCGCA CACAAGCAGA GCTACAGCAT 
CAGCAGGCTG TGCTGGCTAA GGAGGTACAG GATCTGAAGG AGACCTTGGA GTTTGCAGAC 
CAAGAGGGTC AAGTCGCTCA CCTGGAGCTG GGCCAGATTG AGTGTCAGCT GAAAGCCACA 
CTAGAAGTGC TGCGGGAGCG CAGCCTGCAA TGCGAGACCC TCAAGGACAC TGTGGAGAAC 
CTGAAGGCTG AGCTGGCCAG CACTGTAGCG AAGCATGAGA AACAGGCTCT GAAGAAGACA 
TACCAGCATT CTCAAGAGCT GAGACTGCTG ACCGAGCAGC TACAGAGTCT CACCCTCTTC 
TTACAGGCAA AACTAAAGGA GAACAAGGCT GAATCAGAGA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCTGCCC AATGATAGCA CTGCTTCAGA AAACACCCTG 
ACGGCAGTGG CAGATGAAGA GCCAGAACCA GTTCCCATCA TGCCATTGCT TGGAAGTGAC 
AAGAGTGCTT TCACCCGAGT AGCATCAACA ATTTCCCTTC AGCCTGCAGA GACCCCAGGC 
CTGGAGAAGA GCCCAGCAGA AATGAGCACT ATGTTACTGG AGCTTCAGAA CCTGTGTTCT 
CTGCTGCAGG AGTCTAAAGA AGAAGCCGTT GGGGCCCTGC AGAGAAAAAT CTGTGAACTG 
CAGACTAGAC TACAGGACCA AGAAGAAGAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAAA AGCTGAACCA GGCCTTGTGC TTATGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGAAGCAGAA CGAGAAGATC TTAGGGCAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTCA AGGAGGAGGT GACCCAGCTC ACACGTTTAC TGCGACATGC AGAGACAGAG 
ACGAAAGTGC TCCAGGAAGC TCTGGAGGGC CAGCTAGACC CCAGCTGTCA GCTGATGGCT 
ACTAACTGGA TCCAGGAAAA AGTGTCTCTG TCACAGGAGG TGGAAAAACT GAGGGCCATG 
TTCCTGGAGA TGAAGAGTGA GAAAGCAAAG CTGGTGATGA AGTACCAGAG CCATAGGAAC 
ATTCTTGAAG AGAACCTTCG GCGCTCTGAC ACTGAGTTAA AGAAACTCGA TGACACTATC 
CAGCATATCT ATGAGACTTT GCTGTCTGTC CCAGAGGTGG TGAAGAGTTG CAAGGAGCTA 
CAGAGATTGC TGGAGTTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_021231159.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021086818.1 Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

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CloneID	OMe251173
Clone ID Related Accession (Same CDS sequence)	XM_021231159.1
Accession Version	XM_021231159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	sperm-associated antigen 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGGACTT CCTCTTTCAG AGACCCCAGG CCTGGAGAAG 
AGCCCAGCAG AAATGAGCAC TATGTTACTG GAGCTTCAGA ACCTGTGTTC TCTGCTGCAG 
GAGTCTAAAG AAGAAGCCGT TGGGGCCCTG CAGAGAAAAA TCTGTGAACT GCAGACTAGA 
CTACAGGACC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATAGAA 
AAGCTGAACC AGGCCTTGTG CTTATGCTAC AAGAATGAAA AGGAGCTCCA GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CTTAGGGCAG ATAGACAAGA GTGGCGAGCT CATAAGTCTC 
AAGGAGGAGG TGACCCAGCT CACACGTTTA CTGCGACATG CAGAGACAGA GACGAAAGTG 
CTCCAGGAAG CTCTGGAGGG CCAGCTAGAC CCCAGCTGTC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTCTCT GTCACAGGAG GTGGAAAAAC TGAGGGCCAT GTTCCTGGAG 
ATGAAGAGTG AGAAAGCAAA GCTGGTGATG AAGTACCAGA GCCATAGGAA CATTCTTGAA 
GAGAACCTTC GGCGCTCTGA CACTGAGTTA AAGAAACTCG ATGACACTAT CCAGCATATC 
TATGAGACTT TGCTGTCTGT CCCAGAGGTG GTGAAGAGTT GCAAGGAGCT ACAGAGATTG 
CTGGAGTTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021086818.1
CDS	87..3644
Translation	MWRLKTLNLGLSPSPQPGKPAMRTPLRELKLQPGALTDSGKGPPMFSSLTPYLRKLELKERSNNSSPVDFISTENSFLSEQFSPSTKYIEACQHESEPTPESSSPLSTAEKAVETMDDFVSDQVDESLVNSMVLLPFPLGQQQDLTLEAHLDAMAEINSSSLNESLRLEDLVEKEVAPCVEDSFTEVVAVRPEQPAFQDPPSHLLDCLPKPCSEQFHCSKENLRGINTEAASENLVPSENVLNFSVACLSPSTVDHVDPGKETVEYRVTQGSEKSFPTCPEEVELGDQALAAKSEDAASMYLTPSLVEMGPLEALGPTTEDASRIPGLDAETWMSPLAWLEKSVNTSVMLQNLRQRLSFPSVLQDAAAGNTPLSTCSVGTSFTPPVPPEVGTKHSTSETERHLLGCGPLDLTALSRHDLEENLLNSLVVLEVLSHQLQAWKKQLIVPSRESQDSSTQTDCSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWMLVSRELISLLHLSLTHMNEDTMTVSQESRRAETLVSSCSHVLKKLRAKLQSLRTEREEARHREEMALHDKDAAEAVLEAFRAHASQRISQLEQDLTSMREFRGLLQEAQTQLIGLHTEQEDLAQQTVSLASALQQDWTSMQQDYETWAALLSRSGELTEKLTTRSRQALQERDAAVEEKKQVLKELEQVSAHLEDCKGQTEQLKSENSRLTTDLWAQLQNLTSTDNQLKELQSQHAHCAQDLAMKDEVLCQLNQSIKEQAAQWQQEEMELKRTQAELQHQQAVLAKEVQDLKETLEFADQEGQVAHLELGQIECQLKATLEVLRERSLQCETLKDTVENLKAELASTVAKHEKQALKKTYQHSQELRLLTEQLQSLTLFLQAKLKENKAESEIILPSTGCAPAQEHPLPNDSTASENTLTAVADEEPEPVPIMPLLGSDKSAFTRVASTISLQPAGLPLSETPGLEKSPAEMSTMLLELQNLCSLLQESKEEAVGALQRKICELQTRLQDQEEEHQEALKAKEADIEKLNQALCLCYKNEKELQEVIQKQNEKILGQIDKSGELISLKEEVTQLTRLLRHAETETKVLQEALEGQLDPSCQLMATNWIQEKVSLSQEVEKLRAMFLEMKSEKAKLVMKYQSHRNILEENLRRSDTELKKLDDTIQHIYETLLSVPEVVKSCKELQRLLEFLS

Target ORF information:

RefSeq Version	XM_021231159.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021231159.1

ORF Insert Sequence:

ATGTGGAGGC TTAAAACACT GAATCTCGGC TTGTCGCCTT CACCCCAACC GGGAAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTGCAGCCCG GAGCCCTCAC CGATTCAGGG 
AAAGGACCGC CGATGTTCTC CTCGCTGACC CCATACCTGC GCAAGCTGGA GCTGAAGGAA 
AGGAGCAACA ACTCATCTCC AGTGGATTTT ATCAGCACTG AGAACAGCTT CCTTTCAGAA 
CAGTTCAGCC CTTCAACAAA ATACATAGAA GCTTGTCAGC ATGAATCTGA ACCAACTCCT 
GAAAGCAGCT CTCCTCTCAG TACAGCAGAG AAAGCAGTAG AAACAATGGA TGATTTTGTA 
TCAGATCAGG TGGATGAAAG TCTCGTTAAC TCCATGGTTC TTTTACCCTT TCCACTAGGG 
CAGCAGCAAG ACCTGACGCT TGAGGCTCAC TTAGATGCCA TGGCAGAGAT AAACAGCAGC 
TCTCTAAATG AGTCCTTGAG GTTAGAAGAT CTGGTGGAAA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCT TTACAGAAGT TGTTGCTGTT AGGCCTGAGC AACCTGCATT TCAGGATCCT 
CCATCTCATC TCTTGGACTG TCTACCCAAA CCCTGTTCTG AACAATTTCA CTGCTCCAAA 
GAAAACCTGA GAGGCATTAA CACTGAAGCT GCATCTGAGA ACTTAGTACC TTCTGAAAAT 
GTCTTGAATT TCTCTGTGGC CTGCCTTTCT CCCTCAACTG TTGATCATGT AGATCCAGGG 
AAGGAAACTG TAGAATACAG AGTTACACAG GGAAGTGAAA AAAGCTTTCC CACATGCCCT 
GAGGAGGTTG AATTGGGAGA CCAAGCACTT GCTGCAAAGT CAGAAGATGC TGCATCGATG 
TATCTGACGC CAAGTCTAGT AGAAATGGGA CCCCTGGAAG CTCTAGGCCC AACTACAGAA 
GATGCTAGTA GGATTCCTGG CTTAGATGCA GAGACTTGGA TGTCCCCATT GGCCTGGCTG 
GAAAAAAGTG TGAACACGTC AGTCATGCTA CAAAATCTCC GCCAGAGGTT ATCCTTTCCC 
TCTGTGCTTC AGGATGCTGC CGCTGGCAAC ACACCCCTCT CCACTTGTTC TGTGGGAACT 
TCTTTTACTC CTCCAGTGCC ACCAGAAGTC GGCACCAAGC ACAGTACCTC AGAGACGGAG 
CGCCACCTCT TAGGCTGCGG TCCTCTGGAT CTGACTGCTT TGTCCCGACA CGACTTGGAA 
GAGAACCTGT TGAACTCCCT TGTTGTGCTG GAAGTTCTTT CCCACCAGCT ACAAGCCTGG 
AAGAAGCAGC TCATCGTCCC TTCCCGGGAA TCTCAAGACA GTAGCACACA GACTGACTGC 
TCTCCTTGTG GGGTCACTAA GACACCTAAA CATCTTCAGG ACAGCAAAGA GATTAGACAG 
GCTTTGCTGC AAGCCAGGAA TGTCATGCAA TCATGGATGC TAGTCTCTAG AGAACTGATA 
TCCTTGCTTC ACCTGTCCCT GACACACATG AATGAAGACA CAATGACAGT GAGTCAGGAG 
TCTCGGCGTG CAGAAACCTT GGTTTCCTCC TGTTCTCATG TACTGAAGAA ACTAAGGGCT 
AAGCTCCAGA GCCTCAGAAC AGAAAGGGAG GAGGCAAGGC ACAGAGAGGA GATGGCCCTT 
CATGACAAAG ATGCGGCAGA GGCAGTACTG GAGGCTTTCC GTGCACATGC TAGCCAGCGC 
ATCAGCCAAC TGGAACAGGA TTTGACATCT ATGCGGGAGT TCAGAGGCCT TTTACAGGAG 
GCCCAGACCC AACTGATAGG GCTTCACACA GAACAAGAGG ATCTGGCTCA GCAGACAGTG 
AGTCTGGCTT CTGCTTTGCA GCAGGACTGG ACATCAATGC AACAGGATTA TGAAACATGG 
GCAGCTTTGC TGAGTCGATC TGGAGAACTC ACAGAGAAAC TCACAACCAG GAGCCGGCAG 
GCCCTTCAGG AACGTGATGC TGCAGTCGAG GAAAAGAAGC AGGTTTTGAA GGAACTTGAA 
CAAGTCTCTG CCCATTTAGA AGACTGTAAA GGCCAAACAG AACAATTGAA ATCGGAAAAT 
AGTCGTCTCA CCACAGATCT CTGGGCTCAG CTGCAGAATC TAACTAGCAC AGACAACCAA 
CTAAAGGAAC TGCAGAGTCA GCATGCCCAC TGTGCCCAGG ACCTGGCCAT GAAGGATGAG 
GTGTTGTGTC AACTTAACCA GAGCATCAAG GAGCAGGCTG CACAATGGCA ACAGGAAGAA 
ATGGAACTGA AGCGCACACA AGCAGAGCTA CAGCATCAGC AGGCTGTGCT GGCTAAGGAG 
GTACAGGATC TGAAGGAGAC CTTGGAGTTT GCAGACCAAG AGGGTCAAGT CGCTCACCTG 
GAGCTGGGCC AGATTGAGTG TCAGCTGAAA GCCACACTAG AAGTGCTGCG GGAGCGCAGC 
CTGCAATGCG AGACCCTCAA GGACACTGTG GAGAACCTGA AGGCTGAGCT GGCCAGCACT 
GTAGCGAAGC ATGAGAAACA GGCTCTGAAG AAGACATACC AGCATTCTCA AGAGCTGAGA 
CTGCTGACCG AGCAGCTACA GAGTCTCACC CTCTTCTTAC AGGCAAAACT AAAGGAGAAC 
AAGGCTGAAT CAGAGATCAT TCTGCCCAGC ACAGGCTGTG CTCCAGCCCA GGAACACCCT 
CTGCCCAATG ATAGCACTGC TTCAGAAAAC ACCCTGACGG CAGTGGCAGA TGAAGAGCCA 
GAACCAGTTC CCATCATGCC ATTGCTTGGA AGTGACAAGA GTGCTTTCAC CCGAGTAGCA 
TCAACAATTT CCCTTCAGCC TGCAGGACTT CCTCTTTCAG AGACCCCAGG CCTGGAGAAG 
AGCCCAGCAG AAATGAGCAC TATGTTACTG GAGCTTCAGA ACCTGTGTTC TCTGCTGCAG 
GAGTCTAAAG AAGAAGCCGT TGGGGCCCTG CAGAGAAAAA TCTGTGAACT GCAGACTAGA 
CTACAGGACC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATAGAA 
AAGCTGAACC AGGCCTTGTG CTTATGCTAC AAGAATGAAA AGGAGCTCCA GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CTTAGGGCAG ATAGACAAGA GTGGCGAGCT CATAAGTCTC 
AAGGAGGAGG TGACCCAGCT CACACGTTTA CTGCGACATG CAGAGACAGA GACGAAAGTG 
CTCCAGGAAG CTCTGGAGGG CCAGCTAGAC CCCAGCTGTC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTCTCT GTCACAGGAG GTGGAAAAAC TGAGGGCCAT GTTCCTGGAG 
ATGAAGAGTG AGAAAGCAAA GCTGGTGATG AAGTACCAGA GCCATAGGAA CATTCTTGAA 
GAGAACCTTC GGCGCTCTGA CACTGAGTTA AAGAAACTCG ATGACACTAT CCAGCATATC 
TATGAGACTT TGCTGTCTGT CCCAGAGGTG GTGAAGAGTT GCAAGGAGCT ACAGAGATTG 
CTGGAGTTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Spag5 cDNA ORF clone, Mesocricetus auratus(golden hamster)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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