Mylk cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk
Entrez Gene ID	101827753
Full Name	myosin light chain kinase
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021228014.1	XP_021083673.1	myosin light chain kinase, smooth muscle isoform X1
XM_005071528.2	XP_005071585.1	myosin light chain kinase, smooth muscle isoform X1
XM_005071528.3	XP_005071585.1	myosin light chain kinase, smooth muscle isoform X1
XM_013114546.1	XP_012970000.1	myosin light chain kinase, smooth muscle isoform X1
XM_013114547.1	XP_012970001.1	myosin light chain kinase, smooth muscle isoform X2
XM_013114547.2	XP_012970001.1	myosin light chain kinase, smooth muscle isoform X2
XM_021228015.1	XP_021083674.1	myosin light chain kinase, smooth muscle isoform X3

Pathways from BioSystems
Gene Ontology

The following Mylk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe248849	XM_021228015.1 Latest version!	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMe125131	XM_013114546.1 Latest version!	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe125131	XM_021228014.1 Latest version!	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe125131	XM_005071528.3 Latest version!	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe125132	XM_013114547.2 Latest version!	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMe125131	XM_005071528.2	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe125132	XM_013114547.1	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk ( XM_021228015.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021083674.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe248849
Clone ID Related Accession (Same CDS sequence)	XM_021228015.1
Accession Version	XM_021228015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTCTGC TAGGAACAGA GAGTGATGCA 
ACTGTAAAAA AGAAACCTGC ACCCAAGACA CCTACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGCGAGC CTGTGGAGCT GTTTGGAAAA 
GTGACTGGTA CCCAGCCCAT CACCTGCACA TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCACA TCAAGGTGGA GAGCAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCAGCA 
CGCCAGGAGC ACTGTGGCTG CTACACATTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACCGT CGTGGATAAG CCCGACCCCC CAGCTGGCAC ACCTTGTGCA 
TCTGACATAC GGAGTTCCTC GCTGACCCTG TCCTGGTACG GCTCTTCCTA TGATGGAGGC 
AGTGCTGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGAT GTGGAAAGAA 
CTAACCACAT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT 
AAATTCCGAG TACGTGCGGT CAACGTCTAT GGAACCAGCG AGCCGAGCCA GGAGTCTGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAGGAG CCAAAGGATG AAGTGGAAGT GTCAGATGAT 
GACGAAAAGG AGCCTGAAGT CGATTATCGG ACAGTGACAG TTAACACTGA ACAGAAAGTA 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAACCCTGG GCAGGGAAGT TTTTCAAGGC CTATTCTGCC 
AAGGAAAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATC 
GTGTCAGGGG GCGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTCGAGCT GACGGAGCGA 
GAGTGCATCA AGTACATGAG GCAGATTTCA GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG TCTGGCACGA AGACTCGAGA ATGCTGGGTC TCTGAAGGTC 
CTCTTCGGAA CCCCGGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAACC CATCGGCTAC 
GCTACAGACA TGTGGAGCAT TGGGGTCATC TGCTATATTC TGGTCAGCGG ACTGTCTCCC 
TTCATGGGTG ACAATGATAA TGAGACCTTA GCCAATGTCA CTTCTGCCAC CTGGGACTTT 
GATGATGAGG CGTTCGATGA AATCTCCGAC GATGCCAAGG ACTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACGCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGCATGAA GAAGTACATG 
GCAAGGAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CGGGGTCACC AACCAGCCCA 
ATCAATGCAG ACAAACTAGA GTCCGAAGAA GATGTCTCCC AGGCTTTCCT TGAGGCTGTT 
GCGGAAGAAA AGCCCCATGC GAAGCCCTTT TTCTCTAAGA CCATTCGTGA TCTGGAAGTT 
GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT 
GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGTC ACTTCCAGAT AGACTACGAT 
GAAGATGGGA ACTGCTCTCT CATTATTAGC GATGTCTGCG GGGATGATGA TGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATTGTGGAA 
ACCATGGAGG AAGGGGAAGG GGAAGGGGGA GAGGAAGAGG AGGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021083674.1
CDS	123..2273
Translation	MKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_021228015.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021228015.1

ORF Insert Sequence:

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTCTGC TAGGAACAGA GAGTGATGCA 
ACTGTAAAAA AGAAACCTGC ACCCAAGACA CCTACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGCGAGC CTGTGGAGCT GTTTGGAAAA 
GTGACTGGTA CCCAGCCCAT CACCTGCACA TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCACA TCAAGGTGGA GAGCAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCAGCA 
CGCCAGGAGC ACTGTGGCTG CTACACATTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACCGT CGTGGATAAG CCCGACCCCC CAGCTGGCAC ACCTTGTGCA 
TCTGACATAC GGAGTTCCTC GCTGACCCTG TCCTGGTACG GCTCTTCCTA TGATGGAGGC 
AGTGCTGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGAT GTGGAAAGAA 
CTAACCACAT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT 
AAATTCCGAG TACGTGCGGT CAACGTCTAT GGAACCAGCG AGCCGAGCCA GGAGTCTGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAGGAG CCAAAGGATG AAGTGGAAGT GTCAGATGAT 
GACGAAAAGG AGCCTGAAGT CGATTATCGG ACAGTGACAG TTAACACTGA ACAGAAAGTA 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAACCCTGG GCAGGGAAGT TTTTCAAGGC CTATTCTGCC 
AAGGAAAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATC 
GTGTCAGGGG GCGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTCGAGCT GACGGAGCGA 
GAGTGCATCA AGTACATGAG GCAGATTTCA GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG TCTGGCACGA AGACTCGAGA ATGCTGGGTC TCTGAAGGTC 
CTCTTCGGAA CCCCGGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAACC CATCGGCTAC 
GCTACAGACA TGTGGAGCAT TGGGGTCATC TGCTATATTC TGGTCAGCGG ACTGTCTCCC 
TTCATGGGTG ACAATGATAA TGAGACCTTA GCCAATGTCA CTTCTGCCAC CTGGGACTTT 
GATGATGAGG CGTTCGATGA AATCTCCGAC GATGCCAAGG ACTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACGCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGCATGAA GAAGTACATG 
GCAAGGAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CGGGGTCACC AACCAGCCCA 
ATCAATGCAG ACAAACTAGA GTCCGAAGAA GATGTCTCCC AGGCTTTCCT TGAGGCTGTT 
GCGGAAGAAA AGCCCCATGC GAAGCCCTTT TTCTCTAAGA CCATTCGTGA TCTGGAAGTT 
GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT 
GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGTC ACTTCCAGAT AGACTACGAT 
GAAGATGGGA ACTGCTCTCT CATTATTAGC GATGTCTGCG GGGATGATGA TGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATTGTGGAA 
ACCATGGAGG AAGGGGAAGG GGAAGGGGGA GAGGAAGAGG AGGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_021228014.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021083673.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe125131
Clone ID Related Accession (Same CDS sequence)	XM_021228014.1 , XM_005071528.2 , XM_005071528.3 , XM_013114546.1
Accession Version	XM_021228014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021083673.1
CDS	230..6061
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_021228014.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021228014.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_005071528.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005071585.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

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CloneID	OMe125131
Clone ID Related Accession (Same CDS sequence)	XM_021228014.1 , XM_005071528.2 , XM_005071528.3 , XM_013114546.1
Accession Version	XM_005071528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005071528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005071585.1
CDS	178..6009
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_005071528.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005071528.2

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_005071528.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005071585.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

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CloneID	OMe125131
Clone ID Related Accession (Same CDS sequence)	XM_021228014.1 , XM_005071528.2 , XM_005071528.3 , XM_013114546.1
Accession Version	XM_005071528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005071528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005071585.1
CDS	153..5984
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_005071528.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005071528.3

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_013114546.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012970000.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

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CloneID	OMe125131
Clone ID Related Accession (Same CDS sequence)	XM_021228014.1 , XM_005071528.2 , XM_005071528.3 , XM_013114546.1
Accession Version	XM_013114546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012970000.1
CDS	62..5893
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_013114546.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013114546.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA AGATGTCTCC 
CAGGCTTTCC TTGAGGCTGT TGCGGAAGAA AAGCCCCATG CGAAGCCCTT TTTCTCTAAG 
ACCATTCGTG ATCTGGAAGT TGTGGAGGGA AGTGCTGCTA GATTTGACTG CAAGATTGAA 
GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAGGATGACC AGTCAATCAG GGAGTCCCGT 
CACTTCCAGA TAGACTACGA TGAAGATGGG AACTGCTCTC TCATTATTAG CGATGTCTGC 
GGGGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GCCTCGGAGA AGCCACCTGC 
ACTGCGGAGC TCATTGTGGA AACCATGGAG GAAGGGGAAG GGGAAGGGGG AGAGGAAGAG 
GAGGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_013114547.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012970001.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

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CloneID	OMe125132
Clone ID Related Accession (Same CDS sequence)	XM_013114547.1 , XM_013114547.2
Accession Version	XM_013114547.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA TGTCTCCCAG 
GCTTTCCTTG AGGCTGTTGC GGAAGAAAAG CCCCATGCGA AGCCCTTTTT CTCTAAGACC 
ATTCGTGATC TGGAAGTTGT GGAGGGAAGT GCTGCTAGAT TTGACTGCAA GATTGAAGGA 
TACCCAGACC CTGAGGTTGT CTGGTTCAAG GATGACCAGT CAATCAGGGA GTCCCGTCAC 
TTCCAGATAG ACTACGATGA AGATGGGAAC TGCTCTCTCA TTATTAGCGA TGTCTGCGGG 
GATGATGATG CCAAGTACAC CTGCAAGGCT GTCAACAGCC TCGGAGAAGC CACCTGCACT 
GCGGAGCTCA TTGTGGAAAC CATGGAGGAA GGGGAAGGGG AAGGGGGAGA GGAAGAGGAG 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012970001.1
CDS	204..6032
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_013114547.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013114547.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA TGTCTCCCAG 
GCTTTCCTTG AGGCTGTTGC GGAAGAAAAG CCCCATGCGA AGCCCTTTTT CTCTAAGACC 
ATTCGTGATC TGGAAGTTGT GGAGGGAAGT GCTGCTAGAT TTGACTGCAA GATTGAAGGA 
TACCCAGACC CTGAGGTTGT CTGGTTCAAG GATGACCAGT CAATCAGGGA GTCCCGTCAC 
TTCCAGATAG ACTACGATGA AGATGGGAAC TGCTCTCTCA TTATTAGCGA TGTCTGCGGG 
GATGATGATG CCAAGTACAC CTGCAAGGCT GTCAACAGCC TCGGAGAAGC CACCTGCACT 
GCGGAGCTCA TTGTGGAAAC CATGGAGGAA GGGGAAGGGG AAGGGGGAGA GGAAGAGGAG 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_013114547.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012970001.1 Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

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CloneID	OMe125132
Clone ID Related Accession (Same CDS sequence)	XM_013114547.1 , XM_013114547.2
Accession Version	XM_013114547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013114547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA TGTCTCCCAG 
GCTTTCCTTG AGGCTGTTGC GGAAGAAAAG CCCCATGCGA AGCCCTTTTT CTCTAAGACC 
ATTCGTGATC TGGAAGTTGT GGAGGGAAGT GCTGCTAGAT TTGACTGCAA GATTGAAGGA 
TACCCAGACC CTGAGGTTGT CTGGTTCAAG GATGACCAGT CAATCAGGGA GTCCCGTCAC 
TTCCAGATAG ACTACGATGA AGATGGGAAC TGCTCTCTCA TTATTAGCGA TGTCTGCGGG 
GATGATGATG CCAAGTACAC CTGCAAGGCT GTCAACAGCC TCGGAGAAGC CACCTGCACT 
GCGGAGCTCA TTGTGGAAAC CATGGAGGAA GGGGAAGGGG AAGGGGGAGA GGAAGAGGAG 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012970001.1
CDS	231..6059
Translation	MGDVKLVASSHVSKTSHSVDPSRVSSMPLTEAPAFILPPRNLCIKEGATAKFEGRVRGYPEPRVTWHRNGQTITNGGRFLLDCGVRGTFSLVIHTVGEEDNGKYTCEASNGSGARQVTVELTVEGNFMKKHGQPAVSKTSGFPSPVGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRSFVRGTKAASPDIRKEVTNGISKDPETVAESKNCSSPQRSVSSAWATNSHLKSQQEPKLKLCEESPGKALQSSALQKTSSTITLQAMKVQPEARVPGFRAFSPSGEERKSLAAPQPATLPARQSGLGGAVGNKFVARNISTESQRESTFPKFESQPQNQEVTEDQTVKFVCEVSGIPKPEVAWFLEGIPVRRREGIIEIYEDGASHYLCLRRARIRDSGRYSCTASNRLGQVSCSWTLLVERSNLAQTAPSFSSVLKDSVVVEGQDFVLRCSVRGTPVPRITWLLNGQPIKFAHSICEAGVAELHIQDALPEDRGTYTCLAENALGQVSCSARVTVQEKRSEGERENLLLPAPSKPVAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNENEIQESEDFHFEQKGGRHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKSLSKDNGHFELLQNEDVFTLVLKSVQPWHAGQYEILLKNRVGECSCQVSLMLQNSPTRAPPRGREPAGCESLCGGGGVGAHGDGDHDGTLRPGWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEEDLKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPIPEKAPPPKAATPDFRSVLGSKKKSLAENGSNNAEVLNVKAGESPTPVGNTQPGGVLKPIGNAKPAETLKPTGSAKPAETPKPVANAKPAETLKPTANAQPAKSLKPVGNAQPTETLKPMANAKPAETLKPAGKEELKEVKNDVNCKRGHAGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILSQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDARTGENTKVPEMKSRRPKSSLPPLLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESEHIKVESSESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKMWKELTTCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKPWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINADKLESEDVSQAFLEAVAEEKPHAKPFFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE

Target ORF information:

RefSeq Version	XM_013114547.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013114547.2

ORF Insert Sequence:

ATGGGGGACG TGAAACTGGT TGCCTCATCA CACGTGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCAAGGG TCAGCTCCAT GCCCCTCACG GAGGCCCCTG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGAGGGTCCG GGGTTACCCA 
GAGCCCCGGG TGACGTGGCA CAGAAACGGG CAAACCATCA CCAACGGGGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTCAGC CTTGTGATTC ACACTGTTGG TGAGGAGGAC 
AACGGAAAGT ATACCTGTGA AGCCAGCAAC GGCAGTGGTG CCCGCCAGGT GACGGTGGAG 
CTGACTGTGG AAGGGAATTT TATGAAGAAG CATGGTCAGC CTGCTGTTTC CAAAACCTCA 
GGATTTCCAT CTCCTGTGGG GGAGACTCGT CCTAGCATCT GGGGCGAGTG CCCACCAAAG 
TTTGCCACCA AGTTGGGCCG AGCTGTGGTC AAAGAAGGGC AGATGGGGAG ATTCTCCTGC 
AAGATCACTG GTCGGCCTCC ACCACAGGTC ACCTGGCTCA AGGGAAATGT CCCATTGCAG 
CCAAGTGCTC GAGTGTCTAT GTCAGAGAAG AATGGTATGC AGATCCTGGA AATCCATGAG 
GTCACTCAGG ATGACATGGG CACGTACACG TGTATGGTGG TGAATGGGTC AGGGAAAGCC 
TCCATGTCAG CAGAGCTCTC CATCCCAGGT TTGGACAATG CCACTAGGTC ATTTGTAAGA 
GGAACCAAGG CTGCCAGTCC AGACATCAGG AAGGAGGTGA CCAATGGAAT CTCCAAGGAC 
CCAGAAACTG TAGCCGAAAG CAAGAACTGC TCAAGCCCCC AGAGGAGTGT TTCCTCAGCC 
TGGGCCACAA ACAGCCACCT GAAGTCCCAG CAGGAGCCCA AGCTGAAGCT GTGTGAGGAA 
TCTCCTGGAA AGGCCCTGCA GTCCTCAGCC CTGCAGAAGA CTTCCAGCAC CATCACCCTG 
CAGGCTATGA AAGTCCAGCC AGAAGCAAGA GTTCCAGGCT TCAGGGCCTT CTCTCCTTCT 
GGGGAAGAGA GGAAAAGTCT GGCTGCCCCT CAGCCAGCCA CACTCCCTGC CAGACAGAGT 
GGCCTTGGAG GTGCAGTTGG GAACAAATTT GTTGCCAGAA ACATCTCCAC AGAGAGTCAA 
AGGGAGTCAA CATTCCCCAA ATTTGAGAGT CAGCCCCAAA ACCAAGAAGT CACTGAGGAT 
CAAACAGTCA AGTTCGTATG TGAGGTTTCA GGGATACCAA AGCCTGAAGT GGCCTGGTTC 
CTGGAAGGCA TCCCTGTGAG GAGGCGAGAA GGCATCATTG AGATTTATGA AGATGGTGCC 
TCCCATTACC TCTGCCTACG AAGAGCCCGG ATAAGAGACA GTGGGAGATA CAGCTGCACA 
GCATCCAATA GGCTTGGCCA GGTGTCCTGC AGCTGGACCC TCCTGGTGGA ACGGTCAAAC 
TTGGCACAGA CAGCTCCTTC ATTCTCTAGT GTCCTGAAGG ACAGTGTTGT CGTTGAGGGC 
CAGGATTTTG TGCTGAGGTG CTCTGTACGG GGAACCCCAG TACCCCGAAT CACTTGGCTG 
CTCAATGGGC AGCCCATCAA GTTCGCTCAC TCCATCTGCG AGGCTGGTGT GGCTGAGCTC 
CACATCCAGG ATGCCCTGCC AGAGGACCGC GGCACCTACA CCTGCCTGGC TGAGAATGCC 
TTGGGGCAGG TGTCCTGCAG CGCCAGGGTC ACTGTCCAAG AAAAGAGAAG TGAAGGAGAG 
AGGGAAAACC TTCTGCTCCC AGCTCCCAGC AAGCCGGTTG CACCCATCTT CCTGCAAGGC 
CTCTCTGATC TCAAAGTCAT GGATGGAAGC CAGGTCACCA TGACAGTCCA GGTGTCAGGA 
AACCCACCCC CAGAAGTCAT ATGGCTCCAC AATGAGAACG AGATCCAGGA GTCAGAGGAC 
TTCCACTTTG AGCAGAAAGG TGGCCGGCAC AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG 
GACACGGGCA CATACACCTG TGAGGCCTGG AACAGTGCCG GGGAAGTCCG CACCCAGGCC 
ATACTCACTG TGCAAGAGCC TCATGATGGC ACCCAGCCCT GGTTCATCAG CAAGCCACGT 
TCAGTGACAG CCTCCCTGGG CCAGAGTGTC CTCATCTCCT GTGCCATAGC TGGAGACCCC 
TTCCCTACTG TGCACTGGCT CCGCGATGGC AAATCCCTCT CCAAGGATAA TGGCCACTTT 
GAGCTGCTAC AGAATGAGGA CGTGTTCACC TTGGTTCTAA AGAGCGTGCA GCCCTGGCAT 
GCCGGCCAGT ATGAGATCCT CCTCAAGAAC CGGGTTGGCG AGTGCAGTTG CCAGGTGTCA 
CTCATGCTGC AAAACAGTCC CACCAGAGCC CCACCACGGG GGCGGGAGCC TGCCGGCTGT 
GAGAGCCTCT GTGGTGGTGG AGGAGTTGGT GCTCATGGTG ATGGTGACCA TGATGGGACC 
CTGAGGCCTG GCTGGCCAGC AAGAGGGCAG GGTTGGCCAG AGGAGGAAGA CGGAGAAGAC 
GTGAGGGGGC TGCTGAAGAG ACGGGTGGAG ACCAGGCTGC ACACAGAAGA GGCTATCCGC 
CAGCAGGAGG TGGGGCAGCT GGATTTCCGT GACCTCCTGG GAAAGAAGGT GAGCACCAAG 
ACTGTATCAG AAGAAGACCT GAAGGAGATC CCAGCTGAGC AGATGGATTT CCGCGCCAAC 
CTTCAGCGGC AAGTAAAGCC AAAGACAGTG TCCGAGGAAG AGAGGAAGGT GCATAGCCCC 
CAGCAGGTTG ACTTCCGCTC TGTCCTAGCC AAGAAGGGGA CACCTAAGAC CCCCATTCCT 
GAGAAGGCCC CACCTCCAAA AGCTGCCACC CCAGACTTCC GCTCAGTGCT GGGCAGCAAG 
AAGAAGTCAC TGGCAGAAAA TGGCAGCAAC AATGCTGAGG TCTTGAACGT CAAGGCTGGG 
GAAAGCCCCA CGCCTGTAGG CAACACACAG CCAGGTGGGG TCCTGAAACC CATAGGTAAT 
GCCAAGCCGG CTGAGACCCT GAAACCCACA GGTAGTGCTA AGCCTGCTGA GACCCCGAAG 
CCTGTAGCCA ATGCCAAACC TGCTGAGACT CTGAAACCCA CAGCCAATGC ACAGCCTGCT 
AAGTCCCTGA AACCTGTAGG CAATGCTCAG CCTACTGAGA CCCTGAAGCC CATGGCCAAT 
GCCAAGCCTG CCGAGACCCT GAAACCAGCT GGGAAAGAAG AACTCAAGGA GGTTAAGAAT 
GATGTGAACT GTAAGAGAGG GCATGCCGGA GCCACAGGCA ATGAAAAGCG ACCCGAGAGC 
CAAGGCTCGG CCCCAGTCTT CAAGGAGAAG CTACAAGATG TCCATGTGGC AGAAGGGGAG 
AAGCTGCTAC TCCAGTGCCA GGTGACCTCT GACCCACCGG CCACTGTCAC CTGGACACTG 
AATGGAAAGA CACTCAAGAC CACAAAATTC ATCATCCTCT CCCAAGAAGG CTCACTCTTC 
TCTGTCTCCA TCGAGAAAGC ACTGCCTGAG GACAGGGGCT TGTACAAGTG TGTAGCCAAG 
AACAGCGCTG GCCAGGCAGA GTGTTCCTGC CAAGTCACTG TGGATGATGC TCGGACCGGT 
GAGAACACCA AGGTCCCGGA GATGAAATCC CGGAGACCCA AGAGCTCTCT TCCCCCTCTG 
CTAGGAACAG AGAGTGATGC AACTGTAAAA AAGAAACCTG CACCCAAGAC ACCTACAAAA 
GCAGCTATGC CCCCTCAAAT CATCCAGTTC CCCGAGGACC AGAAGGTGCG CGCAGGCGAG 
CCTGTGGAGC TGTTTGGAAA AGTGACTGGT ACCCAGCCCA TCACCTGCAC ATGGATGAAG 
TTCCGAAAGC AGATCCAGGA GAGCGAGCAC ATCAAGGTGG AGAGCAGTGA GAGCGGCAGC 
AAGCTCACCA TCCTGGCAGC ACGCCAGGAG CACTGTGGCT GCTACACATT GGTGGTGGAG 
AACAAGCTGG GCAGCAGGCA GGCCCAAGTC AACCTCACCG TCGTGGATAA GCCCGACCCC 
CCAGCTGGCA CACCTTGTGC ATCTGACATA CGGAGTTCCT CGCTGACCCT GTCCTGGTAC 
GGCTCTTCCT ATGATGGAGG CAGTGCTGTA CAGTCCTACA ATGTTGAGAT CTGGGACACG 
GAGGACAAGA TGTGGAAAGA ACTAACCACA TGCCGCAGCA CCTCTTTTAA TGTCCAAGAC 
CTGCTGCCTG ACCGCGAGTA TAAATTCCGA GTACGTGCGG TCAACGTCTA TGGAACCAGC 
GAGCCGAGCC AGGAGTCTGA GCTCACAGCT GTGGGAGAGA AACCTGAGGA GCCAAAGGAT 
GAAGTGGAAG TGTCAGATGA TGACGAAAAG GAGCCTGAAG TCGATTATCG GACAGTGACA 
GTTAACACTG AACAGAAAGT ATCTGATGTG TATGACATTG AAGAGCGACT AGGATCTGGG 
AAGTTTGGAC AAGTCTTCCG ACTTGTGGAA AAGAAAACTG GAAAACCCTG GGCAGGGAAG 
TTTTTCAAGG CCTATTCTGC CAAGGAAAAA GAGAACATCC GGCAGGAGAT CAGCATCATG 
AACTGTCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGATG CCTTCGAAGA AAAGGCCAAC 
ATCGTCATGG TTCTGGAGAT CGTGTCAGGG GGCGAGCTGT TTGAGCGCAT CATTGACGAG 
GACTTCGAGC TGACGGAGCG AGAGTGCATC AAGTACATGA GGCAGATTTC AGAAGGGGTG 
GAGTACATCC ACAAGCAGGG CATTGTGCAC CTGGACCTCA AGCCCGAGAA CATCATGTGT 
GTCAACAAGA CAGGCACCAG GATCAAGCTC ATTGACTTCG GTCTGGCACG AAGACTCGAG 
AATGCTGGGT CTCTGAAGGT CCTCTTCGGA ACCCCGGAGT TTGTGGCTCC TGAAGTGATC 
AACTATGAAC CCATCGGCTA CGCTACAGAC ATGTGGAGCA TTGGGGTCAT CTGCTATATT 
CTGGTCAGCG GACTGTCTCC CTTCATGGGT GACAATGATA ATGAGACCTT AGCCAATGTC 
ACTTCTGCCA CCTGGGACTT TGATGATGAG GCGTTCGATG AAATCTCCGA CGATGCCAAG 
GACTTCATCA GTAACTTGCT GAAGAAAGAT ATGAAAAACC GTCTGGACTG CACGCAGTGC 
CTTCAGCACC CATGGCTGAT GAAAGACACC AAGAACATGG AGGCCAAGAA GCTGTCCAAG 
GACCGCATGA AGAAGTACAT GGCAAGGAGG AAGTGGCAGA AAACGGGCAA TGCTGTGAGA 
GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA 
ACGGGGTCAC CAACCAGCCC AATCAATGCA GACAAACTAG AGTCCGAAGA TGTCTCCCAG 
GCTTTCCTTG AGGCTGTTGC GGAAGAAAAG CCCCATGCGA AGCCCTTTTT CTCTAAGACC 
ATTCGTGATC TGGAAGTTGT GGAGGGAAGT GCTGCTAGAT TTGACTGCAA GATTGAAGGA 
TACCCAGACC CTGAGGTTGT CTGGTTCAAG GATGACCAGT CAATCAGGGA GTCCCGTCAC 
TTCCAGATAG ACTACGATGA AGATGGGAAC TGCTCTCTCA TTATTAGCGA TGTCTGCGGG 
GATGATGATG CCAAGTACAC CTGCAAGGCT GTCAACAGCC TCGGAGAAGC CACCTGCACT 
GCGGAGCTCA TTGTGGAAAC CATGGAGGAA GGGGAAGGGG AAGGGGGAGA GGAAGAGGAG 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Mylk cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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