Nf1 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nf1
Entrez Gene ID	101826542
Full Name	neurofibromin 1
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005077086.2	XP_005077143.1	neurofibromin
XM_021231139.1	XP_021086798.1	neurofibromin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters.
McCann KE, Sinkiewicz DM, Norvelle A, Huhman KL
Scientific reports740472(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe130651	XM_005077086.2 Latest version!	Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMe251160	XM_021231139.1 Latest version!	Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nf1 ( XM_005077086.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005077143.1 Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.

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CloneID	OMe130651
Clone ID Related Accession (Same CDS sequence)	XM_005077086.2
Accession Version	XM_005077086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	neurofibromin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005077086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAAAATACA CATACCAAAG TCAGCACCGA GCATAACAAG 
GAATGTCTGA TCAACATTTC CAAATACAAG TTTTCTTTGG TCATAAGTGG CCTCACTACC 
ATCTTGAAGA ATGTTAATAA TATGCGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTTTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTAC TGCCAGAAAT CTGCCATTTT 
CTTCACACCT GTCGTGAAGG AAACCAACAT GCGGCTGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG 
TTACAGGAGT TGACTGTTTG TTCAGAAGAC AATGTTGATG TCCATGATAT AGAATTGTTA 
CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT CGCACAGTTA GCAGTTATTA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TAGAAAACTA TCCGGATGAA TTTACAAAGC TGTACCAAAT TCCACAGACC 
GATATGGCTG AGTGTGCAGA AAAGCTGTTT GACTTGGTGG ACGGTTTTGC TGAAAGCACC 
AAACGTAAAG CCGCAGTTTG GCCACTGCAA ATCATTCTCC TCATTTTGTG TCCAGAAATA 
ATCCAGGATA TATCCAAGGA TGTAGTTGAT GACAACAACA TAAATAAGAA GCTGTTTCTG 
GATAGTTTAC GAAAAGCACT TGCTGGCCAT GGAGGAAGCA GGCAACTGAC AGAGAGTGCT 
GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTATA TCAACTGGGA AGATAACTCT 
GTCATTTTCC TACTAGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAGCACT TTAAGATCTG CCTGGCGCAG 
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAACTCAC TCCACCGCAT CATCACCAAT 
TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGA 
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAGGGCT GTGGAGCGCA CCCAGCAATA 
CGAATGGCAC CAAGTCTTAC ATTTAAAGAA AAGGTAACAA GTCTTAAATT TAAAGAAAAG 
CCTACAGACC TGGAAACAAG GAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT 
CACGCAGACC CAAAGCTTTT ACTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAAGGC 
AGTACGGCAG AATTAATCAC AGGGCTTGTC CAACTGGTCC CTCAGTCACA TATGCCAGAG 
ATTGCTCAGG AAGCGATGGA GGCCTTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTA 
TGGAACCCTG ATGCTCCTGT AGAGACATTT TGGGAAATTA GTTCACAAAT GCTTTTTTAC 
ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA 
CGGGAAATTC TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC GGATAGAAGT 
TCCTGTCACT TTCTCTTCCT TTGTGGAGTA GGATGTGATG TTCCTGCTAG TGGAAATACC 
ACTCAGATGT CAATGGATCA TGATGAGTTA TTAAGGACCT GCACTCCTGG AGCATCTTTG 
CGGAAAGGAA AAGGAAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCA 
ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCTTTGT ACATGTTTCT GTGGAGTCCT 
GACACTGAAG CTGTTCTGGT TGCTATGTCT TGTTTCCGCC ACCTGTGTGA AGAAGCAGAT 
ATCAGGTGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC 
ATGGAATTTG CCTCTGTCAG CAGTATGATG TCAACAGGAA GAGCAGCCCT TCAGAAGAGA 
GTGATGGCCC TGCTGAGGCG CATTGAGCAC CCCACTGCAG GAAACACTGA GGCTTGGGAA 
GATACACATG CAAAATGGGA ACAGGCTACA AAACTAATCC TTAACTATCC GAAAGCCAAA 
ATGGAAGATG GCCAGGCTGC AGAAAGCCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC 
CATGTCAGTG GAGGTGGATC CATAGACTTG TCTGACACAG ACTCGCTGCA GGAATGGATC 
AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT 
GGCTTAGCGA CCTACAGCCC ACCTATGGGC CCTGTCAGTG AGCGCAAAGG GTCTATGATT 
TCCGTAATGT CTTCTGAAGG GAATGTTGAC ACACCTGTCA GCAGATTTAT GGACCGGCTT 
CTGTCTTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGAAC CAATGTTAAG 
GACCTGGTGG GCCTGGAGTT GAGTCCTGCT CTGTATCCAA TGTTGTTTAA CAAACTGAAG 
AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TATTGACTGA TAGCAATACT 
CAGTTTGTAG AGCAAACTAT AGCCATAATG AAGAACCTGC TGGATAATCA TACTGAAGGC 
AGCTCTGAGC ATCTGGGACA AGCTAGCATT GAAACAATGA TGTTAAATCT GGTTAGATAC 
GTTCGTGTAC TTGGGAATGT GGTCCATGCA ATTCAAATAA AAACAAAACT GTGCCAGTTA 
GTTGAAGTAA TGATGGCAAG AAGAGATGAT CTCTCATTTT GTCAGGAGAT GAAATTTAGG 
AATAAAATGG TAGAATACCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT 
GATGATGTAA AGTGTCTTAC AAGAGATTTG GACCAAGCAA GCATGGAAGC AGTAGTTTCA 
CTCCTCGCTG GTCTTCCACT ACAGCCTGAA GAAGGAGATG GGGTGGAACT AATGGAGGCC 
AAATCACAGT TATTTCTTAA GTACTTCACA CTATTTATGA ACCTTTTGAA TGACTGTAGT 
GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGAAAAC GTGGCATGTC TCGGAGGCTG 
GCATCCTTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC 
AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTCCAGAC AAGAGCTACG 
TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTTGACAC ACTTGCTGAA 
ACGGTGTTGG CTGATCGGTT TGAGAGACTC GTGGAACTGG TCACAATGAT GGGAGACCAA 
GGAGAGCTCC CTATAGCTAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG 
CTGGCTCGTG TTCTGGTTAC TCTGTTTGAT TCTCGGCACT TACTCTACCA GCTGCTTTGG 
AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC 
AGCTTGGCCA GTAAGATAAT GACATTCTGC TTTAAGGTTT ATGGTGCTAC TTACCTACAA 
AAGCTCTTGG ATCCTTTATT ACGTATTGTG ATCACATCCT CAGATTGGCA ACATGTTAGC 
TTTGAAGTGG ATCCTACCAG GTTAGAATCT ACTGAGAGCC TTGAGGAAAA CCAGAGGAAC 
CTCCTTCAAA TGACAGAGAA GTTCTTCCAT GCCATCATCA GTTCCTCCTC AGAATTCCCC 
TCCCAGCTTC GAAGTGTCTG CCATTGCTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT 
AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA ACGTTTCCCT 
CAGAACAGCA TCGGTGCGGT AGGAAGTGCC ATGTTCCTCA GATTTATCAA TCCTGCCATT 
GTCTCGCCAT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT CGAAAGGGGC 
TTGAAGTTAA TGTCAAAGAT ACTTCAGAGC ATAGCCAATC ATGTCCTGTT CACAAAAGAA 
GAACATATGA GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGATTT 
TTCCTTGATA TAGCATCAGA TTGTCCCACA AGTGATGCTG TAAATCATAG TCTTTCCTTC 
ATTAGTGATG GCAATGTACT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT 
GGCCAGTATC TTTCCAGCAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG 
ATGGCCATGC TTCTTGCATA CCTGGGTCCT CCAGAGCATA AGCCTGTGGC AGATACACAC 
TGGTCCAGCC TTAACCTTAC CAGTTCCAAG TTTGAGGAGT TTATGACCAG GCATCAGGTA 
CATGAGAAAG AAGAGTTCAA GGCTTTGAAA ACTTTAAGTA TCTTCTACCA AGCTGGCACT 
TCTAAAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGAA GGTTCAAAAC TGGCCAAATC 
AATGGTGATT TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA 
TATGAAATTG TAGTGGACCT TACCCATACT GGACCTAGCA ATCGCTTTAA AACAGATTTT 
CTCTCTAAGT GGTTTGTTGT TTTTCCTGGC TTTGCTTACG ACAATGTCTC TGCAGTTTAT 
ATTTATAACT GTAACTCCTG GGTCAGAGAG TACACCAAGT ACCATGAACG GCTACTGACT 
GGCCTCAAGG GCAGCAAACG GCTTATTTTC ATAGACTGTC CTGGGAAGCT GGCTGAGCAC 
ATAGAGCATG AACAACAGAA ACTCCCTGCT GCCACATTGG CTCTGGAGGA GGACCTTAAG 
GTGTTCCACA ATGCTCTGAA GCTAGCTCAC AAAGATACCA AAGTCTCTAT TAAGGTTGGT 
TCTACTGCTG TTCAAGTAAC CTCAGCAGAG AGAACAAAAG TTTTGGGGCA ATCTGTGTTT 
TTAAATGACA TCTACTATGC TTCTGAAATC GAAGAGATCT GCCTTGTGGA TGAGAACCAG 
TTCACCTTAA CCATTGCAAA CCAAGGCACA CCACTTACCT TCATGCACCA GGAGTGTGAA 
GCCATTGTCC AGTCTATTAT TCACATCAGG ACCCGCTGGG AGCTATCACA GCCTGACTCT 
ATCCCCCAAC ATACCAAGAT TCGACCAAAA GATGTTCCTG GGACACTTCT TAATATTGCA 
TTACTTAATT TAGGCAGTTC AGACCCTAGC CTACGGTCTG CTGCCTATAA TCTTCTGTGT 
GCCTTAACCT GTACCTTTAA TTTAAAAATT GAAGGCCAGT TACTAGAGAC ATCAGGGTTA 
TGCATCCCTG CCAACAATAC CCTCTTTATT GTCTCAATCA GTAAGACACT GGCCGCCAAT 
GAGCCACATC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAAC 
CTCTTAAAAG GGTACAGGCA TCCTTCACCT GCCATTGTTG CAAGAACAGT CAGAATTTTG 
CATACACTAC TAACTCTTGT TAACAAACAT AGAAATTGTG ACAAATTTGA GGTGAATACA 
CAGAGTGTGG CTTACTTAGC AGCTCTACTC ACAGTGTCTG AAGAGGTTCG AAGTCGCTGT 
AGCCTCAAGC ATAGAAAGTC CCTTCTTCTA ACTGATATTT CGATGGAAAA TGTTCCTATG 
GATACATACC CCATTCTTCA TGGTGATCCT AGCTATAGGA CATTAAAGGA GAACCAGCCA 
TGGTCCTCTC CCAAGGGTTC AGAAGGATAC CTTGCAGCTA CCTACCCATC TGTAGGCCAG 
ACCAGTCCCC GAGCCAGGAA ATCCATGAGC TTGGACATGG GACAGCCTTC TCAAGCCAAC 
ACAAAGAAAT TACTTGGAAC AAGAAAAAGT TTTGATCACT TGATATCAGA CACAAAGGCT 
CCCAAAAGAC AAGAAATGGA ATCAGGGATC ACAACACCCC CCAAAATGAG AAGAGTGGCA 
GAAACTGATT ATGAAATGGA AACCCAAAGG ATTTCCTCAC AGCAGCACCC ACATTTACGG 
AAAGTCTCTG TGTCTGAATC AAATGTTCTT TTGGATGAAG AAGTACTTAC TGATCCAAAG 
ATACAAGCAC TGCTTCTTAC TGTTCTGGCT ACACTGGTAA AATATACTAC AGATGAATTT 
GATCAACGAA TTCTTTATGA ATACCTAGCA GAGGCCAGTG TTGTGTTTCC CAAAGTGTTT 
CCTGTTGTGC ACAATTTGTT GGACTCTAAG ATCAATACCC TCTTGTCATT GTGCCAAGAT 
CCAAATTTGT TAAATCCAAT CCATGGAATT GTGCAGAGTG TGGTGTACCA TGAGGAATCC 
CCTCCACAGT ACCAAACATC TTACCTGCAA AGTTTTGGTT TCAATGGCTT GTGGCGTTTT 
GCAGGACCCT TTTCAAAGCA AACACAAATC CCAGACTATG CTGAGCTTAT TGTTAAGTTT 
CTTGATGCCT TGATTGACAC GTACTTGCCT GGAATTGATG AAGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACATCTCC TTACCCTCCT GCACTGCAGA GCCAGCTTAG TATCACTGCC 
AACCTTAACC TCTCTAATTC CATGACCTCA CTTGCAACTT CCCAGCATTC CCCAGGAATC 
GACAAGGAGA ACGTTGAACT CTCCCCCACC ACTGGCCACT GTAACAGTGG ACGAACTCGC 
CATGGATCCG CAAGCCAGGT GCAGAAGCAA AGAAGCGCTG GCAGTTTCAA ACGTAATAGC 
ATTAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005077143.1
CDS	361..7518
Translation	MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDDNNINKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLCGVGCDVPASGNTTQMSMDHDELLRTCTPGASLRKGKGNSSMDSTAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSSMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSSSGLATYSPPMGPVSERKGSMISVMSSEGNVDTPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDSNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNVVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLESTESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMAMLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSNLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPILHGDPSYRTLKENQPWSSPKGSEGYLAATYPSVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_005077086.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005077086.2

ORF Insert Sequence:

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAAAATACA CATACCAAAG TCAGCACCGA GCATAACAAG 
GAATGTCTGA TCAACATTTC CAAATACAAG TTTTCTTTGG TCATAAGTGG CCTCACTACC 
ATCTTGAAGA ATGTTAATAA TATGCGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTTTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTAC TGCCAGAAAT CTGCCATTTT 
CTTCACACCT GTCGTGAAGG AAACCAACAT GCGGCTGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG 
TTACAGGAGT TGACTGTTTG TTCAGAAGAC AATGTTGATG TCCATGATAT AGAATTGTTA 
CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT CGCACAGTTA GCAGTTATTA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TAGAAAACTA TCCGGATGAA TTTACAAAGC TGTACCAAAT TCCACAGACC 
GATATGGCTG AGTGTGCAGA AAAGCTGTTT GACTTGGTGG ACGGTTTTGC TGAAAGCACC 
AAACGTAAAG CCGCAGTTTG GCCACTGCAA ATCATTCTCC TCATTTTGTG TCCAGAAATA 
ATCCAGGATA TATCCAAGGA TGTAGTTGAT GACAACAACA TAAATAAGAA GCTGTTTCTG 
GATAGTTTAC GAAAAGCACT TGCTGGCCAT GGAGGAAGCA GGCAACTGAC AGAGAGTGCT 
GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTATA TCAACTGGGA AGATAACTCT 
GTCATTTTCC TACTAGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAGCACT TTAAGATCTG CCTGGCGCAG 
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAACTCAC TCCACCGCAT CATCACCAAT 
TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGA 
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAGGGCT GTGGAGCGCA CCCAGCAATA 
CGAATGGCAC CAAGTCTTAC ATTTAAAGAA AAGGTAACAA GTCTTAAATT TAAAGAAAAG 
CCTACAGACC TGGAAACAAG GAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT 
CACGCAGACC CAAAGCTTTT ACTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAAGGC 
AGTACGGCAG AATTAATCAC AGGGCTTGTC CAACTGGTCC CTCAGTCACA TATGCCAGAG 
ATTGCTCAGG AAGCGATGGA GGCCTTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTA 
TGGAACCCTG ATGCTCCTGT AGAGACATTT TGGGAAATTA GTTCACAAAT GCTTTTTTAC 
ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA 
CGGGAAATTC TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC GGATAGAAGT 
TCCTGTCACT TTCTCTTCCT TTGTGGAGTA GGATGTGATG TTCCTGCTAG TGGAAATACC 
ACTCAGATGT CAATGGATCA TGATGAGTTA TTAAGGACCT GCACTCCTGG AGCATCTTTG 
CGGAAAGGAA AAGGAAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCA 
ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCTTTGT ACATGTTTCT GTGGAGTCCT 
GACACTGAAG CTGTTCTGGT TGCTATGTCT TGTTTCCGCC ACCTGTGTGA AGAAGCAGAT 
ATCAGGTGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC 
ATGGAATTTG CCTCTGTCAG CAGTATGATG TCAACAGGAA GAGCAGCCCT TCAGAAGAGA 
GTGATGGCCC TGCTGAGGCG CATTGAGCAC CCCACTGCAG GAAACACTGA GGCTTGGGAA 
GATACACATG CAAAATGGGA ACAGGCTACA AAACTAATCC TTAACTATCC GAAAGCCAAA 
ATGGAAGATG GCCAGGCTGC AGAAAGCCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC 
CATGTCAGTG GAGGTGGATC CATAGACTTG TCTGACACAG ACTCGCTGCA GGAATGGATC 
AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT 
GGCTTAGCGA CCTACAGCCC ACCTATGGGC CCTGTCAGTG AGCGCAAAGG GTCTATGATT 
TCCGTAATGT CTTCTGAAGG GAATGTTGAC ACACCTGTCA GCAGATTTAT GGACCGGCTT 
CTGTCTTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGAAC CAATGTTAAG 
GACCTGGTGG GCCTGGAGTT GAGTCCTGCT CTGTATCCAA TGTTGTTTAA CAAACTGAAG 
AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TATTGACTGA TAGCAATACT 
CAGTTTGTAG AGCAAACTAT AGCCATAATG AAGAACCTGC TGGATAATCA TACTGAAGGC 
AGCTCTGAGC ATCTGGGACA AGCTAGCATT GAAACAATGA TGTTAAATCT GGTTAGATAC 
GTTCGTGTAC TTGGGAATGT GGTCCATGCA ATTCAAATAA AAACAAAACT GTGCCAGTTA 
GTTGAAGTAA TGATGGCAAG AAGAGATGAT CTCTCATTTT GTCAGGAGAT GAAATTTAGG 
AATAAAATGG TAGAATACCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT 
GATGATGTAA AGTGTCTTAC AAGAGATTTG GACCAAGCAA GCATGGAAGC AGTAGTTTCA 
CTCCTCGCTG GTCTTCCACT ACAGCCTGAA GAAGGAGATG GGGTGGAACT AATGGAGGCC 
AAATCACAGT TATTTCTTAA GTACTTCACA CTATTTATGA ACCTTTTGAA TGACTGTAGT 
GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGAAAAC GTGGCATGTC TCGGAGGCTG 
GCATCCTTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC 
AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTCCAGAC AAGAGCTACG 
TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTTGACAC ACTTGCTGAA 
ACGGTGTTGG CTGATCGGTT TGAGAGACTC GTGGAACTGG TCACAATGAT GGGAGACCAA 
GGAGAGCTCC CTATAGCTAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG 
CTGGCTCGTG TTCTGGTTAC TCTGTTTGAT TCTCGGCACT TACTCTACCA GCTGCTTTGG 
AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC 
AGCTTGGCCA GTAAGATAAT GACATTCTGC TTTAAGGTTT ATGGTGCTAC TTACCTACAA 
AAGCTCTTGG ATCCTTTATT ACGTATTGTG ATCACATCCT CAGATTGGCA ACATGTTAGC 
TTTGAAGTGG ATCCTACCAG GTTAGAATCT ACTGAGAGCC TTGAGGAAAA CCAGAGGAAC 
CTCCTTCAAA TGACAGAGAA GTTCTTCCAT GCCATCATCA GTTCCTCCTC AGAATTCCCC 
TCCCAGCTTC GAAGTGTCTG CCATTGCTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT 
AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA ACGTTTCCCT 
CAGAACAGCA TCGGTGCGGT AGGAAGTGCC ATGTTCCTCA GATTTATCAA TCCTGCCATT 
GTCTCGCCAT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT CGAAAGGGGC 
TTGAAGTTAA TGTCAAAGAT ACTTCAGAGC ATAGCCAATC ATGTCCTGTT CACAAAAGAA 
GAACATATGA GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGATTT 
TTCCTTGATA TAGCATCAGA TTGTCCCACA AGTGATGCTG TAAATCATAG TCTTTCCTTC 
ATTAGTGATG GCAATGTACT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT 
GGCCAGTATC TTTCCAGCAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG 
ATGGCCATGC TTCTTGCATA CCTGGGTCCT CCAGAGCATA AGCCTGTGGC AGATACACAC 
TGGTCCAGCC TTAACCTTAC CAGTTCCAAG TTTGAGGAGT TTATGACCAG GCATCAGGTA 
CATGAGAAAG AAGAGTTCAA GGCTTTGAAA ACTTTAAGTA TCTTCTACCA AGCTGGCACT 
TCTAAAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGAA GGTTCAAAAC TGGCCAAATC 
AATGGTGATT TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA 
TATGAAATTG TAGTGGACCT TACCCATACT GGACCTAGCA ATCGCTTTAA AACAGATTTT 
CTCTCTAAGT GGTTTGTTGT TTTTCCTGGC TTTGCTTACG ACAATGTCTC TGCAGTTTAT 
ATTTATAACT GTAACTCCTG GGTCAGAGAG TACACCAAGT ACCATGAACG GCTACTGACT 
GGCCTCAAGG GCAGCAAACG GCTTATTTTC ATAGACTGTC CTGGGAAGCT GGCTGAGCAC 
ATAGAGCATG AACAACAGAA ACTCCCTGCT GCCACATTGG CTCTGGAGGA GGACCTTAAG 
GTGTTCCACA ATGCTCTGAA GCTAGCTCAC AAAGATACCA AAGTCTCTAT TAAGGTTGGT 
TCTACTGCTG TTCAAGTAAC CTCAGCAGAG AGAACAAAAG TTTTGGGGCA ATCTGTGTTT 
TTAAATGACA TCTACTATGC TTCTGAAATC GAAGAGATCT GCCTTGTGGA TGAGAACCAG 
TTCACCTTAA CCATTGCAAA CCAAGGCACA CCACTTACCT TCATGCACCA GGAGTGTGAA 
GCCATTGTCC AGTCTATTAT TCACATCAGG ACCCGCTGGG AGCTATCACA GCCTGACTCT 
ATCCCCCAAC ATACCAAGAT TCGACCAAAA GATGTTCCTG GGACACTTCT TAATATTGCA 
TTACTTAATT TAGGCAGTTC AGACCCTAGC CTACGGTCTG CTGCCTATAA TCTTCTGTGT 
GCCTTAACCT GTACCTTTAA TTTAAAAATT GAAGGCCAGT TACTAGAGAC ATCAGGGTTA 
TGCATCCCTG CCAACAATAC CCTCTTTATT GTCTCAATCA GTAAGACACT GGCCGCCAAT 
GAGCCACATC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAAC 
CTCTTAAAAG GGTACAGGCA TCCTTCACCT GCCATTGTTG CAAGAACAGT CAGAATTTTG 
CATACACTAC TAACTCTTGT TAACAAACAT AGAAATTGTG ACAAATTTGA GGTGAATACA 
CAGAGTGTGG CTTACTTAGC AGCTCTACTC ACAGTGTCTG AAGAGGTTCG AAGTCGCTGT 
AGCCTCAAGC ATAGAAAGTC CCTTCTTCTA ACTGATATTT CGATGGAAAA TGTTCCTATG 
GATACATACC CCATTCTTCA TGGTGATCCT AGCTATAGGA CATTAAAGGA GAACCAGCCA 
TGGTCCTCTC CCAAGGGTTC AGAAGGATAC CTTGCAGCTA CCTACCCATC TGTAGGCCAG 
ACCAGTCCCC GAGCCAGGAA ATCCATGAGC TTGGACATGG GACAGCCTTC TCAAGCCAAC 
ACAAAGAAAT TACTTGGAAC AAGAAAAAGT TTTGATCACT TGATATCAGA CACAAAGGCT 
CCCAAAAGAC AAGAAATGGA ATCAGGGATC ACAACACCCC CCAAAATGAG AAGAGTGGCA 
GAAACTGATT ATGAAATGGA AACCCAAAGG ATTTCCTCAC AGCAGCACCC ACATTTACGG 
AAAGTCTCTG TGTCTGAATC AAATGTTCTT TTGGATGAAG AAGTACTTAC TGATCCAAAG 
ATACAAGCAC TGCTTCTTAC TGTTCTGGCT ACACTGGTAA AATATACTAC AGATGAATTT 
GATCAACGAA TTCTTTATGA ATACCTAGCA GAGGCCAGTG TTGTGTTTCC CAAAGTGTTT 
CCTGTTGTGC ACAATTTGTT GGACTCTAAG ATCAATACCC TCTTGTCATT GTGCCAAGAT 
CCAAATTTGT TAAATCCAAT CCATGGAATT GTGCAGAGTG TGGTGTACCA TGAGGAATCC 
CCTCCACAGT ACCAAACATC TTACCTGCAA AGTTTTGGTT TCAATGGCTT GTGGCGTTTT 
GCAGGACCCT TTTCAAAGCA AACACAAATC CCAGACTATG CTGAGCTTAT TGTTAAGTTT 
CTTGATGCCT TGATTGACAC GTACTTGCCT GGAATTGATG AAGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACATCTCC TTACCCTCCT GCACTGCAGA GCCAGCTTAG TATCACTGCC 
AACCTTAACC TCTCTAATTC CATGACCTCA CTTGCAACTT CCCAGCATTC CCCAGGAATC 
GACAAGGAGA ACGTTGAACT CTCCCCCACC ACTGGCCACT GTAACAGTGG ACGAACTCGC 
CATGGATCCG CAAGCCAGGT GCAGAAGCAA AGAAGCGCTG GCAGTTTCAA ACGTAATAGC 
ATTAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf1 ( XM_021231139.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021086798.1 Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.

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CloneID	OMe251160
Clone ID Related Accession (Same CDS sequence)	XM_021231139.1
Accession Version	XM_021231139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	neurofibromin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801671.1) and transcript sequence (GEMX01090599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1377 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAAAATACA CATACCAAAG TCAGCACCGA GCATAACAAG 
GAATGTCTGA TCAACATTTC CAAATACAAG TTTTCTTTGG TCATAAGTGG CCTCACTACC 
ATCTTGAAGA ATGTTAATAA TATGCGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTTTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTAC TGCCAGAAAT CTGCCATTTT 
CTTCACACCT GTCGTGAAGG AAACCAACAT GCGGCTGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG 
TTACAGGAGT TGACTGTTTG TTCAGAAGAC AATGTTGATG TCCATGATAT AGAATTGTTA 
CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT CGCACAGTTA GCAGTTATTA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TAGAAAACTA TCCGGATGAA TTTACAAAGC TGTACCAAAT TCCACAGACC 
GATATGGCTG AGTGTGCAGA AAAGCTGTTT GACTTGGTGG ACGGTTTTGC TGAAAGCACC 
AAACGTAAAG CCGCAGTTTG GCCACTGCAA ATCATTCTCC TCATTTTGTG TCCAGAAATA 
ATCCAGGATA TATCCAAGGA TGTAGTTGAT GACAACAACA TAAATAAGAA GCTGTTTCTG 
GATAGTTTAC GAAAAGCACT TGCTGGCCAT GGAGGAAGCA GGCAACTGAC AGAGAGTGCT 
GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTATA TCAACTGGGA AGATAACTCT 
GTCATTTTCC TACTAGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAGCACT TTAAGATCTG CCTGGCGCAG 
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAACTCAC TCCACCGCAT CATCACCAAT 
AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT 
GCTGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA 
GTGCAGGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTTAC ATTTAAAGAA 
AAGGTAACAA GTCTTAAATT TAAAGAAAAG CCTACAGACC TGGAAACAAG GAGCTATAAG 
TATCTTCTCT TGTCCATGGT GAAACTAATT CACGCAGACC CAAAGCTTTT ACTTTGTAAT 
CCAAGAAAAC AGGGCCCTGA AACTCAAGGC AGTACGGCAG AATTAATCAC AGGGCTTGTC 
CAACTGGTCC CTCAGTCACA TATGCCAGAG ATTGCTCAGG AAGCGATGGA GGCCTTGCTG 
GTTCTTCATC AGTTAGATAG CATTGATTTA TGGAACCCTG ATGCTCCTGT AGAGACATTT 
TGGGAAATTA GTTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG 
CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG GAATAAATTT 
CTTCTTAAAA ATAAGCAGGC GGATAGAAGT TCCTGTCACT TTCTCTTCCT TTGTGGAGTA 
GGATGTGATG TTCCTGCTAG TGGAAATACC ACTCAGATGT CAATGGATCA TGATGAGTTA 
TTAAGGACCT GCACTCCTGG AGCATCTTTG CGGAAAGGAA AAGGAAATTC CTCCATGGAT 
AGCACAGCAG GGTGCAGTGG AACCCCACCA ATATGCCGAC AAGCCCAGAC CAAGCTAGAA 
GTGGCTTTGT ACATGTTTCT GTGGAGTCCT GACACTGAAG CTGTTCTGGT TGCTATGTCT 
TGTTTCCGCC ACCTGTGTGA AGAAGCAGAT ATCAGGTGTG GGGTAGATGA AGTGTCAGTA 
CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCTGTCAG CAGTATGATG 
TCAACAGGAA GAGCAGCCCT TCAGAAGAGA GTGATGGCCC TGCTGAGGCG CATTGAGCAC 
CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAATGGGA ACAGGCTACA 
AAACTAATCC TTAACTATCC GAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGCCTT 
CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGTGGATC CATAGACTTG 
TCTGACACAG ACTCGCTGCA GGAATGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT 
GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCTTAGCGA CCTACAGCCC ACCTATGGGC 
CCTGTCAGTG AGCGCAAAGG GTCTATGATT TCCGTAATGT CTTCTGAAGG GAATGTTGAC 
ACACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCTTTAA TGGTGTGTAA CCACGAGAAA 
GTGGGGCTTC AGATACGAAC CAATGTTAAG GACCTGGTGG GCCTGGAGTT GAGTCCTGCT 
CTGTATCCAA TGTTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA 
GGACAGGTAT TATTGACTGA TAGCAATACT CAGTTTGTAG AGCAAACTAT AGCCATAATG 
AAGAACCTGC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCTAGCATT 
GAAACAATGA TGTTAAATCT GGTTAGATAC GTTCGTGTAC TTGGGAATGT GGTCCATGCA 
ATTCAAATAA AAACAAAACT GTGCCAGTTA GTTGAAGTAA TGATGGCAAG AAGAGATGAT 
CTCTCATTTT GTCAGGAGAT GAAATTTAGG AATAAAATGG TAGAATACCT AACAGACTGG 
GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AGTGTCTTAC AAGAGATTTG 
GACCAAGCAA GCATGGAAGC AGTAGTTTCA CTCCTCGCTG GTCTTCCACT ACAGCCTGAA 
GAAGGAGATG GGGTGGAACT AATGGAGGCC AAATCACAGT TATTTCTTAA GTACTTCACA 
CTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT 
GGCAGAAAAC GTGGCATGTC TCGGAGGCTG GCATCCTTGA GGCACTGTAC AGTGCTTGCA 
ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT 
TATCACAAGG ATCTCCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA 
CAAGGAACAG AATTTGACAC ACTTGCTGAA ACGGTGTTGG CTGATCGGTT TGAGAGACTC 
GTGGAACTGG TCACAATGAT GGGAGACCAA GGAGAGCTCC CTATAGCTAT GGCTCTGGCC 
AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGTG TTCTGGTTAC TCTGTTTGAT 
TCTCGGCACT TACTCTACCA GCTGCTTTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA 
GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC 
TTTAAGGTTT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ATCCTTTATT ACGTATTGTG 
ATCACATCCT CAGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAATCT 
ACTGAGAGCC TTGAGGAAAA CCAGAGGAAC CTCCTTCAAA TGACAGAGAA GTTCTTCCAT 
GCCATCATCA GTTCCTCCTC AGAATTCCCC TCCCAGCTTC GAAGTGTCTG CCATTGCTTG 
TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAA AAAGGAAAAC 
AAAAAATCAG TGGTTAGCCA ACGTTTCCCT CAGAACAGCA TCGGTGCGGT AGGAAGTGCC 
ATGTTCCTCA GATTTATCAA TCCTGCCATT GTCTCGCCAT ATGAAGCAGG GATTTTAGAT 
AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGC 
ATAGCCAATC ATGTCCTGTT CACAAAAGAA GAACATATGA GGCCTTTTAA TGATTTTGTG 
AAAAGCAACT TTGACTTGGC ACGAAGATTT TTCCTTGATA TAGCATCAGA TTGTCCCACA 
AGTGATGCTG TAAATCATAG TCTTTCCTTC ATTAGTGATG GCAATGTACT TGCTTTACAT 
CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGCAA CAGGGATCAT 
AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCATGC TTCTTGCATA CCTGGGTCCT 
CCAGAGCATA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCCAAG 
TTTGAGGAGT TTATGACCAG GCATCAGGTA CATGAGAAAG AAGAGTTCAA GGCTTTGAAA 
ACTTTAAGTA TCTTCTACCA AGCTGGCACT TCTAAAGCTG GGAATCCTAT ATTTTATTAT 
GTTGCACGAA GGTTCAAAAC TGGCCAAATC AATGGTGATT TGCTGATATA CCATGTCTTG 
CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT 
GGACCTAGCA ATCGCTTTAA AACAGATTTT CTCTCTAAGT GGTTTGTTGT TTTTCCTGGC 
TTTGCTTACG ACAATGTCTC TGCAGTTTAT ATTTATAACT GTAACTCCTG GGTCAGAGAG 
TACACCAAGT ACCATGAACG GCTACTGACT GGCCTCAAGG GCAGCAAACG GCTTATTTTC 
ATAGACTGTC CTGGGAAGCT GGCTGAGCAC ATAGAGCATG AACAACAGAA ACTCCCTGCT 
GCCACATTGG CTCTGGAGGA GGACCTTAAG GTGTTCCACA ATGCTCTGAA GCTAGCTCAC 
AAAGATACCA AAGTCTCTAT TAAGGTTGGT TCTACTGCTG TTCAAGTAAC CTCAGCAGAG 
AGAACAAAAG TTTTGGGGCA ATCTGTGTTT TTAAATGACA TCTACTATGC TTCTGAAATC 
GAAGAGATCT GCCTTGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAAGGCACA 
CCACTTACCT TCATGCACCA GGAGTGTGAA GCCATTGTCC AGTCTATTAT TCACATCAGG 
ACCCGCTGGG AGCTATCACA GCCTGACTCT ATCCCCCAAC ATACCAAGAT TCGACCAAAA 
GATGTTCCTG GGACACTTCT TAATATTGCA TTACTTAATT TAGGCAGTTC AGACCCTAGC 
CTACGGTCTG CTGCCTATAA TCTTCTGTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAAGGCCAGT TACTAGAGAC ATCAGGGTTA TGCATCCCTG CCAACAATAC CCTCTTTATT 
GTCTCAATCA GTAAGACACT GGCCGCCAAT GAGCCACATC TCACCTTAGA GTTTTTGGAA 
GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT 
ATGACCCCGT GGCTATCAAA TCTAGTTCGT TTTTGTAAGC ACAATGATGA TGCCAAACGA 
CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CCATAAACGA GAAGCAGATG 
TACCCTTCTA TTCAAGCAAA AATATGGGGA AGCCTTGGGC AGATCACAGA CCTGCTGGAC 
GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCTACAGGAG GCCTGGGGTC TATCAAAGCT 
GAGGTGATGG CAGATACGGC TGTGGCTTTG GCTTCTGGAA ATGTGAAATT GGTATCAAGT 
AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCTCC AACTCCAACT 
TTAGAACAAC ATCTTATGTG GGATGATATT GCTATTTTAG CCCGCTACAT GCTGATGCTG 
TCCTTCAACA ATTCCCTGGA TGTGGCAGCT CACCTTCCCT ATCTCTTCCA TGTTGTTACT 
TTCTTAGTAG CCACAGGTCC CTTGTCCCTT CGAGCTTCCA CACATGGACT GGTCATTAAT 
ATCATTCACT CTCTGTGTAC TTGTTCACAG CTTCATTTTA GTGAAGAGAC CAAGCAAGTT 
TTGAGGCTCA GTCTAACAGA ATTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC 
AAAGTGAAGT CAGCTGCTGT GATTGCCTTC CGTTCCAGCT ACCGGGACCG GTCTTTCTCC 
CCAGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCC TGGAGACTGT CACAGAGGCT 
TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CGTGCAAGTG GCTGGATCAG 
TGGACAGAGC TAGCACAAAG GTTTGCATTC CAGTACAACC CATCCCTGCA GCCAAGAGCT 
CTTGTGGTGT TTGGCTGCAT AAGCAAGCGT GTATCTCATG GACAGATAAA GCAGATCATC 
CGAATTCTTA GCAAGGCACT TGAAAGTTGC TTAAAAGGCC CTGATACTTA CAACAGTCAA 
GTTCTGATAG AAGCTACAGT GATAGCTCTA ACAAAATTAC AGCCACTTCT TAATAAGGAC 
TCACCACTGC ACAAAGCCCT CTTTTGGGTT GCCGTGGCTG TGCTGCAGCT GGATGAAGTC 
AACTTGTATT CAGCAGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT AGATAGTCTC 
CGGATATTCA ATGACAAGAG TCCAGAAGAA GTATTTATGG CAATCCGGAA TCCTCTGGAG 
TGGCACTGCA AGCAAATGGA TCACTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT 
GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA 
ACAGTCAGAA TTTTGCATAC ACTACTAACT CTTGTTAACA AACATAGAAA TTGTGACAAA 
TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG 
GTTCGAAGTC GCTGTAGCCT CAAGCATAGA AAGTCCCTTC TTCTAACTGA TATTTCGATG 
GAAAATGTTC CTATGGATAC ATACCCCATT CTTCATGGTG ATCCTAGCTA TAGGACATTA 
AAGGAGAACC AGCCATGGTC CTCTCCCAAG GGTTCAGAAG GATACCTTGC AGCTACCTAC 
CCATCTGTAG GCCAGACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA CATGGGACAG 
CCTTCTCAAG CCAACACAAA GAAATTACTT GGAACAAGAA AAAGTTTTGA TCACTTGATA 
TCAGACACAA AGGCTCCCAA AAGACAAGAA ATGGAATCAG GGATCACAAC ACCCCCCAAA 
ATGAGAAGAG TGGCAGAAAC TGATTATGAA ATGGAAACCC AAAGGATTTC CTCACAGCAG 
CACCCACATT TACGGAAAGT CTCTGTGTCT GAATCAAATG TTCTTTTGGA TGAAGAAGTA 
CTTACTGATC CAAAGATACA AGCACTGCTT CTTACTGTTC TGGCTACACT GGTAAAATAT 
ACTACAGATG AATTTGATCA ACGAATTCTT TATGAATACC TAGCAGAGGC CAGTGTTGTG 
TTTCCCAAAG TGTTTCCTGT TGTGCACAAT TTGTTGGACT CTAAGATCAA TACCCTCTTG 
TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCAATCCATG GAATTGTGCA GAGTGTGGTG 
TACCATGAGG AATCCCCTCC ACAGTACCAA ACATCTTACC TGCAAAGTTT TGGTTTCAAT 
GGCTTGTGGC GTTTTGCAGG ACCCTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG 
CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTACT TGCCTGGAAT TGATGAAGAA 
GCCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTACC CTCCTGCACT GCAGAGCCAG 
CTTAGTATCA CTGCCAACCT TAACCTCTCT AATTCCATGA CCTCACTTGC AACTTCCCAG 
CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CCACCACTGG CCACTGTAAC 
AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAGGTGCAGA AGCAAAGAAG CGCTGGCAGT 
TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021086798.1
CDS	405..8957
Translation	MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDDNNINKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLFLCGVGCDVPASGNTTQMSMDHDELLRTCTPGASLRKGKGNSSMDSTAGCSGTPPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSSMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSSSGLATYSPPMGPVSERKGSMISVMSSEGNVDTPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDSNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNVVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLESTESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMAMLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPILHGDPSYRTLKENQPWSSPKGSEGYLAATYPSVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Target ORF information:

RefSeq Version	XM_021231139.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus neurofibromin 1 (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021231139.1

ORF Insert Sequence:

ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAAAATACA CATACCAAAG TCAGCACCGA GCATAACAAG 
GAATGTCTGA TCAACATTTC CAAATACAAG TTTTCTTTGG TCATAAGTGG CCTCACTACC 
ATCTTGAAGA ATGTTAATAA TATGCGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT 
CTTTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC 
ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTAC TGCCAGAAAT CTGCCATTTT 
CTTCACACCT GTCGTGAAGG AAACCAACAT GCGGCTGAAC TTCGGAATTC TGCTTCTGGG 
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG 
TTACAGGAGT TGACTGTTTG TTCAGAAGAC AATGTTGATG TCCATGATAT AGAATTGTTA 
CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA 
TTTAAAGCCC TGAAGAAGGT CGCACAGTTA GCAGTTATTA ATAGCCTAGA AAAGGCTTTT 
TGGAACTGGG TAGAAAACTA TCCGGATGAA TTTACAAAGC TGTACCAAAT TCCACAGACC 
GATATGGCTG AGTGTGCAGA AAAGCTGTTT GACTTGGTGG ACGGTTTTGC TGAAAGCACC 
AAACGTAAAG CCGCAGTTTG GCCACTGCAA ATCATTCTCC TCATTTTGTG TCCAGAAATA 
ATCCAGGATA TATCCAAGGA TGTAGTTGAT GACAACAACA TAAATAAGAA GCTGTTTCTG 
GATAGTTTAC GAAAAGCACT TGCTGGCCAT GGAGGAAGCA GGCAACTGAC AGAGAGTGCT 
GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTATA TCAACTGGGA AGATAACTCT 
GTCATTTTCC TACTAGTTCA GTCCATGGTG GTTGATCTGA AGAACCTGCT TTTTAATCCA 
AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT 
GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAGCACT TTAAGATCTG CCTGGCGCAG 
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAACTCAC TCCACCGCAT CATCACCAAT 
AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT 
GCTGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA 
GTGCAGGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTTAC ATTTAAAGAA 
AAGGTAACAA GTCTTAAATT TAAAGAAAAG CCTACAGACC TGGAAACAAG GAGCTATAAG 
TATCTTCTCT TGTCCATGGT GAAACTAATT CACGCAGACC CAAAGCTTTT ACTTTGTAAT 
CCAAGAAAAC AGGGCCCTGA AACTCAAGGC AGTACGGCAG AATTAATCAC AGGGCTTGTC 
CAACTGGTCC CTCAGTCACA TATGCCAGAG ATTGCTCAGG AAGCGATGGA GGCCTTGCTG 
GTTCTTCATC AGTTAGATAG CATTGATTTA TGGAACCCTG ATGCTCCTGT AGAGACATTT 
TGGGAAATTA GTTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG 
CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG GAATAAATTT 
CTTCTTAAAA ATAAGCAGGC GGATAGAAGT TCCTGTCACT TTCTCTTCCT TTGTGGAGTA 
GGATGTGATG TTCCTGCTAG TGGAAATACC ACTCAGATGT CAATGGATCA TGATGAGTTA 
TTAAGGACCT GCACTCCTGG AGCATCTTTG CGGAAAGGAA AAGGAAATTC CTCCATGGAT 
AGCACAGCAG GGTGCAGTGG AACCCCACCA ATATGCCGAC AAGCCCAGAC CAAGCTAGAA 
GTGGCTTTGT ACATGTTTCT GTGGAGTCCT GACACTGAAG CTGTTCTGGT TGCTATGTCT 
TGTTTCCGCC ACCTGTGTGA AGAAGCAGAT ATCAGGTGTG GGGTAGATGA AGTGTCAGTA 
CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCTGTCAG CAGTATGATG 
TCAACAGGAA GAGCAGCCCT TCAGAAGAGA GTGATGGCCC TGCTGAGGCG CATTGAGCAC 
CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAATGGGA ACAGGCTACA 
AAACTAATCC TTAACTATCC GAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGCCTT 
CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGTGGATC CATAGACTTG 
TCTGACACAG ACTCGCTGCA GGAATGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT 
GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCTTAGCGA CCTACAGCCC ACCTATGGGC 
CCTGTCAGTG AGCGCAAAGG GTCTATGATT TCCGTAATGT CTTCTGAAGG GAATGTTGAC 
ACACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCTTTAA TGGTGTGTAA CCACGAGAAA 
GTGGGGCTTC AGATACGAAC CAATGTTAAG GACCTGGTGG GCCTGGAGTT GAGTCCTGCT 
CTGTATCCAA TGTTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA 
GGACAGGTAT TATTGACTGA TAGCAATACT CAGTTTGTAG AGCAAACTAT AGCCATAATG 
AAGAACCTGC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCTAGCATT 
GAAACAATGA TGTTAAATCT GGTTAGATAC GTTCGTGTAC TTGGGAATGT GGTCCATGCA 
ATTCAAATAA AAACAAAACT GTGCCAGTTA GTTGAAGTAA TGATGGCAAG AAGAGATGAT 
CTCTCATTTT GTCAGGAGAT GAAATTTAGG AATAAAATGG TAGAATACCT AACAGACTGG 
GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AGTGTCTTAC AAGAGATTTG 
GACCAAGCAA GCATGGAAGC AGTAGTTTCA CTCCTCGCTG GTCTTCCACT ACAGCCTGAA 
GAAGGAGATG GGGTGGAACT AATGGAGGCC AAATCACAGT TATTTCTTAA GTACTTCACA 
CTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT 
GGCAGAAAAC GTGGCATGTC TCGGAGGCTG GCATCCTTGA GGCACTGTAC AGTGCTTGCA 
ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT 
TATCACAAGG ATCTCCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA 
CAAGGAACAG AATTTGACAC ACTTGCTGAA ACGGTGTTGG CTGATCGGTT TGAGAGACTC 
GTGGAACTGG TCACAATGAT GGGAGACCAA GGAGAGCTCC CTATAGCTAT GGCTCTGGCC 
AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGTG TTCTGGTTAC TCTGTTTGAT 
TCTCGGCACT TACTCTACCA GCTGCTTTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA 
GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC 
TTTAAGGTTT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ATCCTTTATT ACGTATTGTG 
ATCACATCCT CAGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAATCT 
ACTGAGAGCC TTGAGGAAAA CCAGAGGAAC CTCCTTCAAA TGACAGAGAA GTTCTTCCAT 
GCCATCATCA GTTCCTCCTC AGAATTCCCC TCCCAGCTTC GAAGTGTCTG CCATTGCTTG 
TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAA AAAGGAAAAC 
AAAAAATCAG TGGTTAGCCA ACGTTTCCCT CAGAACAGCA TCGGTGCGGT AGGAAGTGCC 
ATGTTCCTCA GATTTATCAA TCCTGCCATT GTCTCGCCAT ATGAAGCAGG GATTTTAGAT 
AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGC 
ATAGCCAATC ATGTCCTGTT CACAAAAGAA GAACATATGA GGCCTTTTAA TGATTTTGTG 
AAAAGCAACT TTGACTTGGC ACGAAGATTT TTCCTTGATA TAGCATCAGA TTGTCCCACA 
AGTGATGCTG TAAATCATAG TCTTTCCTTC ATTAGTGATG GCAATGTACT TGCTTTACAT 
CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGCAA CAGGGATCAT 
AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCATGC TTCTTGCATA CCTGGGTCCT 
CCAGAGCATA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCCAAG 
TTTGAGGAGT TTATGACCAG GCATCAGGTA CATGAGAAAG AAGAGTTCAA GGCTTTGAAA 
ACTTTAAGTA TCTTCTACCA AGCTGGCACT TCTAAAGCTG GGAATCCTAT ATTTTATTAT 
GTTGCACGAA GGTTCAAAAC TGGCCAAATC AATGGTGATT TGCTGATATA CCATGTCTTG 
CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT 
GGACCTAGCA ATCGCTTTAA AACAGATTTT CTCTCTAAGT GGTTTGTTGT TTTTCCTGGC 
TTTGCTTACG ACAATGTCTC TGCAGTTTAT ATTTATAACT GTAACTCCTG GGTCAGAGAG 
TACACCAAGT ACCATGAACG GCTACTGACT GGCCTCAAGG GCAGCAAACG GCTTATTTTC 
ATAGACTGTC CTGGGAAGCT GGCTGAGCAC ATAGAGCATG AACAACAGAA ACTCCCTGCT 
GCCACATTGG CTCTGGAGGA GGACCTTAAG GTGTTCCACA ATGCTCTGAA GCTAGCTCAC 
AAAGATACCA AAGTCTCTAT TAAGGTTGGT TCTACTGCTG TTCAAGTAAC CTCAGCAGAG 
AGAACAAAAG TTTTGGGGCA ATCTGTGTTT TTAAATGACA TCTACTATGC TTCTGAAATC 
GAAGAGATCT GCCTTGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAAGGCACA 
CCACTTACCT TCATGCACCA GGAGTGTGAA GCCATTGTCC AGTCTATTAT TCACATCAGG 
ACCCGCTGGG AGCTATCACA GCCTGACTCT ATCCCCCAAC ATACCAAGAT TCGACCAAAA 
GATGTTCCTG GGACACTTCT TAATATTGCA TTACTTAATT TAGGCAGTTC AGACCCTAGC 
CTACGGTCTG CTGCCTATAA TCTTCTGTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAAGGCCAGT TACTAGAGAC ATCAGGGTTA TGCATCCCTG CCAACAATAC CCTCTTTATT 
GTCTCAATCA GTAAGACACT GGCCGCCAAT GAGCCACATC TCACCTTAGA GTTTTTGGAA 
GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT 
ATGACCCCGT GGCTATCAAA TCTAGTTCGT TTTTGTAAGC ACAATGATGA TGCCAAACGA 
CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CCATAAACGA GAAGCAGATG 
TACCCTTCTA TTCAAGCAAA AATATGGGGA AGCCTTGGGC AGATCACAGA CCTGCTGGAC 
GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCTACAGGAG GCCTGGGGTC TATCAAAGCT 
GAGGTGATGG CAGATACGGC TGTGGCTTTG GCTTCTGGAA ATGTGAAATT GGTATCAAGT 
AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCTCC AACTCCAACT 
TTAGAACAAC ATCTTATGTG GGATGATATT GCTATTTTAG CCCGCTACAT GCTGATGCTG 
TCCTTCAACA ATTCCCTGGA TGTGGCAGCT CACCTTCCCT ATCTCTTCCA TGTTGTTACT 
TTCTTAGTAG CCACAGGTCC CTTGTCCCTT CGAGCTTCCA CACATGGACT GGTCATTAAT 
ATCATTCACT CTCTGTGTAC TTGTTCACAG CTTCATTTTA GTGAAGAGAC CAAGCAAGTT 
TTGAGGCTCA GTCTAACAGA ATTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC 
AAAGTGAAGT CAGCTGCTGT GATTGCCTTC CGTTCCAGCT ACCGGGACCG GTCTTTCTCC 
CCAGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCC TGGAGACTGT CACAGAGGCT 
TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CGTGCAAGTG GCTGGATCAG 
TGGACAGAGC TAGCACAAAG GTTTGCATTC CAGTACAACC CATCCCTGCA GCCAAGAGCT 
CTTGTGGTGT TTGGCTGCAT AAGCAAGCGT GTATCTCATG GACAGATAAA GCAGATCATC 
CGAATTCTTA GCAAGGCACT TGAAAGTTGC TTAAAAGGCC CTGATACTTA CAACAGTCAA 
GTTCTGATAG AAGCTACAGT GATAGCTCTA ACAAAATTAC AGCCACTTCT TAATAAGGAC 
TCACCACTGC ACAAAGCCCT CTTTTGGGTT GCCGTGGCTG TGCTGCAGCT GGATGAAGTC 
AACTTGTATT CAGCAGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT AGATAGTCTC 
CGGATATTCA ATGACAAGAG TCCAGAAGAA GTATTTATGG CAATCCGGAA TCCTCTGGAG 
TGGCACTGCA AGCAAATGGA TCACTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT 
GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA 
ACAGTCAGAA TTTTGCATAC ACTACTAACT CTTGTTAACA AACATAGAAA TTGTGACAAA 
TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG 
GTTCGAAGTC GCTGTAGCCT CAAGCATAGA AAGTCCCTTC TTCTAACTGA TATTTCGATG 
GAAAATGTTC CTATGGATAC ATACCCCATT CTTCATGGTG ATCCTAGCTA TAGGACATTA 
AAGGAGAACC AGCCATGGTC CTCTCCCAAG GGTTCAGAAG GATACCTTGC AGCTACCTAC 
CCATCTGTAG GCCAGACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA CATGGGACAG 
CCTTCTCAAG CCAACACAAA GAAATTACTT GGAACAAGAA AAAGTTTTGA TCACTTGATA 
TCAGACACAA AGGCTCCCAA AAGACAAGAA ATGGAATCAG GGATCACAAC ACCCCCCAAA 
ATGAGAAGAG TGGCAGAAAC TGATTATGAA ATGGAAACCC AAAGGATTTC CTCACAGCAG 
CACCCACATT TACGGAAAGT CTCTGTGTCT GAATCAAATG TTCTTTTGGA TGAAGAAGTA 
CTTACTGATC CAAAGATACA AGCACTGCTT CTTACTGTTC TGGCTACACT GGTAAAATAT 
ACTACAGATG AATTTGATCA ACGAATTCTT TATGAATACC TAGCAGAGGC CAGTGTTGTG 
TTTCCCAAAG TGTTTCCTGT TGTGCACAAT TTGTTGGACT CTAAGATCAA TACCCTCTTG 
TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCAATCCATG GAATTGTGCA GAGTGTGGTG 
TACCATGAGG AATCCCCTCC ACAGTACCAA ACATCTTACC TGCAAAGTTT TGGTTTCAAT 
GGCTTGTGGC GTTTTGCAGG ACCCTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG 
CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTACT TGCCTGGAAT TGATGAAGAA 
GCCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTACC CTCCTGCACT GCAGAGCCAG 
CTTAGTATCA CTGCCAACCT TAACCTCTCT AATTCCATGA CCTCACTTGC AACTTCCCAG 
CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CCACCACTGG CCACTGTAAC 
AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAGGTGCAGA AGCAAAGAAG CGCTGGCAGT 
TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters.
Scientific reports740472(2017 01)
McCann KE,Sinkiewicz DM,Norvelle A,Huhman KL

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Nf1 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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