Afdn cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afdn
Entrez Gene ID	101823227
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013122737.1	XP_012978191.1	afadin
XM_021234665.1	XP_021090324.1	afadin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters.
McCann KE, Sinkiewicz DM, Norvelle A, Huhman KL
Scientific reports740472(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Afdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe137159	XM_013122737.1 Latest version!	Mesocricetus auratus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe253779	XM_021234665.1 Latest version!	Mesocricetus auratus afadin, adherens junction formation factor (Afdn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afdn ( XM_013122737.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012978191.1 Mesocricetus auratus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), mRNA.

Price & Availability↑

CloneID	OMe137159
Clone ID Related Accession (Same CDS sequence)	XM_013122737.1
Accession Version	XM_013122737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	afadin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGGGG GCCACCCCTG CTGCAGCCCG CTGCAGCCGC GCCTGGTTGA GAATTCTCGA 
CTGGCTGCTG AAGTTTACAA AGATATGCCT GAAACCAGCT TTACGCGAAC AATTTCTAAC 
CCTGAGGTGG TTATGAAACG GCGGCGGCAG CAAAAGCTTG AAAAGAGAAT GCAAGAGTTT 
CGGAGCTCAG ATGGGCGGCC TGACTCAGGT GGAACATTGA GAATATATGC AGACAGTTTA 
AAACCAAATA TTCCATACAA GACAATCCTG CTGTCTACTA CAGACTCTGC AGACTTTGCT 
GTGGCGGAAT CTCTGGAGAA GTATGGTCTA GAAAAAGAGA GTCCCAAGGA CTACTGCATT 
GCCCGGGTTA TGCTTCCTCC TGGAGCCCAG CATTCTGATG AAAGAGGTGC TAAGGAAGTT 
ATTCTTGATG ATGATGAGTG TCCTTTGCAG ATCTTCAGGG AATGGCCAAG TGACAAAGGG 
ATTTTAGTCT TTCAATTGAA GAGGAGGCCA CCGGACTATA TCCCAAAGAA AATGAAGAAG 
CATGTTGAAG GGAAGCCGCT GAAGGGGAAG GATAGGGTCG ACGGGTCTGG CTATGGTTCT 
ACTCTTCCTC CTGAGAAACT GCCCTACTTA GTAGAGTTAA GTCCAGGGAG AAGGAGTCAC 
TTTGCCTACT GCAGCTATCA CACTTACGAA GATGGCTCTG ACTCCAGAGA CAAGCCAAAG 
TTATACCGCC TTCAATTAAG TGTGACTGAA GTCGGGACAG AGAAGTTCGA TGACAATTCT 
ATCCAGTTGT TTGGCCCAGG AATTCAGCCC CATCACTGTG ACCTCACTAA CATGGATGGA 
GTGGTCACTG TGACACCCAG AAGTGTGGAT GCAGAGACGT ACGTGGATGG CCAGCGCATC 
TCGGAGACGA CCATGCTGCA GAGCGGCATG ACAGTGCAGT TCGGCTCCTC ACATGTGTTC 
AAGTTTGTGG ACCCCATTCA GGACCACGCT CTTTCGAAGA GGTCTGTGGA TGGAGGCCTG 
ATGGTCAAGG GCCCAAGACA TAAACCTGGA GCCATTCAGG AGACAACCTT TGATTTGGGA 
GGAGATATTC ACAGTGGGAC AGCATTGCCA GTAAGCAGGA GTACTACTAG ACTGGACAGT 
GACAGAGTGT CCTCTGCCTC CAGCACAGCT GAGCGAGGGA TGGTGAAGCC AATGATCCGC 
CTGGACCAGG AGCAGGATTA CCGACGAAGA GAAAGCAGAA CTCAGGATGG GGCTGGGCCT 
GAACTGACAC TGCCTGCCAG CGTTGAATTC AGGGAAAGCT CTGAAGATTC TTTTTTATCT 
GCCATTATAA ACTATACCAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTG 
TTATACATGG CGTGCCGGTA TGTATTGTCC AGCCAGCACA GGCCCGACAT CAGTCCGACA 
GAGCGCACAC ATAAGGCCAT TGCTGTCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTC 
ATTCAGGAAG TAGACCAGGT TGATCAGAAA CAAAAGAACA TTGCAGGGGC GCTTGCGTTC 
TGGATGGCGA ATGCATCTGA ACTTCTCAAC TTCATCAAGC AGGACCGAGA CCTTAGTCGG 
ATCACACTAG ATGCCCAGGA CGTCTTAGCA CACTTGGTTC AGATGGCATT TAAGTACTTG 
GTTCACTGTC TTCAGTCAGA ACTTAATAAC TACATGCCAG CCTTTCTGGA TGATCCAGAG 
GAGAACAGTC TGCAAAGACC AAAAATAGAT GACGTGCTGC ACACACTCAC AGGAGCCATG 
TCCTTGCTGA GGCGCTGCAG AGTCAATGCT GCCCTGACCA TCCAGCTGTT CTCTCAGCTT 
TTCCATTTTA TCAACATGTG GCTGTTCAAC AGACTGGTGA CTGACCCAGA CTCGGGCCTG 
TGCTCCCACT ACTGGGGAGC GATTATCCGC CAGCAGCTAG GGCACATTGA AGCCTGGGCG 
GAGAAGCAGG GCCTGGAGCT GGCAGCTGAC TGTCACTTGA GCAGGATTGT GCAGGCTACT 
ACTTTGCTTA CCATGGATAA GTATGTACCT GATGACATTA CAAATATAAA CAGTACGTGC 
TTTAAGTTAA ATTCTCTGCA ACTTCAAGCC TTGTTACAGA ACTACCACTG TGCACCCGAT 
GAGCCTTTTA TTCCTACGGA TCTCATAGAA AATGTTGTGG CTGTTGCTGA GAACACAGCT 
GATGAGCTTG CCCGCAGTGA CGGCAGAGAT GTGCAGTTGG AGGAGGACCC AGACCTACAG 
CTACCCTTTC TCTTGCCAGA AGATGGCTAT TCCTGTGATG TCGTCAGAAA CATTCCAAAT 
GGTTTACAAG AGTTTTTAGA CCCTCTGTGC CAGAGAGGAT TTTGCAGATT GGTTCCTCAT 
ACACGTTCAC CAGGTACTTG GACAATATAT TTTGAAGGTG CAGATTATGA AAGTCACCTT 
ATGCGTGAGA ACACAGAACT GGCCCAGCCT CTGAGGAAGG AACCTGAGAT AATCACGGTG 
ACCCTAAAAA AACAGAATGG AATGGGCCTC AGCATTGTTG CAGCAAAGGG TGCTGGCCAG 
GATAAACTGG GAATCTACGT GAAGTCTGTT GTGAAAGGAG GAGCTGCAGA TGTGGATGGA 
CGTCTAGCTG CTGGGGACCA GCTCCTCAGT GTGGATGGAC GAAGTCTGGT TGGACTTTCT 
CAGGAAAGGG CAGCAGAACT CATGACAAGA ACCAGTTCAG TGGTCACACT TGAAGTTGCA 
AAGCAAGGCG CCATCTATCA CGGCCTGGCC ACCCTCCTTA ACCAGCCATC CCCCATGATG 
CAGAGAATTT CAGATCGTCG CGGCTCAGGT AAACCCAGAC CAAAGAGTGA AGGTTTTGAG 
CTCTATAATA ATTCAGGTCA AAATGGCTCC CCAGAGAGTC CTCAGATGCC TTGGACAGAG 
TACAGTGAGC CAAAGAAACT TCCTGGTGAC GACAGGCTGA TGAAAAACAG AGCAGATCAC 
CGTTCCAGCC CTAATGTGGC AAATCAGCCT CCTAGTCCTG GAGGGAAGAG TCCATATGCC 
TCTGGAACAG CGGCTAAGAT AACATCTGTC TCCACTGGAA ACCTGTGCAC TGAGGAGCAG 
ACCCCTCCAC CTAGACCTGA AGCCTATCCC ATCCCTACTC AGACATACAC CAGAGAGTAT 
TTTACCTTTC CAGCTTCAAA ATCCCAGGAC CGGATGGCTC CTCCTCAGAA CCAGTGGCCA 
AATTATGAGG AAAAGCCACA TATGCACACA GAAAGTAATC ATTCCAGTAT TGCAATCCAG 
CGTGTTACCC GTTCCCAGGA AGAGCTTCGA GAAGAGAAGG GTTACCAGCT TGAGCGGCAT 
CGAGCAGAGG CTGGCATGGA TCGCAAGTGT GACAGTGACA TGTGGATCAA TCAGAGCTCC 
TCAGTGGATT CCAGCACATC CAGCCAAGAG CACCTGAACC ACTCCTCCAA GTCAGTCACC 
CCTGCTTCCA CTCTGACCAA AAGCGGTCCC GGACGTTGGA AAACCCCCGC AGCAGTACTG 
CCTACCCCTG TGGCTGTCTC CCAGCCCATT CGAACAGACC TCCCTCCCCC ACCCCCTCCG 
CCTCCTGCCC ACTACACCAG TGATTTTGAT GGCATTCCAG TGGATCTGCC CCTCCCACCT 
CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT GCTCAGGTGG CTGCTGCAGA GAGGAAAAAG 
AGAGAAGAGC ATCAGCGGTG GTATGAGAAA GAGAAGGCCC GCCTCGAGGA GGAGCGCGAG 
AGGAAGCGCA GGGAACAGGA GCGGAAGTTG GGGCAGATGC GCACGCAGTC CCTGAACCCT 
GCTTCCTTCT CTCCTTTGGC CACACAGGCC AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG 
CCCCAAGAAA CAGTCATCCG GGAGCTGCAG CCCCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGGGACCTGC AGTACGTCAC CATCAGTAAA GAAGAGCTGT CCTCTGGGGA CAGTCTGTCT 
CCAGACCCCT GGAAACGAGA TGCCAGGGAG AAGCTGGAGA AGCAGCAGCA AATGCACATC 
GTCGACATGC TGAGCAAGGA GATCCAGGAA CTGCAGAACA AGGTGGACCG CACCGCAGAG 
GAGAGTGACC GCCTGCGGAA GCTTATGCTG GAATGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA AGGATGAGGA TGACGAGGAA GAGGAAGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GTCTGGAGGC TGAGCGGAGA GCCAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA TGAGGAGCGC AGGCGCCAGC AGCAGTTGGA AGAGATGCGC 
AAACGAGAAG CAGAAGATCG TGTAAGACAA GAGGAAGATC GACGCCATCA GGAGGAGGAG 
CGAGTAAAGA GAGATGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTA 
GAAGCTGAGA GGCGCAGACA GCACGACGAG GCAGCACGAA GGTTGCTGGA GCCCGAAGAG 
CCTGGGCTGA GCCGACCTCC ACTTCCACGG GACTATGAGC CCCCATCCCC GTCCCCAGCA 
CCCAGCGCCC CTCCTCCCCC ACCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCAGACT CGCTGTTCAC TGCCAAGTTT GTTGCATATG ATGATGATGA GGAGGAGGAC 
TGCGGCCCAG CAGGTCAGGA TAAGTACCCC AGCACAAGGA GGTCTCATGA GCACCTTCCT 
CTTGCTGCCC CTAAGCCGCA GCAGCCCCCC TGCCAGCCAC GCCCAGCCTC GGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTTG GCCAAAGCCA ACAGTACTGC TAACAAAGCA 
GGCCAGCCCC CACCCCCTCC AGGCAAACCG AGCTTCTGCC GTCCTGGCCC CTGGAAAGGA 
CCACACTCTT ATACGGGATC TGCTGGAACA GCTTCAGCAG TCCATGATGT TCCTCGGGAC 
CCAAGAGAGA AGCACACTAG AAGCCAAGAT GCAGACTTTC CAGGCAGTTC TGGAGCCCCT 
GAAAACCTGA CATTTAAAGA GCGTCAGCGC CTCTTTTCAC AAGGTCAAGA TGTGTCTGAC 
AAAGTAAAAG CTTCTCGTAA ATTAACAGAA CTTGAGAATG AACTGAACAC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012978191.1
CDS	1..5217
Translation	MAGGHPCCSPLQPRLVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDSADFAVAESLEKYGLEKESPKDYCIARVMLPPGAQHSDERGAKEVILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRVDGSGYGSTLPPEKLPYLVELSPGRRSHFAYCSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSVDAETYVDGQRISETTMLQSGMTVQFGSSHVFKFVDPIQDHALSKRSVDGGLMVKGPRHKPGAIQETTFDLGGDIHSGTALPVSRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDGAGPELTLPASVEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDITNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSGQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYASGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTESNHSSIAIQRVTRSQEELREEKGYQLERHRAEAGMDRKCDSDMWINQSSSVDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGIPVDLPLPPPPANQAGPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYVTISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIQELQNKVDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRVRQEEDRRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEEPGLSRPPLPRDYEPPSPSPAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDEEEDCGPAGQDKYPSTRRSHEHLPLAAPKPQQPPCQPRPASDGIFLSNSFQPPLAKANSTANKAGQPPPPPGKPSFCRPGPWKGPHSYTGSAGTASAVHDVPRDPREKHTRSQDADFPGSSGAPENLTFKERQRLFSQGQDVSDKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_013122737.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013122737.1

ORF Insert Sequence:

ATGGCGGGGG GCCACCCCTG CTGCAGCCCG CTGCAGCCGC GCCTGGTTGA GAATTCTCGA 
CTGGCTGCTG AAGTTTACAA AGATATGCCT GAAACCAGCT TTACGCGAAC AATTTCTAAC 
CCTGAGGTGG TTATGAAACG GCGGCGGCAG CAAAAGCTTG AAAAGAGAAT GCAAGAGTTT 
CGGAGCTCAG ATGGGCGGCC TGACTCAGGT GGAACATTGA GAATATATGC AGACAGTTTA 
AAACCAAATA TTCCATACAA GACAATCCTG CTGTCTACTA CAGACTCTGC AGACTTTGCT 
GTGGCGGAAT CTCTGGAGAA GTATGGTCTA GAAAAAGAGA GTCCCAAGGA CTACTGCATT 
GCCCGGGTTA TGCTTCCTCC TGGAGCCCAG CATTCTGATG AAAGAGGTGC TAAGGAAGTT 
ATTCTTGATG ATGATGAGTG TCCTTTGCAG ATCTTCAGGG AATGGCCAAG TGACAAAGGG 
ATTTTAGTCT TTCAATTGAA GAGGAGGCCA CCGGACTATA TCCCAAAGAA AATGAAGAAG 
CATGTTGAAG GGAAGCCGCT GAAGGGGAAG GATAGGGTCG ACGGGTCTGG CTATGGTTCT 
ACTCTTCCTC CTGAGAAACT GCCCTACTTA GTAGAGTTAA GTCCAGGGAG AAGGAGTCAC 
TTTGCCTACT GCAGCTATCA CACTTACGAA GATGGCTCTG ACTCCAGAGA CAAGCCAAAG 
TTATACCGCC TTCAATTAAG TGTGACTGAA GTCGGGACAG AGAAGTTCGA TGACAATTCT 
ATCCAGTTGT TTGGCCCAGG AATTCAGCCC CATCACTGTG ACCTCACTAA CATGGATGGA 
GTGGTCACTG TGACACCCAG AAGTGTGGAT GCAGAGACGT ACGTGGATGG CCAGCGCATC 
TCGGAGACGA CCATGCTGCA GAGCGGCATG ACAGTGCAGT TCGGCTCCTC ACATGTGTTC 
AAGTTTGTGG ACCCCATTCA GGACCACGCT CTTTCGAAGA GGTCTGTGGA TGGAGGCCTG 
ATGGTCAAGG GCCCAAGACA TAAACCTGGA GCCATTCAGG AGACAACCTT TGATTTGGGA 
GGAGATATTC ACAGTGGGAC AGCATTGCCA GTAAGCAGGA GTACTACTAG ACTGGACAGT 
GACAGAGTGT CCTCTGCCTC CAGCACAGCT GAGCGAGGGA TGGTGAAGCC AATGATCCGC 
CTGGACCAGG AGCAGGATTA CCGACGAAGA GAAAGCAGAA CTCAGGATGG GGCTGGGCCT 
GAACTGACAC TGCCTGCCAG CGTTGAATTC AGGGAAAGCT CTGAAGATTC TTTTTTATCT 
GCCATTATAA ACTATACCAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTG 
TTATACATGG CGTGCCGGTA TGTATTGTCC AGCCAGCACA GGCCCGACAT CAGTCCGACA 
GAGCGCACAC ATAAGGCCAT TGCTGTCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTC 
ATTCAGGAAG TAGACCAGGT TGATCAGAAA CAAAAGAACA TTGCAGGGGC GCTTGCGTTC 
TGGATGGCGA ATGCATCTGA ACTTCTCAAC TTCATCAAGC AGGACCGAGA CCTTAGTCGG 
ATCACACTAG ATGCCCAGGA CGTCTTAGCA CACTTGGTTC AGATGGCATT TAAGTACTTG 
GTTCACTGTC TTCAGTCAGA ACTTAATAAC TACATGCCAG CCTTTCTGGA TGATCCAGAG 
GAGAACAGTC TGCAAAGACC AAAAATAGAT GACGTGCTGC ACACACTCAC AGGAGCCATG 
TCCTTGCTGA GGCGCTGCAG AGTCAATGCT GCCCTGACCA TCCAGCTGTT CTCTCAGCTT 
TTCCATTTTA TCAACATGTG GCTGTTCAAC AGACTGGTGA CTGACCCAGA CTCGGGCCTG 
TGCTCCCACT ACTGGGGAGC GATTATCCGC CAGCAGCTAG GGCACATTGA AGCCTGGGCG 
GAGAAGCAGG GCCTGGAGCT GGCAGCTGAC TGTCACTTGA GCAGGATTGT GCAGGCTACT 
ACTTTGCTTA CCATGGATAA GTATGTACCT GATGACATTA CAAATATAAA CAGTACGTGC 
TTTAAGTTAA ATTCTCTGCA ACTTCAAGCC TTGTTACAGA ACTACCACTG TGCACCCGAT 
GAGCCTTTTA TTCCTACGGA TCTCATAGAA AATGTTGTGG CTGTTGCTGA GAACACAGCT 
GATGAGCTTG CCCGCAGTGA CGGCAGAGAT GTGCAGTTGG AGGAGGACCC AGACCTACAG 
CTACCCTTTC TCTTGCCAGA AGATGGCTAT TCCTGTGATG TCGTCAGAAA CATTCCAAAT 
GGTTTACAAG AGTTTTTAGA CCCTCTGTGC CAGAGAGGAT TTTGCAGATT GGTTCCTCAT 
ACACGTTCAC CAGGTACTTG GACAATATAT TTTGAAGGTG CAGATTATGA AAGTCACCTT 
ATGCGTGAGA ACACAGAACT GGCCCAGCCT CTGAGGAAGG AACCTGAGAT AATCACGGTG 
ACCCTAAAAA AACAGAATGG AATGGGCCTC AGCATTGTTG CAGCAAAGGG TGCTGGCCAG 
GATAAACTGG GAATCTACGT GAAGTCTGTT GTGAAAGGAG GAGCTGCAGA TGTGGATGGA 
CGTCTAGCTG CTGGGGACCA GCTCCTCAGT GTGGATGGAC GAAGTCTGGT TGGACTTTCT 
CAGGAAAGGG CAGCAGAACT CATGACAAGA ACCAGTTCAG TGGTCACACT TGAAGTTGCA 
AAGCAAGGCG CCATCTATCA CGGCCTGGCC ACCCTCCTTA ACCAGCCATC CCCCATGATG 
CAGAGAATTT CAGATCGTCG CGGCTCAGGT AAACCCAGAC CAAAGAGTGA AGGTTTTGAG 
CTCTATAATA ATTCAGGTCA AAATGGCTCC CCAGAGAGTC CTCAGATGCC TTGGACAGAG 
TACAGTGAGC CAAAGAAACT TCCTGGTGAC GACAGGCTGA TGAAAAACAG AGCAGATCAC 
CGTTCCAGCC CTAATGTGGC AAATCAGCCT CCTAGTCCTG GAGGGAAGAG TCCATATGCC 
TCTGGAACAG CGGCTAAGAT AACATCTGTC TCCACTGGAA ACCTGTGCAC TGAGGAGCAG 
ACCCCTCCAC CTAGACCTGA AGCCTATCCC ATCCCTACTC AGACATACAC CAGAGAGTAT 
TTTACCTTTC CAGCTTCAAA ATCCCAGGAC CGGATGGCTC CTCCTCAGAA CCAGTGGCCA 
AATTATGAGG AAAAGCCACA TATGCACACA GAAAGTAATC ATTCCAGTAT TGCAATCCAG 
CGTGTTACCC GTTCCCAGGA AGAGCTTCGA GAAGAGAAGG GTTACCAGCT TGAGCGGCAT 
CGAGCAGAGG CTGGCATGGA TCGCAAGTGT GACAGTGACA TGTGGATCAA TCAGAGCTCC 
TCAGTGGATT CCAGCACATC CAGCCAAGAG CACCTGAACC ACTCCTCCAA GTCAGTCACC 
CCTGCTTCCA CTCTGACCAA AAGCGGTCCC GGACGTTGGA AAACCCCCGC AGCAGTACTG 
CCTACCCCTG TGGCTGTCTC CCAGCCCATT CGAACAGACC TCCCTCCCCC ACCCCCTCCG 
CCTCCTGCCC ACTACACCAG TGATTTTGAT GGCATTCCAG TGGATCTGCC CCTCCCACCT 
CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT GCTCAGGTGG CTGCTGCAGA GAGGAAAAAG 
AGAGAAGAGC ATCAGCGGTG GTATGAGAAA GAGAAGGCCC GCCTCGAGGA GGAGCGCGAG 
AGGAAGCGCA GGGAACAGGA GCGGAAGTTG GGGCAGATGC GCACGCAGTC CCTGAACCCT 
GCTTCCTTCT CTCCTTTGGC CACACAGGCC AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG 
CCCCAAGAAA CAGTCATCCG GGAGCTGCAG CCCCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGGGACCTGC AGTACGTCAC CATCAGTAAA GAAGAGCTGT CCTCTGGGGA CAGTCTGTCT 
CCAGACCCCT GGAAACGAGA TGCCAGGGAG AAGCTGGAGA AGCAGCAGCA AATGCACATC 
GTCGACATGC TGAGCAAGGA GATCCAGGAA CTGCAGAACA AGGTGGACCG CACCGCAGAG 
GAGAGTGACC GCCTGCGGAA GCTTATGCTG GAATGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCAGA AGGATGAGGA TGACGAGGAA GAGGAAGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GTCTGGAGGC TGAGCGGAGA GCCAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA TGAGGAGCGC AGGCGCCAGC AGCAGTTGGA AGAGATGCGC 
AAACGAGAAG CAGAAGATCG TGTAAGACAA GAGGAAGATC GACGCCATCA GGAGGAGGAG 
CGAGTAAAGA GAGATGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTA 
GAAGCTGAGA GGCGCAGACA GCACGACGAG GCAGCACGAA GGTTGCTGGA GCCCGAAGAG 
CCTGGGCTGA GCCGACCTCC ACTTCCACGG GACTATGAGC CCCCATCCCC GTCCCCAGCA 
CCCAGCGCCC CTCCTCCCCC ACCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCAGACT CGCTGTTCAC TGCCAAGTTT GTTGCATATG ATGATGATGA GGAGGAGGAC 
TGCGGCCCAG CAGGTCAGGA TAAGTACCCC AGCACAAGGA GGTCTCATGA GCACCTTCCT 
CTTGCTGCCC CTAAGCCGCA GCAGCCCCCC TGCCAGCCAC GCCCAGCCTC GGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTTG GCCAAAGCCA ACAGTACTGC TAACAAAGCA 
GGCCAGCCCC CACCCCCTCC AGGCAAACCG AGCTTCTGCC GTCCTGGCCC CTGGAAAGGA 
CCACACTCTT ATACGGGATC TGCTGGAACA GCTTCAGCAG TCCATGATGT TCCTCGGGAC 
CCAAGAGAGA AGCACACTAG AAGCCAAGAT GCAGACTTTC CAGGCAGTTC TGGAGCCCCT 
GAAAACCTGA CATTTAAAGA GCGTCAGCGC CTCTTTTCAC AAGGTCAAGA TGTGTCTGAC 
AAAGTAAAAG CTTCTCGTAA ATTAACAGAA CTTGAGAATG AACTGAACAC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afdn ( XM_021234665.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021090324.1 Mesocricetus auratus afadin, adherens junction formation factor (Afdn), mRNA.

Price & Availability↑

CloneID	OMe253779
Clone ID Related Accession (Same CDS sequence)	XM_021234665.1
Accession Version	XM_021234665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	afadin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801746.1) and transcript sequences (GEMX01006961.1 and GEMX01006964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 612 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATCT GGAGTTTCAT 
GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAGTGCATT 
CGGGTATCTA GCACGGCAAC TACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC 
CCTGATATGC GCATGCTGTC CTCTCCCAAG TATTCTCTCT ATGAAGTGCA TGTCAGTGGA 
GAAGAAAGAA GATTAGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA CTGGAACAAA 
GATGATCGGG AAGGTAGATT CGTCCTTAAG AATGAGAATG ACGCCATTCC AGCCAAGAAG 
GCTCAGAGTA ATGGACCCGA GAAGCAGGAC AAGGAAGGCG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGGG AAAAAGAGGC ATTGAGACAA 
GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAATTC TCGACTGGCT 
GCTGAAGTTT ACAAAGATAT GCCTGAAACC AGCTTTACGC GAACAATTTC TAACCCTGAG 
GTGGTTATGA AACGGCGGCG GCAGCAAAAG CTTGAAAAGA GAATGCAAGA GTTTCGGAGC 
TCAGATGGGC GGCCTGACTC AGGTGGAACA TTGAGAATAT ATGCAGACAG TTTAAAACCA 
AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACAGACT CTGCAGACTT TGCTGTGGCG 
GAATCTCTGG AGAAGTATGG TCTAGAAAAA GAGAGTCCCA AGGACTACTG CATTGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAGAG GTGCTAAGGA AGTTATTCTT 
GATGATGATG AGTGTCCTTT GCAGATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCGGAC TATATCCCAA AGAAAATGAA GAAGCATGTT 
GAAGGGAAGC CGCTGAAGGG GAAGGATAGG GTCGACGGGT CTGGCTATGG TTCTACTCTT 
CCTCCTGAGA AACTGCCCTA CTTAGTAGAG TTAAGTCCAG GGAGAAGGAG TCACTTTGCC 
TACTGCAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC 
CGCCTTCAAT TAAGTGTGAC TGAAGTCGGG ACAGAGAAGT TCGATGACAA TTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTCA CTAACATGGA TGGAGTGGTC 
ACTGTGACAC CCAGAAGTGT GGATGCAGAG ACGTACGTGG ATGGCCAGCG CATCTCGGAG 
ACGACCATGC TGCAGAGCGG CATGACAGTG CAGTTCGGCT CCTCACATGT GTTCAAGTTT 
GTGGACCCCA TTCAGGACCA CGCTCTTTCG AAGAGGTCTG TGGATGGAGG CCTGATGGTC 
AAGGGCCCAA GACATAAACC TGGAGCCATT CAGGAGACAA CCTTTGATTT GGGAGGAGAT 
ATTCACAGTG GGACAGCATT GCCAGTAAGC AGGAGTACTA CTAGACTGGA CAGTGACAGA 
GTGTCCTCTG CCTCCAGCAC AGCTGAGCGA GGGATGGTGA AGCCAATGAT CCGCCTGGAC 
CAGGAGCAGG ATTACCGACG AAGAGAAAGC AGAACTCAGG ATGGGGCTGG GCCTGAACTG 
ACACTGCCTG CCAGCGTTGA ATTCAGGGAA AGCTCTGAAG ATTCTTTTTT ATCTGCCATT 
ATAAACTATA CCAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTGTTATAC 
ATGGCGTGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC GACAGAGCGC 
ACACATAAGG CCATTGCTGT CGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAAAAG AACATTGCAG GGGCGCTTGC GTTCTGGATG 
GCGAATGCAT CTGAACTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACA 
CTAGATGCCC AGGACGTCTT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAC 
TGTCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC AGAGGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACAC TCACAGGAGC CATGTCCTTG 
CTGAGGCGCT GCAGAGTCAA TGCTGCCCTG ACCATCCAGC TGTTCTCTCA GCTTTTCCAT 
TTTATCAACA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGACTCGGG CCTGTGCTCC 
CACTACTGGG GAGCGATTAT CCGCCAGCAG CTAGGGCACA TTGAAGCCTG GGCGGAGAAG 
CAGGGCCTGG AGCTGGCAGC TGACTGTCAC TTGAGCAGGA TTGTGCAGGC TACTACTTTG 
CTTACCATGG ATAAGTATGT ACCTGATGAC ATTACAAATA TAAACAGTAC GTGCTTTAAG 
TTAAATTCTC TGCAACTTCA AGCCTTGTTA CAGAACTACC ACTGTGCACC CGATGAGCCT 
TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG 
CTTGCCCGCA GTGACGGCAG AGATGTGCAG TTGGAGGAGG ACCCAGACCT ACAGCTACCC 
TTTCTCTTGC CAGAAGATGG CTATTCCTGT GATGTCGTCA GAAACATTCC AAATGGTTTA 
CAAGAGTTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GATTGGTTCC TCATACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTATGCGT 
GAGAACACAG AACTGGCCCA GCCTCTGAGG AAGGAACCTG AGATAATCAC GGTGACCCTA 
AAAAAACAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG CCAGGATAAA 
CTGGGAATCT ACGTGAAGTC TGTTGTGAAA GGAGGAGCTG CAGATGTGGA TGGACGTCTA 
GCTGCTGGGG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACCAGT TCAGTGGTCA CACTTGAAGT TGCAAAGCAA 
GGCGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGCGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT 
AATAATTCAG GTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT 
GAGCCAAAGA AACTTCCTGG TGACGACAGG CTGATGAAAA ACAGAGCAGA TCACCGTTCC 
AGCCCTAATG TGGCAAATCA GCCTCCTAGT CCTGGAGGGA AGAGTCCATA TGCCTCTGGA 
ACAGCGGCTA AGATAACATC TGTCTCCACT GGAAACCTGT GCACTGAGGA GCAGACCCCT 
CCACCTAGAC CTGAAGCCTA TCCCATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC 
TTTCCAGCTT CAAAATCCCA GGACCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT 
ACCCGTTCCC AGGAAGAGCT TCGAGAAGAG AAGGGTTACC AGCTTGAGCG GCATCGAGCA 
GAGGCTGGCA TGGATCGCAA GTGTGACAGT GACATGTGGA TCAATCAGAG CTCCTCAGTG 
GATTCCAGCA CATCCAGCCA AGAGCACCTG AACCACTCCT CCAAGTCAGT CACCCCTGCT 
TCCACTCTGA CCAAAAGCGG TCCCGGACGT TGGAAAACCC CCGCAGCAGT ACTGCCTACC 
CCTGTGGCTG TCTCCCAGCC CATTCGAACA GACCTCCCTC CCCCACCCCC TCCGCCTCCT 
GCCCACTACA CCAGTGATTT TGATGGCATT CCAGTGGATC TGCCCCTCCC ACCTCCTCCT 
GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGAGGAA AAAGAGAGAA 
GAGCATCAGC GGTGGTATGA GAAAGAGAAG GCCCGCCTCG AGGAGGAGCG CGAGAGGAAG 
CGCAGGGAAC AGGAGCGGAA GTTGGGGCAG ATGCGCACGC AGTCCCTGAA CCCTGCTTCC 
TTCTCTCCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA 
GAAACAGTCA TCCGGGAGCT GCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAGGGAC 
CTGCAGTACG TCACCATCAG TAAAGAAGAG CTGTCCTCTG GGGACAGTCT GTCTCCAGAC 
CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAGC AGCAAATGCA CATCGTCGAC 
ATGCTGAGCA AGGAGATCCA GGAACTGCAG AACAAGGTGG ACCGCACCGC AGAGGAGAGT 
GACCGCCTGC GGAAGCTTAT GCTGGAATGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAAGGATG AGGATGACGA GGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG 
CAGCGTCTGG AGGCTGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGGATG AGGAGCGCAG GCGCCAGCAG CAGTTGGAAG AGATGCGCAA ACGAGAAGCA 
GAAGATCGTG TAAGACAAGA GGAAGATCGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA 
GATGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG 
CGCAGACAGC ACGACGAGGC AGCACGAAGG TTGCTGGAGC CCGAAGAGCC TGGGCTGAGC 
CGACCTCCAC TTCCACGGGA CTATGAGCCC CCATCCCCGT CCCCAGCACC CAGCGCCCCT 
CCTCCCCCAC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATATGAT GATGATGAGG AGGAGGACTG CGGCCCAGCA 
GGTCAGGATA AGTACCCCAG CACAAGGAGG TCTCATGAGC ACCTTCCTCT TGCTGCCCCT 
AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTT TCTCTCCAAC 
TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACTGCTA ACAAAGCAGG CCAGCCCCCA 
CCCCCTCCAG GCAAACCGAG CTTCTGCCGT CCTGGCCCCT GGAAAGGTAG AGGACCACAC 
TCTTATACGG GATCTGCTGG AACAGCTTCA GCAGTCCATG ATGTTCCTCG GGACCCAAGA 
GAGAAGCACA CTAGAAGCCA AGATGCAGAC TTTCCAGGCA GTTCTGGAGC CCCTGAAAAC 
CTGACATTTA AAGAGCGTCA GCGCCTCTTT TCACAAGGTC AAGATGTGTC TGACAAAGTA 
AAAGCTTCTC GTAAATTAAC AGAACTTGAG AATGAACTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021090324.1
CDS	122..5872
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQDKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDSADFAVAESLEKYGLEKESPKDYCIARVMLPPGAQHSDERGAKEVILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRVDGSGYGSTLPPEKLPYLVELSPGRRSHFAYCSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSVDAETYVDGQRISETTMLQSGMTVQFGSSHVFKFVDPIQDHALSKRSVDGGLMVKGPRHKPGAIQETTFDLGGDIHSGTALPVSRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDGAGPELTLPASVEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDITNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSGQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYASGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTESNHSSIAIQRVTRSQEELREEKGYQLERHRAEAGMDRKCDSDMWINQSSSVDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGIPVDLPLPPPPANQAGPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYVTISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIQELQNKVDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRKREAEDRVRQEEDRRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEEPGLSRPPLPRDYEPPSPSPAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDEEEDCGPAGQDKYPSTRRSHEHLPLAAPKPQQPPCQPRPASDGIFLSNSFQPPLAKANSTANKAGQPPPPPGKPSFCRPGPWKGRGPHSYTGSAGTASAVHDVPRDPREKHTRSQDADFPGSSGAPENLTFKERQRLFSQGQDVSDKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_021234665.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus afadin, adherens junction formation factor (Afdn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021234665.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATCT GGAGTTTCAT 
GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAGTGCATT 
CGGGTATCTA GCACGGCAAC TACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC 
CCTGATATGC GCATGCTGTC CTCTCCCAAG TATTCTCTCT ATGAAGTGCA TGTCAGTGGA 
GAAGAAAGAA GATTAGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA CTGGAACAAA 
GATGATCGGG AAGGTAGATT CGTCCTTAAG AATGAGAATG ACGCCATTCC AGCCAAGAAG 
GCTCAGAGTA ATGGACCCGA GAAGCAGGAC AAGGAAGGCG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGGG AAAAAGAGGC ATTGAGACAA 
GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAATTC TCGACTGGCT 
GCTGAAGTTT ACAAAGATAT GCCTGAAACC AGCTTTACGC GAACAATTTC TAACCCTGAG 
GTGGTTATGA AACGGCGGCG GCAGCAAAAG CTTGAAAAGA GAATGCAAGA GTTTCGGAGC 
TCAGATGGGC GGCCTGACTC AGGTGGAACA TTGAGAATAT ATGCAGACAG TTTAAAACCA 
AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACAGACT CTGCAGACTT TGCTGTGGCG 
GAATCTCTGG AGAAGTATGG TCTAGAAAAA GAGAGTCCCA AGGACTACTG CATTGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAGAG GTGCTAAGGA AGTTATTCTT 
GATGATGATG AGTGTCCTTT GCAGATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCGGAC TATATCCCAA AGAAAATGAA GAAGCATGTT 
GAAGGGAAGC CGCTGAAGGG GAAGGATAGG GTCGACGGGT CTGGCTATGG TTCTACTCTT 
CCTCCTGAGA AACTGCCCTA CTTAGTAGAG TTAAGTCCAG GGAGAAGGAG TCACTTTGCC 
TACTGCAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC 
CGCCTTCAAT TAAGTGTGAC TGAAGTCGGG ACAGAGAAGT TCGATGACAA TTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTCA CTAACATGGA TGGAGTGGTC 
ACTGTGACAC CCAGAAGTGT GGATGCAGAG ACGTACGTGG ATGGCCAGCG CATCTCGGAG 
ACGACCATGC TGCAGAGCGG CATGACAGTG CAGTTCGGCT CCTCACATGT GTTCAAGTTT 
GTGGACCCCA TTCAGGACCA CGCTCTTTCG AAGAGGTCTG TGGATGGAGG CCTGATGGTC 
AAGGGCCCAA GACATAAACC TGGAGCCATT CAGGAGACAA CCTTTGATTT GGGAGGAGAT 
ATTCACAGTG GGACAGCATT GCCAGTAAGC AGGAGTACTA CTAGACTGGA CAGTGACAGA 
GTGTCCTCTG CCTCCAGCAC AGCTGAGCGA GGGATGGTGA AGCCAATGAT CCGCCTGGAC 
CAGGAGCAGG ATTACCGACG AAGAGAAAGC AGAACTCAGG ATGGGGCTGG GCCTGAACTG 
ACACTGCCTG CCAGCGTTGA ATTCAGGGAA AGCTCTGAAG ATTCTTTTTT ATCTGCCATT 
ATAAACTATA CCAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTGTTATAC 
ATGGCGTGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC GACAGAGCGC 
ACACATAAGG CCATTGCTGT CGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAAAAG AACATTGCAG GGGCGCTTGC GTTCTGGATG 
GCGAATGCAT CTGAACTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACA 
CTAGATGCCC AGGACGTCTT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAC 
TGTCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC AGAGGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACAC TCACAGGAGC CATGTCCTTG 
CTGAGGCGCT GCAGAGTCAA TGCTGCCCTG ACCATCCAGC TGTTCTCTCA GCTTTTCCAT 
TTTATCAACA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGACTCGGG CCTGTGCTCC 
CACTACTGGG GAGCGATTAT CCGCCAGCAG CTAGGGCACA TTGAAGCCTG GGCGGAGAAG 
CAGGGCCTGG AGCTGGCAGC TGACTGTCAC TTGAGCAGGA TTGTGCAGGC TACTACTTTG 
CTTACCATGG ATAAGTATGT ACCTGATGAC ATTACAAATA TAAACAGTAC GTGCTTTAAG 
TTAAATTCTC TGCAACTTCA AGCCTTGTTA CAGAACTACC ACTGTGCACC CGATGAGCCT 
TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG 
CTTGCCCGCA GTGACGGCAG AGATGTGCAG TTGGAGGAGG ACCCAGACCT ACAGCTACCC 
TTTCTCTTGC CAGAAGATGG CTATTCCTGT GATGTCGTCA GAAACATTCC AAATGGTTTA 
CAAGAGTTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GATTGGTTCC TCATACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTATGCGT 
GAGAACACAG AACTGGCCCA GCCTCTGAGG AAGGAACCTG AGATAATCAC GGTGACCCTA 
AAAAAACAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG CCAGGATAAA 
CTGGGAATCT ACGTGAAGTC TGTTGTGAAA GGAGGAGCTG CAGATGTGGA TGGACGTCTA 
GCTGCTGGGG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACCAGT TCAGTGGTCA CACTTGAAGT TGCAAAGCAA 
GGCGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGCGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT 
AATAATTCAG GTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT 
GAGCCAAAGA AACTTCCTGG TGACGACAGG CTGATGAAAA ACAGAGCAGA TCACCGTTCC 
AGCCCTAATG TGGCAAATCA GCCTCCTAGT CCTGGAGGGA AGAGTCCATA TGCCTCTGGA 
ACAGCGGCTA AGATAACATC TGTCTCCACT GGAAACCTGT GCACTGAGGA GCAGACCCCT 
CCACCTAGAC CTGAAGCCTA TCCCATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC 
TTTCCAGCTT CAAAATCCCA GGACCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CACATATGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT 
ACCCGTTCCC AGGAAGAGCT TCGAGAAGAG AAGGGTTACC AGCTTGAGCG GCATCGAGCA 
GAGGCTGGCA TGGATCGCAA GTGTGACAGT GACATGTGGA TCAATCAGAG CTCCTCAGTG 
GATTCCAGCA CATCCAGCCA AGAGCACCTG AACCACTCCT CCAAGTCAGT CACCCCTGCT 
TCCACTCTGA CCAAAAGCGG TCCCGGACGT TGGAAAACCC CCGCAGCAGT ACTGCCTACC 
CCTGTGGCTG TCTCCCAGCC CATTCGAACA GACCTCCCTC CCCCACCCCC TCCGCCTCCT 
GCCCACTACA CCAGTGATTT TGATGGCATT CCAGTGGATC TGCCCCTCCC ACCTCCTCCT 
GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGAGGAA AAAGAGAGAA 
GAGCATCAGC GGTGGTATGA GAAAGAGAAG GCCCGCCTCG AGGAGGAGCG CGAGAGGAAG 
CGCAGGGAAC AGGAGCGGAA GTTGGGGCAG ATGCGCACGC AGTCCCTGAA CCCTGCTTCC 
TTCTCTCCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA 
GAAACAGTCA TCCGGGAGCT GCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAGGGAC 
CTGCAGTACG TCACCATCAG TAAAGAAGAG CTGTCCTCTG GGGACAGTCT GTCTCCAGAC 
CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAGC AGCAAATGCA CATCGTCGAC 
ATGCTGAGCA AGGAGATCCA GGAACTGCAG AACAAGGTGG ACCGCACCGC AGAGGAGAGT 
GACCGCCTGC GGAAGCTTAT GCTGGAATGG CAGTTCCAGA AGAGACTGCA GGAGTCCAAG 
CAGAAGGATG AGGATGACGA GGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG 
CAGCGTCTGG AGGCTGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGGATG AGGAGCGCAG GCGCCAGCAG CAGTTGGAAG AGATGCGCAA ACGAGAAGCA 
GAAGATCGTG TAAGACAAGA GGAAGATCGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA 
GATGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG 
CGCAGACAGC ACGACGAGGC AGCACGAAGG TTGCTGGAGC CCGAAGAGCC TGGGCTGAGC 
CGACCTCCAC TTCCACGGGA CTATGAGCCC CCATCCCCGT CCCCAGCACC CAGCGCCCCT 
CCTCCCCCAC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATATGAT GATGATGAGG AGGAGGACTG CGGCCCAGCA 
GGTCAGGATA AGTACCCCAG CACAAGGAGG TCTCATGAGC ACCTTCCTCT TGCTGCCCCT 
AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTT TCTCTCCAAC 
TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACTGCTA ACAAAGCAGG CCAGCCCCCA 
CCCCCTCCAG GCAAACCGAG CTTCTGCCGT CCTGGCCCCT GGAAAGGTAG AGGACCACAC 
TCTTATACGG GATCTGCTGG AACAGCTTCA GCAGTCCATG ATGTTCCTCG GGACCCAAGA 
GAGAAGCACA CTAGAAGCCA AGATGCAGAC TTTCCAGGCA GTTCTGGAGC CCCTGAAAAC 
CTGACATTTA AAGAGCGTCA GCGCCTCTTT TCACAAGGTC AAGATGTGTC TGACAAAGTA 
AAAGCTTCTC GTAAATTAAC AGAACTTGAG AATGAACTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters.
Scientific reports740472(2017 01)
McCann KE,Sinkiewicz DM,Norvelle A,Huhman KL

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Afdn cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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