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competitive labelling (differential chemical modification)

A method for characterization of the microenvironment of an amino acid residue of a protein, e.g. its accessibility to solvent or its pKa; other residues of the same amino acid serve as internal controls. Rates of reaction or ratios of reaction compared with control residues are observed; very limited amounts of reagent and extents of reaction maximize the competition for the reagent between similar groups on the protein and ensure that only unmodified protein reacts with reagent.

Related Biology Tools

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Service and Products

Protein Expression

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Insect Expression

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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

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